227 results about "Quarantine" patented technology

The invention discloses a rapid sequencing and traceability analysis system for input infectious diseases, which relates to the field of customs quarantine and comprises a sample preprocessing unit, a high-throughput sequencing unit, a data processing and quality control unit, a rapid comparison and annotation unit and a traceability analysis unit. According to the input infectious disease rapid sequencing and traceability analysis system, a complete link is formed from sample preprocessing, library construction, data cleaning, pathogen recognition and transmission map construction, module splitting and manual intervention are avoided, and tasks can be automatically completed in large-scale and emergency scenes.

The invention discloses an animal quarantine method, and aims to solve the problems of high omission ratio, early warning lagging and lack of environment optimization of the traditional quarantine means. According to the method, multi-dimensional biological signals such as the heart rate, the respiratory rate, the body temperature and the acceleration of an animal are collected in real time through an ear tag type wearable device, and the anomaly detection precision is improved through a self-adaptive sampling mechanism; meanwhile, the environment monitoring module collects the temperature, humidity and ammonia gas concentration of the farm, and dynamically regulates and controls the temperature, humidity and ammonia gas concentration in case of abnormality. And the edge computing node adopts a long-short-term memory network to fuse and analyze data, outputs an epidemic disease risk probability, and optimizes the model through self-learning. The embodiment shows that the method can perform early warning and environment optimization in the incubation period, the quarantine time is shortened to 5-10 minutes per head, the omission ratio is lower than 5%, and an efficient and accurate epidemic disease prevention and control solution is provided for the breeding industry.

The invention relates to the technical field of storage intelligence, and discloses an early warning and allocation system and method for intelligent inventory of inspection and quarantine reagent consumables, and the system comprises a consumption trend extraction module, a demand prediction module, a risk early warning module, an early warning trigger module, a supply feasibility evaluation module and an intelligent allocation module. Performing seasonal decomposition on the historical consumption record to obtain a consumption trend component; periodically superposing the consumption trend component to obtain a demand prediction result; based on the demand prediction result, performing inventory risk research and judgment on the current inventory to obtain an inventory early warning index; when the inventory early warning index exceeds a safety threshold value, inventory early warning information is generated; according to the consumable demand information, carrying out feasibility evaluation on inventory data and logistics constraint conditions of the supply point to obtain a feasible allocation point; generating a deployment scheme according to the safety inventory standard of the feasible deployment point; according to the invention, the perspectiveness and accuracy of early warning and allocation of the intelligent inventory of the inspection and quarantine reagent consumables can be improved.

The invention relates to the technical field of animal quarantine, and discloses an animal quarantine data management method and system, and the method comprises the steps: obtaining original animal quarantine data, carrying out the preprocessing of the original animal quarantine data, obtaining the processed animal quarantine data, and carrying out the processing of the processed animal quarantine data based on a K-means clustering algorithm, the method comprises the following steps: carrying out classification and standardization processing on animal quarantine data to obtain standard animal quarantine data, inputting the standard animal quarantine data into a pre-constructed animal quarantine model, outputting an animal quarantine feature tree, and carrying out hierarchical storage on the standard animal quarantine data and the animal quarantine feature tree to obtain an animal quarantine database. The method can realize efficient storage of animal quarantine data.

The invention discloses animal husbandry veterinary quarantine detection equipment, and relates to the technical field of quarantine detection. The animal husbandry veterinary quarantine detection equipment comprises a placing seat, a fixing frame is assembled at the top of the placing seat, a power rod driven by an electric push rod is assembled at the bottom of the fixing frame, and a blood sampling tube body is assembled at the bottom of the power rod. According to the animal husbandry and veterinary quarantine detection equipment, an animal is placed on a placement plate in a placement seat, an electric telescopic rod is started, and a movable rod, an arc-shaped outer plate, an elastic telescopic rod, an arc-shaped inner plate, a hydraulic bin I, a stress rod, a push plate, a spring I, a spring II and a pressing plate are matched, so that when blood sampling operation is not performed yet, only the animal is preliminarily fixed, and the blood sampling operation is performed; when blood sampling operation is carried out, the animal is further fixed, and after the blood sampling operation is completed, the animal is quickly restored to a preliminary fixing state, so that the clamping time is shortened, and the possibility of injury to the animal is reduced.

