171 results about "Ovarian tissue" patented technology

Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression or underexpression of at least one nucleic acid biomarker in a body sample, wherein the biomarker is selectively overexpressed or underexpressed in ovarian cancer. The body sample may be, for example, an ovarian tissue sample. The biomarkers of the invention include any nucleic acid molecule that is selectively overexpressed in ovarian cancer, including, for example, MMP-7, PAEP, CA125, HE4, PLAUR, MUC-1, SLPI, SSP1, MSLN, SPON1, interleukin-7, folate receptor 1, claudin 3, inhibin A, inhibin BB, inhibin BA, and PAI-1. Overexpression or underexpression of a biomarker of interest is detected at the nucleic acid level using such methods as real-time PCR and various nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided.

The invention discloses a large ovarian tissue vitrification freezing carrier. The carrier consists of a carrier inner core and an outer sleeve, wherein the carrier inner core is in threaded connection with the outer sleeve, maintaining high airtightness and preventing cross infection of the ovarian tissue; meanwhile, the user operation is also facilitated; the carrier inner core consists of a handheld part, a cap part, a support part and a bearing part sequentially from top to bottom; the bearing part is clamped and fixed by the support part; when in use, a large ovarian tissue is put on an end part of the bearing part, away from the support part; the end part is a large ovarian tissue loading area; experiments prove that by using the carrier disclosed by the invention, the permeation efficiency of a refrigerant is improved; moreover, the operation is simple, the price is low, batch storage of large ovarian tissues is convenient, and the carrier is suitable for promotion and application.

A method for aspirating an oocyte from a human female is provided. The method includes the step of providing at least one ovarian cortical piece, implanting the ovarian cortical piece in the human female at a heterotopic location, and triggering oocyte maturity in the at least one ovarian cortical piece. The method also includes the steps of providing an aspiration needle and retrieving at least one oocyte from the at least one ovarian cortical piece using the aspiration needle.

The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic ovarian cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions containing the nucleic acid molecules, polypeptides, antibodies, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating ovarian cancer and non-cancerous disease states in ovarian, identifying ovarian tissue, monitoring and identifying and/or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered ovarian tissue for treatment and research.

The invention belongs to the field related to biological tissue or cell preservation, and relates to a novel tissue cryopreservation liquid, in particular to a liquid used for cryopreserving tumor tissue or ovarian tissue. The cryopreservation liquid is prepared from a Leibovitz L-15 culture medium, fetal calf serum and 14-18% of DMSO (dimethyl sulfoxide), wherein the Leibovitz L-15 culture medium (Gibco) can be purchased or can be self-made by technicians in the field according to a reported formula; preferably, the content of DMSO is 14-16%, more preferably, 15%. For the novel cryopreservation liquid, preferably, the content of fetal calf serum is 14-18%, more preferably, 14-16%, the most preferably, 15%. The cryopreservation liquid has the advantages of being long in cryopreservation time, high in tumor formation rate and the like, the preparation method is simple, and the raw material source is wide.

The method of obtaining immature oocyte from ovary tissue through separating or extracting for extracorporeal culture includes selecting marrow mesenchyme stem cell MSC of female mouse as the feeder cell or culturing the immature oocyte in the MSC culture liquid. After culturing, the co-culture system has obviously increased estradiol, progestin and luteotopin contents, and over 90 % of the immature oocyte become mature. The method is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

The invention relates to a FFRC strain common carp mature ovarian tissue frozen section making method and belongs to the technical field of tissue sections. Mature ovarian tissues are taken out from a FFRC strain common carp, and a FFRC strain common carp mature ovarian tissue frozen section is obtained after fixation, degreasing, dehydration, embedding, freezing and slicing are performed. Compared with a common general freezing and slicing method, degreasing is performed through pure acetone, excessive fat in a later development period of FFRC strain common carp mature ovaries can be efficiently removed, and accordingly the frozen ovarian section high in quality and clearer in structure is obtained. An important and reliable molecular experiment material is provided for tissue localization and functional study such as immunohistochemistry of FFRC strain common carp mature ovarian genes, and the FFRC strain common carp mature ovarian tissue frozen section making method has the important significance on molecular mechanism study of good economic characters of FFRC strain common carps.

The invention discloses a preparation method for a procambarus clarkii ovarian tissue total protein sample suitable for dimensional electrophoresis. The method comprises the following steps: fully grinding ovarian tissue by using the liquid nitrogen method; precipitating proteins by using the trichloroacetic acid-acetone method, that is, precipitating the proteins by using a precooled acetone solution containing dithiothreitol and trichloroacetic acid and rinsing precipitate with a precooled acetone solution containing dithiothreitol a plurality of times; adding a sample lysate to prepare the high quality procambarus clarkii ovarian tissue total protein sample suitable for dimensional electrophoresis. The preparation method for the sample suitable for dimensional electrophoresis is rapid, reliable, simple and practicable, enables a dimensional electrophoresis pattern with high quality and good experimental repeatability to be obtained and subsequent biological mass spectrometric analysis and identification of the proteins to be convenient, and has a great practical application value.

