100 results about "Molecular genetics technique" patented technology

The invention discloses an STON2 gene intron SNP (Single Nucleotide Polymorphism) molecular marker related to egg laying traits of chickens and application of the STON2 gene intron SNP molecular marker, and belongs to the technical field of molecular genetics. The research finds that the STON2 gene may participate in the related process of poultry sexual precocity and egg laying, so that the reproductive performance and the egg laying character of the chicken are influenced, and four SNP loci remarkably related to the egg laying character of the chicken are found in the fifth intron region and the sixth intron region of the STON2 gene for the first time. Based on the SNP site, the invention further develops an SNP molecular marker related to the egg laying traits of the chickens, and the SNP molecular marker can be used for auxiliary breeding of high-yield laying hens.

The invention discloses an InDel molecular marker related to wheat yield traits and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker is a molecular marker InDel 509; the nucleotide sequence of the molecular marker InDel 509 is as shown in SEQ ID No. 1. A wheat TaPRR95-5A gene functional marker InDel 509 is used for carrying out PCR (Polymerase Chain Reaction) amplification, polyacrylamide gel electrophoresis detection is carried out, and a germplasm material carrying a Hap II type is screened out according to the size of a target band. The functional marker can provide an effective marker resource for wheat genetic improvement and molecular marker-assisted breeding.

The invention discloses a KASP molecular marker related to brassica napus quality traits and application of the KASP molecular marker, and belongs to the technical field of molecular genetics. According to the KASP molecular marker related to the brassica napus quality traits, the primers of the KASP molecular marker and the application of the KASP molecular marker and the primers in brassica napus genetic typing or quality traits identification, results show that the KASP molecular marker can accurately identify genotypes, is high in sensitivity and specificity and effectively reduces the error rate. The invention also discloses a brassica napus genotyping / quality identification method, and the result shows that the material with the genotyping result of AA has the characteristics of low erucic acid content, low thioglycoside content, high oleic acid content, high linoleic acid content, low arachidonic acid content and low protein content; gG-type materials exhibit high erucic acid, high thioglycoside, low oleic acid, low linoleic acid, high arachidonic acid and high protein content. Individuals with excellent quality characters can be rapidly screened in early generations, and the long process of character identification after plant maturation in traditional breeding is avoided.

The invention relates to the technical field of molecular genetics, and particularly discloses a Yr6 gene, protein, a recombinant vector and application of the Yr6 gene, the protein and the recombinant vector, the full-length sequence of the Yr6 gene is shown as SEQ ID NO.1, and the CDS sequence of the Yr6 gene is shown as SEQ ID NO.2. Yr6 is overexpressed in a transgenic mode to improve wheat stripe rust resistance. The Yr6 gene provided by the invention is beneficial to improving the resistance of wheat to stripe rust, and provides support for breeding of stripe-rust-resistant wheat.

The invention discloses a molecular marker combination related to a cockscomb height character and a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the molecular marker combination and the detection reagent thereof, ten SNP loci remarkably associated with the chicken first-laying crown height and the 130-day-old crown height are identified in the intron of the CTCF gene for the first time, based on the SNP loci, the molecular marker combination related to the cockscomb height character and the detection reagent thereof are designed, and the molecular marker combination and the detection reagent thereof can be used for early screening of laying hens with high cockscomb height and can be used for early screening of laying hens with high cockscomb height. The method is of great significance to breeding and breeding of precocious high-quality laying hens.

The invention is suitable for the technical field of molecular genetics, and provides an SNP molecular marker related to pig backfat thickness and application thereof. The invention studies and determines that the pig backfat thickness related molecular marker is located at the 95583333rd site (corresponding to the 701st basic group A / G mutation of SEQ ID NO: 1) of the No.18 chromosome of the international pig reference genome version 11.1, and verifies the influence effect of the molecular marker on the pig backfat thickness character. And when the basic group at the site is A, the pig has lower back fat thickness. On the basis, a molecular marker-assisted selection breeding technology for rapidly improving the backfat thickness character of the pig is established, the frequency can be increased generation by generation by optimizing the dominant allele (A), the backfat thickness of the breeding pig is remarkably reduced, the genetic improvement process of commercial pig herds containing landrace and large white pig blood lineage is accelerated, and the breeding efficiency is improved; the sales profit is increased for enterprises, the core competitiveness is improved, and the economic benefits of commercial pigs are improved.