The invention relates to the field of immunology, and discloses a polypeptide and a kit for distinguishing bovine and sheep brucella natural infection from a vaccine immune antibody. Polypeptides as shown in SEQ ID NO: 1 and SEQ ID NO: 2 are artificially synthesized and used as antigens to coat an elisa plate and a nitrocellulose membrane, and according to response characteristics of antibody level, identification and detection of natural infection and vaccine immunity of cattle and sheep populations are realized. The peptide fragment and the detection kit provided by the invention can make up the blank of identifying bovine and sheep brucella natural infection and immune antibodies. The method solves the problem that the antibody induced by the attenuated live vaccine and the naturally infected antibody are difficult to distinguish, provides a scientific basis for quarantine and purification of Brucella, and provides a technical guarantee for preventing human diseases and animals, advancing the mouth and protecting the body health of people.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The invention discloses a rapid detection and intelligent early warning system for port concerned infectious diseases, and relates to the field of customs quarantine, and the system comprises a target selection unit, a construction unit, a modification unit, an amplification unit, a purification unit, a determination unit and a detection unit. According to the invention, through systematic improvement in the aspects of multi-primer design and modification, amplification system optimization, product purification, multi-dimensional scoring decision and the like, many defects in the aspects of multi-pathogen detection, dimer interference suppression, detection efficiency and sensitivity improvement in the prior art are overcome; rapid, efficient and intelligent screening and real-time early warning of port concerned infectious diseases are realized.

The invention discloses preparation and application of grouper enterospora sporowall protein SWP26 and a polyclonal antibody of the grouper enterospora sporowall protein SWP26, and belongs to the field of animal quarantine, the grouper enterospora sporowall protein SWP26 is obtained by amplifying grouper enterospora genes by using a PCR (Polymerase Chain Reaction) method, shearing a target band and transferring the target band into a pET-32a vector to construct a recombinant plasmid, and then transforming escherichia coli BL21 (DE3) for induced expression. A Ni-NTA affinity chromatography method is used for protein purification, animal immunization and polyclonal antibody purification are carried out on the purified SWP26 recombinant protein, western blot detection is carried out on the purified antibody, an obvious signal appears at about 43kDa, and it is proved that the anti-SWP26 polyclonal antibody can be subjected to a specific reaction with the SWP26 purified protein. The invention clones and identifies the high-abundance spore wall protein SWP26 positioned on the surface of the enterosporidium of the grouper for the first time, belongs to a specific protein of the enterosporidium of the grouper, and can be used as a drug target for treating enterocytozoonosis of the grouper.

The utility model discloses a livestock quarantine excrement sampling device and belongs to the field of excrement sampling. The animal husbandry quarantine excrement sampling device comprises a fixed pipe and further comprises a plurality of flexible rods which are fixedly connected to the lower end of the fixed pipe at equal intervals in a circumferential mode; the fixed rod is arranged on the lower side of the flexible rod; the disposable viscose cloth is arranged on the lower sides of the fixing rods; the utility model effectively solves the problem that the dilute excrement is inconvenient to sample at present, effectively improves the sampling efficiency of the dilute excrement, and simultaneously effectively prevents the cotton swab from being in contact with the ground to be stained with excrement of other pigs when the cotton swab is used for sampling.

The invention discloses a loop-mediated isothermal amplification detection primer group for Rhodospora pini Rhodospora acuminata and an application of the loop-mediated isothermal amplification detection primer group for Rhodospora pini Rhodospora acuminata. The invention designs four groups of loop-mediated isothermal amplification detection primer groups of Rhodospora pini Maxillaris, which are respectively a primer combination T1, a primer combination R1, a primer combination R3 or a primer combination R5, of Rhodospora pini Maxillaris. Experimental results show that the loop-mediated isothermal amplification detection primer group and the loop-mediated isothermal amplification detection method of the pine needle red spot pathogen botrytis aculeatus by using the loop-mediated isothermal amplification detection primer group have the characteristics of high specificity, low requirements on instruments and equipment, convenience in operation and the like; in production practice, rapid, sensitive, accurate, safe and visual detection of the pine needle red spot pathogen can be achieved, the method can be used for field investigation and early diagnosis of the pine needle red spot pathogen, quarantine and transportation of nursery stocks and disease monitoring and identification, and reliable technical and theoretical bases are provided for early warning and prevention and control of the pine needle red spot pathogen.

