59 results about "Poultry disease" patented technology

The invention relates to the technical field of livestock and poultry epidemic disease management, and particularly discloses an intelligent livestock and poultry epidemic disease early warning and partitioned prevention and control management system and method, and the method comprises the steps: laying an integrated intelligent sensor network in a livestock and poultry farm, and collecting environment parameters, feeding management data and animal health data in real time through a low-power-consumption Internet of Things technology; transmitting the collected multi-dimensional data to a cloud platform by utilizing an edge computing technology; based on multi-dimensional data, a deep learning and multi-source data fusion analysis technology is adopted, a dynamic disease risk prediction model is constructed, and generalization ability of an adversarial generative network optimization model in a complex environment is introduced; by arranging the integrated intelligent sensor network, environmental parameters, feeding management data and animal health data in the livestock and poultry farm are comprehensively collected in real time, the early monitoring capability of epidemic diseases is remarkably improved, potential epidemic disease risks are found in time, and precious time is won for subsequent prevention and control work.

The invention relates to the technical field of knowledge graph construction, in particular to a poultry disease knowledge graph construction method, which comprises the following steps of S1, defining a top layer ontology framework; s2, performing term merging on the multi-source poultry disease data to generate a standardized term set; s3, constructing an environment-pathogen-host chain type triple based on the ontology framework and the standardized term set; s4, performing space-time alignment and conflict resolution on the chain triad in the S3, and outputting a fusion knowledge base; s5, extracting a triple change sequence of continuous time slices based on the fusion knowledge base in the S4, and generating a dynamic propagation chain; and S6, executing logic verification and backtracking verification on the dynamic propagation chain in the S5, and outputting a finally constructed poultry disease knowledge graph. According to the method, through multi-source data standardization, dynamic propagation modeling and logic verification fusion, structured, dynamic and credible expression of the poultry disease propagation path is realized, and a complete and reliable knowledge support is provided for accurate prevention and control.

The invention discloses a management task generation method for livestock and poultry health intelligent monitoring based on multi-modal sensing, and relates to the technical field of agricultural informationization, and the method comprises the steps: building a disease causal network of a livestock and poultry breeding type, and generating a known disease multi-label time-varying vector of each stage of the livestock and poultry breeding type; establishing a multi-modal behavior encoder of the livestock and poultry breeding type, extracting each modal behavior vector of the real-time target livestock and poultry breeding type and the known disease multi-label time-varying vector of each stage of the livestock and poultry breeding type for correlation matching screening, and obtaining a concerned known disease set of the real-time livestock and poultry breeding type; and analyzing the lethal risk probability of each concerned known disease of the bred livestock and poultry types in unit time, screening the concerned known disease lethal risk probability and a known disease treatment task library, establishing a livestock and poultry disease treatment resource constraint function, and generating a priority sequence of the concerned known disease initial treatment tasks of the real-time bred livestock and poultry types. The death rate and economic loss are reduced, and the intelligent management level of the farm is remarkably improved.

The invention discloses a method for rapidly obtaining chicken flocks capable of simultaneously resisting five kinds of subgroup avian leukosis and application, and belongs to the technical field of poultry disease-resistant breeding. The method comprises the following steps: firstly, obtaining primordial germ cells of a donor chicken containing an anti-avian leukosis subgroup genotype I and an anti-avian leukosis subgroup genotype II through in-vitro separation and cultivation, then injecting the primordial germ cells of the chicken into chick embryo blood vessels of a receptor chicken with wild type tvas / s and tvbs / s genotypes, performing incubation and cultivation, performing genetic typing, and finally obtaining the chicken with the anti-avian leukosis subgroup genotype I and the anti-avian leukosis subgroup genotype II. And finally obtaining chicken flocks capable of simultaneously resisting five kinds of subgroup avian leukosis. The method optimizes a chicken primordial reproduction in-vitro efficient culture system, can quickly obtain different chicken flocks capable of simultaneously resisting five kinds of avian leukosis subgroups, can be applied to utilization and preservation of resistance germplasm resources of excellent chicken breeds in China, can be applied to breeding of disease-resistant chickens of different breeds, greatly shortens the period of traditional poultry anti-avian leukosis breeding, and has a wide application prospect. The novel technology has good application value and popularization prospect.

