376 results about "Eggs per gram" patented technology

Methods for preparing recombinant proteins, such as antibodies, in eggs are described. The method offers advantages over existing systems for preparing recombinant proteins including high yield, low cost and compatibility with animal protection regulations. In addition, since eggs are edible food sources the recombinant protein does not have to be isolated from the egg.

A method of determining the viability of an egg at least approximately 50% through its incubation period, which method comprises the steps of: (a) causing electromagnetic radiation to impinge upon the egg, the electromagnetic radiation having one or more wavelengths in the infra-red part of the spectrum; (b) receiving at least a part of the infra-red radiation that has passed through the egg and generating an output signal representative of the received infra-red radiation; and (c) processing said output signal to determine whether there is a cyclical variation in the intensity of the infra-red radiation leaving the egg corresponding to action of a heart, the existence of said cyclical variation indicating that the egg is viable; wherein step (a) is performed by directing infra-red radiation so that it passes through the shell for reflection from an outer surface of a vascular structure adjacent an inner surface of said shell, and step (b) is performed by receiving any infra-red radiation so reflected.

The invention relates to methods for determining the sex of birds' eggs. It can be used for an early determination of the sex, in particular in poultry farming, and in this respect also with non-incubated eggs. In the method in accordance with the invention, electromagnetic radiation is emitted onto the blastodisk of an egg by a radiation source and, after a switching off of the radiation source, the decay behavior of the autofluorescence intensity excited by the electromagnetic radiation is detected by a detector at the irradiated region of the blastodisk with time resolution and spectral resolution for at least one wavelength of the autofluorescence. The fractal dimension DF is calculated using the determined measured intensity values and the value of the fractal dimension DF is compared with a species-specific and sex-specific limit value, wherein, when the limit value is exceeded, the respective egg is classified as female and, when it is not reached, the egg is classified as male.

The invention discloses preparation and application of Clostridium butyricum and a live Clostridium butyricum preparation; Clostridium butyricum LXKJ-1 is collected in China Center for Type Culture Collection on 24 March, 2016 under CCTCC NO: M201613. A seed medium, fermentation medium formulation, culture conditions for Clostridium butyricum and a production of a preparation of live Clostridium butyricum are also disclosed. The Clostridium butyricum provided herein is tolerant to acids, bases and high temperature, is highly capable of producing butyric acid, can inhibit animal pathogens, such as Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, and Clostridium perfringens, and can prevent diarrhea in livestock and poultry due to Escherichia coli, Salmonella and Clostridium perfringens, improving intestinal flora balance, promoting the growth of livestock and poultry, relieving constipation in sows, improving egg weight for layers, improving eggshell quality for layers, and decreasing egg-feed ratio.

Determining the viability of an egg by:(a) causing electromagnetic radiation, having one or more wavelengths in the infra-red part of the spectrum, to impinge upon the egg;(b) receiving at least a part of the infra-red radiation that has passed through the egg and generating an output signal representative of the received infra-red radiation; and(c) processing said output signal to determine whether there is a cyclical variation in the intensity of the infra-red radiation leaving the egg corresponding to action of a heart, the existence of said cyclical variation indicating that the egg is viable;wherein step (a) is performed by directing infra-red radiation so that it passes through the shell for reflection from an outer surface of a vascular structure adjacent an inner surface of said shell, and step (b) is performed by receiving any infra-red radiation so reflected.

The invention discloses an egg quality nondestructive detection method based on the unit weight of an egg, and belongs to the technical field of nondestructive detection of the quality of agriculturalproducts. The method comprises the following steps: rapidly connecting and processing the image of every egg, establishing a linear relationship between the area and the volume of the very egg, conveniently and rapidly calculating the unit weight of every egg, and establishing a linear relationship between the unit weight of the egg and the Haugh unit, a common index for the freshness, of the egg to predicate the freshness of the egg. Eggs with the unit weight rho being equal to or more than 1.067 have a high freshness, and are suitable for consumers to eat; eggs with the unit weight rho being 1.046-1.067 can be eaten by the consumers; eggs with the unit weight rho being 0.996-1.046 have a poor freshness, and are not suitable for consumers to eat as shell eggs; and eggs with the unit weight rho being equal to or less than 0.996 cannot be eaten. The freshness of an egg can be rapidly and simply known and the egg is graded only through inputting the image of the egg into a system.

