175 results about "Quarantine" patented technology

The invention relates to the technical field of storage intelligence, and discloses an early warning and allocation system and method for intelligent inventory of inspection and quarantine reagent consumables, and the system comprises a consumption trend extraction module, a demand prediction module, a risk early warning module, an early warning trigger module, a supply feasibility evaluation module and an intelligent allocation module. Performing seasonal decomposition on the historical consumption record to obtain a consumption trend component; periodically superposing the consumption trend component to obtain a demand prediction result; based on the demand prediction result, performing inventory risk research and judgment on the current inventory to obtain an inventory early warning index; when the inventory early warning index exceeds a safety threshold value, inventory early warning information is generated; according to the consumable demand information, carrying out feasibility evaluation on inventory data and logistics constraint conditions of the supply point to obtain a feasible allocation point; generating a deployment scheme according to the safety inventory standard of the feasible deployment point; according to the invention, the perspectiveness and accuracy of early warning and allocation of the intelligent inventory of the inspection and quarantine reagent consumables can be improved.

The invention relates to the field of immunology, and discloses a polypeptide and a kit for distinguishing bovine and sheep brucella natural infection from a vaccine immune antibody. Polypeptides as shown in SEQ ID NO: 1 and SEQ ID NO: 2 are artificially synthesized and used as antigens to coat an elisa plate and a nitrocellulose membrane, and according to response characteristics of antibody level, identification and detection of natural infection and vaccine immunity of cattle and sheep populations are realized. The peptide fragment and the detection kit provided by the invention can make up the blank of identifying bovine and sheep brucella natural infection and immune antibodies. The method solves the problem that the antibody induced by the attenuated live vaccine and the naturally infected antibody are difficult to distinguish, provides a scientific basis for quarantine and purification of Brucella, and provides a technical guarantee for preventing human diseases and animals, advancing the mouth and protecting the body health of people.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

The application discloses a reagent, a method and application of the reagent and the method for simultaneously detecting three common viruses in a fancy carp, and belongs to the technical field of fish quarantine. The application provides a reaction system and a detection method for simultaneously detecting three common viruses in a fancy carp, establishes a multiplex fluorescence PCR method, adopts a full-closed reaction, solves the problem that common PCR is prone to generating aerosols and causing false positive results of tests, has high detection sensitivity, can detect target genes with a copy number of 10 orders of magnitude at the minimum, is suitable for detecting a small amount of viruses carried in normal fancy carp or viruses in water quality environment, and can fully meet the requirements of customs quarantine prevention and control, has strong specificity, has no cross reaction to templates such as IHNV, RSIV and EHNV, and further provides a virus-like particle which can be used as a positive reference product in the multiplex system, is stable in state and beneficial to storage, can monitor an extraction process, and ensures that a test is established.

The application discloses a waste stratified sealing and recycling device for health quarantine, which comprises a rail frame vertically fixed on a base plate, a discharging device arranged on the side of the rail frame, the discharging device being capable of reciprocating lifting along the extension direction of the rail frame, a treatment bin fixed on the upper part of the other side of the rail frame, a positioning and sealing assembly arranged at the lower end of the treatment bin, the positioning and sealing assembly being capable of sealing, packing and collecting the treated quarantine waste, and the discharging device being capable of fixing a garbage can and pouring the quarantine waste in the garbage can into the treatment bin.

The utility model discloses an inspection and quarantine box which comprises a box body used for storing quarantine equipment and provided with a closed structure to provide protection; the adjustable partition plate is arranged in the box body in a sliding mode so as to be matched with the storage equipment; the number of the detachable air bags is multiple, and the detachable air bags are detachably installed on the adjustable partition plate and the inner wall of the box body; wherein each detachable air bag is provided with a triggering type micro air pump, and after the triggering type micro air pumps are triggered, the detachable air bags are expanded to reach the pressure value so as to store equipment.

This utility model discloses a port risk management entry-exit quarantine device, including a storage box with several evenly distributed rollers at the bottom. A mounting plate is located on one side of the storage box, connected to the storage box via a connecting frame. Symmetrically arranged movable plates are located at the top of the storage box, and two sets of movable plates are connected to the mounting plate via a movable component. Beneficial effects: By setting the movable component, the two sets of movable plates can be adjusted to move synchronously relative to each other, allowing animal limbs to be quickly placed between clamping plates one and two. The movable component two can move clamping plates two closer to clamping plates one until the animal limbs are clamped tightly. The movable component two uses a screw rod two to engage with a movable connecting column for secure clamping. Both movable components one and two are driven by motors, eliminating the need for manual operation and saving time and effort.