The invention relates to the technical field of image fusion, and discloses a lightweight and efficient multi-modal data fusion method. According to the method, an improved MobileNetV3-Small network is adopted as a basic feature extractor, parallel branch design in a training stage is optimized through a structure re-parameterization technology, and the parallel branch design is combined into single convolution in a reasoning stage, so that the calculation cost is remarkably reduced; resNet50 is introduced as a teacher model, a soft label is generated through temperature scaling, a student model is guided in combination with KL divergence loss and cosine feature alignment loss, and high-precision feature extraction of a lightweight network is realized; a dynamic weight fusion mechanism is designed, microscopic fluorescence and microfluidic fluorescence image features are fused, and multi-modal information contribution is balanced; aiming at the characteristics of different modal images, preprocessing methods such as guided filtering, CLAHE contrast enhancement, time sequence difference artifact removal and the like are respectively adopted, and effective signals are enhanced. According to the algorithm, the pathogen detection precision is ensured, the model parameter quantity is reduced, and an efficient solution capable of being deployed in an embedded mode is provided for livestock and poultry epidemic disease screening.

The invention relates to the technical field of image processing, and discloses a multi-modal image fusion method based on Mama and an attention mechanism. According to the method, through cooperative work of multiple modules, the multi-modal image fusion effect is greatly improved, the LDC-pyramid adaptive feature fusion module is combined with the SURF algorithm, multi-scale features are comprehensively and stably extracted, the adaptability to complex scenes is enhanced, the SCIEAM module weighs the features from channel and space dimensions, key features of pathogens are highlighted, and the robustness of the multi-modal image fusion is improved. The VSS block effectively captures local and global features and accurately fuses information of different regions and scales, the multi-modal feature fusion and refining module deeply optimizes features by using an SS2D mechanism and an MLP and excavates potential relations among multi-modal features, the introduced VSS block is based on the advantage of Mama, the calculation cost is reduced, the reasoning speed is increased, the whole fusion process is more efficient, and the fusion efficiency is improved. A fusion image obtained through processing of the multi-scale residual error feature reconstruction module can clearly present pathogen information, and the livestock and poultry disease prevention and control level is greatly improved.

The invention discloses a PCR primer group for detecting and distinguishing avian reoviruses and application of the PCR primer group, and belongs to the technical field of molecular biology. The invention provides a PCR primer for detecting and / or distinguishing the avian reovirus, the primer group has good specificity on the avian reovirus and has no cross reaction with other major poultry diseases, a sensitivity test result shows that the sensitivity is about 10 times higher than that of a conventional PCR method, other common poultry viruses have no specific amplification, and the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result shows that the detection result is about 10 times higher than that of the conventional PCR method. No cross reaction is found, intra-batch and inter-batch variation coefficients are both smaller than 2%, and the detection method has the advantages of being high in sensitivity, high in specificity, good in repeatability and the like.

The application discloses a sweet wormwood extract, a preparation method and application thereof and a sweet wormwood extract veterinary drug, and the method comprises the following steps: S1, heating and extracting sweet wormwood byproducts with an extraction solvent, filtering, discarding the filtrate, and obtaining an intermediate product filter cake; S2, concentrating the intermediate product filter cake to a constant weight; S3, heating and extracting the constant weight obtained in the step S2 with deionized water, filtering, and taking the filtrate; S4, concentrating the filtrate obtained in the step S3 to a constant weight, and obtaining the sweet wormwood extract. The application provides a sweet wormwood extract and a preparation method thereof, and provides a veterinary drug based on the sweet wormwood extract; the veterinary drug obtained by the application can clear heat, resolve summer-heat, and relieve deficiency heat, and can be used for treating various livestock and poultry diseases; the preparation method is simple, easy to operate, raw materials are easy to obtain, production cost is low, and the application has a good market application prospect.

