80 results about "Bursa of Fabricius" patented technology

The invention relates to feed for pheasant juveniles and a preparation method thereof. The preparation method is to grind and mix 30 to 40 parts of corn, 2 to 8 parts of broomcorn, 2 to 10 parts of wheat bran, 15 to 30 parts of bean pulp, 3 to 10 parts of bean cake, 2 to 10 parts of yeasts, 5 to 15 parts of eggs, 6 to 15 parts of fish meal, 1 to 5 parts of bone meal, 0.1 to 1 part of methionine, 0.3 to 1 part of bone meal and 3 to 10 parts of Chinese medicine powder, wherein each 500 grams of Chinese medicine powder comprises 65 grams of astragalus, 100 grams of dandelion, 80 grams of honeysuckle, 80 grams of isatis root, 60 grams of folium isatidis, 30 grams of liquoric root, 30 grams of big head atractylodes rhizome, 30 grams of white paeony root, 20 grams of root of Chinese pulsatilla, 3 grams of borneol and 2 grams of realgar. In the feed for the pheasant juveniles, on the basis of ensuring the sound growth of livestock animals, according to life vulnerability of the pheasant juveniles, the mixing ratio of feed raw material components is reasonable and various Chinese medicine components are added, so that the common communicable diseases such as bursa of Fabricius, white diarrhea and infectious bronchitis of pheasant juveniles are inhibited effectively, and the death rate can reduce to 43 to 47 percent.

The invention relates to a bursal pentapeptide, and the structural sequence of amino acid of the bursal pentapeptide is Cys-Lys-Asp-Val-Tyr. The bursal pentapeptide (Bp5) has simple structure, small molecular weight, no chemical toxicity and no immunogenicity, is easy to be prepared, and can not only be extracted from the bursa of fabricius of chicken or other poultry, but also be prepared by chemosynthesis with quite low cost in large-scale. The bursal pentapeptide (Bp5) can promote the multiplication of T lymphocyte and B lymphocyte, improve the humoral immunity level and cellular immunity level of an organism as well as the capability of anti-peroxidation stress of the organism, is a medicament or preparation with wide application prospect and the functions of immunoregulation, immunotherapy and anti-peroxidation, and can be applied to the fields such as fundamental research, clinical treatment, nutrition and health care, cosmetics, and the like.

The invention discloses a novel chicken infectious bursal disease virusVP 2c DNA (SEQ ID NO: 1), construction of its expression carrier, expressed recombined VP2 protein and the application of said recombined protein in preparing subunit genetic engineering vaccine against chicken infection bursal disease virus. The chicken infectious bursal disease virusVP 2c DNA can be highly expressed in yeast cell, and expressed recombined protein possesses biological activity and immunogenicity of the chicken infectious bursal disease virus natural protein . The expressed recombined protein can be produced into vaccine, and it is demonstrated through immunity chicken test that: the protein subunit vaccine can effectively induce body to generate spcial humoral immune response, make immunity chicken get 90% protection from deadly attack of highly pathogenic vv IBDV, and effectively prevent virus proliferation in the body.

A process for synthesizing the high-purity bursin tripeptide inclues such steps as amino acid protection, synthesizing the protected His-Gly methyl bipeptide, removing amino acid protection of the protected His-Gly bipeptide, synthesizing the protected lys-His-Gly, removing amino acid protection of the protected His-Gly bipeptide, synthesizing the protected lys-His-Gly, removing amino acid protection, synthesizing bursin tripeptide, and purifying.

The invention discloses a pharmaceutical composition with the functions of enhancing the fowl immunity and matching vaccine to enhance the efficacy. The components and the weight percentage thereof are as follows: 0.5 to 5 percent of astragalus polyose, 0.5 to 5 percent of glycyrrhizin, 0.5 to 5 percent of icariine, 0.5 to 3 percent of propolis, 0.02 to 0.1 percent of vitamin E and addition agents. The pharmaceutical composition of the invention can obviously improve both the fowl body cellular immunity and the humoral immunity, and has favorable functions of preventing and controlling the immunosuppressive diseases of the fowl; second, the pharmaceutical composition can be matched with the vaccine to be used to improve the fowl antibody titer, thereby causing the antibodies to be comparatively uniform and to maintain a higher level and showing obvious functions of immunization and efficacy enhancement; and the pharmaceutical composition can be matched with medicines for curing the fowl newcastle disease, the bursa of fabricius, the bird flu and the like, thereby improving treatment effects, increasing the antibody guard time and reducing loss.

