900 results about "Fermentation system" patented technology

The invention discloses a compound microbial agent for enhancing production of beta-phenethyl alcohol in a white spirit brewing process as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of microorganisms. The compound microbial agent is prepared from aspergillus niger, pichia membranaefaciens, Kazakstan monospora, saccharomyces cerevisiae and sporospora delbrueckii; in the compound microbial agent, the final concentration OD600 of the aspergillus niger, the final concentration OD600 of the pichia membranaefaciens, the final concentration OD600 of the Kazakstein monospora, the final concentration OD600 of the saccharomyces cerevisiae and the final concentration OD600 of the sporospora delbrueckii are 0.8-1.5, 0.8-1.5, 0.5-1, 0.8-1.5 and 0.5-1 respectively. After the compound microbial agent provided by the invention is applied to a solid-state fermentation system of Luzhou-flavor liquor, the yield of the aroma substance beta-phenethyl alcohol can be obviously increased by adjusting the eukaryotic microbial community structure of the fermentation system; the method can effectively solve the problem that the stability of the method for producing beta-phenethyl alcohol in the process of intensifying Baijiu fermentation by a single strain is poor.

The invention discloses a biogas fermentation purification liquefaction and carbon capture process. The process comprises a biogas fermentation system for performing high-temperature fermentation on biomass; the biogas purification and liquefaction system, the CO2 purification and liquefaction system and the organic fertilizer production system are arranged at the biogas residue output end of the biogas fermentation system to produce organic fertilizer; a thermal energy recovery system; according to the invention, a high-temperature facultative anaerobic hydrolysis and high-temperature anaerobic fermentation process is provided, so that high-concentration biomass biogas is produced; cH4, H2S and CO2 in the biogas are separated and liquefied by adopting a process of'wet process and dry process serial desulfurization, PSA (Pressure Swing Adsorption) + MDEA (Methyl Methanol Ether Alcohol) decarburization, molecular sieve dehydration, single-order two-stage mixed refrigerant compression and propane refrigeration compression ', and produced liquid CH4 and CO2 are recycled; meanwhile, the biogas residues are subjected to a secondary fermentation technology under the action of microorganisms to obtain a solid organic fertilizer; in addition, the solar heat collection and heat pump technology is utilized, and the whole-field heat energy is recycled to supply heat to the biogas fermentation system.

The invention discloses a salt-tolerant growth-promoting bacillus cereus strain and an application thereof. In particular discloses bacillus cereus T146, and the preservation number of the bacillus cereus T146 is CGMCC (China General Microbiological Culture Collection Center) No.30429. The bacillus cereus T146 disclosed by the invention has good biofilm production, siderophore secretion and nitrogen fixation capabilities, and can remarkably increase the root length, the overground dry weight, the plant height, the number of lateral roots and the average density of the lateral roots of wheat under the condition of salt stress. According to the invention, a low-cost, efficient and applicable culture medium aiming at the bacillus cereus T146 is determined, a stable and controllable fermentation system is established, the preparation method of the microbial inoculum with high mature spore number is obtained by optimizing a spray drying process, and the active spore number of the microbial inoculum can reach 1.3 * 10 < 11 > CFU / g. The bacillus cereus T146 has good development and application prospects in the fields of microbial agents, microbial fertilizers, soil improvement and the like.

Methods of and system for growing and maintaining an optimized / ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, % DT / % Yeast by weight ratio (or “food” to yeast ratio), is used (e.g., % DT=glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

Methods of and system for growing and maintaining an optimized / ideal active yeast solution in the yeast tank and fermenter tank during the fermentation filling cycle are provided. A new yeast stage tank is used between the yeast tank and the fermenter tank allowing yeast to rapidly produce a huge amount of active young yeast cells for a fermenter during the filling period. A measurable and useful controlling factor, % DT / % Yeast by weight ratio (or “food” to yeast ratio), is used (e.g., % DT=glucose), which offers information on the health status of the yeast. The controlling factor is used to control the status of the yeast throughout the entire process.

The invention provides an inoculum domestication method for relieving acid or ammonia nitrogen inhibition of an anaerobic fermentation system, which comprises the following steps of: carrying out constant-temperature anaerobic fermentation culture at medium temperature (30-35 DEG C) or high temperature (50-55 DEG C) until the gas yield is stable by taking livestock manure, activated sludge, industrial anaerobic digestion residues or mineralized refuse biogas fermentation liquor and the like as inoculums; the obtained inoculum is an inoculum which has relatively strong acid tolerance or ammonia nitrogen tolerance after domestication. The domesticated inoculum with good tolerance and the original untreated inoculum are mixed according to a certain proportion and then put into an anaerobic fermentation system, and wet fermentation (TS = 5-10% and total solid content) or dry fermentation (TS = 20-30%) is carried out, so that the pH value of the anaerobic fermentation system is recovered to a state suitable for growth of methanogens.