The invention relates to the technical field of microorganisms. The invention provides a Klebsiella oxytoca bacteriophage RDP-EC-23031 strain, and the Klebsiella oxytoca bacteriophage RDP-EC-23031 strain is preserved in the China General Microbiological Culture Collection Center on April 22, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.45963. The Klebsiella oxytoca bacteriophage RDP-EC-23031 strain has the advantages that the Klebsiella oxytoca bacteriophage RDP-EC-23031 strain can be used for preparing the Klebsiella oxytoca bacteriophage; the invention also provides a phage composition. The invention also provides application of the klebsiella oxytoca phage RDP-EC-23031 or the phage composition in preparation of a product for preventing and / or treating livestock and poultry diseases caused by klebsiella oxytoca and application of the klebsiella oxytoca phage RDP-EC-23031 or the phage composition in preparation of a product for killing and / or inhibiting klebsiella oxytoca. The invention also provides a bacteriophage pharmaceutical preparation, a drinking water additive, a feed additive, a disinfectant or a cleaning agent. The Klebsiella oxytoca bacteriophage RDP-EC-23031 provided by the invention is used for replacing antibiotics to be applied to prevention and treatment of livestock and poultry breeding, and solves the problems that diseases of livestock and poultry animals are acute and cannot be treated in time, and drug use is invalid due to drug resistance of pathogenic bacteria.

The invention belongs to the technical field of livestock and poultry epidemic disease detection, and provides a portable blood sample collection device for livestock and poultry epidemic disease detection, which comprises a box body, one side of the box body is rotatably provided with a box door, the inner walls of the two sides of the box body are both provided with a plurality of groups of transverse plates, and adjusting assemblies are arranged between the transverse plates and the inner walls of the box body. A mounting plate is arranged between every two groups of transverse plates, guide rails are arranged on the sides, close to the mounting plates, of the transverse plates, guide blocks slidably connected to the guide rails are fixedly connected to the two sides of the mounting plates, a plurality of groups of placing grooves for placing test tubes are formed in the tops of the mounting plates in a penetrating manner, and clamping assemblies are arranged in the placing grooves; moving assemblies are arranged at four corners of the bottom of the box body; according to the device, test tubes with different lengths and diameters can be clamped and fixed through the adjusting assembly and the clamping assembly, the application range is effectively widened, the device can be moved more conveniently, and meanwhile, the device can be rapidly fixed after being moved.

The present invention discloses a drinking trough cleaning device and a poultry drinking water system for preventing poultry diseases, which relates to the technical field of poultry breeding. It includes a fixed frame, a support seat is fixedly connected to the top of the fixed frame, a water storage trough is inserted into the inner side wall of the support seat, a first water delivery pipe is inserted into the top of the support seat, and one end of the first water delivery pipe is fixedly connected to an adapter pipe. In the present invention, the driving component drives the conical joint to rotate and move inside the water storage trough, so as to use the arc-shaped cleaning piece to clean the inner wall of the water storage trough. It should be noted that while the water storage cylinder is moving, the cleaning plate at its top can simultaneously clean the drinking head, and under the action of the driving component, the time required for cleaning is shortened, saving the time and energy of the staff. At the same time, corresponding cleaning actions are made for the water storage trough and the drinking head, further improving the cleaning effect, guaranteeing the health of poultry, and bringing a better application prospect.

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The utility model discloses an anti-splashing device for poultry drinking water, and relates to the technical field of poultry drinking water. The utility model comprises: a base plate; the support is fixedly installed at the top of the bottom plate, a movable plate is installed on the support in a sliding mode, a splash-proof cover is fixedly installed at the bottom of the movable plate, and a water tank is fixedly installed between the two sides of the inner wall of the splash-proof cover. Through cooperation of the support, the movable plate, the splash-proof cover, the water tank, the adjusting assembly and the movable assembly, the normal drinking water requirement of poultry is met, meanwhile, the splashing phenomenon in the drinking water process is effectively prevented, waste of water resources is reduced, the humidity of the breeding environment is reduced, breeding of harmful microorganisms such as bacteria and molds is reduced, and the breeding quality is improved. The poultry disease probability is reduced, healthy growth of poultry is guaranteed, meanwhile, corrosion to breeding equipment is reduced, the service life of the equipment is prolonged, and breeding benefits are improved.