The invention discloses a production method of traditional Chinese medicine healthcare eggs, comprising the following steps of: 1, selecting a reasonable traditional Chinese medicine prescription which is used for preventing and treating the diseases of chicken sand improving the physique and nutritive ingredients of chickens; 2, fermenting the traditional Chinese medicine prescription to obtain fermentation solution and a fermentation body which are used for raising laying hens; 3, selecting laying hens from chicks, and raising by utilizing the fermentation solution and the fermentation bodyprepared in the step 2; and 4, obtaining the eggs laid by the raised laying hens during a laying period, i.e., the traditional Chinese medicine healthcare eggs. The eggs obtained through the production method disclosed by the invention have no hormones or antibiotics, traditional Chinese medicine constituents furthest exert efficacies after being fermented, and the eggs are natural, free from toxic or side effects, and free from medicine resistance; the production method is suitable for large-scale breeding, simple in operation, purely ecological, and pollution-free; and the produced eggs canbe taken as both food and medicines, has healthcare effect and is capable of treating diseases.

The invention relates to a production method of eggs enriched in organic selenium, comprising the following steps: fully stirring and uniformly mixing an feed additive enriched in selenium and a general chicken feed so as to prepare a chicken feed enriched in selenium, which contains 7.5%-45% of the feed additive enriched in selenium and contains 0.3-1.7mg / kg of selenium finally, for standby; feeding chickens with the chicken feed enriched in selenium, and conducting dynamic detection on collected samples every other day until 10 days later when the selenium content in the eggs is 0.3-1.7mg / kg; and continuously feeding the chickens with the chicken feed enriched in selenium, and finally obtaining the eggs with the selenium content being kept at 0.3-1.7mg / kg stably. According to the production method, the selenium content in the egg exists in the form of organic selenium, so that the safety is good, the selenium is easy to absorb and utilize, and the selenium supplementing demands of different crowds can be met.

The invention discloses a preparation method and application of an egg shell membrane composite nano-silver thin film used for an artificial skin. The preparation method comprises the following steps: removing egg white and yolks of fresh eggs and washing egg shells with de-ionized water; separating egg shell membranes and washing the egg shell membranes with the de-ionized water; cutting the egg shell membranes into sizes of (1cm-3cm)*(1cm-3cm) and putting the egg shell membranes into a hydrochloric acid solution with the concentration of 1mM to 100mM and the pH (Potential of Hydrogen) of 1 to 3; immersing for 1 to 4 days at room temperature and washing with the de-ionized water; then putting the egg shell membranes into a dopamine solution with the concentration of 2mg / mL to 4mg / mL and the pH of 8.5 to 9.5; immersing for 6h to 20h at room temperature and washing with the de-ionized water; then putting the egg shell membranes into a silver nitrate solution with the concentration of 10nM to 50nM; immersing for 6h to 20h at room temperature without light, and then washing with the de-ionized water; and naturally drying at room temperature to obtain the egg shell membrane composite nano-silver thin film used for the artificial skin. The prepared egg shell membrane composite nano-silver thin film is used for manufacturing the artificial skin, has excellent mold resistance, and has no obvious biological toxicity; and the preparation method is simple in process, less in consumed time and low in cost.

The invention relates to a method for determining fipronil and fipronil metabolite residual quantity in eggs. The method for determining the fipronil and fipronil metabolite residual quantity in eggs comprises the steps of sample preparation; sample pretreatment; organic solvent extraction; pigment impurity removal through a solid phase extraction column, wherein nitrogen is blown to be almost dry, residues are dissolved through a mobile phase, and the solution is filtered through a filter membrane for UPLC-TQD analysis; standard solution preparation; high performance liquid chromatography-tandom mass spectrometer determination and the like. The method is easy to implement, rapid, high in automatic degree, high in extraction efficiency, good in recovery and repeatability and capable of simultaneously determining the fipronil and fipronil metabolite residual quantity in eggs.

