1018 results about "Immune regulation" patented technology

The invention relates to an orthopedic postoperative drainage liquid infection risk identification method. Drainage liquid is regarded as system output jointly driven by postoperative local immune regulation and wound recovery; capturing a nonlinear time sequence curve; first-order derived trend modeling is carried out; abrupt change after the change rate is stable is analyzed; establishing a postoperative recovery normal path framework, and forming a standard recovery trend library by using drainage fluid dynamic parameters of non-infected cases; comparing the Euclidean offset degree and directional change between the drainage parameter behavior vector of the current patient and the standard trajectory; the change is converted into a visual recovery offset map, and infection prediction data is measured from a multi-day trend angle; constructing and identifying local abnormal signal aggregation; forming a framework of infection event transition to obtain clinical infection nodes of qualitative change caused by quantitative change; according to the method, early-stage accurate recognition can be realized, the intervention opportunity can be obviously advanced, single threshold misjudgment is avoided, the recognition accuracy and specificity are improved, the model adaptability is improved, and the recognition interpretability is enhanced.

The invention discloses bifidobacterium breve BBr69 with an immunoregulation function as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium breve BBr69 with the immunoregulation function is CGMCC (China General Microbiological Culture Collection Center) NO. 32425. Based on the strain, the invention also provides application and related products of the strain. The BBr69 strain has broad-spectrum antibacterial performance, has the function of regulating intestinal flora and also has the function of enhancing immunity, and immunity is enhanced by proliferating RAW264.7 cells, increasing the content of cell factors, inhibiting weight loss, increasing the index of immune organs and reducing ileum tissue damage.

The invention discloses a bran coat polypeptide GR-12 with an immunoregulation function and an application of the bran coat polypeptide GR-12, and the amino acid sequence of the bran coat polypeptide is Gly-Gln-Pro-Trp-Pro-Pro-Ala-Ser-Phe-Ala-Cys-Arg (GQPWPPASFACR, which is called GR-12 for short). Experiments find that the GR-12 can significantly enhance the proliferation activity and phagocytic activity of macrophages RAW264.7 and promote the macrophages RAW264.7 to secrete cytokines; meanwhile, it is found that the GR-12 can significantly enhance the immunomodulatory function of an immunosuppressive mouse induced by cyclophosphamide, specifically, the body weight, thymus index and spleen index of the immunosuppressive mouse are significantly recovered; and the secretion of cytokines and immune globulins in mouse serum is obviously increased. The bran coat polypeptide GR-12 is expected to be further researched and developed into a related medicine product with an immune regulation function, and has a wide application prospect.

The invention discloses an immunoregulation hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials, amino-derived cationic polysaccharide, carboxyl-derived anionic polysaccharide and dopa-modified protein biopolymer materials are used as biological matrix materials, spermidine is used as a bioactive functional component, and the immunoregulation hydrogel is prepared by a one-step method. The preparation method comprises the following steps: by taking a benzaldehyde-terminated high polymer material as a cross-linking agent, forming a first layer of dynamic reversible cross-linked network structure through Schiff base reaction; then calcium ions are introduced, and the calcium ions and carboxylate radicals are complexed and cross-linked to form a second layer of static irreversible cross-linked network structure which can be used for an ESD (Electro-Static Discharge) operation. By adopting a step-by-step cross-linking mode, the first-step cross-linked single-network hydrogel has injectability, self-repairability, tissue adhesiveness and an immune regulation function, and the second-step cross-linking further ensures stable and lasting performance, so that the problems that a submucosal injection material is easy to disperse, short in lasting time and poor in stability are solved. The immunoregulation function effect of the wound repair material is poor.

