24 results about "Cairina moschata" patented technology

The invention relates to a molecular marker primer related to the feed conversion rate character of Muscovy ducks and application of the molecular marker primer, and belongs to the technical field of molecular detection. According to the molecular marker SNP related to the feed conversion rate character of the muscovy ducks, nucleotide sequences of SNP primers are shown as SEQ ID NO: 1-SEQ ID NO: 2, a first molecular marker is located at the FSTL4 gene GWHBJBF00000015: 17461711 position on the 15 # chromosome in a muscovy duck reference genome KizCaiMos1.0, and a basic group at the position is T or G, and a second molecular marker is located at the position of the FSTL4 gene GWHBJBF00000015: 17461711 position of the 15 # chromosome in the muscovy duck reference genome KizCaiMos1.0. The second molecular marker is located at the FSTL4 gene GWHBJBF00000015: 17461712 position on the No.15 chromosome in the Muscovy duck reference genome KizCaiMos1.0, and the basic group at the position is G or T. The Muscovy duck feed conversion rate screening device has the advantages that Muscovy duck feed conversion rate screening can be achieved, the seed selection accuracy is improved, and the seed selection efficiency is improved.

The invention discloses a kit for detecting a muscovy duck reovirus based on a RAA-CRISPR / Cas12a technology. Belongs to the technical field of Muscovy duck reovirus detection. The invention aims to detect the Muscovy duck reovirus. The invention provides a kit for detecting a muscovy duck reovirus based on an RAA-CRISPR / Cas12a technology. The detection kit comprises an RAA isothermal amplification system and a CRISPR / Cas12a detection system, the method effectively solves the problems that a traditional detection technology is long in time consumption, depends on precise instruments and is complex in operation to detect the pain points on the breeding site.

The invention relates to the technical field of Muscovy duck breeding, and discloses a novel Muscovy duck abnormal behavior automatic identification method, which comprises the following steps: S1, deploying temperature and humidity, ammonia gas and carbon dioxide concentration sensors at the top of a duck shed according to 5-8m < 2 > / duck shed; 4-8 microphone array sound sensors are arranged in the middle of the peripheral wall at intervals of 3-5 meters; high-definition cameras are arranged on the top diagonally in a crossed manner; each Muscovy duck wears an RFID tag and is associated with a biosensor, and body temperature, heart rate and activity data with the precision of + / -0.1 DEG C are collected at the sampling rate of 100-200 Hz. According to the novel muscovy duck abnormal behavior automatic identification method, multiple sensors are deployed in a duck shed to collect multi-dimensional data, visual data are subjected to feature extraction through an improved convolutional neural network, sound signals are processed through a Mel filter bank, and physiological data calibrate individual differences based on historical data; the multi-modal data are aligned through a dynamic time warping algorithm and then fused through an adaptive weighting model, abnormal behavior characteristics are comprehensively captured, similar behavior modes are effectively distinguished, and the recognition accuracy is improved.

The invention relates to the technical field of sheldrake breeding, in particular to a purification breeding method of breeding sheldrake in the middle mountain. Comprising the following steps: step a, duckling selection; b, feeding by adopting a purification feeding method; c, hatching the Zhongshan sheldrake in the step b to lay a first generation of little duck breeds; step d, feeding the first-generation duckling breed in the step c by adopting the feeding method in the step b, and hatching to lay a second-generation duckling breed; step e, feeding the second-generation duckling breed in the step d by adopting the feeding method in the step b, and hatching to lay a third-generation duckling breed; f, feeding the third-generation duckling breed in the step d by adopting the feeding method in the step b, and hatching to lay a fourth-generation duckling breed; the breeding sheldrake in the Zhongshan is obtained. The fifth-generation sheldrake produced by the fourth-generation purified breeding ducks in a common feeding mode basically conforms to the appearance characteristics, the weight and the body size of the Zhongshan sheldrake.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the egg yield character of Muscovy ducks and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, and Cgt exists in the 62nd basic group from the 5'end of the sequence; t mutation. A primer pair and a KASP primer group for amplifying and detecting the SNP molecular marker, wherein the sequences of the primer pair and the KASP primer group are as shown in SEQ ID NO: 2-6; the SNP molecular marker, the primer pair, the KASP primer group and the like are applied to auxiliary identification and breeding of high-egg-yield muscovy duck varieties. The CDC20 gene SNP molecular marker related to the Muscovy duck egg production traits is explored in Muscovy ducks for the first time, a new molecular marker is provided for molecular marker-assisted selective breeding, and a scientific basis is provided for breeding of new Muscovy duck strains with excellent reproduction traits; the CC genotype egg yield of the site is higher than that of the CT genotype and that of the TT genotype, and high-egg-yield Muscovy duck individuals can be quickly screened through early genotyping.

