9 results about "Allantoic fluid" patented technology

The invention belongs to the technical field of poultry disease prevention and treatment, and discloses application of a rhizoma kaempferiae extract in preparation of a medicine for resisting avian infectious bronchitis virus. The rhizoma kaempferiae extract is prepared by taking rhizoma kaempferiae as a raw material and ethanol as an extraction solvent through extraction, filtration and concentration. Experimental results show that the rhizoma kaempferiae extract has no obvious toxicity to chick embryos within a certain dosage range; after the chicken embryo is infected with the infectious bronchitis virus, the survival rate of the chicken embryo can be improved by treating the chicken embryo with different doses of the rhizoma kaempferiae extract, and the rhizoma kaempferiae extract can reduce the virus antigen expression level and virus copy number in the liver tissue and allantoic fluid of the chicken embryo, so that the chicken embryo has prevention and treatment effects on infectious bronchitis virus infection. The rhizoma kaempferiae extract has the advantages of easily available raw materials, simple process and low cost, and is suitable for popularization and application.

The utility model belongs to the technical field of biological pharmacy, and particularly relates to a chick embryo allantoic fluid collecting device which comprises a syringe needle, a needle stem and a pintle. The anti-blocking filtering piece comprises a sleeve, a housing and a connecting piece, the front end of the needle stem is covered with the housing, the risk that yolk is damaged in the collecting process can be reduced by means of the characteristics that the outer surface of the housing is smooth and free of edges and corners, and the egg yolk collecting device is convenient, rapid and high in yield and provides convenient conditions for follow-up production; a plurality of liquid inlet holes are formed outside the housing, so that liquid inlet paths can be dispersed, and the blocking risk caused by a single channel is reduced; the needle stem is sleeved with the sleeve, stable support is provided for the housing, and the stability of the collecting process is guaranteed; and finally, the connecting piece is arranged to be detachably connected with the pintle, so that assembling and disassembling can be facilitated, and cleaning and replacement are facilitated.

The invention relates to the technical field of biological medicine and vaccine preparation, and discloses a preparation method of a tetravalent influenza virus split vaccine, and the preparation method comprises the following steps: inoculating four influenza viruses into a chick embryo, culturing, harvesting allantoic fluid, and concentrating; centrifugally purifying through a sucrose density zone, and adding a cracking agent for cracking; removing the cracking agent from the cracking solution through chromatographic column chromatography, and then adding an inactivating agent for inactivating and removing the inactivating agent; according to the method disclosed by the invention, a process route of firstly splitting purification and then inactivation in combination with dual purification of centrifugation and chromatography is adopted, so that the problem of impurity cross-linking caused by an inactivator in a traditional process is effectively avoided, and residues of ovalbumin and splitting agents are deeply removed; and the natural conformation of the virus antigen is retained to the greatest extent. The obtained vaccine is extremely low in impurity content, has excellent safety and high immunogenicity, and can effectively expand the immune protection range and enhance the population immune barrier.

The present application discloses a quadrivalent influenza virus subunit vaccine and a preparation method thereof, which relate to the field of biotechnology. Each dose contains two influenza A viruses and two influenza B viruses. The quadrivalent influenza virus subunit vaccine is prepared by virus inoculation, virus proliferation and culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, lysis and ultracentrifugation purification, mixing, filtration sterilization, subpackaging and packaging; inactivation is performed by adding a cross-linked carboxymethyl dextran solution to the monovalent virus harvest liquid, and then adding formaldehyde to inactivate at 5°C for 45 hours; then, genipin is mixed into the virus liquid, stirred at a speed of 200 rpm, and stirred for 8 hours at pH 7.2 and 25°C, which can further reduce the residual free methanol, inhibit the migration of small molecules, and ensure long-term storage stability.

