376 results about "Epidemiology" patented technology

The present invention provides methods for rapid forensic investigations by identification of bioagents associated with biowarfare and acts of terrorism or crime. The methods are also useful for epidemiological investigations by genotyping of bioagents.

Materials and methods for identifying unique sites in bacterial 16S and 23S rDNA are provided, as well as specific unique sequences of 16S rDNA in select bacteria. The distinguishing moieties will enable rapid differentiation between families, genera, groups, species, strains, subspecies, and isolates of microorganisms. Such differentiation can be performed by using rapid screening kits in combination with in silico analysis for diagnostic, prognastic, epidemiologic, phylogenetic, and other purposes.

The system has number of environmental sensors to sense various environmental parameters of the personal environment envelope surrounding the individual and multiple biological sensors to sense biological and physiological condition of the individual. The system includes a personal environment envelop setting module to define preferred settings based on input from the individual. The system has a processing module to process the environmental parameters sensed by the sensors and the preferred settings to generate alerts. The processing module, also receives information from other sources and is capable of dynamically compute recommendations, map these with the preferences of the individual for enabling the preferred conditions. The system also has a server module configured to receive, store, analyze and machine learn from data of the plurality of environmental parameters sensed by the plurality of sensors and the preferred settings along with ambient environmental data from plurality of persons for epidemiology study.

A proximity network map defines who and what objects have come in contact of each other including location and time. This map selects the list people who have come in contact with known infected people based on contagious disease epidemiology criteria to control its spread, or to prevent radiation poisoning, limit bio-chemicals exposure, etc. These people then undergo testing and quarantine procedures. It monitors hygiene practices and reduces nosocomial infections in hospitals and mitigates the pandemic flu threat by controlling contamination. It controls people interaction, information flow in a high security environment, control crime or gang activities. Each person or object carrying a proximity-sensing unit with unique ID records all units it encountered over a period of time. This information is stored with time stamp in a relational database and transferred to network servers. The databases are then replication throughout via a central web database server for retrieval and analysis.

A computer program establishes a first interface with a hospital cardiology imaging system and a second interface with a hospital radiology imaging system. The program generates a user interface that enables a user to interact with the cardiology imaging system (20c) and the radiology imaging system (20b) via the computer system. The user interface also presents patient information relating to medical history (302), demographics (308), epidemiology (312), genetics (314), and studies (310) the patient is involved in.

A robust, automated computational pipeline was used to design a system comprising a microarray for the identification of microorganisms and their antibiotic resistance profiles. This system and methods will facilitate the study of the epidemiology and microbial ecology of antibiotic resistance and be an invaluable tool to rapidly and simultaneously identify organisms and their antimicrobial resistance elements in environmental, food and clinical samples.

Described herein are novel SCCmec right extremity junction (MREJ) sequences for the detection and / or identification of methicillin-resistant Staphylococcus aureus (MRSA). Disclosed are methods and compositions based on DNA sequences for the specific detection of MREJ sequences designated types xi, xii, xiii, xiv, xv, xvi, xvii, xviii, xix, and xx for diagnostic purposes and / or epidemiological typing.

Methods for typing a strain of an organism are provided, the methods comprising the steps of amplifying, in a single reaction mixture containing nucleic acid from the organism, dividing the reaction mixture into a plurality of sets of second-stage reaction wells, each set of second-stage reaction wells containing a different pair of second-stage primers, subjecting each of the second-stage reaction wells to amplification conditions to generate a plurality of second-stage amplicons, melting the second-stage amplicons to generate a melting curve for each second-stage amplicon, and identifying the strain of the organism from the melting curves.

The invention relates to a city-wide infectious disease simulation method and a city-wide infectious disease simulation device. The method includes the steps of: 1) partitioning the city and classifying the people; 2) building a 4p ordinary simultaneous differential equation set which describes the dynamic process of the transmission of the disease in p (number) areas; 3) discretizing the equation set to obtain a difference equation set; 4) converting the discretized difference equation set into an open equation set by taking the connections of other cities into consideration; and 5) resolving the parameters of the equation set and visually expressing the infected people in the manner of dot density and piled bar graph. According to the epidemiological characteristics of the infectious disease and based on the data of interregional population flow across the city, the city-wide infectious disease simulation method and the city-wide infectious disease simulation device can predict and simulate the dynamic contagion process of the disease spatially in a period of time in the future, and the spatial propagation process of a typical infectious disease can be analyzed on a map in a visual way. The city-wide infectious disease simulation method and the city-wide infectious disease simulation device provide a decision support to infectious disease propagation prevention and control.

