19 results about "Allantoic fluid" patented technology

The invention discloses a tetravalent influenza virus subunit vaccine and a preparation method thereof, and relates to the technical field of biology, each dose of the tetravalent influenza virus subunit vaccine comprises two influenza A viruses and two influenza B viruses, the tetravalent influenza virus subunit vaccine is prepared by virus inoculation, virus multiplication culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, splitting and ultracentrifugation purification, mixing, filtration sterilization, split charging and packaging. Inactivation comprises the following steps: adding a cross-linked carboxymethyl glucan solution into the monovalent virus harvesting liquid, adding formaldehyde, and inactivating at 5 DEG C for 45 hours; then genipin is mixed into the virus liquid, the stirring speed is 200 rpm, the stirring time is 8 h under the conditions that the pH is 7.2 and the temperature is 25 DEG C, residues of free methanol can be further reduced, migration of small molecules is inhibited, and the long-term storage stability is guaranteed.

The invention discloses a method for positioning and detecting key parts of hatching eggs. The method comprises the following steps: acquiring transmission images in hatching eggs of different days through a hatching egg image acquisition device, and training and establishing a hatching egg physiological state judgment model and a hatching egg key part segmentation model; the discrimination model outputs a transmission image of the fertilized egg to be positioned and detected, the key part is identified after the transmission image is processed by the segmentation model, then the optimal coordinate point extracted by the allantoic fluid is positioned by adopting an image processing method, and the allantoic fluid is extracted to realize the detection of the hatching egg. According to the method, a dual model of hatching egg development evaluation and key part identification is established, interference of human subjective factors is weakened, and detection and classification errors are reduced; the allantoic fluid coordinate positioning method reduces the risk of puncturing and damaging the embryo and improves the allantoic fluid extraction success rate; according to the invention, rapid and accurate hatching egg information evaluation and male and female chick embryo difference characteristic detection can be provided according to hatching application requirements.

The present invention discloses a method for locating and detecting key parts of hatching eggs. The method includes: obtaining transmission images inside hatching eggs of different days through a hatching egg image acquisition device, training and establishing a discriminant model for the physiological state of hatching eggs and a segmentation model for key parts of hatching eggs; the discriminant model outputs the transmission images of fertilized eggs to be located and detected, and after processing with the segmentation model, the key parts are identified, and then an image processing method is used to locate the optimal coordinate points for allantoic fluid extraction, and then the allantoic fluid is extracted to realize the detection of hatching eggs. The method of the present invention establishes a dual model for hatching egg development evaluation and key part recognition, weakens the interference of human subjective factors, and also reduces detection and classification errors; the allantoic fluid coordinate positioning method reduces the risk of puncturing and damaging embryos and improves the success rate of allantoic fluid extraction; the present invention can provide rapid and accurate hatching egg information evaluation and detection of differential characteristics between male and female chicken embryos according to the needs of hatching applications.

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The utility model relates to the technical field of hatching devices, and discloses a goose egg incubator which comprises an incubator shell, a cavity is formed in the bottom of the incubator shell, a ventilation cavity is formed in the inner cavity of the incubator shell, a fan is fixedly installed at the bottom end of the inner cavity of the ventilation cavity, and an air outlet is formed in the inner cavity of the incubator shell. A cylindrical water absorption sponge is placed at the bottom end of an inner cavity of the water storage tank, a water absorption sponge block is fixedly connected to the top end of the cylindrical water absorption sponge, a partition plate is fixedly connected to the side wall of the inner cavity of the incubator shell, ventilation holes are formed in the side face of the partition plate, and a ventilation opening is formed in the top end of the incubator shell. The constant-temperature incubator has the advantages that the constant-temperature incubator is provided with the water-absorbing sponge block, the fan and the vent holes, accordingly, evaporation of water in eggs can be reduced by the aid of high humidity at the early hatching stages, the water required by embryos can be kept, and the constant-temperature incubator is favorable for forming amniotic fluid and allantoic fluid of the embryos.

