Walnut lactic acid bacteria fermented beverage and preparation method thereof
A technology of lactic acid bacteria fermentation and walnut milk, applied in dairy products, food preparation, milk preparations, etc., can solve the problems of not giving full play to the advantages of walnut resources, nutrition and health care, and low added value of products, and achieve high antioxidant activity and Nutritious and health-care function, effect of increasing added value
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Embodiment 1
[0023] Weigh 100g of defatted walnut meal after screening and removing impurities, put it into hot water at 90°C for 3 minutes, filter it, put it into 1000mL of hot water heated to 80°C for 10 minutes, and beat it with a mixer for 5 minutes. Filter twice with 100-mesh filter cloth, and obtain pure walnut milk after separating pulp and residue. The prepared walnut milk was homogenized and refined under the conditions of 65°C and 20MPa, sterilized at 65°C for 25min, and cooled for later use.
[0024] Select 10% skim milk medium to activate the lactic acid bacteria strains to ensure that the titrated acidity reaches above 70°T, then the strains are activated to the required vigor. Prepare 10 mL of acclimatization medium with walnut milk and skimmed milk in a volume ratio of 0:10, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, and 10:0 , and are labeled as ①②③④⑤⑥⑦⑧⑨. Add activated lactic acid bacteria to No. ① medium, the amount added is 3-5% of the volume of No. ① medium, inoculate into No...
Embodiment 2
[0029] Weigh 10Kg of degreased walnut meal after screening and removing impurities, put it into a stainless steel tank after cleaning, add hot water heated to 90°C for 4 minutes, stir continuously, and finally filter with a 100-mesh filter cloth. Put the blanched and filtered walnut meal into 100L of hot water heated to 80°C for 10 minutes, and then use a stainless steel bedroom refiner to beat for 10 minutes. To obtain pure walnut milk, homogenize and refine the pure walnut milk in a homogenizer set at 65° C. and 20 MPa parameters, heat to 65° C., sterilize for 25 minutes, and cool for later use.
[0030] Acclimate the lactic acid bacteria strain by the method among the embodiment 1. Take 3L of the above-mentioned sterilized pure walnut milk, add 150g of sucrose and stir evenly, add 90ml of domesticated lactic acid bacteria culture solution, and incubate at a constant temperature of 43°C for 8 hours to make a production starter that is sufficient for use.
[0031] Take 90L o...
Embodiment 3
[0034] Get the walnut lactic acid bacteria fermented beverage (C=0.988g / mL) obtained in Example 1, and prepare sample solutions diluted to 0.004, 0.008, 0.02, and 0.04 times, respectively. Do blank control with ethanol, measure the DPPH free radical scavenging rate of the sample solution of different dilution multiples, prove that the present invention has the ability of removing DPPH free radicals, and the dilution multiple of DPPH free radical scavenging rate and walnut lactic acid bacteria fermented beverage is linear relationship (attachment figure 2 ).
[0035] Take the walnut lactic acid bacteria fermented beverage (C=0.988g / mL) obtained in Example 1, and prepare sample solutions diluted to 0.2, 0.4, 0.6, and 1 times respectively. With pure water as blank control, measure the sample solutions of different dilutions in the Fenton system in the hydroxyl radical scavenging rate, prove that the present invention has the ability to scavenge hydroxyl free radicals, and the hy...
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