42 results about "Molecular genetics technique" patented technology

The invention discloses a molecular marker combination related to a cockscomb height character and a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the molecular marker combination and the detection reagent thereof, ten SNP loci remarkably associated with the chicken first-laying crown height and the 130-day-old crown height are identified in the intron of the CTCF gene for the first time, based on the SNP loci, the molecular marker combination related to the cockscomb height character and the detection reagent thereof are designed, and the molecular marker combination and the detection reagent thereof can be used for early screening of laying hens with high cockscomb height and can be used for early screening of laying hens with high cockscomb height. The method is of great significance to breeding and breeding of precocious high-quality laying hens.

The invention discloses a chicken FMNL1 gene promoter region molecular marker, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. According to the invention, two SNP loci significantly associated with egg laying performance are identified in a chicken FMNL1 gene promoter region, and the two SNP loci generally exist in a plurality of different varieties of chickens and present a consistent effect trend between different genotypes. Based on the SNP locus identified by the invention, the invention develops and designs a chicken FMNL1 gene promoter region molecular marker and a corresponding detection reagent. The method can be used for accurately identifying the laying day age and egg laying traits of the chickens in the early stage, and has important significance on breeding of high-yield laying hens.

The invention belongs to the technical field of molecular genetics, and relates to a stichopus japonicus whole life history on-site rapid sex determination molecular marker, a kit and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1 in a sequence table. The stichopus japonicus with the molecular marker is male. The invention also provides a primer for amplifying the molecular marker, a loop-mediated isothermal amplification detection primer group, a kit, an identification method and the like. The molecular marker and the developed kit can be used for rapid sex identification of the stichopus japonicus in the whole life history, and the problem of accurate sex identification in stichopus japonicus culture is solved.

The invention discloses a molecular marker related to correction day age of 100 kg weight of a pig and application of the molecular marker, and belongs to the technical field of molecular genetics. According to the invention, for the first time, two SNP loci obviously associated with the weight correction age of a pig reaching 100 kg are identified in a CDS region of a GREB1 gene, and the two SNP loci show complete linkage imbalance and form a conservative haplotype block. The two mutation sites are significantly related to the corrected day age (Plt; 0.001) of the 100 kg weight of the pig, and the corrected day age of the GA genotype is significantly shorter than the corrected day age of the GG genotype and the AA genotype. Detection of the molecular marker associated with the correction day age reaching 100 kg is beneficial to breeding of meat varieties / lines with high growth speed, and has important significance for breeding of pig varieties.

The application discloses a SNP molecular marker combination of a FGF9 gene promoter region related to chicken egg laying traits and application thereof, and belongs to the technical field of molecular genetics. The application finds that multiple SNP sites exist in the FGF9 gene promoter region of a chicken through screening of SNPs between breeds, and for the first time, carries out correlation analysis on the SNPs genotypes of a Jining Hundred-day chicken population and egg laying traits, finds that the GA ‑2177 genotype individual corresponds to more maximum consecutive egg laying numbers; the GA ‑2289 genotype individual corresponds to an earlier age of first egg laying; and the GA ‑3906 genotype individual corresponds to more total egg laying numbers. Therefore, through detection of the three molecular markers related to the egg laying traits, not only is the method simple and fast, but also it is helpful to breed a high egg laying chicken breed, and provides favorable help for breeding work.

The invention belongs to the technical field of molecular genetics, and particularly relates to a wheat powdery mildew resistance main effect QTL, a KASP molecular marker closely linked with the wheat powdery mildew resistance main effect QTL and application of the wheat powdery mildew resistance main effect QTL. The invention provides a wheat powdery mildew resistance major QTL, the major QTL locus is located at the end part of a wheat 1A chromosome short arm and is located in a 4.4 cM genetic interval between flanking KASP markers Chr1A6275522K2 and Chr1A11524652K9, nine KASP molecules are developed, auxiliary selective breeding and screening of powdery mildew resistance wheat strains can be carried out, and a new available molecular marker is provided for screening of wheat powdery mildew resistance materials.

The invention discloses an InDel molecular marker related to maize rough dwarf disease resistance and application of the InDel molecular marker, and belongs to the technical field of molecular genetics. The InDel molecular marker closely linked with the maize rough dwarf virus resistance gene is obtained by screening in 12, 857, 147-13, 124 and 718 bp regions of the maize chromosome 2, the resistance of maize to rough dwarf virus can be accurately identified in an early stage by utilizing the InDel molecular marker, and the InDel molecular marker has the advantages of simplicity, convenience, rapidness, high efficiency, accuracy, good repeatability, high specificity and the like; the method can be used for corn molecular marker-assisted breeding.

