298 results about "Transcription factor" patented technology

Disclosed herein are compositions and methods for modulating T cells. For example, the compositions and methods may be used to increase memory T cells. The compositions and methods may increase the expression or protein level of a transcription factor selected from TGIF2LX, TGIF1, TGIF2, FOS, HNF4A, KLF8, NFKBIZ, CARF, EBF3, HMX3, LHX4, LMX1A, PLAG1, PLAGL1, POU2F3, SOX14, TFAP2D, and WT1, or a combination thereof. The compositions and method may be used in combination with Adoptive T Cell Therapy (ACT) to enhance the ACT.

The invention discloses a CsbHLH118 gene and application of the CsbHLH118 gene in regulation of citrus sour rot, and belongs to the technical field of gene engineering. The nucleotide sequence of the transcription factor CsbHLH118 is shown as SEQ ID NO.1. The transcription factor CsbHLH118 provided by the invention has the advantages that the occurrence of the sour rot of the navel oranges can be delayed by transient overexpression of the CsbHLH118, the resistance to sour rot bacteria is lost by transient silence of the CsbHLH118, and the CsbHLH118 plays a positive regulation role in the resistance to the postharvest sour rot of the navel oranges. The invention provides a target gene for developing effective bactericides.

The invention relates to a transcription factor OsMYB4P gene and application of the transcription factor OsMYB4P gene in antiviral rice breeding.

The invention belongs to the technical field of gene engineering, and particularly relates to a CaNAC02 gene for regulating and controlling synthesis of carotenoid in pepper fruits and application, and the amino acid sequence of protein encoded by the CaNAC02 gene is as shown in SEQ ID NO.1. The CaNAC02 gene disclosed by the invention has the effects of promoting synthesis of carotenoid compounds, influencing quality formation of the pepper fruits and the like in the pepper fruits. The CaNAC02 gene provided by the invention is an NAC family transcription factor, can provide a target gene for cultivation of a new pepper variety, and provides a genetic material and a theoretical basis for genetic improvement of the pepper variety.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses a gene DobHLH14 for regulating and controlling DoSUS2 expression under drought stress to increase the content of dendrobium officinale polysaccharides and application of the gene DobHLH14, and belongs to the technical field of plant genetic engineering breeding, the nucleotide sequence of the gene DobHLH14 is shown as SEQ ID No: 4, and the coded amino acid sequence is shown as SEQ ID No: 5. The DobHLH14 is applied to the improvement of the polysaccharide content of the plant or the breeding of the drought-tolerant transgenic plant. The homologous and heterologous overexpression results of the dendrobium officinale show that the polysaccharide content and drought resistance of the transgenic plant are obviously improved by regulating the expression of the DoSUS2 gene through the DobHLH14 transcription factor of the dendrobium officinale under drought stress, and the method is an excellent method for breeding perennial herbaceous plants with high polysaccharide and drought resistance.

The present invention relates to compositions and methods for modifying an elongated hypocotyl 5 (HY5) transcription factor in a plant to inhibit shading responses. The invention also relates to plants and plant parts produced using the methods and compositions of the invention.

The invention discloses application of a rice transcription factor OsWRKY39 and a coding gene thereof in rice blast resistance of rice, and belongs to the technical field of gene engineering. According to the invention, an overexpression vector and a gene knockout vector of the rice OsWRKY39 gene are constructed, the vector is transferred into a Kitaake receptor material, an OsWRKY39 overexpression plant and an OsWRKY39 knockout plant are obtained, and the obtained transgenic plants are subjected to disease resistance analysis. Results show that knockout of the OsWRKY39 gene significantly improves the resistance level of rice to rice blast, which indicates that the OsWRKY39 negatively regulates the disease resistance of plants, and the encoding gene OsWRKY39 can be used as a target gene for improving the disease resistance of plants, has a good application prospect in cultivation of disease-resistant rice varieties, and lays an important foundation for rice resistance breeding.

The invention relates to application of rice OsDREB6 and a coding gene in regulation and control of flumioxazin stress resistance of plants. The application comprises application of a rice transcription factor OsDREB6 and a coding gene thereof in regulation and control of flumioxazin stress resistance of plants and / or improvement of genetic breeding of flumioxazin tolerance of rice. Research finds that the rice transcription factor OsDREB6 participates in regulation and control of stress response of rice to flumioxazin for the first time, compared with wild type rice, rice seedlings overexpressed by OsDREB6 have the root length and bud length obviously larger than those of the wild type rice under the condition of flumioxazin stress, the rice seedlings show very good tolerance to flumioxazin, and the rice transcription factor OsDREB6 has good application prospects in regulation and control of the stress response of rice to flumioxazin. The damage of the flumioxazin to the rice is effectively reduced. Therefore, the rice transcription factor OsDREB6 can be used for improving the flumioxazin stress resistance of the rice and improving the molecular breeding of the flumioxazin resistance of the rice, and is of great significance to the cultivation of flumioxazin-resistant rice germplasm resources.

