186 results about "Cairina moschata" patented technology

The invention relates to a preparation method of Muscovy duck parvo novel vaccines, which comprises the following steps of: using Muscovy duck parvovirus attenuated virus P1 strain (MPV-P1 strain with a preservation number of CCTCC NO:V201013) and Muscovy duck origin goose parvovirus attenuated virus D strain ( GPV-D strain with a preservation number of CCTCC NO:V201014) as seed viruses; proliferating viruses by applying the muscovy duck embryone fibroblast spinner culture technology to obtain cell toxic liquids; mixing the cell toxic liquids according to the proper proportion and freezing a dry protective agent to freeze and dry to research safe and effective Muscovy duck parvo novel vaccines. The young Muscovy ducks are inoculated once so as to prevent and control the Muscovy duck parvovirus infection and the Muscovy duck gosling blast dieases at the same time, thereby the stress reaction of the Muscovy ducks caused by immunization for many times is solved. The invention is suitable for scale production under the GMP (Good Manufacturing Practices) condition, and saves the production cost of the vaccines.

The invention discloses a novel muscovy duck reovirus disease vaccine and an application of the novel muscovy duck reovirus disease vaccine. Firstly, a novel muscovy duck reovirus disease (NDRV)-JM85 plant is inoculated with a 11-day muscovy duck embryo proliferation virus by an allantoic cavity to obtain poison-containing allantoic fluid, or the NDRV-JM85 plant is inoculated with a muscovy duck embryo fibroblast cell monolayer; when cytopathy is above 75%, the cellular poison is harvested; after the virus is properly condensed and is inactivated by formaldehyde, the virus and white oil adjuvant are evenly mixed at a proper ratio to obtain the novel muscovy duck reovirus disease vaccine; and then the novel muscovy duck reovirus disease vaccine is used for preventing the novel muscovy duck reovirus disease. The inactivated vaccine is used for immunizing the breeding duck once, and each feather is applied with 1.0ml, immunization is boosted once in four weeks, and each feather is applied with 1.0ml.

The invention relates to a processing method of a novel flavor Muscovy Duck, which is characterized in that it comprises the steps of slaughtering, cleaning, dry pickling, wet pickling, baking, fermentation, packaging, low-temperature sterilization and the like. The ripened muscovy duck is obtained by the processing method of the invention. Compared with the prior art, the present invention adopts low-temperature roasting process and subsequent fermentation and ripening process. The roasting process greatly shortens the dehydration and drying time in the traditional process. The fermentation and ripening process further matures the meat products and fully develops the flavor of the product. out; the low-temperature sterilization process not only ensures that the nutritional components of the product are destroyed as little as possible, but also ensures the quality of the product; the combination of appropriate anti-corrosion control measures is used to ensure the shelf life of the product; the unique product formula is used to make the product unique. flavor.

The invention belongs to the technical field of livestock breeding and particularly relates to a white muscovy duck shed on-line intelligent monitoring system which comprises an environment index monitoring module, an environment activity video monitoring module, a first wireless transmission module, a central processing unit, a second wireless transmission module, an intelligent management module and an intelligent control module. The intelligent management module comprises a server, a desktop computer, a notebook computer and a mobile phone APP. The intelligent control module comprises a dehumidifier, a fan, a heating device, an illumination adjusting device, a water pump control device and an audible and visual alarm. The environment index monitoring module and the environment activity video monitoring module are connected with the central processing unit through the first wireless transmission module, the central processing unit is connected with the server and the intelligent control module through the second wireless transmission module, and the server is connected with the desktop computer, the notebook computer and the mobile phone APP. The objective is that the problems that a conventional manual management method is adopted for an existing Yongchun white muscovy duck shed, so that the investment on manager is high and a systematic comprehensive monitoring angle is lacked are solved.

The invention discloses a general RT-PCR detection primer for muscovy duck reoviruses and a detection method, and belongs to the technical field of muscovy duck reovirus detection. The general RT-PCR detection primer comprises an upstream primer, namely 5'-CACAGTGCTACCATC-3', and a downstream primer, namely 5'-CTGCTGATCATAATT-3'. The detection method comprises the following steps: RT-PCR amplification is carried out by using the general RT-PCR detection primer for muscovy duck reoviruses and samples RNA to be detected, and if the amplification product comprises 436bp fragments, the samples to be detected comprise the muscovy duck reoviruses. After comparison according to the sigma A gene sequence of the muscovy duck reovirus, the general primer is optimally designed, and the one-step process RT-PCR is optimized further. The detection method not only can detect the MDRV muscovy duck reovirus and the NDRV muscovy duck reovirus at the same time, but also has the characteristics of being good in repeatability, strong in specificity, and high in sensibility.