The invention provides cheese and a preparation method thereof, and the preparation method comprises the following steps: carrying out first fermentation on a base material containing raw milk in the presence of a basic leavening agent, and inactivating the basic leavening agent to obtain a first fermentation system; wherein the basic leavening agent comprises lactobacillus bulgaricus and / or streptococcus thermophilus; and carrying out second fermentation on the first fermentation system in the presence of medium-temperature bacteria to obtain the cheese. The method can shorten the fermentation time and improve the cheese production efficiency.

The invention relates to a fermentation tank for microbial reaction, in particular to a forced internal circulation type microbubble reactor with a guide cylinder, which comprises a tank body (1), a cylindrical guide cylinder (3) is arranged in the tank body (1), an overflow weir (32) is arranged at the upper part of the guide cylinder, and more than one liquid outlet pipe is arranged at the lower part of the guide cylinder; the upper part of the tank body (1) is provided with a plug flow stirrer (2), the lower end of the plug flow stirrer is provided with a plug flow paddle (24), and the plug flow paddle is positioned at the upper part of the guide cylinder (3); and at least one microbubble generator (4) is arranged in the guide cylinder (3) and is communicated with the air guide pipe. By means of the built-in guide cylinder, the device is large in flow, small in power consumption, mild in flowing, small in shearing force on liquid, uniform in airflow mixing, convenient to distribute and more suitable for a biological fermentation system.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention relates to the technical field of Chinese herbal medicine fermentation, and discloses a mixed strain fermentation method for efficient conversion of non-medicinal parts of traditional Chinese medicine, which comprises the following steps: constructing an intelligent fermentation system comprising a plurality of independent fermentation units, respectively culturing different strains such as aspergillus niger, bacillus subtilis, saccharomyces cerevisiae and the like in each fermentation unit, the online sensor is integrated in the system and used for monitoring key fermentation parameters in real time, and a fermentation model and an optimization algorithm are arranged in the system and can automatically adjust fermentation conditions. An intelligent fermentation system comprising a plurality of independent fermentation units is constructed, precise classification pretreatment and mixing according to a specific ratio are carried out on rhizoma polygonati stems and leaves and gentian stems and leaves, and an intelligent control system is utilized to dynamically regulate and control the fermentation process, so that the synergistic effect of different strains such as aspergillus niger, bacillus subtilis and saccharomyces cerevisiae is fully exerted, and the fermentation efficiency is improved. The dissolution rate and the conversion rate of active ingredients in the non-medicinal part of the traditional Chinese medicine are improved.

The invention belongs to the technical field of wastewater treatment and energy recovery, and provides a regulation and control method for promoting anaerobic acid production by taking soybean molasses as a substrate through cooperation of ferroferric oxide and bamboo charcoal. Soybean molasses is used as a substrate carbon source, anaerobic activated sludge is inoculated in a medium-temperature SBR reactor, the volume ratio of reactor inflow sewage to sludge is 2: 1, ferroferric oxide and bamboo charcoal composite conductive particles which are subjected to ball milling treatment and have the concentration of 1 g / L are added to promote an anaerobic fermentation system to produce acid, and then anaerobic fermentation is carried out. The mass ratio of ferroferric oxide to bamboo charcoal in the ferroferric oxide and bamboo charcoal composite conductive particles is (1: 2)-(2: 1); monitoring the acid yield, the pH value change and the product type; and the variety and concentration of the acid-producing substance are analyzed, and the interaction of all the components in the reaction process is evaluated. The acid production rate and the total acid production amount in the anaerobic acid production process are obviously improved. And the activity of anaerobic flora is promoted. The variety distribution of acid-producing substances is obviously optimized, and the stability of the acidification process is improved.

