67 results about "Premature aging" patented technology

The invention provides a strain of lactobacillus plantarum YS-AG23 with high antioxidant activity, and a rhizoma polygonati and radix angelicae sinensis fermented product obtained by fermenting rhizoma polygonati and radix angelicae sinensis with the strain of lactobacillus plantarum YS-AG23. In a D-galactose induced premature ovarian failure mouse model, the fermentation product can regulate up the levels of antioxidant and stress protection genes SOD2 and HO-1 and an anti-aging gene SIRT1 by regulating down the expression of a key gene AGER of an inflammation and oxidative stress pathway, so that the oxidative stress and inflammatory response of model mouse ovarian tissues are remarkably reduced; meanwhile, the serum hormone level can be specifically adjusted, the serum estradiol (E2) level can be increased, the follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels can be reduced to be close to the normal range, the expression of anti-mullerian hormone (AMH) and hormone regulatory gene LHR of ovarian reserve markers can be up-regulated, the proliferative activity and estrogen secretion function of follicular granulosa cells can be improved, and the effect of preventing ovarian reserve is achieved. The wet weight of atrophic uterus and ovary of the model mouse is remarkably increased, uterine and ovary atrophy is effectively reversed, and the ovarian reserve function of the D-galactose induced premature ovarian failure model mouse is recovered. Besides, in an H2O2-induced human dermal fibroblast aging model, the yeast can improve the activity of aging cells in a concentration-dependent manner and reduce the level of reactive oxygen species (ROS) in the cells; in addition, by up-regulating the expression of a collagen synthesis key gene COL1A1 and down-regulating the expression of a cell senescence marker gene p16, skin cell senescence caused by oxidative stress can be effectively improved. In conclusion, the polygonatum sibiricum and angelica sinensis leavening can synchronously improve premature ovarian failure and skin cell senescence caused by oxidative stress by accurately regulating and controlling oxidative stress, inflammatory response and tissue function related genes, and has wide application potential.

The application discloses application of NCOA7 circular RNA nanoliposome in preparation of a drug for treating premature ovarian failure or ovarian reserve function decline, and belongs to the technical field of biological medicine.The present application proves that nuclear receptor coactivator 7 (NCOA7) can relieve ovarian aging and significantly improve the ovarian function and fertility of DOR patients.The present application finds that NCOA7 circular RNA nanoliposome treatment can significantly improve the NCOA7 protein expression level of DOR female ovarian granulosa cells, improve the aging phenotype and growth rate of the DOR female ovarian granulosa cells, and has no significant influence on the biological characteristics of the ovarian granulosa cells, thereby providing a new scheme for rescuing the ovarian function and improving the fertility of DOR women.

The present invention provides a novel promoter for lipid synthesis in sebaceous gland cells. By using the novel promoter for lipid synthesis in sebaceous gland cells, a skin barrier damage repairing agent and cosmetics with a skin barrier damage repairing function can be prepared, so that cosmetics for repairing skin barrier damage of at least one type selected from skin dryness, premature skin aging, skin aging, skin pruritus, and senile sebaceous deficiency eczema caused by hypofunction of sebaceous glands and / or reduced sebum secretion can be developed.

The application discloses application of a novel peptide UHP in preparation of a medicine for treating and / or preventing delayed aging, and belongs to the technical field of biological medicines.The novel peptide UHP is composed of 37 L-type amino acids including a transmembrane peptide HIV-TAT.It is proved by cell experiments that the novel peptide UHP significantly improves the aging phenotype of a HGPS cell model simulated by overexpression of progerin and a replicative senescent human normal fibroblast cell, reduces the SA-beta-gal positive cell proportion and the expression of SASP factors, and increases the expression levels of cell proliferation markers Ki67, Lamin B1 and Cyclin A2.It is proved by animal experiments that the novel peptide UHP can improve the progeria phenotype, body weight and exercise capacity of a progeria mouse, relieve fibrosis in multiple tissues, improve the skin aging phenotype, and prolong the survival period of the progeria mouse, thereby providing a new medicine selection for clinical treatment of aging-related diseases.

