13 results about "Ovarian cell" patented technology

The application discloses a doxorubicin scavenger for protecting ovarian fertility and a preparation method thereof, comprising a DNA complex wrapped by a red blood cell membrane, wherein the red blood cell membrane is surface-modified by an anti-Mullerian duct antibody alphaAMH; the doxorubicin scavenger is specifically combined with AMH which is highly expressed in ovarian tissues through alphaAMH, is enriched in the ovarian part in a targeted manner, adsorbs doxorubicin through a DNA fragment in the DNA complex, reduces toxic damage of doxorubicin to ovarian cells, and further protects ovarian fertility. The doxorubicin scavenger can adsorb injected doxorubicin Dox, reduce side effects of doxorubicin Dox on ovarian granulosa cells, and further protect ovarian function and fertility.

The application belongs to the technical field of biology, and discloses a use of antisense nucleotide of piR-bmo-796514 nucleotide for preparing a medicine for inhibiting Bombyx mori nuclear polyhedrosis virus, the antisense nucleotide of the piR-bmo-796514 nucleotide is mainly used for inhibiting replication of the Bombyx mori nuclear polyhedrosis virus in ovary cells of the Bombyx mori, and a sequence of the piRNA nucleotide is shown as SEQ ID NO:1; in addition, the application also discloses the antisense nucleotide of the piR-bmo-796514 nucleotide, and an antisense nucleotide sequence is shown as SEQ ID NO:2.

This invention discloses an S-estrol composition for delaying ovarian aging and its uses, relating to the fields of biomedicine and health products. The S-estrol composition for delaying ovarian aging comprises, by weight, the following components: 1-5 parts S-estrol, 2-8 parts soy isoflavones, 3-10 parts coenzyme Q10, 2-6 parts vitamin E, 4-12 parts vitamin C, 0.01-0.05 parts folic acid, 0.1-0.3 parts zinc source, 0.5-1.5 parts anti-aging protective agent, 50-80 parts carrier, and 10-15 parts purified water. This invention uses high-purity S-estrol as the core, combined with soy isoflavones, coenzyme Q10 and a variety of antioxidant nutrients in a scientific formulation to form a synergistic system for estrogen receptor signal regulation and antioxidant defense. By constructing a complex antioxidant network and introducing novel anti-aging protectants for synergistic effect, the activity of antioxidant enzymes tends to increase and the level of lipid peroxidation tends to decrease, thereby improving the oxidative stress state in ovarian cells as a whole.

The invention discloses a slow-release nutrition preparation for small needle knife guide in an ovarian reflex region and a preparation method of the slow-release nutrition preparation, and relates to the technical field of medical nutrition preparations. 20 to 30 parts of a slow release carrier; 3-6 parts of an absorption enhancer; 45 to 65 parts of a biocompatible auxiliary material; 1-3 parts of a stabilizer; according to the invention, the D-chiro-inositol is helpful for adjusting hormone balance in vivo and promoting ovarian health; glutathione is a potent antioxidant and protects ovarian cells from oxidative damage; the Q10 coenzyme can enhance cell energy metabolism and improve the ovarian function; soybean isoflavone is phytoestrogen and can simulate the action of estrogen of a human body, the core nutritional ingredients are combined with a slow release carrier prepared from chitosan, sodium alginate and a medicinal fungal polysaccharide compound, slow release of nutrient substances is achieved, action time is prolonged, bioavailability of the nutrient substances is improved, the substances are combined, and the slow release effect is achieved. The nourishing and long-acting protection on the ovary reflex region are realized.

The invention discloses application of bilobalide in inhibiting migration and invasion of ovarian cancer cells, and relates to the technical field of biological medicines. Application of bilobalide in preparation of drugs for inhibiting migration and invasion of ovarian cancer cells; the concentration of the bilobalide is 5 [mu] M to 40 [mu] M. In the application of the bilobalide, the bilobalide shows an obvious growth inhibition effect on ovarian cancer cells and has no obvious influence on the survival rate of normal ovarian cells; in a concentration range of 5-40 [mu] M, bilobalide shows dose dependence on activity inhibition of SKOV3 and ID-8 cells, but has small influence on IOSE80 cells, and shows a good safety window; compared with a traditional chemotherapeutic drug, the bilobalide as a natural compound has better biocompatibility, and is expected to be capable of remarkably relieving common toxic and side effects such as myelosuppression and liver and kidney function impairment in clinical application.

