362 results about "Quarantine" patented technology

The invention discloses a rapid sequencing and traceability analysis system for input infectious diseases, which relates to the field of customs quarantine and comprises a sample preprocessing unit, a high-throughput sequencing unit, a data processing and quality control unit, a rapid comparison and annotation unit and a traceability analysis unit. According to the input infectious disease rapid sequencing and traceability analysis system, a complete link is formed from sample preprocessing, library construction, data cleaning, pathogen recognition and transmission map construction, module splitting and manual intervention are avoided, and tasks can be automatically completed in large-scale and emergency scenes.

The invention discloses a micro-fluidic chip for detecting quarantine pathogens of unprocessed grains. According to the micro-fluidic chip, each micro-fluidic chip at least comprises 8 detection channels, each detection channel at least comprises 48 reaction areas, and the 48 reaction areas are used for detecting 11 kinds of quarantine disease raw materials of unprocessed grains; the 11 raw grain quarantine disease raw materials comprise soybean northern stem canker germs, soybean stem brown rot germs, North American soybean sudden death syndrome germs, phaseolus vulgaris bacterial wilt germs, wheat tilletia indica, ryegrass Tilletia tillea, wheat streak mosaic viruses, corn bacterial wilt germs and corn fusarium wilt germs. , maize chlorotic mottle virus and tomato canker pathogen. The micro-fluidic chip provided by the invention can be used for simultaneously detecting 11 kinds of raw grain quarantine pathogens of 8 to-be-detected samples, so that the detection efficiency is improved, and the micro-fluidic chip has important significance on inspection and quarantine work of the raw grain quarantine pathogens.

The invention provides a rapid monitoring device and method for ballast water, relates to the technical field of ballast water monitoring, and provides a more reliable, rapid, effective and standardized monitoring method, a portable device and an enrichment device for entry and exit inspection and quarantine departments. The device comprises a box body, a connecting flange arranged outside the box body and an enrichment device located outside the box body and used for enriching ballast water environment DNA related substances, an inner cavity of the box body is hollow, a positive pressure pump is fixed in the inner cavity of the box body, and a negative pressure pump is fixed in the inner cavity of the box body. A water delivery port and a water discharge port are formed in the box body, the connecting flange is communicated with the water delivery port of the box body through a connecting pipe, a water inlet of the positive pressure pump is communicated with the water delivery port of the box body through a pipeline, a water outlet of the positive pressure pump is communicated with a water inlet of the enrichment device through a pipeline, and a water outlet of the enrichment device is communicated with the water discharge port of the box body through a pipeline.

The invention discloses an animal quarantine method, and aims to solve the problems of high omission ratio, early warning lagging and lack of environment optimization of the traditional quarantine means. According to the method, multi-dimensional biological signals such as the heart rate, the respiratory rate, the body temperature and the acceleration of an animal are collected in real time through an ear tag type wearable device, and the anomaly detection precision is improved through a self-adaptive sampling mechanism; meanwhile, the environment monitoring module collects the temperature, humidity and ammonia gas concentration of the farm, and dynamically regulates and controls the temperature, humidity and ammonia gas concentration in case of abnormality. And the edge computing node adopts a long-short-term memory network to fuse and analyze data, outputs an epidemic disease risk probability, and optimizes the model through self-learning. The embodiment shows that the method can perform early warning and environment optimization in the incubation period, the quarantine time is shortened to 5-10 minutes per head, the omission ratio is lower than 5%, and an efficient and accurate epidemic disease prevention and control solution is provided for the breeding industry.

