368 results about "Egg laying" patented technology

The invention discloses a fish potential spawning site identification and positioning method based on habitat similarity and spatial autocorrelation, and belongs to the technical field of river ecological protection and habitat evaluation. The method comprises the following steps: step 1, obtaining state values of hydrodynamic force, water quality and substrate indexes of a typical target fish spawning site and target fish distribution information; 2, determining egg laying habitat requirements of target fishes; 3, performing comprehensive habitat suitability evaluation on the typical target fish spawning site; 4, constructing a target fish potential spawning site identification model combining habitat similarity and spatial autocorrelation; and 5, evaluating and verifying the feasibility and accuracy of the method. According to the method, habitat similarity and spatial autocorrelation are combined, a potential spawning site of target fishes in a drainage basin is identified and positioned, and feasibility and accuracy of the method are verified by combining habitat suitability evaluation and spatial position coincidence.

The invention provides application of pulegone in pest prevention and control, and relates to the technical field of pest prevention and control. The pulegone can effectively kill adults of the ampullarian snails under the condition of high concentration, can inhibit spawning of the ampullarian snails and reduce the hatching rate of eggs laid by the ampullarian snails under the condition of low concentration, and can effectively inhibit hatching of the eggs and reduce the hatching rate by spraying egg masses of the ampullarian snails under the condition of low concentration. The plant source component pulegone is adopted to kill the ampullaria gigas, reproduction and breeding of the ampullaria gigas can be effectively destroyed at the same time, prevention and control of the ampullaria gigas are further achieved, and on the basis, pulegone can kill harmful snails such as snails.

The invention discloses a culture method for improving the reproductive capacity of indigenous nematodes and application of the culture method, and belongs to the technical field of indigenous nematode culture. According to the present invention, the Rosselomyces aquimaris L-3 strain is the indigenous nematode intestinal tract strain, is the new strain, can enrich the nutrition source of the indigenous nematode, can significantly improve the egg laying amount of the nematode fed with the strain in the early stage of the breeding period, and can improve the egg laying amount by 36.3% compared with the currently common E. coli OP50 bacteria, and can not produce any harm to the indigenous nematode; the provided culture method is convenient and efficient, the breeding speed of indigenous nematodes can be remarkably increased under the laboratory environment condition by adopting the culture method provided by the invention, and compared with the currently common E. coli OP50 bacteria, the extremely remarkable difference can be achieved in the second breeding day, the necessary nematode number can be provided for short-period experiments, and the related research process can be accelerated.

The invention discloses an SNP molecular marker for identifying the egg laying amount of female sturgeons and application of the SNP molecular marker, and belongs to the field of sturgeon genetic breeding and sturgeon molecular marker-assisted breeding. According to the specific scheme, the SNP molecular marker for identifying the egg laying amount of the female sturgeon is located at the 333bp position of a 17 beta-hsd2 gene nucleotide fragment, the polymorphic form of the SNP molecular marker is C / G, and the genotype of a base mutation site of the SNP molecular marker comprises CC, CG or GG. The female individuals with the genotype of GG belong to low-egg-laying-amount individuals, and the female individuals with the genotypes of CC and CG belong to high-egg-laying-amount individuals. The molecular marker provided by the invention can be used for early artificial breeding of female sturgeon egg laying quantity characters, and can be widely applied to various female sturgeon populations such as acipenser schrenckii, Huso dauricus, acipenser sibiricum and hybrid sturgeons, so that the breeding process of sturgeon varieties with high egg laying quantity is remarkably accelerated.

The invention belongs to the technical field of feed additives, and particularly relates to a 25-hydroxyvitamin D3 premix as well as a preparation method and application thereof, and the 25-hydroxyvitamin D3 premix comprises the following components in percentage by weight: 0.008%-0.08% of 25-hydroxyvitamin D3, 0.08%-0.7% of an emulsifier, 0.3%-1.2% of a stabilizer, 0.8%-1.7% of a synergist and the balance of a diluent. According to the 25-hydroxyvitamin D3 premixing agent, non-water-soluble raw materials are emulsified and coated into water-soluble raw materials, and the 25-hydroxyvitamin D3 premixing agent has the advantages of being simple in process, uniform in water dispersion, better in water solubility and stability and the like. The additive has the effects of improving the bone density of poultry, reducing the occurrence rate of special-shaped eggs in the later egg laying period of laying hens and the like.

