537 results about "Blood sugar" patented technology

The invention relates to the technical field of biomedical sensing and health monitoring, and discloses a noninvasive glucose detection system. The system comprises an interstitial fluid extraction device, a photoelectric sensing module and a main control circuit which are integrated on a flexible substrate. The interstitial fluid extraction device drives subcutaneous interstitial fluid to migrate to the hydrogel interface layer under the action of reverse ion electroosmosis; the photoelectric sensing module receives interstitial fluid through a hydrogel interface layer, a working electrode of the photoelectric sensing module comprises a Cu monatomic imprinting recognition layer, and imprinting holes formed by Cu monatomic sites are specifically coordinated with glucose molecules. The master control circuit controls sampling and determines the glucose concentration based on the photocurrent signal. Noninvasive sampling and monatomic imprinting photoelectric detection are fused, sampling-detection integration is achieved, and the device has the advantages of being noninvasive, painless, high in selectivity, high in interference resistance, comfortable to wear and the like and is suitable for continuous monitoring of blood glucose.

The invention belongs to the technical field of bioengineering, and particularly relates to leuconostoc mesenteroides subsp. Mesenteroides FTCM002 and application thereof in preparation of dendrobium officinale leavening with the effects of reducing blood sugar, nourishing the stomach and resisting inflammation. Dendrobium officinale is subjected to enzymolysis and inoculated with the strain for fermentation, and the obtained fermented dendrobium officinale has the effect of inhibiting alpha-glucosidase; the gastric mucosal injury can be relieved, the expression of VEGF, IL-6, IL-8 and TNF-alpha in the gastric mucosal injury is reduced, and the effect of nourishing the stomach is achieved; the cell phagocytic rate can be increased, the NO concentration and the content of immune factors IL-6 and TNF-alpha can be increased, and the effect of enhancing the immunity is achieved; the expression quantity of IL-6, IL-1beta and TNF-alpha in inflammatory cells can be reduced, and the anti-inflammatory effect is achieved.

The invention discloses a hexapeptide with a hypoglycemic effect as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the hexapeptide is Ile-Trp-Asp-Pro-His-Phe, and the amino acid sequence of the hexapeptide is as shown in the specification. The novel hexapeptide IWDPHF with prominent DPP-IV inhibition ability is successfully identified from mulberry leaf proteolysis products through liquid chromatography-mass spectrometry and a virtual screening method, the hexapeptide IWDPHF shows the blood glucose reducing effect superior to that of part of known peptides in an in-vitro enzyme activity inhibition experiment and a zebra fish hyperglycemia model, and no obvious side effect exists; the compound has a good potential of being developed into a hypoglycemic functional product or a drug lead compound. The invention further provides the mulberry leaf peptide with the peptide fragment IWDPHF, and the mulberry leaf peptide can be applied to preparation of drugs or food for reducing blood sugar. The invention provides a new scheme and theoretical basis for treatment of diabetes, and has good market prospect and application potential.

The present application relates to the technical field of oat bran deep processing, and particularly relates to an oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, and a preparation method and application thereof.The present application is prepared by adding alkaline protease and ficin for double-enzyme step-by-step hydrolysis after homogenate, enzymolysis and alkali extraction and acid precipitation pretreatment, and then filtering, ultrafiltration desalination and drying, so that the oat active polypeptide with GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity is obtained.The present application is simple to operate, low in equipment requirement, and suitable for industrial large-scale production.The prepared oat active polypeptide has GLP-1 secretion promotion, blood sugar reduction and lipid reduction activity, has a significant inhibitory effect on pancreatic lipase and alpha-glucosidase, has an obvious GLP-1 secretion promotion effect, and has a significant blood sugar reduction and lipid reduction effect.

The invention discloses a method for preparing various bioactive peptides based on bean dreg fermentation, which comprises polypeptides with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and belongs to the field of microbial biological fermentation. Bacillus amyloliquefaciens sourced from fermented soybeans is used for fermentation, and a mixed product with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity is obtained. The invention also provides a method for purifying the polypeptide with alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, which is characterized in that the mixed product is purified by combined column chromatography, the combined column chromatography comprises cation exchange resin LXP-01, macroporous adsorption resin HP20, weak base anion exchange resin D941, silica gel resin and Sephadex LH-20. The invention also discloses seven bioactive peptides with biological activity, the amino acid sequences are CKLLL, APGYSC, FPKYG, FPGPLV, FPRSH, FMAV and SVCC, and the bioactive peptides have good alpha-glucosidase inhibitory activity, angiotensin converting enzyme inhibitory activity and antioxidant activity, and can be used for producing functional foods, health care products, cosmetics and the like for assisting in reducing blood sugar, reducing blood pressure, resisting oxidation and the like.