The invention provides an isothermal amplification primer probe set for detecting Colletotrichum fucicola of Carya illinoensis, a rapid detection kit, a method for detecting Colletotrichum fucicola and application of the isothermal amplification primer probe set and the rapid detection kit, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current apocarya colletotrichum gloeosporioides is difficult to identify, excessively depends on expensive instruments and technicians and the like, the apocarya colletotrichum gloeosporioides LFD-RPA detection technology research is developed, the apocarya colletotrichum gloeosporioides can be simply, conveniently and efficiently detected, the apocarya colletotrichum gloeosporioides LFD-RPA has no cross reaction with 22 representative strains of the colletotrichum gloeosporioides, the specificity is high, the detection time is the lowest detection limit is 10 pg of genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

The invention discloses a health quarantine space module system and a construction method. The health quarantine space module system comprises a front-end conventional inspection area and a rear-end troubleshooting disposal area, the front-end conventional inspection area comprises a health declaration verification module, a medical inspection module and a body temperature monitoring module; and the rear-end troubleshooting treatment area comprises a medical troubleshooting module and a subsequent treatment module. The health quarantine space construction method matched with the method comprises the following processes of health declaration verification, medical inspection, body temperature monitoring, medical investigation and subsequent treatment. According to the module system, efficient connection between a traditional port building and a new-period health quarantine module is established, and the inspection process of customs clearance personnel is more convenient and efficient through more scientific streamline system setting, functional structure and air pressure control; the field operation is more scientific and orderly; the risk of cross infection caused by mixed flow of personnel is avoided, and indoor space environment pollution caused by gas flow is avoided.

The invention relates to the technical field of storage intelligence, and discloses an early warning and allocation system and method for intelligent inventory of inspection and quarantine reagent consumables, and the system comprises a consumption trend extraction module, a demand prediction module, a risk early warning module, an early warning trigger module, a supply feasibility evaluation module and an intelligent allocation module. Performing seasonal decomposition on the historical consumption record to obtain a consumption trend component; periodically superposing the consumption trend component to obtain a demand prediction result; based on the demand prediction result, performing inventory risk research and judgment on the current inventory to obtain an inventory early warning index; when the inventory early warning index exceeds a safety threshold value, inventory early warning information is generated; according to the consumable demand information, carrying out feasibility evaluation on inventory data and logistics constraint conditions of the supply point to obtain a feasible allocation point; generating a deployment scheme according to the safety inventory standard of the feasible deployment point; according to the invention, the perspectiveness and accuracy of early warning and allocation of the intelligent inventory of the inspection and quarantine reagent consumables can be improved.

The invention relates to the technical field of animal quarantine, and discloses an animal quarantine data management method and system, and the method comprises the steps: obtaining original animal quarantine data, carrying out the preprocessing of the original animal quarantine data, obtaining the processed animal quarantine data, and carrying out the processing of the processed animal quarantine data based on a K-means clustering algorithm, the method comprises the following steps: carrying out classification and standardization processing on animal quarantine data to obtain standard animal quarantine data, inputting the standard animal quarantine data into a pre-constructed animal quarantine model, outputting an animal quarantine feature tree, and carrying out hierarchical storage on the standard animal quarantine data and the animal quarantine feature tree to obtain an animal quarantine database. The method can realize efficient storage of animal quarantine data.

The invention provides an isothermal amplification primer probe group, a rapid detection kit, a method and application for detecting Xanthomonas vesicans of Carya illinoensis, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current carya illinoensis scab is difficult to identify, excessively depends on expensive instruments and technicians and the like, the research on the LFD-RPA detection technology of the carya illinoensis scab is developed, and the carya illinoensis scab can be simply, conveniently and efficiently detected; in addition, the kit has no cross reaction with 18 representative strains of other species and similar species (cladosporium) and other 14 genus in the genus of furazolospora, the specificity is strong, the lowest detection limit is 5 pg genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