The application discloses a molecular marker combination related to a comb height character, a detection reagent and application thereof, and belongs to the technical field of molecular genetics. CTCF Ten SNP sites significantly related to comb height at laying and comb height at 130 days old are identified in introns of genes, and based on the SNP sites, the application designs the molecular marker combination related to the comb height character and the detection reagent, which can be used for early screening of egg hens with high comb height, and has important significance for breeding and selection of high-quality egg hens with sexual precocity.

The application discloses a kind of genetic diversity evaluation of Ziziphus silvestris and its application of SSR molecular marker combination, it is related to molecular genetics technical field.The SSR molecular marker combination includes molecular marker NSZ-43385, NSZ-9141, NSZ-37910, NSZ-240, NSZ-22900, NSZ-39803, NSZ-42088, NSZ-165, NSZ-26522, NSZ-39039, NSZ-41051, NSZ-1007, NSZ-36575, NSZ-37045, NSZ-34772 and NSZ-45650.The SSR molecular marker combination can be used for genetic diversity evaluation of Ziziphus silvestris, carries out genetic diversity research of different population of Ziziphus silvestris, and has important guiding significance to aspects such as formulating Ziziphus silvestris germplasm resource effective protection and sustainable utilization strategy.

The invention provides an SCAR molecular marker for identifying sex of cannabis sativa, a primer group, a kit and application, and belongs to the technical field of plant molecular genetics. Aiming at the problems of difficulty in early sex identification of cannabis sativa and insufficient stability of the existing molecular marker, the SCAR marker developed based on the Y chromosome specific segment has high specificity and stability, can be used for rapid and accurate identification of sex of cannabis sativa in different growth periods, and has important application value for improving the production efficiency of medicinal cannabis sativa related products.

The application discloses a chicken TRAF7 gene promoter region SNP marker and application thereof in chicken breeding and belongs to the technical field of molecular genetics. The application finds that a SNP marker exists in the chicken TRAF7 gene promoter region through screening of SNPs between breeds, the nucleotide sequence of the SNP marker is shown as SEQ ID No. 1, and the 122th base from the 5' end of the sequence shown as SEQ ID No. 1 is a SNP site, the base of which is A or T. The SNPs genotype of Jining hundred-day chicken population is firstly correlated with the egg laying trait, and it is found that the individual with the TT ‑896 genotype corresponds to an earlier age of first egg laying; the individual with the TT ‑896 genotype corresponds to a higher maximum number of continuous egg laying, therefore, the molecular marker associated with the SNP site and the egg laying trait is detected, which is not only simple, fast and convenient, but also helps to breed a high-egg-yielding chicken breed and provides favorable help for breeding work.

This invention discloses a promoter region SNP marker combination, detection reagent, and its application related to chicken egg production performance, belonging to the field of molecular genetics technology. This invention is the first of its kind in... CHST8 Three SNP sites significantly associated with egg production performance were identified in the promoter screening of the gene. All three are located within key regulatory regions of the promoter and possess the potential to influence transcription factor binding and gene transcription activity. Furthermore, these three SNP sites exhibit a consistent effect trend across different genotypes, meaning that superior genotypes show better performance in key traits such as egg production. In marker-assisted breeding, consistency and stability of effect direction are often more practically significant. Therefore, the promoter region SNP marker combinations of this invention are of great importance for breeding high-producing laying hens.

The present application relates to the technical field of molecular genetics, and particularly relates to a kind of molecular marker affecting oat crude protein content and application thereof, the base sequence of the SNP molecular marker is as shown in SEQ ID No.1, and the 301th site in the sequence of SEQ ID No.1 is T or C.The present application overcomes the defects of time and energy consumption, low accuracy and lack of effective related molecular markers in the prior art oat high crude protein variety breeding relying on traditional phenotype selection, realizes the rapid and accurate prediction and screening of oat crude protein content, improves the breeding efficiency of oat high crude protein variety, and shortens the breeding cycle.

The application provides a MNP marker site for identifying a fast-growing Litopenaeus vannamei population, a primer set and application thereof, and belongs to the technical field of molecular genetics of aquatic animals. The MNP marker site, the corresponding primer set and the kit thereof can screen the fast-growing Litopenaeus vannamei population, and it is proved that the MNP marker site can realize high-throughput analysis, avoid a large number of non-existent false genotypes, reduce detection cost and analysis complexity, and the accuracy of identifying the fast-growing Litopenaeus vannamei population is as high as 100%. The MNP marker site and the application thereof provided by the application have reference significance for the field related to molecular genetics and genotype analysis of aquatic animals.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.

