24 results about "Poultry disease" patented technology

The invention relates to the technical field of knowledge graph construction, in particular to a poultry disease knowledge graph construction method, which comprises the following steps of S1, defining a top layer ontology framework; s2, performing term merging on the multi-source poultry disease data to generate a standardized term set; s3, constructing an environment-pathogen-host chain type triple based on the ontology framework and the standardized term set; s4, performing space-time alignment and conflict resolution on the chain triad in the S3, and outputting a fusion knowledge base; s5, extracting a triple change sequence of continuous time slices based on the fusion knowledge base in the S4, and generating a dynamic propagation chain; and S6, executing logic verification and backtracking verification on the dynamic propagation chain in the S5, and outputting a finally constructed poultry disease knowledge graph. According to the method, through multi-source data standardization, dynamic propagation modeling and logic verification fusion, structured, dynamic and credible expression of the poultry disease propagation path is realized, and a complete and reliable knowledge support is provided for accurate prevention and control.

The invention discloses a management task generation method for livestock and poultry health intelligent monitoring based on multi-modal sensing, and relates to the technical field of agricultural informationization, and the method comprises the steps: building a disease causal network of a livestock and poultry breeding type, and generating a known disease multi-label time-varying vector of each stage of the livestock and poultry breeding type; establishing a multi-modal behavior encoder of the livestock and poultry breeding type, extracting each modal behavior vector of the real-time target livestock and poultry breeding type and the known disease multi-label time-varying vector of each stage of the livestock and poultry breeding type for correlation matching screening, and obtaining a concerned known disease set of the real-time livestock and poultry breeding type; and analyzing the lethal risk probability of each concerned known disease of the bred livestock and poultry types in unit time, screening the concerned known disease lethal risk probability and a known disease treatment task library, establishing a livestock and poultry disease treatment resource constraint function, and generating a priority sequence of the concerned known disease initial treatment tasks of the real-time bred livestock and poultry types. The death rate and economic loss are reduced, and the intelligent management level of the farm is remarkably improved.

The application discloses a sweet wormwood extract, a preparation method and application thereof and a sweet wormwood extract veterinary drug, and the method comprises the following steps: S1, heating and extracting sweet wormwood byproducts with an extraction solvent, filtering, discarding the filtrate, and obtaining an intermediate product filter cake; S2, concentrating the intermediate product filter cake to a constant weight; S3, heating and extracting the constant weight obtained in the step S2 with deionized water, filtering, and taking the filtrate; S4, concentrating the filtrate obtained in the step S3 to a constant weight, and obtaining the sweet wormwood extract. The application provides a sweet wormwood extract and a preparation method thereof, and provides a veterinary drug based on the sweet wormwood extract; the veterinary drug obtained by the application can clear heat, resolve summer-heat, and relieve deficiency heat, and can be used for treating various livestock and poultry diseases; the preparation method is simple, easy to operate, raw materials are easy to obtain, production cost is low, and the application has a good market application prospect.

The invention belongs to the technical field of livestock and poultry epidemic disease detection, and provides a portable blood sample collection device for livestock and poultry epidemic disease detection, which comprises a box body, one side of the box body is rotatably provided with a box door, the inner walls of the two sides of the box body are both provided with a plurality of groups of transverse plates, and adjusting assemblies are arranged between the transverse plates and the inner walls of the box body. A mounting plate is arranged between every two groups of transverse plates, guide rails are arranged on the sides, close to the mounting plates, of the transverse plates, guide blocks slidably connected to the guide rails are fixedly connected to the two sides of the mounting plates, a plurality of groups of placing grooves for placing test tubes are formed in the tops of the mounting plates in a penetrating manner, and clamping assemblies are arranged in the placing grooves; moving assemblies are arranged at four corners of the bottom of the box body; according to the device, test tubes with different lengths and diameters can be clamped and fixed through the adjusting assembly and the clamping assembly, the application range is effectively widened, the device can be moved more conveniently, and meanwhile, the device can be rapidly fixed after being moved.

