230 results about "Transcription factor" patented technology

The invention relates to a transcription factor OsMYB4P gene and application of the transcription factor OsMYB4P gene in antiviral rice breeding.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses application of a rice transcription factor OsWRKY39 and a coding gene thereof in rice blast resistance of rice, and belongs to the technical field of gene engineering. According to the invention, an overexpression vector and a gene knockout vector of the rice OsWRKY39 gene are constructed, the vector is transferred into a Kitaake receptor material, an OsWRKY39 overexpression plant and an OsWRKY39 knockout plant are obtained, and the obtained transgenic plants are subjected to disease resistance analysis. Results show that knockout of the OsWRKY39 gene significantly improves the resistance level of rice to rice blast, which indicates that the OsWRKY39 negatively regulates the disease resistance of plants, and the encoding gene OsWRKY39 can be used as a target gene for improving the disease resistance of plants, has a good application prospect in cultivation of disease-resistant rice varieties, and lays an important foundation for rice resistance breeding.

The invention relates to application of rice OsDREB6 and a coding gene in regulation and control of flumioxazin stress resistance of plants. The application comprises application of a rice transcription factor OsDREB6 and a coding gene thereof in regulation and control of flumioxazin stress resistance of plants and / or improvement of genetic breeding of flumioxazin tolerance of rice. Research finds that the rice transcription factor OsDREB6 participates in regulation and control of stress response of rice to flumioxazin for the first time, compared with wild type rice, rice seedlings overexpressed by OsDREB6 have the root length and bud length obviously larger than those of the wild type rice under the condition of flumioxazin stress, the rice seedlings show very good tolerance to flumioxazin, and the rice transcription factor OsDREB6 has good application prospects in regulation and control of the stress response of rice to flumioxazin. The damage of the flumioxazin to the rice is effectively reduced. Therefore, the rice transcription factor OsDREB6 can be used for improving the flumioxazin stress resistance of the rice and improving the molecular breeding of the flumioxazin resistance of the rice, and is of great significance to the cultivation of flumioxazin-resistant rice germplasm resources.

The invention belongs to the technical field of plant genetic engineering, and relates to a tulip transcription factor TgTCP2 for regulating and controlling sugar transport and growth and development of plants and application of the tulip transcription factor TgTCP2, the plant growth and development traits comprise at least one of seedball size, crown breadth size, rosette leaf number and plant height, the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 1, and the nucleotide sequence of the TgTCP2 gene is shown as SEQ ID NO: 2. Or the corresponding amino acid sequence is as shown in SEQ ID NO: 2. The result shows that the overexpression TgTCP2 promotes the growth and development of arabidopsis thaliana, which is mainly reflected in the increase of crown breadth, the increase of rosette leaf number and the increase of plant height; a VIGS technology is utilized to silence TgTCP2, and it is found that tulip seedball development is inhibited by silence of the gene, the sugar content in tulip seedballs is reduced, and it is indicated that TgTCP2 has the effect of regulating tulip seedball development and sugar transport. Application of the gene has important practical significance on regulation and control of plant sugar transport and growth and development.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention discloses a schizophrenia risk structure variation identification and function evaluation method based on three-generation sequencing, and relates to the field of molecular biology, whole genome sequencing is performed on peripheral blood DNA of a patient through three-generation sequencing, multi-tool joint detection is adopted, multi-sample results are integrated, and a high-confidence SV data set is generated; through cross-queue comparison, the patient specific SV is screened, and the high-risk potential pathogenic SV is identified in combination with an SV priority ordering tool SVJudge. By combining transcription factor binding analysis, SCZ drug target data and histocyte specific expression data, the influence of SV on gene regulation is evaluated, and the potential action mechanism of SV in SCZ is analyzed. The SCZ risk gene is screened based on SVJudge scoring and patient carrying conditions, the genetic risk and pathogenic mechanism of the SCZ are analyzed and verified through pathway enrichment, a protein interaction network and a functional module, and a new technical means and theoretical basis are provided for genetic research of the SCZ.

The invention discloses a production strain for fermenting L-histidine and application of the production strain, and belongs to the technical field of biological engineering. According to the invention, escherichia coli E.coli CICC 10243 is taken as an original strain, histidine synthesis operon genes hisDCB-cg2302-cg2301-HA-impA-FI, hisN and hisEG from corynebacterium glutamicum are integrated on a genome of the escherichia coli E.coli CICC 10243, a pyruvate dehydrogenase AceE mutant coding gene is introduced, and an escherichia coli transcription factor SlyA coding gene slyA is inactivated. The novel strain E.coli His08 for fermentation production of L-histidine is prepared and fermented in a 5L fermentation tank for 60 h, the histidine yield reaches 58.39 g / L, the sugar-acid conversion rate is 0.15 g / g, and the strain E.coli His08 has important application value.

