394 results about "Newcastle disease" patented technology

The invention relates to a composition for widely treating viral diseases. The composition contains the following raw materials, or extracts of the following raw materials, or derivatives of the extracts of the following raw materials in chemically-acceptable forms, wherein the raw materials include processed products prepared from the raw materials. The constituents of the composition are as follows: 100 parts of Baikal skullcap root, 50-150 parts of radix bupleuri and 50-150 parts of kudzu root; and according to specific circumstances, one or more selected from (but not limited to) the following raw materials can be added on this basis: radix isatidis, honeysuckle flower, Fructus Forsythiae, rhubarb, phellodendron bark, rhizoma anemarrhenae, Cyrtomium fortunei, gardenia, coptis, rhizoma cyperi, fritillaria, balloonflower and licorice root. The composition provided by the invention can be prepared into powder, liquid, paste, granules, capsules or other pharmaceutically-acceptable physical forms. The composition provided by the invention is used for preventing and treating avian influenza, epidemic hemorrhagic fever, epidemic parotitis, nameless hyperpyrexia, Newcastle diseases, swine fever, blue-ear diseases and other multiple human and animal viral diseases characterized by hemorrhage or body temperature rise, and reducing the degree of pathological damage to the body.

The present invention provides a novel avian herpesvirus (NAHV) vector and recombinant vaccines made therefrom that are useful to immunize avian species against Marek's disease, infectious laryngotracheitis and Newcastle disease. Methods of immunizing an avian species against Marek's disease, infectious laryngotracheitis and Newcastle disease are also provided.

The invention discloses a Chinese medicinal herb additive for meat chickens and application. The Chinese medicinal herb additive comprises heterophylly falsestarwort root, nutgrass galingale rhizome, amur corktree bark, coptis root, medicated leaven, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, cyrtomium fortune, chatoyancy and calamus; and mixture of the compositions is added with calcium bicarbonate, phytase, mulitivitamin for birds and lysine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can resist bacteria and virus, strengthen immunity of the meat chickens, effectively prevents bird flu, has obvious curative effect on diseases having respiratory tract symptoms as the major symptom, and has inhibiting function on familiar newcastle disease, colon bacillus, Pasteurella, enteritis, diarrhea, feather pecking, hair shedding, anus pecking, coccidiosis and the like. The additive cannot restrict the growth and disease resistance of the meat chickens, the meat quality of the meat chickens fed by the additive is obviously better than that of the meat chickens fed by the prior feed additive, the average daily gain of the meat chickens is improved by 23 to 28 percent than that of the meat chickens fed by the prior feed additive, the cultivation cycle is obviously shortened, and no harmful substance is deposited.

The invention provides a feed additive prepared from traditional Chinese medicines and used for preventing and treating common diseases of chickens. The feed additive is prepared from the following raw medicines by weight: honeysuckle, rhizoma belamcandae, rhizoma atractylodis, hawthorns, baical skullcap root, rhizoma coptidis, amur corktree bark, radix astragali, radix isatidis, rabdosia rubescens and dandelions. A preparation method of the feed additive is characterized by mixing the above raw medicines and then grinding the mixture or respectively grinding the above raw medicines and then mixing the ground raw medicines. The feed additive has the functions of strengthening body resistance and eliminating evil, clearing away heat and eliminating toxins, diminishing inflammation and strengthening stomach and killing bacteria and pests. By being mixed into the daily feed, the feed additive enhances the immunity of the chicken bodies, improves the disease resistance and has good prevention and treatment effects on common diseases of chickens such as bird flu, newcastle disease, salpingitis, white diarrhea, cholera, ascariasis, laryngotracheitis, coccidiosis, escherichia coli and the like. Through applications, the feed additive has the advantages of good prevention and treatment effects, no toxic or side effect, easily obtained raw materials and low price.

