57 results about "Molecular genetics technique" patented technology

The present invention relates to the technical field of molecular genetics, and specifically discloses an application of the pig MDFI gene in regulation of pig skeletal muscle growth and development. According to the present invention, MDFI is overexpressed and interfered in pig skeletal muscle satellite cells, results of cell count and EdU fluorescence detection show that the MDFI can significantly promote proliferation of the pig skeletal muscle satellite cells, and the results of detection on the expression levels of MyoD, myogenin and MyHC expression show that the MDFI can inhibit differentiation of the pig skeletal muscle satellite cells; and with qRT-PCR and Western Blot methods, the regulation effect on the MRFs and the associated cyclin is detected after MDFI is overexpressed and interfered, and the regulation mechanism of the MDFI on the proliferation and differentiation effect of the pig skeletal muscle satellite cells is primarily explored so as to provide the theoretical basis for further research of the MDFI on the pig skeletal muscle growth and development at the body level and provide the reference for improvement of the pork production breeding work.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP molecular marker influencing the wool length character of alpine merino and application. The SNP molecular marker is located at the 24178878th nucleotide site T/C mutation on the 22nd chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the high-mountain merino wool length character, shortening the cultivation period and accelerating the cultivation process, an early selection technology of the high-mountain merino wool length character is established, the breeding time of the high-mountain merino wool length character is shortened, the breeding cost is reduced, and the application value is very high.

The invention relates to the technical field of landscape rice breeding and molecular genetics and particularly discloses a cultivation method and an application of bonsai ornamental rice. The cultivation method comprises the steps as follows: a short stalk cluster mutant and a receptor parent are hybridized to obtain F1; the F1 generation is continuously backcrossed with the receptor parent to obtain a BC1F1 generation, and single plant DNA is extracted at the seedling stage of the BC1F1 generation for molecular mark detection; the BC1F1 generation is continuously backcrossed with the receptor parent to obtain a BC2F1 generation, and single plant DNA is extracted at the seedling stage of the BC2F1 generation for molecular mark detection; the BC2F1 generation and the receptor parent are subjected to advanced backcross to obtain a BCnF1 generation, and single plant DNA is extracted at the seedling stage of the BCnF1 generation for molecular mark detection; a bonsai ornamental rice variety is bred from descendant strains of the BCnF1 after advanced backcross. As detection auxiliary selection is performed by mutant gene molecular markers at the seedling stage of lower generations, thebreeding process is accelerated, and the breeding efficiency is improved; the cultivated short stalk cluster bonsai ornamental rice variety can enrich bonsai types, popularize rice knowledge and deepen understanding of Chinese farming culture.

The invention relates to the technical field of rice resistance breeding and molecular genetics, and particularly discloses a functional molecular marker of a rice nilaparvata lugens resistance gene Bph9, an identification method and application. The molecular marker is Bph9-M, and the nucleotide sequence thereof is shown in a sequence table SIPOSequenceListing 1.0; a Bph9-M primer is a forward primer TCATTAGGAACAGGCTATGCA and a reverse primer TTCTTGTTGACACCGCTCAC. The application of the molecular marker Bph9-M in the breeding of rice nilaparvata lugens resistance includes the following stepsof extracting rice genomic DNA, conducting PCR amplification detection for genotype identification and conducting rice nilaparvata lugens resistance phenotype identification. The molecular marker caneffectively distinguish the Bph9 from other alleles of Bph1, Bph2, Bph7, Bph10, Bph18, Bph21 and the like, be a PCR type intronic functional molecular marker designed for the Bph9 gene, and can carryout rice breeding practice in a simple, rapid and high-throughput manner.

The invention relates to a rice Magnaporthe grisea DNA fingerprint belt-type encoding system classifying and analyzing method, which comprises ill strain acquisition, separating single-spore bacterial strain, extracting genome DNA, expanding rep-PCR, forming fingerprint pattern through electrophoresis, and obtaining encoding pattern based on the fingerprint pattern. The invention realizes fast monitoring for rice bacterium variation by means of molecular genetics, and provides feasible encoding formula for easy reading and comparison of research result at different places.

The invention provides a primer group and a kit for simultaneously amplifying 44 Y-STR gene loci of human and application thereof, and belongs to the technical field of molecular genetics. According to the present invention, the 44 Y-STR gene loci comprise 41 Y chromosome STR gene loci and 3 Y-indel gene loci, including all the gene loci of the mainstream kit in the market; according to the invention, 44 gene loci can be simultaneously amplified in one reaction, the amplification time can be shortened, and the identification efficiency and the detection material adaptability of the kit are improved.

The invention belongs to the technical field of molecular genetics, and particularly relates to an lncRNA marker related to porcine skeletal muscle satellite cell proliferation and application of the lncRNA marker. The lncRNA marker is lnc-88672, and a nucleotide sequence of the lncRNA marker is SEQ ID NO.1. It is found for the first time that the expression of the lnc-88672 is related to the porcine skeletal muscle satellite cell proliferation and is continuously reduced along with the continuous increase of the day age of a pig, so that the lncRNA marker related to the porcine skeletal muscle satellite cell proliferation is provided; the porcine skeletal muscle satellite cell proliferation condition can be identified by detecting the expression of the lnc-88672; the inhibition of the expression of the lnc-88672 can promote the porcine skeletal muscle satellite cell proliferation; and a new idea and method are provided for clinical research of porcine skeletal muscle growth and development.

