15 results about "Newcastle disease" patented technology

The invention belongs to the technical field of biology, and discloses a TaqMan primer probe combination for detecting virulent and attenuated Newcastle disease, which comprises a virulent primer RT, a virulent probe RT-probe, a universal primer SRT and a universal probe SRT-probe, the virulent primer RT comprises an upstream primer RT-F and a downstream primer RT-R, and the universal primer SRT comprises an upstream primer SRT-F and a downstream primer SRT-R; a TaqMan probe fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for virulent and attenuated NDV (Newcastle Disease Virus) established by using the TaqMan primer probe combination has no cross reaction on amplification of common avian viruses AIV, FADV, IBV and IBDV and has good specificity; the lowest detection concentration of virulent and attenuated Newcastle disease virus is 101 copies / uL, which is 100 times higher than that of a conventional RT-PCR method, and the kit has good sensitivity; and compared with the conventional RT-PCR method, the detection rate is 100% and is superior to that of the conventional RT-PCR method, and the detection result is consistent with the sequencing result.

The application discloses a chicken Newcastle disease, avian influenza and chicken infectious rhinitis triple inactivated vaccine and preparation and application thereof, and relates to the technical field of bioengineering. The application discloses a preparation method and application of a chicken Newcastle disease, avian influenza and chicken infectious rhinitis (A, B and C three serotypes) subunit protein triple inactivated vaccine. The preparation method comprises the following steps: constructing and expressing a serum B paracolon avian bacillus subunit protein expression strain; and preparing the chicken Newcastle disease, H9 subtype avian influenza and chicken infectious rhinitis (A, B and C three serotypes) subunit protein triple inactivated vaccine at a certain ratio. The triple inactivated vaccine has good safety, can be used for the prevention of chicken Newcastle disease, H9 subtype avian influenza and A, B and C three serotypes paracolon avian bacillus diseases in a laying hen group, and does not cause the decrease of the laying rate after immunization. Meanwhile, the paracolon avian bacillus subunit protein can improve the antibody level of the Newcastle disease and the H9 avian influenza, and the protection of the vaccine on the A, B and C three serotypes paracolon avian bacillus can reach 100%.

Described herein are recombinant Newcastle disease viruses ("NDVs") comprising a packaged genome, wherein the packaged genome comprises a transgene comprising a nucleotide sequence encoding a protein comprising a SARS-CoV-2 delta variant spike protein or portion thereof. Also described herein are recombinant NDVs comprising a packaged genome, wherein the packaged genome comprises a transgene encoding a chimeric F protein, wherein the chimeric F protein comprises a SARS-CoV-2 delta variant spike protein ectodomain and NOV F protein transmembrane and cytoplasmic domains. The recombinant NDVs and compositions thereof are useful for the immunizing against SARS- CoV-2 delta variant as well as the prevention of COVID-19.

Described herein are recombinant Newcastle disease viruses ("NDVs") comprising a packaged genome, wherein the packaged genome comprises a transgene comprising a nucleotide sequence encoding a protein comprising a SARS-CoV-2 spike protein or portion thereof. Also described herein are recombinant NDVs comprising a packaged genome, wherein the packaged genome comprises a transgene encoding a chimeric F protein, wherein the chimeric F protein comprises a SARS-CoV-2 spike protein ectodomain and NOV F protein transmembrane and cytoplasmic domains. Further, described herein are immunogenic compositions comprising a recombinant NDV(s). The recombinant NDVs and immunogenic compositions are useful for the immunizing against SARS-CoV-2 as well as the prevention of CO VID-19.

The invention discloses a primer probe composition for detecting AIV, NDV virulent strains and IBV, a kit and application, and belongs to the technical field of fluorescence detection. The sequence of the primer probe combination is shown as SEQ ID NO.1-SEQ ID NO.9, the primer probe combination comprises a primer pair and a probe for detecting the avian influenza virus, the Newcastle disease virus virulent strain and the avian infectious bronchitis virus, and the primer probe combination can be used for detecting and distinguishing all subtypes of the avian influenza virus and the Newcastle disease virus virulent strain and different genotypes of the avian infectious bronchitis viruses. The kit is high in specificity and sensitivity, does not have cross reaction with other common viruses, and is high in detection sensitivity, and the minimum detectable quantity of the three viruses is 0.8 copy, so that the specific, sensitive and rapid detection of AIV, NDV and IBV can be realized, and the defects of the conventional detection technology are overcome. The method has important practical significance on timely blocking epidemic situation propagation, reducing breeding loss, guaranteeing public health safety and the like.