The invention discloses a method for promoting acid production and alcohol production through fermentation of clostridium perdali C1 gas by using a biological source carbon-based redox regulation and control medium. The preparation method comprises the following steps: obtaining biochar by adopting a high-temperature pyrolysis method, grinding and sieving to obtain a biogenic carbon-based redox regulation medium, adding the biogenic carbon-based redox regulation medium into a fermentation culture medium, inoculating clostridium perdali into a fermentation device filled with the fermentation culture medium, controlling the volume ratio of H2 to CO2 in the fermentation device to be (1.5: 1)-(2.5: 1), and carrying out anaerobic fermentation for 20-30 days to obtain the biogenic carbon-based redox regulation medium. According to the invention, a biological source carbon-based redox regulation medium is introduced into a clostridium perdali gas fermentation system, and the medium not only can enrich CO2 gas, but also can improve the mass transfer efficiency of a gaseous substrate; the method has the advantages that the method is simple and convenient to operate, extra reducing power is provided for a fermentation system, electron transfer is mediated, the CO2 utilization rate of the strain and the fermentation product generation rate are remarkably increased, secondary resource utilization of solid waste biomass is achieved, C1 gas is effectively converted into high-value chemicals, and a green, economical and sustainable development road is provided for conversion of CO2.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

The invention discloses a potato starch processing waste residue fermentation device and method. The device comprises a tank body with a sterile gas filtering discharge port, an automatic inoculation box is arranged outside the tank body, and a stirring device and an automatic gas exchange device are arranged inside the tank body; the automatic inoculation box is mounted above the side of the tank body, a bacterial liquid bottle and an alcohol lamp are arranged in the automatic inoculation box, the bacterial liquid bottle is connected with an inoculation opening communicated with the interior of the tank body through a closed pipeline and feeds bacterial liquid into the fermentation system, and the alcohol lamp is arranged below the inoculation opening and heats the inoculation opening; the automatic gas exchange device comprises a pressure sensor and a plurality of air distributors, and sterile air equipment outside the tank body is communicated with each air distributor through a sterile air inlet and inputs sterile air into the fermentation system; the pressure sensor is mounted at the sterile gas filtering and discharging port, provides a real-time air pressure signal for the PLC control system and controls the gas discharging of the sterile gas filtering and discharging port, so that the problems that the existing fermentation lacks a proper device and the fermentation efficiency is low are finally solved.

The invention discloses a nano ardealite-based saline-alkali soil modifier and a preparation method thereof, and relates to the technical field of soil improvement, the method comprises the following steps: uniformly mixing humic acid, charcoal and a halophilic microbial agent, and then fermenting at 28-32 DEG C for 115-125 hours to obtain a fermentation system; the preparation method comprises the following steps: grinding ardealite into nano ardealite, dispersing the nano ardealite under the action of a surfactant and deionized water, and carrying out surface etching by using acid to form microdot vacancies on the surface of the nano ardealite, so as to obtain chemically modified nano ardealite; the nano phosphogypsum-based saline-alkali soil modifier is obtained by uniformly mixing the chemically modified nano phosphogypsum with a fermentation system, has the advantages of multiple effects, low cost and environmental friendliness, can significantly reduce the salinity and alkalinity of soil, improve the soil structure and promote the growth of crops, realizes the resource utilization of the phosphogypsum, and reduces the environmental pollution.

The present invention belongs to the technical field of microbial fermentation, and discloses the application of a compound bacterium agent of Lactobacillus rhamnosus and Bacillus licheniformis for reducing acids and higher alcohols, Lactobacillus rhamnosus, and in Baijiu. Lactobacillus rhamnosus L21 has a preservation number of: CGMCC NO. 32609, a preservation date of: November 13, 2024, and a preservation unit of: China General Microbiological Culture Collection Center. The use of the compound bacterium agent of Lactobacillus rhamnosus L21 and Bacillus licheniformis XDNZ_Bl_27 can effectively reduce organic acids such as lactic acid and propionic acid in the Baijiu fermentation system, and at the same time can also reduce the content of n-propanol in the fermented grains, which is beneficial to the regulation of acidity and higher alcohols in the process of Baijiu production.

The invention relates to a rural cultivation wastewater fermentation system, and belongs to the field of wastewater treatment equipment, the rural cultivation wastewater fermentation system comprises solid-liquid separation equipment and a fermentation tank, a compost fermentation chamber is arranged above the fermentation tank, and the compost fermentation chamber is provided with a feed port and a discharge port; a liquid outlet of the solid-liquid separation equipment is communicated with the fermentation tank, and a solid outlet of the solid-liquid separation equipment is communicated with the feeding hole; a heating mechanism is arranged in the fermentation tank, a heat exchange pipe is arranged in the compost fermentation chamber, an inlet of the heat exchange pipe is connected with a liquid outlet of the solid-liquid separation equipment through a flow control valve, and an outlet of the heat exchange pipe is connected with the fermentation tank; and the temperature sensor and the flow control valve are connected with the controller. According to the invention, after solid-liquid separation is carried out on rural cultivation wastewater, the wastewater is introduced into the fermentation tank for anaerobic fermentation, so that biogas can be generated; solid excrement, straw residues and the like enter the composting fermentation chamber and are subjected to composting fermentation, so that organic fertilizer can be obtained, and simultaneous fermentation of wastewater and solid wastes is realized.