The invention discloses a genetic engineering marked attenuated vaccine strain of a porcine reproductive and respiratory syndrome virus (PRRSV). The attenuated vaccine strain comprises a genomic nucleic acid of a porcine reproductive and respiratory syndrome virus attenuated vaccine strain HuN4-F112; the HuN4-F112 genome includes a mutation in a genetic region for coding an Nsp2 protein, and the mutation is as follows: a nucleotide sequence for coding a Newcastle disease virus NP protein is inserted to a lacking region of a nucleotide sequence for coding 480-532-site amino acid of the Nsp2 protein; or the nucleotide sequence for coding the Newcastle disease virus NP protein is inserted to the lacking region of a nucleotide sequence for coding 508-532-site amino acid of the Nsp2 protein. The invention also discloses an application of the genetic engineering marked attenuated vaccine strain. The genetic engineering marked attenuated vaccine strain of the porcine reproductive and respiratory syndrome virus provided by the invention not only can provide completely safe immune protection to resist high-pathogenicity PRRSV after the porcine is immunized, but also can effectively distinguish the immunized porcine of the porcine reproductive and respiratory syndrome vaccine with the naturally infected porcine of the field virus.

The invention belongs to the immunity application field, which relates to the related fields of animal molecular biochemistry and the immunology, etc. The invention discloses a reagent kit used for fast testing the antibody of Newcastle Disease, which comprises a case body, and the test paper card and sample diluent equipped inside the case body, wherein, the test paper card is formed by the absorption pad, nitrocellulose membrane, gold-label pad and sample pad affixed in turn on the non-absorbent supporting flake by taking the sample pad, absorption pad and the HN recombinant proteins coated with the Newcastle Disease as the detection line and the HN recombinant proteins coated with anti-mouse IgG as nitrocellulose membrane of the quality control line. The reagent kit used for testing the corresponding antibody has the obvious advantages of strong specificity, high sensitivity, easy operation and fast diagnosis.

The invention provides a porcine pseudorabies virus vaccine composition. The porcine pseudorabies virus vaccine composition contains a porcine pseudorabies virus subunit antigen, or a recombinant Newcastle disease virus, namely a porcine pseudorabies virus vector. The invention further provides the preparation method and application of the vaccine composition. The vaccine composition can effectively prevent related diseases of a porcine pseudorabies virus and related diseases infected by the porcine pseudorabies virus. The combination of immunogenicity antigens in the porcine pseudorabies virus vaccine composition can be induced to generate a synergetic immune effect, thereby having good immune effect, and further lowering the immune usage amount to lower the immunization cost.

The invention provides a preparation method for a triple inactivated vaccine of recombinant Newcastle disease, bird flu and infectious bronchitis. The technical scheme is realized by gene modification of a Newcastle disease virus (DNV) LaSota strain. A recombinant NDV low virulent strain (rLaSota strain) is taken as a vector, firstly an S1 gene of a chicken infectious bronchitis virus (IBV) is inserted between F-HN, a recombinant NDV rLaSota/S1 expressing an S1 protein is constructed by utilizing a reverse genetic technology, and a result shows that rLaSota can serve as a live vector for stably expressing the S1 protein; secondly a recombinant NDV rLaSota/S1-HA expressing an HA gene is constructed, namely, the HA gene is inserted between P-M of the recombinant NDV rLaSota/S1; and thirdly immunogenicity assessment is performed. A result shows that the triple inactivated vaccine of the recombinant Newcastle disease, the bird flu and the infectious bronchitis, prepared through a conventional inactivated vaccine process has relatively good protection effects for the Newcastle disease, the bird flu and the infectious bronchitis.

