825 results about "Immune regulation" patented technology

The invention relates to an orthopedic postoperative drainage liquid infection risk identification method. Drainage liquid is regarded as system output jointly driven by postoperative local immune regulation and wound recovery; capturing a nonlinear time sequence curve; first-order derived trend modeling is carried out; abrupt change after the change rate is stable is analyzed; establishing a postoperative recovery normal path framework, and forming a standard recovery trend library by using drainage fluid dynamic parameters of non-infected cases; comparing the Euclidean offset degree and directional change between the drainage parameter behavior vector of the current patient and the standard trajectory; the change is converted into a visual recovery offset map, and infection prediction data is measured from a multi-day trend angle; constructing and identifying local abnormal signal aggregation; forming a framework of infection event transition to obtain clinical infection nodes of qualitative change caused by quantitative change; according to the method, early-stage accurate recognition can be realized, the intervention opportunity can be obviously advanced, single threshold misjudgment is avoided, the recognition accuracy and specificity are improved, the model adaptability is improved, and the recognition interpretability is enhanced.

The invention discloses a bran coat polypeptide GR-12 with an immunoregulation function and an application of the bran coat polypeptide GR-12, and the amino acid sequence of the bran coat polypeptide is Gly-Gln-Pro-Trp-Pro-Pro-Ala-Ser-Phe-Ala-Cys-Arg (GQPWPPASFACR, which is called GR-12 for short). Experiments find that the GR-12 can significantly enhance the proliferation activity and phagocytic activity of macrophages RAW264.7 and promote the macrophages RAW264.7 to secrete cytokines; meanwhile, it is found that the GR-12 can significantly enhance the immunomodulatory function of an immunosuppressive mouse induced by cyclophosphamide, specifically, the body weight, thymus index and spleen index of the immunosuppressive mouse are significantly recovered; and the secretion of cytokines and immune globulins in mouse serum is obviously increased. The bran coat polypeptide GR-12 is expected to be further researched and developed into a related medicine product with an immune regulation function, and has a wide application prospect.

The invention discloses an immunoregulation hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials, amino-derived cationic polysaccharide, carboxyl-derived anionic polysaccharide and dopa-modified protein biopolymer materials are used as biological matrix materials, spermidine is used as a bioactive functional component, and the immunoregulation hydrogel is prepared by a one-step method. The preparation method comprises the following steps: by taking a benzaldehyde-terminated high polymer material as a cross-linking agent, forming a first layer of dynamic reversible cross-linked network structure through Schiff base reaction; then calcium ions are introduced, and the calcium ions and carboxylate radicals are complexed and cross-linked to form a second layer of static irreversible cross-linked network structure which can be used for an ESD (Electro-Static Discharge) operation. By adopting a step-by-step cross-linking mode, the first-step cross-linked single-network hydrogel has injectability, self-repairability, tissue adhesiveness and an immune regulation function, and the second-step cross-linking further ensures stable and lasting performance, so that the problems that a submucosal injection material is easy to disperse, short in lasting time and poor in stability are solved. The immunoregulation function effect of the wound repair material is poor.

The invention discloses pediococcus acidilactici and application thereof, and relates to the technical field of microorganisms. The pediococcus acidilactici is pediococcus acidilactici Mei.1103.1, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.32564, the preservation date is November 11, 2024, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1 is CGMCC NO.32564. The pediococcus acidilactici Mei.1103.1. The invention also provides application of the fermented dry rice flour in preparation of products for reducing uric acid, preventing / treating hyperuricemia, regulating immunity, preventing / treating kidney injury and preventing / treating fatty liver and fermented dry rice flour. The bacterium can reduce uric acid and relieve hyperuricemia; regulating blood fat; kidney and liver injuries caused by hyperuricemia are repaired; the content of volatile flavor compounds in the fermented dry rice flour is increased.

