12 results about "Molecular genetics technique" patented technology

The application discloses a SNP molecular marker combination related to egg quality, detection reagents and application thereof, and belongs to the technical field of molecular genetics. Based on the analysis of data of 3388 Wenshui green-shell egg chicken large groups, 30 SNP molecular markers significantly related to chicken egg quality are screened, which cover multiple dimensions such as egg weight, egg shell quality, internal quality and the like, and solve the problem of single molecular marker and poor specificity in the prior art; a universal technical framework and precise genetic target point are provided for new strain breeding of high-quality egg chickens, and have a wide industrial application prospect, and have important significance for promoting the modernization development of the egg chicken breeding industry in China.

This invention discloses a promoter region SNP marker combination, detection reagent, and its application related to chicken egg production performance, belonging to the field of molecular genetics technology. This invention is the first of its kind in... CHST8 Three SNP sites significantly associated with egg production performance were identified in the promoter screening of the gene. All three are located within key regulatory regions of the promoter and possess the potential to influence transcription factor binding and gene transcription activity. Furthermore, these three SNP sites exhibit a consistent effect trend across different genotypes, meaning that superior genotypes show better performance in key traits such as egg production. In marker-assisted breeding, consistency and stability of effect direction are often more practically significant. Therefore, the promoter region SNP marker combinations of this invention are of great importance for breeding high-producing laying hens.

The present application relates to the technical field of molecular genetics, and particularly relates to a kind of molecular marker affecting oat crude protein content and application thereof, the base sequence of the SNP molecular marker is as shown in SEQ ID No.1, and the 301th site in the sequence of SEQ ID No.1 is T or C.The present application overcomes the defects of time and energy consumption, low accuracy and lack of effective related molecular markers in the prior art oat high crude protein variety breeding relying on traditional phenotype selection, realizes the rapid and accurate prediction and screening of oat crude protein content, improves the breeding efficiency of oat high crude protein variety, and shortens the breeding cycle.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.

This invention proposes a SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage, and its application, belonging to the field of animal molecular genetics. It includes an SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage. The SNP marker uses the sequence shown in SEQ ID NO.1 as its starting sequence, with position 51 being either A or G. If the SNP is of the GG genotype, it indicates a broiler with high abdominal fat percentage. Replacing A with G at position 51 promotes preadipocyte differentiation in chickens. It is mainly used to promote preadipocyte differentiation in chickens and identify broilers with high abdominal fat percentage.

The application discloses application of a bovine heat adaptability related SNP molecular marker and belongs to the technical field of molecular genetics. The heat adaptability of a bovine is evaluated by detecting the genotype of the bovine heat adaptability related SNP molecular marker. The SNP molecular marker is g.84324020 C>G, is located on an EDIL3 gene, and the sequence of the EDIL3 gene is defined with reference to the region from 83843439 bp to 84324060 bp in NCBI:NC_037334.1. The heat adaptability of a bovine with the SNP molecular marker genotype of GG is obviously superior to that of a bovine with the SNP molecular marker genotype of CC or CG, and the latter has better adaptability to a low-temperature environment. The SNP molecular marker can be used for efficiently screening bovine individuals with heat adaptability, has important guiding significance for breeding and genetic improvement of heat-resistant bovine breeds, provides a high-quality technical scheme for characteristic germplasm resource cultivation, and significantly shortens a breeding cycle.

This invention relates to the field of molecular genetics, disclosing SNP molecular markers associated with the mottled leaf phenotype of spinach and their applications. The SNP molecular markers contain a nucleotide sequence with a G / A polymorphism at position 158522852 bp on spinach chromosome 3; spinach plants with the G base have normal leaves, while those with the A base exhibit mottled chlorotic leaves. This invention utilizes segregating population analysis (BSA) to discover SNPs associated with the mottled leaf phenotype of spinach and develops a KASP primer combination for detecting the molecular markers for assisted breeding. The three KASP primers provided by this invention can detect the mottled leaf phenotype of spinach at the seedling stage, with high throughput and high accuracy. The co-dominant KASP markers provided by this invention facilitate the screening of spinach varieties containing the mottled leaf gene, significantly accelerating the breeding process of spinach varieties containing the potential mottled leaf gene.

The application discloses application of a bovine heat adaptability related SNP molecular marker and belongs to the technical field of molecular genetics. The heat adaptability of a bovine is evaluated by detecting the genotype of the bovine heat adaptability related SNP molecular marker. The SNP molecular marker is g.84324020 C>G, is located on an EDIL3 gene, and the sequence of the EDIL3 gene is defined with reference to the region from 83843439 bp to 84324060 bp in NCBI:NC_037334.1. The heat adaptability of a bovine with the SNP molecular marker genotype of GG is obviously superior to that of a bovine with the SNP molecular marker genotype of CC or CG, and the latter has better adaptability to a low-temperature environment. The SNP molecular marker can be used for efficiently screening bovine individuals with heat adaptability, has important guiding significance for breeding and genetic improvement of heat-resistant bovine breeds, provides a high-quality technical scheme for characteristic germplasm resource cultivation, and significantly shortens a breeding cycle.

This invention discloses a molecular marker, detection reagent, and their applications that are closely linked to the stage-specific regulatory trait of maize leaf color, belonging to the field of molecular genetics. This invention is the first to screen and identify an InDel molecular marker on maize chromosome 6 that is closely linked to the stage-specific regulatory trait of leaf color in maize seedlings. Using this marker, genotypic identification of maize populations can be accurately performed in the early stages of breeding, efficiently screening individuals carrying the leaf color regulatory gene. These materials gradually exhibit an albino phenotype before V5, and then naturally revert to green. The leaf color mutant involved in this invention only exhibits albinoness during the seedling stage and does not continuously express this trait throughout the entire growth period, having a minimal impact on maize growth and yield. Applying this InDel molecular marker to maize hybrid seed production and purity identification can achieve rapid and accurate screening of true and false hybrids before sowing or during the seedling stage, significantly improving the purity of hybrids.

The invention discloses an SNP molecular marker combination related to egg quality, a detection reagent and application of the SNP molecular marker combination and the detection reagent, and belongs to the technical field of molecular genetics. According to the invention, analysis is carried out based on large population data of 3388 Wenshui green-shell laying hens, 30 SNP molecular markers significantly related to egg quality are screened, the molecular markers cover multiple dimensions such as egg weight, eggshell quality and internal quality, and the problems of single molecular marker and poor specificity in existing research are solved; the method provides a universal technical framework and an accurate genetic target for the cultivation of a new strain of high-quality laying hens, has a wide industrial application prospect, and is of great significance in promoting the modernization development of the laying hen breeding industry in China.

The application belongs to the technical field of molecular genetics, and particularly relates to a detection method of an insertion / deletion marker of a Shanbei white cashmere goat ASIC2 gene and application thereof. The application uses the designed amplification primer pair, takes the whole genome DNA of the cashmere goat to be detected as a template, amplifies the 5' regulatory region fragment (NC_030826.1:g.16746415_16746441) of the cashmere goat ASIC2 gene through PCR, and then performs agarose gel electrophoresis and sequencing technology to identify the genotype of the insertion / deletion polymorphism site of the cashmere goat ASIC2 gene NC_030826.1:g.16746415_16746441del. The results of the examples show that the different genotypes of the InDel site of the cashmere goat ASIC2 gene are significantly related to the chest depth and cross section height of the Shanbei white cashmere goat, and can be used as an effective DNA marker of the growth traits of the Shanbei white cashmere goat. Further, the DNA marker can assist in breeding excellent cashmere goats.