This invention discloses a primer composition, kit, and application for avian pathogens. The primer composition comprises a crRNA with the sequence shown in SEQ ID NO: 1, an RT-RAA upstream primer with the sequence shown in SEQ ID NO: 2, and an RT-RAA downstream primer with the nucleotide sequence shown in SEQ ID NO: 3. It may also include a fluorescent probe with the sequence TTTTT. This primer composition can be used for the detection of avian infectious bronchitis virus, exhibiting high specificity and no cross-reactivity with common avian viruses such as avian influenza virus, Newcastle disease virus, and infectious laryngotracheitis virus. Furthermore, the primer composition has high sensitivity, with a detection limit as low as 4.25 × 10⁻⁶. 2 The primer composition exhibits high copy number / μL, good reproducibility, and stable results. It is suitable for single-tube isothermal nucleic acid amplification detection, offering mild reaction conditions and significantly reduced detection time, demonstrating excellent industrial application value.

The present invention provides recombinant Newcastle disease virus (NDV) viral vectors comprising heterologous polynucleotides encoding avian influenza antigens. The recombinant viral vectors are suitable for use in immunogenic compositions and vaccines and are capable of providing protection against avian influenza and other pathogens when administered to avians.

The invention belongs to the technical field of biology, and discloses construction and application of a recombinant herpesvirus of turkey for co-expressing an HA gene and an F gene, and the recombinant herpesvirus of turkey for co-expressing the HA gene and the F gene is characterized in that a target gene 1 and a target gene 2 are respectively inserted between an HVT053 site and an HVT054 site and between an HVT065 site and an HVT066 site in a UL region of the herpesvirus of turkey; or respectively inserting the target gene 1 and the target gene 2 between the HVT053 site and the HVT054 site and between the HVT087 site and the HVT088 site in the UL region of the turkey herpesvirus; and respectively obtaining a recombinant virus 1 and a recombinant virus 2. The two recombinant turkey herpesviruses can grow, replicate and stably inherit in vitro, and a good immune protection effect is provided for H9N2 subtype avian influenza and Newcastle disease. Meanwhile, the invention also provides application and a vaccine of the recombinant herpesvirus of turkeys.

A bacteria-based compound for the prevention and treatment of a number of strains of avian influenza via oral intake by way of feed or drinking water or both. The agent is an inventive treatment compound comprising a bacterial-based culture which is directed to the prevention and treatment of avian influenza primarily for use in commercial birds such as poultry. The inventive treatment compound disclosed herein is produced from a lipopolysaccharide of a Gram-negative bacterial strain of the Brevundimonas group. The compound may be delivered to poultry by way of liquid or dry feed according to an effective prescribed regimen. A similar treatment approach may be taken as well after the bird is infected. Use of the composition is effective in the prevention and treatment of a variety of avian influenza, including high pathogenicity avian influenza, low pathogenicity avian influenza, Newcastle disease virus, infectious bronchitis disease virus, and infectious laryngotracheitis virus.

The invention discloses a primer probe composition for detecting H9 subtype AIV, NDV and IBV, a kit and application, and belongs to the technical field of fluorescence detection. The sequence of the primer probe combination is shown as SEQ ID NO.1-SEQ ID NO.9, the primer probe combination comprises a primer pair and a probe for detecting the H9 subtype avian influenza virus, the Newcastle disease virus and the infectious bronchitis virus, can detect and distinguish epidemic H9 viruses of different branches, NDV of different genotypes and IBV of different genotypes, does not have cross reaction with other common viruses, and can be used for detecting and distinguishing the H9 viruses of different branches, the NDV of different genotypes and the IBV of different genotypes. The detection sensitivity is high, and the minimum detectable quantity of the three viruses is 0.8 copy, so that the specific, sensitive and rapid detection of H9, NDV and IBV can be realized, and the defects of the existing traditional detection technology are overcome. The method has important practical significance on timely blocking epidemic situation propagation, reducing breeding loss, guaranteeing public health safety and the like.