The invention is suitable for the technical field of molecular biology, and provides a rice transcription factor OsGATA16 and application thereof in regulation and control of rice blast resistance of rice. The method comprises the following steps: firstly, cloning a rice OsGATA16 gene, then constructing an overexpression vector and a mutant vector, and transforming rice to obtain a corresponding strain. Research finds that after a mutant strain with OsGATA16 knocked out is inoculated with germs, the number of scabs is obviously smaller than that of a wild type control group, the disease resistance of rice can be accurately regulated and controlled, and the rice blast resistance is obviously improved. The regulation and control mode can effectively reduce pesticide use and reduce environmental pollution and residual risk. Meanwhile, the successfully constructed stable genetic regulation material can be stored for a long time and used for multiple times in molecular and phenotype levels through verification. Besides, the OsGATA16 gene can be used as a disease-resistant molecular marker or a functional gene to be applied to various breeding systems, a new target is provided for breeding new rice materials with high rice blast resistance, and the OsGATA16 gene has a wide practical application prospect.

The invention belongs to the technical field of plant biology, and provides a method for regulating and controlling the heading stage of rice by using an OsTCP2 gene, and the OsTCP2 is used as a TCP family transcription factor and plays an important role in regulating and controlling the heading stage of the rice. Experimental results show that the heading period of the OsTCP2 function deletion mutant strain is obviously advanced. The nucleotide sequence of the OsTCP2 gene is defined, the mutant of the OsTCP2 gene is constructed, and an OsTCP2 mutant plant is successfully obtained through a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated 9) technology. The mutants show an obvious early blossoming phenomenon under a long-day condition, and the heading period is about 8 days earlier than that of a wild type on average. A new gene resource is provided for regulation and control of the heading period of the rice, rice varieties can be improved through a molecular breeding means, the adaptive capacity and yield of the rice varieties in different regions are improved, and the gene has important significance on agricultural production.

The invention discloses a larimichthys crocea IL-22 recombinant protein which is a larimichthys crocea IL-22 mature peptide sequence, and the amino acid sequence of the larimichthys crocea IL-22 recombinant protein is as shown in SEQ ID NO. 2. The recombinant protein is obtained by amplifying and coding gene segments of amino acids from 31st to 190th positions of large yellow croaker IL-22 mature peptide through PCR (Polymerase Chain Reaction), and transforming escherichia coli for heterologous expression. The larimichthys crocea IL-22 recombinant protein can activate a transcription factor STAT3 in a larimichthys crocea head kidney cell line (LYCK) and also can induce expression of inflammatory factors (IL-1beta, IL-6 and IL-8) in immune organs of larimichthys crocea, so that the larimichthys crocea IL-22 recombinant protein has an obvious immunoregulation effect and shows a good application prospect in research and development of aquatic immunomodulators.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention relates to recombinant pichia pastoris capable of efficiently expressing III-type human-like collagen and application of the recombinant pichia pastoris, and belongs to the technical field of microorganisms. According to the invention, an III-type human-like collagen expression cassette is constructed in pichia pastoris GS115 and co-expressed with a molecular chaperone, a transcription factor and a translation factor, so that the expression efficiency of the III-type human-like collagen is improved, and the shake-flask fermentation yield reaches 0.93 g / L. And then fermentation conditions, material supplementing conditions, methanol feeding induction conditions and the like are optimized, so that the yield of the III-type human-like collagen in a 5L fermentation tank by the constructed recombinant pichia pastoris reaches 10.3 g / L, the efficient production of target protein is realized, a favorable tool is provided for the application of the III-type human-like collagen in the fields of food, cosmetics, medicines and the like, and the engineering application prospect is broad. Good industrial application prospects are realized.

Described herein are methods of identifying interacting components of a protein of interest. Also described herein are fusion proteins that include a protein of interest or a binding-agent that binds a protein of interest and a labeling enzyme.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention discloses application of a tea tree CsNAC25 gene in negative regulation of cold resistance of plants, the gene belongs to a tea tree NAC transcription factor family, and the nucleotide sequence of the gene is shown as SEQ ID NO.1. The tea tree CsNAC25 gene negatively regulates the cold resistance of the tea tree, and the gene expression is reduced under low temperature induction. The over-expression of the gene in tobacco weakens the cold resistance of a transgenic plant, and the expression of CsNAC25 is silenced by adopting a VIGS technology in a tea tree, so that the cold resistance of the tea tree is remarkably improved. Silencing CsNAC25 effectively reduces the wilting degree and the ion leakage rate of tea seedling leaves under low temperature stress, activates a key CBF signal channel and promotes accumulation of osmoregulation substances. The application of the application is expected to effectively improve the cold resistance when low-temperature disasters such as late spring coldness occur, and has important theoretical significance and wide application prospects for guaranteeing the stable yield and quality of the tea industry.