The invention discloses a CsMUTE gene and application thereof in regulation of citrus sour rot, and belongs to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. The invention provides a transcription factor CsMUTE, and experiments find that transient overexpression of CsMUTE can delay the occurrence of sour rot of navel oranges, transient silence of CsMUTE loses resistance to sour rot bacteria, and CsMUTE plays a positive regulation role in resistance of postharvest sour rot of navel oranges. The invention provides a target gene for developing effective bactericides.

The invention discloses application of a rice transcription factor bZIP65 in improving the nitrogen utilization efficiency of plants, and relates to the technical field of biology. The construction of an overexpression plant of the bZIP65 gene proves that the overexpression of the gene can promote the transport and absorption of nitrate by the rice and improve the nitrogen fertilizer utilization rate, and the genetic mechanism of the nitrogen utilization rate of the rice is further clarified through the function interpretation of the bZIP65 gene. Meanwhile, a theoretical basis and a technical support can be provided for explaining and improving the nitrogen utilization efficiency of the rice and genetic improvement of varieties of the rice.

The invention belongs to the technical field of plant genetic engineering, and relates to a tulip transcription factor TgTCP2 for regulating and controlling sugar transport and growth and development of plants and application of the tulip transcription factor TgTCP2, the plant growth and development traits comprise at least one of seedball size, crown breadth size, rosette leaf number and plant height, the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 1, and the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 2. Or the corresponding amino acid sequence is as shown in SEQ ID NO: 2. The result shows that the overexpression TgTCP2 promotes the growth and development of arabidopsis thaliana, which is mainly reflected in the increase of crown breadth, the increase of rosette leaf number and the increase of plant height; a VIGS technology is utilized to silence TgTCP2, and it is found that tulip seedball development is inhibited by silence of the gene, the sugar content in tulip seedballs is reduced, and it is indicated that TgTCP2 has the effect of regulating tulip seedball development and sugar transport. Application of the gene has important practical significance on regulation and control of plant sugar transport and growth and development.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention discloses a schizophrenia risk structure variation identification and function evaluation method based on three-generation sequencing, and relates to the field of molecular biology, whole genome sequencing is performed on peripheral blood DNA of a patient through three-generation sequencing, multi-tool joint detection is adopted, multi-sample results are integrated, and a high-confidence SV data set is generated; through cross-queue comparison, the patient specific SV is screened, and the high-risk potential pathogenic SV is identified in combination with an SV priority ordering tool SVJudge. By combining transcription factor binding analysis, SCZ drug target data and histocyte specific expression data, the influence of SV on gene regulation is evaluated, and the potential action mechanism of SV in SCZ is analyzed. The SCZ risk gene is screened based on SVJudge scoring and patient carrying conditions, the genetic risk and pathogenic mechanism of the SCZ are analyzed and verified through pathway enrichment, a protein interaction network and a functional module, and a new technical means and theoretical basis are provided for genetic research of the SCZ.

The invention relates to a high-temperature-resistant saccharomyces cerevisiae strain and a construction method and application thereof in the field of microorganisms. The high-temperature-resistant saccharomyces cerevisiae strain disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.27605, and the preservation name is SEB22. The SEB22 bacterial strain is obtained by knocking out an L-asparaginase II coding gene ASP3 and a high-expression transcription factor CRZ1 from an original bacterial strain SEB4. The glucose consumption rate, the ethanol yield and the yield of the SEB22 strain under high-temperature stress are obviously improved, and the SEB22 strain has good application potential in the aspect of high-temperature SSF fermentation.

The invention discloses a production strain for fermenting L-histidine and application of the production strain, and belongs to the technical field of biological engineering. According to the invention, escherichia coli E.coli CICC 10243 is taken as an original strain, histidine synthesis operon genes hisDCB-cg2302-cg2301-HA-impA-FI, hisN and hisEG from corynebacterium glutamicum are integrated on a genome of the escherichia coli E.coli CICC 10243, a pyruvate dehydrogenase AceE mutant coding gene is introduced, and an escherichia coli transcription factor SlyA coding gene slyA is inactivated. The novel strain E.coli His08 for fermentation production of L-histidine is prepared and fermented in a 5L fermentation tank for 60 h, the histidine yield reaches 58.39 g / L, the sugar-acid conversion rate is 0.15 g / g, and the strain E.coli His08 has important application value.