The invention discloses pediococcus acidilactici and application thereof, and relates to the technical field of microorganisms. The pediococcus acidilactici is pediococcus acidilactici Mei.1103.1, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.32564, the preservation date is November 11, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1 is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1. The invention also provides application of the fermented dry rice flour in preparation of products for reducing uric acid, preventing / treating hyperuricemia, regulating immunity, preventing / treating kidney injury and preventing / treating fatty liver and fermented dry rice flour. The bacterium can reduce uric acid and relieve hyperuricemia; regulating blood fat; kidney and liver injuries caused by hyperuricemia are repaired; the content of volatile flavor compounds in the fermented dry rice flour is increased.

The invention belongs to the field of microorganisms. The invention provides a compound strain, a microbial inoculum and application of the compound strain in preparation of fermented East purple perilla tea. The compound strain comprises phytobacterium plantarum 2HDS3-La5 with the preservation number of CGMCC NO.34339, pediococcus acidilactici 2HDS3-SM6 with the preservation number of CGMCC NO.34337, Witzmania clostridium N2-La1 with the preservation number of CGMCC NO.34338, and the microbial inoculum is applied to preparation of fermented East purple perilla tea. The invention also provides application of the compound strain or a microbial inoculum containing the compound strain in preparation of fermented East purple perilla tea. The compound strain disclosed by the invention is applied to preparation of the fermented Dongbao tea, the content of substances with anti-inflammatory, anti-oxidation and immunoregulation effects in the Dongbao tea is remarkably increased by multiple times, and the prepared fermented Dongbao tea can remarkably improve the cell activity.

The present disclosure belongs to the technical fields of computational medicine and clinical oncology, and specifically relates to a molecular subtyping method for gastric cancer (GC) based on metabolic genes, a construction method for a prognostic prediction model and applications. In the present disclosure, metabolic process alterations are overall assessed, and new metabolic transcription patterns based on 456 metabolic gene-related signatures are identified. Three different metabolic patterns are validated by unsupervised consensus clustering analysis and independent datasets, and molecular and clinical pathological features of these three metabolite clusters have different metabolic gene expressions, pathway enrichments, gene variations, and survival outcomes. In addition, in the present disclosure, a scoring scheme is constructed to assess metabolic patterns of individual tumors, revealing the relationships between the metabolic patterns with prognosis, cuproptosis and immune regulation, and having a good value for practical application.

The invention relates to paclitaxel ganoderma lucidum spore oil self-nanoemulsion composite particles with ganoderma lucidum spores as a carrier and a preparation method of the paclitaxel ganoderma lucidum spore oil self-nanoemulsion composite particles, and belongs to the technical field of medicines. Pretreated Ganoderma lucidum spores (GLS) are obtained through process screening and serve as a drug carrier, and paclitaxel-loaded Ganoderma lucidum spore oil self-nanoemulsion (PGS) is loaded into the Ganoderma lucidum spores (GLS) to obtain paclitaxel-loaded composite particles (PGS (at) GLS). An in-vitro release curve shows that the PGS-atGLS has the characteristic of slow release after reaching the intestinal tract; in-vivo pharmacodynamic experiments show that PGS-atGLS can significantly inhibit development and metastasis of mouse colorectal cancer; tumor tissue slice immunofluorescence analysis shows that PGS-atGLS can effectively play a role in treating colon cancer. The paclitaxel self-nanoemulsion-loaded ganoderma lucidum spore composite particles synergistically promote cancer immune circulation by inducing immunogen cell death and immunoregulation, significantly improve the treatment effect of colon cancer, and have good biological safety.

The invention belongs to the cross technical field of nanomedicine, tumor treatment and immunotherapy, and particularly provides tumor mitochondria-targeted copper death selective induction nanoparticles, which are prepared by the following preparation method: S1, specifically coupling TPY and MHI through condensation reaction to obtain MTPY; s2, MTPY and copper ions are subjected to complexation, and an MTPY-Cu complex is obtained; and S3, the MTPY-Cu complex and albumin are subjected to self-assembly, and the MTPY-Cu-coated Alb nanoparticles are formed. The dosage of copper is only 1 / 1000 of that of free Cu < 2 + >, and equivalent immune regulation and killing effects can be achieved; primary tumors and distant tumor focuses can be inhibited, and lung metastasis is reduced; immune memory can be induced, and relapse can be prevented; the traditional Chinese medicine composition is combined with cis-platinum to remarkably enhance the curative effect and reverse the induced immunosuppression; the invention has the advantages of high biological safety and no obvious liver and kidney toxicity or hemolytic reaction.