The invention belongs to the field of microorganisms. The invention provides a compound strain, a microbial inoculum and application of the compound strain in preparation of fermented East purple perilla tea. The compound strain comprises phytobacterium plantarum 2HDS3-La5 with the preservation number of CGMCC NO.34339, pediococcus acidilactici 2HDS3-SM6 with the preservation number of CGMCC NO.34337, Witzmania clostridium N2-La1 with the preservation number of CGMCC NO.34338, and the microbial inoculum is applied to preparation of fermented East purple perilla tea. The invention also provides application of the compound strain or a microbial inoculum containing the compound strain in preparation of fermented East purple perilla tea. The compound strain disclosed by the invention is applied to preparation of the fermented Dongbao tea, the content of substances with anti-inflammatory, anti-oxidation and immunoregulation effects in the Dongbao tea is remarkably increased by multiple times, and the prepared fermented Dongbao tea can remarkably improve the cell activity.

The invention belongs to the cross technical field of nanomedicine, tumor treatment and immunotherapy, and particularly provides tumor mitochondria-targeted copper death selective induction nanoparticles, which are prepared by the following preparation method: S1, specifically coupling TPY and MHI through condensation reaction to obtain MTPY; s2, MTPY and copper ions are subjected to complexation, and an MTPY-Cu complex is obtained; and S3, the MTPY-Cu complex and albumin are subjected to self-assembly, and the MTPY-Cu-coated Alb nanoparticles are formed. The dosage of copper is only 1 / 1000 of that of free Cu < 2 + >, and equivalent immune regulation and killing effects can be achieved; primary tumors and distant tumor focuses can be inhibited, and lung metastasis is reduced; immune memory can be induced, and relapse can be prevented; the traditional Chinese medicine composition is combined with cis-platinum to remarkably enhance the curative effect and reverse the induced immunosuppression; the invention has the advantages of high biological safety and no obvious liver and kidney toxicity or hemolytic reaction.

The invention relates to the field of medicinal chemistry, and particularly discloses a piperidine derivative as well as a pharmaceutical composition, application and a preparation method thereof. The piperidine derivative has the following general formula. The piperidine derivative can be effectively used for treating or preventing multiple sclerosis, and shows an unexpected synergistic effect in an animal model: the piperidine derivative not only can significantly improve neurological function impairment and promote recovery, but also can deeply reduce the levels of key proinflammatory cytokines IFN-gamma and IL-17 at the same time. The invention provides a novel small molecule candidate drug with central permeability, neuroprotection and comprehensive immune regulation functions for treatment of multiple sclerosis.

The invention discloses a preparation method and application of a sea cucumber exosome with an immune cell repairing function, and belongs to the technical field of biomedicine. The method comprises the following steps: feeding sea cucumbers by using a combined method of composite stress and an echinacea extract, extracting sea cucumber cells, and obtaining sea cucumber exosomes through ultracentrifugation; wherein the condition of composite stress is set as follows: the day and night temperature difference is not lower than 3 DEG C, and meanwhile, the dissolved oxygen content of the water body is adjusted to 75-90% of the dissolved oxygen content of the conventional feeding water body. The obtained sea cucumber exosome has a remarkable immune cell repairing function, and can effectively promote proliferation and activity recovery of immune cells, inhibit expression of inflammatory factors and improve the level of anti-inflammatory factors. The sea cucumber exosome prepared by the preparation method disclosed by the invention has an important application prospect in the research and development of immune repair drugs, and can provide a technical support for a novel immune regulation preparation in the field of biomedicine.

Embodiments of the invention include compositions and methods related to engineered human mucosal-associated invariant T (eMAIT) cells for off-the-shelf use for clinical therapy for cancer, infectious, and autoimmune diseases. In some embodiments, the eMAIT cells are produced from healthy human donor peripheral blood, cord blood, or G-CSF mobilized peripheral blood. In particular embodiments, the eMAIT cells are produced from a pluripotent stem cell line and therefore can be of unlimited supply. In some embodiments, the eMAIT cells are engineered to express chimeric antigen receptors (CARs), or / and immune regulatory molecules, or / and allorejection resistance molecules. Embodiments of the invention also include compositions of matter comprising polynucleotides encoding mucosal-associated invariant T cell receptor alpha chain polypeptides and / or mucosal-associated invariant T cell receptor beta chain polypeptides.