The present invention relates to a movable port quarantine cabin, the purpose of which is to provide a flexible, well-insulated, effectively partitioned workplace for customs port quarantine operations to prevent cross-contamination. It is equipped with a trailer chassis and a body, which can move in the form of a vehicle and also provide a quarantine office space in the form of a house. The body is provided with side walls and a top cover, and the side walls are provided with doors. Inside the body, functional rooms such as a workroom, a sampling room, a disinfection room, a dressing room, etc. and an equipment room are effectively partitioned by wall panels. Disinfection devices and air change devices are provided in the functional rooms, and the layout of the functional rooms can prevent cross-contamination. The present invention can be widely applied to occasions where outdoor quarantine operations are carried out at customs ports.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

The application discloses a reagent, a method and application of the reagent and the method for simultaneously detecting three common viruses in a fancy carp, and belongs to the technical field of fish quarantine. The application provides a reaction system and a detection method for simultaneously detecting three common viruses in a fancy carp, establishes a multiplex fluorescence PCR method, adopts a full-closed reaction, solves the problem that common PCR is prone to generating aerosols and causing false positive results of tests, has high detection sensitivity, can detect target genes with a copy number of 10 orders of magnitude at the minimum, is suitable for detecting a small amount of viruses carried in normal fancy carp or viruses in water quality environment, and can fully meet the requirements of customs quarantine prevention and control, has strong specificity, has no cross reaction to templates such as IHNV, RSIV and EHNV, and further provides a virus-like particle which can be used as a positive reference product in the multiplex system, is stable in state and beneficial to storage, can monitor an extraction process, and ensures that a test is established.

The application discloses a waste stratified sealing and recycling device for health quarantine, which comprises a rail frame vertically fixed on a base plate, a discharging device arranged on the side of the rail frame, the discharging device being capable of reciprocating lifting along the extension direction of the rail frame, a treatment bin fixed on the upper part of the other side of the rail frame, a positioning and sealing assembly arranged at the lower end of the treatment bin, the positioning and sealing assembly being capable of sealing, packing and collecting the treated quarantine waste, and the discharging device being capable of fixing a garbage can and pouring the quarantine waste in the garbage can into the treatment bin.

The utility model discloses an inspection and quarantine box which comprises a box body used for storing quarantine equipment and provided with a closed structure to provide protection; the adjustable partition plate is arranged in the box body in a sliding mode so as to be matched with the storage equipment; the number of the detachable air bags is multiple, and the detachable air bags are detachably installed on the adjustable partition plate and the inner wall of the box body; wherein each detachable air bag is provided with a triggering type micro air pump, and after the triggering type micro air pumps are triggered, the detachable air bags are expanded to reach the pressure value so as to store equipment.

The invention belongs to the field of biological quarantine, and particularly relates to a method for separating and identifying pigeon-derived liver escherichia coli. Escherichia coli separation and identification are carried out on disease samples from Guangdong, Guangxi, Guizhou, Hainan and the like, and the epidemicity of the disease samples is determined; collecting livers of acute dead young meat pigeons, and separating strains; carrying out gram staining, physiological and biochemical identification and molecular identification on the separated strain; the growth and reproduction cycle and hemolysis characteristics of the strain are detected, 76 Escherichia coli infection occurs in 113 disease samples, and the infection rate is 67%. According to the method, laboratory diagnosis of infectious diseases of the meat pigeons is systematically completed through sample collection and separation, culture and identification of strains, it is determined that the young meat pigeons are infected by escherichia coli, and a foundation is laid for subsequent antibiotic-free green breeding of the meat pigeons resisting escherichia coli infection.

This utility model discloses a port risk management entry-exit quarantine device, including a storage box with several evenly distributed rollers at the bottom. A mounting plate is located on one side of the storage box, connected to the storage box via a connecting frame. Symmetrically arranged movable plates are located at the top of the storage box, and two sets of movable plates are connected to the mounting plate via a movable component. Beneficial effects: By setting the movable component, the two sets of movable plates can be adjusted to move synchronously relative to each other, allowing animal limbs to be quickly placed between clamping plates one and two. The movable component two can move clamping plates two closer to clamping plates one until the animal limbs are clamped tightly. The movable component two uses a screw rod two to engage with a movable connecting column for secure clamping. Both movable components one and two are driven by motors, eliminating the need for manual operation and saving time and effort.

The invention discloses a universal one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection primer and a kit for goose astrovirus, and belongs to the field of biotechnology and diagnostic detection. The primer comprises an upstream primer GAstV-F and a downstream primer GAstV-R, the sequences of the upstream primer GAstV-F and the downstream primer GAstV-R are respectively shown as GAstV-F / R. The kit comprises the primer. According to the GAstV-1 and GAstV-2 gene conserved regions, universal detection primers are designed, each reaction condition of an RT-PCR system is optimized, a universal one-step RT-PCR detection method for detecting GAstV is established, and the universal one-step RT-PCR detection method has the characteristics of specificity, sensitivity, rapidness, accuracy and the like, and can be used for laboratory diagnosis, customs quarantine and molecular epidemiological research of GAstV infection.