The invention discloses an intelligent monitoring and evaluating system, method and equipment for muscovy duck embryonic development, and belongs to the technical field of poultry incubation. The system comprises: a database storing standard embryo images associated with specific incubation parameters; the image acquisition module is used for acquiring a real-time embryo image; the image intelligent comparison and diagnosis engine is configured to perform feature comparison on the real-time image and a database standard image and output a quantitative evaluation result and / or a parameter adjustment suggestion; and a result output module. According to the method, quantitative diagnosis of the embryonic development state is realized through the machine learning model, and the incubation parameters can be automatically optimized according to the diagnosis result, so that the problem that the incubation performance is unstable due to the fact that the traditional incubation technology depends on artificial experience and lacks objective standards is effectively solved, and the incubation accuracy and the production efficiency of the muscovy ducks are remarkably improved.

The invention discloses a lemongrass addition feeding method suitable for meat duck on-net house breeding, which comprises the following steps: pretreatment of raw materials, preparation and use of a mixed solution, production of lemongrass fermented grass powder, feeding of the lemongrass fermented grass powder and evaluation of meat duck production performance. The method specifically comprises the following steps: lemongrass mowing operation, rubbing and squeezing operation, preparation of a duck shed spraying mixed solution, preparation of a meat duck drinking water mixed solution, a first fermentation stage, a second fermentation stage, evaluation aiming at the growth speed, detection aiming at the breast muscle oleic acid content and detection aiming at the leg muscle oleic acid content. Through the improvement of the technical scheme, the environment of a duck shed can be improved, the quality of meat ducks can be improved, the breeding cost can be reduced, the production performance of the Muscovy ducks can be remarkably improved by adding medium and high doses of citronella fermented grass meal into the feed, particularly, the feed-gain ratio is remarkably reduced, the content of breast muscle oleic acid is increased, and the economic benefit is increased. The feed additive has remarkable economic and social benefits, and meets the development requirements of modern breeding industry.

The invention discloses a mobile drinking water system for muscovy duck breeding, which comprises a breeding cabin, a ventilation turning system, a drinking water system and a fermentation cabin, and is characterized in that the breeding cabin is fixedly connected with the fermentation cabin, the breeding cabin is fixedly connected with the ventilation turning system, the fermentation cabin is fixedly connected with the ventilation turning system, and the fermentation cabin is fixedly connected with the ventilation turning system. The fermentation cabin is fixedly connected with the drinking water system, the breeding cabin is fixedly connected with the drinking water system, and the ventilation turning system is matched with the drinking water system. The invention relates to the field of agricultural breeding equipment, in particular to a movable water drinking system for muscovy duck breeding. The technical problem to be solved by the invention is to provide the mobile water drinking system for Muscovy duck breeding, which is convenient for Muscovy duck breeding.

The present application relates to the field of muscovy duck breeding, and particularly relates to a muscovy duck breeding method and system based on a multi-modal large model. The method comprises the following steps: collecting multi-modal original data in the process of muscovy duck breeding; pre-processing the collected multi-modal original data; constructing a TwinsYOLO detection model, and training the TwinsYOLO detection model through the pre-processed visual data to output muscovy duck detection results; constructing a Conformer speech processing model, and training the Conformer speech processing model through the pre-processed audio data; constructing a V-Mallard multi-modal interactive decision model, and integrating the pre-processed visual, audio and environmental data and the outputs of the TwinsYOLO detection model and the Conformer speech processing model to generate breeding decisions. The present application is suitable for intelligent breeding of muscovy ducks.