The invention discloses animal husbandry veterinary quarantine detection equipment, and relates to the technical field of quarantine detection. The animal husbandry veterinary quarantine detection equipment comprises a placing seat, a fixing frame is assembled at the top of the placing seat, a power rod driven by an electric push rod is assembled at the bottom of the fixing frame, and a blood sampling tube body is assembled at the bottom of the power rod. According to the animal husbandry and veterinary quarantine detection equipment, an animal is placed on a placement plate in a placement seat, an electric telescopic rod is started, and a movable rod, an arc-shaped outer plate, an elastic telescopic rod, an arc-shaped inner plate, a hydraulic bin I, a stress rod, a push plate, a spring I, a spring II and a pressing plate are matched, so that when blood sampling operation is not performed yet, only the animal is preliminarily fixed, and the blood sampling operation is performed; when blood sampling operation is carried out, the animal is further fixed, and after the blood sampling operation is completed, the animal is quickly restored to a preliminary fixing state, so that the clamping time is shortened, and the possibility of injury to the animal is reduced.

The invention provides a device and method for efficient inspection and quarantine of beef and mutton, and relates to the technical field of meat inspection and quarantine. The device comprises a base, a placing plate is fixed on the top of one side of the base, and a detection platform is fixed on the top of the other side of the base, a detection device is installed on the upper end of the base located at the detection platform, a telescopic frame is fixed on the back of the placing plate of the base, and a top plate is fixed on the top extended end of the telescopic frame, a first screw is threadedly inserted on the surface of the storage end of the telescopic frame, and the top of the first screw is rotatably connected to the extended end of the telescopic frame through a bearing seat, and the cooperation of a collection tube assembly, a driving assembly and a cutting assembly facilitates the penetration of a certain depth into the meat, and then the meat blocks are taken out, and the blocks are cut and sent out separately according to the positions to be taken out, and are collected by a collection box, which is convenient for subsequent inspection and quarantine, improves the collection of beef and mutton blocks at different positions and different depths, and improves the collection and subsequent inspection and quarantine efficiency.

The invention relates to the field of immunology, and discloses a polypeptide and a kit for distinguishing bovine and sheep brucella natural infection from a vaccine immune antibody. Polypeptides as shown in SEQ ID NO: 1 and SEQ ID NO: 2 are artificially synthesized and used as antigens to coat an elisa plate and a nitrocellulose membrane, and according to response characteristics of antibody level, identification and detection of natural infection and vaccine immunity of cattle and sheep populations are realized. The peptide fragment and the detection kit provided by the invention can make up the blank of identifying bovine and sheep brucella natural infection and immune antibodies. The method solves the problem that the antibody induced by the attenuated live vaccine and the naturally infected antibody are difficult to distinguish, provides a scientific basis for quarantine and purification of Brucella, and provides a technical guarantee for preventing human diseases and animals, advancing the mouth and protecting the body health of people.

The invention provides an ecological breeding device for mosquito breeding, and relates to the field of biological breeding for biological quarantine. A breeding water tank is fixedly connected to the right part of the top end surface of the fixed bottom plate; a larva grid box is arranged in the breeding water tank; the top end surface of the larva grid box is fixedly connected with a first telescopic box; the top end surface of the first telescopic box is fixedly connected with an eclosion box; a first mosquito-lured lamp is arranged in the eclosion box; the left part of the top end surface of the fixed bottom plate is fixedly connected with a fixed base; the top end face of the fixed base is fixedly connected with a feeding box. A connecting frame is pulled, an insect separation baffle is closed through a series of transmission, an adult box is separated from a feeding box, after the insect separation baffle is closed, a cleaning brush can be driven to move along with continuous movement of the connecting frame to sweep fallen dead mosquitoes, and the problems that space in the adult box is generally compact and narrow, and the adult box is inconvenient to clean are solved. And an experimenter is inconvenient to clean the mosquito corpses in the corners.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention discloses a rapid detection and intelligent early warning system for port concerned infectious diseases, and relates to the field of customs quarantine, and the system comprises a target selection unit, a construction unit, a modification unit, an amplification unit, a purification unit, a determination unit and a detection unit. According to the invention, through systematic improvement in the aspects of multi-primer design and modification, amplification system optimization, product purification, multi-dimensional scoring decision and the like, many defects in the aspects of multi-pathogen detection, dimer interference suppression, detection efficiency and sensitivity improvement in the prior art are overcome; rapid, efficient and intelligent screening and real-time early warning of port concerned infectious diseases are realized.

