29 results about "Quarantine" patented technology

This utility model discloses a port risk management entry-exit quarantine device, including a storage box with several evenly distributed rollers at the bottom. A mounting plate is located on one side of the storage box, connected to the storage box via a connecting frame. Symmetrically arranged movable plates are located at the top of the storage box, and two sets of movable plates are connected to the mounting plate via a movable component. Beneficial effects: By setting the movable component, the two sets of movable plates can be adjusted to move synchronously relative to each other, allowing animal limbs to be quickly placed between clamping plates one and two. The movable component two can move clamping plates two closer to clamping plates one until the animal limbs are clamped tightly. The movable component two uses a screw rod two to engage with a movable connecting column for secure clamping. Both movable components one and two are driven by motors, eliminating the need for manual operation and saving time and effort.

The application discloses a pathogenic microorganism whole genome tracing library construction method and system for port quarantine, and belongs to the field of biological information and big data processing, which comprises the following steps: obtaining whole genome sequences and context data, generating knowledge units containing context enhancement and virtual variation fingerprints through a local trend prediction model, and mapping the knowledge units to a dynamic tracing knowledge graph; submitting the virtual variation fingerprints to a global mirror knowledge space for collaborative rehearsal to identify risk signals; carrying out directional depth analysis and updating the graph; submitting an encrypted knowledge abstract to the global space to drive consensus evolution; and optimizing the prediction model and identification rules based on the consensus results. The closed-loop mechanism combining local and global collaboration and virtual and real verification can realize active discovery of variation pathogens and automatic evolution of the knowledge base, and improve the rapid tracing and early warning capabilities of the port for sudden epidemics.

This invention discloses a multimodal image fusion method and system for monitoring pig health, belonging to the field of pig farming and animal quarantine technology. It achieves real-time detection through synchronous acquisition and spatiotemporal alignment of multimodal images, preprocessing and optimization, attention mechanism feature fusion, and spatiotemporal Transformer health status recognition, combined with an edge-center collaborative architecture. A self-optimizing closed loop is constructed through manual review and incremental training. The system is equipped with a multi-module collaborative architecture, with each module implementing image acquisition, preprocessing, feature fusion, status recognition, real-time detection, and model optimization functions. This invention integrates multimodal image information and jointly models spatiotemporal features, significantly improving detection accuracy and efficiency. It features early warning, anti-interference, and self-learning evolution, making it suitable for automated pig entry quarantine lines in large-scale farms and slaughterhouses, providing technical support for intelligent livestock disease prevention and control.

The application discloses a special temporary storage box for mollusk scanning, and relates to the field of biological quarantine imaging auxiliary equipment.The box body is provided with a plurality of supporting feet on the lower end face; a sealing door is installed on one side of the box body; a control bin is fixed to the side wall of the box body, and the inside of the control bin at least comprises a life maintaining system for delivering constant-temperature and constant-humidity gas or circulating water to the inside of the box body and storing anesthetic medium; a restraint assembly is arranged in the inside of the box body; and an inner lining assembly is fixed in the inside of the box body and located below the restraint assembly.

This invention relates to the quantitative detection of pigeon adenovirus and the simultaneous differential diagnosis of type I and type II. Specific primers and probes for pigeon adenovirus type I and II are designed using specific gene sequences. Real-time quantitative PCR can accurately detect and differentiate between pigeon adenovirus types I and II. Verification has shown that this technology can rapidly and accurately differentiate between PiAdV-I and PiAdV-II, exhibiting high specificity and sensitivity. Furthermore, it is cost-effective, easy to operate, and suitable for import / export quarantine and specialized laboratories for the detection and differentiation of PiAdV-I and PiAdV-II.

The application discloses an RPA-CRISPR Cas12a rapid visual detection system for Cercospora apii and application thereof, and relates to the technical field of plant pathogenic microorganism molecular detection. The system comprises an RPA primer pair, crRNA, Cas12a protein and a single-stranded DNA reporter molecule designed for a TUB2 gene. The detection method is as follows: sample DNA is extracted, and is subjected to RPA constant-temperature amplification for 20 minutes; the product is added into a CRISPR / Cas12a system, and is incubated at 38 DEG C for 10 minutes; and the result is judged through a fluorescence signal or a test strip. The application has a sensitivity of 34 ag / muL, can complete detection within 80 minutes, has strong specificity, does not need complex instruments, is suitable for port quarantine, field monitoring and seed health detection, and has good practicability and popularization value.