The invention relates to the field of medicinal chemistry, and particularly discloses a piperidine derivative as well as a pharmaceutical composition, application and a preparation method thereof. The piperidine derivative has the following general formula. The piperidine derivative can be effectively used for treating or preventing multiple sclerosis, and shows an unexpected synergistic effect in an animal model: the piperidine derivative not only can significantly improve neurological function impairment and promote recovery, but also can deeply reduce the levels of key proinflammatory cytokines IFN-gamma and IL-17 at the same time. The invention provides a novel small molecule candidate drug with central permeability, neuroprotection and comprehensive immune regulation functions for treatment of multiple sclerosis.

The invention discloses a preparation method and application of a sea cucumber exosome with an immune cell repairing function, and belongs to the technical field of biomedicine. The method comprises the following steps: feeding sea cucumbers by using a combined method of composite stress and an echinacea extract, extracting sea cucumber cells, and obtaining sea cucumber exosomes through ultracentrifugation; wherein the condition of composite stress is set as follows: the day and night temperature difference is not lower than 3 DEG C, and meanwhile, the dissolved oxygen content of the water body is adjusted to 75-90% of the dissolved oxygen content of the conventional feeding water body. The obtained sea cucumber exosome has a remarkable immune cell repairing function, and can effectively promote proliferation and activity recovery of immune cells, inhibit expression of inflammatory factors and improve the level of anti-inflammatory factors. The sea cucumber exosome prepared by the preparation method disclosed by the invention has an important application prospect in the research and development of immune repair drugs, and can provide a technical support for a novel immune regulation preparation in the field of biomedicine.

The invention relates to preparation of a medicinal and edible active ingredient composition with an immune regulation function. According to the composition, main effective components in medicinal and edible plants including fructus arctii and codonopsis pilosula are adopted, and arctigenin and codonopsis pilosula polysaccharide are combined, so that an immune regulation function is realized. Each experimental index of the composition is superior to that of the single action of the effective components, and the comprehensive effect of the synergistic effect is embodied. The composition is derived from effective components of traditional Chinese medicines with homology of medicine and food, can be applied to susceptible people with immune imbalance, can improve inflammatory response of swollen sore throat and the like, and has wide health-care function development and application prospects.

Embodiments of the invention include compositions and methods related to engineered human mucosal-associated invariant T (eMAIT) cells for off-the-shelf use for clinical therapy for cancer, infectious, and autoimmune diseases. In some embodiments, the eMAIT cells are produced from healthy human donor peripheral blood, cord blood, or G-CSF mobilized peripheral blood. In particular embodiments, the eMAIT cells are produced from a pluripotent stem cell line and therefore can be of unlimited supply. In some embodiments, the eMAIT cells are engineered to express chimeric antigen receptors (CARs), or / and immune regulatory molecules, or / and allorejection resistance molecules. Embodiments of the invention also include compositions of matter comprising polynucleotides encoding mucosal-associated invariant T cell receptor alpha chain polypeptides and / or mucosal-associated invariant T cell receptor beta chain polypeptides.