The invention discloses an extract for promoting health and application thereof, and belongs to the technical field of medical and edible dual-purpose and traditional Chinese medicine health care, and the extract is a special extract obtained from beautiful millettia roots. The beautiful millettia root extract exerts new activity for promoting female health through a new mechanism, and the clinical effect is obviously improved. Experimental research proves that the extract promotes self-renewal of female germline stem cells and directional differentiation of the female germline stem cells into oocytes by promoting expression of Notch1, HES1 and HES5 in a Notch signal channel and regulates the female progestational hormone level, and shows the effects of promoting and recovering development of a second sexual character and preventing symptoms of polycystic ovarian syndrome on ovarian development disorder, and has a good application prospect. The radix millettiae speciosae extract is capable of promoting female physical development and maturation, delaying premature ovarian failure, relieving female depression and anxiety states and keeping female health, belongs to a new target and new mechanism, and makes up the mechanism blank of the radix millettiae speciosae extract in the aspect of keeping female health. The application has innovativeness.

This invention relates to the field of anti-aging drug technology, specifically to the application of small molecule compounds in the preparation of anti-aging drugs. These small molecule compounds competitively block the interaction between KDM6A and the vacuolar ATPase V0 domain subunit ATP6V0C by targeting a specific small molecule binding pocket of the histone demethylase KDM6A, thereby inhibiting the demethylation activity of KDM6A on lysine residue 36 of ATP6V0C. These small molecule compounds can effectively downregulate aging-related β-galactosidase activity, improve cell cycle arrest, and reverse the senescent phenotype of nerve cells induced by the environmental pollutant GenX, making them particularly suitable for combating premature aging of nerve cells induced by perfluorinated and polyfluoroalkyl compounds such as GenX. This technical solution can solve the technical problem of the lack of drugs with clearly defined components, a clear mechanism of action, stable efficacy, and the ability to specifically alleviate aging in existing technologies, and has ideal prospects for widespread application.

The invention relates to the technical field of anti-aging drugs, in particular to application of a small molecule compound in preparation of anti-aging drugs. The small molecule compound competitively blocks the interaction between the KDM6A and a vacuolar ATP enzyme V0 structural domain subunit ATP6V0C through a specific small molecule binding pocket of targeted histone demethylase KDM6A, and further inhibits the demethylation activity of the KDM6A on 36th lysine of the ATP6V0C. The small molecule compound can effectively down-regulate the activity of senescence-related beta-galactosidase, improve cell cycle arrest and reverse nerve cell senescence phenotypes induced by environmental pollutant GenX, and is especially suitable for resisting nerve cell premature senescence caused by perfluorinated and polyfluoroalkyl compounds such as GenX. According to the technical scheme, the technical problem that drugs which are definite in component, clear in action mechanism, stable in curative effect and capable of pointedly relieving senescence are lacked in the prior art can be solved, and ideal application and popularization prospects are achieved.

The present application provides a kind of transcription factor PpeNAC33 for regulating plant growth and development and chlorophyll degradation, nucleotide sequence is as shown in SEQ.ID.NO.2, amino acid sequence is as shown in SEQ.ID.NO.3.The present application also provides the vector based on the above-mentioned transcription factor, engineering strain.Meanwhile, the application of the above-mentioned transcription factor PpeNAC33 in regulating plant growth and development and chlorophyll degradation is provided.The present application finds a NAC transcriptional regulatory factor PpeNAC33 in the transcriptome analysis of seed abortion peach fruit.Studies have shown that the transcription factor is a key transcription factor for regulating plant growth and development and chlorophyll degradation, and its expression can be accurately regulated by space-time and expression amount, which can be used to realize the accurate control of plant growth process and avoid the occurrence of plant fruit premature aging.

This invention discloses a Polygonatum sibiricum vesicle preparation for treating premature ovarian failure, its preparation method, and its application. The preparation method includes: cleaning Polygonatum sibiricum, juicing, filtering to remove residue, and collecting the juice; subjecting the juice to gradient centrifugation to remove residual plant tissue, plant cells, and plant cell debris; then using an extraction reagent for stepwise precipitation, collecting the precipitate, and resuspending it to obtain the final product. The Polygonatum sibiricum vesicle preparation prepared by this invention can be efficiently taken up by ovarian granulosa cells. It effectively treats premature ovarian failure by activating the Nrf2 signaling pathway, upregulating antioxidant enzyme expression, scavenging reactive oxygen species, restoring mitochondrial membrane potential, inhibiting apoptosis, and restoring the expression levels of estrogen, follicle-stimulating hormone, and anti-Müllerian hormone. The preparation method of this invention is standardized, biocompatible, and safe, and has broad clinical application prospects.