This invention discloses primer-probe combinations, kits, and applications for the dual-system detection of TobRFV (Tobacco Brown Fruit Virus) using TaqMan and EmDEA, belonging to the field of viral molecular diagnostics technology. Based on highly conserved and specific viral core target sequences selected through screening, this invention constructs primer-probe combinations suitable for both TaqMan qPCR and EmDEA detection systems. These combinations target two different genes of ToBRFV (p126 and MP), not only specifically detecting ToBRFV and exhibiting no cross-reactivity with viruses of the same genus such as TMV, ToMV, and ToMMV, but also broadly covering the vast majority of ToBRFV isolates. This invention effectively reduces the risk of missed detection in single-target detection and has significant practical application value for accurate quantification of this virus in the laboratory and rapid field quarantine.

The present application relates to the technical field of biological detection, and a double-path apple codling moth identification system integrating RPA-LFS rapid screening and TaqMan qPCR real-time quantification, a locked nucleic acid probe primer set, a kit and a method thereof. COI Through gene screening and primer screening, the present application designs recombinase-mediated isothermal amplification (RPA) and TaqMan qPCR primers and probes for the barcode of the cytochrome oxidase subunit I (COI) gene of apple codling moth, and establishes an RPA-LFS method in combination with immunolateral flow. The RPA-LFS method can complete amplification and visual judgment within 20 minutes at 39 DEG C, and is suitable for on-site rapid detection. The TaqMan qPCR method can realize high-sensitivity quantitative analysis under the condition of crude extract. Through double-path complementary design, the consistency of the results of on-site screening and laboratory confirmation detection is realized, and the method is simple to operate and suitable for pest quarantine at ports, epidemic monitoring in agricultural production areas and quarantine at storage and transportation links.

This utility model discloses a cleaning device for silkworm mother moth inspection equipment, belonging to the field of silkworm moth quarantine technology. The device includes: a box with mounting platforms on both sides and a funnel-shaped structure with a drain outlet at the bottom; a side spray washing section mounted on the mounting platforms, forming a cleaning channel in the middle, with spray nozzles facing the cleaning channel; one end of the cleaning channel is the inlet for the inspection equipment, and the other end is the outlet; a bottom cleaning pipe located below the cleaning channel, providing sliding support for the inspection equipment placed upside down in the cleaning channel; cleaning holes on the top surface of the bottom cleaning pipe spray water upwards to rinse the inside of the inspection equipment; and a top cleaning section covering the top of the cleaning channel, sealing the top of the cleaning channel, with downward-facing cleaning nozzles spraying water to rinse the inspection equipment. This utility model can accurately and efficiently rinse away sample mother moth grinding residue or other chemicals from inspection equipment such as funnels and cups.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination that specifically recognizes the hemagglutinin protein of the H6 subtype of avian influenza virus and its applications. The antibody combination consists of monoclonal antibodies 2D12 and 4G3, targeting different epitopes of the H6 hemagglutinin protein, and possesses clearly defined heavy and light chain variable region complementarity-determining region sequences, as shown in SEQ ID NO. 1-12, respectively. The antibody combination exhibits high specificity and affinity, effectively avoiding cross-reactivity with other influenza subtypes such as H1, H5, H7, and H9, as well as common avian pathogens. The colloidal gold test strip constructed based on this antibody combination is highly sensitive and easy to operate, suitable for in vitro detection and epidemiological screening of the H6 subtype of avian influenza virus. It solves the false positive problem caused by cross-reactivity in existing technologies, providing a reliable technical means for the monitoring and quarantine of the H6 subtype of virus.