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The utility model discloses an anti-splashing device for poultry drinking water, and relates to the technical field of poultry drinking water. The utility model comprises: a base plate; the support is fixedly installed at the top of the bottom plate, a movable plate is installed on the support in a sliding mode, a splash-proof cover is fixedly installed at the bottom of the movable plate, and a water tank is fixedly installed between the two sides of the inner wall of the splash-proof cover. Through cooperation of the support, the movable plate, the splash-proof cover, the water tank, the adjusting assembly and the movable assembly, the normal drinking water requirement of poultry is met, meanwhile, the splashing phenomenon in the drinking water process is effectively prevented, waste of water resources is reduced, the humidity of the breeding environment is reduced, breeding of harmful microorganisms such as bacteria and molds is reduced, and the breeding quality is improved. The poultry disease probability is reduced, healthy growth of poultry is guaranteed, meanwhile, corrosion to breeding equipment is reduced, the service life of the equipment is prolonged, and breeding benefits are improved.

The application belongs to the field of biology, and discloses a method for high-throughput screening of viral CTL epitopes based on immunopeptidomics, which comprises the following steps: transfecting mammalian cells with eukaryotic expression plasmids having MHC I molecules in series with the alpha chain and the beta2m chain to establish a mammalian cell line expressing animal MHC I molecules; using a virus to infect the mammalian cell line expressing MHC I molecules to prepare MHC I-peptide complexes; and obtaining antigen peptides existing in the MHC I-peptide complexes. The method takes chicken MHC I allele BF2*1901 molecules as the research object, takes H9N2 subtype avian influenza virus as the model virus, comprehensively characterizes chicken MHC I restricted H9N2 antigen peptide groups, and identifies immunodominant CTL epitope immunity, and is suitable for CTL epitope screening of different chicken MHC I molecules and different subtypes of avian influenza viruses, provides a fast, efficient and economical technical means for studying the specific binding of chicken MHC I molecules and antigen peptides, provides a favorable reference for T cell epitope screening of major animal pathogens, and provides a scientific basis for poultry disease-resistant breeding, development of new vaccines and immune evaluation strategies. Meanwhile, the application also discloses restricted epitope peptides, nucleic acid molecules and applications.

The invention belongs to the technical field of poultry disease detection, and particularly relates to a poultry immunosuppressive disease joint detection method based on multiple fluorescent quantitative PCR (Polymerase Chain Reaction). The method sequentially comprises the following steps: determining a detection target, designing a specific primer and a probe, preparing a positive standard substance, pre-treating a sample, extracting nucleic acid, purifying a nucleic acid sample, establishing a multiple fluorescent quantitative PCR reaction system, setting a multiple fluorescent quantitative PCR reaction program, amplifying, and analyzing and judging a result. On the basis, by designing a multiplex fluorescent quantitative PCR detection system and the specific primer probe, the kit has the advantages that synchronous detection of multiple poultry immunosuppressive pathogens is realized, multiple independent detection on the same sample is not needed, the operation process is simplified, the detection cost is reduced, and the waste of the sample is reduced; the problems that a traditional single pathogen detection method is low in efficiency and high in cost are solved.

The invention discloses a livestock and poultry disease root cause diagnosis and intervention recommendation method, relates to the technical field of livestock and poultry disease diagnosis, and solves the problem that root cause diagnosis is difficult to realize in the prior art. According to the embodiment of the invention, the disease occurrence of the individual livestock and poultry is regarded as the result of the multi-agent dynamic game, and the root cause back-stepping is carried out by utilizing the inverse reinforcement learning, so that the root cause diagnosis result and the intervention recommendation suggestion are obtained, decision support for direct action is provided, and the management intelligence degree is greatly improved. Meanwhile, the game reasoning model comprehensively considers the effects of environment, group behaviors and biological pathogens, avoids the one-sidedness of single factor diagnosis, and is more scientific and reliable in conclusion.

The invention discloses a preparation method and application of an oral chicken infectious anemia vaccine. The preparation method comprises the following steps: providing an eimeria vector; genes of VP1 protein and VP2 protein for coding the chicken infectious anemia virus are introduced into the eimeria vector; after oral immunization, the recombinant eimeria can effectively reduce the clinical symptoms and virus load of chicken lean transmission, saves the manpower and material resources of vaccine immunization, is suitable for prevention and control of poultry epidemic diseases in large-scale breeding industry, and has a wide application prospect.