The utility model belongs to the technical field of quarantine and epidemic prevention equipment, and particularly relates to a livestock veterinary quarantine and epidemic prevention device which comprises a quarantine platform, the right end of the upper surface of the quarantine platform is fixedly connected with the bottom face of a second breast board, and the upper end of the left side face of the second breast board is fixedly connected with the right side face of a fixing frame. According to the utility model, the translation plate and the spray pipe are arranged above the quarantine platform, so that the reciprocating lead screw, the reciprocating lead screw nut and the translation plate can be used for driving the spray pipe to reciprocate left and right after quarantine is completed if the animal excretes incontinence on the quarantine platform in the quarantine and epidemic prevention process of the animal; according to the cleaning device for the animal quarantine, dirt on the grating plate is flushed through cleaning liquid sprayed out of the spraying pipe, the dirt on the grating plate is rapidly cleaned, then epidemic disease transmission caused by germs contained in excrement during follow-up quarantine of other animals is avoided, and the workload of veterinarians is also reduced.

The utility model relates to the technical field of livestock quarantine, in particular to a portable vaccine refrigerating box for livestock quarantine, which comprises a box body, a cover plate, a clamping ring, a first clamping block, a sleeve and the like. A cover plate is hinged to the top of the box body, a clamping ring is hinged to one side of the cover plate, a first clamping block is installed on the box body, a sleeve is installed on the box body, a positioning groove is formed in the sleeve, and a telescopic handle is installed on the sleeve in a sliding mode. The telescopic handle is pulled forwards, the second clamping block moves inwards after being extruded by the inner wall of the sleeve, the first elastic piece shrinks, the telescopic handle drives the second clamping block to move forwards, and when the second clamping block moves to the front end of the inner side of the sleeve, the first elastic piece resets, the first elastic piece drives the second clamping block to move inwards, and the second clamping block moves forwards. And the second clamping blocks are embedded into the positioning grooves, so that a veterinarian can easily pull the box body to move, and the fatigue degree of the veterinarian when the veterinarian carries the vaccine refrigerating box on the shoulder is reduced.

This invention discloses a livestock and veterinary quarantine and testing device, relating to the field of quarantine and testing technology. The device includes a placement base, a fixing frame mounted on top of the base, and a power rod driven by an electric push rod mounted on the bottom of the fixing frame. A blood sampling tube body is mounted on the bottom of the power rod. When an animal is placed on a placement plate within the base, the electric telescopic rod is activated. This, along with a moving rod, an arc-shaped outer plate, an elastic telescopic rod, an arc-shaped inner plate, a hydraulic chamber, a force-bearing rod, a push plate, a spring, a spring, and a pressure plate, allows for initial fixation of the animal before blood sampling. Further fixation is performed during blood sampling, and the device quickly returns to the initial fixation state after sampling, reducing clamping time and minimizing the possibility of injury to the animal.

The invention provides a pine wood nematode intelligent detection method based on deep learning. The pine wood nematode intelligent detection method comprises the following steps: acquiring an original microscopic image set by using a microscopic imaging hardware system; performing basic preprocessing, key part identification and labeling and data enhancement operation on the original microscopic image set to obtain a high-quality training sample set; an improved YOLOv11 detection model is constructed; using a YOLOv11 detection model to output candidate boxes and a prediction probability, and screening and positioning the candidate boxes to obtain a double-ROI image and coordinate information; respectively extracting a tail parameter set and a closet parameter set to obtain a unified composite feature vector; and then carrying out online deployment on the YOLOv11 detection model and intuitively displaying a detection result. According to the method, key organs such as vulva caps, joint thorns and tail shapes of the pine wood nematodes can be automatically positioned and recognized under the microscopic imaging condition, and rapid judgment and auxiliary quarantine of pine wood nematode pathogens are achieved.

1. Name of the product in this design: Slaughterhouse Quarantine Hand Grip Hook. 2. Purpose of this design: for use in slaughterhouses for hooking and hanging livestock. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present invention relates to a disaster area disinfection drone, wherein the disaster area disinfection drone is controlled by a server, and the drone device is mounted on a drone mount to charge disinfectant solution and power, and unfolds the flight unit to prepare for flight, and the drone device detects the drone's location and surroundings through a sensor, flies to the disaster area, reaches the disaster area and sprays disinfectant solution, and returns to the drone mount when the disinfectant solution or power is nearly consumed or when disinfection is completed, and the drone device comprises a power unit for charging power, a flight unit for flying, a sensor unit for detecting surroundings at the top, and a disinfection unit at the bottom for storing and spraying disinfectant solution, and the drone mount is controlled by a server and may include a charging unit for charging disinfectant solution and power when the drone device is mounted on the drone mount.