A material for producing macromolecule air fibers, a preparation method, and an application thereof are provided. The material for producing macromolecule air fiber is prepared by preparation of doped antibacterial TiO2 / ZnO / ZrO2 ceramic powder, deposition of magnetic iron oxide, surface modification with tannic acid, addition of water-soluble chitosan solution, immersion in a spined TPEE air fiber membrane, uniform addition of Genipin solution, heating and reacting, membrane removal, washing, and drying. The TPEE air fiber membrane produced by the present disclosure has good mechanical properties, and modified ceramic powder is uniformly dispersed in the TPEE air fiber membrane, which plays a good role in antibacterial, environmental protection, immune regulation, magnetic therapy, far-infrared heating and other effects, rendering the material for producing macromolecule air fibers have better comprehensive performance and a good mattress support material with broad application prospects.

The invention discloses a method for identifying key mitochondrial autophagy and ferroptosis genes in sepsis-induced ARDS and an immunoregulation mechanism of the key mitochondrial autophagy and ferroptosis genes. The method comprises the following steps: screening differential expression genes DEGs related to the sepsis-induced ARDS from a GEO gene expression database; and screening the DEGs by using LASSO and SVM + RFE, and identifying 12 key DEGs. The diagnostic potential of the genes in sepsis-induced ARDS is verified through an ROC curve, and the effect of the genes in immune response is disclosed through gene enrichment analysis and immune infiltration analysis. According to the invention, a key gene FTH1 is identified, a miRNA gene [miR-224-5p] targeting the FTH1 and a pathologic process in which the miRNA gene [miR-224-5p] participates in regulation and control of sepsis-induced ARDS are found, and a close relationship between the FTH1 and E3 ubiquitin ligase SMURF1 is found.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention provides a fermented feed formula for enhancing animal immune function and a preparation process thereof, and belongs to the field of fermented feed. The preparation process comprises the following steps: preparing the tortoise shell extract; preparing a plant extract; and preparing the fermented feed. The tortoise shell extract and the acacia farnesiana extract are prepared respectively and then added into the fermentation base material to prepare the fermented feed, so that the tortoise shell extract can provide a material basis for proliferation of immune cells and synthesis of immunocompetence substances; the tortoise shell extract creates a good internal environment for normal function exertion of an immune system, in addition, microelements in the tortoise shell extract are matched with bioactive components in the acacia extract, uptake and utilization of the microelements by immune cells are further enhanced, so that the immune regulation effect is more effectively exerted, and the immunity of the human body is improved. The effect of synergistically enhancing the animal immune function is achieved.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention belongs to the technical field of biological medicines, discloses application of bifidobacterium breve BB990 in the field of metabolism, and particularly discloses bifidobacterium breve BB990 which is preserved in Guangdong Microorganism Culture Collection Center, the preservation number is GDMCC No.63259, and the preservation date is March 10, 2023. The invention further discloses a preparation method of the bifidobacterium breve BB990. The bifidobacterium breve BB990 provided by the invention has the effects of regulating gastrointestinal disorder, enhancing intestinal immune function, inhibiting harmful bacteria, improving intestinal flora structure, maintaining intestinal steady state, regulating immunity, improving immune curative effect, regulating body glycometabolism and liver metabolism and the like. Experiments prove that the bifidobacterium breve BB990 can effectively prevent and treat metabolic diseases, such as non-alcoholic fatty liver, type II diabetes and the like, the treatment effect of the bifidobacterium breve BB990 is remarkably superior to that of other bifidobacterium breve (such as bifidobacterium breve 206), and the bifidobacterium breve BB990 has a good prospect.

The invention belongs to the technical field of biological medicines, and relates to application of Ackermann muciniphile or its external vesicles in preparation of a drug for treating schistosomiasis. The anti-infection immunity of a host is enhanced by adjusting intestinal flora balance (recovering diversity and composition) and improving immune response (adjusting macrophage, CD8 + T cell recruitment and Th1 / Th2 balance); meanwhile, the intestinal barrier function is improved, and pathogen invasion and inflammation are reduced; hepatic granuloma and hepatic fibrosis caused by schistosome infection are obviously relieved, and the liver function is improved; the key effect of the MIF gene in immunoregulation is disclosed, and a new direction is provided for schistosomiasis immunotherapy. Besides, the probiotics are high in treatment safety and free of obvious side effects, can be combined with chemotherapy to enhance the curative effect and reduce the side effects and drug resistance of chemical drugs, provides a lasting and comprehensive adjuvant therapy scheme for the schistosomiasis, and has important clinical application potential.