The invention discloses a vaccine strain H5N1 / PR8-5B19 marked by H5N1 hypotype poultry influenza virus, which also provides the application to prevent and monitor poultry influenza.

A real-time assay coupled with a non-invasive tissue sampling was developed for the detection and quantification of fish viruses. As a proof of principles, data were presented for the detection and quantification of infectious hypodermal necrosis virus (IHNV) in trout. The primers were designed for IHNV nucleocapsid (N), and surface glycoprotein (G) genes, and trout &bgr;-actin and elongation factor-l&agr; (EF-I &agr;) were used as internal control for the assay. The reaction conditions for the real-time RT-PCR were optimized using cDNA derived from IHNV-infected Epithelioma papulosum cyprinid (EPC) cells. Using both N- and G-gene primers, IHNV was successfully detected in liver, kidney, spleen, adipose tissue and pectoral fin samples of laboratory-challenged and wild samples. The dissociation curves with a single melting peak at expected temperature (85° C. for the N-gene and 86.5° C. for the G-gene) confirmed the specificity of the N- and G-gene amplicons. The IHNV N- and the G-gene expression levels in different tissues of laboratory challenged samples were in the order of spleen, liver, kidney, adipose tissue and pectoral fin, however in the field-collected samples the order of gene expression was liver, kidney, pectoral fin, adipose tissue, and spleen. The N- and G-gene expressions in spleen were found to be dramatically lower in the field-collected samples compared to the laboratory-challenged samples indicating a potential difference in the IHNV replication in the laboratory as opposed to field conditions. The real-time PCR assay was found to be rapid, highly sensitive, and reproducible. Based upon the ability to detect the virus in pectoral fins a non-invasive detection method for IHNV and other fish viruses is developed. Such a non-invasive tissue sampling coupled with real-time PCR assay is very valuable for large-scale virus screening of fish in aquaculture facilities as well as for epidemiological studies.

The invention aims at providing an I-colony fowl adenovirus 4 strain, which is preserved with preservation number of CCTCC No. V201541. The I-colony fowl adenovirus 4 YBAV-4 strain disclosed by the invention is excellent in specificity and immunogenicity; a specific precipitation line does not appear in specific positive serum chicken SPF chicken serum such as infected cell sap and egg drop syndrome resisting virus, chicken infectious bursal disease virus, Newcastle disease virus, chicken infectious laryngotracheitis virus, chicken Marek's disease virus, avian influenza and the like, while an obvious specific precipitation line appears in I-colony fowl adenovirus 4 specific serum. The strain disclosed by the invention, as a vaccine strain which is good in manufacturing effect, is capable of preventing chicken hydropericardium syndrome, and the strain is applicable to identification of virus serotype and investigation on epidemiology.

The invention provides a fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) kit for detecting an influenza A virus subtype H7N9. The fluorescent quantitative RT-PCR kit can be used for detection of influenza A viruses and the influenza A virus subtype H7N9. The fluorescent quantitative RT-PCR kit comprises a quantitative RT-PCR reaction solution, an enzyme mixed liquor, a primer and probe mixed liquor, standard substances of influenza A viruses, H7, N9 and RNaseP, positive reference substances of influenza A viruses, H7, N9 and RNaseP), and negative reference substances. Specific primers and probes are designed according to conserved sequences of influenza A viruses, H7 and N9. The RNaseP primers and probes are used as internal references. Through the one-step quadruple real-time fluorescent RT-PCR technology, the influenza A virus and the influenza A virus subtype H7N9 in the sample can be fast and accurately detected. The fluorescent quantitative RT-PCR kit has a reasonable design, very high singularity, sensitivity and repeatability, can be used for laboratory emergency diagnosis and fast screening of an epidemic disease caused by the influenza A virus subtype H7N9, and for an epidemiology study on the influenza A virus and the influenza A virus subtype H7N9 causing fever and respiratory tract syndrome.