The invention discloses a method for quickly building a differential expression gene library. The method comprises the following steps of: firstly extracting total mRNA of infected goose parvovirus and uninfected goose parvovirus goose bursas of fabricius; synthesizing double strands cDNA for enzyme digestion by utilizing the obtained mRNA, and then separating into 2 pipes, respectively adding different adaptors for connecting; carrying out forward subtractive hybridization on the double strands cDNA with the adaptors, then carrying out reverse subtractive hybridization on a forward subtractive hybridization product, carrying out the primary PCR (polymerase chain reaction)) amplification after reverse subtractive hybridization, and carrying out the secondary PCR amplification by taking diluent as a template after diluting an amplification product; and finally carrying out denaturing gradient gel electrophoresis on a product of the second PCR amplification, and dying after electrophoresis to obtain the differential expression gene library of the goose parvovirus stress bursa of fabricius. The gene library built by the method does not have false positive. The method has the characteristics of high speed, high sensitivity, strong specificity, low cost, convenience in operation, and the like.

The invention relates to a preparation method of establishing a reconstructed aviadenovirus for expressing infectious bursal disease virus (IBDV) miRNA, pertaining to the research field of gene engineering and biological product, comprising the selection of siRNA, the synthesis and cloning of double-stranded short-barrette oligonucleotide, the construction of virus transfer vector containing miRNA expression cassette aviadenovirus, the generation and identification of reconstructed virus expressed, the detection of the duplication function by miRNA to IBDV expressed by the reconstructed virus and the inhibition function to genetic expression. Compared with the prior art, the method can not only effectively and persistently express miRNA in bird cells, has specific and persistent inhibition to the duplication of virus and expression of gene of IBDV, but also has not infective function to poultry, which can be applied to in vitro experiments as well as be developed as a novel anti-avain-virus biological product.

The invention aims to provide polypeptide with immunomodulatory effects, namely undecapeptide which has immunomodulatory effects and is separated from bursa of Fabricius. The amino acid sequence of the polypeptide is SEQ ID NO:1. The bursa of Fabricius undecapeptide separated from a bursa of Fabricius extract product has a simple structure, has no toxic or side effect, and has poor immunogenicity. The polypeptide can be obtained by separation and extraction from bursa of Fabricius and also can be obtained by chemical synthesis. The polypeptide has low cost and can be put into mass production. The polypeptide has an induction effect on antibody production, and also can regulate production of cytokines and proliferation of lymphocyte and promote cellular immune response. In addition, the polypeptide also regulates differentiation of B cell in a dose-dependent mode. The polypeptide has a wide application prospect, taking an application of the polypeptide in the aspects of immunomodulation, treatment and the like for example.

The invention discloses a section cDNA in full length of chicken infectious bursal disease virus VP3 cDNA, method for preparing recombined protein by using its expressed carrier, an IBDV indirect ELISA diagnosis method by using said recombined protein and kit prepared with said recombined protein. The invention screens and analyzes the full sequence of chicken infectious bursal disease virus VP3 cDNA, chooses a section cDNA sequence that possesses richful epitope and can highly expressed in prokaryotic cells, establishes prokaryotic expression vector for prokaryotic expression, establishes IBDV indirect ELISA diagnosis method by using expressed recombined protein as coating antigen, and decides the optimal reaction condition. It provides a fast and simple serological identification and diagnosis method for immunity chicken antibody detection and epidemiological investigation. The invention also provides kits taking said recombined VP3 protein as coating antigen, and it is demonstrated that the kit is characterized by good sensitivity, spciality, stability and repeatability.