The invention discloses a goose egg laying and egg quality detection method based on machine recognition. The method comprises the steps that the initial weight of an egg laying area in different time periods, the goose egg weight and the goose weight corresponding to a goose egg are obtained; based on the goose egg contour line and the goose egg weight, obtaining a goose egg weight parameter; based on the information identifier of the goose entering the egg laying area, obtaining the goose egg weight parameter and a goose weight data set corresponding to the goose egg; and predicting goose egg weight parameters of the gooses in the egg laying area based on the expression state of the gooses entering the egg laying area and the goose weight data set corresponding to the goose eggs to obtain predicted goose egg weight parameters, and determining the egg laying time of the gooses corresponding to the goose eggs based on the goose egg weight parameters, the goose weight data set corresponding to the goose eggs, the egg laying time of the gooses corresponding to the goose eggs and the predicted goose egg weight parameters. And obtaining the goose egg laying quality. The problems that in the prior art, precision is insufficient due to manual goose egg quality detection, and the goose egg laying quality is difficult to detect are solved, and high-precision detection of the goose egg quality and the goose egg laying quality is achieved.

The present invention discloses an automatic determination device and method for the egg-laying performance of individual geese in a small group of family cages. The device comprises an egg-laying detection device, a coding area device, a weighing area device, and a combined goose cage. Through the matched egg detection module, coding module, information storage module, information analysis module and corresponding mark detection algorithm, area division algorithm, target continuous counting algorithm, egg goose matching algorithm, event triggering algorithm, egg-laying detection, egg-laying performance detection, egg-laying collection and other related tasks are performed, which effectively solves the problems of waste of resource space, high cost of separate cage breeding facilities and management, and improves the degree of automation.

The invention discloses a large-scale tea lesser leafhopper cultivation and preservation method suitable for cicada tea production. The method comprises the following steps: step 1, preferably selecting Jinxuan, jade or Fuding white tea as a host plant variety, and determining the specification of the host plant; step 2, establishing a tea lesser leafhopper adult maintenance area, a nymph maintenance area and an egg incubation area, performing partition maintenance, and preferably selecting optimal maintenance conditions and culture density of different maintenance areas; step 3, preferably selecting a collection mode of tea lesser leafhopper eggs: preferably selecting an adult male-female ratio, and preferably selecting egg laying population density, egg collection duration and the like; 4, a tea lesser leafhopper egg storage chamber is established, and storage conditions are optimized; and step 5, preferably selecting incubation conditions and incubation time of the tea lesser leafhopper eggs after low-temperature storage. According to the large-scale production method, the tea branches to be trimmed in the tea garden are utilized, and the cost is low. The tea branches are used for culture, and the death rate caused by the fact that the leafhoppers need to adapt to host plants again during release is reduced.

The utility model discloses a cage rearing laying duck collecting plate structure, a collecting mechanism comprises an upper supporting block, the inner wall of the upper supporting block is provided with a discharging channel, the two sides of the inner wall of the discharging channel are both fixedly connected with cleaning brushes, the cleaning brushes on each side are arranged at equal intervals in the length direction of the discharging channel, and the cleaning brushes on each side are arranged at equal intervals in the length direction of the discharging channel. A positioning shaft is fixedly connected to the lower end of one side of the outer wall of the upper supporting block, a handle is fixedly connected to the side, away from the positioning shaft, of the surface of the upper supporting block, a blocking plate is fixedly connected to the position, located above the handle, of the surface of the upper supporting block, and the side, close to the discharging channel, of the blocking plate is arranged to be an inclined plane; the collecting mechanism is used for collecting eggs laid by the meat ducks in the breeding cage, when laying ducks lay eggs, the duck eggs roll into the discharging channel of the upper supporting block along the guide plate, multiple layers of cleaning brushes are fixed to the inner side of the discharging channel, and therefore when the duck eggs fall, the cleaning brushes scrape away excrement on the surfaces of the duck eggs, and the condition of the duck eggs is improved.