The application relates to the technical field of plant breeding, and discloses a cultivation method of a powdery mildew-resistant plant and application of a mesophyll-specific expression vector, which comprises the following steps: transforming the mesophyll-specific expression vector into the powdery mildew-resistant plant, so that the mesophyll cells in the powdery mildew-resistant plant can specifically express a powdery mildew-resistant gene MLO; and the powdery mildew-resistant plant is obtained by making the powdery mildew-resistant gene MLO in the plant be mutated. According to the application, the mesophyll-specific expression vector is introduced into the powdery mildew-resistant plant (the plant has a premature aging phenomenon) with the function of the powdery mildew-resistant gene MLO being inactivated, so that the mesophyll cells in the plant can specifically express the powdery mildew-resistant gene MLO, and the plant which is resistant to powdery mildew and has no growth defects is obtained. The cultivation method provided by the application can eliminate the premature aging phenotype of the existing powdery mildew-resistant plant with premature aging, successfully decouples the mlo-mediated disease resistance and growth demand, and provides a new perspective for further analyzing the MLO-mediated disease mechanism research.

WATER-SOLUBLE EXTRACT FROM A PLANT OF THE PRUNUS SPECIES TO PREVENT THE DELETE EFFECTS OF THE EXPOSOME. The present invention relates to the use of a flower extract from a plant of the Rosaceae family and the Prunus genus, or a composition thereof, to protect the skin from the deleterious effects of the exposome, including disruption of the barrier function, neurogenic inflammation, irritation, and oxidative stress. The invention is thus intended to prevent the clinical signs of premature aging, including the appearance of hyperpigmentation spots responsible for loss of skin tone uniformity and skin hyperreactivity.

The invention belongs to the technical field of medicines, and particularly relates to application of a licorice extract in preparation of a medicine for treating premature ovarian failure. On the basis of network pharmacology prediction and animal experiment verification, through experimental exploration, it is clear that diammonium glycyrrhizinate can treat premature ovarian failure by improving ovarian tissue energy metabolism, reducing the oxidative stress level and inhibiting ovarian cell apoptosis, and also can treat premature ovarian failure by improving the ovarian growth and development microenvironment. According to the present invention, the growth and development rate of ovarian granular cells and membrane cells is improved, the sufficient quantity of granular cells and membrane cells are ensured to secrete female progestational hormone, the damaged ovarian tissue is further protected, the new strategy and hope are provided for the treatment of premature ovarian failure, and the pharmaceutical composition is expected to become the safe and effective treatment drug.

The application discloses application of cell endogenous metabolites in preparation of medicines for treating and / or preventing aging. The cell endogenous metabolites are any one or combination of phosphocreatine, hypotaurine and / or phosphoethanolamine. The application finds that phosphocreatine, hypotaurine and phosphoethanolamine can effectively restore cell growth, reduce cell SA-beta-Gal staining intensity, improve cell mitochondrial function and increase ATP yield, restore cell mitochondrial membrane potential, alleviate damage caused by cell oxidative stress and premature aging, and also prolong the life of nematodes and restore exercise capacity in the middle of aging. Meanwhile, the three metabolites have a synergistic effect, and by adjusting the matching concentration of the three metabolites, the same cell protection effect can be achieved by mixed administration. Since the endogenous metabolites are accurate, efficient and safe, the three cell endogenous metabolites provided by the application can be used for preparing anti-aging medicines.

The invention belongs to the cross technical field of regenerative medicine and traditional Chinese medicine, and discloses a composition for treating premature ovarian failure and a preparation method and application thereof. The composition comprises adult stem cells, traditional Chinese medicine active components consisting of astragaloside and salvianolic acid B in a molar ratio of (1: 1)-(4: 1) and a biodegradable slow-release carrier, and double intervention of'cell repair-microenvironment regulation and control 'is realized through a synergistic strategy of'pretreatment of stem cells by traditional Chinese medicines and targeted slow-release delivery'. Experiments prove that the composition can enable the estrus cycle recovery rate of premature ovarian failure model animals to reach 70%-90% and the number of follicles to be increased by 6-8 times, is obviously superior to the existing single therapy, has a clear mechanism (activating a PI3K / Akt pathway and inhibiting a TGF-beta1 / Smad pathway), and has outstanding clinical transformation value.