This invention discloses an inhibitor for controlling fall armyworm and its application, relating to the field of insect control technology. The inhibitor uses rapamycin or AZD-8055 as the main active ingredient and DMSO or water as the solvent. Rapamycin is a macrolide immunosuppressant produced by *Streptomyces hygroscopicus*, and AZD-8055 is an ATP-competitive TOR kinase inhibitor. The effective working concentration of rapamycin is 0.1 μM to 50 μM; the effective working concentration of AZD-8055 is 0.1 μM to 50 μM. The inhibitor causes morphological changes or death in the fall armyworm ovarian cell line Sf9. The inhibitor provided by this invention uses rapamycin or AZD-8055 as the active ingredient, targets the insect TOR signaling pathway, has no cross-resistance with existing chemical pesticides and Bt protein, and can effectively respond to resistant populations, solving the problem of pesticide resistance in fall armyworm.

The application discloses a flower glue peptide with ovary protection and whitening effects and a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing flower glue of a sea bass in a certain proportion, homogenizing the flower glue by using a homogenizer, sterilizing the flower glue by using a high-pressure sterilization pot, performing fermentation treatment on the flower glue by using Lactobacillus mucosus JIAN, adjusting the pH of the treated flower glue solution by using hydrochloric acid, adding acid protease of Aspergillus niger for enzyme hydrolysis, and then adding aminopeptidase for enzyme hydrolysis, so as to obtain a flower glue peptide solution. The flower glue peptide prepared by the application has significant ovary protection and whitening functions. The whitening function is mainly embodied in tyrosinase inhibition activity, and the ovary protection function is embodied in hydrogen peroxide-induced ovary cell survival rate and anti-mullerian hormone content, so that the economic value of the sea bass by-product is greatly improved. In addition, the flower glue peptide also has the characteristics of weak fishy smell, low fat content and small molecular weight.

The invention provides a composition for preventing and treating ovarian senescence and a preparation method and application thereof, and belongs to the technical field of ovarian senescence prevention and treatment. The composition for preventing and treating ovarian aging provided by the invention is prepared from radix rehmanniae praeparata, fructus lycii, poria cocos, astragalus membranaceus and perilla frutescens. According to the method, multidisciplinary technologies such as data mining, network pharmacology, weighted gene co-expression network analysis (WGCNA) combined machine learning and molecular docking verification are integrated; the multi-component-multi-target-multi-channel action mechanism of the medicinal and edible composition consisting of prepared rehmannia root, wolfberry fruit, poria cocos, astragalus membranaceus and purple perilla for preventing and treating ovarian aging is systematically illuminated. According to the medicinal and edible composition disclosed by the invention, the release of SASP (senescence-related secretory phenotype) senescence-related factors is slowed down by regulating and controlling a PI3K-Akt / SIRT1-TP53 signal axis, so that the senescence of ovarian cells is delayed, the effect of resisting ovarian senescence is exerted, and a theoretical basis is provided for the research of a modern pharmacological mechanism.

The preparation method comprises the following steps: uniformly mixing perch fish maw with water according to a certain proportion, homogenizing by using a homogenizer, sterilizing by using a high-pressure sterilization pot, carrying out fermentation treatment by using lactobacillus mucus JIAN, then adjusting the pH value of the treated fish maw solution by using hydrochloric acid, and carrying out freeze-drying to obtain the fish maw peptide with ovary protection and whitening effects. Aspergillus niger acid protease is added for enzymolysis, then aminopeptidase is used for enzymolysis, and a fish maw peptide solution is obtained. The fish maw peptide prepared by the invention has remarkable whitening and ovarian protection functions, the whitening function is mainly embodied from tyrosinase inhibitory activity, the ovarian protection function is embodied by measuring cell survival rate and anti-mullerian hormone content by inducing ovarian cells through hydrogen peroxide, and the economic value of perch byproducts is greatly improved. In addition, the fish maw peptide also has the characteristics of weak fishy smell, low fat content, small molecular weight and the like.