The utility model belongs to the technical field of biological pharmacy, and particularly relates to a chick embryo allantoic fluid collecting device which comprises a syringe needle, a needle stem and a pintle. The anti-blocking filtering piece comprises a sleeve, a housing and a connecting piece, the front end of the needle stem is covered with the housing, the risk that yolk is damaged in the collecting process can be reduced by means of the characteristics that the outer surface of the housing is smooth and free of edges and corners, and the egg yolk collecting device is convenient, rapid and high in yield and provides convenient conditions for follow-up production; a plurality of liquid inlet holes are formed outside the housing, so that liquid inlet paths can be dispersed, and the blocking risk caused by a single channel is reduced; the needle stem is sleeved with the sleeve, stable support is provided for the housing, and the stability of the collecting process is guaranteed; and finally, the connecting piece is arranged to be detachably connected with the pintle, so that assembling and disassembling can be facilitated, and cleaning and replacement are facilitated.

The invention relates to the technical field of biological medicine and vaccine preparation, and discloses a preparation method of a tetravalent influenza virus split vaccine, and the preparation method comprises the following steps: inoculating four influenza viruses into a chick embryo, culturing, harvesting allantoic fluid, and concentrating; centrifugally purifying through a sucrose density zone, and adding a cracking agent for cracking; removing the cracking agent from the cracking solution through chromatographic column chromatography, and then adding an inactivating agent for inactivating and removing the inactivating agent; according to the method disclosed by the invention, a process route of firstly splitting purification and then inactivation in combination with dual purification of centrifugation and chromatography is adopted, so that the problem of impurity cross-linking caused by an inactivator in a traditional process is effectively avoided, and residues of ovalbumin and splitting agents are deeply removed; and the natural conformation of the virus antigen is retained to the greatest extent. The obtained vaccine is extremely low in impurity content, has excellent safety and high immunogenicity, and can effectively expand the immune protection range and enhance the population immune barrier.

The present application discloses a quadrivalent influenza virus subunit vaccine and a preparation method thereof, which relate to the field of biotechnology. Each dose contains two influenza A viruses and two influenza B viruses. The quadrivalent influenza virus subunit vaccine is prepared by virus inoculation, virus proliferation and culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, lysis and ultracentrifugation purification, mixing, filtration sterilization, subpackaging and packaging; inactivation is performed by adding a cross-linked carboxymethyl dextran solution to the monovalent virus harvest liquid, and then adding formaldehyde to inactivate at 5°C for 45 hours; then, genipin is mixed into the virus liquid, stirred at a speed of 200 rpm, and stirred for 8 hours at pH 7.2 and 25°C, which can further reduce the residual free methanol, inhibit the migration of small molecules, and ensure long-term storage stability.

The invention discloses a chick embryo allantoic fluid collecting device for trial production of poultry multi-combined inactivated vaccines, relates to the technical field of vaccine preparation, and solves the technical problems that before allantoic fluid is extracted, the pre-preparation procedure is complicated, chick embryos are inconvenient to treat, and meanwhile, the allantoic fluid is easy to pollute during shelling. A conveying mechanism is installed on the front surface of the supporting frame, a shell breaking mechanism is arranged on one side of the top of the supporting frame, a shelling mechanism is arranged at the bottom of the shell breaking mechanism, and a discharging mechanism is arranged on one side of the front end of the conveying mechanism. Through mutual cooperation of the mechanisms, when the allantoic fluid of the chick embryo needs to be extracted, the chick embryo is firstly subjected to shell breaking treatment, then the eggshell at the eggshell position of the chick embryo subjected to shell breaking treatment is stripped, and the treated chick embryo is taken out from the chain plate conveying belt, so that subsequent collection of the allantoic fluid of the chick embryo is facilitated; and the pre-treatment speed of the chick embryo before the allantoic fluid of the chick embryo is extracted is accelerated.

The utility model discloses an inoculating and harvesting all-in-one machine, which comprises a sterilizing device used for sterilizing the upper surface of an embryo egg; the inoculation equipment comprises an egg jacking module, an inoculation module and a disinfection module, the inoculation module comprises an inoculation needle, and the disinfection module is used for disinfecting the inoculation needle; the harvesting equipment comprises a shell cutting module, a harvesting module and a detection module, the shell cutting module is used for cutting off the upper eggshell of the embryo egg, the detection module is used for detecting whether the embryo egg is qualified or not, the harvesting module comprises a plurality of harvesting heads, and the detection module is used for detecting the quality of the harvested liquid; and the caching equipment is used for caching the egg trays. The embryo egg processing device has the beneficial effects that the processes of embryo egg transmission, embryo egg disinfection, egg jacking, punching, virus inoculation, embryo egg shell cutting, embryo egg screening and allantoic fluid harvesting are realized, the processing efficiency is high, the pollution is reduced, and the production quality is improved.