The invention relates to a veterinary Chinese medicinal composition for improving growth performance and enhancing nonspecific immunologic function of an organism. The composition is prepared from root of Chinese pulsatilla, astragalus, bighead atractylodes rhizome, isatis root, baikal skullcap root, root of Chinese thorowax and rhubarb serving as raw materials. The invention also relates to a veterinary medicament prepared from the veterinary Chinese medicinal composition, a preparation method for the veterinary medicament and application of the veterinary Chinese medicinal composition. The veterinary Chinese medicinal composition of the invention has the effects of strengthening the body resistance to eliminate pathogenic factors, clearing away heat and toxic material and enhancing the nonspecific immunologic function of the organism, and can treat various atypical disease symptoms caused by low immunologic function of the organism, immunologic tolerance, endogenous toxin, stress and the like, the reduction in the growth performance and damp-heat disease symptoms such as wind-heat type cold, flu, bursa of fabricius, intestinal disease syndromes and the like.

The invention relates to a laying hen immune process. According to the specific procedures of the laying hen immune process, Marek vaccine is injected for laying hens which are 1 day old; ND-IB vaccine and triad vaccine are injected for laying hens which are 6 to 7 days old; bursa of fabricius vaccine is injected for laying hens which are 12 days old, bird flu vaccine H5 and henpox vaccine POX are injected for laying hens which are 17 days to 18 days old, bursa of fabricius vaccine is injected for laying hens which are 22 day old, ND-IB vaccine is injected for laying hens which are 26 days old, infectious rhinitis vaccine is injected for laying hens which are 32 days old, ND-IB vaccine and infectious laryngotracheitis vaccine are injected for laying hens which are 39 days to 40 days old, bird flu vaccine H5 is injected for laying hens which are 80 days old, ND-IB vaccine is injected for laying hens which are 90 days old, vaccine H9 and brain pox vaccine are injected for laying hens which are 110 days old, bird flue H5 vaccine is injected for laying hens which are 120 days old, and ND-IB vaccine, triad vaccine and insect expelling vaccine are injected for laying hens which are 130 days old. The laying hen immune process has the advantages that the laying hen diseases can be effectively controlled, and the health of the laying hens is ensured.

The present invention relates to an infectious bursal disease live vaccine production method, which screens virulent strains with good immunogenicity, wider antigen spectrum and immune synergy from studies of characteristics of different infectious bursal disease virus such as immunogenicity, antigen spectrum, pathogenicity of bursa of Fabricius and capacity of breaking through maternal antibody, carries out development of the infectious bursal disease trivalent live vaccine, and develops a live vaccine--an infectious bursal disease trivalent live vaccine which has good immunogenicity, can break through higher level maternal antibody, has long immune duration more than 3 months, and has good protection to the various virulent strains.

The invention discloses a micro-ecological preparation and an application thereof in improvement of production performance, immune performance as well as chicken quality of broiler. The micro-ecological preparation is characterized in that a culture obtained by culturing a complex microbial inoculant through a fermentation medium is used as an active ingredient; the active ingredient of the complex microbial inoculant comprises bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum. The micro-ecological preparation can obviously increase the content of valine in chicken, the content of methionine, the content of lysine, the content of phenylalanine, the content of cystine, the content of tyrosine, the content of proline and the content of crude protein, improve the tenderness and elasticity of pectoral muscles and the chewiness of chicken thigh, decrease the content of cholesterol of chicken, the content of fat, and drip loss, and can obviously increase the crop rate of broiler, meat-to-feed rate, protein, apparent digestibility of calcium and phosphorus, body weight, thymus index, spleen index, bursa of Fabricius index, erythrocyte C3b receptor rosette rate, red blood cell-immune complex receptor rosette rate, the content of protein in serum, the content of alpha-amylase, and the content of cholinesterase.

The invention relates to a method for preparing a yolk immune globulin nanometer liposome for preventing and controlling infectious bursal disease, and belongs to the technical field of medicine preparations of animals. The method for preparing the yolk immune globulin nanometer liposome for preventing and controlling chicken infectious bursa of Fabricius comprises the following steps of: 1, preparing a yolk antibody, namely performing vaccine inoculation on chickens by a multi-time and alternate immune method, and when the agar gel precipitate test (AGPT) detection valence of the yolk antibody is over 1:64, collecting yolk; 2, separating immune globulin, namely diluting yolk suspension by using acetic acid-sodium acetate buffer solution, stirring, standing, centrifuging and reserving supernate; and 3, preparing a liposome, namely preparing a liposome membrane by a membrane dispersion method, adding the yolk immune globulin, rotating and stirring to form a yolk immune globulin liposome capsule, and reforming the formed liposome capsule by using a homogenizer to obtain the nanometer liposome. In the method, the yolk immune globulin is enveloped, and the method has the advantages of simple process, economy, practicability, stable economic performance, good treatment effect and the like.