The invention belongs to but is not limited to the technical field of gene engineering, and particularly relates to a preparation method and application of unlabeled sebastes schlegeli IGF2 recombinant protein, and by detecting the IGF2 expression level in different development stages of ovary, it is found that IGF2 has an important regulation effect on fertilized ovary matrix and embryo. The cDNA of sebastes schlegeli is used as a template for PCR amplification to obtain the sebastes schlegeli IGF2 mature peptide, and the amino acid sequence of the sebastes schlegeli IGF2 mature peptide is as shown in SEQ ID NO: 2. The recombinant protein comprises the IGF2 mature peptide, a His-Sumo tag connected with the N end of the IGF2 mature peptide, and a connecting peptide fragment between the His tag and the Sumo tag, and the amino acid sequence is shown as SEQ ID NO: 1. The invention also provides application of the sebastes schlegeli IGF2 mature peptide recombinant protein in promoting proliferation of primary ovarian cells and development of germ cells of sebastes schlegeli.

The invention provides a fermented dandelion composition with an ovarian cell repairing function as well as a preparation method and application thereof, and relates to the technical field of biological medicines and functional foods. The fermented dandelion composition with the ovarian cell repairing function is prepared by conducting composite enzymolysis and four-strain synergistic fermentation on whole dandelion leaf roots, enzyme preparations adopted in the composite enzymolysis are cellulase, pectinase and protease, and the weight adding ratio of the cellulase to the pectinase to the protease is 3: 2: 1; the strains adopted in the four-strain synergistic fermentation are aspergillus niger, saccharomycetes, lactic acid bacteria and bacillus subtilis, and the volume inoculation ratio is 2: 1: 1: 1. By innovating a four-bacterium fermentation process, the micromolecular protein content of the product reaches 12.8 g / 100g, which is 8.3 times that of the original medicinal materials, and is obviously higher than that of a single yeast fermentation product; meanwhile, dandelion polysaccharide is enriched, new-structure active substances such as AIP-1 and EPIG-1 are newly added, the oral bioavailability of rats reaches 68.9% and is far better than that of unfermented and single-strain fermented products, and the conversion and absorption efficiency of active ingredients is greatly improved.

The application relates to a sea cucumber egg oligopeptide composition for improving female AMH values and ovarian function and an application thereof, and belongs to the medical field. The sea cucumber egg oligopeptide composition comprises, in terms of weight parts, 15-25 parts of sea cucumber egg oligopeptide, 10-15 parts of mussel oligopeptide and 10-20 parts of a medicinal and edible polyphenol extract. The sea cucumber egg oligopeptide has the effects of improving hormone levels such as AMH, improving energy metabolism and oxidative stress levels of oocytes and ovarian granulosa cells and promoting ovarian stem cell proliferation. The medicinal and edible polyphenol extract has the effects of reducing chronic inflammation of ovarian cells, improving blood supply of ovarian tissues, increasing the organ coefficient of the uterus, and relieving immune damage of the ovary, and the absorption rate of the medicinal and edible polyphenol extract is significantly improved compared with direct intake of an existing traditional Chinese medicine formula. The components improve ovarian function from different aspects, and the components synergistically produce an improvement effect on ovarian reserve which is superior to that of intake of single or few components, and the components are safe and have no toxic side effects.

The invention relates to the technical field of biological medicines, and discloses a preparation method of 3D amniotic mesenchymal stem cells for improving premature ovarian failure, which comprises the following steps: separating amniotic mesenchymal stem cells; carrying out subculture on the amniotic mesenchymal stem cells; the cod collagen solution and the sodium alginate aqueous solution are mixed and injected into a CaCl2 solution for crosslinking, and a three-dimensional culture scaffold is obtained; and putting the three-dimensional culture scaffold into a 6-pore plate, adding the amniotic mesenchymal stem cell suspension, and standing to obtain the 3D amniotic mesenchymal stem cells. According to the preparation method of the 3D amniotic mesenchymal stem cells for improving the premature ovarian failure, the cod collagen and the alginate are used as a three-dimensional cell scaffold, the cod collagen promotes stem cell surface integrin combination, the adhesion effect of the mesenchymal stem cells is improved, the stem cell surface integrin binding rate is increased, the stem cell surface integrin binding rate is increased, and the stem cell surface integrin binding rate is increased. Meanwhile, the pore structure of the three-dimensional cell scaffold simulates an ovarian extracellular matrix, promotes the mesenchymal stem cells to secrete growth factors and activates primordial follicles, so that the premature ovarian failure is improved.