The invention provides a preparation method of modified
stropharia rugosoannulata crude
polysaccharide and application of the modified
stropharia rugosoannulata crude
polysaccharide in
lactobacillus fermentation of rhizoma polygonati, and belongs to the technical field of bioengineering. The method comprises the following steps: carrying out
irradiation treatment on crude
stropharia rugoso-annulata
polysaccharide, and then carrying out ultrahigh pressure treatment to obtain the modified crude stropharia rugoso-annulata polysaccharide. In order to solve the problem that the proliferation of
lactic acid bacteria is inhibited in the
fermentation process of rhizoma polygonati, the growth of the
lactic acid bacteria is promoted by adding the modified stropharia rugoso-annulata crude polysaccharide. The method can effectively reduce the inhibition effect of the
polygonatum sibiricum primary pulp on
lactic acid bacteria and improve the
fermentation efficiency. After the
fermentation broth is applied to
polygonatum sibiricum fermentation, the content of irritant components is reduced, the taste and
flavor of primary pulp are improved, the content of saponins, total
phenols and total flavonoids is remarkably increased, and meanwhile the content of
cholesterol is reduced. In addition, the inhibition capability and the
antioxidant activity of the fermented
polygonatum sibiricum primary pulp on alpha-
glucosidase are enhanced, and the comprehensive quality of the product is remarkably improved.