A biometric wearable device (e.g. finger ring or smart watch) has optical sensors to measure body oxygenation level, hydration level, glucose level, heart rate, heart rate variability, and / or blood pressure. Light from light emitters is transmitted through body tissue and changes in the light are analyzed. The angles and / or vectors along which the light is transmitted through body tissue can be automatically changed by the device in order to scan different tissue regions and / or different tissue depths.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention relates to the technical field of near-infrared light analysis materials, in particular to a near-infrared spectrum noninvasive blood glucose concentration dynamic detection method which comprises the steps that a plurality of training samples and blood near-infrared spectrum current data of the current blood glucose concentration to be detected are collected, and the training samples are blood near-infrared spectrum historical data of different blood glucose concentrations; determining a water molecule absorption peak value in the blood near infrared spectrum historical data and the blood near infrared spectrum current data, a corresponding wavelength thereof, and a difference between the water molecule absorption peak width, and obtaining the availability degree of each training sample; the mathematical regression model weight of each training sample is obtained based on the availability degree of each training sample and the collection time of each training sample, so that the mathematical regression model weight of each training sample is more reliable and accurate, the accuracy of the mathematical regression model is improved, and finally the accuracy of blood glucose concentration detection is improved.

Provided is an insulin secretion-promoting agent comprising, as an active ingredient, a triglyceride represented by the following formula (I):wherein R1, R2 and R3 each denotes a saturated fatty acid residue, at least one of which is a pentadecanoic acid residue. An insulin secretion-promoting action of the pentadecanoic acid triglyceride at the time of elevated blood glucose level is discovered, and pharmaceuticals and health foods for improving blood glucose level and diabetes are provided using this triglyceride.

Provided is a novel Lactobacillus fermentum strain (Accession No. KCTC 14106BP) and a composition for preventing or treating a metabolic disease including the same. A Lactobacillus fermentum according to the present invention may inhibit fat accumulation and increase anti-inflammatory cells in white adipose tissue. In addition, the Lactobacillus fermentum may effectively inhibit fat accumulation in brown adipose tissue and in liver tissue. The Lactobacillus fermentum strain may exhibit excellent blood glucose improvement effect, and in particular, may reduce a fasting blood glucose level. The Lactobacillus fermentum strain may effectively ameliorate insulin resistance by improving glucose tolerance and increasing insulin sensitivity. Therefore, the Lactobacillus fermentum strain may be usefully used to prevent or treat a metabolic disease such as obesity.

The invention relates to the field of medicine, and discloses a heterocyclic compound with a GLP-1 receptor agonist effect and application of the heterocyclic compound, the heterocyclic compound is a compound with a structure shown in a formula (I), or an isomer, an isotope labeled compound, a prodrug, or pharmaceutically acceptable salt, ester, hydrate or solvate of the compound. The obvious treatment effect is realized in the aspect of reducing blood sugar or reducing weight.

The invention provides a recognition and early warning method and device for irritable hyperglycemia, electronic equipment and a storage medium, and is applied to the technical field of medical data processing. According to the method, data type information (including FreeStyle LibreH dynamic blood glucose and glycosylated hemoglobin data), influence factor data (improved and preset irritable hyperglycemia index and the like) and abnormal state information (fasting interference and detection difference) are taken; acute-stage and chronic-stage average blood glucose basic data are generated through preprocessing. The index weight is enhanced and improved by means of a dynamic fusion engine, the probability is predicted by combining space-time correlation analysis and binary logistic regression, and the abnormal characteristics are obtained by comparing the difference of the AUC by adopting Delog test. The multi-modal features are processed to obtain an identification result, clinical indexes screened by LASSO are fused, index differences are compared to generate risk influence factors, finally, a dynamic early warning result is generated based on a space-time correlation early warning engine and a multi-target strategy, and accurate identification and early warning are achieved.