The invention relates to a coating preservation method for eggs. The method comprises the following steps: (1) selecting fresh and crack-free eggs, cleaning the eggs and then adding water to soak the eggs; (2) taking 1 to 2 parts of xanthan gum, 1.6 to 2.05 parts of composite plant essential oil, 0.125 to 0.25 part of licorice flavonoids, 30 to 35 parts of an emulsifier and 50 to 100 parts of ganoderma lucidum polysaccharide, mixing the above-mentioned materials and adding the obtained mixture into water under stirring so as to obtain 1000 parts by weight of an essential oil emulsion; and (3) taking the essential oil emulsion and water according to weight parts, mixing the essential oil emulsion and water so as to obtain a spraying liquid, coating the shells of the eggs with the spraying liquid and drying the eggs at 37 DEG C in the air for preservation. According to the invention, the surface of each egg is coated by a layer of a preservative film which inhibits enzymatic activity of the eggs, alleviates decay of life, achieves the purpose of freshness and quality retention, increases the shelf life of the eggs and saves social cost; moreover, the coated preservative on the surfaces of the eggs is colorless and transparent after drying, so the advantages of safety and low cost are obtained.

The invention discloses a feed additive for producing eggs rich in n-3 series polyunsaturated fatty acid composition. The feed additive adopts an optimized proportion combination by adopting a principle of various plant source function complementation, is added into formula feeds for layer chickens with a ratio of 16 percent, and is used for producing health-care eggs with low cost, safe quality, n-3 series polyunsaturated fatty acid composition enrichment, favorable antioxidant properties, storage stability and easy popularization and application. Compared with ordinary chicken feeds, the feed additive formula enables the n-3PUFA content of a produced egg to be enhanced by 4.01 times to 4.72 times, the level of lipid peroxide (MDA) to be lowered by 20.8 percent to 27.5 percent, and the color of a egg yolk is deepened by 2.1 to 2.4 unit levels (a standard solor fan) when being used for feeding layer chickens. In addition, because added plant resources fulfill has certain efficacy of disease resistance, the feed additive has remarkable effect of nutritional immunity, and the incidence of non-viral diseases of the layer chicken is obviously lowered in a feeding process.

The invention discloses detection on melamine in hen eggs through the inner filtration effect of fluorescence and belongs to the technical field of analytical chemistry. The detection is characterized in that melamine is rapidly detected through the inner filtration effect of fluorescence between a quantum dot (ZnSe QSs) and a silver nano particle (AgNPs). The detection comprises the following steps: stably synthesizing AgNPs through citrate, synthesizing and purifying glutathione modified ZnSe QSs, observing a quantum dot-colloidal silver system of the melamine of different concentrations by using a fluorescence method, and detecting the melamine through fluorescence recovery of the quantum dot. By adopting the method, the melamine in hen eggs can be simply, rapidly and sensitively detected, great sensitivity is achieved, and convenience is brought to later research, production, monitoring and the like.

A method for harvesting biologics from eggs by which an egg is de-capped by positioning the egg in a reference opening so as to expose an upper section of said egg, then, while said egg is positioned within the reference opening, cutting the upper section of the egg by moving a cutter member over the reference opening through the egg, and then removing the debris formed from the cut upper section. The biologics can then be harvested in various ways such as by inverting the egg to allow the biologics to drain for collection. An apparatus for carrying out the method is also provided.

The invention belongs to the field of veterinary drug residue detection, in particular relating to a method for simultaneously detecting residual amoxicillin (AMO), amoxicillin acid (AMA), diketopiperazine amoxicillin (DIKETO) and ampicillin (AMP) in an egg. The method comprises the steps of extracting and purifying egg samples, and detecting the egg samples by taking acetonitrile-0.1% formic acid as a mobile phase by adopting a high performance liquid chromatography-tandem mass spectrometry method. Through the method for simultaneously detecting the residual amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin in the egg as disclosed by the invention, the residue of the amoxicillin, the amoxicillin acid, the diketopiperazine amoxicillin and the ampicillin in the egg can be simultaneously detected, and the invention meets with the European Union standard.