A material for producing macromolecule air fibers, a preparation method, and an application thereof are provided. The material for producing macromolecule air fiber is prepared by preparation of doped antibacterial TiO2 / ZnO / ZrO2 ceramic powder, deposition of magnetic iron oxide, surface modification with tannic acid, addition of water-soluble chitosan solution, immersion in a spined TPEE air fiber membrane, uniform addition of Genipin solution, heating and reacting, membrane removal, washing, and drying. The TPEE air fiber membrane produced by the present disclosure has good mechanical properties, and modified ceramic powder is uniformly dispersed in the TPEE air fiber membrane, which plays a good role in antibacterial, environmental protection, immune regulation, magnetic therapy, far-infrared heating and other effects, rendering the material for producing macromolecule air fibers have better comprehensive performance and a good mattress support material with broad application prospects.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention provides a fermented feed formula for enhancing animal immune function and a preparation process thereof, and belongs to the field of fermented feed. The preparation process comprises the following steps: preparing the tortoise shell extract; preparing a plant extract; and preparing the fermented feed. The tortoise shell extract and the acacia farnesiana extract are prepared respectively and then added into the fermentation base material to prepare the fermented feed, so that the tortoise shell extract can provide a material basis for proliferation of immune cells and synthesis of immunocompetence substances; the tortoise shell extract creates a good internal environment for normal function exertion of an immune system, in addition, microelements in the tortoise shell extract are matched with bioactive components in the acacia extract, uptake and utilization of the microelements by immune cells are further enhanced, so that the immune regulation effect is more effectively exerted, and the immunity of the human body is improved. The effect of synergistically enhancing the animal immune function is achieved.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention belongs to the technical field of biological medicines, and relates to application of Ackermann muciniphile or its external vesicles in preparation of a drug for treating schistosomiasis. The anti-infection immunity of a host is enhanced by adjusting intestinal flora balance (recovering diversity and composition) and improving immune response (adjusting macrophage, CD8 + T cell recruitment and Th1 / Th2 balance); meanwhile, the intestinal barrier function is improved, and pathogen invasion and inflammation are reduced; hepatic granuloma and hepatic fibrosis caused by schistosome infection are obviously relieved, and the liver function is improved; the key effect of the MIF gene in immunoregulation is disclosed, and a new direction is provided for schistosomiasis immunotherapy. Besides, the probiotics are high in treatment safety and free of obvious side effects, can be combined with chemotherapy to enhance the curative effect and reduce the side effects and drug resistance of chemical drugs, provides a lasting and comprehensive adjuvant therapy scheme for the schistosomiasis, and has important clinical application potential.

The invention belongs to the technical field of active peptide preparation, and particularly relates to an active collagen peptide with an immunoregulation function and a preparation method and application thereof. The active collagen peptide with an immunoregulation function is prepared by using a method of performing composite enzymolysis and separating and collecting specified collection liquid by using a chromatographic column, and a new thought is provided for improving the immunity; the prepared active collagen peptide can significantly improve the proliferative activity, phagocytic ability and NO secretion amount of RAW264.7 cells and change the cell morphology, has an important regulation effect on immune balance recovery of immunosuppression model mice, and can regulate the problem of low immunologic function of mice induced by cyclophosphamide, regulate immune balance and improve the body immunity.

