Preparation method and application of immunization preparation for controlling novel duck reovirus

A technology of reovirus and immune preparations, which is applied in the field of preparation and application of immune preparations for the prevention and treatment of new duck reovirus disease, and achieves the effects of low cost, simple preparation and good safety

Inactive Publication Date: 2012-06-13
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI +1
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[0011] The present invention will be further described below in conjunction with specific examples. (but not a limitation of the invention).

[0012] The duck reovirus NDRV-JM85 strain is a new type of duck reovirus that was passed down and bred through biotechnology. Deposited in CCTCC, deposit date July 29, 2011, deposit number: CCTCC NO: V201127.

[0013] Use the above-mentioned duck reovirus NDRV-JM85 strain as seed virus, inoculate the allantoic cavity of Muscovy duck embryos, inoculate the allantoic cavity with 11-day-old Muscovy duck embryo proliferation virus, and harvest toxic allantoic fluid; or inoculate Muscovy duck embryo adult Fibroblast monolayer, harvest the cytotoxicity when the cytopathic effect reaches more than 75%; inactivate the above-mentioned virus with formaldehyde, mix it with oil adjuvant in an appropriate ratio (such as 1:1 ratio), and develop into an inactivated vaccine. The laying hens were immunized with the inactivated vaccine, 1.0ml / feather, ...

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Abstract

The invention discloses a novel duck reovirus strain NDRV-JM85 with a preservation number of CCTCC NO: V201127. The novel duck reovirus strain NDRV-JM85 is used as a virus seed to inoculate an allantoic cavity of a muscovy duck embryo and obtain a virus-containing allantoic fluid, or inoculate an MDEF cell to obtain cytotoxin when a CPE reaches 75%. The virus liquid is inactivated by formaldehyde and added with a white oil adjuvant, and the mixture is mixed thoroughly to from a safe and effective oil emulsion inactivated vaccine for novel duck reovirus. The vaccine is used to immunize a laying hen three times, with an immunization interval of 21 days and 1.0 ml for each hen at each time. Eggs laid 21-63 days after the three immunizations are gained, and extracted yolk and a proper amount of disinfected normal saline is prepared into a hyperimmune antibody preparation for the novel duck reovirus. A duckling without an NDRV maternal antibody is injected with the antibody preparation at 1-2 days old for 0.5ml / duckling, or a duckling with a maternal antibody is injected with the antibody preparation at 10 days old for 1.0 ml / duckling, so as to prevent and control the novel duck reovirus. The invention applies to large-scale production under a GMP condition.

Description

technical field [0001] The invention relates to a preparation method and application of an immune preparation for preventing and treating novel duck reovirus disease. Background technique [0002] The new type of duck reovirus disease (commonly known as duck hemorrhagic necrotizing hepatitis) is a disease characterized by irregular liver necrosis and hemorrhage that has occurred in Muscovy ducks, semi-muscle ducks and Shelduck ducks in Fujian, Guangdong and Zhejiang provinces in China since 2005. Miscellaneous, myocardial hemorrhage, splenomegaly, plaque necrosis, kidney and bursal hemorrhage, etc. are the main features of the disease. The disease has no obvious seasonality. Ducks, etc.) can occur, and there is a tendency to increase year by year; the age of onset is about 3 to 25 days, of which 5 to 10 days are the majority, the course of disease is 5 to 7 days, the incidence rate is 5% to 20%, and the mortality rate is 2 % to 15%, the younger the age or the higher the mor...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/15A61P31/14C12R1/93
Inventor 陈仕龙陈少莺林锋强程晓霞朱小丽王劭李兆龙
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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