The invention discloses a traditional Chinese medicine feed supplement and an application thereof. The traditional Chinese medicine feed supplement, which is livestock and poultry feed supplement, comprises the following traditional Chinese medicine raw materials by mass part: 10-60 parts of blueberry, 10-60 parts of Indian buead, 10-60 parts of marine algae, 10-60 parts of honeysuckle and 310-60 parts of oyster. The livestock and poultry feed supplement can reduce the egg cholesterol level, improve the content of selenium, iron and zinc of the egg, as well as the DHA content of the egg and weight of spleen and bursa of fabricius, enhance the relative number of T and B lymphocyte in tonsillas in the thymus, spleen, bursa of fabricius and caecal, and improve the T lymphocyte conversion rate of blood serum of the egg.

The invention relates to a preparation method for a chicken infectious bursal disease specific transfer factor in the technical field of immunomodulators. The method comprises the following steps of: immunizing every healthy domestic fowl by using infectious bursal disease live vaccine B87 strain, B38 strain and infectious bursal disease oil emulsion inactivated vaccine; after antibody level reaches over 24, killing an animal and taking fresh spleen, kidney or bursa of fabricius; crushing tissues, homogenating and repeatedly freeze-thawing for over 8 times; centrifuging to take supernatant; filtering to obtain a component of which the relative molecular mass is less than 5,000 Daltons; and adding a stabilizer EDTA-Na2 (Ethylenediamine Tetraacetic Acid disodium salt) and a composite thermal protectant and disinfecting, wherein the composite thermal protectant is obtained by mixing mannitol, lactose, chitosan, glycerol, glycine and sorbitol according to mass ratio of 2:3:2:3:1:2. The transfer factor has the advantages of high activity, quick response, small dose and no residues compared with a chemical drug product and the like and quick response, convenience in use and transportation, small molecular substance and various application methods compared with a traditional Chinese medicine product and the like.

The invention provides a pharmaceutical composition capable of strengthening the immunity of poultry. The pharmaceutical composition comprises, by weight, from 5 to 15 parts of butafosfan, from 1 to 10 parts of astragalus polysaccharide and the balance adjuvant materials, and the astragalus polysaccharide is extracted by a method including steps of water boiling backflow and ethanol precipitation. By the pharmaceutical composition, both a cellular immunity level and a humoral immunity level of a poultry organism are obviously improved, particularly, the cellular-level immunity of the poultry organism is strengthened, and accordingly the integral immunity of the poultry organism is effectively strengthened. In addition, by using the pharmaceutical composition with a vaccine, avian antibody titer can be increased, the quantity of generated antibodies is high, the antibodies can be maintained for a long time, and an immunopotentiation effect is obvious. Accordingly, the pharmaceutical composition can be used for cooperative medication for avian Newcastle diseases, bursa of Fabricius, avian influenza and the like, antibody protection time is prolonged, the usage and the usage count of vaccines are reduced, and prevention and treatment effects of the vaccines are improved. The pharmaceutical composition can be used for preventing sudden and serious avian diseases, and accordingly prevalence of common avian diseases is avoided.

The invention relates to a fermented traditional Chinese medicament for promoting the growth of livestock and poultry. The fermented traditional Chinese medicament is prepared by the mixing a traditional Chinese medicament, a culture medium and a composite bacterium solution, and performing airtight anaerobic fermentation. The traditional Chinese medicament comprises the following components in parts by weight: 15 to 25 parts of folium artemisiae argyi, 8 to 11 parts of gelsemium elegans, 6 to 12 parts of Chinese wolfberry, 25 to 35 parts of celosia cristata, 30 to 50 parts of paulownia leaves, 40 to 65 parts of pine needle meal, 15 to 25 parts of malt, 5 to 10 parts of folium isatidis, 8 to 16 parts of astragalus, 25 to 40 parts of tangerine peel, 10 to 15 parts of medical stones and 5 to 15 parts of hawthorns. According to the fermented traditional Chinese medicament, the growth of animals is promoted, the balance of intestinal florae is stabilized, kidney enlargement and whitening and liver enlargement caused by various stress such as bursa of fabricius, renal infectious bronchitis, arthrolithiasis and long-term medication are relieved, fatty liver is prevented, ascites syndrome of broilers caused by excessively fast growth is effectively relieved, the immune system is stimulated, and the immunity is improved.

