The invention belongs to the technical field of preparation of vaccines for poultry, and particularly relates to a preparation method of a triple vaccine for treating avian H3N8 influenza
virus, which comprises the following steps: step 1,
virus culture: respectively inoculating the H3N8 influenza
virus and
Newcastle disease virus into
chick embryos, respectively harvesting chick
embryo liquids of the H3N8 influenza virus and the
Newcastle disease virus after culture, and respectively adding the H3N8 influenza virus and the
Newcastle disease virus into the chick
embryo liquids of the H3N8 influenza virus and the Newcastle
disease virus; and then carrying out
ultrafiltration concentration to obtain an H3N8 influenza virus
stock solution and a Newcastle
disease virus
stock solution, and inoculating the LMH cells with the
fowl adenovirus. The H3N8 influenza virus triple vaccine can improve the epidemic prevention efficiency of poultry, reduce the epidemic prevention cost, reduce the stress reaction and prevent various diseases, can prevent three poultry diseases at the same time through one-time
inoculation, can ensure that the poultry obtains comprehensive
immune protection in a short time, reduces the
vaccination times, and improves the immunization efficiency of the poultry. The stress reaction of poultry caused by
vaccination can be reduced, the healthy growth of the poultry is facilitated, and meanwhile, the
workload of farmers is reduced.