The invention discloses a preparation method and application of a Muscovy duck MAPK1 protein polyclonal antibody, and belongs to the technical field of biology. According to the invention, DNAStar software, AI-assisted protein three-dimensional structure prediction and other bioinformatics methods are adopted to accurately determine the 4th-364th amino acids of MAPK1 as immunogens, and protein expression, purification and antibody preparation methods are optimized to obtain the preparation method: expressing an MAPK1 recombinant protein with an amino acid sequence of SEQ ID NO.2, and immunizing a rabbit with the recombinant protein to obtain the Muscovy duck MAPK1 protein polyclonal antibody. The titer of the obtained polyclonal antibody can reach 1: 102400, and the polyclonal antibody has the advantages of high recognition efficiency and strong specificity. The MAPK1 polyclonal antibody prepared in the invention has high targeting recognition capability at the tissue level, can be used for immunofluorescence visualization research of Muscovy duck ovarian tissue protein expression, and provides key technical support for subsequent function exploration of MAPK1 in follicular development and reproduction regulation.

This invention relates to the field of Muscovy duck body size measurement, specifically to an automatic body size measurement method for Muscovy ducks based on multimodal fusion and parametric mesh reconstruction. This invention simultaneously acquires six channels of data (RGB, depth, and infrared) using two binocular depth cameras (side-view and top-view). Infrared-guided depth completion and dual-view point cloud fusion are then used to obtain a complete point cloud. A multimodal feature encoder extracts fused features, and shape and posture parameters are decoded using depth-sensing attention decoding. A three-dimensional mesh is generated by combining this with a statistical shape model of the Muscovy duck, and posture differences are eliminated through posture normalization. Finally, seven body size parameters are geometrically measured on a standard posture mesh, and body weight is estimated using a volume density model. This invention upgrades body size detection from regression prediction to a three-dimensional reconstruction and geometric measurement paradigm, significantly improving measurement accuracy and consistency. This invention is applicable to Muscovy duck body size measurement.

This invention belongs to the field of Muscovy duck farming technology and provides a device for reducing egg damage in laying boxes of female Muscovy ducks. The device includes a breeding box with multiple ventilation mesh panels installed on its outer wall; a disinfection mechanism comprising a storage cylinder, a water outlet pipe, a spray head, an infrared detector, and a control mechanism. The storage cylinder is installed at the top of the breeding box, and the water outlet pipe is installed at the bottom. This invention, through the disinfection mechanism, allows for convenient and regular disinfection of the eggs in the laying box. Intermittent spraying from the spray head disinfects the eggs, and the low temperature of the disinfectant water helps lower the abdominal temperature of any female Muscovy duck that intends to incubate the eggs, preventing them from suffocating. The disassembly mechanism allows the entire laying box to be easily pulled out, eliminating the need for workers to enter the breeding box to collect and retrieve the eggs.

The invention belongs to the technical field of virus detection, and particularly relates to a PCR (Polymerase Chain Reaction) detection kit for four vertical transmission viruses of waterfowl and application of the PCR detection kit. The kit can rapidly, specifically and simultaneously amplify and diagnose duck hepatitis virus type I (DHV-1), duck Tembusu virus DTMUV, Muscovy duck reovirus MDRV and duck astrovirus DAstV, has high specificity and sensitivity, can accurately and rapidly identify DHV-1, DTMUV, MDRV and DAstV, can complete fluorescent quantitative PCR detection in about 50 minutes, and is simple to operate and practical. The detection period is shortened to 1.5 hours from 6-8 hours of a traditional method, the sensitivity is improved by 10-1000 times, the early diagnosis capability of epidemic diseases is remarkably improved, and the epidemic disease misjudgment rate of a farm is obviously reduced. Abuse of vaccines and drugs is reduced, and sustainable development of the poultry breeding industry is promoted.

The application discloses a long-chain non-coding RNA molecule lnc46198 related to development of sex glands of semi-muscovy ducks in an embryonic period and application thereof. The sequence of lnc46198 is shown as SEQ ID NO. 1. It is found by the application that lnc46198 is specifically expressed in sex glands of semi-muscovy ducks in an embryonic period, and inhibits proliferation of duck embryo fibroblasts and promotes apoptosis of duck embryo fibroblasts. It is shown that lnc46198 may affect development of sex glands of semi-muscovy ducks in an embryonic period by inhibiting cell proliferation activity, lays a foundation for functions of key lncRNAs in differentiation and development of germ cells of semi-muscovy ducks and the like in the process of differentiation and development of sex glands in an embryonic period, and provides a scientific reference basis for an occurrence mechanism of a sterile trait of semi-muscovy ducks.