The invention provides a method for inducing synthesis of capsaicin by using an optical signal transcription factor CaBBX2, and particularly relates to an experimental method for the influence of a living plant silencing CaBBX2 gene on synthesis of capsaicin. Capsicum fruits after pollination for 16 days, 25 days, 33 days and 38 days are taken to observe and analyze the content of capsaicin, dihydrocapsaicin and total capsaicin and the change condition of capsaicin synthesis related gene expression, and experimental results show that after the CaBBX2 gene is silenced, the content of capsaicin is remarkably reduced relative to that of a no-load control group, which indicates that the CaBBX2 and the biosynthesis of capsaicin have a certain correlation, and along with the silencing of the CaBBX2, the content of capsaicin is remarkably reduced relative to that of a no-load control group. The expression quantity of the capsaicin synthesis related gene is also reduced to different degrees, and further verification shows that the CaBBX2 can influence the synthesis of capsaicin by regulating the expression of the capsaicin synthesis related gene.

The invention is suitable for the technical field of molecular biology, and provides a rice transcription factor OsGATA16 and application thereof in regulation and control of rice blast resistance of rice. The method comprises the following steps: firstly, cloning a rice OsGATA16 gene, then constructing an overexpression vector and a mutant vector, and transforming rice to obtain a corresponding strain. Research finds that after a mutant strain with OsGATA16 knocked out is inoculated with germs, the number of scabs is obviously smaller than that of a wild type control group, the disease resistance of rice can be accurately regulated and controlled, and the rice blast resistance is obviously improved. The regulation and control mode can effectively reduce pesticide use and reduce environmental pollution and residual risk. Meanwhile, the successfully constructed stable genetic regulation material can be stored for a long time and used for multiple times in molecular and phenotype levels through verification. Besides, the OsGATA16 gene can be used as a disease-resistant molecular marker or a functional gene to be applied to various breeding systems, a new target is provided for breeding new rice materials with high rice blast resistance, and the OsGATA16 gene has a wide practical application prospect.

The invention belongs to the technical field of plant biology, and provides a method for regulating and controlling the heading stage of rice by using an OsTCP2 gene, and the OsTCP2 is used as a TCP family transcription factor and plays an important role in regulating and controlling the heading stage of the rice. Experimental results show that the heading period of the OsTCP2 function deletion mutant strain is obviously advanced. The nucleotide sequence of the OsTCP2 gene is defined, the mutant of the OsTCP2 gene is constructed, and an OsTCP2 mutant plant is successfully obtained through a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) technology. The mutants show an obvious early blossoming phenomenon under a long-day condition, and the heading period is about 8 days earlier than that of a wild type on average. A new gene resource is provided for regulation and control of the heading period of the rice, rice varieties can be improved through a molecular breeding means, the adaptive capacity and yield of the rice varieties in different regions are improved, and the gene has important significance on agricultural production.

The invention discloses a larimichthys crocea IL-22 recombinant protein which is a larimichthys crocea IL-22 mature peptide sequence, and the amino acid sequence of the larimichthys crocea IL-22 recombinant protein is as shown in SEQ ID NO. 2. The recombinant protein is obtained by amplifying and coding gene segments of amino acids from 31st to 190th positions of large yellow croaker IL-22 mature peptide through PCR (Polymerase Chain Reaction), and transforming escherichia coli for heterologous expression. The larimichthys crocea IL-22 recombinant protein can activate a transcription factor STAT3 in a larimichthys crocea head kidney cell line (LYCK) and also can induce expression of inflammatory factors (IL-1beta, IL-6 and IL-8) in immune organs of larimichthys crocea, so that the larimichthys crocea IL-22 recombinant protein has an obvious immunoregulation effect and shows a good application prospect in research and development of aquatic immunomodulators.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention relates to recombinant pichia pastoris capable of efficiently expressing III-type human-like collagen and application of the recombinant pichia pastoris, and belongs to the technical field of microorganisms. According to the invention, an III-type human-like collagen expression cassette is constructed in pichia pastoris GS115 and co-expressed with a molecular chaperone, a transcription factor and a translation factor, so that the expression efficiency of the III-type human-like collagen is improved, and the shake-flask fermentation yield reaches 0.93 g / L. And then fermentation conditions, material supplementing conditions, methanol feeding induction conditions and the like are optimized, so that the yield of the III-type human-like collagen in a 5L fermentation tank by the constructed recombinant pichia pastoris reaches 10.3 g / L, the efficient production of target protein is realized, a favorable tool is provided for the application of the III-type human-like collagen in the fields of food, cosmetics, medicines and the like, and the engineering application prospect is broad. Good industrial application prospects are realized.