1. The name of the design product: high pruning shears (forestry pest quarantine control). 2. The use of the design product: high pruning shears for pruning tree and plant branches. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

This invention discloses a primer set, reagent kit, and detection method for detecting Mediterranean fruit flies, belonging to the field of bioquarantine and molecular detection technology. This invention designs a dedicated primer set targeting specific gene segments of the Mediterranean fruit fly, including DNA upstream primers, DNA downstream primers, and RNA primers, and establishes a standardized stepwise primer screening strategy. This strategy sequentially performs pressure screening of RNA primers, DNA downstream primers, and DNA upstream primers, using Ct value, endpoint fluorescence value, and template-free control results as stringent evaluation indicators to quickly identify the optimal primer combination. Furthermore, this invention provides an optimized mix system and a 42℃ isothermal amplification detection procedure. This invention effectively overcomes the shortcomings of existing technologies, such as low sensitivity and easy cross-reactivity, achieving a detection limit as low as 5 copies / μl, high specificity, and detection completed in 30 minutes, making it extremely suitable for rapid applications in port quarantine and field monitoring.

The application discloses a triplex forming oligonucleotide fluorescent probe and a detection method for detecting mycoplasma by targeting P1 adhesion protein genes, and belongs to the technical field of microbial molecular detection. The fluorescent probe is a triplex forming oligonucleotide (TFO), targets a specific conservative sequence of mycoplasma P1 adhesion protein genes, the 5' end of the fluorescent probe is modified with a fluorescent reporter group, the 3' end of the fluorescent probe is modified with a fluorescent quenching group, the fluorescent probe can form a stable triplex DNA complex with the target sequence through Hoogsteen base pairing and generate a fluorescent signal. The detection method comprises four core steps of sample pretreatment and DNA extraction, fluorescent probe incubation, fluorescent signal detection and result judgment, and does not need PCR amplification, and is simple in operation and rapid in detection. The probe has high specificity and high sensitivity, the detection lower limit can reach 1 copy / muL, is suitable for mycoplasma detection of various types of samples, and can be widely applied to scenes such as clinical diagnosis, biological pharmaceutical quality control and livestock breeding quarantine.

The present application relates to the technical field of beef slaughtering quarantine traceability, in particular to a beef product slaughtering quarantine information intelligent traceability system based on big data, comprising: collecting target cattle whole-process slaughtering data through a data acquisition module to construct a quarantine state transition diagram from individual cattle to specific meat products; a risk assessment module quantifies the quarantine risk transmission probability of each link according to the above to generate an individual quarantine risk assessment spectrum; a data fusion module integrates cattle historical breeding, circulation and risk assessment data to form a multi-dimensional quarantine correlation knowledge graph; a path analysis module identifies risk aggregation paths and key control points through community discovery and path analysis; and a traceability report module reversely simulates risk propagation to code and generate machine-readable electronic traceability reports. The system breaks down the data isolation barriers of each link, realizes accurate traceability and intelligent control of beef slaughtering quarantine risks, and improves the accuracy and intelligent efficiency of quarantine traceability.

The present application relates to the technical field of identification coding, and in particular to an electronic quarantine identification traceability management system. The present application ensures the authenticity and reliability of the data source by obtaining the birth date and immunization date of animals; calculates the age difference of adjacent individuals and immunization, combines historical data to dynamically construct batch consistency mean and dispersion, and realizes the quantitative characterization of the batch biological attribute consistency; uses attribute outlier coefficient to quantify the standardized deviation of individuals relative to the normal level of the batch, and introduces the scanning time for correction, which unifies the physical operation rate and the biological attribute deviation into a dimensionless operation abnormality evaluation index; the simulator extremely fast single factor is amplified due to the scanning time approaching zero, and the heterogeneous individual mixing factor is directly reflected due to the increase of the attribute outlier coefficient, which solves the two types of violation problems without increasing the hardware cost; finally, the reliability and accuracy of the electronic quarantine identification traceability are significantly improved through the index.