The invention relates to a method for detecting parasitic eggs, which is carried out according to the following steps and is calculated according to the following formula: firstly, livestock feces to be detected are quantified, and water in the volume of 3 to 5 times of the volume of the quantified feces is added so that the feces and the water are stirred into paste; the paste is filtered by a copper screen while washed and filtered by a right amount of water; a filtrate is precipitated for 15 minutes to 60 minutes or is centrifugated, supernatant is discarded, water is added for precipitation, and the procedures are repeated for three times; a right amount of supernatant is discarded, and a measuring cylinder is used for quantifying precipitated liquid; after the precipitated liquid is shaken to be full uniform, a dropper is used for rapidly moving the precipitated liquid to two counter chambers of a counter; and finally, the parasitic eggs in square grids of the two counter chambers of the counter are observed and countered so as to calculate an EPG (Eggs per Gram) value according to the following formula: EPG = (the number of the eggs in the two small square grids of the counter * the volume of the precipitated liquid of the feces) / (the weight of the feces * 0.2). The invention has simple and rapid operation and reliable results, is suitable for detecting most of the parasitic eggs through the feces excretion eggs of people and livestock, and particularly, can qualitatively and quantificationally detect eggs hardly detected so far, such as the eggs of fasciola spp, acanthocephala and distomes, thereby having a wider range of application.

The invention discloses a triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus and a preparation method and application thereof. The preparation method of the egg yolk antibody comprises the following steps of: (1) selecting a hen; (2) performing triple inactivation immunization of the hen obtained by the step (1) by use of newcastle disease virus, infectious bronchitis virus and avian influenza virus; (3) taking the egg laid by the hen and separating the egg yolk liquid; and (4) separating and purifying an egg yolk antibody from the egg yolk liquid obtained by the step (3). The triple egg yolk antibody disclosed by the invention is of great value in treating and preventing newcastle disease, avian influenza and infectious bronchitis.

The invention provides a method for breeding tridentiger trigonocephalus by artificial induced spawning and insemination, which relates to the artificial propagation of gobies. The method required tobe provided for artificially breeding the tridentiger trigonocephalus comprises the steps of ripe fish screening, maturity acceleration, insemination, incubation and larval fish culture, wherein the maturity acceleration of ripe fishes uses luteotropin to release hormone analogues, domperidone maleate and chorionic gonadotropin, and is characterized in that the three hormones are used twice for the maturity acceleration; in the maturity acceleration, the water temperature of culture is between 18 and 21 DEG C, the salinity is between 2 and 6, the dissolved oxygen is between 3.5 and 6.5mg / L, and the pH is between 7.5 and 9.0; and after the second time of the maturity acceleration, in 8 to 10 hours, mature ova and mature sperms are selected for artificial insemination which comprises the following steps that: the activated sperms are poured on the ova for sufficient contact so as to obtain fertilized ova; the fertilized ova are placed in a hatchery pond for incubation; and the hatched larval fish is placed in a culture pond for incubation, and chlorella and yolk powder of eggs are placed in water from the third day after the larval fish is hatched to feed food for the larval fish. The method is used for the artificial breeding of the tridentiger trigonocephalus.

Methods and apparatus for producing vaccine within a plurality of eggs are provided. Each of a plurality of eggs is illuminated with light from one or more LEDs. A detector is positioned adjacent each egg and detects light passing therethrough. Each egg is then identified as containing a live embryo or as being a non-live egg. Each egg that is determined not to contain a live embryo may be removed, either automatically or by hand. A seed virus is injected into each egg identified as containing a live embryo. After a predetermined period of incubation each live embryo is euthanized and amniotic fluid containing a vaccine produced as a result of the presence of a seed virus is harvested from each euthanized egg.