The invention discloses a preparation method of a small peptide zinc feed additive for improving immunity of broiler chickens, and relates to the technical field of feed additives.The method comprises the steps that soybean protein isolate and casein are mixed and then subjected to primary enzymolysis through pepsin, and then synergistic enzymolysis is conducted through neutral protease and flavourzyme; and carrying out ultrafiltration to obtain a small peptide component with the molecular weight of less than 1000 Daltons, chelating the small peptide component with zinc sulfate heptahydrate according to a molar ratio of 1: 2, carrying out spray drying to obtain a finished product, finally carrying out quality detection on the finished product, and carrying out sealing and dark preservation to obtain a qualified product. The invention aims to solve the problems of uncontrollable immune active components, insufficient efficacy verification and non-environment-friendly process in the existing feed additive, and can directionally enrich Arg-Gly-Asp, Lys-Pro and other immune active peptide fragments to form a high-stability small peptide zinc chelate, significantly improve the antibody titer, serum IgG level and intestinal barrier function of the broiler Newcastle disease, and improve the immunity of the broiler Newcastle disease. Meanwhile, the process is clean, low in cost and suitable for industrial production.

The application discloses an African green monkey kidney cell clone and application thereof in detection of chicken newcastle disease virus, and particularly relates to the technical field of biological product detection. The detection method specifically comprises the following steps: (1) cell plating: taking prepared VERO-K6 cell suspension, taking a certain amount of the cell suspension into a dish, and then adding the cell suspension into a cell culture plate by using a cell plating gun; the cell culture plate is placed into a culture box and cultured until the cells grow into a dense monolayer; (2) sample dilution: taking a certain amount of chicken newcastle disease virus liquid into a 10 ‑1 EP tube, oscillating and uniformly mixing the virus liquid, taking an equal amount of virus liquid from a 10 ‑1 centrifugal tube into a 10 ‑2 centrifugal tube, and sequentially performing 10 times dilution; (3) sample adding; and (4) culture: placing the cell culture plate into the culture box, culturing, recording the number of effective lesion holes, and calculating TCID 50 according to the Reed-Muench method. Compared with the existing chicken newcastle disease virus detection method, the application has the advantages of low detection cost, simple detection process, short detection period and the like.

This invention relates to the fields of veterinary immunology and molecular biology, and discloses a chicken cytokine composition with immune-enhancing effects and its applications. The immune-enhancing chicken cytokine composition includes recombinant chicken cytokines IL-9 and IL-21, whose amino acid sequences are shown in SEQ ID NO.4 and SEQ ID NO.8, respectively. These two cytokines can upregulate the expression of IL-1β, IL-2, IL-4, IL-6, IFN-γ, IL-17A, and TNF-α in chicken PBMCs, stimulating Th1, Th2, and Th17 immune responses in chickens. When used in combination with a Newcastle disease-avian influenza bivalent inactivated vaccine, this recombinant chicken cytokine composition exhibits a significant immune-enhancing effect and has potential application prospects. This chicken cytokine composition has advantages such as ease of preparation, high yield, good stability, and low production cost.

Described herein are recombinant Newcastle disease viruses ("NDVs") comprising a packaged genome, wherein the packaged genome comprises a transgene comprising a nucleotide sequence encoding a protein comprising a SARS-CoV-2 Omicron variant spike protein or portion thereof, or a derivative thereof. Also described herein are recombinant NDVs comprising a packaged genome, wherein the packaged genome comprises a transgene encoding a chimeric F protein, wherein the chimeric F protein comprises a SARS-CoV-2 Omicron variant spike protein ectodomain or a derivative thereof, and NDV F protein transmembrane and cytoplasmic domains. Further, described herein are immunogenic compositions comprising a recombinant NDV(s). The recombinant NDVs and immunogenic compositions are useful for the immunizing against SARS-CoV-2 as well as the prevention of COVID-19.

The invention relates to the technical field of medicines, in particular to application of NDV in preparation of an AKT inhibitor synergist. The invention provides an application of NDV (Newcastle Disease Virus) in preparation of an AKT inhibitor synergist. The invention discloses a synergistic effect of the NDV and AKT inhibitor in treatment of hepatocellular carcinoma through in-vivo and in-vitro experiments for the first time. Experimental results show that the NDV can significantly enhance the killing ability of the AKT inhibitor to liver cancer cells, effectively inhibit the growth of tumor cells and induce apoptosis. According to the combined treatment scheme, the limitation that rapid drug resistance is easy to generate when an AKT inhibitor is singly used is overcome, the anti-tumor effect is further enhanced through the immune activation effect of the oncolytic virus, and a novel drug combination strategy with a synergistic mechanism is provided for treatment of hepatocellular carcinoma.