The invention relates to a method for producing a combined vaccine, in particular to a method for producing a combined vaccine against Newcastle disease and infective bronchitis. The method comprises that: before culture in the same embryo, purity of breeding virus of the Newcastle disease and the infective bronchitis is improved; and during culturing in the same embryo, concentrations of two antigens in the combined vaccine against the Newcastle disease and the infective bronchitis are adjusted to reach reasonable combination. The vaccine prepared by the method is vaccinated with chicken flocks to generate ND and IB immunity without any interference. Furthermore, the vaccine strains are reasonably matched to effectively solve the problem of serious interference in simultaneous vaccination of the Newcastle disease living vaccine and infective bronchitis living vaccine, improve immunization quality and reduce the number of immunization.

The invention relates to a traditional Chinese medicine for curing avian influenza and newcastle disease. The prescription of the traditional Chinese medicine comprises the following materials: 6kg of radix astragali, 6kg of baicalensis, 6kg of goldthread roots, 6kg of flos lonicerae, 6kg of red paeonia, 6kg of liquorice, 6kg of ordate houttuynia, 7kg of Chinese pulsatilla, 7kg of dried rhizome of rehmannia, 7kg of tuber pinellia, 7kg of radix bupleuri, 7kg of loquat leaves, 8kg of forsythia, 8kg of dyers woad leaves and 8kg of isatis roots. The preparation method comprises the following steps: proportionally airing the traditional Chinese medicines; combing together and blending; and using a fodder pulverizer to pulverize into medicine powder which is put in a vat for spare use. The use method of the medicine is as follows: taking and pouring 1kg of prepared traditional Chinese medicine powder into 250kg of chicken fodder and blending uniformly for whole-day feeding which continues for 5 days to 7 days, and 7 days are used as a course of treatment.

The invention is concerned with a kind of bacterin to prevent fowl infectious disease, relating to a kind of bacterin preparation method to prevent Newcastle Disease and infectious bronchitis. The selected genus is Newcastle Disease virus La Sota individual plant, infectious bronchitis virus M41 individual plant and HN99 kidney type of aberrance individual plant. Dilute two kind of virus and inoculate in chick embryo allantoic cavity, collect liquid of embryo to alive and dead embryos as seed. Dilute and inoculate in allantoic cavity of affectable chick embryo to get liquid of virus embryo. Concentrate with equipment and add with formaldehyde solution to prepare bacterin through emulsion process. This invention can prevent Newcastle Disease, chick kidney type and breathing type infectious bronchitis, and it can reduce the times of injection and the cost of epidemic prevention for easy using and practicality.

The invention provides a preparation method of a recombined new castle disease virus vaccine strain which expresses African swine fever virus p72 proteins and a recombined new castle disease virus vaccine strain. The method provided by the invention comprises the following steps: constructing a recombinant transcriptional plasmid which is inserted with a p72 gene of African swine fever virus (ASFV); constructing a transcriptional helper plasmid system; carrying out a contransfection for the transcriptional plasmids and the transcriptional helper plasmids into host cells BHK-21 which can be duplicated in new castle disease attenuate strains; and saving and obtaining the recombined virus stain. The vaccine strain for expressing the African swine fever virus p72 proteins provided by the invention has important preservation and strategy meaning in the aspect of animal epidemic disease prevention and control, and can be applied to the treatment and prevention of African swine fever virus.

The present invention relates to one kind of recombinant Newcastle disease Lasota low virulent vaccine strain expressing wild or mutant bird flu virus H5 subtype HA protein, and is especially recombinant Newcastle disease Lasota low virulent vaccine strains prLasota-H5wtHA and rLasota-H5mutHA. The present invention also discloses the preparation process of the recombinant Newcastle disease Lasota low virulent vaccine strain and the application of the recombinant Newcastle disease Lasota low virulent vaccine strain in preparing vaccine for preventing bird flu.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention discloses a green tea composite chicken feed additive capable of enhancing the immunity of chickens. The additive is prepared from summer and autumn tea, dandelion, folium cortex eucommiae, radix isatidis, gouttuynia cordata, liquorice and the like, and has multiple effects of resisting bacteria and viruses, enhancing the immunity of animals and the like. A feeding test shows that the additive can improve all immune indexes, such as the serum newcastle disease antibody titer, the serum immune globulins and immune organ indexes, of laying hens and meat chickens; meanwhile, compared with an antibiotic added experimental group, the breeding test has the advantage that the death rate of animals in a treatment group does not have an obvious difference. The green tea composite chicken feed can be used as an additive for healthy breeding of the laying hens and the meat chickens; the raw materials are readily available and low in cost; the green tea composite chicken feed additive has a wide market prospect and high social and economical benefits.