The invention relates to streptococcus thermophilus CT-02 with an immune regulation effect and application of the streptococcus thermophilus CT-02, the classification name of the streptococcus thermophilus CT-02 with the immune regulation effect is Streptococcus thermophilus, the preservation number of the streptococcus thermophilus CT-02 is GDMCC No: 64474, and the preservation date of the streptococcus thermophilus CT-02 is March 29, 2024. The strain has an excellent immune regulation effect which is specifically shown as follows: by moderately activating macrophages, not only can the excessive activation of TNF-alpha, IL-1beta, IL-6 and IL-12 be avoided, but also the activation level of IL-10 can be improved, that is, the levels of various inflammatory factors secreted by the macrophages are comprehensively regulated, and the strain has a potential anti-inflammatory effect while activating immunity. The strain has a prospect for preparing a product with an immune regulation effect or a product with an inflammatory factor regulation effect.

The invention relates to the technical field of biological medicine, in particular to a preparation method and application of a function-enhanced mesenchymal stem cell exosome, the preparation method comprises the following steps: S1, preparing an NK cell exosome, collecting supernate in an NK cell culture medium, and treating the supernate by using an optimized exosome separation and purification process to obtain an NK cell exosome; finishing preparation of a stock solution; s2, culturing the mesenchymal stem cells, culturing the cells in a 3D suspension culture mode, treating and controlling low oxygen concentration by combining with NK cell exosomes, and simulating an in-vivo inflammation microenvironment in a multi-azimuth manner, so that the exosomes secreted by the mesenchymal stem cells have a stronger immune regulation function and an anti-inflammatory effect; s3, preparation of mesenchymal stem cell exosomes: preparing a stock solution through an exosome separation and purification process combining a tangential flow ultrafiltration technology and a PS affinity chromatography technology; the exosome stock solution obtained by the method is high in purity and high in yield, and has more excellent biological activity and excellent treatment effect.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

A composition includes postbiotics comprising two or more of the following components: Lactobacillus acidophilus, Lactobacillus rhamnosus, and Saccharomyces boulardii; wherein the postbiotics are dead, whole-cell postbiotics. A method of providing to a subject an immunomodulatory, immune regulatory and / or immune boosting effect through administering the composition is also provided. A method of making the postbiotic composition is also provided.

The invention belongs to the technical field of medicines, and provides a novel method for overcoming multidrug resistance of tumors based on innate immune regulation. The invention relates to an application of an agonist of an innate immune STING pathway and an ENPP1 inhibitor in overcoming multidrug resistance of tumors and enhancing an anti-tumor curative effect. The STING agonist or the ENPP1 inhibitor is combined with an anti-tumor chemical drug for application, so that the effects of overcoming the multi-drug resistance of chemotherapeutic drugs and enhancing the anti-tumor curative effect are achieved. The STING agonist / or ENPP1 inhibitor has the effect of reversing multidrug resistance of tumors, and can enhance the sensitivity of cancer cells to chemotherapeutic drugs and monoclonal antibody drugs, so that the curative effect of antitumor drugs is enhanced, and the STING agonist / or ENPP1 inhibitor is expected to be developed into a novel reversal agent drug for multidrug resistance tumors. The novel anti-multidrug resistance strategy provides a new thought for drug design and cancer treatment, and has a great application prospect in clinical treatment of cancers.

The invention discloses a microbial compound feed for improving disease resistance of blue crabs and a preparation method of the microbial compound feed, and belongs to the technical field of feed preparation. The microbial compound feed comprises the following raw materials in parts by weight: 30-40 parts of fermented soybean meal, 20-25 parts of bran, 15-20 parts of fish meal, 5-8 parts of a compound microbial agent, 3-5 parts of algal polysaccharides, 2-3 parts of a radix astragali extract, 1-2 parts of chitosan, 2-3 parts of a mineral premix and 1-1.5 parts of compound vitamins, wherein the compound microbial agent comprises bacillus licheniformis, lactobacillus acidophilus and saccharomyces cerevisiae. The microbial compound feed disclosed by the invention comprehensively improves the disease resistance of the blue crabs and improves the survival rate through various ways, such as nutrition balance, immune regulation and intestinal health improvement, so that the breeding cost is reduced, and the income of farmers is increased.