The invention discloses a quadruple fluorescent quantitative PCR (Polymerase Chain Reaction) primer probe group for rapidly detecting four pathogens of birds and application, and belongs to the technical field of biology. The kit comprises specific primers and probes aiming at coronavirus (gamma, delta), avian influenza virus (H9 subtype), Newcastle disease virus and West Nile virus; the reagent disclosed by the invention is used for detecting four viruses carried by birds, and has the advantages of high specificity, strong sensitivity, short detection time, safety, low cost and the like; the method can realize detection of pathogens carried by birds, and is of great significance to customs frontier defense and quarantine work.

The utility model belongs to the technical field of animal husbandry quarantine, and discloses animal husbandry veterinary quarantine equipment which comprises a base, two fixing mechanisms are arranged on the outer wall of the top end of the base, a clamping mechanism is arranged on the inner wall of the base and comprises a motor, an output shaft of the motor is fixedly connected with a gear A, and the output shaft of the motor is fixedly connected with a gear B; a rotating plate is rotationally connected to the inner wall of the top end of the base, a gear B is fixedly connected to the outer wall of the bottom end of the rotating plate, and multiple sets of arc-shaped grooves are formed in the outer wall of the rotating plate. A motor is started to drive a gear A to rotate and can drive four sets of gears B meshed with the gear A to rotate at the same time, so that four sets of rotating plates can be driven to rotate, clamping plates on each set of rotating plates can move towards the middle at the same time to clamp the four limbs of livestock, the situation that the quarantine effect is affected by walking in the quarantine process of the livestock is avoided, and therefore the stability in the quarantine process of the livestock is improved; and meanwhile, the operation of clamping the four limbs is more convenient and faster.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The invention discloses a sampling method for veterinary quarantine, a transfer cylinder is slidably arranged in a sampling cylinder, and the invention relates to the technical field of sampling quarantine. According to the sampling method for veterinary quarantine, a sampling mechanism, a positioning mechanism and an automatic reset mechanism are arranged among a sampling cylinder, a transfer cylinder, a spiral conveying shaft, an adjusting cylinder and a long sampling cylinder, so that the sampling mechanism, the positioning mechanism and the automatic reset mechanism can be synchronously cooperated when the device is used; on the basis that the outer layer of excrement is automatically pushed aside, the device can achieve automatic sampling, pungent smell evacuation, sampling convenience improvement and device use comfort level improvement, the holding position of the handle ring sleeve and the pressing position of the handle ring sleeve on the control button are synchronously and automatically limited, additional force application is reduced, and the labor intensity is reduced. And after the sampling is completed, the automatic resetting mechanism is used for automatically resetting the telescopic state of the sampling mechanism and the position control of the positioning mechanism.

According to the whole-process traffic scheduling trajectory tracking method and system based on the traffic scheduling bracelet, the contact distance between a diagnosed case and a close contact person is accurately calculated through the signal intensity of the WiFi equipment and the Bluetooth equipment around the traffic scheduling bracelet by using the monitoring mode of the traffic scheduling bracelet, so that the traffic scheduling close contact information table of the diagnosed case is obtained, and the traffic scheduling trajectory tracking accuracy is improved. According to the invention, automatic collection and accurate matching of the close-connection information of related personnel such as doctor seeing personnel, medical personnel, hospitalized personnel, accompanying personnel, isolation personnel, management personnel, working personnel and auxiliary personnel in key places such as infectious disease hospitals, shelter hospitals or isolation hotels in the epidemic prevention and control period can be realized at low cost, so that the close-connection efficiency is improved; in addition, uncertain factors caused by time hysteresis are greatly reduced, the accuracy of close-packed results can be obviously improved, and the pressure in epidemic prevention and control work is greatly relieved.