The invention provides a preparation method of a muscovy duck original goose parvovirus and duck type II adenovirus combined inactivate vaccine. The preparation method is characterized in that targeted process conditions are designed according to the proliferation characteristics and immunogenicity of the muscovy duck original goose parvovirus and the duck type II adenovirus. Concretely, chick embryos are used for proliferating the muscovy duck original goose parvovirus; LMH cells are used for proliferating adenovirus; after being inactivated, the viruses are prepared into safe and effective muscovy duck original goose parvovirus and the duck type II adenovirus combined inactivate vaccine. When being used, the prepared muscovy duck original goose parvovirus and duck type II adenovirus combined inactivate vaccine has the advantages that the antibody generation is early; the protection rate is high. The vaccine can be used for preventing two diseases after once inoculation; the stress response caused by multi-time vaccine inoculation of the muscovy duck can be solved.

The invention relates to the field of poultry breeding, and concretely relates to an artificial insemination method for improving the fertilization rate of mule-duck hatching eggs. Through adopting nutritional regulation techniques such as a technique of carrying out artificial supplemental lighting on male Muscovy ducks, and a technique of adding vitamin E and crude protein levels into feeds, and the like, and complementarily taking synthesis techniques such as semen mixing and deposition, environment temperature and semen deposition frequency control in the process of semen deposition, and the like, the fertilization rate of hatching eggs in mule-duck production is improved. The method disclosed by the invention solves the problem that the existing methods such as methods of raising the crude protein level of daily rations of male Muscovy ducks, or methods of manufacturing proper semen diluent formulas or methods of carrying out timely semen deposition are low in fertilization rate of mule-duck hatching eggs, greatly improves the fertilization rate of mule-duck hatching eggs, and lays an important foundation for the expanded production of mule-ducks.

The invention discloses a breeding method capable of improving the egg yield of muscovy ducks. The breeding method comprises the following steps of early-stage primary election, wherein a high-qualitypopulation are selected in young ducks according to the body-size healthy and strong degree in proportion; first-time egg laying selection, wherein according to the whole-period egg production numberof the previous generation and family information, the breeding values of individuals in the current generation are calculated, and high-egg-yield and high-breeding-value male and female ducks are selected and remain in proportion; egg production determination, wherein when the female ducks start laying, the number of the qualified eggs laid by each female duck individual is recorded; second-timeegg laying selection, wherein according to the egg yield of the female ducks in the current generation and the genealogy information, high-egg-yield and high-breeding-value male ducks and female ducks are selected and remain in proportion; family building, a full-sib family is built by the male ducks and the female ducks in proportion, the next generation is bred, and the above steps are repeated. The breeding method has the advantages that the contradictions between subculture and egg laying selection are well solved, genetic contributions on the egg yield in the later egg laying period aretaken into account, the breeding efficiency is improved, and improvement of economic benefits is facilitated.

The invention relates to a method for preparing muscovy duck feed. The muscovy duck feed comprises the following compositions in percentage by weight: 4 to 6 percent of soybean meal, 2 to 6 percent of maize meal, 10 to 12 percent of sorghum powder, 6 to 8 percent of malt powder, 14 to 18 percent of white fishmeal, 4 to 6 percent of freshwater snail meal, 6 to 8 percent of freshwater shrimp meal, 8 to 10 percent of rice bran, 4 to 6 percent of chicken blood meal, 3 to 5 percent of salvinia meal, 6 to 10 percent of grass seed meal, 2 to 4 percent of fish liver oil, 1 to 3 percent of salt, 1 to 3 percent of psoralea, 2 to 4 percent of pinellia, 1 to 3 percent of unibract fritillary bulb, 2 to 4 percent of cyperus rotunus, 1 to 3 percent of mulberry leaf and 1 to 3 percent of cassia seed. According to the proportion, the powders are taken out, put in a multiplex stirrer and stirred; after being taken out, the powders are put in a ring mould type pulverizer and pulverized; then, the powders are taken out, put in a drier and dried; after being taken out, the powders are put in a ring mould type swelling machine and swelled; after being taken out, the powders are transferred to a ring mould type hard granulator to generate precipitated hard granular feed; then the feed is sifted by a 80-mesh sieve; and the weight of each granule is 0.2 gram, namely the feed can be sealed and packaged according to the needed weight.