An automatic vaccine harvesting device comprises a feeding device used for being in butt joint with an upstream device conveying egg tray; the shell cutting device comprises a conveying module and a shell cutting module and is used for cutting off an eggshell on the upper side of the embryo egg on the egg tray; the harvesting device comprises a harvesting module and a detection module and is used for harvesting allantoic fluid in the embryo eggs and detecting whether the allantoic fluid is qualified or not; the crushing equipment comprises a transfer mechanism, a turnover mechanism and a crushing mechanism, the turnover mechanism turns over the egg tray, and the embryo eggs fall into the crushing mechanism on the lower side to be crushed. The chick embryo harvesting machine has the advantages that chick embryo conveying, chick embryo disinfection, chick embryo shell cutting, broken shell discharging, chick embryo screening, allantoic fluid harvesting, waste embryo treatment and other operations are achieved, the harvesting efficiency is high, and the harvesting quality is guaranteed.

An isolated infectious bronchitis (IB) virus (IBV) QX1285 strain under accession number DPS RE RSCIC 41 in the IZSLER biobank. A vaccine comprising the isolated infectious bronchitis virus and a carrier. The virus may be inactivated. The virus may alternatively be live and attenuated. The vaccine may be formulated for an avian. The avian may be a chicken. The vaccine may be administrated via drinking water. Also claimed is an isolated infectious bronchitis virus having an S1 protein encoded by SEQ ID NO: 1 or a sequence having at least 96% sequence identity thereto, for use as a vaccine against infectious bronchitis. Also claimed is a method of vaccinating an avian against infectious bronchitis, comprising administering the vaccine. Also claimed is a process for preparing a live infectious bronchitis vaccine comprising passaging the virus to reduce pathogenicity, harvesting the attenuated virus, and processing the virus to produce a vaccine. The virus may be passaged at least 50 times. The virus may be passaged by culturing in allantoic fluid of chicken embryos.

The invention discloses an inoculating and harvesting all-in-one machine, which comprises a disinfection device for disinfecting the upper surface of an embryo egg; the inoculation equipment comprises an egg jacking module, an inoculation module and a disinfection module, the inoculation module comprises an inoculation needle, and the disinfection module is used for disinfecting the inoculation needle; the harvesting equipment comprises a shell cutting module, a harvesting module and a detection module, the shell cutting module is used for cutting off the upper eggshell of the embryo egg, the detection module is used for detecting whether the embryo egg is qualified or not, the harvesting module comprises a plurality of harvesting heads, and the detection module is used for detecting the quality of the harvested liquid; and the caching equipment is used for caching the egg trays. The device has the beneficial effects that the processes of embryo egg conveying, embryo egg disinfection, egg jacking, punching, virus inoculation, embryo egg shell cutting, embryo egg screening and allantoic fluid harvesting are achieved, the processing efficiency is high, pollution is reduced, and the production quality is improved.

The invention relates to the field of prevention and control of poultry viral diseases, in particular to application of herba epimedii extract in preparation of a medicine for preventing and / or treating infectious bronchitis virus infection. The herba epimedii extract is a herba epimedii ethanol extract. The biological safety and antiviral activity of the extract are evaluated by constructing a chick embryo infection model, and the epimedium extract does not generate obvious toxic influence on chick embryos; after infectious bronchitis virus infection, the survival rate of chick embryos can be increased. Furthermore, tissue immunofluorescence detection, western blot analysis and probe method fluorescent quantitative PCR detection results show that the extract can reduce the virus antigen expression level and virus nucleic acid copy number in chick embryo liver tissue and allantoic fluid, and therefore the effect of preventing or treating IBV infection is achieved. The herba epimedii extract is stable in raw material source, simple and convenient in preparation process and relatively low in production cost, and has a good popularization and application prospect.

The application discloses an influenza virus subunit vaccine and a preparation method thereof, and relates to the field of biological medicines. Each dose contains H1N1, H3N2 and Victoria; the influenza virus subunit vaccine is prepared through virus inoculation, proliferation culture, allantoic fluid harvesting, clarification, inactivation, ultrafiltration concentration, lysis purification, mixing, sterilization filtration and subpackaging. The application significantly enhances the dendritic cell targeted uptake and rapid release of antigens through the design of mannose modified small liposomes and pH responsive channels; in combination with the precise controlled release of the graded lysis micelles, the antibody titer and immunological persistence are obviously improved, the single dose protection period is longer, and the defects of the traditional trivalent vaccine in the insufficient immunological intensity are effectively made up.