173 results about "Transcription factor" patented technology

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses application of a rice transcription factor OsWRKY39 and a coding gene thereof in rice blast resistance of rice, and belongs to the technical field of gene engineering. According to the invention, an overexpression vector and a gene knockout vector of the rice OsWRKY39 gene are constructed, the vector is transferred into a Kitaake receptor material, an OsWRKY39 overexpression plant and an OsWRKY39 knockout plant are obtained, and the obtained transgenic plants are subjected to disease resistance analysis. Results show that knockout of the OsWRKY39 gene significantly improves the resistance level of rice to rice blast, which indicates that the OsWRKY39 negatively regulates the disease resistance of plants, and the encoding gene OsWRKY39 can be used as a target gene for improving the disease resistance of plants, has a good application prospect in cultivation of disease-resistant rice varieties, and lays an important foundation for rice resistance breeding.

The invention relates to application of rice OsDREB6 and a coding gene in regulation and control of flumioxazin stress resistance of plants. The application comprises application of a rice transcription factor OsDREB6 and a coding gene thereof in regulation and control of flumioxazin stress resistance of plants and / or improvement of genetic breeding of flumioxazin tolerance of rice. Research finds that the rice transcription factor OsDREB6 participates in regulation and control of stress response of rice to flumioxazin for the first time, compared with wild type rice, rice seedlings overexpressed by OsDREB6 have the root length and bud length obviously larger than those of the wild type rice under the condition of flumioxazin stress, the rice seedlings show very good tolerance to flumioxazin, and the rice transcription factor OsDREB6 has good application prospects in regulation and control of the stress response of rice to flumioxazin. The damage of the flumioxazin to the rice is effectively reduced. Therefore, the rice transcription factor OsDREB6 can be used for improving the flumioxazin stress resistance of the rice and improving the molecular breeding of the flumioxazin resistance of the rice, and is of great significance to the cultivation of flumioxazin-resistant rice germplasm resources.

The invention belongs to the technical field of plant genetic engineering, and relates to a tulip transcription factor TgTCP2 for regulating and controlling sugar transport and growth and development of plants and application of the tulip transcription factor TgTCP2, the plant growth and development traits comprise at least one of seedball size, crown breadth size, rosette leaf number and plant height, the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 1, and the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 2. Or the corresponding amino acid sequence is as shown in SEQ ID NO: 2. The result shows that the overexpression TgTCP2 promotes the growth and development of arabidopsis thaliana, which is mainly reflected in the increase of crown breadth, the increase of rosette leaf number and the increase of plant height; a VIGS technology is utilized to silence TgTCP2, and it is found that tulip seedball development is inhibited by silence of the gene, the sugar content in tulip seedballs is reduced, and it is indicated that TgTCP2 has the effect of regulating tulip seedball development and sugar transport. Application of the gene has important practical significance on regulation and control of plant sugar transport and growth and development.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention discloses a production strain for fermenting L-histidine and application of the production strain, and belongs to the technical field of biological engineering. According to the invention, escherichia coli E.coli CICC 10243 is taken as an original strain, histidine synthesis operon genes hisDCB-cg2302-cg2301-HA-impA-FI, hisN and hisEG from corynebacterium glutamicum are integrated on a genome of the escherichia coli E.coli CICC 10243, a pyruvate dehydrogenase AceE mutant coding gene is introduced, and an escherichia coli transcription factor SlyA coding gene slyA is inactivated. The novel strain E.coli His08 for fermentation production of L-histidine is prepared and fermented in a 5L fermentation tank for 60 h, the histidine yield reaches 58.39 g / L, the sugar-acid conversion rate is 0.15 g / g, and the strain E.coli His08 has important application value.