The invention relates to the technical field of microorganisms, in particular to an ethyl carbamate degrading bacterium and application thereof.The ethyl carbamate degrading bacterium is pichia kudriavzevii K44 and is preserved in the China Center for Type Culture Collection on April 25, 2025, the preservation date is April 25, 2025, and the biological preservation number is CCTCC NO: M 2025887. The ethyl carbamate degrading bacterium can obviously degrade ethyl carbamate in a yellow rice wine fermentation system, and a scientific basis is provided for safe production and quality improvement of traditional fermented food.

The present invention belongs to the technical field related to microorganisms, and specifically relates to a preparation method and application of a lysate of bamboo leaves fermented by brewer's yeast. The present invention discloses a preparation method of a lysate of bamboo leaves fermented by brewer's yeast, comprising the following steps: preparing seed liquid; inoculating the seed liquid into a fermentation culture medium as a fermentation system for fermentation to obtain a fermentation liquid; the fermentation culture medium is composed of bamboo leaf powder, glucose and deionized water; filtering and centrifuging the fermentation liquid, homogenizing and centrifuging, sterilizing and centrifuging, and the obtained supernatant is a lysate of bamboo leaves fermented by brewer's yeast. The present invention also provides the use of the above-mentioned bamboo leaves fermented by brewer's yeast, which is any of the following: preparing antioxidant substances, preparing whitening substances, preparing antibacterial substances, and other active substances.

The invention discloses a fermentation system based on green seedling rotten vegetable, which is characterized by comprising a crushing system, a fermentation system and a solar energy supply system, the crushing system adopts a crusher integrating spiral feeding extrusion, reamer crushing and sieve pore filtration; the solar energy supply system comprises a solar heat collection device, a high-level oil tank, an energy storage facility, an energy storage medium, a heat exchanger, a water storage tank and a circulating pump which are integrated into a whole; the fermentation system comprises a plurality of vertical tank bodies, hot water jackets and heat preservation layers are arranged outside the vertical tank bodies, and the hot water jackets are circularly communicated with the solar energy supply system through heating pipelines and water outlet pipelines. The heat storage system is low in cost, but has high-capacity heat storage performance, total nutrient recovery of the waste vegetable seedlings is achieved, waste is converted into a total organic fermentation fertilizer, and soil nutrients are accurately supplemented.

The present application relates to the technical field of microbial fermentation, and particularly relates to a universal fermentation device, a yogurt production system and a method for producing a yogurt with reduced uric acid. The universal fermentation device comprises a fermentation tank, a sampling tube, a test tube, a sliding member, a pressure detection module and a processor. The pressure detection module is installed on the test tube. The sliding member is slidingly fitted on the test tube, and the sliding member is connected with a first driving rod. The sliding member is provided with a clearance opening, and the clearance opening is matched with a plugging block. The plugging block is connected with a test rod and a second driving rod. One end of the sampling tube is communicated with the fermentation tank, and the other end is communicated with the test tube. The processor is used for determining the density of the fermentation sample and the viscosity of the fermentation sample. The universal fermentation device can realize continuous monitoring of the fermentation system during the fermentation process, effectively avoids sample confusion, improves the timeliness of analysis, ensures that the conditions reflected by the sample are highly consistent with the original fermentation system, and is suitable for various fermentation scenes.

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to shea butter fermentation liquor as well as a preparation method and application thereof. Lactobacillus plantarum, lactobacillus fermentum and lactobacillus gasseri in a specific proportion are adopted to construct a composite fermentation system, acid-resistant aspergillus niger lipase is combined, and shea butter is treated through a one-step fermentation process. By means of the synergistic effect of the three strains and the synergistic effect of the enzyme and the strains, efficient fermentation can be achieved, the content of active ingredients such as polysaccharide, amino acid and polypeptide in fermentation liquor is remarkably increased, meanwhile, the technological process is simple and stable, and the infectious microbe pollution risk is avoided. According to the fermentation liquor prepared by the process, on the basis of enhancing the original moisturizing and soothing effects of the shea butter, the problems of greasy skin feeling and poor permeation and absorption of the shea butter are effectively solved, the shea butter is further endowed with new functions of repairing skin barriers and balancing skin micro-ecology, and the application values of the shea butter and the fermentation liquor are greatly improved.