The invention discloses a kit for detecting respiratory viruses and an application thereof. The kit comprises a primer pair group for detecting the respiratory viruses, wherein the primer pair group consists of 16 primer pairs, the sequences of which are shown in sequences 1 to 32 in a sequence table. The kit provided by the invention supports high throughput, can be used for rapidly and accurately detecting the infection of conventional respiratory viruses, and can clinically acquire a detection result of 16 virus indexes in one hour, which is not only faster than the real-time fluorescence quantitative PCR method generally used at present, but also has an important significance for rapidly assisting and instructing the treatment and medicine application. Meanwhile, the multi-index detection can also be used for investigating regional epidemiology and monitoring the epidemic situation so as to research the prevalence state of respiratory virus infection in China.

The invention discloses an on-site epidemiology intelligent investigation system. The system is composed of a handheld mobile terminal and a server, wherein the handheld mobile terminal and the server are interconnected via a network; the handheld mobile terminal is a mobile working application carrier, comprises a one-dimensional and two-dimensional code scanning and communication interface and a data line interface, and further comprises the following functional modules: a data display module, a data acquisition module, a graphic image acquisition module, a data local storage module and a wireless communication functional module; and the server comprises an application server, a data server and a file server, and further comprises the following functional modules: an epidemic situation management module, a knowledge base management module, a disease monitoring management module, a system management module and a forum management module. Users can choose to use a PC (Personal Computer) terminal mode or a handheld mobile terminal mode for data acquisition and query works according to site conditions, the data is transmitted in a wire transmission form or wireless transmission form, the use flexibility and compatibility of the system can be ensured, and the system exerts the effects of practical investigation works to the greatest extent.

The invention discloses a multiple PCR method for simultaneously detecting and identifying three human tissue echinococcosis pathogens, including echinococcus granulosus G1-G3, echinococcus multilocularis and echinococcus granulosus G6-G10. According to the invention, 3 pairs of specific primers are designed for the mitochondrial genes of 3 pathogens; the specific primers are added into a same PCR reaction system; the specific primers are complementarily combined with the target genes of the corresponding parasite species; through amplified reaction, different parasite species can generate target stripes in different sizes; gel electrophoresis is adopted for separating and detecting. The three pairs of primers according to the invention are free from mutual interference and have no cross reaction with 8 tapeworms in close genetic relationship or do not interfere with the result judgment. The method is high in specificity and sensitivity, can effectively and accurately realize the synchronous detection for 3 pathogens, can greatly save the detection time and cost, can effectively increase the working efficiency and can be applied to the research on the parting identification for human echinococcosis pathogens and echinococcosis molecular epidemiology.

The invention provides a quick test dipstick used for testing a specific antigen of Brucella, which comprises a reaction membrane and a binder release liner. The reaction membrane is provided with a testing strap coated with a bp26 monoclonal antibody or bp26 polyclonal antibody and a quality control strap coated with anti-IgG; the binder release liner is coated with the bp26 monoclonal antibody which is marked by colloidal gold to be different from an antigen binding site on the reaction membrane. A membrane chromatography double antibody sandwich method is applied for detecting the specific antigen of the Brucella in a specimen. As the dipstick is adopted for test, the operation is simple, convenient, quick and concise, requiring no special equipment and facilities as well as professional training. Furthermore, the dipstick has clear and easy-identity results, simple operation and easy popularization, is applicable to matrixes, field tests of emergency on a large scale and the study of epidemiology and can aid in the infection diagnostics of the Brucella.