Disclosed herein are compositions comprising LNPs capable of selectively targeting cancer cells in organs for the efficient delivery of RNA cargos, specifically aimed at targeting poorly druggable, disease-driving transcription factors in prostate cancer and methods of use thereof.

Disclosed herein are methods of gene editing, or endogenous suppression, of cytokines / chemokines / transcription factors secreted from chimeric antigen receptor (CAR)-bearing immune effector cell such as CAR-T cells for the mitigation of cytokine release syndrome and / or CAR-T associated neuropathy. These methods involve insertion of the CAR into a locus of a cytokine gene, blocking its expression. Also disclosed herein are (CAR)-bearing immune effector cells with CARs inserted into a locus of a cytokine gene, and methods of treatment of diseases with immunotherapy with a reduced incidence of cytokine release syndrome and / or CAR-T associated neuropathy.

The invention provides an application of a ganoderma lucidum transcription factor yabby1 in regulating and controlling the transcriptional activity of a ganoderic acid synthetic gene SQS and a method, and belongs to the field of gene engineering. The nucleotide sequence of the yabby1 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the yabby1 gene is as shown in SEQ ID NO. 2; the ganoderma lucidum transcription factor yabby1 gene and the ganoderma lucidum SQS gene are constructed on a recombinant vector and are introduced into cells, so that the expression of the ganoderma lucidum SQS gene can be activated, and the content of ganoderic acid in ganoderma lucidum can be increased.

The invention provides a gene regulation network inference method based on second-order multi-channel directed graph convolution, and belongs to the technical fields of graph representation learning, system biology and the like. Firstly, a first-order proximity matrix, a second-order in-degree proximity matrix and a second-order out-degree proximity matrix are constructed respectively, an existing method is expanded through the second-order proximity matrix, secondly, feature information implied in the three matrixes and expression data is extracted by using multiple graph convolution networks respectively, and the feature information of the three matrixes and the expression data is extracted. And then fusion operation is carried out to fuse features extracted by the multi-channel directed graph convolutional network. And finally, respectively extracting embedding expressions of transcription factors and target genes in potential regulatory gene pairs by using two channels, and obtaining a predicted regulatory probability through dot product operation. According to the method, the prior regulation and control information and the directional regulation and control information implied in the expression data are extracted, and the perception domain of the graph convolution is expanded from the first order to the second order, so that richer feature information can be extracted, and the inference accuracy of the potential directional regulation and control relationship is improved.

The invention discloses an application and a method of an overexpression transcription factor PtoLBD1.2 gene in promoting growth and development of populus tomentosa, a nucleotide sequence of the transcription factor PtoLBD1.2 gene is shown as SEQ ID NO.3. A transgenic plant is overexpressed through transgenic tissue specificity PtoLBD1.2, a plant with high expression quantity is screened out through subsequent positive identification for phenotypic observation, and a result shows that the transcription factor PtoLBD1.2 gene can be used as an overexpression transcription factor PtoLBD1.2 gene for promoting growth and development of populus tomentosa. Compared with the wild type in the same period, the transgenic plant of PtoLBD1.2 has the advantages that the plant height is increased, the stem is thickened, and the aboveground biomass is obviously improved. According to the cultivation method provided by the invention, the high-quality poplar variety with high growth speed and high biomass is cultivated. The method has great significance in molecular breeding and cultivation of excellent poplar in the future.

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The invention discloses application of Bombyx mori neuropeptide BommoOKAtype4 in preparation of a medicine for inhibiting melanoma cell growth, and belongs to the technical field of medical biology. Aiming at the technical problems that melanoma is high in malignancy degree, easy to transfer and limited in treatment means, and whether the bombyx mori neuropeptide BommoOKAtype4 can directly inhibit the growth of melanoma cells is not clear yet when the bombyx mori neuropeptide BommoOKAtype4 is mainly used for skin whitening before, the invention provides the application of the bombyx mori neuropeptide in preparing the medicine for inhibiting the melanoma cells. According to the silkworm neuropeptide, gene expression and protein functions of melanin synthesis key enzymes TYR, TRP1 and TRP2 are comprehensively inhibited by down-regulating expression of a core transcription factor MITF, so that melanoma cell proliferation is directly inhibited while melanin synthesis is inhibited, and therefore, the silkworm neuropeptide can be used for preparing drugs for treating melanoma and has a wide application prospect. A novel peptide candidate substance is provided for treatment of melanoma.