This invention discloses a chicken infectious bronchitis virus (IBBV) nanoparticle, its preparation method, and its application, belonging to the field of poultry vaccine technology. The nanoparticles are formed by the self-assembly of an SRBD-SpyTag fusion protein and a Ferritin-SpyCatcher fusion protein via a SpyTag / SpyCatcher covalent linkage system. The SRBD-SpyTag fusion protein is formed by fusing a SpyTag sequence to the N-terminus or C-terminus of the S1 protein receptor-binding domain of IBV. The nanovaccine prepared from these nanoparticles exhibits high safety, rapid antigen module replacement, and is suitable for preventing IBV infection. The technical solution of this invention has advantages such as structural stability, strong immunogenicity, and rapid iteration, providing a novel vaccine solution for poultry disease control.

The invention discloses a chlamydia psittaci liquid-phase probe targeted capturing and library building method and a kit thereof, and belongs to the technical field of virus detection, a specific probe is designed to enrich pathogen DNA in a targeted manner, a sample treatment process is optimized, targeted enrichment and high-throughput sequencing are combined, and the method has the advantages of wide coverage, good flexibility, high sensitivity, high accuracy and the like. The method has the characteristics of high sensitivity, high specificity and the like, and can be used for quantitative, qualitative and deep analysis and other multi-dimensional information output. By designing the specific probe and optimizing the sample treatment and library building process, the sensitivity, specificity and efficiency of chlamydia psittaci detection are remarkably improved, and the blank in the aspects of low-load detection, whole-genome analysis and high-throughput adaptation in the prior art is filled; the method has important application value in the fields of clinical diagnosis, poultry epidemic disease prevention and control and public health safety.

The invention provides a poultry disease diagnosis method and device based on symptom grading and rule reasoning, and the method comprises the steps: determining poultry disease symptoms and a poultry disease symptom grading knowledge base which comprises the information of a plurality of standard poultry disease symptoms, the information of each standard poultry disease symptom comprises a grade of the standard poultry disease symptom and a poultry disease associated with the standard poultry disease symptom, and carrying out semantic matching on the poultry disease symptom and each standard poultry disease symptom in a poultry disease symptom grade knowledge base, obtaining candidate poultry disease symptoms matched with the poultry disease symptoms, grades of the candidate poultry disease symptoms and matching confidence coefficients, performing rule reasoning based on the candidate poultry disease symptoms, the grades of the candidate poultry disease symptoms and the matching confidence coefficients in combination with a poultry disease diagnosis scoring function, and determining scores of candidate poultry diseases, and determining a poultry disease diagnosis result based on the score of the candidate poultry disease.

The invention belongs to the technical field of preparation of vaccines for poultry, and particularly relates to a preparation method of a triple vaccine for treating avian H3N8 influenza virus, which comprises the following steps: step 1, virus culture: respectively inoculating the H3N8 influenza virus and Newcastle disease virus into chick embryos, respectively harvesting chick embryo liquids of the H3N8 influenza virus and the Newcastle disease virus after culture, and respectively adding the H3N8 influenza virus and the Newcastle disease virus into the chick embryo liquids of the H3N8 influenza virus and the Newcastle disease virus; and then carrying out ultrafiltration concentration to obtain an H3N8 influenza virus stock solution and a Newcastle disease virus stock solution, and inoculating the LMH cells with the fowl adenovirus. The H3N8 influenza virus triple vaccine can improve the epidemic prevention efficiency of poultry, reduce the epidemic prevention cost, reduce the stress reaction and prevent various diseases, can prevent three poultry diseases at the same time through one-time inoculation, can ensure that the poultry obtains comprehensive immune protection in a short time, reduces the vaccination times, and improves the immunization efficiency of the poultry. The stress reaction of poultry caused by vaccination can be reduced, the healthy growth of the poultry is facilitated, and meanwhile, the workload of farmers is reduced.

The poultry disease vaccine diluting device for animal medicine comprises a device body, a liquid inlet and an indicator light, the top of the device body is connected with the liquid inlet in a penetrating mode, a touch screen is connected to the front face of the device body in an embedded mode, and a pressure displayer is connected to the bottom of one side of the front face of the device body in an embedded mode. An indicating lamp is fixedly connected to the bottom of the other side of the front of the device main body, a waste liquid collecting tank is fixedly connected to the bottom in the device main body, a mixing bin is fixedly connected to one side of the middle part in the device main body, a magnetic stirrer is arranged in the mixing bin, and the rotating speed of the magnetic stirrer can be adjusted between 50 rpm and 300 rpm. When a vaccine stock solution and a diluent enter the mixing bin, the magnetic stirrer starts to work, the stirrer is driven by magnetic force to rotate, so that the vaccine stock solution and the diluent are fully mixed, the inner wall of the mixing bin is made of a medical grade 316L stainless steel material, the material has good corrosion resistance and biocompatibility, and it is ensured that impurities or pollutants cannot be introduced in the mixing process.