The invention discloses a molecular specific marker for identifying genetic sex of culter alburnus and application of the molecular specific marker, and relates to the technical field of animal molecular genetics. The specific preparation method comprises the following steps: firstly, extracting culter alburnus genome DNA; then screening by utilizing the genomic DNA of the culter alburnus to obtain a culter alburnus genetic sex molecule specific marker; the method comprises the following steps: designing a primer according to a culter alburnus genetic sex molecule specific marker, and amplifying a culter alburnus genome sample to be detected; if the PCR amplification product is a single bright specific band, the individual is a female individual, and if the PCR amplification product has two bands, the individual is a male individual. The genetic sex of the culter alburnus can be quickly and accurately identified by utilizing the method, so that the method has important scientific value on the research on the sex mechanism of the culter alburnus, and has important significance and application value on the sex control and selective breeding of the culter alburnus.

The application discloses PRR30 gene SNP and a haplotype combination thereof in identifying bull semen quality, and belongs to the technical field of molecular genetics. Eight SNP sites are identified, wherein SNP7: g.794 C>A or SNP8: g.2247 G>T can be used for identifying the high and low of bull semen collection amount, SNP2: g.-2151 C>T can be used for identifying the high and low of bull sperm abnormality rate, and the haplotype combination of SNP1-SNP8 is that the bull with H1H11 has higher semen collection amount and lower sperm abnormality rate, and can be further used for identifying bull semen quality, thereby helping staff to evaluate bull breeding performance in an early stage, to carry out superior gene bull screening, to promote the cultivation of superior bull, to shorten the screening period and to improve the breeding efficiency.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

The invention belongs to the technical field of molecular genetics, and relates to a stichopus japonicus whole life history on-site rapid sex determination molecular marker, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The stichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a kit, an identification method and the like. The molecular marker and the developed kit can be used for rapid sex identification of the stichopus japonicus in the whole life history, and the problem of accurate sex identification in stichopus japonicus culture is solved.

This invention relates to the field of molecular genetics, specifically to a SNP molecular marker closely linked to a major QTL related to bolting in spinach and its application. The SNP molecular marker provided by this invention is KMBL29, with its polymorphic site located at position 47693313 on spinach chromosome 1, exhibiting an A / G polymorphism. A genotype of AA corresponds to bolting resistance in spinach; genotypes of AG or GG correspond to bolting susceptibility. This invention also provides specific primers based on KASP technology for genotyping this SNP molecular marker. This molecular marker and primers can be used to breed high-quality spinach varieties resistant to bolting, shortening the breeding cycle.

The invention discloses a molecular marker related to correction day age of 100 kg weight of a pig and application of the molecular marker, and belongs to the technical field of molecular genetics. According to the invention, for the first time, two SNP loci obviously associated with the weight correction age of a pig reaching 100 kg are identified in a CDS region of a GREB1 gene, and the two SNP loci show complete linkage imbalance and form a conservative haplotype block. The two mutation sites are significantly related to the corrected day age (Plt; 0.001) of the 100 kg weight of the pig, and the corrected day age of the GA genotype is significantly shorter than the corrected day age of the GG genotype and the AA genotype. Detection of the molecular marker associated with the correction day age reaching 100 kg is beneficial to breeding of meat varieties / lines with high growth speed, and has important significance for breeding of pig varieties.

The application discloses a SNP molecular marker combination of a FGF9 gene promoter region related to chicken egg laying traits and application thereof, and belongs to the technical field of molecular genetics. The application finds that multiple SNP sites exist in the FGF9 gene promoter region of a chicken through screening of SNPs between breeds, and for the first time, carries out correlation analysis on the SNPs genotypes of a Jining Hundred-day chicken population and egg laying traits, finds that the GA ‑2177 genotype individual corresponds to more maximum consecutive egg laying numbers; the GA ‑2289 genotype individual corresponds to an earlier age of first egg laying; and the GA ‑3906 genotype individual corresponds to more total egg laying numbers. Therefore, through detection of the three molecular markers related to the egg laying traits, not only is the method simple and fast, but also it is helpful to breed a high egg laying chicken breed, and provides favorable help for breeding work.

The present invention discloses SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs, their primers and applications, belonging to the technical field of molecular genetics. The SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs disclosed by the present invention have amplification primer sequences as shown in SEQ ID NO.1 and 2. The SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs and their amplification primers can be applied to the population structure analysis of Corydalis bulbocapniodes, the analysis of markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs, the selection of excellent germplasms of Corydalis bulbocapniodes and the preparation of a kit for screening excellent germplasms of Corydalis bulbocapniodes with high sub-bulb weight, and can be directly used for screening excellent germplasms, gene mapping and cloning, and molecular marker-assisted breeding. Moreover, they have chromosome mapping information, which is beneficial to further fine mapping and cloning of genes controlling the fresh weight trait of sub-bulbs, and is of great significance in the breeding of high-yield Corydalis bulbocapniodes.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

The invention belongs to the technical field of molecular genetics, and particularly relates to application of a QTL molecular marker in wheat thousand grain weight breeding. The QTL marker is named as QTgw.saas-5B, the QTL marker is positioned on a 5B chromosome, and the physical position of the QTL marker on IWGSC RefSeqv2.0 is 554.52 Mb to 576.35 Mb. The QTL molecular marker disclosed by the invention can be used for finely positioning the thousand seed weight of wheat, so that a new way is provided for wheat breeding.