The present invention discloses a method for detecting the sex of hatching eggs in the early stage. The method includes: extracting allantoic fluid from hatching eggs in the early stage through an extraction device; obtaining the allantoic fluid metabolite characteristics after metabolomics analysis of the allantoic fluid, and performing bioinformatics analysis combined with in vitro cell culture to confirm sex-differentiated metabolites; further concentrating and fractionating the allantoic fluid to obtain low-abundance allantoic fluid containing sex-differentiated metabolites; performing spectral comparison analysis on the allantoic fluid to be detected, thereby constructing a multi-machine learning allantoic fluid sex classification model; detecting the sex of hatching eggs in the early stage through the model to achieve rapid detection of the sex of eggs. The full-chain process of "early minimally invasive sampling - differential metabolite screening - low-abundance component enrichment - multi-spectral machine learning" designed by the present invention realizes breakthroughs in multiple dimensions such as time window, accuracy, and cost controllability for poultry egg sex detection.

An automatic vaccine harvesting device comprises a feeding device used for being in butt joint with an upstream device conveying egg tray; the shell cutting device comprises a conveying module and a shell cutting module and is used for cutting off an eggshell on the upper side of the embryo egg on the egg tray; the harvesting device comprises a harvesting module and a detection module and is used for harvesting allantoic fluid in the embryo eggs and detecting whether the allantoic fluid is qualified or not; the crushing equipment comprises a transfer mechanism, a turnover mechanism and a crushing mechanism, the turnover mechanism turns over the egg tray, and the embryo eggs fall into the crushing mechanism on the lower side to be crushed. The chick embryo harvesting machine has the advantages that chick embryo conveying, chick embryo disinfection, chick embryo shell cutting, broken shell discharging, chick embryo screening, allantoic fluid harvesting, waste embryo treatment and other operations are achieved, the harvesting efficiency is high, and the harvesting quality is guaranteed.

An isolated infectious bronchitis (IB) virus (IBV) QX1285 strain under accession number DPS RE RSCIC 41 in the IZSLER biobank. A vaccine comprising the isolated infectious bronchitis virus and a carrier. The virus may be inactivated. The virus may alternatively be live and attenuated. The vaccine may be formulated for an avian. The avian may be a chicken. The vaccine may be administrated via drinking water. Also claimed is an isolated infectious bronchitis virus having an S1 protein encoded by SEQ ID NO: 1 or a sequence having at least 96% sequence identity thereto, for use as a vaccine against infectious bronchitis. Also claimed is a method of vaccinating an avian against infectious bronchitis, comprising administering the vaccine. Also claimed is a process for preparing a live infectious bronchitis vaccine comprising passaging the virus to reduce pathogenicity, harvesting the attenuated virus, and processing the virus to produce a vaccine. The virus may be passaged at least 50 times. The virus may be passaged by culturing in allantoic fluid of chicken embryos.

The invention discloses an inoculating and harvesting all-in-one machine, which comprises a disinfection device for disinfecting the upper surface of an embryo egg; the inoculation equipment comprises an egg jacking module, an inoculation module and a disinfection module, the inoculation module comprises an inoculation needle, and the disinfection module is used for disinfecting the inoculation needle; the harvesting equipment comprises a shell cutting module, a harvesting module and a detection module, the shell cutting module is used for cutting off the upper eggshell of the embryo egg, the detection module is used for detecting whether the embryo egg is qualified or not, the harvesting module comprises a plurality of harvesting heads, and the detection module is used for detecting the quality of the harvested liquid; and the caching equipment is used for caching the egg trays. The device has the beneficial effects that the processes of embryo egg conveying, embryo egg disinfection, egg jacking, punching, virus inoculation, embryo egg shell cutting, embryo egg screening and allantoic fluid harvesting are achieved, the processing efficiency is high, pollution is reduced, and the production quality is improved.