The invention discloses Talaromyces polyporus ETXG-1-1-1 as well as a culture method and application of the Talaromyces polyporus ETXG-1-1-1, the Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus ETXG-1-1-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 27, 2025, and the preservation number is CGMCC NO: 41927. The radix sophorae tonkinensis endophytic fungus talaromyces polyporus ETXG-1-1-1 is a talaromyces new species, can efficiently secrete extracellular enzymes such as cellulase and protease, can generate stable and high-color-gradation haematochrome, can effectively replace synthetic dyes and secondary metabolites to generate the effects of resisting bacteria, resisting oxidation, reducing blood sugar and the like, and can be used in the industries of textile, papermaking, food processing and the like.

The invention discloses hippophae rhamnoides polysaccharide with the activity of reducing blood sugar and blood fat as well as a preparation method and application thereof. Adding water into the degreased hippophae rhamnoides, adjusting the pH value to be acidic, then heating, stirring and extracting under an ultrasonic condition, and collecting filtrate; and performing post-treatment to obtain the sea-buckthorn polysaccharide. The content of neutral sugar in the sea buckthorn polysaccharide is 25.31%-30.68%, the content of uronic acid in the sea buckthorn polysaccharide is 54.66%-59.79%, and the content of protein in the sea buckthorn polysaccharide is 1.91%-2.14%. According to the method, the sea-buckthorn polysaccharide is extracted by adopting acid assistance-ultrasonic extraction, and the prepared sea-buckthorn polysaccharide has remarkable blood sugar and blood fat reducing activity. When the concentration of the sea-buckthorn polysaccharide is as low as 0.1 mg / mL, the glucose uptake of HepG2-IR cells can still be obviously improved, and the content of total cholesterol and total triglyceride of the HepG2-IR cells can be obviously reduced. The hippophae rhamnoides polysaccharide can be applied to preparation of food or medicine for reducing blood sugar and / or blood fat.

The invention provides a composition for reducing blood pressure, blood fat, blood sugar and uric acid and a preparation method thereof, the composition takes nine kinds of vegetable fat such as acer truncatum seed oil, tomato seed oil and the like as core raw materials, a composite antioxidant, phytosterol and xylooligosaccharide are supplemented, multiple-effect synergy is realized through scientific proportioning, and in the preparation process, the composition is free of toxic and side effects. And by adopting a step-by-step stirring technology, a nitrogen protection technology and a gradient ultrasonic homogenization technology, the biological activity of each component is effectively maintained. A plurality of in-vitro experiments and animal models verify that the composition can significantly regulate blood pressure, blood fat, blood sugar and uric acid levels through the synergistic effect of the raw materials. The raw materials are natural, the active ingredients are completely reserved, and the efficacy is stable, so that the functional health-care product has a wide application prospect in the field of functional health-care product development.

Blood glucose level measurement includes a light source configured to irradiate light to a subject; a monochrome part configured to separate wavelength components of the light that is reflected and scattered from the subject; a light receiver configured to receive the light transmitted via the monochrome part and to generate electrical signals based on the received light; and a processor configured to extract information on the blood glucose level of the subject based on a frequency shift of the light due to the Raman effect.

The invention provides a traditional Chinese medicine composition for reducing blood sugar and blood pressure and a preparation method thereof, and belongs to the technical field of biological medicine, the composition comprises ivory white eggplant extract, parsley extract, banana zucchini rattan extract, coloured bean puffball extract, eucommia ulmoides glycoside and puerarin. The preparation method comprises the following steps: carrying out common enzymolysis on ivory white eggplant by using cellulase, pectinase and papain, and purifying by using an XAD-16 macroporous resin column and a Sephadex LH-20 gel column to obtain an ivory white eggplant extract; the method comprises the following steps: extracting parsley with an ethanol aqueous solution, and purifying with an AB-8 macroporous adsorption resin column to obtain a parsley extract; the preparation method comprises the following steps: extracting banana zucchini rattan by using an ethanol water solution, and purifying by using a D101 macroporous adsorption resin column to obtain a banana zucchini rattan extract; colored bean puffball is subjected to common enzymolysis with chitinase and ficin, extracted with a two-phase solvent and purified with an HPD-100 macroporous adsorption resin column, and the colored bean puffball extract is prepared. All the components are matched to realize synergy of reducing blood sugar and blood pressure.