The application discloses a livestock breeding poultry quarantine sampling device, and particularly relates to the technical field of poultry quarantine sampling, and comprises a positioning disc, a positioning sampling assembly is arranged on the positioning disc, the positioning sampling assembly comprises a swivel ring arranged on one side of the positioning disc, a screw rod capable of being displaced up and down is slidly connected to the middle part of the swivel ring, and a top disc is movably connected to the top end of the screw rod. The application effectively removes interfering substances in the sample, avoids the influence of impurities on subsequent pathogen detection results, improves the reliability of quarantine data, and improves the accuracy and comprehensiveness of quarantine results. The blood sample is used for detecting pathogen antibodies, immune state and other indexes, and the fecal sample is used for checking intestinal pathogens, parasite eggs and the like. The two types of samples are complementary and verified, the accuracy and comprehensiveness of the quarantine results are greatly improved, and the problem that a single sample cannot comprehensively judge the health status of poultry is effectively solved.

The invention discloses a primer probe composition, a kit and a method for detecting various cattle and sheep fever and respiratory tract pathogens based on nucleic acid mass spectrometry, and relates to the technical field of microbiological detection, a multiple PCR primer composition and a single-base extension probe group are innovatively designed, conservative stable regions in various pathogen genomes can be targeted, and the detection sensitivity is high. Single-tube multi-target synchronous amplification is realized, and further extension amplification is carried out, so that an extension product suitable for MALDI-TOF MS detection is obtained. The method realizes multi-target, high-sensitivity and automatic synchronous detection of fever pathogens of cattle and sheep, provides a basis for rapid detection of nucleic acids of multiple pathogens, and has a good application prospect in quarantine and prevention and control.

The invention belongs to the technical field of parasite sampling, particularly relates to an imported marine fish pathogenic parasite sampling and detecting device, and provides the following scheme aiming at the problems of low manual sampling efficiency, inaccurate positioning and easy sample damage in existing marine fish parasite quarantine. Comprising a sampling base, a movable supporting plate, a sampling area identification assembly, a sampling pipe and a negative pressure separation assembly. The sampling area identification assembly can losslessly and accurately position a suspected parasite infection area in a fish body. The sampling pipe is provided with an annular gas guide cavity and an annular liquid guide cavity, sterile gas and normal saline can be sprayed cooperatively, and minimally invasive and efficient stripping and recycling of insect bodies are achieved. The negative pressure separation assembly adopts a double-chamber design, a micro-filtration membrane is arranged in the negative pressure separation assembly, a sample can be shunted and independently treated in single operation, and a negative pressure liquid extraction pipe of the negative pressure separation assembly has an automatic valve function of negative pressure ventilation and normal pressure closing, so that subsequent in-situ fixation is facilitated. According to the invention, the whole process automation from precise minimally invasive sampling to automatic separation and fixation is realized.

The utility model relates to the technical field of quarantine sampling, in particular to a quarantine sample extraction device for animal quarantine, which comprises a main pipe body, a solid sampling mechanism and a liquid sampling mechanism, a guide groove is arranged in the main pipe body, a movable pipe is sleeved outside the main pipe body, and the solid sampling mechanism comprises a sampling hopper, a shear knife and an auxiliary component. The shear knife is slidably mounted at the lower end of the sampling hopper through a moving block; the auxiliary assembly comprises a first spring, an adjusting block and a second spring; the liquid sampling mechanism comprises a pull rod, a sampling cavity and a piston head, the pull rod is arranged in the guide groove, the sampling cavity is arranged at the lower end of the main pipe body, and the piston head is arranged in the sampling cavity and fixedly connected with the pull rod; by arranging the solid-state sampling mechanism and the liquid-state sampling mechanism, solid-state animal waste and liquid-state animal waste can be sampled; when the solid animal waste is sampled, crude fibers in the solid animal waste are cut off through the arranged auxiliary assembly, and the sampling efficiency is improved.