The invention discloses a Chinese medicinal herb preparation for poultry. The effective constituents of a Chinese medicinal herb product disclosed by the invention are made up of echinacea purpurea, honeysuckle, hypericum perforatum, glycyrrhiza, folium isatidis, platycodon grandiflorum, lophatherum gracile, mint and hypericin. The Chinese medicinal herb preparation disclosed by the invention can relieve the exterior syndrome with drugs pungent in flavor and cool in property and clear away heat and toxic materials; and the main therapeutical effects include strengthening vital Qi and eliminating pathogens, balancing Yin and Yang, and improving non-specific immunity of organisms. The Chinese medicinal herb preparation for poultry has significant therapeutical effect for depressed spirit of poultry, feed intake decrease, egg laying reduction, eyelid swelling of poultry caused by unknown viruses, respiratory inflammation, yellow-green dung, weight increment decline, soft-shelled eggs, and death rate increment, etc.; furthermore, the Chinese medicinal herb preparation for poultry can be utilized to prevent the diseases of untypical Newcastle disease and avian influenza etc. and treat the relevant mixed infection, secondary infection, untypical diseases and chronic cases; and finally, the Chinese medicinal herb preparation for poultry well promotes the recovery of production performance after the poultry is healed.

The invention discloses a primer pair and a kit for identifying duck adenovirus II type, wherein the nucleotide sequence of the primer pair is represented as the SEQ ID No.1-2. The primer pair is strong in specificity, can accurately and high-effectively identify whether a to-be-test sample contains the duck adenovirus II type or not, while other poultry viruses, comprising poultry adenovirus, parvovirus, Tembusu virus and Newcastle disease virus, cannot be amplified to form DNA fragments. The primer pair is high in sensitivity, can at least detect 0.2258 ng of sample DNA. The primer and the PCR detection kit are short in detection time, wherein the whole PCR process only lasts for about 2 h. The primer pair and the kit are simple and economical, are free of expensive experimental instrument and reagents, can be used for detecting whether ill ducks are infected with the virus or not timely, so that greater economic loss can be avoided by means of corresponding measures.

The invention relates to a novel gene sequence of a recombinant chicken Alpha interferon, constructs a recombinant expression vector thereof and belongs to a gene engineering biological product obtained by a molecular biology method. The gene sequence of a newly designed chicken Alpha interferon is recombined into a pPICZ Alpha-A vector and then is confirmed on the special position of a microzyme by an electric conversion mode. Besides, a pichia expression system is adopted to express a foreign gene, thus being beneficial to the commercial production of the chicken interferon. The protein expressed by a gene group after being diluted by 4365158.3 times can completely restrain the attraction of vesicular stomatitis virus of 100-1000TCID50. Compared with a natural chicken Alpha interferon, the novel gene sequence of a recombinant chicken Alpha interferon has a higher anti-virus effect; the anti-virus effect thereof is improved by 8 times. Test also detects that the protein expressed by the gene can restrain the proliferation of a newcastle disease virus and an avian influenza virus; besides, the effect of the interferon with high concentration is more remarkable.