This invention provides a 3D-printed hollow microneedle array resin patch, its preparation and application, a continuous glucose monitoring device, and a method for dynamic monitoring of blood glucose concentration, belonging to the field of biomedical technology. The hollow microneedle array resin patch of this invention features a "well"-shaped support to ensure the porosity of the perforated microneedles; the design of a hollow conical side opening greatly improves the porosity and microneedle pore volume, effectively increasing the amount of body fluid collected and thus improving detection accuracy. Based on 3D printing technology, the 3D-printed hollow microneedle array resin patch of this invention allows for the determination of basic parameters according to the diameter and height of existing needle sensors, as well as the depth of the stratum corneum and pain nerves, thereby achieving customization of the hollow microneedle combined with the needle sensor structure. It is suitable for needle sensors of different sizes, providing feasibility for microneedles to penetrate subcutaneously for biomarker detection.

This invention discloses a compound peptide nutrient with fat-reducing effects, belonging to the field of health food technology. Specifically, it relates to a VQPIPY peptide or a product containing VQPIPY peptide in the preparation of lipid-lowering drugs or health products, or in the preparation of drugs for treating and / or alleviating alcoholic liver damage. This invention also provides a meal replacement powder containing VQPIPY peptide, shown as a compound of whey powder, milk powder, oats, spinach powder, allulose, and camel milk peptide in a specific ratio. Through the synergistic effect of its components, this product achieves multiple effects such as providing satiety, inhibiting blood sugar, and lowering blood lipids. It is characterized by balanced nutrition, safety, effectiveness, and good taste, and can be used for weight management and daily health maintenance.

The invention discloses a natural product composition and application thereof in reducing uric acid and blood sugar. The composition is prepared from luteolin, verbascoside and curcumin according to the weight ratio of 1: 3: 2. According to the invention, when luteolin, verbascoside and curcumin are combined according to a weight ratio of 1: 3: 2, the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase is the strongest, and the inhibitory activity is obviously superior to the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase when the luteolin, verbascoside and curcumin are independently used, so that the composition has a synergistic effect on reducing the level of uric acid and regulating blood sugar. The discovery provides a theoretical basis and a formula basis for developing novel natural-source functional products for reducing uric acid and reducing blood sugar, and has important application prospects and development values.

The invention belongs to the technical field of medical information processing, and particularly discloses a regression model establishment method and system for detecting related indexes of myocardial cells of Wuzhishan pigs by using blood biochemical indexes, and the method comprises the following steps: collecting blood and heart tissue samples of the Wuzhishan pigs at different month ages, detecting the blood biochemical indexes including liver function, blood fat and blood sugar indexes, and establishing a regression model for detecting the related indexes of myocardial cells of the Wuzhishan pigs. Related indexes of myocardial cells; analyzing the index expression quantity difference, and screening significant difference indexes; then calculating the correlation between the remarkably different blood biochemical indexes and myocardial cell related indexes, and determining high-correlation blood biochemical indexes; and finally, constructing a first version of multiple regression model by respectively taking the myocardial enzyme index and the myocardial cell related index as dependent variables and the high-correlation blood biochemical index as an independent variable, removing outliers and updating the sample set on the basis of the first version of multiple regression model, and constructing a second version of multiple regression model by utilizing the new sample set, and on the basis of the second version of multiple regression model, constructing a third version of multiple regression model by adopting a stepwise regression method. According to the method, related indexes of myocardial cells can be accurately deduced through blood biochemical indexes, and support is provided for Wuzhishan pig heart disease research and model animal research and development.

The invention discloses diglyceride composite edible oil assisting in reducing blood sugar and blood fat and a preparation method thereof, and relates to the technical field of functional edible oil. The diglyceride composite edible oil for assisting in reducing blood sugar and blood fat is prepared from the following raw materials in parts by weight: 40 to 60 parts of high-purity diglyceride oil, 2 to 3 parts of modified flaxseed oil micro powder, 2 to 3 parts of modified camellia oleosa seed oil microcapsule powder, 5 to 8 parts of perilla seed oil, 2 to 5 parts of phytosterol ester, 1 to 3 parts of resistant dextrin, 0.1 to 0.5 part of rosemary extract and 0.05 to 0.15 part of potassium citrate. The compound edible oil is scientific in formula, and the high-purity diglyceride oil is used as a core and matched with various functional components such as the modified flaxseed oil micro powder to synergistically play a role in assisting in reducing blood sugar and blood fat. Through an exclusive modification process, gradient high-pressure homogenization and other standardized preparation processes, the stability and dispersion uniformity of the components are improved, the absorption rate of the active components is high, the storage life is long, no artificially synthesized additive is added, the food is safe to eat, and practicability and health are both considered.