The invention relates to the field of prevention and control of poultry viral diseases, in particular to application of herba epimedii extract in preparation of a medicine for preventing and / or treating infectious bronchitis virus infection. The herba epimedii extract is a herba epimedii ethanol extract. The biological safety and antiviral activity of the extract are evaluated by constructing a chick embryo infection model, and the epimedium extract does not generate obvious toxic influence on chick embryos; after infectious bronchitis virus infection, the survival rate of chick embryos can be increased. Furthermore, tissue immunofluorescence detection, western blot analysis and probe method fluorescent quantitative PCR detection results show that the extract can reduce the virus antigen expression level and virus nucleic acid copy number in chick embryo liver tissue and allantoic fluid, and therefore the effect of preventing or treating IBV infection is achieved. The herba epimedii extract is stable in raw material source, simple and convenient in preparation process and relatively low in production cost, and has a good popularization and application prospect.

The invention discloses a method for detecting the sex of hatching eggs at the early hatching stage. The method comprises the following steps: extracting allantoic fluid for hatching early hatching eggs through an extraction device; carrying out metabonomics analysis on allantoic fluid to obtain allantoic fluid metabolite characteristics, and carrying out bioanalysis in combination with in-vitro cell culture to confirm gender difference metabolites; carrying out concentration grading treatment on the allantoic fluid to obtain low-abundance allantoic fluid containing gender difference metabolites; performing spectral comparative analysis on the to-be-detected allantoic fluid so as to construct a multi-machine learning allantoic fluid gender classification model; the sex of the hatching eggs in the early hatching period is detected through the model, and rapid sex detection of the hatching eggs is achieved. According to the full-chain process of early-stage minimally invasive sampling, differential metabolite screening, low-abundance component enrichment and multispectral machine learning, breakthrough of egg gender detection in multiple dimensions of time window, accuracy, cost controllability and the like is achieved.

An automatic vaccine harvesting device comprises a feeding device used for being in butt joint with an upstream device conveying egg tray; the shell cutting device comprises a conveying module and a shell cutting module and is used for cutting off an eggshell on the upper side of the embryo egg on the egg tray; the harvesting device comprises a harvesting module and a detection module and is used for harvesting allantoic fluid in the embryo eggs and detecting whether the allantoic fluid is qualified or not; the crushing equipment comprises a transfer mechanism, a turnover mechanism and a crushing mechanism, the turnover mechanism turns over the egg tray, and the embryo eggs fall into the crushing mechanism on the lower side to be crushed. The chick embryo harvesting machine has the advantages that chick embryo conveying, chick embryo disinfection, chick embryo shell cutting, broken shell discharging, chick embryo screening, allantoic fluid harvesting, waste embryo processing and other operations are achieved, the harvesting efficiency is high, and the harvesting quality is guaranteed.

The application discloses an influenza virus subunit vaccine and a preparation method thereof, and relates to the field of biological medicines. Each dose contains H1N1, H3N2 and Victoria; the influenza virus subunit vaccine is prepared through virus inoculation, proliferation culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, lysis purification, mixing, sterilization filtration and subpackaging. The application significantly enhances the dendritic cell targeted uptake and rapid release of antigens through the design of mannose modified small liposomes and pH responsive channels; in combination with the precise controlled release of the graded lysis micelles, the antibody titer and immunological persistence are obviously improved, the single dose protection period is longer, and the defects of the traditional trivalent vaccine in the insufficient immunological intensity are effectively made up.

The invention provides chick embryo allantoic fluid harvesting equipment, which belongs to the technical field of vaccine production, and comprises an equipment shell, a fluid collection pool, a chick embryo tray, a chick embryo cutter, an allantoic fluid collection pipe and a disinfection spray head, the equipment shell is provided with a shell inner cavity with a bottom opening; the liquid collecting pool is arranged below the equipment shell and is over against the lower opening of the inner cavity of the shell; the chick embryo tray is arranged at the bottom of the inner cavity of the shell, and chick embryo balancing holes are formed in the chick embryo tray; the chick embryo cutter is arranged in the inner cavity of the shell and located above the chick embryo tray, and the chick embryo cutter can move in the preset horizontal direction; the allantoic fluid collecting tube is arranged at the top of the inner cavity of the shell; the disinfection nozzle is arranged at the top of the inner cavity of the shell, is connected to the top wall of the inner cavity of the shell in a liftable manner, and is also communicated with the disinfectant preparation pool. The problem of allantoic fluid pollution caused by the fact that cut open chick embryos are transferred between different stations can be avoided, the problem of poor disinfection can be avoided, and the disinfection reliability is guaranteed.