The invention provides a primer group and a kit for simultaneously amplifying 13 human STR gene loci and application of the primer group and the kit, and belongs to the technical field of molecular genetics. In the invention, the 13 STR gene loci comprise 12 autosomal STR gene loci and one sex identification STR gene locus. According to the method, 13 STR gene loci with fragments smaller than 320 bp can be amplified in one reaction at the same time, 8 core gene loci and 5 preferable gene loci specified by the Ministry of Public Security are included, meanwhile, a sex identification gene locus is further included, and the problem that detection of trace and degraded large-fragment gene loci of a detected material is lost can be effectively prevented. The 13 STR gene loci are combined with mainstream kits in the market, and more gene locus information can be obtained for trace and degraded DNA, so that the individual recognition capability is effectively improved.

The invention discloses gentamycin JI-20B gene engineering bacterium, and construction and application thereof, and belongs to the technical field of biological medicine. Gentamycin JI-20B is directionally synthesized by constructing specific shuttle vector pFD605, utilizing molecular genetics technology to introduce micromonospora, accurately knocking out genB3 gene and genP gene in Micromonospora purpurea by means of frame deletion principle, and permanently inactivating the biological catalysis function of Micromonospora purpurea, utilizing a resistance marker to screen double-crossover mutant strain MicromonosporapurpureaB3P1220 (delta genB3P(gntJI)), performing fermentation on the mutant strain, extracting metabolite, performing structure analysis, and determining that the structure is anastomotic with that of prediction. The constructed engineering bacterium B3P1200 contains no any resistankt markers and the output of gentamycin JI-20B is stable.

The invention provides a primer group and a kit for simultaneously amplifying 34 human STR gene loci and application of the primer group and the kit, and belongs to the technical field of molecular genetics. In the invention, the 34 STR gene loci comprise 29 autosomal STR gene loci, one Y chromosome STR gene locus and 4 sex identification STR gene loci. According to the invention, 34 STR gene loci can be simultaneously amplified in one reaction, 20 core gene loci and 10 preferable gene loci specified by Ministry of Public Security are included, all loci of mainstream kits in the current market are included, and meanwhile, 5 gender identification gene loci are also included; and the risk of gender identification errors caused by Y chromosome deletion can be effectively prevented. 34 STR gene loci are combined, so that the method has the characteristics of high individual recognition capability and high non-father exclusion rate.

The invention discloses a detection kit and a detection method for chicken miRNA-1606 gene polymorphism, and belongs to the technical field of molecular genetics. The detection method comprises the steps of by using chicken whole genome DNA as a template, designing a primer to amplify a target fragment including a miRNA-1606 precursor, purifying and sequencing to know that the 35th site of the chicken miRNA-1606 gene has C or A nucleotide polymorphism; then designing a primer to amplify gene segments containing polymorphism sites and extending reactions by adopting matrix assistant laser desorption ionization-time of flight mass spectrometry in combination with a single primer, and carrying out single SNP site detection on a lot of samples. The detection method has the advantages of high accuracy, sensitive throughput, short detection time and high ratio of performance to cost, can be used for assistant selection and molecular breeding of the chicken, and has important effects for increasing growth traits of the chicken.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker related to influence on the wool length of alpine merino and application of the SNP marker, and the SNP molecular marker is located at the 6296384 nucleotide site T/C mutation on the No.3 chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the wool length of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a technology for selecting the wool length of the high-mountain merino sheep in the early stage is established, the breeding time of excellent characters of the wool length of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention provides a primer group and a kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof, and belongs to the technical field of molecular genetics. In the invention, the 37 Y-STR gene loci comprise 36 Y chromosome STR gene loci and 1 Y-indel gene locus; 37 gene loci can be amplified in one reaction at the same time, the compatibility of current public security DNA database comparison is fully met, the amplification time is shortened, the identification efficiency and the detection material adaptability of the kit are improved, and the multifunctional STR identity identification requirements of current forensic identity identification, forensic DNA database construction and judicial genetic relationship identification can be met.

The invention discloses a method for identifying chicken abdominal fat mass by using a chicken CEBP zeta gene and application of the method, belongs to the technical field of animal molecular genetics, and aims to more accurately and conveniently identify low-fat broiler chickens and accelerate the breeding process of the broiler chickens. Primers are designed according to upstream 123bp and downstream 302bp sequences of the chicken CEBP zeta gene SNP (single nucleotide polymorphism) locus g.764T>G, the obtained primers are used for performing PCR (polymerase chain reaction) amplification on chicken genome DNA, an amplification product is subjected to enzyme digestion by restriction endonuclease, an enzyme digestion product is subjected to agarose gel electrophoresis separation, and a marker genotype of chicken abdominal fat content is obtained. The method is simple to operate, low in cost and high in accuracy, can be used for automatic detection, is an effective molecular marker breeding means, and provides a scientific basis for marker-assisted selection of chickens, and with adoption of the method, acceleration of the broiler chicken breeding process and cultivation of low-fat broiler chickens meeting market requirements are facilitated, so that relatively good economic benefits are obtained.