The invention relates to a blood sugar and lipid reducing medicinal and edible compound product as well as a preparation method and application thereof. The compound product is mainly prepared by compounding effective components extracted from the following functional raw materials: eucommia male flowers, eucommia leaves, haws, mulberry leaves, lotus leaves, cassia seeds, astragalus membranaceus, Chinese yams, kudzuvine roots and coix seeds. The preparation method comprises the following steps: (1) pretreatment: combining or respectively carrying out impurity removal, cutting, crushing and sieving on the raw materials; and (2) extracting and concentrating, namely performing multi-stage countercurrent reduced-pressure extraction, cooling an extracting solution, and performing reduced-pressure concentration, or mixing single-raw-material concentrated solutions according to the weight part ratio of the raw materials to obtain a concentrated liquid medicine and food homologous compound product for reducing blood sugar and blood fat. The compound product is directly drunk or used as an additive. The compound product disclosed by the invention has good effects of reducing blood sugar and blood fat and preventing liver cell adiposis and is low in cost. The method can retain effective components to the maximum extent, and is short in extraction time, high in efficiency, easy to separate, simple in process, low in cost and suitable for industrial production.

The application discloses a baicalin ternary co-amorphous drug and a preparation method and application thereof, and the amorphous substance can effectively improve the solubility and dissolution of puerarin and baicalin. The application mixes metformin hydrochloride, puerarin and baicalin according to a molar ratio of 1:1:1, and obtains the baicalin ternary co-amorphous drug through a ball milling method or a solvent-assisted milling method, the preparation method is green and efficient, the co-amorphous substance has high light stability and thermal stability, the solubility is increased by 1.7 times compared with that of a puerarin raw drug crystal, the solubility is increased by 46.8 times compared with that of a baicalin raw drug crystal, and the solubility is reduced by 14.8 times compared with that of a metformin hydrochloride raw drug crystal. The baicalin ternary co-amorphous drug has certain application prospects in liver protection, blood sugar reduction, treatment of damp-heat type colitis and cervical cancer.

The utility model discloses equipment for preparing a peanut membrane extract containing proanthocyanidins with the concentration of more than 50%. The equipment comprises a base, the shell is connected with the base, and a containing cavity is defined by the shell and the base; the filter cartridge is accommodated in the accommodating cavity and comprises a cartridge body which is detachably connected with the base; the top cover is arranged on the cylinder body, the top cover is detachably connected with the cylinder body, an inner cavity is defined, and the inner cavity is used for containing a peanut film; the retaining ring is respectively connected with the barrel and the top cover to limit the top cover to move relative to the barrel; and the driving device is arranged on the base, is in transmission connection with the cylinder body and is used for driving the filter cylinder to rotate along the circumferential direction. The device can prevent expanded peanut membranes from entering filtrate, improves the filtering effect, increases the yield, prepares peanut membrane extracts containing proanthocyanidins with the concentration of more than 50%, and plays roles in regulating blood sugar and body fat, resisting aging and the like.

This invention relates to a photoacoustic diagnostic apparatus and method for measuring blood glucose levels by measuring morphological changes in a membrane caused by sound waves. More specifically, it relates to a photoacoustic diagnostic apparatus and method for adjusting the frequency response of the diagnostic apparatus by adjusting the size of the membrane. According to an embodiment of the invention, the photoacoustic diagnostic apparatus includes: a light source for irradiating a test object with light to emit sound; a plurality of pickup units, each equipped with a membrane that undergoes morphological changes due to the sound; and a measuring unit for measuring the morphological changes of the membrane. The membranes of the plurality of pickup units may have different sizes.