1. The name of this design product: Intelligent poultry disease diagnostic device. 2. Purpose of this design product: used for rapid and accurate diagnosis of poultry diseases. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The application belongs to the field of biology, and discloses a method for high-throughput screening of viral CTL epitopes based on immunopeptidomics, which comprises the following steps: transfecting mammalian cells with eukaryotic expression plasmids having MHC I molecules in series with the alpha chain and the beta2m chain to establish a mammalian cell line expressing animal MHC I molecules; using a virus to infect the mammalian cell line expressing MHC I molecules to prepare MHC I-peptide complexes; and obtaining antigen peptides existing in the MHC I-peptide complexes. The method takes chicken MHC I allele BF2*1901 molecules as the research object, takes H9N2 subtype avian influenza virus as the model virus, comprehensively characterizes chicken MHC I restricted H9N2 antigen peptide groups, and identifies immunodominant CTL epitope immunity, and is suitable for CTL epitope screening of different chicken MHC I molecules and different subtypes of avian influenza viruses, provides a fast, efficient and economical technical means for studying the specific binding of chicken MHC I molecules and antigen peptides, provides a favorable reference for T cell epitope screening of major animal pathogens, and provides a scientific basis for poultry disease-resistant breeding, development of new vaccines and immune evaluation strategies. Meanwhile, the application also discloses restricted epitope peptides, nucleic acid molecules and applications.

The invention relates to the technical field of microorganisms. The invention provides a strain of Gamaleyavius phage RKP-VP21052, the strain of Gamaleyavius phage RKP-VP21052 is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on September 27, 2024, and the preservation number of the Gamaleyavius phage RKP-VP21052 is CGMCC No.46192. The invention also provides a preparation method of the Gamaleyavius phage. The invention also provides a phage composition. The invention also provides an application of the Gamaleyavius bacteriophage RKP-VP21052 or the bacteriophage composition in the preparation of a product for preventing and / or treating livestock and poultry diseases caused by escherichia coli. The invention further provides the application of the Gamaleyavius bacteriophage RKP-VP21052 or the bacteriophage composition. The invention also provides a bacteriophage pharmaceutical preparation, wherein the effective components of the bacteriophage pharmaceutical preparation comprise the Gamaleyavius bacteriophage RKP-VP21052 or the bacteriophage composition disclosed by the invention. The invention also provides a drinking water additive, a feed additive, a disinfectant, a cleaning agent and the like. The Gamaleyavius bacteriophage RKP-VP21052 provided by the invention has a very good advantage in the aspect of reducing the concentration of escherichia coli, does not cause damage to probiotics, and is relatively high in titer and good in prevention and treatment effect.

The invention relates to the technical field of poultry disease detection, in particular to a hemagglutination test detection method for avian influenza and Newcastle disease. The invention does not perform hemagglutination tests for avian influenza and Newcastle disease, but sets 9log2 and 10log2 as reference hemagglutination titers for hemagglutination tests for avian influenza and Newcastle disease, respectively, prepares corresponding 4HAU antigens according to "Avian influenza and Newcastle disease 4HAU antigen preparation table", directly performs regression test, observes the number of erythrocyte agglutination holes, and calculates accurate avian influenza hemagglutination titer or Newcastle disease hemagglutination titer according to the observed result of the regression test according to the avian influenza hemagglutination titer formula or the Newcastle disease hemagglutination titer formula, without repeated test verification, thereby greatly improving the diagnostic efficiency of the avian influenza and Newcastle disease hemagglutination tests.