The invention relates to the technical field of veterinary drug raw materials and provides a veterinary drug raw material fermentation system and method.The veterinary drug raw material fermentation system comprises a supporting frame, a fermentation tank is arranged at the top of the supporting frame, and a handle is arranged on the side face of the supporting frame; a filtering device is arranged on the side face of the supporting frame and comprises a fixing plate, the fixing plate is fixedly connected to the side face of the supporting frame, a double-head motor is fixedly connected to the side face of the fixing plate, and an output shaft of the double-head motor is fixedly connected with a rotating rod. Through the technical scheme, the problems that in the prior art, the effect that a knocking rod continuously knocks a filter plate while a fermented raw material is filtered is difficult to achieve, the resistance of liquid in the filtering process is difficult to effectively reduce, the filtering speed is reduced, the production efficiency is reduced, continuous and stable filtering is difficult to ensure, the quality of veterinary drug raw materials is reduced, and the production cost is reduced are solved. The drug effect of a final product is difficult to ensure.

The invention discloses a dry-type high-pressure anaerobic fermentation system and method for agricultural waste, and belongs to the technical field of biogas fermention.The dry-type high-pressure anaerobic fermentation system comprises a mixing pool, a feeding tank and an anaerobic fermentation reactor, and the feeding tank is connected with a hydrolysis pool feeding pipeline and an acid production pool feeding pipeline; the anaerobic fermentation reactor comprises a hydrolysis tank, an acid production tank and a methane production tank which are separated by a reactor outer wall, a first separation wall and a second separation wall which are arranged in a concentric circle, and a biogas collection area formed by a separation top plate and a reactor dome is arranged at the top end of the reactor outer wall; an overflow channel is formed at the top end of the first separating wall; a plurality of separating wall supporting columns are circumferentially and uniformly distributed at the bottom of the second separating wall; a leachate tank is correspondingly arranged at the bottom end of the methanogenesis tank, and a plurality of biogas slurry lifting pipes are arranged in the methanogenesis tank; a fermentation waste discharge pipeline is arranged on the side wall of the second separation wall, a biogas discharge pipe is arranged on the dome of the reactor, and the fermentation system is stable in operation, small in consumption of circulating inoculum and high in volume gas production rate.

The invention relates to a pre-fermentation method for improving biogas production of straws and a biogas production fermentation system. The pre-fermentation method comprises the following steps: S1, preparing a mixed microbial agent to be fermented; the mixed microbial agent is prepared by inoculating the following zymophyte in parts by weight into a fermentation culture medium according to the total inoculation amount of 3-10% (v / v): 5-50 parts of bacillus subtilis, 10-20 parts of white-rot fungi, 5-10 parts of aspergillus oryzae, 5-10 parts of trametes versicolor and 5-10 parts of trichoderma viride; s2, uniformly mixing the mixed microbial agent with the micro-nano bubble water, and fermenting to form an enhanced hydrolysis bacterium solution containing a microbial agent fermentation solution and an enzyme solution; s3, spraying enhanced hydrolysis bacteria liquid onto the straws for enhanced hydrolysis so as to complete pretreatment before fermentation and biogas production; wherein the volume of the reinforced hydrolytic bacteria liquid required by each kg of straw is 3-5L. Compared with the prior art, the mixed microbial agent and the micro-nano bubble water are used for carrying out cooperative treatment on the straw, so that the gas production efficiency and the methane production amount of the straw can be effectively improved.

The invention belongs to the technical field of bioengineering, and particularly relates to a biomass fermentation safety reinforcement learning control method and system, and the method comprises the steps: training an initial strategy network through imitation learning by employing historical data, and providing safety weight initialization for a reinforcement learning agent; and constructing a virtual training environment fusing the mechanism model and the data-driven residual error correction network, applying domain randomization, training an agent in the environment to maximize long-term accumulated rewards, and obtaining a robust control strategy. A training strategy is deployed to a real fermentation system, a control instruction is corrected in real time through an independent safety layer according to a hard constraint rule to ensure operation safety, and meanwhile online fine tuning and updating are conducted on the strategy based on actual operation data. According to the method, safe and smooth transition from virtual training to practical application is realized, and the final ethanol concentration and the overall control performance in the fermentation process are remarkably improved.