The invention discloses a method for preparing a high-fiber gel minced fillet product based on low-molecular-weight tremella polysaccharide. The method comprises the following steps: modifying the low-molecular-weight tremella polysaccharide; dissolving the modified low-molecular-weight tremella polysaccharide in water to obtain tremella homogenate; and preparing the minced fillet: mixing fish meat with the modified low-molecular-weight tremella polysaccharide, adding table salt and auxiliary materials, and heating and curing to obtain the high-fiber gel minced fillet product. According to the tremella polysaccharide surimi composite product prepared by the preparation method disclosed by the invention, the modified tremella polysaccharide slurry is added in the surimi processing process, so that the gel performance of surimi can be well improved, the surimi has high fiber property, the smooth and elastic mouth feel of the surimi product can be enhanced, and the taste bud experience is richer. Meanwhile, as the tremella polysaccharide has rich biological activity, such as antioxidation, intestinal immunity regulation, blood sugar and blood fat reduction and the like, the tremella polysaccharide can be added into the surimi product as one of the raw materials, and additional functional characteristics can be endowed to the surimi product.

The present application relates to a kind of green tea cell wall polysaccharide with hypoglycemic activity extraction and purification method and the green tea cell wall polysaccharide obtained, and five different tea polysaccharides are extracted by sequential extraction method using water, CDTA, saturated sodium carbonate and 1M NaOH and 4M NaOH solution, and the best hypoglycemic activity of green tea cell wall polysaccharide is screened by sugar metabolism enzyme activity inhibition, and further purified by gel column chromatography.Compared with prior art, the whole operation process of the present application has relatively mild reaction condition, can greatly retain the activity of green tea cell wall polysaccharide and reduce the loss of polysaccharide component to the maximum, has the advantages of high extraction rate, low energy consumption, small solvent consumption, simple operation and low economic cost, etc., lays a foundation for the study of the physicochemical properties and biological activity of green tea cell wall polysaccharide, and has important significance for the utilization of green tea cell wall polysaccharide in food, medical care and cosmetics and other fields.

The application provides a coffee peel enzyme fermentation agent and a preparation method of the enzyme, and belongs to the technical field of enzyme production.The preparation method of the coffee peel enzyme comprises the following steps: mixing coffee peel powder and sugar of sweetener oil residue, and sequentially performing fermentation of Saccharomyces cerevisiae YNXC003 and anaerobic fermentation of Bifidobacterium bifidum YNXC004 to obtain the coffee peel enzyme.The coffee peel powder and the sugar of sweetener oil residue are used as composite raw materials, special bacterial agents are combined, and a segmented fermentation mode is adopted, so that the nutritional activity of the two raw materials can be fully released, the nutritional level and flavor taste of the enzyme can be significantly improved, and the enzyme can be endowed with physiological functions such as blood sugar reduction.The total acid content of the coffee peel enzyme is 8-12 g / L, the total phenol content is 1.5-2.5 g / L, the coffee peel enzyme is rich in bioactive substances such as short-chain fatty acids, polyphenol compounds and amino acids, and has a significant blood sugar reduction effect.

The invention relates to the technical field of biological medicines, in particular to a Chinese wolfberry all-component mixture and application thereof in treatment of diabetes mellitus. The Chinese wolfberry all-component mixture is prepared through the following steps: S1, taking Chinese wolfberry fruits, performing continuous extraction for three times, combining extracting solutions obtained by the three times of extraction, performing filtration, and performing concentration so as to obtain a Chinese wolfberry fruit extract; s2, taking leaf buds, carpopodium and branches of the Chinese wolfberry, performing continuous extraction for three times, combining the three extraction solutions, filtering, and concentrating to obtain a leaf bud / carpopodium / branch extract of the Chinese wolfberry; and S3, mixing the Chinese wolfberry fruit extract obtained in the step S1 with the Chinese wolfberry leaf bud / fruit stem / branch extract obtained in the step S2, filtering, performing graded purification by adopting an ultrafiltration method, intercepting 20-100kDa components, and sterilizing to obtain a Chinese wolfberry all-component mixture. The Chinese wolfberry all-component mixture disclosed by the invention can be used for remarkably reducing a plurality of biochemical index levels such as fasting blood-glucose, glycosylated hemoglobin, triglyceride and extremely low density lipoprotein of a mouse with type 2 diabetes mellitus.