The invention relates to a poultry disease detection system based on a knowledge graph. The poultry disease detection system mainly comprises a data acquisition module and a disease recognition module, wherein the data acquisition module is used for reading poultry dissection pictures and corresponding text descriptions; and the disease recognition module is used for recognizing symptom features by adopting a Clip model according to the poultry dissection pictures and the corresponding text description, and acquiring corresponding disease names, treatment methods and environmental influence factors based on the symptom features and a knowledge graph. According to the application, through cooperative operation of the image recognition model and the knowledge graph, rapid, accurate and comprehensive detection of the poultry respiratory diseases can be realized.

The invention belongs to the technical field of poultry disease detection, and particularly relates to a poultry immunosuppressive disease joint detection method based on multiple fluorescent quantitative PCR (Polymerase Chain Reaction). The method sequentially comprises the following steps: determining a detection target, designing a specific primer and a probe, preparing a positive standard substance, pre-treating a sample, extracting nucleic acid, purifying a nucleic acid sample, establishing a multiple fluorescent quantitative PCR reaction system, setting a multiple fluorescent quantitative PCR reaction program, amplifying, and analyzing and judging a result. On the basis, by designing a multiplex fluorescent quantitative PCR detection system and the specific primer probe, the kit has the advantages that synchronous detection of multiple poultry immunosuppressive pathogens is realized, multiple independent detection on the same sample is not needed, the operation process is simplified, the detection cost is reduced, and the waste of the sample is reduced; the problems that a traditional single pathogen detection method is low in efficiency and high in cost are solved.

The utility model relates to a vaccine injection device for poultry disease prevention and control, which belongs to the field of poultry disease prevention and control and comprises a bottom plate, and an infusion structure for infusion is arranged on the upper surface of the bottom plate. The liquid conveying structure comprises a sound insulation cover fixedly connected to the upper surface of the bottom plate, a vibration buffering plate fixedly connected to the inner bottom wall of the sound insulation cover, a constant delivery pump fixedly connected to the upper surface of the vibration buffering plate, a liquid pumping pipe fixedly connected to the liquid pumping end of the constant delivery pump, a vaccine tank fixedly connected to the other end of the liquid pumping pipe and a heating assembly arranged on the outer side of the vaccine tank; the liquid outlet pipe is fixedly connected to the liquid outlet end of the quantitative pump, and an injection assembly used for injecting vaccines is arranged on the upper surface of the bottom plate. According to the poultry disease prevention and control vaccine injection device, in the process of performing vaccine injection on poultry, an injector does not need to be manually held by hand, so that injection point deviation caused by hand trembling during manual injection is avoided, the injection stability is improved, and accurate alignment and stable fixation of poultry injection parts are realized.

The invention discloses a livestock and poultry disease root cause diagnosis and intervention recommendation method, relates to the technical field of livestock and poultry disease diagnosis, and solves the problem that root cause diagnosis is difficult to realize in the prior art. According to the embodiment of the invention, the disease occurrence of the individual livestock and poultry is regarded as the result of the multi-agent dynamic game, and the root cause back-stepping is carried out by utilizing the inverse reinforcement learning, so that the root cause diagnosis result and the intervention recommendation suggestion are obtained, decision support for direct action is provided, and the management intelligence degree is greatly improved. Meanwhile, the game reasoning model comprehensively considers the effects of environment, group behaviors and biological pathogens, avoids the one-sidedness of single factor diagnosis, and is more scientific and reliable in conclusion.