The present application relates to poultry disease immune control technology, and in particular to a duck circovirus genetic engineering subunit vaccine and a preparation method and application thereof. A primer group shown as SEQ ID NO. 1 and SEQ ID NO. 2 is used to perform PCR amplification on a target Cap gene with a nuclear localization signal removed, a prokaryotic expression vector pET28-cap containing a His tag is constructed, is transferred into a BL21 (DE3) competent cell for induction expression, and the cap protein is purified by using a NI purification resin. After denatured cap protein after purification is renatured, the antigen can be obtained, is emulsified with white oil adjuvant to prepare a vaccine. The present application successfully realizes prokaryotic expression of the cap protein by designing primers of the Cap gene with the nuclear localization signal removed, and shows good immunogenicity, and can be used for development of duck circovirus genetic engineering subunit vaccine, egg yolk antibody and the like.

The invention discloses a method for analyzing the influence of salmonella infection on chicken liver transcriptome RNA m6A modification, and relates to the technical field of bioengineering, and the method comprises the following steps: animal model establishment and sample collection, RNA extraction, sequencing and data quality control, bioinformatics analysis and differential m6A peaks identification, and data integration and function enrichment analysis. By integrating MeRIP-seq and a transcriptome sequencing technology, a dynamic regulation and control mechanism of RNA m6A modification in the process of infecting the chicken liver with salmonella is disclosed, an m6A modification and gene expression association analysis framework under an infection model is constructed, the analysis depth of an epigenetic regulation and control mechanism of poultry immune response is remarkably improved, and the application prospect is broad. And by establishing a standardized experimental process and a bioinformatics analysis system, accurate identification of differential modification sites and functional association thereof from a transcriptome level is realized, and a new technical path is provided for poultry disease resistance research.

The application discloses application of chicken type IV interferon ChIFN-v in antiviral, and belongs to the technical field of biology.The amino acid sequence of the chicken type IV interferon ChIFN-v is shown as SEQ ID NO.4.The application provides evidence of existence of the chicken type IV interferon ChIFN-v, and proves that the chicken type IV interferon ChIFN-v has strong antiviral activity through in-vitro cell protection experiments and animal infection treatment experiments.The application also verifies the synergistic effect of the ChIFN-v protein and the chicken type I interferon ChIFN-alpha in antiviral, provides new drug resources and solid data support for prevention and control of poultry diseases, has a clear commercial transformation path, and has remarkable social and economic benefits and wide market prospect.

The application belongs to the technical field of biological genetic engineering, and relates to application of PRMT7 as a target point in preparation of a medicine for inhibiting proliferation of subgroup J avian leukemia virus, and application of a PRMT7 gene polymorphism site as a molecular marker for evaluating resistance of poultry to subgroup J avian leukemia virus. Research shows that PRMT7 is involved in immune regulation of a host to infection of subgroup J avian leukemia virus, and inhibition of PRMT7 expression can enhance activity of a type I interferon related path and reduce a virus replication level. Further, a plurality of nucleotide polymorphism sites in the PRMT7 gene are screened to obtain a significant correlation with an ALV-J related phenotype, and the related phenotype includes a pancreas tissue index, a spleen tissue index, a bursa of fabricius tissue index, an IL-6 level and an IFN alpha level. The application provides a new technical scheme for prevention and control of subgroup J avian leukemia virus, development of an antiviral target point and poultry disease resistance molecular breeding.

This invention discloses a Riemerella anticipestifer phage SD-RA1, its isolation method, and its applications. This phage can simultaneously lyse serotypes 1 and 7 of Riemerella anticipestifer. It was deposited at the China Center for Type Culture Collection (CCTCC) on August 23, 2021, with accession number CCTCC M 20211061. This phage can be used not only to prepare drugs for the prevention and treatment of various livestock and poultry diseases caused by Riemerella anticipestifer infection, but also to prepare livestock and poultry feed additives and environmental disinfectants. The phage and its compositions are safe to use and have no side effects, effectively avoiding antibiotic residues and the problem of inducing drug-resistant Riemerella anticipestifer caused by traditional antibiotic use.