The utility model discloses a livestock quarantine excrement sampling device and belongs to the field of excrement sampling. The animal husbandry quarantine excrement sampling device comprises a fixed pipe and further comprises a plurality of flexible rods which are fixedly connected to the lower end of the fixed pipe at equal intervals in a circumferential mode; the fixed rod is arranged on the lower side of the flexible rod; the disposable viscose cloth is arranged on the lower sides of the fixing rods; the utility model effectively solves the problem that the dilute excrement is inconvenient to sample at present, effectively improves the sampling efficiency of the dilute excrement, and simultaneously effectively prevents the cotton swab from being in contact with the ground to be stained with excrement of other pigs when the cotton swab is used for sampling.

The invention discloses a machine vision device for multilayer sorting and quarantine of harmful organisms of imported bulk grains, and belongs to the technical field of multilayer sorting and quarantine of harmful organisms of imported bulk grains, the machine vision device is provided with a hopper convenient to position and assemble, the lower surface of the hopper is connected with a first-stage screening part, and the rear side of the lower surface of the first-stage screening part is provided with a first vibration part; comprising a first conveying belt located on the middle rear side of the lower surface of a first-stage screening part, a first supporting frame is rotationally connected to the outer surface of the first conveying belt, a first material receiving part is supported at the left end of the upper surface of the first supporting frame, and meanwhile a second-stage screening part is arranged at the front end of the lower surface of the first-stage screening part. In the sampling process, screening, quarantine, sorting and packaging are completed in parallel, the sample detection quantity, detection efficiency and detection consistency are greatly improved, the detection time is shortened, samples are divided into three layers according to the particle size, the image acquisition effect of an industrial camera in the industry is guaranteed, and then the detection precision is improved. And the clamping structure is matched to control the stability of the industrial camera.

The invention discloses a loop-mediated isothermal amplification detection primer group for Rhodospora pini Rhodospora acuminata and an application of the loop-mediated isothermal amplification detection primer group for Rhodospora pini Rhodospora acuminata. The invention designs four groups of loop-mediated isothermal amplification detection primer groups of Rhodospora pini Maxillaris, which are respectively a primer combination T1, a primer combination R1, a primer combination R3 or a primer combination R5, of Rhodospora pini Maxillaris. Experimental results show that the loop-mediated isothermal amplification detection primer group and the loop-mediated isothermal amplification detection method of the pine needle red spot pathogen botrytis aculeatus by using the loop-mediated isothermal amplification detection primer group have the characteristics of high specificity, low requirements on instruments and equipment, convenience in operation and the like; in production practice, rapid, sensitive, accurate, safe and visual detection of the pine needle red spot pathogen can be achieved, the method can be used for field investigation and early diagnosis of the pine needle red spot pathogen, quarantine and transportation of nursery stocks and disease monitoring and identification, and reliable technical and theoretical bases are provided for early warning and prevention and control of the pine needle red spot pathogen.

The invention provides a method for constructing an animal model with sicca syndrome combined with pulmonary fibrosis, and relates to the technical field of animal model construction.The method comprises the following steps that 8-week-old NOD / Ltj mice qualified in quarantine are purchased and fed for 12 weeks; in 12 weeks, bleomycin (BLM) is injected into the mouse in the trachea, the injection dosage of bleomycin is 3 mg / kg, and construction of the sicca syndrome combined pulmonary fibrosis animal model is completed 21 days after injection. The SS-PF mouse model disclosed by the invention has high repeatability and stability, can be constructed on a large scale, and is suitable for various research requirements. The model is not only suitable for basic research, but also can be applied to drug screening and curative effect evaluation, and has a wide application prospect. Through the model, large-scale drug screening experiments can be carried out, the effectiveness and safety of drugs can be evaluated, a new treatment choice is provided for SS-PF patients, and the clinical treatment effect is improved.