The application discloses a biological age evaluation method, comprising the following steps: obtaining a key protein index of a person to be evaluated, inputting an improved biological age evaluation model I, and obtaining the biological age of the person to be evaluated; the improved biological age evaluation model I is obtained based on an elastic network regression algorithm by taking proteomic data and body composition age of an individual as training data. The application also discloses a construction method of the model, i.e., a two-step method. The application allows a user to calculate the biological age according to the proteomic data, realizes personalized monitoring of muscle aging state, and has great guiding significance in identifying individuals with early muscle function decline and formulating personalized and accurate anti-aging schemes.

The invention provides a diagnostic application of NDUFS5 in premature ovarian insufficiency disease, the NDUFS5 peripheral blood expression level (mRNA) is detected to have a significant difference between POI and a control group, the difference is verified by qPCR in an independent queue, and the result shows that the expression of the NDUFS5 gene in the premature ovarian insufficiency group is significantly reduced compared with the control group, and the NDUFS5 gene has a significant effect on the premature ovarian insufficiency disease. The trend is consistent with that of transcriptome sequencing, and meanwhile, training and verification data are combined to prove that the NDUFS5 can be applied to distinguishing normal ovarian functions and ovarian insufficiency. The kit can be used for diagnosis of premature ovarian insufficiency (premature ovarian failure and ovarian aging), solves the clinical dilemma that diagnosis is delayed by means of existing means for POI diseases in the prior art, enables high-risk groups to be capable of early prediction or early warning, and avoids the risk that irreversible fertility is lost in advance due to the fact that the optimal period of early intervention is missed.

The application relates to a method for establishing an early ovarian failure rat model, which comprises the following steps: taking healthy rats, and performing gavage on the rats according to a dosage of 10-20 mg / Kg of a phytostilbene preparation, wherein the dosage is the ratio of phytostilbene to the weight of the rats, and obtaining the early ovarian failure rat model after four days of administration. The early ovarian failure rat model is obtained by performing gavage on the rats after the phytostilbene preparation, the model is simple to establish, and the model establishment time is short. Through ovarian pathological observation, it can be seen that the simulated animal model has obvious ovary tissue damage, follicular granulosa cell destruction and apoptosis, and follicle atresia and other symptoms. The obtained model has good stability and repeatability. The phytostilbene is used to successfully establish the early ovarian failure rat model, lays a good foundation for establishing a stable early ovarian failure model for basic research, and provides a reliable method for the construction of the early ovarian failure model.

The invention relates to the technical field of microorganisms, and particularly discloses a new application of a known strain lactobacillus plantarum AL4510, namely an application of the known strain lactobacillus plantarum AL4510 in preparation of a product for improving premature ovarian failure. Experiments show that the lactobacillus plantarum AL4510 can improve the ovarian index and function, maintain the normal development of follicles, recover the disordered estrogen level, and relieve the oxidative stress injury of ovarian and colon tissues. Particularly, the improvement effect of the lactobacillus plantarum strain is obviously superior to that of other lactobacillus plantarum strains. Therefore, the lactobacillus plantarum AL4510 and the microbial agent thereof have a good application prospect in preparation of drugs or functional foods for preventing or relieving premature ovarian failure (especially induced by repeated superovulation).

The invention belongs to the technical field of biology, and discloses a preparation method and application of a stem cell preparation for repairing ovarian function premature senility, the preparation method comprises the following steps: adding stem cells and vascular endothelial growth factors into a serum-free medium for culturing to obtain a proliferated stem cell solution; the amino component, the aldehyde component and the connexin are added into normal saline according to a preset proportion to be mixed, hydrogel is obtained, and an imine group formed by amino in the amino component and aldehyde in the aldehyde component is broken under the preset stress condition; and stirring and mixing the hydrogel and the proliferated stem cell solution to obtain the stem cell preparation. By means of the preparation method, the homing rate of the stem cells in the injection process for treating the premature ovarian failure is increased, and the loss rate of the stem cells is reduced.