The utility model relates to the field of rural cultivation, in particular to a feeder capable of monitoring, marking and preventing poultry diseases. The feeder comprises a feed barrel, a base, a horizontal supporting plate, a chassis, a partition ring, a barrel cover, a feed barrel handle and the like. The 360-degree rotation design is adopted, monitoring is comprehensive, a disinfection reagent is pumped out of the disinfection water tank through a water suction pump, the reagent is sprayed out in a mist shape through a spray head, poultry coming to eat is disinfected and sterilized, a rotating disc is arranged above the disinfection water tank, and a spray gun above the rotating disc and a thermodetector can rotate by 360 degrees; the hydraulic motor is in a power-off state all the time when the temperature measuring instrument above the rotary table does not monitor the poultry with the high temperature, and when the temperature measuring instrument monitors the poultry with the high temperature, the hydraulic motor sends the marking solution from the marking solution box to the spray gun through control, and the marking solution is ejected out of the spray gun to mark the poultry in a small range in front of the temperature measuring instrument. And a farmer can conveniently and subsequently find the poultry with high body temperature.

The invention discloses a progressive knowledge enhancement-based large language model question and answer method in the field of poultry. The method comprises the following steps of: firstly, disassembling a complex question of a user into a sub-question set through a fine-tuning planning large language model; a hierarchical matching strategy is adopted, questions and sub-questions are subjected to vectorization through a field adaptation embedding model and then matched with a field knowledge base, when the similarity is larger than or equal to a threshold value, answers are directly returned, otherwise, the sub-questions are analyzed through a triple extraction model to generate standardized triads, missing triads are complemented through a poultry disease knowledge graph, and a poultry disease knowledge graph is obtained. If the atlas cannot be completed, activating a webpage retrieval module to perform information extraction and structured completion; performing feature alignment and consistency verification by fusing the question and answer large language model with multi-source knowledge to generate a final answer; dynamic knowledge evolution is synchronously realized, a high-confidence triple of webpage retrieval is filtered and then writes back to the knowledge graph, and when the user satisfaction reaches a threshold, question and answer pairs are written back to the domain knowledge base. The question and answer ability of the large language model in the poultry field is significantly improved.

This invention relates to the field of image processing technology and discloses a multimodal image fusion method based on Mamba and attention mechanisms. This method significantly improves the multimodal image fusion effect through the collaborative work of multiple modules. The LDC-pyramid adaptive feature fusion module, combined with the SURF algorithm, comprehensively and stably extracts multi-scale features, enhancing adaptability to complex scenes. The SCIEAM module weights these features from channel and spatial dimensions, highlighting key pathogen features. The VSS block effectively captures local and global features, accurately fusing information from different regions and scales. The multimodal feature fusion and refinement module utilizes the SS2D mechanism and MLP deep feature optimization to mine potential connections between multimodal features. The introduced VSS block, based on the advantages of Mamba, reduces computational costs and improves inference speed, making the entire fusion process more efficient. The fused image processed by the multi-scale residual feature reconstruction module clearly presents pathogen information, significantly improving the level of livestock and poultry disease prevention and control.

The invention provides an intelligent dynamic monitoring method and system for livestock and poultry diseases, and the method comprises the steps: obtaining the historical behavior data of livestock and poultry, carrying out the clustering and numbering, constructing a feeding range preemption model and an activity range model through the historical feeding range data, obtaining the real-time feeding range data, carrying out the range preemption judgment, and generating a preemption event report. The method comprises the following steps: acquiring real-time activity range data, comparing the real-time activity range data with historical activity range data to carry out abnormal judgment, generating an activity range mutation report, generating a health risk assessment matrix through a preemption event report and the activity range mutation report, carrying out risk grade division on the health state of livestock and poultry through a risk grade library, and implementing a corresponding response strategy. The health risk assessment matrix is obtained through the feeding range and the activity range of the livestock and poultry, so that whether the livestock and poultry suffer from nutritional diseases and infectious diseases or not is judged, and the early recognition capacity and the coping capacity of the livestock and poultry diseases are improved through an intelligent detection method of multi-source data collection, behavior analysis and assessment.