The invention discloses a method and a system for monitoring egg laying performance of a laying hen zone of cage-rearing laying hens. Wherein coops are arranged side by side, a conveying belt is arranged below the coops, and a machine vision detection device with a CCD camera and a lighting device is arranged at the conveying outlet end of the conveyor belt; chicken lay eggs and the eggs leak fromthe bottom of the hencoop and fall onto the conveying belt, after all eggs are laid, the conveying belt is started, the eggs pass through the machine vision detection device in the conveying processalong with the conveying belt, the CCD camera is triggered to shoot and obtain conveying belt surface images with the eggs, and egg contours in the images are obtained through image processing. And counting is performed to obtain the number of eggs, and processing is performed to obtain the coop area position, the egg color, the egg size and the egg weight of each egg, thereby realizing real-timemonitoring. According to the system, the change conditions of the egg laying number, the egg weight and the egg color of each egg laying area every day can be monitored, the egg laying performance andthe health condition of the laying hen area are monitored, the system is simple, and the cost of breeding equipment is saved.

There is provided a computer vision based method for extracting features relating to the developmental stages of Trichuris spp. eggs, wherein for the final developmental stages a larva is present inside the egg, said Trichuris spp. eggs having a substantially oblong or elliptical shape with a protruding polar plug at each end, the shape of the Trichuris spp. eggs thereby defining a longitudinal direction and a transverse direction of the eggs.

The invention discloses a method for preparing an egg yolk antibody, the egg yolk antibody prepared by the method and the application of the egg yolk antibody. According to the technical scheme, the method comprises the following steps of: multiplying viruses by using an apis cerana smith saccular larva as a virus multiplication model; preparing an apis cerana smith saccular larva virus oil adjuvant inactivated vaccine by using the multiplied viruses; continuously immunizing a laying hen for three times by using the prepared inactivated vaccine; measuring the titer of the antibody by an indirect enzyme-linked immune sorbent assay (ELESA); collecting eggs when the titer reaches more than 28; and purifying the egg yolk antibody by a hydration method. The egg yolk antibody is used for treating wasp swarms infected by apis cerana smith saccular larva viruses, has the recovery rate of more than 96.25 percent, is convenient to use, has high specificity and quick response, and does not have relapse and medicinal residues.

The present invention provides in ovo injection devices, including a novel vaccine / substance conservation valve (VCV), a needle depth adjuster (NDA), and methods for selectively delivering vaccines and other substances to eggs in the context of poultry hatcheries. The valve of the present invention allows for the conditional dispensing of vaccines and other substances dependent upon the presence or absence of an egg. When an egg is present, the valve becomes activated, allowing vaccines or other substances to be injected. When an egg is absent, the valve is not activated, thus vaccines and other valuable substances are conserved. The NDA allows embryonated eggs to be more safely injected by reducing the depth of needle penetration, particularly in smaller eggs.

The invention discloses a process of detecting mutation allelomorph of egg fishy odor and an agent case, which can be applied in screening the property of a chicken which lays egg with fishy odor. Chickens whether carry mutation allelomorph of egg fishy odor can be accurately and high effectively differentiated through using the process or the agent case of the invention, thereby solving the problem of egg fishy odor in practical production, greatly reducing the cost of molecule breeding, being beneficial for widespread choosing feed raw material, reducing feed costs and increasing economic benefits. The detecting process or the agent case which are provided by the invention has simple operation, low cost, short detecting time and high accuracy, which can realize automatic detection, and will plays great role in chicken breeding.

The invention relates to a method of monitoring breaking of eggs by an egg breaking apparatus in a cyclic process, where eggs are broken using a plurality of egg breaking devices, each egg breaking device breaking one egg having shell and contents at a time and where the contents of each egg is collected in an egg receiving device, each egg receiving device receiving the contents of only one egg in each process cycle. An operator monitors the contents received in the egg receiving devices and identifies egg receiving devices containing abnormal contents using a pointing device. This identification is detected by a detection device and communicated to a reject mechanism, which causes the contents of the identified egg receiving device to be discharged into a rejected product receptacle. The detection device may be a remote sensor, an integrated part of the pointing device or a device located on each of the egg receiving devices.