The application discloses a SNP molecular marker combination related to corn kernel traits and application thereof, and belongs to the technical field of molecular genetics. The application develops a SNP molecular marker related to the hundred-kernel weight of corn by studying the relationship between a Zm00001d014723 gene and the kernel weight of corn, and further realizes early prediction of the yield of inbred lines through direct and rapid identification of seedlings by PCR amplification and agarose gel electrophoresis, so that a new thought is provided for molecular breeding.

The application belongs to the technical field of molecular genetics, and particularly relates to a gene related to corn kernel quality and application, and a nucleotide sequence of the gene is shown as SEQ ID NO. 1. The gene provided in the application has the function of regulating the content of asparagine, tryptophan, phenylalanine, histidine, arginine and serine in corn kernels, and the content of amylose, amylopectin and total starch. The knockout mutant of the gene created by gene editing technology increases the content of asparagine, tryptophan, phenylalanine, histidine, arginine and serine in corn kernels, and the content of amylose, amylopectin and total starch, and unexpectedly reduces plant height and ear height, which is beneficial to simultaneously increasing corn yield and quality, and improving variety density tolerance.

The present invention belongs to the technical field of molecular genetics, and particularly relates to the application of an oat AsEFT1 gene and its encoded protein. The oat AsEFT1 gene is characterized in that the CDS nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence encoded by it is shown in SEQ ID NO.2. The present invention discovers and clones the AsEFT1 gene for the first time. Through Arabidopsis experiments, it is confirmed that it can regulate the flowering trait, providing a new molecular marker for oat breeding and having great market application value.

The invention belongs to the technical field of plant molecular genetics, and particularly discloses an InDel molecular marker related to the short fruit character of bitter gourd fruits and application of the InDel molecular marker. According to the InDel molecular marker, a long-fruit bitter gourd inbred line '23C57' and a short-fruit bitter gourd inbred line '23C58' are selected as parent materials, a genetic population containing four generations (P1, P2, F1 and F2) is constructed, and the molecular marker is obtained. A BSA (Bovine Serum Albumin) positioning and linkage positioning combined method is used for detecting and controlling genetic loci of bitter gourd fruit types, and InDel are respectively identified at a 22136954 locus and a 22076609 locus of a bitter gourd No.6 chromosome through sequencing analysis and are respectively named as L070 and L901. The invention provides a theoretical basis for molecular breeding of bitter gourd fruit types, and enriches molecular marker resources.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker related to identification of sheep tail fat deposition and application of the SNP marker, and the SNP molecular marker is located at the 4146459th nucleotide site C / A mutation on the No.15 chromosome of AR-UIRamv3.0 reference genome (GCF016772045.2). The technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. The SNP locus detection is used for carrying out thin tail type sheep early selection, shortening the breeding period and accelerating the breeding process, an fat tail type sheep early selection technology is established, the breeding time of fat tail type sheep with excellent characters is shortened, the breeding cost is reduced, and the application value is very high.

The invention discloses application of an HAVCR2 gene in cattle molecular marker-assisted selective breeding, and belongs to the technical field of molecular genetics. The method comprises the following steps: by taking cattle genome DNA as a template, amplifying a cattle HAVCR2 gene copy number variation region by utilizing a specific PCR primer, amplifying a cattle BTF3 gene partial segment as an internal reference, and finally calculating and judging the individual copy number variation type by utilizing a 2 * 2-delta delta Ct method. The method provided by the invention lays a foundation for establishing association between cattle HAVCR2 gene copy number variation and growth and development, can be used for cattle molecular marker-assisted selective breeding work, and is convenient to popularize and apply.

The invention belongs to the technical field of molecular genetics, and particularly discloses a rice blast resistance gene Pijx specific functional marker primer which is an upstream primer Pijx-KF and a downstream primer Pijx-KR aiming at a resistance haplotype of a rice blast resistance gene Pijx, the upstream primer Pijx-KF is shown as SEQ ID NO.1, and the downstream primer Pijx-KR is shown as SEQ ID NO.2; the upstream primer Pijx-GF and the downstream primer Pijx-GR aim at the non-resistant haplotype of the rice blast resistance gene Pijx, the upstream primer Pijx-GF is as shown in SEQ ID NO.3, and the downstream primer Pijx-GR is as shown in SEQ ID NO.4. The invention also discloses a kit for detecting the rice blast resistance gene Pijx by using the upstream primer Pijx-GF and the downstream primer Pijx-GR. The marker primer provided by the invention is suitable for identifying and screening resistant haplotype Pijx and non-resistant haplotype Pijx in rice breeding, can distinguish homozygous and heterozygous resistant haplotype Pijx during molecular marker-assisted selection, and can screen a new resistant haplotype Pijx antigen from rice germplasm resources.