The invention relates to application of fucoidan polysaccharide sulfate in preparation of an inflammation protective agent and an intestinal flora regulator for young muscovy ducks against goose parvovirus infection. The invention provides application of fucoidan polysaccharide sulfate in preparation of a young muscovy duck anti-goose parvovirus infection preparation. The application specifically comprises an anti-inflammatory protective agent for young muscovy ducks against goose parvovirus infection and a flora regulator for repairing and regulating intestinal flora. The protection effect and mechanism of the fucoidan polysaccharide sulfate on the GPV infected young muscovy ducks are explored, and in-vitro and in-vivo experiments are respectively carried out. Results show that medium and high doses of fucoidan polysaccharide sulfate can significantly reduce the content of pro-inflammatory factors in serum and target organs, recover the level of anti-inflammatory factors and relieve tissue inflammatory damage. Microflora analysis results show that medium and high doses of fucoidan polysaccharide sulfate can reverse intestinal flora diversity decline caused by GPV infection, regulate abundance of dominant phylum such as phylum firmicalis and phylum bacteroides, and enrich beneficial bacteria such as Ackermann muciniphila.

The invention relates to an immortalized muscovy duck embryo fibroblast line and an establishment method and application thereof.The immortalized muscovy duck embryo fibroblast line is immortalized muscovy duck embryo fibroblasts iMDEF / SV40-LT, and the establishment method of the cell line comprises the steps that cloned SV40LT genes are inserted into lentivirus plasmids through homologous recombination, and the lentivirus plasmids are subjected to homologous recombination to obtain the immortalized muscovy duck embryo fibroblast line. According to the present invention, the muscovy duck embryo fibroblasts are transfected by using lentivirus, such that the muscovy duck embryo fibroblasts stably express SV40LT, and puromycin screening is performed to obtain the immortalized muscovy duck embryo fibroblasts, the immortalized muscovy duck embryo fibroblasts (iMDEF / SV40-LT) are successfully constructed by using the SV40LT gene, the immortalized muscovy duck embryo fibroblasts stably grow for more than 50 generations, the fibroblast morphology is maintained, and the muscovy duck embryo fibroblasts can be obtained; the method is free of endogenous viruses, good in safety and free of teratogenesis and senescence signs, and can be used for virus culture and the like.

The application belongs to the technical field of molecular biology, and particularly relates to an application of an Indel marker, a primer and a detection kit in screening and / or identifying Muscovy duck. The application provides an Indel marker, which is located in the 2nd intron of a duck RASEF gene, specifically, a "TTGAGTT" fragment insertion or deletion at 11096bp-11102bp of the gene; the marker can be used to accurately identify Muscovy duck; the application further provides corresponding primers, detection kits and methods for specifically identifying Muscovy duck species. Experiments prove that, by using the technical scheme provided by the application, Muscovy duck can be specifically screened and / or identified from Beijing duck, Obaixing duck, Cherry Valley duck, Liancheng white duck, Xuixian curly duck, Jingjiang curly duck, Shanma duck, Shaoxing duck, Jinyun curly duck, Mianyang curly duck and Jinding duck, and the result is accurate, simple and fast.

The application discloses an FGF2 gene molecular marker related to reproductive traits of Muscovy ducks and application thereof, and belongs to the technical field of biological detection. The application finds that an intron region of the FGF2 gene has a molecular marker significantly related to reproductive traits of Muscovy ducks through analysis of the FGF2 gene, the nucleotide sequence of the molecular marker is shown as SEQ ID NO. 1, and there is a SNP site at the 91th nucleotide of the nucleotide sequence shown as SEQ ID NO. 1, which is CM018622.1:g40203218C>A. Experimental results show that the SNP molecular marker is significantly related to the age of first laying of Muscovy ducks (P<0.05), and the age of first laying of CA genotypes and AA genotypes is significantly smaller than that of CC genotypes (P<0.05). It can be seen that the application provides a new SNP molecular marker for MAS, the molecular marker can accurately identify reproductive traits of Muscovy ducks, and provides scientific data for breeding of Muscovy ducks.

