2 results about "Egg drop syndrome" patented technology

The application relates to the technical field of traditional Chinese medicine preparation, and particularly discloses a compound traditional Chinese medicine preparation for targeting egg duck and goose egg cytoplasmic disease, which comprises the following components: white head, Coptis chinensis, Huangqi, Phellodendron amurense, wine Danpi, bran-fried peach kernel, Chinese perilla, pomegranate peel, liquorice, honeysuckle, Andrographis, figwort, emblic, ginger, fried atractylodes, fried safflower, Xianhecao, spiny amaranth, and Poria cocos. Through the combination of monarch, minister, assistant and messenger, the core effects of heat-clearing and detoxifying, blood-cooling and dysentery-stopping, and dampness-drying and insect-killing of the medicines are synergistically exerted, the pathogenesis characteristics of the egg cytoplasmic disease are accurately targeted, blood stasis and blood circulation are promoted to eliminate the swelling of the lesion, and the intestinal mucosa damage is repaired to stop bleeding, the symptoms of hematochezia, diarrhea and oviduct inflammation of the egg duck and goose caused by the egg cytoplasmic disease are effectively relieved, the problems of the decreased antibiotic effect, drug residue, bacterial flora disorder and food safety risk are effectively solved, and the compound traditional Chinese medicine preparation has the remarkable progress of drug resistance, safety, no residue, intestinal protection and green environmental protection.

The invention relates to a method for efficiently producing tFiber antigen protein of poultry egg drop syndrome and application of the tFiber antigen protein, and belongs to the field of animal biological products. The invention relates to a method for efficiently producing soluble poultry egg drop syndrome tFiber antigen protein. The method comprises the following steps: (1) inoculation: inoculating recombinant escherichia coli for expressing the poultry egg drop syndrome tFiber antigen protein into a basic culture medium of a large-volume fermentation tank; (2) an enrichment stage; (3) a chemical partner addition and target protein induction stage: when the OD600 value reaches 30, adding 5-20 mmol / L of chemical partner betaine, cooling to 32 DEG C after 2 hours, controlling DO to be 20-30% through the stirring speed, the ventilatory capacity and the feeding speed, stabilizing for 15-30 minutes, adding IPTG to induce target protein expression, and inducing by adopting a temperature control method related to yield; and (4) collection: after the induction stage is ended, centrifugally collecting bacterial sludge. According to the method disclosed by the invention, the soluble expression quantity of the tFiber protein is improved.