The invention discloses a rift valley fever virus nucleic acid RT-RAA-CRISPR / Cas12a detection primer probe combination, a kit and a method, and belongs to the technical field of molecular detection. In order to solve the problems that an existing RVFV detection technology is high in equipment dependence, low in sensitivity, not suitable for primary laboratories and epidemic disease sites and the like in clinical application, a highly conserved sequence of an S gene of an RVFV ZH-548 strain is selected as a detection target through comparative analysis of an RVFV S gene sequence, and the RVFV ZH-548 strain S gene is detected on the basis of the target. A group of primers and probes for RVFV nucleic acid RT-RAA-CRISPR / Cas12a visual detection are researched and developed, a corresponding detection method is established, and a matched detection kit is provided. The detection method provided by the invention is high in sensitivity, strong in specificity, simple and convenient to operate and high in safety, and has remarkable advantages in scenes such as cross-border animal quarantine, epidemic area field emergency detection and grassroots pathogen rapid screening.

The utility model relates to the technical field of quarantine boxes, and discloses a sampling box for animal quarantine, which comprises a box body and a cover body, the inner side wall of the cover body is fixedly connected with a connecting frame, one end, close to a U-shaped block, of a supporting plate is hinged with the inner wall of the cover body, and the supporting plate abuts against the adjacent side of the connecting frame. A storage cavity is formed among the supporting plate, the connecting frame and the cover body, supporting assemblies are connected to the two ends of the bottom face of the storage cavity, overturning assemblies are connected to the interiors of the two supporting assemblies, a pressure assembly is connected between the two overturning assemblies, and the middle of the pressure assembly abuts against the inner wall of the cover body. According to the adoption box for animal quarantine, paper files can be placed in the containing cavity of the cover body, the containing cavity and the box body are separated through the supporting plate, an independent storage space is formed, and then medicine and the paper files can be stored separately; and paper files in the storage cavity can be clamped and limited, so that the stability is improved, the files are prevented from shifting in the storage cavity, and the searching speed is reduced.

The utility model relates to the technical field of animal fixing devices, and discloses a fixing device for animal quarantine blood drawing, which comprises a fixing table, a supporting plate for bearing an animal body is arranged on the fixing table, a plurality of first fixing parts for fixing the animal body are arranged on the supporting plate, and the plurality of first fixing parts are lined up along the moving direction of the animal body; a hydraulic cylinder is fixedly mounted at the bottom of the fixed table, and a telescopic shaft of the hydraulic cylinder vertically upwards penetrates through the fixed table and is in sliding fit with the fixed table; telescopic shafts of the hydraulic cylinders are fixedly connected with the supporting plates; four second fixing pieces for fixing the limbs of the animal are installed on the fixing table, the supporting plate is located among the four second fixing pieces, and the supporting plate is in transmission fit with the four second fixing pieces through a transmission mechanism so as to drive the four second fixing pieces to fix the limbs of the animal at the same time. When the supporting plate rises, the four second fixing pieces can be driven to clamp and fix the four limbs of an animal at the same time, and therefore it is guaranteed that the four limbs of the animal cannot scratch medical staff.

The invention discloses a method for rapidly detecting nucleic acid of three imported bee disease pathogens through microchip integration, and relates to the technical field of pathogen detection. The method comprises the following steps: S1, acquiring DNA of a to-be-detected bee sample; s2, respectively preparing nucleic acid amplification reaction systems of EFB, AFB and CDB pathogens, wherein each system contains an exclusive primer probe and the same basic component; s3, loading the three detection systems, the negative control and the positive control to different reaction units of the same microchip; s4, executing fluorescent quantitative PCR amplification; and S5, judging the existence of pathogens according to the detection signal. According to the invention, the limitation of cross-type pathogen detection of bacteria and fungi is broken through, single synchronous detection is realized, the time is shortened, the cost of reagent consumables is reduced by more than 80% by a 1.2 mu L small system, and the kit is strong in specificity, high in sensitivity and good in repeatability, is adaptive to a conventional PCR instrument, and assists entry quarantine and bee disease prevention and control.

The invention relates to the technical field of molecular biological detection, in particular to a method for detecting DNA (Deoxyribose Nucleic Acid) of Changlin bark beetles in a sample, which can realize visual rapid detection of Changlin bark beetles based on an RPA-CRISPR / Cas12a system. The invention provides a primer combination and a specific crRNA sequence for detecting DNA (Deoxyribose Nucleic Acid) of Changlin bark beetles. The detection method disclosed by the invention is simple and convenient to operate, short in reaction time, high in sensitivity and specificity, and suitable for port quarantine, forestry monitoring and field screening, the detection result can be directly interpreted, the detection can be carried out without a heating instrument, and the limitation that traditional detection depends on laboratories and professional operation is broken through; an efficient and convenient detection means is provided, and powerful technical support is provided for early warning and precise prevention and control of the beetles.