The utility model relates to an assembly type mobile animal house, including function layer and equipment layer, the functional layer is divided into a plurality of compartments in the part, and the compartment includes preparation room, changing room, buffer room, clean walkway, operating room, feeding room, quarantine room and retreat buffer room, and the preparation room is communicated with the clean walkway through changing room and buffer room in proper order, and the clean walkway is communicated with the preparation room through retreat buffer room, and the quarantine room is communicated with the clean walkway, and the clean walkway is communicated with the feeding room through operating room, and the door for cutting off adjacent compartments is arranged between adjacent compartments, and the first delivery window is arranged between the preparation room and the feeding room, and the second delivery window is arranged between the preparation room and the quarantine room, and the equipment layer includes air supply air conditioner box and exhaust fan unit, and the air supply pipe extending to each compartment is connected on the air supply air conditioner box, and the exhaust pipe extending out from each compartment is connected on the exhaust fan unit. The utility model adopts the design of integrated type, not only reduces the overall volume of animal house, and construction installation is very convenient.

This utility model discloses a livestock and veterinary quarantine and testing device, belonging to the field of livestock and veterinary quarantine technology. It includes a base plate and a testing instrument mounted on the base plate. A connecting component is mounted on the testing instrument, and a testing cylinder is mounted on the connecting component. A rotating block is mounted on the base plate, and a placement frame is rotatably mounted on the rotating block. The placement frame has rotating holes that cooperate with the rotating block. A guide component is mounted on the base plate, and a limiting component is slidably mounted on the guide component. A support component is mounted on the placement frame, and multiple test tubes are mounted on the placement frame. This utility model, through the rotatable connection between the slot and the rotating hole, facilitates the rotation of the placement frame and adjustment of the position of the test tubes on the placement frame, making it easy for the user to pick up the test tubes. The limiting frame and support rod, working together, effectively limit the position of the test tubes, preventing them from tipping over.

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for specifically recognizing hemagglutinin protein of avian influenza virus H6 subtype and a use thereof. The antibody combination is composed of monoclonal antibodies 2D12 and 4G3, is directed to different epitopes of the H6 hemagglutinin protein, has clear heavy chain and light chain variable region complementarity determining region sequences, and is respectively shown as SEQ ID NO. 1-12. The antibody combination has high specificity and affinity, and can effectively avoid cross reactions of other influenza subtypes such as H1, H5, H7 and H9 and common avian pathogens. A colloidal gold detection test strip based on the antibody combination has high sensitivity and simple operation, is suitable for in vitro detection and epidemiological screening of the H6 subtype avian influenza virus, solves the false positive problem caused by cross reactions in the prior art, and provides a reliable technical means for monitoring and quarantine of the H6 subtype virus.

The utility model provides a kind of meat duck slaughtering and processing is used to inspect quarantine device, it is related to meat duck slaughtering and processing technical field, the meat duck slaughtering and processing is used to inspect quarantine device, including base, the side fixed mounting of base is used to attract meat duck's trough, the side fixed mounting of base upper surface close to trough is equipped with mounting seat, the both ends of mounting seat are fixedly installed with fixed block, the inboard of mounting seat and fixed block are fixedly installed with first fixing device for fixing duck foot, the inside of the through groove is equipped with second fixing device for fixing duck neck;By setting trough on the side of base, and setting mounting seat on its upper surface close to trough, and setting first fixing device on the side of mounting seat to fix duck foot, and setting through groove and limiting slot in mounting seat to install second fixing device to fix duck neck, to reach the purpose of convenient inspection and quarantine.

The utility model relates to color selection device technical field, concretely is a kind of small soybean color selection device, including shell, first pulse air valve and second pulse air valve are arranged in the shell, and two are staggered spatial layout, the surface of the shell is provided with first discharge channel, the first pulse air valve is used to spray ordinary impurities to first discharge channel, the surface of the shell is provided with second discharge channel, the second pulse air valve is used to spray target risk object to second discharge channel, the surface of the shell is provided with third discharge channel, the utility model's purpose is to solve when in customs quarantine, target risk object needs to be strictly isolated and needs to be destroyed according to biological safety process, traditional color selection machine uses single -way waste collection design, two kinds of substances need artificial secondary sorting after mixing to carry out compliance processing to target risk object, increase the problem of operation complexity.