This application discloses a feline allergen Fel d1 egg yolk antibody and its preparation method and application, comprising: obtaining PCR amplification products through PCR amplification; constructing a recombinant plasmid by ligating the PCR amplification products with pET-28a(+); transforming the recombinant plasmid into Rosetta competent cells to obtain transformants; obtaining recombinant antigens by extracting proteins from the transformants; obtaining a subunit vaccine by mixing and emulsifying the recombinant antigens with white oil adjuvant; administering the subunit vaccine to laying hens to obtain immunized egg yolk liquid; adding Tris-HCl buffer and polyethylene glycol to the egg yolk liquid and centrifuging to collect the protein aqueous phase; filtering the protein aqueous phase to obtain a filtrate, adding polyethylene glycol, mixing, centrifuging to collect the precipitate; mixing the precipitate with PBS buffer, adding ammonium sulfate, and centrifuging to obtain the egg yolk antibody. This invention solves the problem that the safety and economy of existing feline allergy treatment methods cannot be simultaneously achieved.

The utility model discloses an egg tray loading machine, and belongs to the technical field of egg machinery. The egg product tray loading machine comprises a rack, and an egg discharging device, an egg receiving tray, a conveying mechanism and a speed reducing mechanism which are arranged on the rack, the egg laying device and the egg receiving support are oppositely arranged; the speed reducing mechanism is arranged on the conveying mechanism; the conveying mechanism comprises a conveying motor, a transmission shaft, a transmission wheel and a conveying belt; the egg receiving support comprises an egg receiving support body, a limiting groove and a baffle. The speed reducing mechanism comprises a speed reducer, an egg placing shaft, an egg placing wheel and a pressing block. According to the tray loading machine, the limiting grooves and the egg placing wheels are matched with each other, the tray loading machine can avoid meaningless movement in the egg product production and conveying process, only when the egg receiving trays receive the egg products, the egg placing wheels can convey the egg receiving trays, the efficiency of the egg products in the production and conveying process is improved, and the production and conveying efficiency of the egg products is improved. And moreover, the energy consumption of the tray loading machine in production can be saved, and the cost is reduced.

The utility model discloses a goose egg laying recording device, which relates to the technical field of poultry farming equipment and comprises an egg laying mechanism, an egg falling mechanism is arranged on the rear side of the egg laying mechanism, a buffer counting mechanism is arranged on the rear side of the egg falling mechanism, a collecting mechanism is arranged at the bottom of the buffer counting mechanism, and the egg laying mechanism comprises an egg laying box. A box inlet inclined plate is fixedly installed below the front side of the egg laying box, a plurality of anti-skid grooves are formed in the top of the box inlet inclined plate, an egg rolling inclined plate inclining backwards is fixedly installed at the bottom of the inner wall of the egg laying box, and an egg outlet groove penetrating through an inner cavity of the egg laying box is formed in the lower portion of the rear side of the egg laying box. The collecting box, the falling egg collecting tank, the recycling water tank, the water outlet hole, the water spraying box, the water suction pump, the water suction hose and the water spraying head are matched with one another, so that the number of each heavy goose egg can be recorded and collected without damage, the integrity of the goose eggs during automatic recording and collecting is effectively improved, and loss is avoided.