The application discloses a molecular marker related to a breast muscle weight trait of Zhongshan Muscovy duck and application thereof, and belongs to the technical field of molecular marker assisted poultry breeding. The molecular marker is a SNP site located at the 7467101th position of a duck genome 5th chromosome, and a G / A mutation exists, and the genotype is GG, GA or AA. By detecting the genotype of the site, the breast muscle weight trait can be determined: the breast muscle weight of an individual with the GG genotype is significantly higher than that of individuals with the GA and AA genotypes. The application can realize early and accurate selection and breeding of the breast muscle weight of the Zhongshan Muscovy duck at the living stage, avoid slaughter determination, reduce costs, improve genetic gain, and provide a reliable tool for improving the meat performance of the Zhongshan Muscovy duck.

The present invention discloses a ULK1 gene SNP molecular marker related to the reproductive traits of muscovy ducks and its application, belonging to the field of molecular biotechnology. This ULK1 gene SNP molecular marker is located at the 869761 site of the fourth intron of the ULK1 gene on chromosome 16 of muscovy ducks, with a G>A mutation. Through experiments, it was found that the ULK1 gene SNP molecular marker is significantly related to the reproductive traits of muscovy ducks, more specifically, significantly related to the age at first laying of muscovy ducks. Using the ULK1 gene SNP molecular marker of the present invention can assist in breeding muscovy duck strains with excellent reproductive traits, providing a scientific basis for the breeding of muscovy ducks.

The invention relates to a feed for improving the immune performance of ducks. 2%-8% of weed fragrance is added in the formula of the feed, and the thymus development level and the cecum acetic acid level can be improved when the feed is used for feeding the white-feather muscovy ducks, so that the food consumption, the weight and the immunity of the white-feather muscovy ducks are improved, and the production performance of the ducks can be improved.

The invention discloses a goose parvovirus strain adapted to various waterfowl embryo fibroblasts as well as infectious clone and application of the goose parvovirus strain. The genome sequence of the goose parvovirus strain provided by the invention only generates three base mutations in the VP1 protein, resulting in the generation of amino acid mutations at three sites. The JNC23 shows a typical cytopathic effect in the growth of embryo fibroblasts of cherry valley ducks, Jinding ducks, Shaoxing ducks, geese and muscovy ducks. The half tissue cell infection amount of the JNC23 on the poultry embryo fibroblasts reaches 105.6 / ml to 107.6 / ml. The invention further provides a construction and rescue method of the infectious plasmid pJNC23 of the JNC23 strain, and the construction and rescue method can be used for further deeply exploring the mechanism that amino acid point mutation of the encoding protein affects NGPV replication and pathogenicity.

The invention relates to the field of sheldrake breeding, in particular to a sheldrake breeding method and system based on a multi-mode large model. The method comprises the following steps: collecting multi-modal original data in a sheldrake breeding process; the collected multi-mode original data is preprocessed; a TwinsYOLO detection model is constructed, the TwinsYOLO detection model is trained through the preprocessed visual data, and a shelduck detection result is output; a Conform voice processing model is constructed, and the Conform voice processing model is trained through the preprocessed audio data; and constructing a V-Mallard multi-modal interaction decision model, and integrating the preprocessed visual, audio and environmental data and the output of the TwinsYOLO detection model and the Conformer voice processing model to generate a breeding decision. The intelligent sheldrake breeding system is suitable for intelligent sheldrake breeding.

The invention provides a Muscovy duck C-type metapneumovirus whole genome sequencing method based on nanopore sequencing. The detection primer group for the Muscovy duck C-type metapneumovirus comprises 24 pairs of amplification primers, and the specific sequences of the amplification primers are as shown in SEQ ID NO.1-48. The detection primer group provided by the invention can realize relatively uniform coverage of the Muscovy duck C-type metapneumovirus, and 100% region coverage of a genome is realized in nanopore sequencing. The preparation method of the sequencing fragment of the muscovy duck C-type metapneumovirus whole genome is simple, convenient and easy to operate and good in amplification effect, nanopore sequencing has the advantages of sequencing and real-time analysis at the same time, the detection time can be greatly shortened, infection of the muscovy duck C-type metapneumovirus can be rapidly identified and diagnosed, meanwhile, the whole genome sequence of the muscovy duck C-type metapneumovirus is obtained, and the application prospect is wide. The obtained genome sequence can provide a scientific basis for virus traceability, pathogenic variation tracking, novel strain identification, early warning and the like.