The invention discloses a separation and purification method for improving
protein purity and emulsifying property of
fusarium venetii based on a
cell wall simplification
engineering strategy, and belongs to the technical field of
food processing and
protein separation and purification. The method for separating and purifying the
Fusarium venanii
protein comprises the following steps: dispersing
Fusarium venanii fermented hyphae in water, and adjusting the pH value to 10-12 to obtain a
Fusarium venanii solution; performing high-pressure homogenization, collecting supernate, adjusting the pH value to 4.4-4.5, performing
centrifugation, and taking protein precipitate; redissolving the protein precipitate in water, adding
chitinase for reaction, carrying out acid
precipitation again after the reaction is finished, centrifuging, and taking the protein precipitate; and redissolving the protein precipitate in water, adjusting the pH value, dialyzing and
drying to obtain the
fusarium venanii protein. The
fusarium protein obtained by the method is high in purity, has the characteristics of higher
solubility, high interfacial activity, emulsifying property and the like, and is beneficial to food industrial application of the fusarium protein.