The invention relates to the technical field of biomedical engineering, in particular to engineered probiotics with a surface-modified protective shell, a preparation method and application of the engineered probiotics, the engineered probiotics with the surface-modified protective shell comprise the protective shell and engineered probiotics, and the engineered probiotics are coated with the protective shell; the engineered probiotics are subjected to surface modification through immune regulation factors, and metal ions are loaded on the surfaces of the engineered probiotics subjected to surface modification; the engineered probiotics with the surface modified protective shell can regulate immune factors, can be targeted and colonized in colon and promote repair of mucosa surface, and has a better treatment effect on enteritis, so that the engineered probiotics are expected to be applied to preparation of enteritis pharmaceutical preparations; according to the preparation method of the engineering probiotics, raw materials are easy to obtain, the operation process is simple and convenient, reaction conditions are controllable, and large-scale production is facilitated.

The invention belongs to the technical field of biological medicine, and particularly relates to a KGN-loaded nano vesicle, a soluble microneedle and a preparation method and application of the KGN-loaded nano vesicle and the soluble microneedle. The preparation method comprises the following steps: loading KGN into mesenchymal stem cell membrane nano vesicles by adopting a co-extrusion method to obtain KGN-atNVs; and then integrating the KGN-NVs into the dissolvable microneedle by adopting a micro-molding method to obtain the KGN-NVs-loaded dissolvable microneedle. The soluble microneedle loaded with the KGN-atNVs can effectively reduce expression of inflammatory factors such as IL-1beta and TNF-alpha, reduce joint inflammation, protect a cartilage structure, promote cartilage repair and improve joint functions. According to the KGNN-NV delivery system based on the dissolvable microneedle, the immune regulation and drug delivery advantages of the mesenchymal stem cell membrane nanovesicles and the percutaneous delivery characteristic of the dissolvable microneedle can be synergistically exerted, and an innovative and practical solution is provided for clinical treatment of arthritis.

The invention discloses injectable double-drug delivery hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. According to the method, two nano microspheres with different charges are respectively prepared by utilizing the characteristics of A-type gelatin and B-type gelatin, a negatively charged medicine EGCG is specifically carried by the positively charged A-type gelatin, and a positively charged medicine SAG is carried by the negatively charged B-type gelatin microsphere; and finally, mixing the two gelatin drug-loaded microspheres into a material taking gelatin and zirconium ions as a substrate to form the composite hydrogel. The hydrogel realizes immune regulation and osteogenesis regulation, also realizes long-acting release of the two drugs, effectively solves the problems of bone resorption, difficulty in healing and the like of host tissues through synergistic regulation of inflammation and mobilization of osteogenesis genes, realizes personalized treatment, and improves the clinical treatment effect and efficiency.

The invention discloses a method for promoting endothelialization of a heart valve and an artificial heart valve.The method is characterized in that circular DNA is modified on the surface of a decellularized valve (DHV), DNA hydrogel of targeted thrombin is obtained on the surface of the DHV through rolling circle amplification, the DNA hydrogel plays a role of a heme catcher while playing a role of the heme catcher, and heme is recruited from blood; heme is further converted into biliverdin (BV) through heme oxygenase (HO1) loaded in DNA, so that continuous production of biliverdin is ensured, anti-inflammatory immune regulation and active oxygen removal are promoted, a regenerative immune microenvironment is created, and endothelialization of the valve is promoted.

The invention discloses an effect of an Intestinia butyriproducens bacterium in anti-tumor immunotherapy, and finds that colonization of the Intestinia butyriproducens bacterium in intestinal tracts can inhibit growth of CT26 mouse subcutaneous transplanted tumors with poor response to the tumor immunotherapy, meanwhile, growth of human colorectal cancer cells is remarkably inhibited, and apoptosis of tumor cells is promoted for the first time. The application of the Intestinia butyriproducens bacterium in the anti-tumor immunotherapy has the advantages that the effect of the intestinia butyriproducens bacterium in the anti-tumor immunotherapy is achieved, and the application of the intestinia butyriproducens bacterium in the anti-tumor immunotherapy in the anti-tumor immunotherapy in the anti-tumor immunotherapy in the anti-tumor immunotherapy is achieved. Meanwhile, after colonization of the strain and combined administration of PD-1 monoclonal antibodies, the proportion of CD8 + T cells in mouse tumor infiltration lymphocytes is increased, the proportion of depleted PD-1 + CD8 + T cells is reduced, and the proportion of CD8 + T cells releasing functional cell factors interferon gamma and granzyme B is also increased, which shows that after colonization of the strain, the curative effect of tumor immunotherapy can be enhanced, and the tumor immunotherapy effect can be enhanced. The immunoregulation capability and the great clinical application value are realized.