The utility model relates to the technical field of microbiological quarantine, and disclose a kind of culture device for fruit and vegetable microbiological quarantine, including incubator, the inside of the incubator is provided with fixed mechanism, the front of the incubator is hinged with box door by hinge, the fixed mechanism includes placement platform and two-way screw rod, and the two-way screw rod is rotatably connected in the inside of placement platform by bearing. The utility model places the middle part of placement platform to petri dish, and staff can rotate handle to drive two-way screw rod to rotate, so that the spacing between its first adjusting frame and second adjusting frame can be adjusted, so that its first pressing plate and second pressing plate are moved to the edge upper surface of petri dish, then start first cylinder and second cylinder to make two pressing plates press on petri dish, limit and fix petri dish, it is convenient to fix different size petri dish, and improve the application range of culture device.

The utility model discloses an isolation room for entry and exit animal inspection and quarantine, every isolation room is equipped with animal excrement cleaning mechanism, and the animal excrement cleaning mechanism includes the hollow board of setting in the bottom of isolation room, is equipped with the excrement collection surface of the inclined of setting under the hollow board, and the low end of excrement collection surface is equipped with the excrement collection groove, and the bottom surface inclination direction of excrement collection groove is perpendicular to the inclination direction of excrement collection surface, and the side of the multiple isolation rooms of setting in a row is equipped with the excrement collection ditch, and the bottom surface inclination direction of excrement collection ditch is perpendicular to the bottom surface inclination direction of excrement collection groove, and the low end of the bottom surface of excrement collection ditch is equipped with the excrement collection pool, and the low end of the bottom surface of each isolation room excrement collection groove is equipped with the discharge port of being linked together with excrement collection ditch, and the discharge port is equipped with the discharge valve, and the high end of excrement collection surface is equipped with the disinfectant high pressure spout mechanism, and the high end of the bottom surface of excrement collection groove and the high end of the bottom surface of excrement collection ditch are equipped with the water high pressure spout mechanism.

This invention discloses a TaqMan real-time fluorescent PCR detection primer and probe set, kit, and detection method for Cuscuta pentaphyllum. The primer and probe set includes: an upstream primer C. pan F, whose nucleotide sequence is shown in SEQ ID NO.1; a downstream primer C. pan R, whose nucleotide sequence is shown in SEQ ID NO.2; and a TaqMan probe C. pan P, whose nucleotide sequence is shown in SEQ ID NO.3. The primers and probe target specific fragments of the rbcL gene sequence of Cuscuta pentaphyllum, achieving specific recognition of Cuscuta pentaphyllum through sequence complementarity matching. This avoids false positive results caused by cross-reactions with closely related species and host plants at the molecular targeting level, effectively solving the problem of difficulty in distinguishing closely related species in traditional morphological identification. Compared with traditional morphological identification and conventional PCR, this invention also features high detection efficiency, short processing time, simple operation, and wide applicability. It is suitable for precise identification in professional laboratories and can also meet the practical applications in grassroots quarantine and field monitoring scenarios.

The utility model relates to a live pig isolation quarantine fence with disinfection channel belongs to live pig isolation quarantine fence technical field. The live pig isolation quarantine fence with disinfection channel, include: quarantine fence main part, one end fixedly connected with connecting plate of quarantine fence main part, disinfection mechanism can carry out effective disinfection to live pig isolation quarantine fence and set up in the top of connecting plate, the connecting plate of quarantine fence main part and intercepting rod cooperation constitute live pig disinfection channel, the conveying pipe on intercepting rod can convey potassium peroxymonosulfate disinfectant to water mist spray head, and the water mist is sprayed through water mist spray head and is sterilized to live pig and disinfection channel, and the bacteria are killed, and the first ultraviolet disinfection lamp and second ultraviolet disinfection lamp on the intercepting rod and quarantine fence main part emit ultraviolet light in advance and disinfect the intercepting rod and connecting plate and quarantine fence main part, can carry out effective disinfection sterilization to live pig isolation quarantine fence, prevent live pig cross infection.