The utility model discloses a livestock and poultry epidemic disease prevention and control device, which relates to the technical field of livestock and poultry epidemic disease prevention and control and comprises a movable plate trailer and a disinfectant storage box connected to the top of the movable plate trailer. The shunting spraying pipe is rotationally arranged on the movable plate trailer; the stirring rod is rotationally arranged in the disinfectant storage box; the servo motor is connected to the disinfectant storage box; the stirring rod drives the abutting cam to rotate in the rotating process, the abutting cam can abut against the sliding plate and enable the sliding plate to drive the transmission rack to horizontally move after rotating to a certain position, the driven gear can be driven to rotate in the moving process of the transmission rack, and therefore the stirring rod can be driven to rotate. According to the utility model, the spraying angle can be adjusted while the disinfectant is stirred and sprayed through the arranged spraying adjusting assembly, so that the spraying flexibility is improved, and the spraying angle of the disinfectant can be adjusted while the disinfectant is stirred and sprayed through the arranged spraying adjusting assembly.

The utility model discloses a medicine dispensing device for animal husbandry veterinarians, which relates to the technical field of medicine dispensing devices for veterinarians and comprises a mounting bottom plate, and four L-shaped support plates are fixedly connected to the upper end of the mounting bottom plate. According to the crushing device, the two crushing rollers can be driven to rotate relatively through mutual matching of the rotating rod I, the belt pulley I, the gear, the rotating shaft I and the double-groove belt pulley, so that the crushing process of large-particle medicines entering the shell can be achieved; the crushed medicine can be quickly mixed with the feed under the action of a rotating rod I, a bevel gear I, a bevel gear II, a rotating rod II and a stirring rod, and at the moment, the medicine dispensing process can be completed, so that the poultry can eat the medicine in the feeding process, and the effects of treating and preventing the poultry diseases are achieved; and in the crushing process of the large-particle medicine, a rotating shaft II can be driven to rotate under the action of an arranged double-groove belt wheel and a belt wheel II.

The invention discloses a preparation method and application of an oral chicken infectious anemia vaccine. The preparation method comprises the following steps: providing an eimeria vector; genes of VP1 protein and VP2 protein for coding the chicken infectious anemia virus are introduced into the eimeria vector; after oral immunization, the recombinant eimeria can effectively reduce the clinical symptoms and virus load of chicken lean transmission, saves the manpower and material resources of vaccine immunization, is suitable for prevention and control of poultry epidemic diseases in large-scale breeding industry, and has a wide application prospect.

The invention relates to the technical field of microorganisms. The invention provides a lytic enterobacter cancerogenic bacteriophage RDP-QEC-24001, the lytic enterobacter cancerogenic bacteriophage RDP-QEC-24001 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on September 23, 2024, and the preservation number of the lytic enterobacter cancerogenic bacteriophage RDP-QEC-24001 is CGMCC (China General Microbiological Culture Collection Center) No.46191. The invention further provides a preparation method of the lytic enterobacter cancerogenic bacteriophage RDP-QEC-24001. Based on the same inventive concept, the invention also provides a bacteriophage composition. The invention also provides application of the bacteriophage RDP-QEC-24001 for splitting the carcinogenic enterobacter or the bacteriophage composition in preparation of products for preventing and / or treating livestock and poultry diseases caused by the carcinogenic enterobacter and application of the bacteriophage composition in preparation of products for killing and / or inhibiting the carcinogenic enterobacter. The invention also provides a bacteriophage pharmaceutical preparation, a drinking water additive, a feed additive, a disinfectant or a cleaning agent. The split carcinogenic enterobacteriophage RDP-QEC-24001 provided by the invention is high in titer, good in treatment effect and high in splitting rate.

The present invention provides a method and device for identifying the type of disease in livestock and poultry disease images, which relates to the technical field of image data processing. The method includes: obtaining livestock and poultry images to be processed, and calling a livestock and poultry image attribute extractor based on an inverse mapping generative adversarial network to extract appearance latent variable features; generating appearance latent variable features through a healthy livestock and poultry image generator based on a generative adversarial network to obtain healthy livestock and poultry images; determining a corrected difference map between the healthy livestock and poultry image and the livestock and poultry image to be processed, and calling an image classifier to classify the corrected difference map; when the classification result of the corrected difference map is a lesion area image, locating the type of disease lesion area of ​​the livestock and poultry image to be processed by using the corrected difference map. Through this application, the technical problem that the recognition accuracy and lesion area positioning accuracy still need to be improved in the application of livestock and poultry disease recognition in the prior art is solved.