The invention provides application of overexpressed IGF1R in preparation of a medicine for treating kidney IRI related symptoms, and relates to the technical field of biological medicine. The overexpressed IGF1R can inhibit the symptom of the cell premature senility induced by IRI in the transplantation process, a carrier of the overexpressed IGF1R is designed on the basis of the overexpressed IGF1R, and an IGF1R related agonist such as diflurosil or L-leucine can effectively inhibit the cell premature senility induced by the IRI; a new target spot and a new strategy are provided for clinically diagnosing and treating cell senescence caused by kidney IRI due to organ transplantation.

The present invention relates to a method for identifying the aging degree of a subject or a tissue or organ thereof, and for evaluating the effect of anti-aging interventions. Specifically, provided is a method for identifying the aging degree of a subject or a cell, tissue or organ thereof, or for evaluating the biological age of the subject or diagnosing premature aging disorders, which method predicts the aging status of a tissue or organ of a subject on the basis of the levels of DNA methylation, gene transcripts, proteins or metabolites. The present invention further relates to the use of metformin in the preparation of a drug for treating, preventing or delaying aging-related diseases or conditions in primates, preferably humans.

The invention discloses a premature ovarian failure rat animal model building method based on enterovirus group transplantation, and particularly relates to the technical field of animal model building. According to the invention, the enterovirus group of a premature ovarian failure patient is subjected to in-vitro extraction and purification, and then is transplanted into a female rat, so that a new experimental model is provided for researching the interaction between the enterovirus group and the premature ovarian failure; meanwhile, a new way is opened up for further revealing the research on the complex relation between intestinal microorganisms and female reproductive health. In addition, the model is expected to provide an experimental basis for developing a novel treatment strategy for premature ovarian failure. The method aims to deeply explore the relationship between the enterovirus / bacteriophage group and female reproductive system diseases, especially premature ovarian failure.

The invention discloses an osmanthus fragrans extract for preventing premature ovarian failure and a preparation method and application thereof.The extract is prepared from osmanthus fragrans through microwave fixation, gradient temperature weak acid extraction, membrane separation and concentration, low-temperature drying and other pure physical processes, effective components can be prevented from being damaged, the process is stable, green and efficient, the active components are definite, and the preparation method is simple. Wherein the sum of the contents of the salidroside, the verbascoside and the ligustroside is not less than 20%. Animal experiments show that AMH, FSH and LH hormone levels in mice suffering from premature ovarian failure induced by cyclophosphamide can be remarkably improved, the ovarian function can be effectively improved, all index components including salidroside, verbascoside and ligustrum glycoside have a synergistic interaction effect, a safe and effective solution scheme derived from natural plants is provided for preventing premature ovarian failure, and the application prospect is wide. The method can be widely applied to preparation of various products.

The invention belongs to the technical field of biological medicines, and particularly relates to application of neophytadiene in preparation of a medicine for preventing and treating premature senility of lung cells. According to the invention, the biological character change, the apparent transcriptional regulation effect and the internal molecular mechanism of the premature senility of the lung cells induced by the nano polystyrene are studied. Wherein the exposure of the nano polystyrene can induce the increase of cell premature senility phenotype, SA-beta-gal activity, ROS and SASP levels, and S-phase retardation of a cell cycle, and can activate the methylation modification of m6A and m7G of lung cell RNA, up-regulate the expression of RNA methyltransferase, promote the combination of METTL1 and a premature senility core gene, and accelerate the premature senility of cells. The neophytadiene NPT intervention can remarkably reverse ROS and SASP levels in cells, effectively relieve the cell cycle arrest degree, reverse the expression abnormity of RNA methylation regulatory enzyme, delay the premature senility process and the like. The invention provides a new thought and direction for prevention and intervention measures of nano-plastic induced lung injury.

The invention belongs to the field of biological medicine, and relates to a preparation method of a premature senility animal model. The invention provides an RNA (Ribonucleic Acid), wherein the RNA is selected from any sequence shown in SEQ ID NO: 1-3. The RNA is sgRNA, the sgRNA can specifically target the Lmna c.1827 site, and the Lmna c.1827 site is edited by combining a base editing protein, so that the premature senility animal model is successfully obtained. And a powerful model animal model tool is provided for deeply researching the pathogenesis of the premature senility and finding a potential treatment method.

