3 results about "Newcastle disease" patented technology

This invention discloses a primer composition, kit, and application for avian pathogens. The primer composition comprises a crRNA with the sequence shown in SEQ ID NO: 1, an RT-RAA upstream primer with the sequence shown in SEQ ID NO: 2, and an RT-RAA downstream primer with the nucleotide sequence shown in SEQ ID NO: 3. It may also include a fluorescent probe with the sequence TTTTT. This primer composition can be used for the detection of avian infectious bronchitis virus, exhibiting high specificity and no cross-reactivity with common avian viruses such as avian influenza virus, Newcastle disease virus, and infectious laryngotracheitis virus. Furthermore, the primer composition has high sensitivity, with a detection limit as low as 4.25 × 10⁻⁶. 2 The primer composition exhibits high copy number / μL, good reproducibility, and stable results. It is suitable for single-tube isothermal nucleic acid amplification detection, offering mild reaction conditions and significantly reduced detection time, demonstrating excellent industrial application value.

The application discloses an African green monkey kidney cell clone and application thereof in detection of chicken newcastle disease virus, and particularly relates to the technical field of biological product detection. The detection method specifically comprises the following steps: (1) cell plating: taking prepared VERO-K6 cell suspension, taking a certain amount of the cell suspension into a dish, and then adding the cell suspension into a cell culture plate by using a cell plating gun; the cell culture plate is placed into a culture box and cultured until the cells grow into a dense monolayer; (2) sample dilution: taking a certain amount of chicken newcastle disease virus liquid into a 10 ‑1 EP tube, oscillating and uniformly mixing the virus liquid, taking an equal amount of virus liquid from a 10 ‑1 centrifugal tube into a 10 ‑2 centrifugal tube, and sequentially performing 10 times dilution; (3) sample adding; and (4) culture: placing the cell culture plate into the culture box, culturing, recording the number of effective lesion holes, and calculating TCID 50 according to the Reed-Muench method. Compared with the existing chicken newcastle disease virus detection method, the application has the advantages of low detection cost, simple detection process, short detection period and the like.

This invention relates to the fields of veterinary immunology and molecular biology, and discloses a chicken cytokine composition with immune-enhancing effects and its applications. The immune-enhancing chicken cytokine composition includes recombinant chicken cytokines IL-9 and IL-21, whose amino acid sequences are shown in SEQ ID NO.4 and SEQ ID NO.8, respectively. These two cytokines can upregulate the expression of IL-1β, IL-2, IL-4, IL-6, IFN-γ, IL-17A, and TNF-α in chicken PBMCs, stimulating Th1, Th2, and Th17 immune responses in chickens. When used in combination with a Newcastle disease-avian influenza bivalent inactivated vaccine, this recombinant chicken cytokine composition exhibits a significant immune-enhancing effect and has potential application prospects. This chicken cytokine composition has advantages such as ease of preparation, high yield, good stability, and low production cost.