Provided is a method for producing an antibody and the antibody produced by the method. The method includes immunizing an animal transplanted with stems cell with differentiation potential using an antigen and obtaining an antigen-specific antibody. The stem cells with differentiation potential are derived from a donor animal carrying one or more human immunoglobulin variable region gene segments. The method solves problems in the prior art including long transportation time and high costs associated with live transgenic animals used for producing human antibodies due to policy and quarantine requirements. When producing antibody against a target antigen having high homology with a transgenic animal, an immunosuppressed animal with a target antigen-encoding gene knockout is used as a transplantation recipient, which solves the problem in the prior art where transgenic animals have difficulty generating antibodies against homologous regions of the target antigen between humans and transgenic animals.

This invention discloses a multimodal image fusion method and system for monitoring pig health, belonging to the field of pig farming and animal quarantine technology. It achieves real-time detection through synchronous acquisition and spatiotemporal alignment of multimodal images, preprocessing and optimization, attention mechanism feature fusion, and spatiotemporal Transformer health status recognition, combined with an edge-center collaborative architecture. A self-optimizing closed loop is constructed through manual review and incremental training. The system is equipped with a multi-module collaborative architecture, with each module implementing image acquisition, preprocessing, feature fusion, status recognition, real-time detection, and model optimization functions. This invention integrates multimodal image information and jointly models spatiotemporal features, significantly improving detection accuracy and efficiency. It features early warning, anti-interference, and self-learning evolution, making it suitable for automated pig entry quarantine lines in large-scale farms and slaughterhouses, providing technical support for intelligent livestock disease prevention and control.

The invention relates to the technical field of molecular biology, and discloses a primer probe set for species identification of echinococcus granulosus, and the primer probe set comprises a first primer probe set for targeting echinococcus granulosus, a second primer probe set for targeting echinococcus multilocularis and / or a third primer probe set for targeting echinococcus stone. According to the primer probe group, synchronous, rapid and high-specificity identification and detection of three kinds of echinococcus, namely echinococcus granulosus (Eg), echinococcus multilocularis (Em) and echinococcus stone (Es), are realized. When the primer probe group is used for identifying and detecting the three kinds of echinococcus, the detection sensitivity reaches 200 copies / mL, and the positive coincidence rate reaches 100%. The primer probe group realizes synchronous and accurate identification of echinococcus species, and has a wide application prospect in molecular epidemiological investigation and port quarantine.

The application discloses a special temporary storage box for mollusk scanning, and relates to the field of biological quarantine imaging auxiliary equipment.The box body is provided with a plurality of supporting feet on the lower end face; a sealing door is installed on one side of the box body; a control bin is fixed to the side wall of the box body, and the inside of the control bin at least comprises a life maintaining system for delivering constant-temperature and constant-humidity gas or circulating water to the inside of the box body and storing anesthetic medium; a restraint assembly is arranged in the inside of the box body; and an inner lining assembly is fixed in the inside of the box body and located below the restraint assembly.

The invention discloses a mite killing activity evaluation method suitable for a bee colony, and relates to the technical field of bee breeding and inspection and quarantine. The method comprises the following specific steps: cleaning the bottom of a bee colony box, performing first-time mite killing treatment by using a to-be-detected acaricide, and counting the mite falling number of different bee mites; performing second-time acaricidal treatment by adopting acaricides with different action mechanisms, and counting the number of dropped bee mites of different bee mites; the sum of the mite falling number of the same bee mite after two times of mite killing treatment is the total number of the bee mites in the bee colony, the death rate of the bee mites is obtained according to the ratio of the mite falling number of the bee mites treated by the to-be-detected acaricide to the total number of the same bee mites in the bee colony, and the death rate is used for representing the acaricidal activity of the to-be-detected acaricide to different bee mites. According to the method, the quantity of the dropped mites at the bottom of the box is counted twice, so that the mite killing activity of the mite killing medicine on the bee mites can be dynamically evaluated, the actual lethal effect of the mite killing medicine on the bee mites can be directly reflected, and errors caused by uneven distribution of the bee mites and sampling deviation can be effectively avoided.