The invention belongs to the biotechnology application field, and relates to simultaneous detection and genotyping of infections of 16 respiratory viruses (including FluA, FluB, sH1N1, PIV1, PIV2, PIV3, RSVA, RSVB, HRV, HMPV, HBoV, CoV NL63, CoV OC43, CoV 229E, CoV HKU1 and Adv) of nasopharyngeal extract specimens of patients of respiratory-related diseases of all levels of disease prevention and control institutions and sentinel hospitals. Specifically, nucleotide sequences of representative strains of the 16 respiratory viruses are downloaded from NCBI; through literature review and multiple sequence alignment, pathogen relatively-conservative regions are determined, and multiplex specific primers are designed. Single-tube multiplex (18 stages) PCR detection is carried out for detecting the 16 respiratory virus conservative regions, and an entire reaction takes less than 2 hours. According to the invention, a defect that genotyping cannot be carried out with conventional single-tube multiplex fluorescent qualitative PCR detections can be overcome, and defects of complicated operations, long time, and high cost of conventional chip detection methods can be overcome. With the application provided by the invention, a novel idea is provided for respiratory virus genotyping technologies. With characteristics of high specificity, high sensitivity, and high speed, powerful technological support is provided for rapid and accurate screening and genotyping of the respiratory-disease-related viruses. The invention has important significance upon the researches of respiratory-tract patient infection pathogen spectrum of out nation, and upon molecular epidemiological investigations.

The invention provides an intelligent epidemic prevention gate machine system, and the system is used for carrying out non-inductive body temperature detection on people passing through a ticket checking channel one by one; the system is accurate in detection and high in efficiency, and enables a subsequent database to carry out epidemiological development prediction, tracking and analysis according to actual conditions. The system comprises a gate array, and the gate array comprises a plurality of ticket checking channels. A thermal imaging sensor is fixedly arranged on the upper shell of theticket checking gate corresponding to each ticket checking channel; the thermal imaging sensor is arranged within one meter towards the face of a person to be checked, and the bottom of the thermal imaging sensor is connected to a ticket checking module in the ticket checking gate through a data line; each ticket checking module is further connected to an external data switch through a data line,and the data switches are externally connected to a host system through data lines; the host system records and analyzes the obtained body temperature data of passing persons in real time, and the output ends of the ticket checking modules are externally connected to the corresponding baffle sets respectively.

(3R,3′R,6′R)-Lutein and (3R,3′R)-zeaxanthin are two dietary carotenoids that are present in most fruits and vegetables commonly consumed in the US. These carotenoids accumulate in the human plasma, major organs, and ocular tissues. In the past decade, numerous epidemiological and experimental studies have shown that lutein and zeaxanthin play an important role in the prevention of age-related macular degeneration (AMD) that is the leading cause of blindness in the U.S. and Western World. The invention provides a process for the synthesis of (3R,3′R,6′R)-lutein and its stereoisomers from commercially available (rac)-α-ionone by a C15+C10+C15 coupling strategy. In addition, the present invention also provides access to the precursors of optically active carotenoids with 3-hydroxy-ε-end group that are otherwise difficult to synthesize. The process developed for the synthesis of lutein and its stereoisomers is straightforward and has potential for commercialization.

The invention discloses an efficient epidemic prevention system and method implemented by using wearable equipment. Identity identification and binding are realized through the FRID. A receiving end is arranged at the entrance and exit of the field; manual temperature measurement and code scanning are not needed at an entrance and an exit, receiving ends are arranged in different sub-places respectively, positioning management and temperature measurement second-level implementation of the user are achieved, manual intervention is not needed, and when the user with abnormal body temperature passes through the entrance receiving end, the background automatically displays the name and body temperature of the abnormal user, prompts security intervention and pays attention to personal protection; once a definite case occurs, if the patient is in direct close contact with the patient, the system background calls related data, the contact time and the contact place of the direct close contactperson and the indirect contact person of the patient can be quickly and accurately positioned, epidemiological investigation is completed, accurate isolation and medical observation are implementedby adopting broken and appropriate measures, and normal production and teaching are not affected.

The invention relates to three reagent kits for detecting human parainfluenza viruses 1, 2, 3 type real-time fluorescence polymerase chain reaction, which respectively adopts a one-step method RT-PCR, a two-step method RT-PCR and a multicolor fluorescence method RT-PCR to distinguish types of the human parainfluenza viruses of multi-type specimens and fix an amount of the human parainfluenza viruses of the multi-type specimens. The detection methods of the reagent kits have simple operation, short consuming time and high sensitivity and specificity and can be extensively used in a plurality of fields, such as the auxiliary diagnosis of the infection of the human parainfluenza viruses, clinical medicine direction, epidemiology retrospective study, and the like.