The invention is suitable for the technical field of molecular biology, and provides a rice transcription factor OsGATA16 and application thereof in regulation and control of rice blast resistance of rice. The method comprises the following steps: firstly, cloning a rice OsGATA16 gene, then constructing an overexpression vector and a mutant vector, and transforming rice to obtain a corresponding strain. Research finds that after a mutant strain with OsGATA16 knocked out is inoculated with germs, the number of scabs is obviously smaller than that of a wild type control group, the disease resistance of rice can be accurately regulated and controlled, and the rice blast resistance is obviously improved. The regulation and control mode can effectively reduce pesticide use and reduce environmental pollution and residual risk. Meanwhile, the successfully constructed stable genetic regulation material can be stored for a long time and used for multiple times in molecular and phenotype levels through verification. Besides, the OsGATA16 gene can be used as a disease-resistant molecular marker or a functional gene to be applied to various breeding systems, a new target is provided for breeding new rice materials with high rice blast resistance, and the OsGATA16 gene has a wide practical application prospect.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention relates to recombinant pichia pastoris capable of efficiently expressing III-type human-like collagen and application of the recombinant pichia pastoris, and belongs to the technical field of microorganisms. According to the invention, an III-type human-like collagen expression cassette is constructed in pichia pastoris GS115 and co-expressed with a molecular chaperone, a transcription factor and a translation factor, so that the expression efficiency of the III-type human-like collagen is improved, and the shake-flask fermentation yield reaches 0.93 g / L. And then fermentation conditions, material supplementing conditions, methanol feeding induction conditions and the like are optimized, so that the yield of the III-type human-like collagen in a 5L fermentation tank by the constructed recombinant pichia pastoris reaches 10.3 g / L, the efficient production of target protein is realized, a favorable tool is provided for the application of the III-type human-like collagen in the fields of food, cosmetics, medicines and the like, and the engineering application prospect is broad. Good industrial application prospects are realized.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention discloses application of a tea tree CsNAC25 gene in negative regulation of cold resistance of plants, the gene belongs to a tea tree NAC transcription factor family, and the nucleotide sequence of the gene is shown as SEQ ID NO.1. The tea tree CsNAC25 gene negatively regulates the cold resistance of the tea tree, and the gene expression is reduced under low temperature induction. The over-expression of the gene in tobacco weakens the cold resistance of a transgenic plant, and the expression of CsNAC25 is silenced by adopting a VIGS technology in a tea tree, so that the cold resistance of the tea tree is remarkably improved. Silencing CsNAC25 effectively reduces the wilting degree and the ion leakage rate of tea seedling leaves under low temperature stress, activates a key CBF signal channel and promotes accumulation of osmoregulation substances. The application of the application is expected to effectively improve the cold resistance when low-temperature disasters such as late spring coldness occur, and has important theoretical significance and wide application prospects for guaranteeing the stable yield and quality of the tea industry.

The invention provides an application of a ganoderma lucidum transcription factor yabby1 in regulating and controlling the transcriptional activity of a ganoderic acid synthetic gene SQS and a method, and belongs to the field of gene engineering. The nucleotide sequence of the yabby1 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the yabby1 gene is as shown in SEQ ID NO. 2; the ganoderma lucidum transcription factor yabby1 gene and the ganoderma lucidum SQS gene are constructed on a recombinant vector and are introduced into cells, so that the expression of the ganoderma lucidum SQS gene can be activated, and the content of ganoderic acid in ganoderma lucidum can be increased.

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The invention discloses application of Bombyx mori neuropeptide BommoOKAtype4 in preparation of a medicine for inhibiting melanoma cell growth, and belongs to the technical field of medical biology. Aiming at the technical problems that melanoma is high in malignancy degree, easy to transfer and limited in treatment means, and whether the bombyx mori neuropeptide BommoOKAtype4 can directly inhibit the growth of melanoma cells is not clear yet when the bombyx mori neuropeptide BommoOKAtype4 is mainly used for skin whitening before, the invention provides the application of the bombyx mori neuropeptide in preparing the medicine for inhibiting the melanoma cells. According to the silkworm neuropeptide, gene expression and protein functions of melanin synthesis key enzymes TYR, TRP1 and TRP2 are comprehensively inhibited by down-regulating expression of a core transcription factor MITF, so that melanoma cell proliferation is directly inhibited while melanin synthesis is inhibited, and therefore, the silkworm neuropeptide can be used for preparing drugs for treating melanoma and has a wide application prospect. A novel peptide candidate substance is provided for treatment of melanoma.

To provide novel methods and uses for increasing a yield of recombinant proteins in host cells, which are simple, efficient and suitable for use in industrial methods.SOLUTION: Provided is a method of increasing a yield of a protein of interest (POI) in an eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4 / 2. Further provided are a recombinant eukaryotic host cell for manufacturing the POI, the host cell being engineered to overexpress at least one polynucleotide encoding at least one transcription factor, as well as the use of the host cell for manufacturing the POI.SELECTED DRAWING: None

The invention discloses liver-responsive synthetic macrophages and a construction method and tumor immunotherapy application thereof, and belongs to the technical field of biological medicines. The system is an IBMDM cell line of a stable expression alpha SLC17A2-P65 / shSIRP alpha-synM system, and comprises an extracellular domain and an intracellular domain, the extracellular domain is composed of a single-chain antibody scFv for recognizing an antigen SLC17A2 and a transcription factor GAL4-VP16 (GV), and the intracellular domain is composed of a UAS promoter sequence recognized by the GV and P65 / shSIRP alpha. When the antibody responds to the liver, GV is released through the self-cleavage effect of synNotch, and the GV recognizes a UAS promoter sequence after entering a nucleus to activate downstream gene expression. The invention also provides a construction method of the system. The construction method comprises the following three steps: constructing a vector plasmid, packaging lentivirus and constructing a target cell line. The system can be applied to screening anti-liver cancer immunotherapy drugs, and a new research tool is provided for liver cancer treatment.