The invention discloses a functional molecular marker of a bacterial blight resistance gene Xa7, a screening method and application of the functional molecular marker, and belongs to the technical field of molecular genetics. The inDel molecular marker of the bacterial leaf blight resistance gene Xa7 is Xa7-InDel, and the molecular marker of the bacterial leaf blight resistance gene Xa7 is Xa7-InDel; the Xa7-InDel is a molecular marker which is amplified from genome DNA (Deoxyribonucleic Acid) of a rice variety carrying the bacterial blight resistance gene Xa7 through a primer pair SEQ ID NO.1 and SEQ ID NO.2, and the molecular marker and the bacterial blight resistance gene Xa7 are in a specific banding pattern; the InDel molecular marker disclosed by the invention can be used for detecting bacterial leaf blight resistance genes in rice varieties, and can be simply, conveniently and quickly applied to rice disease-resistant breeding practice with high throughput, so that the disease-resistant breeding process is accelerated.

The invention discloses an InDel molecular marker related to maize rough dwarf disease resistance and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker closely linked with the maize rough dwarf virus resistance gene is obtained by screening in 12, 857, 147-13, 124 and 718 bp regions of the maize chromosome 2, the resistance of maize to rough dwarf virus can be accurately identified in an early stage by utilizing the InDel molecular marker, and the InDel molecular marker has the advantages of simplicity, convenience, rapidness, high efficiency, accuracy, good repeatability, high specificity and the like; the method can be used for corn molecular marker-assisted breeding.

The invention belongs to the technical field of plant molecular genetics, and particularly discloses an InDel molecular marker for identifying bitter gourd sex and application thereof.The specific InDel molecular marker is screened out by constructing sequencing data of a strong female parent mixed pool P1 (23C37), a strong male parent mixed pool P2 (23C38), F1 and F2 BAS mixed pools and combining bioinformatics analysis, and the molecular marker is used for identifying bitter gourd sex. The excavated InDel molecular marker sites are different from annotation genes of homologous pistil genes reported in cucurbitaceae species in the past, when the InDel molecular marker is used for identifying the strong female characters of the bitter gourds in the seedling stage, detection is easy and convenient, the requirements for instruments, equipment and the technology are low, the detection can be conducted on an electrophoresis technology platform, and the method is suitable for large-scale popularization and application. Sex typing can be completed in the seedling stage (3-4 true leaf stage), and an efficient tool is provided for sex identification of bitter gourds and other cucurbitaceae crops.

The invention relates to the technical field of plant molecular genetics, in particular to a TaMCT gene for regulating wheat grains and application of the TaMCT gene. The nucleotide sequence of the wheat TaMCT gene is as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The invention also discloses an application of the wheat TaMCT gene in regulation and control of wheat grains. Experiments prove that the wheat TaMCT gene can regulate and control wheat grains and improve the wheat yield, so that the wheat TaMCT gene plays an important role in regulating and controlling the wheat grains, and new gene resources and breeding resources are provided for wheat breeding.

The application discloses a molecular marker combination related to a comb height character, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. CTCF Ten SNP sites significantly related to comb height at laying and comb height at 130 days old are identified in introns of genes, and based on the SNP sites, the application designs the molecular marker combination related to the comb height character and the detection reagent, which can be used for early screening of egg hens with high comb height, and has important significance for breeding and selection of high-quality egg hens with sexual precocity.

The application discloses a kind of genetic diversity evaluation of Ziziphus silvestris and its application of SSR molecular marker combination, it is related to molecular genetics technical field.The SSR molecular marker combination includes molecular marker NSZ-43385, NSZ-9141, NSZ-37910, NSZ-240, NSZ-22900, NSZ-39803, NSZ-42088, NSZ-165, NSZ-26522, NSZ-39039, NSZ-41051, NSZ-1007, NSZ-36575, NSZ-37045, NSZ-34772 and NSZ-45650.The SSR molecular marker combination can be used for genetic diversity evaluation of Ziziphus silvestris, carries out genetic diversity research of different population of Ziziphus silvestris, and has important guiding significance to aspects such as formulating Ziziphus silvestris germplasm resource effective protection and sustainable utilization strategy.