The invention relates to the technical field of fermentation systems, and particularly discloses a partition stirring dry type anaerobic intelligent fermentation system which comprises a dynamic reconstruction fermentation unit, a bionic stirring device, a multi-dimensional sensing network, a cognitive control center, an intelligent energy network and a digital twinborn interaction system. The dynamic reconstruction unit and the bionic stirring device improve the fermentation effect; the multi-dimensional sensing network and the cognitive control center realize accurate monitoring and regional optimization; the intelligent energy network improves the energy efficiency and reduces the cost and emission; the digital twin interaction system is combined with AR and block chain evidence storage, so that operation and maintenance are facilitated, and data are guaranteed; the regional function dynamic allocation and optimization algorithm enhances the adaptability and anti-interference performance of the system, and assists the fermentation system to become efficient, intelligent and green.

The invention discloses a pretreatment method for increasing the yield of anaerobic fermentation methane of a lignocellulose material, and belongs to the technical field of high-efficiency anaerobic fermentation methane production of organic solid waste. The method comprises the following steps: (1) crushing straws; (2) mixing the straw powder and deionized water according to a mass ratio of 1: (19-21), stirring at a rotating speed of 120-180r / min, treating for 22-26h, fully and uniformly mixing, adjusting the pH value of the system to 3.4-3.7, adding 0.08-0.12 wt% of cellulase according to the mass of the straw, and carrying out enzymolysis at 49-51 DEG C for 2-4d to obtain an enzymatic hydrolysate; (3) yeast pre-fermentation: inoculating 4-6% v / v activated angel high-activity dry yeast into the enzymatic hydrolysate in the step (2) for anaerobic pre-fermentation, wherein the pre-fermentation time is 16-20 hours; and (4) anaerobic fermentation for methane production: adding 20-30% v / v of introduction sludge into the fermentation system in the step (3), and carrying out anaerobic fermentation for methane production, wherein the fermentation temperature is 36-38 DEG C. Compared with the prior art, the method has the advantages of being high in adaptability, short in treatment period, good in economic benefit, free of environmental pollution and the like, and has certain guidance and inspiration effects on engineering of producing methane from straw.

The invention relates to a method for producing 6 '-fucosylated chitosan disaccharide GlcNacbeta1-4 (Fucalpha1-6) GlcNAc through double-bacterium coupling fermentation, and belongs to the technical field of fermentation engineering. The method comprises the following steps: taking fucose and N-acetyl-D-glucosamine as substrates in a fermentation system; the 6 '-fucosylated chitosan disaccharide is produced through coupled fermentation of an engineering escherichia coli strain and yeast, and a GlcNAcbeta1-4 (Fucalpha1-6) GlcNAc synthesis pathway including N-acetylhexosamine 1-kinase nahK, fucosyltransferase nodZ, L-fucose kinase Fkp and N, N-diacetylchitosan phosphorylase Chbp is constructed in the engineering escherichia coli, so that the 6'-fucosylated chitosan disaccharide is obtained. According to the invention, the yeast is added to realize cyclic regeneration from GMP to GTP, so that not only is the problem of high oligosaccharide production cost caused by pure exogenous supplement of GTP effectively solved, but also efficient production of GlcNAcbeta1-4 (Fucalpha1-6) GlcNAc is realized, and the yield of GlcNAcbeta1-4 (Fucalpha1-6) GlcNAc is increased to 25.50 g / L by an optimized coupling fermentation production technology. The invention provides a novel synthesis technology of complex N-glycans, and has a wide application prospect.

The invention provides a carbon-manganese-based composite material as well as a preparation method and application thereof. The carbon-manganese-based composite material is a MnO2-BC composite material formed by MnO2 and biochar, and MnO2 nanoparticles are loaded on the surface of the biochar; by adding 60mg of MnO2-BC / g.VS into a WAS anaerobic fermentation system, the anaerobic fermentation performance of the WAS can be greatly improved, and the degradation rates of polysaccharide, protein and SCOD (soluble chemical oxygen demand) are improved; the method can promote the directional enrichment of Desulfovibrio and Geobacter from the angles of energy metabolism regulation and electron directional transduction optimization, stably and efficiently solves the bottleneck problems of easy acid accumulation, poor flora synergistic effect, easy environmental stress in methane production and the like in the traditional WAS anaerobic fermentation, and has excellent application potential.