The present invention relates to inhibitors of NFkappaB activity for the treatment of diseases and disorders, and discloses peptides and methods of use thereof for the treatment of various diseases and disorders, including inflammation, pain, oral mucositis, oral lesions, and cancer. The peptides modulate the activity of the transcription factor NFkappaB.

The present invention belongs to the field of genetic engineering technology and discloses a transcription factor RsRAP2-12 for regulating radish anthocyanin synthesis and its application. The nucleotide sequence of the RsRAP2-12 gene is such as SEQ ID No. 1, which can be used to regulate the synthesis of radish anthocyanins. The present invention also obtains an overexpression vector and a VIGS silencing vector for the RsRAP2-12 gene; and proves that transient overexpression of RsRAP2-12 on radish leaves can increase the anthocyanin content on the leaves, and injecting the constructed VIGS silencing vector into the roots of red-skinned, red-fleshed radishes can reduce the accumulation of anthocyanins. Therefore, the RsRAP2-12 gene plays an important role in regulating the synthesis of radish anthocyanins. The present invention is of great significance for breeding new radish varieties with high anthocyanin content and provides strong technical support for improving radish quality.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The present application relates to a kind of rice transcription factor OsLSD5 and its application in anti-rice stripe virus, by the overexpression OsLSD5 vector after being transferred in plant rice, obtain the stable genetic OsLSD5-ox transgenic plant.

The invention provides a nucleic acid construct which comprises an immune response regulatory sequence and a coding sequence of a reporter gene driven by a promoter sequence, and the immune response regulatory sequence comprises a transcription factor binding regulatory element RE which is co-stimulated and regulated by transcription factors AP-1 and CD28. The invention also provides an in-vitro immune effector function reporter gene cell model containing the nucleic acid construct, and the cell model can realize effector function activity determination performance of sensitive and potent signals so as to evaluate ADCC and ADCP killing action mechanisms and titers mediated by antibody-dependent disease cells designed by an Fc structural domain of a therapeutic antibody product. In addition, the immunosuppression and regulation efficacy of a targeted CTLA-4 or Abatacept mediated treatment method on a CD28 co-activation pathway can be evaluated.

The invention relates to the application of inhibition of transcription factor 4 (TCF4 / ITF2) in the treatment of cancer. This in particular in combination therapy in conjunction with an immunotherapeutic compound (such as for treating cancers poorly responding or refractive to immunotherapy). In particular, inhibition of TCF4 / ITF2 is capable of restoring response to immunotherapy such as immune checkpoint inhibitor therapy. Expression levels of TCF4 / ITF2 as well as levels of mesenchymal-like cancer cells in a cancer lesion are predictive of future response to immunotherapy early after initiation of the immunotherapy. Further part of the invention are methods of detecting mesenchymal-like cancer cells.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

T lymphocytes play a key role in the immune response and their functions are intimately linked to metabolic programs. During immune responses, T cells undergo a metabolic reprogramming notably characterized by an increased aerobic glycolysis. Using a quantitative phosphoproteomic approach, the inventors have identified a new transcription factor called Foxk1 as being highly phosphorylated in T cells upon T Cell Receptor (TCR) engagement. The results also indicate that Foxk1 phosphorylation and nuclear translocation is dependent of the AKT-mTOR kinase activities. Using T-cell specific Foxk1 deficient mice (Foxk1- / -), we demonstrated that Foxk1 is required for full T cell activation. Foxk1-deficient T cells exhibited reduced proliferation and cytokine secretion following TCR stimulation. Furthermore, T cells from Foxk1- / - mice were less prone to acquire an effector like phenotype than wild-type cells when challenged in vivo. Conversely, Foxk1 overexpression in T cells enhanced their effector functions in a TCR-dependent manner. In CD8+ T cells, this effect also results into enhanced cancer cell killing capacity in vitro, and improved tumor rejection in vivo. Altogether, these results indicated that Foxk1 is a major regulator of T cell metabolism and thus, of T cell effector functions. Thus, the present invention relates to host immune cells engineered to overexpress a Foxk1 polypeptide and their use of the treatment of cancer.