The invention discloses a haplotype molecular markers related to high fecundity of sheep, a screening method and application, and belongs to the technical field of molecular genetics. The haplotype molecular markers are composed of an SNP1, an SNP2 and an SNP3; the SNP1 is located at the 735bp position of the MTNR1A gene, and the basic group at the position is G or A; the SNP2 is located at the 753bp position of the MTNR1A gene, and the basic group at the position is G or A; and the SNP3 is located at the 845bp position of the MTNR1A gene, and the basic group at the position is C or A. When the tested sheep SNP1 base is G, SNP2 base is G, SNP3 base is C, and they are all homozygous, or when a haplotype is GGC, the sheep to be detected is a high-fecundity individual. The invention provides an effective and accurate molecular breeding marking method for sheep breeding, which can improve the accuracy and breeding process of group selection, and can play a role in molecular breeding of multifetal mutton sheep and multifetal fine-wool sheep.

The invention provides a myristoylated polypeptide for coding mitochondrial localization as well as a preparation method and application thereof, and belongs to the technical field of molecular genetics. The polypeptide sequence has an amino acid sequence represented by an M-N formula, M is an amino acid sequence represented by SEQ ID No. 1, and N is an amino acid sequence represented by SEQ ID No. 2. The invention also discloses application of the polypeptide, a polypeptide composition, a nucleic acid and a polypeptide conjugate in expression of the myristoylated mitochondrial localization protein. The amino terminal of the sequence provided by the invention has a myristoylated conserved sequence which can be myristoylated and then inserted into a mitochondrial cell membrane.

The invention provides a method for identifying the transcription regulation relationship of plant endogenous siRNA, and relates to the technical field of molecular genetics. According to the identification method disclosed by the invention, target genes of plant endogenous siRNA are mined in batches by utilizing a miRNA sequencing and degradation group sequencing data system, so that important information is provided for functional research of the small interfering RNA. According to the identification method disclosed by the invention, a second-generation high-throughput sequencing technologyis fully utilized, high-throughput screening can be carried out on the siRNA and the target genes thereof, and the complexity of a genetic transformation means is overcome; and the method can identify the target genes for siRNA transcriptional regulation more accurately, and has important theoretical significance and practical value for functional research of the siRNA.

The invention discloses application of integrated high-frequency gene loci of high-and-medium-risk HPV related to cervical cancer occurrence, and belongs to the technical field of molecular genetics. Corresponding PCR amplification upstream and downstream primers are designed aiming at integration sites CCAT1, CSMD3, PABPC1P2, RAD51B, TENM2, CSMD1, GRIA3, DPP10, SLC25A51P1, CDH6 and CTNND2 of high and medium risk HPV, and are assembled to obtain the corresponding kit for detecting cervical cancer high risk groups, and the kit has the advantages of rapidness, convenience, accuracy, low false positive rate and the like, is good in innovation and is helpful for clinical diagnosis.

The invention belongs to the technical field of molecular genetics, and particularly relates to a method for amplifying a rice aroma gene Badh2 sequence. The method for amplifying the rice aroma gene Badh2 sequence comprises the steps that 1, a specific primer set with the sequence shown in the SEQ ID No.1-26 is included; 2, basic group substitution or deficiency or addition is performed; 3, a polymerase chain reaction is performed in a PCR amplification system to obtain the rice aroma gene Badh2 sequence. The method has the advantages of being simple, rapid, low in technical requirement and accurate in result identification, can be used for identifying rice aromatic varieties and rice non-aromatic varieties, can also be used for identifying directive breeding rice aromatic varieties, can be used for genetic identification of conventional rice and bridge materials and plays an important role in various processes of molecular marker directive breeding rice varieties.

The present invention relates to mutant DNA polymerases, their genes and their uses. More specifically, the present invention relates to mutant DNA polymerases which are originally isolated from Thermococcus sp NA1. strain and produced by site-specific mutagenesis, their amino acid sequences, genes encoding said mutant DNA polymerases, their nucleic acids and PCR methods by using thereof. As mutant DNA polymerases according to the present invention have decreased proofreading activity and changed function of inosine sensing effectively compared to wild type DNA polymerase, PCR using primers with specific nucleic acids has made rapid progress. Therefore, the present invention is broadly applicable for PCR in various molecular genetic technologies.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker for identifying the age-round shearing amount of alpine merino and application of the SNP molecular marker. The SNP molecular marker is located at the 20077683th nucleotide site G/C mutation on the fifth chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the annual shearing amount of the alpine merino sheep, shortening the breeding period and accelerating the breeding process, an early selection technology of the annual shearing amount of the alpine merino sheep is established, the breeding time of the excellent character of the annual shearing amount of the alpine merino sheep is shortened, the breeding cost is reduced, and the application value is very high.