The invention belongs to the technical field of molecular biology, and particularly relates to application of a vitellogenin receptor gene VgR, dsRNA of the vitellogenin receptor gene VgR and a primer group to prevention and treatment of fall webworm. The invention provides application of a vitellogenin receptor gene VgR in prevention and treatment of fall webworms. The nucleotide sequence of the vitellogenin receptor gene VgR is shown as SEQ ID NO.1. The invention further provides a preparation method of the vitellogenin receptor gene VgR. The dsRNA is designed by taking the vitellogenin receptor gene VgR as a target gene, so that the hyphantria cunea vitellogenin receptor gene (VgR) can be efficiently silenced, the ovary development is blocked, the egg laying amount is reduced, and finally the effect of preventing and treating hyphantria cunea is achieved. The result of the embodiment shows that compared with a control group, the expression quantity of the VgR gene is obviously reduced after the dsRNA is injected, so that the ovary development is blocked, the egg laying amount is reduced, the number of American white moth generations is reduced to a certain extent, and finally the effect of preventing and treating the American white moth is achieved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular site, a detection primer thereof and application of the SNP molecular site to prediction of egg laying traits of chickens. SNP (Single Nucleotide Polymorphism) typing data comparative analysis of chicken varieties with different egg laying performances is utilized, correlation analysis is performed by combining phenotypic data related to egg laying traits, finally, SNP marker loci remarkably related to the egg laying number of the chicken at the age of 300 days are identified and obtained, the SNP marker loci correspond to chromosomes 61, 577 and 523bp of chicken reference genome GRC7b version sequence information 1 published in an Enmbl website, a basic group is A or G, and an SNP number is rs740376220; the invention further provides a detection primer for detecting the genotype of the SNP molecular site. By adopting the SNP marker site and the detection primer thereof provided by the invention, the egg laying traits of the chickens can be accurately predicted, a technical support can be provided for molecular marker-assisted selective breeding of the egg laying traits of the chickens, the breeding period is shortened, and the breeding efficiency is improved.

The invention discloses a UVB control method and system for laying hen breeding, and the method comprises the steps: arranging a multi-band UVB intelligent light supplement lamp and an illumination intensity sensor in a henhouse, building an intelligent illumination regulation and control module based on a laying hen growth model, and building communication. The system obtains laying hen growth parameters and real-time illumination data, and determines and controls parameters of a light supplement lamp. According to the method, the UVB illumination time, intensity and wavelength can be accurately regulated and controlled according to factors such as different growth stages of laying hens, seasonal changes and breeding environments, and the UVB illumination time, intensity and wavelength can be accurately regulated and controlled according to different growth stages of laying hens, seasonal changes, breeding environments and the like; the method is an innovative method which can be cooperatively controlled with other breeding environment factors, so that the egg laying performance, egg quality and overall health level of laying hens are improved.

The invention relates to the technical field of genetic engineering, in particular to a bacillus subtilis engineering strain as well as a preparation method and application thereof. According to the invention, the bacillus subtilis WB800n is used as an original strain, and the IL-2 gene and the DnaK gene are introduced, so that heterologous expression of the IL-2 is realized, and the yield of the IL-2 is increased. Meanwhile, the IL-2-containing bacillus subtilis disclosed by the invention can be used for improving the immunity and egg laying performance of poultry, and has a good application effect.

The application discloses application of glycyrrhetinic acid as a feed additive to improve the egg laying rate of laying hens. In the laying hen diet of the application, only glycyrrhetinic acid is added, and no chemical synthetic drugs and hormone substances are added, so that the safety of chicken and egg products is guaranteed from the source. The application fully considers the feed cost in application and feed preparation problems, selects glycyrrhetinic acid with low cost, fully plays the medicinal value of traditional Chinese medicinal materials, and the glycyrrhetinic acid is a natural plant extract, has no toxic side effects on laying hens, meanwhile, the preparation and purification method of the glycyrrhetinic acid is simple, the market price is low, and the glycyrrhetinic acid has the advantages of large-scale application.

The invention discloses an antibacterial peptide and application thereof. The antibacterial peptide can inhibit bacteria and can be applied to a feed composition. When the feed is used for feeding the Songyang chickens, the growth performance and the egg laying performance of the Songyang chickens can be remarkably improved, and the feed has a potential application prospect.

The application discloses an evaluation method of egg laying performance of Leizhou black duck. The application is aimed at Leizhou black duck, and finds that 5 SNP sites are significantly related to the age of starting laying of Leizhou black duck; 2 SNP sites are extremely significantly related to the body weight of starting laying of Leizhou black duck. It is further found that a haplotype has low starting laying age, high starting laying body weight and high egg laying quantity, so that individuals with low starting laying age, low starting laying body weight and high egg laying quantity can be screened from the advantageous genotypes of the 7 SNP sites, so as to improve the egg laying performance and economic benefits.