Leaf development, especially in leafy greens, is crucial for crop productivity. In various plants, DA1, a pivotal ubiquitin receptor that negatively regulates leaf growth, and EOD1 (ENHANCER1 OF DA1), an E3 ubiquitin ligases that interacts with DA1 through its RING domain, influence organ size. In this disclosure, in lettuce (Lactuca sativa), homologs genes were identified for both DA1 and EOD1, and da1 single mutants, da1 / eod1 double mutants, and DA1 overexpressing lines (DA1ox) were created. The da1 / eod1 double mutant exhibited a striking phenotype with larger organ size, accelerated growth, and increased biomass, resulting in higher yields and rapid growth traits pivotal for Controlled Environment Agriculture (CEA). Additionally, the da1 / eod1 double mutant displayed a pronounced ‘stay-green’ phenotype, characterized by elevated chlorophyll b levels, which implies extended shelf life, and enhanced postharvest quality, both traits indispensable for leafy vegetables in CEA settings. In contrast, the DA1ox line exhibited early senescence and diminished organ size. Based on these findings that spotlight the nuanced roles of the DA1-EOD1 module in modulating plant growth phases and its potential to revolutionize breeding strategies for improved yield and postharvest longevity in CEA-cultivated leafy greens, this disclosure provides guide RNAs for gene editing of DA1 and EOD1, and transformed lettuce having improved organ size and postharvest quality.

The invention discloses a corn leaf aging control gene ZmPLS1 and application thereof. According to the invention, firstly, a ZmPLS1 natural mutant zmpls1 with premature senility leaves is identified in breeding improved offspring of a corn excellent inbred line Jing 724, and the photosynthetic pigment content of the ZmPLS1 natural mutant zmpls1 is found to be obviously reduced. In order to further verify the function of the ZmPLS1, the invention also constructs a ZmPLS1 knockout corn strain and a ZmPLS1 interference corn strain, and also discovers that the leaves of the ZmPLS1 knockout corn strain and the ZmPLS1 interference corn strain also have premature aging phenotypes and the content of photosynthetic pigments is significantly reduced. The ZmPLS1 protein participates in regulating and controlling the photosynthetic pigment content and the aging process of the corn leaves for the first time. The invention not only has important significance for clarifying the molecular mechanism of corn leaf senescence, but also has potential application value for delaying the senescence process of corn leaves, increasing the yield and improving the quality.

The invention relates to an application of light waves in preparation of a medical instrument for treating ovarian aging. The medical instrument generates light waves with energy of 2-50J / cm < 2 > and wavelength of 650nm + / -10nm. The propagation direction of guided light waves in vitro faces the ovary of a tested animal, and verification shows that the activity, proliferation and hormone secretion of KGN cells are remarkably protected, meanwhile, H2O2-induced KGN cell apoptosis is inhibited, the unbalance condition of pro-inflammatory and anti-inflammatory cytokines in an aged mouse is recovered, the mitochondrial function of the ovary of the aged mouse is improved, and the curative effect of the ovary of the aged mouse is improved. The characteristics of reproduction, endocrine and the like of naturally aged ovaries are effectively improved, and the ovarian function of reproductive aging (such as premature ovarian failure) can be recovered.

The application provides a molecular marker for detecting a premature senescence gene of crops and application thereof, and belongs to the technical field of crop breeding. The molecular marker for detecting the premature senescence gene of crops can detect the deletion of four bases AACA in the 11th exon of the coding region of the gene LOC_Os03g31550, so as to form a premature senescence related gene caused by frame shift mutation. Research shows that the rice containing the premature senescence related gene has a stable premature senescence phenotype, the leaf tips of the second leaf and the third leaf are dried and the premature senescence phenotype appears on the 10th day after sowing in a field environment, and the aging lasts to the mature period. The photosynthetic pigment content of mesophyll cells is obviously reduced in the tillering period, the chloroplast structure is changed, the light damage is serious, the photosynthesis is weakened, and the uric acid content is reduced. The application provides a molecular basis for further in-depth research on gene regulation of aging, and provides a reference for cultivating new varieties resistant to premature senescence by using the premature senescence gene.