Described herein are methods of identifying interacting components of a protein of interest. Also described herein are fusion proteins that include a protein of interest or a binding-agent that binds a protein of interest and a labeling enzyme.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention discloses application of a tea tree CsNAC25 gene in negative regulation of cold resistance of plants, the gene belongs to a tea tree NAC transcription factor family, and the nucleotide sequence of the gene is shown as SEQ ID NO.1. The tea tree CsNAC25 gene negatively regulates the cold resistance of the tea tree, and the gene expression is reduced under low temperature induction. The over-expression of the gene in tobacco weakens the cold resistance of a transgenic plant, and the expression of CsNAC25 is silenced by adopting a VIGS technology in a tea tree, so that the cold resistance of the tea tree is remarkably improved. Silencing CsNAC25 effectively reduces the wilting degree and the ion leakage rate of tea seedling leaves under low temperature stress, activates a key CBF signal channel and promotes accumulation of osmoregulation substances. The application of the application is expected to effectively improve the cold resistance when low-temperature disasters such as late spring coldness occur, and has important theoretical significance and wide application prospects for guaranteeing the stable yield and quality of the tea industry.

Disclosed herein are compositions comprising LNPs capable of selectively targeting cancer cells in organs for the efficient delivery of RNA cargos, specifically aimed at targeting poorly druggable, disease-driving transcription factors in prostate cancer and methods of use thereof.

The invention relates to the technical field of biological medicines, and particularly discloses a transcription factor HOMEZ phosphorylation site specific antibody as well as a preparation method and application thereof. The specific antibody provided by the invention can specifically recognize phosphorylation of the 451st site threonine of HOMEZ in a tumor specimen, so that the prognosis condition of the tumor is judged, and a potential action target is provided for clinical diagnosis and treatment of the tumor; moreover, the specific antibody disclosed by the invention can be used for detecting the expression difference between normal cells and tumor cells in a Western blot experiment, and is beneficial to research on the effect of phosphorylation modification of HOMEZ protein in the occurrence and development process of tumor diseases; and the kit can also be used for detecting the phosphorylation level of the HOMEZ protein in a clinical specimen through experiments such as immunofluorescence and immunohistochemical staining, and has a wide clinical application prospect in the aspects of disease diagnosis, treatment, prognosis judgment and the like.

Disclosed herein are methods of gene editing, or endogenous suppression, of cytokines / chemokines / transcription factors secreted from chimeric antigen receptor (CAR)-bearing immune effector cell such as CAR-T cells for the mitigation of cytokine release syndrome and / or CAR-T associated neuropathy. These methods involve insertion of the CAR into a locus of a cytokine gene, blocking its expression. Also disclosed herein are (CAR)-bearing immune effector cells with CARs inserted into a locus of a cytokine gene, and methods of treatment of diseases with immunotherapy with a reduced incidence of cytokine release syndrome and / or CAR-T associated neuropathy.

The invention provides an application of a ganoderma lucidum transcription factor yabby1 in regulating and controlling the transcriptional activity of a ganoderic acid synthetic gene SQS and a method, and belongs to the field of gene engineering. The nucleotide sequence of the yabby1 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the yabby1 gene is as shown in SEQ ID NO. 2; the ganoderma lucidum transcription factor yabby1 gene and the ganoderma lucidum SQS gene are constructed on a recombinant vector and are introduced into cells, so that the expression of the ganoderma lucidum SQS gene can be activated, and the content of ganoderic acid in ganoderma lucidum can be increased.

The invention provides a gene regulation network inference method based on second-order multi-channel directed graph convolution, and belongs to the technical fields of graph representation learning, system biology and the like. Firstly, a first-order proximity matrix, a second-order in-degree proximity matrix and a second-order out-degree proximity matrix are constructed respectively, an existing method is expanded through the second-order proximity matrix, secondly, feature information implied in the three matrixes and expression data is extracted by using multiple graph convolution networks respectively, and the feature information of the three matrixes and the expression data is extracted. And then fusion operation is carried out to fuse features extracted by the multi-channel directed graph convolutional network. And finally, respectively extracting embedding expressions of transcription factors and target genes in potential regulatory gene pairs by using two channels, and obtaining a predicted regulatory probability through dot product operation. According to the method, the prior regulation and control information and the directional regulation and control information implied in the expression data are extracted, and the perception domain of the graph convolution is expanded from the first order to the second order, so that richer feature information can be extracted, and the inference accuracy of the potential directional regulation and control relationship is improved.