The invention belongs to the technical field of active peptide preparation, and particularly relates to an active collagen peptide with an immunoregulation function and a preparation method and application thereof. The active collagen peptide with an immunoregulation function is prepared by using a method of performing composite enzymolysis and separating and collecting specified collection liquid by using a chromatographic column, and a new thought is provided for improving the immunity; the prepared active collagen peptide can significantly improve the proliferative activity, phagocytic ability and NO secretion amount of RAW264.7 cells and change the cell morphology, has an important regulation effect on immune balance recovery of immunosuppression model mice, and can regulate the problem of low immunologic function of mice induced by cyclophosphamide, regulate immune balance and improve the body immunity.

The invention particularly relates to a hydrogel drug delivery system capable of releasing drugs in a time sequence and a preparation method of the hydrogel drug delivery system, and aims to meet the requirements of different stages of wound repair and realize optimization of anti-inflammatory and osteogenesis effects. The invention relates to a hydrogel drug-loading system capable of releasing drugs in time sequence. The hydrogel drug-loading system comprises hydrogel, a first drug and drug-loading microspheres, the material of the hydrogel comprises tannic acid modified silk fibroin; the first medicine and the medicine carrying microspheres are dispersed in the hydrogel; the first medicine is at least used for resisting inflammation; the drug-loaded microsphere comprises a second drug and a shell layer surrounding the second drug; the second medicine is at least used for promoting osteogenesis. According to the present invention, the wound can be completely closed in the early stage of tooth extraction wound healing so as to achieve the bleeding stopping purpose, the beneficial immunoregulation is performed to achieve the anti-inflammation effect, the beneficial osteogenesis microenvironment can be created in the later stage of tooth extraction wound healing, and the drug carrier material can be used in the technical field of the drug carrier material.

The invention belongs to the technical field of bone repair hydrogel, and discloses a hydrogel band-aid for bone repair and a preparation method and application thereof, and the preparation method comprises the following steps: mixing a polyethylene glycol succinimide succinate solution with an alendronate solution, dialyzing, and freeze-drying to obtain alendronate modified polyethylene glycol succinimide succinate; and uniformly mixing alendronate modified polyethylene glycol succinimide succinate, polyphenol, metal ions and water in proportion, carrying out solid-liquid separation, and drying the solid to obtain the hydrogel. The preparation process provided by the invention has the advantages of simplicity, convenience and rapidness, not only simplifies the production process, but also enhances the bone regeneration, angiogenesis and immunoregulation capabilities.