The invention relates to the field of reproductive medicines, in particular to an ovarian tissue cryopreservation method. The ovarian tissue cryopreservation method comprises the following steps: culturing an ovarian tissue to be frozen by a hyaluronidase solution with the volume concentration of 8-12 percent for 10-15 minutes so as to obtain an enzymolyzed ovarian tissue; adding the enzymolyzed ovarian tissue into a freezing protection agent for prebalancing under the temperature of 3-5 DEG C for 10-15 minutes to obtain a prebalanced ovarian tissue; performing programmed cryopreservation on the prebalanced ovarian tissue to reduce the temperature of the prebalanced ovarian tissue to be minus 150 DEG C, and transferring the prebalanced ovarian tissue into a liquid nitrogen tank for storage. Compared with the common prebalancing time of 30-90 minutes in the prior art, the prebalancing time of the ovarian tissue cryopreservation method is greatly shortened, so that the cytotoxic damage, which is caused by the freezing protection agent, to the ovarian tissue under overlong prebalancing time is avoided.

The described invention provides methods for detecting, diagnosing and treating low-grade ovarian cancer and stage I ovarian cancer by comparing results from serum and ovarian tissue samples with normal controls. An increased level of expression of serine protease, wherein the serine protease is at least 2 selected from the group consisting of kallikrein 6 (KLK6), kallikrein 7 (KLK7), and PRSS8, expressed by subject samples compared to the level of expression of serine protease expressed by normal control samples is indicative of possible early stage ovarian cancer in the subject. Once early stage (I/II) ovarian cancer is diagnosed, the subject is treated with a treatment regimen effective to treat the early stage (I/II) ovarian cancer.

A molecular diagnosis system of ovarian cancers encompasses a detection device configured to obtain a detected value of an expression amount of an apolipoprotein A1 gene in ovarian tissue as a diagnosis subject, a storage device configured to store a normal value of the expression amount of the apolipoprotein A1 gene in normal ovarian tissue, and a determination mechanism configured to determine that the ovarian tissue as the diagnosis subject is clear cell adenocarcinoma when the detected value is lower than the normal value.

The present invention is feeder cell for extracorporeal culture of immature oocyte and features that marrow mesenchyme stem cell MSC of female mouse is used as the feeder cell. The feeder cell is easy to obtain, can secrete several kinds of cell factors and growth factors, promote over 90 % of the immature oocyte to become mature and promote the growth, ovulation and maturing of the ovarian follicles in the ovary tissue block under extracorporeal culture. The present invention is significant in surviving rear endangered species of animals, breeding fine variety of animal, cell biological research of embryo stem cell, etc.

An apparatus and method of use thereof for identifying and monitoring women at risk of developing OSE-derived carcinomas is provided. The apparatus includes an introducer needle configured to be capable of insertion into a female such that a terminal end of the needle is positioned adjacent an ovary of the female, a microendoscope having an optic fiber which is operably insertable into the needle in a manner to enable an image of the ovary to be obtained therethrough, and a tissue removing member operably co-insertable into the needle with the optic fiber therein to enable removal of ovarian tissue cells with minimal deleterious effect to the ovary.

The invention relates to an application of a fingerprint spectrum consisting of microRNAs in the diagnosis and treatment of human ovarian cancer. The fingerprint spectrum consisting of multiple microRNAs can be used for distinguishing ovarian tissue from ovarian cancer tissue very effectively, has high sensitivity and specificity and can be effectively applied to the ovarian cancer diagnosis, tumor grading and prognosis evaluation.

A device for freeze preservation of a human ovarian tissue under liquid nitrogen comprises a pipe body; one end of the pipe body is provided with an opening, the opening is equipped with a sealing cover, the sealing cover and the pipe body can be detachably connected, and a sealed storage space is enclosed by the sealing cover and the pipe body; the inner top surface of the sealing cover is provided with a forking device for forking the human ovarian tissue. The forking device comprises a plurality of fine needles which are arranged in parallel, and fixed ends of the fine needles are vertically fixed on the inner end surface of the sealing cover, free ends of the fine needles extend to the pipe body along the axial direction of the pipe body. The forking device comprises 3 fine needles, the 3 fixed ends of the 3 fine needles are in head-to-tail connection to form a regular triangle, and the circle center of the inner end face of the sealing cover is the center of the regular triangle. The device is simple in structure and easy to use; the forking device can avoid physical damage of blind clipping with forceps under liquid nitrogen on an ovarian follicle enriched cortical part in the ovarian tissue, and also avoids cross contamination with other iatrogenic substances in a liquid nitrogen tank.

This invention provides VEGF-FSH compounds having increased serum half-lives relative to either native VEGF or FSH, in which both VEGF and FSH are biologically active. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject, as well as methods for increasing vascularization in a tissue, particularly in ovarian tissue.

The invention provides a pet ovarian tissue cryopreservation resuscitation reagent which comprises: (1) a cryopreservation resuscitation reagent 1 including 2-7W/V% of lycium barbarum polysaccharide, 6-14V/V% of dimethyl sulfoxide, 2-7V/V% of propylene glycol and 80-90V/V% of an MEM (Memminimum Essential Medium) culture medium; (2) a cryopreservation resuscitation reagent 2 including 8-10W/V% of lycium barbarum polysaccharide, 6-20V/V% of dimethyl sulfoxide, 5-15V/V% of propylene glycol and 70-80V/V% of an MEM culture medium; (3) a cryopreservation resuscitation reagent 3 including 15-20W/V% of lycium barbarum polysaccharide, 6-20V/V% of dimethyl sulfoxide, 5-15V/V% of propylene glycol and 70-80V/V% of an MEM culture medium. The invention further provides a pet ovarian tissue cryopreservation resuscitation reagent group and a pet ovarian tissue cryopreservation and resuscitation method.