The utility model belongs to the technical field of quarantine and epidemic prevention equipment, and particularly relates to a livestock veterinary quarantine and epidemic prevention device which comprises a quarantine platform, the right end of the upper surface of the quarantine platform is fixedly connected with the bottom face of a second breast board, and the upper end of the left side face of the second breast board is fixedly connected with the right side face of a fixing frame. According to the utility model, the translation plate and the spray pipe are arranged above the quarantine platform, so that the reciprocating lead screw, the reciprocating lead screw nut and the translation plate can be used for driving the spray pipe to reciprocate left and right after quarantine is completed if the animal excretes incontinence on the quarantine platform in the quarantine and epidemic prevention process of the animal; according to the cleaning device for the animal quarantine, dirt on the grating plate is flushed through cleaning liquid sprayed out of the spraying pipe, the dirt on the grating plate is rapidly cleaned, then epidemic disease transmission caused by germs contained in excrement during follow-up quarantine of other animals is avoided, and the workload of veterinarians is also reduced.

The utility model relates to the technical field of livestock quarantine, in particular to a portable vaccine refrigerating box for livestock quarantine, which comprises a box body, a cover plate, a clamping ring, a first clamping block, a sleeve and the like. A cover plate is hinged to the top of the box body, a clamping ring is hinged to one side of the cover plate, a first clamping block is installed on the box body, a sleeve is installed on the box body, a positioning groove is formed in the sleeve, and a telescopic handle is installed on the sleeve in a sliding mode. The telescopic handle is pulled forwards, the second clamping block moves inwards after being extruded by the inner wall of the sleeve, the first elastic piece shrinks, the telescopic handle drives the second clamping block to move forwards, and when the second clamping block moves to the front end of the inner side of the sleeve, the first elastic piece resets, the first elastic piece drives the second clamping block to move inwards, and the second clamping block moves forwards. And the second clamping blocks are embedded into the positioning grooves, so that a veterinarian can easily pull the box body to move, and the fatigue degree of the veterinarian when the veterinarian carries the vaccine refrigerating box on the shoulder is reduced.

This invention discloses a livestock and veterinary quarantine and testing device, relating to the field of quarantine and testing technology. The device includes a placement base, a fixing frame mounted on top of the base, and a power rod driven by an electric push rod mounted on the bottom of the fixing frame. A blood sampling tube body is mounted on the bottom of the power rod. When an animal is placed on a placement plate within the base, the electric telescopic rod is activated. This, along with a moving rod, an arc-shaped outer plate, an elastic telescopic rod, an arc-shaped inner plate, a hydraulic chamber, a force-bearing rod, a push plate, a spring, a spring, and a pressure plate, allows for initial fixation of the animal before blood sampling. Further fixation is performed during blood sampling, and the device quickly returns to the initial fixation state after sampling, reducing clamping time and minimizing the possibility of injury to the animal.

The invention provides a pine wood nematode intelligent detection method based on deep learning. The pine wood nematode intelligent detection method comprises the following steps: acquiring an original microscopic image set by using a microscopic imaging hardware system; performing basic preprocessing, key part identification and labeling and data enhancement operation on the original microscopic image set to obtain a high-quality training sample set; an improved YOLOv11 detection model is constructed; using a YOLOv11 detection model to output candidate boxes and a prediction probability, and screening and positioning the candidate boxes to obtain a double-ROI image and coordinate information; respectively extracting a tail parameter set and a closet parameter set to obtain a unified composite feature vector; and then carrying out online deployment on the YOLOv11 detection model and intuitively displaying a detection result. According to the method, key organs such as vulva caps, joint thorns and tail shapes of the pine wood nematodes can be automatically positioned and recognized under the microscopic imaging condition, and rapid judgment and auxiliary quarantine of pine wood nematode pathogens are achieved.

1. Name of the product in this design: Slaughterhouse Quarantine Hand Grip Hook. 2. Purpose of this design: for use in slaughterhouses for hooking and hanging livestock. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D view 1.

The present invention relates to a disaster area disinfection drone, wherein the disaster area disinfection drone is controlled by a server, and the drone device is mounted on a drone mount to charge disinfectant solution and power, and unfolds the flight unit to prepare for flight, and the drone device detects the drone's location and surroundings through a sensor, flies to the disaster area, reaches the disaster area and sprays disinfectant solution, and returns to the drone mount when the disinfectant solution or power is nearly consumed or when disinfection is completed, and the drone device comprises a power unit for charging power, a flight unit for flying, a sensor unit for detecting surroundings at the top, and a disinfection unit at the bottom for storing and spraying disinfectant solution, and the drone mount is controlled by a server and may include a charging unit for charging disinfectant solution and power when the drone device is mounted on the drone mount.