The invention discloses a ZNF318 gene molecular marker related to a pig backfat thickness character and application of the ZNF318 gene molecular marker, and belongs to the technical field of molecular genetics. According to the invention, a mutation site g.38376064 (Ggt; A) significantly associated with the backfat thickness of 100 kg of a pig is screened and identified in a CDS region of the ZNF318 gene. Based on the mutation site g.38376064 (Ggt; a), a ZNF318 gene molecular marker and a detection primer pair related to the pig backfat thickness character are designed, according to the genotype of the ZNF318 gene molecular marker at the SNP site, the 100 kg backfat thickness character of the pig can be accurately identified, a pig individual with the large backfat thickness or a pig individual with the small backfat thickness is selected according to actual needs, and the pig individual with the large backfat thickness or the small backfat thickness is selected according to the actual needs. And beneficial help is provided for pig breeding work.

This invention proposes a SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage, and its application, belonging to the field of animal molecular genetics. It includes an SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage. The SNP marker uses the sequence shown in SEQ ID NO.1 as its starting sequence, with position 51 being either A or G. If the SNP is of the GG genotype, it indicates a broiler with high abdominal fat percentage. Replacing A with G at position 51 promotes preadipocyte differentiation in chickens. It is mainly used to promote preadipocyte differentiation in chickens and identify broilers with high abdominal fat percentage.

This invention discloses a molecular marker in the promoter region of the RSPO3 gene associated with egg production traits in chickens and its application, belonging to the field of molecular genetics. This invention detected three SNP loci significantly associated with egg production traits in the promoter region of the chicken RSPO3 gene: starting weight (LW), total eggs laid at 52 weeks of age (E52), and longest consecutive laying days (LCS). These SNP loci are g.-1,831, g.-2,515, and g.-2,760. Based on these three SNP loci, molecular marker combinations associated with egg production traits can be developed. Detecting these molecular marker combinations can aid in the breeding of high-producing hens.

The application discloses an InDel molecular marker closely linked to maize brittle stalk and application thereof, and belongs to the technical field of molecular genetics. The InDel molecular marker is InDel-NR53; is located on a corn chromosome 3; compared with a wild type, there is an insertion of 1480 nucleotides between 203349918bp-203349919bp of the chromosome 3; and the nucleotide sequence of the molecular marker InDel-NR53 is shown as SEQ ID No. 1. The InDel molecular marker can be used for detecting and identifying maize brittle stalk, and can be simply, quickly and high-throughput used in breeding practice, and can accelerate the breeding process of maize.

The application discloses application of a bovine heat adaptability related SNP molecular marker and belongs to the technical field of molecular genetics. The heat adaptability of a bovine is evaluated by detecting the genotype of the bovine heat adaptability related SNP molecular marker. The SNP molecular marker is g.84324020 C>G, is located on an EDIL3 gene, and the sequence of the EDIL3 gene is defined with reference to the region from 83843439 bp to 84324060 bp in NCBI:NC_037334.1. The heat adaptability of a bovine with the SNP molecular marker genotype of GG is obviously superior to that of a bovine with the SNP molecular marker genotype of CC or CG, and the latter has better adaptability to a low-temperature environment. The SNP molecular marker can be used for efficiently screening bovine individuals with heat adaptability, has important guiding significance for breeding and genetic improvement of heat-resistant bovine breeds, provides a high-quality technical scheme for characteristic germplasm resource cultivation, and significantly shortens a breeding cycle.

This invention relates to the field of molecular genetics, disclosing SNP molecular markers associated with the mottled leaf phenotype of spinach and their applications. The SNP molecular markers contain a nucleotide sequence with a G / A polymorphism at position 158522852 bp on spinach chromosome 3; spinach plants with the G base have normal leaves, while those with the A base exhibit mottled chlorotic leaves. This invention utilizes segregating population analysis (BSA) to discover SNPs associated with the mottled leaf phenotype of spinach and develops a KASP primer combination for detecting the molecular markers for assisted breeding. The three KASP primers provided by this invention can detect the mottled leaf phenotype of spinach at the seedling stage, with high throughput and high accuracy. The co-dominant KASP markers provided by this invention facilitate the screening of spinach varieties containing the mottled leaf gene, significantly accelerating the breeding process of spinach varieties containing the potential mottled leaf gene.

The invention discloses a molecular marker related to wheat septoria leaf blight resistance, a resistance gene and application of the molecular marker and the resistance gene, and belongs to the technical field of molecular genetics. According to the present invention, by using the diversified wheat population covering the wide geographical source (particularly including the Chinese local variety), the major STB resistance QTLs capable of being stably represented in different environments and used for marker-assisted selection can be efficiently and accurately identified, and the key candidate gene can be further clarified, such that the major STB resistance QTLs can be used for marker-assisted selection; therefore, an efficient breeding tool and a clear genetic target are provided for cultivating new wheat varieties with lasting and broad-spectrum resistance and strong adaptability.