This invention discloses a chicken infectious bronchitis virus (IBBV) nanoparticle, its preparation method, and its application, belonging to the field of poultry vaccine technology. The nanoparticles are formed by the self-assembly of an SRBD-SpyTag fusion protein and a Ferritin-SpyCatcher fusion protein via a SpyTag / SpyCatcher covalent linkage system. The SRBD-SpyTag fusion protein is formed by fusing a SpyTag sequence to the N-terminus or C-terminus of the S1 protein receptor-binding domain of IBV. The nanovaccine prepared from these nanoparticles exhibits high safety, rapid antigen module replacement, and is suitable for preventing IBV infection. The technical solution of this invention has advantages such as structural stability, strong immunogenicity, and rapid iteration, providing a novel vaccine solution for poultry disease control.

The invention relates to the technical field of poultry breeding, in particular to a method for judging poultry salmonella resistance based on resistance indexes. The invention provides a method for judging the resistance of avian salmonella based on resistance indexes. The resistance indexes comprise the intestinal mucosa layer thickness, the intestinal gland depth, the number of goblet cells in unit length, the ratio of heterophilic granulocytes to lymphocytes and the IFN-gamma concentration. At present, an effective salmonella disease resistance selection index lacks in the poultry breeding field, which is a key point of poultry disease resistance breeding. The salmonella resistance index provided by the invention not only can improve the efficiency and accuracy of disease resistance evaluation, but also provides important theoretical support for salmonella infection resistant breeding. The method fills the blank of poultry salmonella disease resistance selection indexes, and can greatly promote the poultry disease resistance breeding process.

The invention belongs to the technical field of feeding troughs, and particularly relates to an outdoor poultry feeding trough with an anti-pollution effect, which comprises a trough body, supporting legs are mounted at the bottom end of the trough body, a mounting frame is mounted at the top end of the trough body, an electric rod is connected to the top end of the mounting frame, and the electric rod is connected to the bottom end of the trough body. A mounting plate is arranged in the mounting frame, the top end of the mounting plate is connected with a box body, the innovation of the invention lies in that a mechanism is designed, and clean water and a disinfectant in the box body are utilized to effectively clean and disinfect the crib body. According to the mechanism, feed residues are cleaned through cooperation of the bottom plate and the cleaning agent, then the residues are cleaned through cooperation of the cleaning brush, comprehensive cleaning and disinfection are ensured, pollution to the feeding trough body is remarkably reduced, sanitation and safety of the feeding environment are guaranteed, the poultry disease risk is reduced, poultry health and production performance are improved, meanwhile, the cleaning process is simplified, and the labor intensity of feeders is relieved; and economic benefits are brought.

The invention discloses a method for producing a functional clostridium butyricum culture by using a clostridium butyricum centrifugate. The method comprises the following steps: (1) adding a protein raw material and a compound enzyme into the clostridium butyricum centrifugate, and reacting at 40-60 DEG C for 1-5 hours to finish a primary enzymolysis process; (2) compound strains are inoculated into the preliminary enzymolysis product, meanwhile, a carbon source is supplemented, a reaction is conducted for 6-18 h at the temperature of 25-40 DEG C, a secondary fermentation process is completed, an enzymolysis reaction is conducted in the process, the compound strains are composed of multiple lactic acid bacteria and saccharomycetes, and the lactic acid bacteria comprise lactobacillus casei, lactobacillus plantarum, lactobacillus acidophilus and enterococcus faecium; the saccharomycetes are kluyveromyces marxianus; and (3) carrying out spray drying on the fermented product to obtain the functional clostridium butyricum culture. The beneficial metabolites of the clostridium butyricum, the lactic acid bacteria and the saccharomycetes in the product not only have a very strong food calling effect and can promote digestion and absorption of feed, but also can effectively improve the immune level of livestock and poultry and inhibit livestock and poultry diseases.