The utility model relates to an egg laying box bottom plate turning plate mechanism which comprises a support and a bottom plate turning plate, the bottom plate turning plate is connected to the support in a rotating mode, a limiting piece is arranged at the end, not connected with the bottom plate turning plate in a rotating mode, of the support, and when the limiting piece limits the bottom plate turning plate, the end, close to the limiting piece, of the bottom plate turning plate is lower than the other end of the bottom plate turning plate. When the egg laying and collecting device is used, the bottom plate turning plate is put down when a lamp is turned on in the daytime, the bottom plate turning plate keeps a certain inclination under the action of the limiting piece, and normal egg laying and collecting are guaranteed; at night, the turning plate of the bottom plate is lifted up to prohibit the chickens from laying eggs, so that the egg laying time of the chickens can be trained, and the egg laying quality and the egg collecting efficiency are improved.

The utility model relates to the technical field of egg laying buffer bottom plates, and discloses a coop body egg laying behavior induction type buffer bottom plate structure which comprises a coop and a buffer bottom plate, a protection mechanism is arranged on the right side of the buffer bottom plate, and the protection mechanism comprises a sliding block, a connecting spring, a telescopic rod and an elastic cushion. The left side of the sliding block and the right side of a buffering bottom plate are fixedly installed. According to the coop body egg laying behavior induction type buffer bottom plate structure, through the use of the buffer bottom plate, the sliding blocks, the buffer grooves, the connecting springs and the telescopic rods, when eggs fall, when the eggs move to the upper surface of the buffer bottom plate, the eggs are inducted, the connecting springs and the telescopic rods are driven to be compressed, automatic induction buffering can be achieved, and the effect of safely collecting the eggs is achieved; and through the use of buffer convex points, a buffer bottom plate and an elastic pad, the problem of how to carry out automatic induction protection during egg collection is solved, and the effect of being safer during egg collection can be achieved.

The utility model relates to a tortrix pupa eclosion and imago feeding and oviposition device which is a transparent box body on the whole, and vent holes are formed in the side wall of the transparent box body; the interior of the box body is divided into a tortrix pupa living area and an adult living area. Wherein the pupa living area is formed by laying fine sand at the bottom of the box body, and tortrix pupae are placed in the fine sand, fly out of the sand surface after development and eclosion in the fine sand and enter the adult living area 3 to mate and lay eggs. An oviposition assembly is arranged in the adult living area and comprises nutrient water, a hygrothermograph and an oviposition collecting strip. Wherein the egg collecting strips are long paper and enter the box body through gaps in the top cover of the box body, and the bottom ends of the egg collecting strips are fixed in the fine sand. Spawns obtained after mating of the female adults can be collected through the spawn collecting strips. According to the utility model, the problem that the small leaf-rolling pests are difficult to observe in hidden life is solved, pupation, eclosion and oviposition are placed in the same space, so that the observation and management are convenient, and the breeding cost is reduced. The device provides test insects and an ecological environment for research on prevention and control of the insects, and also provides a device for viewing the life history of the insects.

The invention discloses a seed production method of a high-yield laying duck auto-sexing commercial line. The accuracy of the method is close to 100% and is obviously higher than that of artificial anal turning for male and female identification; the speed is high, and the production efficiency can be improved by 5-10 times; the labor intensity is low, and fatigue of hands, eyes and the like is avoided; the technical requirement is low; and disease transmission is avoided. According to the seed production method provided by the invention, the low heritability character heterosis is fully utilized, the egg laying performance is high, and the egg yield of 66 weeks old is close to 300; eggshells are green shells and accord with consumption preferences of Chinese people; the feather color of the adult female duck is light brown yellow, slaughtering and processing are facilitated, and the value of the obsolete duck is improved; the mode is suitable for large-scale popularization and application.