The invention discloses a compound feed for Muscovy ducks. The compound feed for the Muscovy ducks comprises an energy feed, a protein source, a calcium source, a phosphorus source, mineral additives, vitamin additives and the like. The compound feed for the Muscovy ducks can meet all nutritional requirements of the Muscovy ducks under artificial feeding conditions, raw materials are easy to obtain, the conversion rate of the feed is high, and commercial ducks after feeding have natural light yellow skin and shanks and can further meet the market demand. In addition, by adopting the compound feed for the Muscovy ducks, the feeding cost of the Muscovy ducks can be reduced, so that the compound feed for the Muscovy ducks has great popularization and application values.

The invention provides a muscovy duck hepatitis virus and a method for preparing a muscovy duck hepatitis virus refined egg yolk antibody by adopting the muscovy duck hepatitis virus. The muscovy duck hepatitis virus strain GS14 is preserved in the China Center for Type Culture Collection on April 27, 2016, and the accession number is CCTCC No: V201623. The method for preparing the muscovy duck hepatitis virus refined egg yolk antibody by adopting the muscovy duck hepatitis virus comprises the following steps: preparing antigens by adopting the muscovy duck hepatitis virus strain GS14; carrying out inactivation on the antigens; preparing antigens for vaccines; preparing inactivated vaccines; and preparing the muscovy duck hepatitis virus refined egg yolk antibody. The muscovy duck hepatitis virus and the preparation method have the beneficial effects that the prepared muscovy duck hepatitis virus refined egg yolk antibody can prevent and treat the white liver disease of the muscovy duck, so that an effective means is provided for preventing and treating the white liver disease of the muscovy duck clinically.

The invention discloses a novel duck reovirus strain NDRV-JM85 with a preservation number of CCTCC NO: V201127. The novel duck reovirus strain NDRV-JM85 is used as a virus seed to inoculate an allantoic cavity of a muscovy duck embryo and obtain a virus-containing allantoic fluid, or inoculate an MDEF cell to obtain cytotoxin when a CPE reaches 75%. The virus liquid is inactivated by formaldehyde and added with a white oil adjuvant, and the mixture is mixed thoroughly to from a safe and effective oil emulsion inactivated vaccine for novel duck reovirus. The vaccine is used to immunize a laying hen three times, with an immunization interval of 21 days and 1.0 ml for each hen at each time. Eggs laid 21-63 days after the three immunizations are gained, and extracted yolk and a proper amount of disinfected normal saline is prepared into a hyperimmune antibody preparation for the novel duck reovirus. A duckling without an NDRV maternal antibody is injected with the antibody preparation at 1-2 days old for 0.5ml / duckling, or a duckling with a maternal antibody is injected with the antibody preparation at 10 days old for 1.0 ml / duckling, so as to prevent and control the novel duck reovirus. The invention applies to large-scale production under a GMP condition.

The invention discloses muscovy duck fodder. The muscovy duck fodder is composed of, by weight, 530-603 parts of secondary corns, 0-160 parts of wheat bran, 0-21 parts of soybean oil, 286.4-318.0 parts of bean pulps 44, 0-20 parts of 65-percent imported fish meal, 8.97-74.77 parts of mountain flour, 12.55-16.32 parts of calcium hydrophosphate, 2.7-3.2 parts of salt, 0-1.21 parts of lysine, 1.22-1.41 parts of methionine and 10 parts of brood time gunk or growing period gunk or egg production period gunk. The feeding stages are divided reasonably, nutritional level setting of all the stages is scientific and economical, the nutrition needs of muscovy ducks in all physiological stages are met, and fodder cost is effectively controlled. The production process is advanced.

The invention provides a duck flavivirus and an inactivated vaccine thereof and belongs to the field of veterinarian lemology. The duck flavivirus is a WR strain of duck flavivirus. The WR strain is inoculated with a Muscovy duck embryo, inactivated by formaldehyde and then added with a white oil adjuvant for emulsification to prepare the inactivated vaccine. The invention proposes to prepare inactivated vaccine against duck flavivirus by use of the WR strain of duck flavivirus for the first time; and through the research and preparation of the vaccine, the defect that the disease cannot be effectively prevented at present in China is solved, and the loss caused by the sharp reduction of eggs laid by each duck due to the current high duck flavivirus morbidity can be effectively avoided. By using the WR strain of duck flavivirus to prepare the inactivated vaccine, the process is simple, and the operation is convenient; and the prepared vaccine has the advantages of good antigenicity and high safety, does not bring any bad influence on external environment, and passes the safety evaluation easily.