The present invention discloses a chloroplast-sodium alginate microsphere and its preparation method and use. The preparation method of the chloroplast-sodium alginate microsphere comprises: mixing chloroplasts, sodium alginate and distilled water to obtain a mixed solution of chloroplasts and sodium alginate as a dispersed phase; dissolving a nonionic surfactant and CaI2 or CaCl2 in liquid paraffin to form an oil phase as a continuous phase; the dispersed phase and the continuous phase are cross-linked under the operation of a microfluidic device to form chloroplast-sodium alginate microspheres. The chloroplast-sodium alginate microspheres of the present invention have the ability to generate oxygen under illumination and are sustainable for up to two weeks. Chloroplasts are immunomodulated by reprogramming sugar metabolism to achieve anti-inflammatory and repair-promoting effects. The chloroplast-sodium alginate microspheres are suitable for wound repair, especially for chronic hypoxic wound repair similar to diabetic wounds.

The invention relates to the technical field of biological medicine, and discloses a culture medium for human metapneumovirus and application, the culture medium comprises the following components by mass: 850-920 parts of a basic culture medium; the metabolism reprogramming module is prepared from the following components in parts by weight: 0.05 to 0.15 part of 5-methyltetrahydrofolic acid, 0.3 to 0.7 part of N-acetyl-D-mannosamine and 1.0 to 3.0 parts of sodium dichloroacetate; the immune regulation and control module is prepared from 0.005 to 0.015 part of an RIG-I / MAVS pathway inhibitor; the receptor induction module is prepared from 0.5 to 1.5 parts of all-trans retinoic acid; the redox balance module is prepared from the following components in parts by weight: 0.1 to 0.5 part of pegylated superoxide dismutase and 0.002 to 0.008 part of sodium selenite. According to the method, the proportion of infectious virus particles can be remarkably increased, the generation of defective particles is reduced, meanwhile, the production cycle of host cells is prolonged, large-scale continuous culture is supported, and a high-quality and high-efficiency solution is provided for industrial production of gene therapy vectors and virus vaccines.

The invention relates to the field of functional food processing, and discloses a qi-tonifying and blood-activating dietary therapy formula based on the theory of qi and blood in traditional Chinese medicine, which comprises astragalus membranaceus, angelica sinensis, red dates, lycium barbarum, lycium ruthenicum, ginseng, salvia miltiorrhiza, mulberry, lucid ganoderma, cordyceps mycelium, rhodiola rosea, American ginseng, acanthopanax, grape seed extract and chrysanthemum. Through a compound formula based on the theory of qi and blood in traditional Chinese medicine, and by combining various core medicinal materials and functional plants such as the astragalus membranaceus, the angelica sinensis, the ginseng, the lucid ganoderma, the rhodiola rosea and the fructus lycii, the multi-level effects of tonifying qi, enriching blood, activating blood, resisting oxidation and regulating immunity are achieved. The technical effects of comprehensively regulating qi and blood and improving body immunity and resistance are achieved through the synergistic effect of the compound components, compared with a scheme of regulating qi and blood by depending on a single component in the prior art, the compound formula remarkably improves the overall drug effect, solves the problems that a single component is limited in regulating function and insufficient in curative effect, and is suitable for popularization and application. Therefore, the requirements of modern people on comprehensive conditioning and immunity enhancement are met.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.