The application discloses a probe primer group, a kit and a detection method for detecting European dodder, wherein the probe primer group comprises an upstream primer Ce-rbcL-F, a downstream primer Ce-rbcL-R and a TaqMan probe Ce-rbcL-P; the nucleotide sequence of the upstream primer Ce-rbcL-F is shown as SEQ ID NO. 1; the nucleotide sequence of the downstream primer Ce-rbcL-R is shown as SEQ ID NO. 2; and the nucleotide sequence of the TaqMan probe Ce-rbcL-P is shown as SEQ ID NO. 3. The primer probe group is designed based on a specific region of the rbcL gene of European dodder, and specific identification of European dodder can be realized by combining an optimized real-time fluorescent PCR reaction system and conditions, namely, the application has the characteristics of rapidness, strong specificity, high identification accuracy, effectively solves the problem that near relatives are difficult to distinguish in traditional morphological identification, and avoids false positive misjudgment. Compared with traditional morphological identification and conventional PCR, the application also has the characteristics of high detection efficiency, short time consumption, simple operation and wide applicability, and can be applied to not only professional laboratory accurate identification, but also practical application of scenarios such as primary quarantine and field monitoring.

The utility model discloses a biological safety constant temperature and humidity bird quarantine device with dirt recovery function, include: bird quarantine ware body, its inside is provided with the cavity, and the cavity bottom surface central part is provided with the blow-off port, and the blow-off port is provided with the guide inclined plane between the cavity lateral wall, and the blow-off port top is provided with the net rack, its fixed mounting is at the blow-off port, and the blow-off pipe includes the gathering section and the temporary storage section that are arranged in sequence from top to bottom, and the gathering section is the taper structure of wide upper narrow lower, and the gathering section is provided with the several shower nozzles of circumferential arrangement, and the shower nozzle is used for the shower liquid to the gathering section inner wall, and the temporary storage section is provided with the baffle, and the baffle rotatable mounting is in the blow-off pipe, and the pivot of baffle connects drive motor output, and the temporary storage section is provided with the liquid level sensor, and the liquid level sensor sets up in the baffle top. Compared with prior art, the utility model solves the problem that the excrement of the bird in the bird quarantine ware cannot be collected in time and quantitatively.

This invention belongs to the field of molecular detection technology for crop viruses, and discloses a kit and method for detecting three viruses in cucumber based on droplet digital PCR. This invention provides a composition for the simultaneous detection of multiple viruses in cucumber, namely, Chili Vein Mottle Virus (ChiVMV), Cucumber Green Mottle Mosaic Virus (CGGMV), and Watermelon Silver Mottle Virus (WSMoV). The composition includes specific primer pairs and fluorescent probes designed for multiple viruses, with sequences shown in SEQ ID NO:1-9. The technical solution of this invention achieves a detection sensitivity of 1 copy / μL, a repeatability coefficient of variation of <5%, and an overall detection cycle of only 6-8 hours. It can effectively detect low concentrations of latent infecting viruses, solving the technical problems of insufficient sensitivity, inability to simultaneously detect multiple target viruses, and inaccurate quantification in existing detection methods. This provides reliable technical support for early warning, precise prevention and control, and quarantine inspection of cucumber viral diseases.

This invention relates to the field of conveying equipment and discloses an automated viscera separation device for simultaneous quarantine during pig slaughter. The device includes a conveying mechanism, a truss, and multiple separation mechanisms. Each separation mechanism includes a tray, a support frame, a rotating frame, a rotating component, and multiple adsorption assemblies. Each adsorption assembly includes a suction head, a first flexible hose, a reversing valve, and a hanging frame. An air chamber is formed within the rotating frame. The reversing valve is a push-button type, with its two inner ports connected to the inner ports of the first flexible hose and the suction head, respectively, and its outer port connected to the outside. The air chamber is connected to an external negative pressure fan. A diversion mechanism is provided on the bottom side of the output end of the conveying mechanism, and a synchronous pushing mechanism is provided on the bottom side of the conveying mechanism. This invention solves the problem that quarantine operators' manual contact with the surface of viscera significantly increases the risk of disease transmission and spread during the slaughtering process.