The invention discloses a triple vaccine special for Muscovy duck, and the triple vaccine special for Muscovy duck uses Muscovy duck liver white-spot disease attenuated MWCA strain, Muscovy duck parvovirus attenuated P1 strain and Muscovy duck goose-origin parvovirus attenuated D strain as seed viruses, and respectively uses SPF chicken embryo fibroblast or Muscovy duck embryo fibroblast spinner cultivation technology to propagate virus and obtain the cell toxic liquid, and after mixing according to a proper proportion, a freeze-drying protective agent is added for freeze drying, and the triple live vaccine of Muscovy duck reovirus disease, Muscovy duck parvovirus disease and Muscovy duck gosling plague special for Muscovy duck is prepared; the triple vaccine can be used in the Muscovy duck culture region where the Muscovy duck reovirus disease, the Muscovy duck parvovirus disease and the Muscovy duck gosling plague are prevalent, and the purpose for preventing and controlling three diseases above can be realized by one-time immunization.

The invention aims to provide an egg yolk antibody for preventing and curing muscovy duck parvovirus diseases, and the egg yolk antibody is prepared by using muscovy duck parvoviruses the preserving number of which is CGMCC (China General Microbiological Culture Collection Center) No.8504 as an antigen. The egg yolk antibody is characterized in that a muscovy duck parvoviruse YBMDP plant is inoculated with a muscovy duck embryo, a die embryo body and embryo liquid are respectively obtained and are grinded and are subjected to freeze thawing so as to obtain a virus solution, and the oil adjuvant mixing emulsification is added in the virus solution after ultrafiltration concentration and formaldehyde solution inactivation so as to obtain a vaccine. The vaccine is utilized to inoculate a laying hen, eggs are obtained, yolks are separated, and the antibody is obtained after inactivation and extraction and rectification. The prepared antibody can prevent ducklings from the parvovirus infection because of muscovy duck parvoviruses, and the egg yolk antibody has the advantages of high efficiency, good safety, high protection rate and the like.

The invention relates to a muscovy duck semen diluent which comprises the following raw materials: 1.6-2.4g/100mL of sodium glutamate, 0.8-1.2g/100mL of glucose, 0.3-0.6g/100mL of monopotassium phosphate, 0.1-0.3g/100mL of magnesium chloride, 0.6-1.0g/100mL of sodium bicarbonate, 60000-80000IU/100mL of streptomycin, and the balance of distilled water. A preparation method of the muscovy duck semen diluent comprises the steps of adding sodium glutamate, glucose, monopotassium phosphate, magnesium chloride, sodium bicarbonate and streptomycin at a raw material ratio, then adding the distilled water to 100mL, and regulating a PH (Potential of Hydrogen) value to be 6.5-7.2. Tests indicate that muscovy duck semen is diluted by the muscovy duck semen diluent at a ratio of 1:1 and 1:2, the average fertilization rate of hatching eggs is higher than the fertilization rate when the raw semen is diluted by normal saline at a ratio of 1:1 and 1:2, and the muscovy duck semen diluent can be popularized and used in the production process.

The invention discloses a feeding method of fat-liver duck, which comprises (1) performing distant hybridization on large-size muscovy duck and fat Beijing duck by artificial insemination method, breeding hybrid progeny for eight weeks, selecting healthy ducks, pre-feeding for two weeks, and feeding in cage; (2) preparing special feed composed of corn, salt, mineral meal, calcium hydrogen phosphate, premix, and Radix Astragali; and (3) feeding fat-liver duck with pneumatic feeding machine twice each day for 26-30 times. The duck is a health food for preventing cardiovascular diseases and invigorating brain; fat liver has no residue of harmful chemical components, safe and reliable service, high product added value, wide market prospect, and significant benefit; feeding with pneumatic feeding machine can save labor, improve efficiency, and lower breeding cost greatly.