The invention discloses a preparation method of a natural high-effective biological active substance anti-avian infectious bursal disease virus (IBDV) transfer factor. The preparation method comprises the following steps of: inoculating IBDV into an allantoic fluid obtained from a 10-day-aged chick embryo, and preparing IBDV antigen; extracting the IBDV antigen; immunizing a pig with the extracted IBDV antigen; and extracting the anti-IBDV transfer factor from the immunized pig. The inventive anti-IBDV transfer factor can prevent IBDV infection and protect normal body cells from being infected by the IBDV, so as to reduce incidence rate.

The invention discloses a composite fermented traditional Chinese medicine composition for preventing and treating a chicken infectious bursal disease. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 25-40 parts of radix isatidis, 25-40 parts of gynostemma pentaphylla, 30-50 parts of dandelion, 10-30 parts of honeysuckle, 50-70 parts of mugwort leaf, 30-50 parts of herba andrographitis, 1-8 parts of toad-cortex, 2-8 parts of borneol, 5-18 parts of liquorice, and 5-20 parts of munjeet. The traditional Chinese medicine composition is prepared from the following steps: smashing the components in ultrasound; adding fermented culture medium and compound bacteria liquid; conducting enclosed anerobic fermentation at 36-37 DEG C for 12-14 h; adding a stabilizer in the fermented product; conducting vacuum low-temperature drying. The traditional Chinese medicine composition prepared from microbe fermentation acts with the microbe, is converted into components easy to be ingested and utilized by bodies, accelerates treating speed, improves curative effect, and micro-ecology environment of bodies, improves animal food return rate and performance trait, can effectively relieve, eliminate or remarkably relieve symptoms of bleeding, death and the like caused by chicken infectious bursal disease, reduces pathological damage of bursa of Fabricius, milt and the like, relieves potential safety hazards caused by chemical drugs, and improves safety of pharmacy.

The invention discloses a traditional Chinese medicine composition for treating a chicken infectious bursal disease, which is characterized by comprising the following raw materials in parts by weight: 15-20 parts of astragalus membranaceus, 10-15 parts of honeysuckle, 14-18 parts of isatis root, 10-15 parts of liquorice, 8-12 parts of echinacea, 5-10 parts of dandelion, 12-16 parts of poria cocos, 10-15 parts of rhizoma alismatis, 8-12 parts of bambusa sinospinosa, 5-10 parts of lithospermum, 10-15 parts of root bark of the peony tree, 13-17 parts of amur silvergrass, 10-15 parts of alternanthera sessilis and 5-10 parts of broad been skin. The traditional Chinese medicine composition, disclosed by the invention, can remarkably promote repairing of damage to bursa of fabricius of a chicken and redevelopment of the bursa of fabricius; after taking the traditional Chinese medicine composition, the organ coefficient is remarkably increased, the tissue differentiation is obviously repaired, the immunocompetent cells are obviously increased, and the immunological competence is recovered; the traditional Chinese medicine composition has the advantages of stable quality, precise and fast efficacy, high cure rate, capabilities of being completely free from a toxic and side effect and drug residue and the like.

The invention relates to a traditional Chinese medicine composition for treating chicken infectious bursa of fabricius. The traditional Chinese medicine composition comprises the following components by weight: 25 to 35 parts of indigowoad root, 20 to 30 parts of honeysuckle, 20 to 30 parts of phellodendron amurense, 15 to 25 parts of radix rehmanniae, 15 to 25 parts of Chinese angelica, 10 to 20 parts of astragalus membranaceus, 10 to 15 parts of baical skullcap root and 10 to 15 parts of radix glycyrrhizae. The traditional Chinese medicine composition can be used for preventing and treating the chicken infectious bursa of fabricius, has a certain preventing and treating function on other infectious diseases, is reasonable in composition, simple to prepare and convenient to use, and has no medicine residue phenomenon.