The invention relates to streptococcus thermophilus CT-02 with an immune regulation effect and application of the streptococcus thermophilus CT-02, the classification name of the streptococcus thermophilus CT-02 with the immune regulation effect is Streptococcus thermophilus, the preservation number of the streptococcus thermophilus CT-02 is GDMCC No: 64474, and the preservation date of the streptococcus thermophilus CT-02 is March 29, 2024. The strain has an excellent immune regulation effect which is specifically shown as follows: by moderately activating macrophages, not only can the excessive activation of TNF-alpha, IL-1beta, IL-6 and IL-12 be avoided, but also the activation level of IL-10 can be improved, that is, the levels of various inflammatory factors secreted by the macrophages are comprehensively regulated, and the strain has a potential anti-inflammatory effect while activating immunity. The strain has a prospect for preparing a product with an immune regulation effect or a product with an inflammatory factor regulation effect.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a function-enhanced mesenchymal stem cell exosome, the preparation method comprises the following steps: S1, preparing an NK cell exosome, collecting supernate in an NK cell culture medium, and treating the supernate by using an optimized exosome separation and purification process to obtain an NK cell exosome; finishing preparation of a stock solution; s2, culturing the mesenchymal stem cells, culturing the cells in a 3D suspension culture mode, treating and controlling low oxygen concentration by combining with NK cell exosomes, and simulating an in-vivo inflammation microenvironment in a multi-azimuth manner, so that the exosomes secreted by the mesenchymal stem cells have a stronger immune regulation function and an anti-inflammatory effect; s3, preparation of mesenchymal stem cell exosomes: preparing a stock solution through an exosome separation and purification process combining a tangential flow ultrafiltration technology and a PS affinity chromatography technology; the exosome stock solution obtained by the method is high in purity and high in yield, and has more excellent biological activity and excellent treatment effect.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The invention belongs to the technical field of biological medicine, and particularly relates to a stem cell preparation based on immunoregulation and a preparation method and application thereof. The stem cell preparation based on immunoregulation is prepared from the following raw materials in parts by weight: 5 to 40 parts of freeze-dried powder, 40 to 120 parts of gastrodia elata polysaccharide and 2 to 6 parts of melatonin, the freeze-dried powder is obtained by mixing and freeze-drying a stem cell culture solution and curcumin lipidosome; the volume ratio of the stem cell culture solution to the curcumin liposome is 1: (10-32); the protein concentration in the stem cell culture solution is 1.2 mg / mL to 1.6 mg / mL. The stem cell preparation provided by the invention can utilize the immune regulation effect of the mesenchymal stem cells to realize the immune repair of adipose tissues.

A composition includes postbiotics comprising two or more of the following components: Lactobacillus acidophilus, Lactobacillus rhamnosus, and Saccharomyces boulardii; wherein the postbiotics are dead, whole-cell postbiotics. A method of providing to a subject an immunomodulatory, immune regulatory and / or immune boosting effect through administering the composition is also provided. A method of making the postbiotic composition is also provided.

The invention belongs to the technical field of medicines, and provides a novel method for overcoming multidrug resistance of tumors based on innate immune regulation. The invention relates to an application of an agonist of an innate immune STING pathway and an ENPP1 inhibitor in overcoming multidrug resistance of tumors and enhancing an anti-tumor curative effect. The STING agonist or the ENPP1 inhibitor is combined with an anti-tumor chemical drug for application, so that the effects of overcoming the multi-drug resistance of chemotherapeutic drugs and enhancing the anti-tumor curative effect are achieved. The STING agonist / or ENPP1 inhibitor has the effect of reversing multidrug resistance of tumors, and can enhance the sensitivity of cancer cells to chemotherapeutic drugs and monoclonal antibody drugs, so that the curative effect of antitumor drugs is enhanced, and the STING agonist / or ENPP1 inhibitor is expected to be developed into a novel reversal agent drug for multidrug resistance tumors. The novel anti-multidrug resistance strategy provides a new thought for drug design and cancer treatment, and has a great application prospect in clinical treatment of cancers.

The invention discloses a microbial compound feed for improving disease resistance of blue crabs and a preparation method of the microbial compound feed, and belongs to the technical field of feed preparation. The microbial compound feed comprises the following raw materials in parts by weight: 30-40 parts of fermented soybean meal, 20-25 parts of bran, 15-20 parts of fish meal, 5-8 parts of a compound microbial agent, 3-5 parts of algal polysaccharides, 2-3 parts of a radix astragali extract, 1-2 parts of chitosan, 2-3 parts of a mineral premix and 1-1.5 parts of compound vitamins, wherein the compound microbial agent comprises bacillus licheniformis, lactobacillus acidophilus and saccharomyces cerevisiae. The microbial compound feed disclosed by the invention comprehensively improves the disease resistance of the blue crabs and improves the survival rate through various ways, such as nutrition balance, immune regulation and intestinal health improvement, so that the breeding cost is reduced, and the income of farmers is increased.