The invention discloses a kit of dog echinococcus granulosus and echinococcus multilocularis based on POCKIT Micro fluorescent PCR platform, and application of the kit. The kit comprises fluorescent quantitative PCR reaction fluid, positive comparison product and negative comparison product; the fluorescent quantitative PCR reaction fluid comprises upstream general primers of echinococcus granulosus and echinococcus multilocularis, downstream general primers of echinococcus granulosus and echinococcus multilocularis, general probes of echinococcus granulosus and echinococcus multilocularis, Taq enzyme; premixing buffer and deionized water. The nucleotide sequence of the upstream general primers of echinococcus granulosus and echinococcus multilocularis and the nucleotide sequence of the downstream general primers of echinococcus granulosus and echinococcus multilocularis are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The kit design is specific to the general primers and the probes of two hydatids; the result can be determined by detection once, thus the time is greatly saved, and detection cost is reduced.