The application discloses application of a bovine heat adaptability related SNP molecular marker and belongs to the technical field of molecular genetics. The heat adaptability of a bovine is evaluated by detecting the genotype of the bovine heat adaptability related SNP molecular marker. The SNP molecular marker is g.84324020 C>G, is located on an EDIL3 gene, and the sequence of the EDIL3 gene is defined with reference to the region from 83843439 bp to 84324060 bp in NCBI:NC_037334.1. The heat adaptability of a bovine with the SNP molecular marker genotype of GG is obviously superior to that of a bovine with the SNP molecular marker genotype of CC or CG, and the latter has better adaptability to a low-temperature environment. The SNP molecular marker can be used for efficiently screening bovine individuals with heat adaptability, has important guiding significance for breeding and genetic improvement of heat-resistant bovine breeds, provides a high-quality technical scheme for characteristic germplasm resource cultivation, and significantly shortens a breeding cycle.

This invention discloses a molecular marker, detection reagent, and their applications that are closely linked to the stage-specific regulatory trait of maize leaf color, belonging to the field of molecular genetics. This invention is the first to screen and identify an InDel molecular marker on maize chromosome 6 that is closely linked to the stage-specific regulatory trait of leaf color in maize seedlings. Using this marker, genotypic identification of maize populations can be accurately performed in the early stages of breeding, efficiently screening individuals carrying the leaf color regulatory gene. These materials gradually exhibit an albino phenotype before V5, and then naturally revert to green. The leaf color mutant involved in this invention only exhibits albinoness during the seedling stage and does not continuously express this trait throughout the entire growth period, having a minimal impact on maize growth and yield. Applying this InDel molecular marker to maize hybrid seed production and purity identification can achieve rapid and accurate screening of true and false hybrids before sowing or during the seedling stage, significantly improving the purity of hybrids.

The invention discloses an SNP molecular marker combination related to egg quality, a detection reagent and application of the SNP molecular marker combination and the detection reagent, and belongs to the technical field of molecular genetics. According to the invention, analysis is carried out based on large population data of 3388 Wenshui green-shell laying hens, 30 SNP molecular markers significantly related to egg quality are screened, the molecular markers cover multiple dimensions such as egg weight, eggshell quality and internal quality, and the problems of single molecular marker and poor specificity in existing research are solved; the method provides a universal technical framework and an accurate genetic target for the cultivation of a new strain of high-quality laying hens, has a wide industrial application prospect, and is of great significance in promoting the modernization development of the laying hen breeding industry in China.

The application discloses an antagonistic SNP molecular marker related to chicken abdominal fat and bone traits and application thereof, and belongs to the technical field of animal molecular genetics. In order to more accurately and conveniently select low abdominal fat content broilers in breeding, while giving consideration to meat production and growth performance, the application estimates genetic parameters of abdominal fat rate and six growth traits of a 12-week-old F2 generation resource population of Northeast Agricultural University chickens, then carries out GWAS analysis to screen significant SNPs in the genome, and finally carries out multiple comparisons on least square means of different genotypes of the screened SNPs. The results show that the abdominal fat rate is negatively correlated with the six growth traits, and a total of 14 SNPs exhibit antagonistic pleiotropy, and four SNPs exhibit synergistic pleiotropy on the six growth traits. The SNPs provided by the application can be used for molecular marker assisted breeding of broilers, and individuals can be directly selected at the genome level in early stage, so that the breeding process is accelerated.

The invention relates to the technical field of molecular genetics, and discloses an STS molecular marker of wheat gibberellic disease resistant major QTL and application of the STS molecular marker, the STS molecular marker is located on a long arm of a wheat 2D chromosome, a primer group of the STS molecular marker comprises a forward primer and a reverse primer, and nucleotide sequences of the forward primer and the reverse primer are shown as SEQ ID NO.1-2. The STS molecular marker of the wheat gibberellic disease resistance main effect QTL is closely linked with gibberellic disease resistance, the diagnosis accuracy is high, and the STS molecular marker can be widely applied to auxiliary breeding of gibberellic disease resistance wheat.

The invention discloses a TJAP1 gene molecular marker related to growth traits of large white pigs and application of the TJAP1 gene molecular marker, and belongs to the technical field of molecular genetics. According to the invention, a mutation site g.38501283 (Ggt; C) in a 3'regulation region of a TJAP1 gene is preliminarily screened from a large white pig population, wherein the mutation site g.38501283 (Ggt; C) is obviously associated with the backfat thickness of a pig with the weight of 100 kg and the day age of the pig with the weight of 100 kg. Based on the mutation site g.38501283 (Ggt; C), a TJAP1 gene molecular marker and a detection primer pair related to the growth traits of the large white pigs are designed, pig individuals with excellent growth performance such as low backfat thickness, 100 kg weight, short day age and the like can be efficiently screened out, and technical support is provided for rapid breeding of good varieties of the large white pigs.