The present invention relates to a movable port quarantine cabin, the purpose of which is to provide a flexible, well-insulated, effectively partitioned workplace for customs port quarantine operations to prevent cross-contamination. It is equipped with a trailer chassis and a body, which can move in the form of a vehicle and also provide a quarantine office space in the form of a house. The body is provided with side walls and a top cover, and the side walls are provided with doors. Inside the body, functional rooms such as a workroom, a sampling room, a disinfection room, a dressing room, etc. and an equipment room are effectively partitioned by wall panels. Disinfection devices and air change devices are provided in the functional rooms, and the layout of the functional rooms can prevent cross-contamination. The present invention can be widely applied to occasions where outdoor quarantine operations are carried out at customs ports.

The invention belongs to the technical field of plant leaf punching and sampling devices, particularly relates to a puncher for accurately detecting nucleic acid of plant leaves and a use method, and aims to solve the problems that existing sampling devices are all provided with metal modules and are reusable sampling devices, and single sampling device is large in size and inconvenient to use. In order to solve the problem that in the prior art, in the prior art, in the prior art, the use requirements of portability and disposable use required by plant leaf pathogen collection are difficult to meet, the following scheme is provided: the device comprises a punching cylinder; the first limiting hole and the second limiting hole are formed in the outer side of the punching barrel; according to the present invention, during the use process, the portable rapid pathogen detection device has characteristics of portability, easy installation, simple sampling operation, convenient and rapid sampling process, consistent size of the cut leaf tissue, regular and uniform shape, and increase of the accuracy of the pathogen detection result, and can be used for the field rapid detection of the field plant, the method has important significance on quarantine, detection, monitoring and prevention and control of plant diseases and insect pests.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

The present invention discloses a primer set and a detection method thereof for synchronously detecting a plurality of quarantine pathogens of soybean. The primer set is composed of a detection primer pair SoqF / SoqR for Phytophthora sojae, a detection primer pair DPM1F / DPM1R for southern soybean stem canker pathogen, a detection primer pair DPC2F / DPC2R for northern soybean stem canker pathogen, and a detection primer pair FV3F / FV3R for North American soybean sudden death syndrome pathogen. The primer set is added to the same PCR reaction system, and specific amplification products of different sizes are obtained by multiple PCR amplification and agarose gel electrophoresis. The primer set and the detection method of the present invention have strong specificity and high sensitivity, and can quickly and accurately synchronously detect a plurality of quarantine pathogens in soybean in a system. The primer set can be used for port inspection and quarantine, early diagnosis of field diseases, and monitoring and identification of pathogens.

The application discloses a reagent, a method and application of the reagent and the method for simultaneously detecting three common viruses in a fancy carp, and belongs to the technical field of fish quarantine. The application provides a reaction system and a detection method for simultaneously detecting three common viruses in a fancy carp, establishes a multiplex fluorescence PCR method, adopts a full-closed reaction, solves the problem that common PCR is prone to generating aerosols and causing false positive results of tests, has high detection sensitivity, can detect target genes with a copy number of 10 orders of magnitude at the minimum, is suitable for detecting a small amount of viruses carried in normal fancy carp or viruses in water quality environment, and can fully meet the requirements of customs quarantine prevention and control, has strong specificity, has no cross reaction to templates such as IHNV, RSIV and EHNV, and further provides a virus-like particle which can be used as a positive reference product in the multiplex system, is stable in state and beneficial to storage, can monitor an extraction process, and ensures that a test is established.

The application discloses a waste stratified sealing and recycling device for health quarantine, which comprises a rail frame vertically fixed on a base plate, a discharging device arranged on the side of the rail frame, the discharging device being capable of reciprocating lifting along the extension direction of the rail frame, a treatment bin fixed on the upper part of the other side of the rail frame, a positioning and sealing assembly arranged at the lower end of the treatment bin, the positioning and sealing assembly being capable of sealing, packing and collecting the treated quarantine waste, and the discharging device being capable of fixing a garbage can and pouring the quarantine waste in the garbage can into the treatment bin.