The invention provides a Muscovy duck parvovirus inactivation vaccine which comprises an antigen and a vaccine adjuvant, wherein the antigen is inactivated Muscovy duck parvovirus, and the collection number of the Muscovy duck parvovirus is CGMCC No.8504. The Muscovy duck parvovirus inactivation vaccine provided by the invention is prepared by the steps of screening a Muscovy duck parvovirus YBMDP strain with low toxicity and high immunogenicity; inoculating and collecting the virus liquid; performing ultrafiltration concentration and formaldehyde solution inactivation; adding an oil adjuvant and mixing and emulsifying to obtain the vaccine. The vaccine provided by the invention can improve the antibody level of Muscovy duck, guarantee the maternal antibody level of the filial generation and prevent the duckling parvovirus infection caused by the Muscovy duck parvovirus; the vaccine has the advantages of high efficiency and good safety.

The invention belongs to the technical field of livestock and poultry breeding and particularly relates to a breed protection and selection method of Muscovy ducks. The method includes the steps of firstly, selecting the Muscovy ducks conforming to the variety features of Yongchun Muscovy ducks for cultivation so as to obtain adult male ducks and adult female ducks, selecting and eliminating the Muscovy ducks which do not conform to the variety features of the Yongchun Muscovy ducks from the adult male ducks and the adult female ducks and selecting the population conforming to the variety features of the Yongchun Muscovy ducks through a series of cultivation, selection, eliminating and cultivation; secondly, selecting the adult male ducks and the adult female ducks to build an underlying group; thirdly, building generation zero, to be more specific, in the underlying group, randomly selecting dozens of the adult male ducks and the adult female ducks to form a family, and performing breeding of several families; fourthly, performing subculture breeding, to be more specific, giving wing tags to the newborn ducks in the third step, making pedigree records, and performing mixed-group feeding. By the breed protection and selection method, the problem that the preservation of the Yongchun Muscovy ducks is severely affected due to the fact that existing Yongchun Muscovy ducks raisers blindly import hybridization and improvement is solved.

The invention provides a method for breeding muscovy ducks. The method includes steps of disinfecting breeding eggs; arranging the breeding eggs; hatching the breeding eggs and the like. The method particularly includes that the eggs are cooled once every three days when hatched on the 11th-31st days, egg vehicles are repositioned after the eggs are completely cooled, and the eggs continue to be hatched; egg cooling operation is carried out in wet-cooling or dry-cooling modes, the breeding eggs are primarily illuminated on the tenth days, and the breeding eggs without hatching value are removed; the breeding eggs are secondarily illuminated on the 17th days; tray turning operation is carried out after the 31st-32nd days; ducklings are primarily picked once on the 34th and 35th days and are primarily captured when the duckling rate reaches 10%; ducklings are secondarily picked when the duckling rate reaches 70% at least. The method for breeding the muscovy ducks has the advantages of high duckling rate and adaptability to requirements of large-scale industrial farming.

The invention discloses a Muscovy duck feed, which comprises the following raw materials in parts by weight: 40-50 parts of corn, 20-30 parts of broomcorn, 10-20 parts of wheat, 10-15 parts of peameal, 4-6 parts of rapeseed dregs, 4-6 parts of cottonseed meal, 0.5-1 part of mountain flour, 0.5-1 part of bone meal, 0.2-0.3 part of salt, 5-8 parts of pig plasma albumen powder, 2-4 parts of silkworm excrement, 3-4 parts of fermented rabbit dung, 3-5 parts of eggshell, 0.5-1 part of calcium hydrophosphate, 0.06-0.08 part of methionine, 0.5-0.6 part of lysine, 0.01-0.02 part of threonine, 0.02-0.03 part of grape seed, 0.1-0.2 part of garlic and 3-4 parts of Chinese herbal medicine. The pig plasma albumen powder, silkworm excrement, fermented rabbit dung and other raw materials are added into the formula, so that the palatability and trophism of the feed and food consumption of Muscovy ducks can be improved, and the Muscovy duck feed has the good effects on promoting digestion and improving the feed utilization rate, daily gain of the ducks and economic benefits.

The invention provides a field duck cultivation method. The method simulates a field natural growth state and adopts an orchard stocking mode, a Muscovy duck is placed in each duck flock to enhance the movement amount of the duck flock, natural ingestion is combined with pollution-free feed feeding, and traditional Chinese herb medicines are used to prevent diseases. By the method, field ducks are basically in a wild growing mode, good in meat quality, rich in nutrient, high in survival rate, and defects that field ducks raised in pens are large in density, low in movement amount, loose in meat quality, high in fat content, high in prevalence rate, high in medicine use amount, and the like are overcome.