The invention provides a method and a device for recognizing native eggs based on machine vision technology. At first, an egg to be tested is photographed, and the egg shape index, the gray value information entropy and the eggshell surface impurity level of the egg to be tested are calculated according to the photograph, , and then egg shape index, gray value information entropy, and eggshell surface impurity level of the egg to be tested are respectively compared with a reference index range, a reference information entropy, and a reference miscellaneous quality step by step, so as to identify the egg to be tested and judge whether the egg is a native egg or not. The egg shape index, the information entropy of gray value and the impurity level of eggshell surface are obtained by machinevision technology, and then compared with the reference value, the native egg can be recognized quickly and the cost of artificial recognition can be greatly reduced. The purpose of recognition can berealized more effectively and the recognition is more accurate by comparing the characteristics of the egg to be tested.

The invention belongs to the field of biotechnology and discloses an egg yolk antibody mouth wash capable of preventing and treating ozostomia and a preparation method of the egg yolk antibody mouth wash. The mouth wash comprises the following components in parts by weight: 1-10 parts of specific complex egg yok antibody preparation, 1-10 parts of a Chinese herb extract, and 100 parts of sterile water. The specific complex egg yolk antibody preparation is a complex antigen prepared from porphyromonas gingivalis, fusobacterium nucleatum and a bacterium internationally named Solobacterium moorei, a laying hen is primarily immunized and then immunized in a booster manner for five times all together, an egg is collected from the first booster immunization, an egg yolk liquid is obtained, separated and purified to obtain a specific complex egg yolk antibody. The egg yolk antibody mouth wash contains both the specific complex egg yolk antibody and an important extract, the breeding of microorganisms in an oral cavity is effectively inhibited, the ozostomia is prevented and treated, the normal flora of the oral cavity is not affected, and the mouth wash is safe and harmless.

The invention relates to a method for the incubation of eggs and to a device for the incubation of eggs. The incubation device includes a cabinet provided with a climate control for regulating the atmospheric humidity and temperature of the air that is to be passed through the cabinet, and at least one frame. At least one stack of trays positioned vertically above one another is arranged in the frame, each tray having a multiplicity of egg positions for receiving an egg. To turn the eggs, the trays of the stack can pivot to and fro through a defined pivot angle about a horizontal axis with respect to the frame. The vertical distance between the trays positioned above one another thereby changes. In the method, the trays are periodically pivoted through the defined pivot angle and the magnitude of the defined pivot angle is variable.

The invention relates to a device and method for observing and recording a chicken incubation experiment. The device comprises a processor, a camera, an egg irradiating device, an incubation support plate, a warmer, a humidifier, a fan, a steering engine, a temperature sensor, a humidity sensor and an oxygen concentration sensor, wherein the processor has a timing function and a timing processing function, the camera is connected with the processor and used for acquiring the images of eggs, the egg irradiating device is connected with the processor and used for irradiating the eggs, the incubation support plate is used for bearing the eggs, the warmer is connected with the processor and used for adjusting environment temperature, the humidifier is connected with the processor and used for adjusting environment humidity, the fan is connected with the processor and used for adjusting environment oxygen concentration, the steering engine is connected with the processor and used for driving the incubation support plate to rotate, the temperature sensor is connected with the processor, the humidity sensor is connected with the processor, and the oxygen concentration sensor is connected with the processor. By the device and method for observing and recording the chicken incubation experiment, an experimenter can clearly observe the development process of egg embryos and monitor and record the development conditions of the embryos in real time, and cost can be saved.

The invention provides a poultry egg freshness comprehensive index detection method based on the visible / near-infrared spectroscopy, which comprises the steps of: R1, sample preparation; R2, spectralinformation acquisition; R3, poultry egg freshness comprehensive index calculation; R4, establishment of a discrimination model; and R5, determination of an optimal prediction model. The invention further provides a poultry egg freshness comprehensive index detection method based on the visible / near-infrared spectroscopy, which is used for implementing the method. Experiments prove that accordingto the poultry egg freshness comprehensive index detection method and device based on the visible / near-infrared spectroscopy, which are provided by the invention, a related coefficient predicted by the determined optimal model can reach 0.868.