The utility model relates to the technical field of container fumigation quarantine, and discloses a port import container fumigation quarantine device which comprises a quarantine device body, a connecting block is fixedly connected to the outer wall of the quarantine device body, and a fixing plate is fixedly connected to the side, away from the quarantine device body, of the outer wall of the connecting block. A clamping assembly is arranged in the fixing plate, a sulfuryl fluoride tank is slidably connected into the fixing plate, the clamping assembly comprises a telescopic rod, one end of the telescopic rod is fixedly connected to the inner wall of the fixing plate, the other end of the telescopic rod is fixedly connected with a first extrusion block, and the outer wall of the telescopic rod is slidably connected with a first spring. According to the fumigant storage tank fixing device, a worker pushes the fumigant storage tank by hand to extrude the first extrusion block to enable the first extrusion block to enter the groove of the fixing plate, and the first extrusion block loses stress and returns to the original position to block the mounting opening, so that the time required for replacing the fumigant storage tank is shortened, and the working efficiency is improved.

The application discloses a livestock breeding poultry quarantine sampling device, and particularly relates to the technical field of poultry quarantine sampling, and comprises a positioning disc, a positioning sampling assembly is arranged on the positioning disc, the positioning sampling assembly comprises a swivel ring arranged on one side of the positioning disc, a screw rod capable of being displaced up and down is slidly connected to the middle part of the swivel ring, and a top disc is movably connected to the top end of the screw rod. The application effectively removes interfering substances in the sample, avoids the influence of impurities on subsequent pathogen detection results, improves the reliability of quarantine data, and improves the accuracy and comprehensiveness of quarantine results. The blood sample is used for detecting pathogen antibodies, immune state and other indexes, and the fecal sample is used for checking intestinal pathogens, parasite eggs and the like. The two types of samples are complementary and verified, the accuracy and comprehensiveness of the quarantine results are greatly improved, and the problem that a single sample cannot comprehensively judge the health status of poultry is effectively solved.

The invention discloses a primer probe composition, a kit and a method for detecting various cattle and sheep fever and respiratory tract pathogens based on nucleic acid mass spectrometry, and relates to the technical field of microbiological detection, a multiple PCR primer composition and a single-base extension probe group are innovatively designed, conservative stable regions in various pathogen genomes can be targeted, and the detection sensitivity is high. Single-tube multi-target synchronous amplification is realized, and further extension amplification is carried out, so that an extension product suitable for MALDI-TOF MS detection is obtained. The method realizes multi-target, high-sensitivity and automatic synchronous detection of fever pathogens of cattle and sheep, provides a basis for rapid detection of nucleic acids of multiple pathogens, and has a good application prospect in quarantine and prevention and control.

The invention belongs to the technical field of parasite sampling, particularly relates to an imported marine fish pathogenic parasite sampling and detecting device, and provides the following scheme aiming at the problems of low manual sampling efficiency, inaccurate positioning and easy sample damage in existing marine fish parasite quarantine. Comprising a sampling base, a movable supporting plate, a sampling area identification assembly, a sampling pipe and a negative pressure separation assembly. The sampling area identification assembly can losslessly and accurately position a suspected parasite infection area in a fish body. The sampling pipe is provided with an annular gas guide cavity and an annular liquid guide cavity, sterile gas and normal saline can be sprayed cooperatively, and minimally invasive and efficient stripping and recycling of insect bodies are achieved. The negative pressure separation assembly adopts a double-chamber design, a micro-filtration membrane is arranged in the negative pressure separation assembly, a sample can be shunted and independently treated in single operation, and a negative pressure liquid extraction pipe of the negative pressure separation assembly has an automatic valve function of negative pressure ventilation and normal pressure closing, so that subsequent in-situ fixation is facilitated. According to the invention, the whole process automation from precise minimally invasive sampling to automatic separation and fixation is realized.

The utility model discloses veterinary quarantine parasite sampling and collecting equipment, which belongs to the technical field of parasite sampling and collecting and comprises a collecting box, a fixed box is fixedly connected in the collecting box, a first shell is fixedly connected to the side surface of the fixed box, a first driving motor is fixedly mounted in the first shell, and a second driving motor is fixedly mounted in the first shell. First bearings are fixedly connected to the two sides of the inner wall of the fixed box, first rotating shafts are rotationally connected into the two first bearings, one end of one first rotating shaft is fixedly connected with an output shaft of a first driving motor, and the first driving motor works to drive a lead screw to rotate; a second driving motor works to drive a second rotating shaft to rotate, the purpose that fan blades quickly convey mist into a collecting box is achieved by means of a small atomizer and a through hole, and the effect of humidifying the sample is achieved.