The invention discloses laying hen feed immune peptide and a preparation method thereof, and belongs to the technical field of laying hen feed additives. The immune peptide is prepared from the following raw materials in percentage by mass: 29% of threonine, 25% of chili meal, 20% of Chinese prickly ash seeds and 26% of Chinese prickly ash leaf powder. The preparation method comprises the following steps: respectively crushing the raw materials until the particle size is 0.3-0.8 mm, mixing the zanthoxylum bungeanum seed powder, the pepper meal powder and the zanthoxylum bungeanum leaf powder in sequence, and then adding the threonine powder, and mixing for 25-30 minutes to obtain a finished product. The feed has the core advantages that by utilizing the synergistic effect of natural active ingredients in the threonine, the pepper meal, the Chinese prickly ash seeds and the Chinese prickly ash leaf powder, the immune function of laying hens is remarkably enhanced, the morbidity is reduced, meanwhile, the laying rate of the laying hens, the eggshell quality and the egg nutritional value are improved, and the raw materials are natural, free of residues and high in safety.

The utility model discloses an egg laying box for breeding in the technical field of egg laying boxes, which comprises a closed egg laying box body, a top cover of the egg laying box body is provided with a skylight and a side window, and the outer side of each egg laying chamber is provided with an isolation door capable of being turned over inwards to be opened. The step plates can be separately clamped in the clamping grooves between any adjacent clamping tables, a temperature sensor is arranged in each egg laying chamber, the temperature can be detected in time, an independent egg laying chamber can be provided for each hen, the multiple egg laying chambers are integrated in one egg laying box body, a large number of egg laying chambers are effectively arranged in a small space, and the egg laying efficiency is improved. The ventilation openings are formed in the outer side and the top of the egg laying box body, air circulation of the egg laying chamber in the egg laying box body is facilitated, the interior of the egg laying chamber is effectively cooled, stress of hens caused by high temperature is avoided, the isolation door is additionally arranged, the hens cannot open the isolation door again after the isolation door is closed, laid eggs can be isolated, and tracing of family information of the eggs is facilitated.

The duck egg collecting mechanism comprises a breeding cage body and a duck egg collecting frame arranged on the right side of the breeding cage body, an opening and closing door is arranged on the front side of the breeding cage body, and a bottom plate with an inclined angle is arranged at the bottom of the breeding cage body. A connecting plate is arranged at the left end of the duck egg collecting frame, the right end of the connecting plate is of an arc-shaped structure, an arc-shaped rail groove is formed in a gap between the right end of the connecting plate and the right wall of the duck egg collecting frame, and buffering cloth is arranged on the bottom side of the top of the inlet end of the arc-shaped rail groove. A push-and-pull drawer is arranged at the bottom of the duck egg collecting frame, a first supporting plate is arranged at the top of the middle end of the push-and-pull drawer, the bottom of the left end of the first supporting plate is fixedly connected with the top of the second buffering cloth, a collecting groove is formed in the push-and-pull drawer, and the inlet end of the collecting groove is connected with the outlet end of the arc-shaped rail groove. The egg laying of the laying ducks is not influenced.

The invention relates to the technical field of feeds, in particular to a feed capable of increasing DHA of eggs and reducing cholesterol content. Green tea dust and schizochytrium limacinum powder are added into feed to feed laying hens so as to improve DHA of eggs and reduce cholesterol content, and the feed is composed of complete feed and feed additives. The feed additive is composed of green tea dust and schizochytrium limacinum powder. According to the feed capable of increasing DHA of eggs and reducing cholesterol content provided by the invention, the green tea dust and schizochytrium limacinum powder are added into the complete feed to form a novel mixed feed, and laying hens are fed with the novel mixed feed, so that the DHA of eggs laid by the laying hens can be increased, and the cholesterol content of the eggs can be reduced.

The present invention provides a method for selecting breeders that reduces the increment of egg weight before and after laying, and relates to the technical field of laying hen selection. The breeder selection method mainly includes strain breeding, egg number and egg weight statistics, and uses three indicators, namely, the number of eggs laid at 56 weeks of age, the egg weight at 40 weeks of age, and the increment of egg weight before and after laying, to establish a comprehensive selection index, set up a calculation formula, and accurately screen strains and individuals to obtain new varieties or strains of laying hens with high egg production, appropriate egg weight, and small difference in egg weight before and after laying. The present invention overcomes the shortcomings of the prior art, establishes a comprehensive selection index for strains of laying hens, and conducts systematic and scientific seed selection, thereby reducing the increment of egg weight before and after laying, appropriately increasing egg production, maintaining average egg weight, and improving the economic benefits of laying hen breeding.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.