The invention relates to a preparation method of newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder. The bigeminy yolk-antibody lyophilized powder is prepared by preparing vaccines by preparing strains with strong immunity, preparing high immunity eggs, conducting disinfection, acidizing, octanoic acid treatment, refined exaction purification treatment, preparation and freeze-drying. The newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized powder prepared by using the method is a preparation capable of simultaneously preparing the newcastle diseases and chicken infectious bursal diseases, clinically reduces manipulation strength and reduces raise cost. A newcastle diseases, infectious bursal disease bigeminy high immunity-yolk-antibody lyophilized preparation is convenient to transport and store, is long in saving time and can keep high valence of antibody.

The invention provides a traditional Chinese medicine composition for treating newcastle disease. The traditional Chinese medicine composition comprises baical skullcap root, pagoda tree flowers, gentian, honeysuckle flowers, giant knotweed rhizome, common bletilla pseudobulb, codonopsis pilosula, cape jasmine fruit, panax notoginseng, astragalus membranaceus, Chinese angelica, kudzuvine root, tall gastrodia tuber and liquorice root. The traditional Chinese medicine composition is effective in treatment, capable of treating both the symptoms and the root causes and strengthening the body resistance to eliminate pathogenic factors, high in recovery rate without any toxic or side effect and low in recurrence and cost, has no phytotoxicity and no drug tolerance, can improve immunity of organisms and the like in treating chicken suffering from the newcastle disease.

The present invention discloses a combination of Chinese medicines for poultry and the preparation method and application. Hypericum forsythia and bidens grass are distilled. And then distilled liquid and residua are collected. The residua and the residual liquid after distillation being added with a hepatitis grass and a climbing nightshade are decocted three times. The decoction liquid is combined and concentrated to a thick concentration. A 95 percent ethanol is diluted to alcohol concentration of 60 percent to 70 percent. The drug liquid after sedimentation, filtration and concentration added with 95 percent ethanol are transferred to alcohol concentration of 75 percent to 80 percent and then are sedimentated and filtrated. The filtrate is concentrated. The drug liquid added with distilled water is frozenly precipitated, filtrated and concentrated. The combination of Chinese medicines is produced out after a combination of the distilled liquid of the hypericum forsythia and the bidens and the decoction liquid of the hepatitis grass and the climbing nightshade are combined. The combination of Chinese medicines has a cure rate of about 90% for a viruses and bacterial infectious disease of poultry, can be used to prepare the animal drug for treating duck viral hepatitis and chicken newcastle disease and has little side effect, safe use and fast absorption.

The present invention relates to a preparation method of a triple inactivated vaccine for the prevention of H9 subtype newcastle disease, infectious bronchitis and avian influenza, which is characterized in that: the content of the using NDVLaSota stain virus liquid is 10 to 22 percent, the content of IBVM41 stain virus liquid is also 10 to 22 percent and the content of the AIVHL stain virus liquid is 8 to 15 percent; the present invention uses the concentration and inactivation methods; the white oil adjuvant and the immune auxiliaries of Tween, Span and aluminum stearate are added to prepare the triple inactivated vaccine; the present invention realizes the multiple vaccine prevention and achieves the purpose of prevention a plurality of diseases by one needle, which can be used for greatly developing the husbandry, so as to benefit the mankind and improve the living standard of the people.

The invention belongs to the technical field of molecular biology and particularly relates to NDV (Newcastle disease virus) recombinant virus expressing DHAV-1 and DHAV-3 VP1 genes.The recombinant virus is recorded as rLS-1VP1-2A-3VP1 and is obtained inserting serially connected DHAV-1 and DHAV-3 VP1 genes into an NDV vector and carrying out saving.The NDV (Lasota strain) is used as the vector for the recombinant virus, the serially connected DHAV-1 and DHAV-3 VP1 genes are inserted into the NDV (Lasota strain) to obtain a vector, and the DNV recombinant virus co-expressing DHAV-1 and DHAV-3 VP1 genes is obtained by determining an optimal insertion site to insert the DHAV VIP1 gene; the recombinant virus is useful in preventing duck hepatitis A viruses (type 1 and type 3) and duck Newcastle disease and filling the current blank of DHAV-3 vaccines.