The invention belongs to the biotechnological application filed, and relates to simultaneous detection and typing of the infection of nine encephalitis viruses (comprising banna viruses, GI encephalitis B viruses, GIII encephalitis B viruses, tick-borne encephalitis viruses, Tahyna viruses, Liaoning viruses, Kyasanur forest fever viruses, Sindbis viruses and Yunnan orbiviruses) of viral encephalitis patient specimens in disease prevention control mechanisms at all levels, sentinel point hospitals and the like. The nucleotide sequences of nine encephalitis related virus strains are downloaded from NCBI, the pathogen relative conservation region is determined through literature consulting and multiple sequence alignment, and multiple specific primers are designed. The single tube 13-plex multiplex PCR test is carried out to detect nine encephalitis virus conservation regions, and the consumption time of the whole reaction is less than 2h. According to the invention, the non-typing disadvantage of routine single-tube multiple fluorescent qualitative PCR detection is overcome, the disadvantages comprising complex operation, long time, high cost and the like of routine chip detection methods are overcome, a new thought is provided for an encephalitis virus typing technology, the characteristics comprising high specificity, high sensitivity and rapidness of the GeXP multiple gene expression heredity analysis system provide a strong technological support for the rapid and accurate screening and typing of the encephalitis viruses, and the GeXP multiple gene expression heredity analysis system is of important significance to the encephalitis syndrome patient infection pathogen spectrum and molecular epidemiology investigation in China.

The invention discloses a multiple PCR detection kit for virulence factors of streptococcus suis and a detection method thereof, and belongs to the technical field of biology. The multiple PCR detection kit comprises nucleic acid shown by base sequences, such as SEQ ID NO:1 to SEQ ID NO:20. The detection method for the multiple PCR detection kit comprises the following steps of: 1, providing DNA of samples to be detected; 2, taking the kit, amplifying the DNA of the samples to be detected by adopting the conventional PCR method, detecting an amplified result by adopting an agarose gel electrophoresis method, and judging according to the result; and by using positive DNA of streptococcus suis 2 type as contrast, if the contrast proves that not all the strips are amplified, re-detecting, and if the contrast proves that all the strips are amplified, judging the result. The multiple PCR detection method established by the invention is specific and sensitive, and has the advantages of simpleness, convenience and quickness. The multiple PCR detection kit has reliable stability, and can be used for rapid detection of clinical samples and survey research on molecular epidemiology for the streptococcus suis.

The invention belongs to the technical field of molecular biology, and particularly relates to a real-time fluorescent quantitative PCR primer, kit and detection method for detecting the porcine circovirus 3. The nucleotide sequence of the real-time fluorescent quantitative PCR primer for detecting the porcine circovirus 3 is shown in SEQ ID NO.1-2. The invention further provides the kit containing the primer mentioned above and the detection method for detecting the porcine circovirus 3. The primer, the kit and the detection method are high in specificity, high in sensitivity and stable in repeatability, wave crests of the melting curve of amplified products are single, no primer dimer is caused, and the primer and the kit have no cross reaction with genomes of the porcine circovirus 2, the hog cholera virus, the porcine pseudorabies virus and the porcine reproductive and respiratory syndrome virus; sensitivity is 102 copies per microliter and is 100 times higher than that of the conventional PCR; and the variation coefficients of inter-group and intra-group repeated tests are all smaller than 3.0%. The foundation is laid for research on molecular epidemiology, prevention and control of the pestilence.

The invention discloses a multiple RT-PCR quick detection primer group for five porcine enteroviruses. The primer group comprises five pairs of PCR primers which respectively aim at the five porcine enteroviruses PEDV, TGEV, PDCoV, PRoV and PKV and respectively have base sequences shown in a sequence table from SEQ. ID. NO.1 to SEQ. ID. NO.10. According to this, the inventor further develops a corresponding quick detection kit. The primer group has the advantages of high specificity, high sensitivity, short time consumption and low cost when being applied in a same reaction tube to detect single or mixed infection of the five porcine enteroviruses at the same time. A quick, simple and convenient tool is provided for detection of swinery mixed infection, a solid foundation is laid for clinical quick identification and detection and lab epidemiological investigation, timely formulating of treatment plans in porcine industry is facilitated, and swinery death rate and economic loss are reduced.