Peptides and methods of use thereof, are disclosed for use in treating various diseases and disorders, including inflammation, pain, oral mucositis, oral lesions, and cancer. The peptides modulate the activity of the transcription factor NF κB.

To provide novel methods and uses for increasing a yield of recombinant proteins in host cells, which are simple, efficient and suitable for use in industrial methods.SOLUTION: Provided is a method of increasing a yield of a protein of interest (POI) in an eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4 / 2. Further provided are a recombinant eukaryotic host cell for manufacturing the POI, the host cell being engineered to overexpress at least one polynucleotide encoding at least one transcription factor, as well as the use of the host cell for manufacturing the POI.SELECTED DRAWING: None

The invention discloses liver-responsive synthetic macrophages and a construction method and tumor immunotherapy application thereof, and belongs to the technical field of biological medicines. The system is an IBMDM cell line of a stable expression alpha SLC17A2-P65 / shSIRP alpha-synM system, and comprises an extracellular domain and an intracellular domain, the extracellular domain is composed of a single-chain antibody scFv for recognizing an antigen SLC17A2 and a transcription factor GAL4-VP16 (GV), and the intracellular domain is composed of a UAS promoter sequence recognized by the GV and P65 / shSIRP alpha. When the antibody responds to the liver, GV is released through the self-cleavage effect of synNotch, and the GV recognizes a UAS promoter sequence after entering a nucleus to activate downstream gene expression. The invention also provides a construction method of the system. The construction method comprises the following three steps: constructing a vector plasmid, packaging lentivirus and constructing a target cell line. The system can be applied to screening anti-liver cancer immunotherapy drugs, and a new research tool is provided for liver cancer treatment.

The present invention relates to inhibitors of NFkappaB activity for the treatment of diseases and disorders, and discloses peptides and methods of use thereof for the treatment of various diseases and disorders, including inflammation, pain, oral mucositis, oral lesions, and cancer. The peptides modulate the activity of the transcription factor NFkappaB.

Methods and systems are provided for determining sarcomatoid differentiation of renal cell carcinoma (RCC). In various embodiments, the present disclosure relates to the use of one or more histone modifications, DNA methylation, and / or expression of one or more transcription factors that are characteristic of RCC with sarcomatoid features (sRCC). In some embodiments, differential modifications are detected and quantified at one or more genomic loci. In various embodiments, a determination of sarcomatoid subtype is useful, e.g., for identifying, selecting treatment for, and / or treating a subject having sRCC.

The invention discloses an application of a rice B3 transcription factor family gene LOCOs01g67830 and an encoding protein thereof in regulation and control of pre-harvest germination of rice. The invention further discloses a method for regulating and controlling pre-harvest germination of rice. The invention finds that the editing of the gene affects the pre-harvest germination condition of rice by evaluating the agronomic traits of the rice for editing LOCOs01g67830 created by a CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / Cas9) technology. The gene and the operation technology provided by the invention have an obvious effect in the aspect of regulating and controlling rice pre-harvest sprouting, and have extremely high application value. Experiments prove that the mutation of the LOCOs01g67830 can cause increase of the pre-harvest germination phenomenon, so that the gene LOCOs01g67830 is a rice pre-harvest germination regulation gene, a new candidate gene is provided for research on rice pre-harvest germination, and the gene has very important application value in the aspects of improving the rice yield and promoting agricultural production.

The present invention belongs to the field of genetic engineering technology and discloses a transcription factor RsRAP2-12 for regulating radish anthocyanin synthesis and its application. The nucleotide sequence of the RsRAP2-12 gene is such as SEQ ID No. 1, which can be used to regulate the synthesis of radish anthocyanins. The present invention also obtains an overexpression vector and a VIGS silencing vector for the RsRAP2-12 gene; and proves that transient overexpression of RsRAP2-12 on radish leaves can increase the anthocyanin content on the leaves, and injecting the constructed VIGS silencing vector into the roots of red-skinned, red-fleshed radishes can reduce the accumulation of anthocyanins. Therefore, the RsRAP2-12 gene plays an important role in regulating the synthesis of radish anthocyanins. The present invention is of great significance for breeding new radish varieties with high anthocyanin content and provides strong technical support for improving radish quality.