The invention discloses a saccharomyces cerevisiae pyruvic acid response biosensor as well as a construction method and application thereof. The biosensor comprises a pyruvic acid activation type gene loop or a pyruvic acid inhibition type gene loop or a bifunctional gene loop. According to the invention, a pyruvic acid activation type gene loop is constructed in saccharomyces cerevisiae through pyruvic acid transcription factor PdhR structural design and PdhR expression level optimization, and the dynamic multiple is 3.4. According to the method, a pyruvic acid activated promoter is used for inducing XylR expression by utilizing the performance of a xylose transcription inhibition factor XylR inversion gene loop, so that transcription of a promoter embedded with an XylR binding site is inhibited, a pyruvic acid inhibition type gene loop is constructed, and the dynamic multiple is 3.1 times. And a difunctional pyruvic acid gene loop with orthogonality is further constructed. The pyruvic acid response loop constructed by the invention takes glucose as an activating agent, and can more widely regulate and control the complex metabolic pathway of the saccharomyces cerevisiae.

The present invention discloses the application of the rice transcription factor OsbHLH091 and its encoding gene in disease-resistant breeding, belonging to the field of genetic engineering. The amino acid sequence of the rice transcription factor OsbHLH091 is shown in SEQ ID NO.2, and the encoding gene is shown in SEQ ID NO.1. The present invention proves through experiments that the encoding gene of the rice transcription factor OsbHLH091 is overexpressed in rice, and the transgenic rice plants have higher disease resistance than the recipient rice plants and the rice plants with OsbHLH091 knocked out, indicating that OsbHLH091 positively regulates rice resistance to rice blast. The OsbHLH091 protein and its encoding gene discovered by the present invention are of great significance for breeding disease-resistant rice varieties and can be applied to molecular design to breed rice with improved disease resistance.

The MYC and NMYC transcription factors (TFs) play a key role in cell proliferation and are overexpressed in most cancer cells. However, in normal cells their overexpression triggers safeguard mechanisms promoting cell death and cellular senescence, which are bypassed in cancer cells. Here, the inventors reveal that in normal cells MYC binds to the Inositol 1,4,5-Trisphosphate Receptor type 1 (ITPR1) gene and upregulates its expression, triggering an ER-mitochondria calcium (Ca2+) transfer, which is involved in MYC-induced cell death and senescence. Supporting a tumor suppressive role of MYC / ITPR1 axis, ITPR1 expression is generally decreased in cancer and reactivation of this pathway induces cancer cell death. Nevertheless, some cancer cells, generally expressing high levels of MYCN and / or MYC, also express high level of ITPR1, which correlates with high expression of BCL2, encoding an inhibitor of ITPR1. Strikingly, in high-risk MYCN-amplified neuroblastoma, ITPR1 expression is controlled by NMYC and its level correlates with worse patient survival. In these cells, blocking the interaction between BCL2 and ITPR1, via an BCL2-BH4 domain inhibitor induces mitochondrial Ca2+ accumulation and cell death, and decreases tumor size. Thus, the present invention relates to a method for treating MYChigh cancer, and in particular NMYC--amplified neuroblastoma in a subject by administering an BCL2-BH4 domain inhibitor.

The invention provides a biosensor for accumulating pyruvic acid as well as a screening method and application thereof, the biosensor comprises a positive biosensor and a negative biosensor, the positive biosensor is formed by fusing an activation transcription factor binding site of a Cra gene as shown in SEQ ID NO: 113 at the downstream of a Cra regulation promoter sequent-10 region, and the negative biosensor is formed by fusing an activation transcription factor binding site of a Cra gene as shown in SEQ ID NO: 113 at the downstream of a Cra regulation promoter sequent-10 region; the negative biosensor is formed by fusing an inhibitory transcription factor binding site of a Cra gene as shown in SEQ ID NO: 114 at the downstream of a Cra regulation promoter sequent-10 region. The biosensor can be used for screening or constructing dominant chassis bacteria for accumulating branched chain amino acids including L-isoleucine, and provides powerful support for chassis bacteria required by subsequent high-yield L-isoleucine gene engineering modification.

This invention provides a novel screening method for signaling pathway activators and inhibitors based on cell viability / death phenotypes. Specifically, this invention provides genetically engineered cells that enable large-scale screening of signaling pathway activators and inhibitors based on the expression of protective and suicide genes driven by signaling pathway transcription factors. The viability / death phenotype reporter system is effective and convenient for screening drugs affecting signaling pathways and can be applied to high-throughput screening in the early stages of drug development.