125 results about "Transcription factor" patented technology

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention provides application of a tobacco NtMYC1 gene in promoting growth and development of tobacco glandular hair, and belongs to the technical field of plant breeding. The tobacco MYC1 transcription factor is obtained by carrying out sequence characteristic analysis, conservative structural domain identification, protein physicochemical property analysis and phylogenetic analysis on a genome of tobacco. And an RNAi technology and an overexpression tobacco transgenic line verify that the tobacco MYC1 transcription factor and the coding gene thereof have the effect of regulating and controlling the growth and development of the tobacco glandular hair, and the growth and development of the tobacco glandular hair are promoted through forward regulation and control, so that the growth and development of the tobacco glandular hair are inhibited through silent expression. Therefore, the tobacco MYC1 transcription factor and the coding gene thereof provided by the invention provide a new means for tobacco breeding.

The invention provides application of a transcription factor LcNF-YB3 of lycoris chinensis in regulating and controlling the flowering time of plants, and belongs to the technical field of molecular breeding. The invention provides a lycoris chinensis transcription factor LcNF-YB3. The amino acid sequence of the lycoris chinensis transcription factor LcNF-YB3 is as shown in SEQ ID NO: 1. The Lycoris chinensis transcription factor LcNF-YB3 delays plant flowering through forward regulation, the flowering time of an arabidopsis thaliana plant for heterologous expression of LcNF-YB3 is delayed, rosette leaves are increased, and the flowering time of the plant Lycoris chinensis is obviously advanced by silencing the LcNF-YB3 gene in Lycoris chinensis. The Lycoris chinensis transcription factor LcNF-YB3 can effectively regulate and control the flowering time, solves the problems of asynchronous flowering phases and the like in breeding, and provides a new genetic resource for the improvement of Lycoris and other bulb flower varieties.

The invention discloses application of Bombyx mori neuropeptide BommoOKAtype4 in preparation of a medicine for inhibiting melanoma cell growth, and belongs to the technical field of medical biology. Aiming at the technical problems that melanoma is high in malignancy degree, easy to transfer and limited in treatment means, and whether the bombyx mori neuropeptide BommoOKAtype4 can directly inhibit the growth of melanoma cells is not clear yet when the bombyx mori neuropeptide BommoOKAtype4 is mainly used for skin whitening before, the invention provides the application of the bombyx mori neuropeptide in preparing the medicine for inhibiting the melanoma cells. According to the silkworm neuropeptide, gene expression and protein functions of melanin synthesis key enzymes TYR, TRP1 and TRP2 are comprehensively inhibited by down-regulating expression of a core transcription factor MITF, so that melanoma cell proliferation is directly inhibited while melanin synthesis is inhibited, and therefore, the silkworm neuropeptide can be used for preparing drugs for treating melanoma and has a wide application prospect. A novel peptide candidate substance is provided for treatment of melanoma.

To provide novel methods and uses for increasing a yield of recombinant proteins in host cells, which are simple, efficient and suitable for use in industrial methods.SOLUTION: Provided is a method of increasing a yield of a protein of interest (POI) in an eukaryotic host cell, preferably a yeast, by overexpressing at least one polynucleotide encoding at least one transcription factor of the present invention, preferably Msn4 / 2. Further provided are a recombinant eukaryotic host cell for manufacturing the POI, the host cell being engineered to overexpress at least one polynucleotide encoding at least one transcription factor, as well as the use of the host cell for manufacturing the POI.SELECTED DRAWING: None

The present invention relates to inhibitors of NFkappaB activity for the treatment of diseases and disorders, and discloses peptides and methods of use thereof for the treatment of various diseases and disorders, including inflammation, pain, oral mucositis, oral lesions, and cancer. The peptides modulate the activity of the transcription factor NFkappaB.

The invention belongs to the technical field of agricultural biology, and provides a soybean nodulation and nitrogen fixation related gene and application of an encoding protein thereof, the nucleotide sequence of the gene Gm02g072800 is shown as SEQ ID NO: 1, or a variant sequence which encodes the same functional protein and has homology greater than or equal to 90%; the amino acid sequence of the protein coded by the gene Gm02g072800 comprises a conservative AP2 structural domain, belongs to an ERF transcription factor family, is positioned in a cell nucleus, and has the functions of promoting soybean root nodule development and symbiotic nitrogen fixation. According to the invention, the specific expression of the gene in the soybean middle column and root nodule endodermis is determined, and a target gene is provided for cultivating soybean varieties with high nitrogen fixation efficiency.

The present application relates to a kind of rice transcription factor OsLSD5 and its application in anti-rice stripe virus, by the overexpression OsLSD5 vector after being transferred in plant rice, obtain the stable genetic OsLSD5-ox transgenic plant.

The invention provides a nucleic acid construct which comprises an immune response regulatory sequence and a coding sequence of a reporter gene driven by a promoter sequence, and the immune response regulatory sequence comprises a transcription factor binding regulatory element RE which is co-stimulated and regulated by transcription factors AP-1 and CD28. The invention also provides an in-vitro immune effector function reporter gene cell model containing the nucleic acid construct, and the cell model can realize effector function activity determination performance of sensitive and potent signals so as to evaluate ADCC and ADCP killing action mechanisms and titers mediated by antibody-dependent disease cells designed by an Fc structural domain of a therapeutic antibody product. In addition, the immunosuppression and regulation efficacy of a targeted CTLA-4 or Abatacept mediated treatment method on a CD28 co-activation pathway can be evaluated.

The invention relates to the application of inhibition of transcription factor 4 (TCF4 / ITF2) in the treatment of cancer. This in particular in combination therapy in conjunction with an immunotherapeutic compound (such as for treating cancers poorly responding or refractive to immunotherapy). In particular, inhibition of TCF4 / ITF2 is capable of restoring response to immunotherapy such as immune checkpoint inhibitor therapy. Expression levels of TCF4 / ITF2 as well as levels of mesenchymal-like cancer cells in a cancer lesion are predictive of future response to immunotherapy early after initiation of the immunotherapy. Further part of the invention are methods of detecting mesenchymal-like cancer cells.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

T lymphocytes play a key role in the immune response and their functions are intimately linked to metabolic programs. During immune responses, T cells undergo a metabolic reprogramming notably characterized by an increased aerobic glycolysis. Using a quantitative phosphoproteomic approach, the inventors have identified a new transcription factor called Foxk1 as being highly phosphorylated in T cells upon T Cell Receptor (TCR) engagement. The results also indicate that Foxk1 phosphorylation and nuclear translocation is dependent of the AKT-mTOR kinase activities. Using T-cell specific Foxk1 deficient mice (Foxk1- / -), we demonstrated that Foxk1 is required for full T cell activation. Foxk1-deficient T cells exhibited reduced proliferation and cytokine secretion following TCR stimulation. Furthermore, T cells from Foxk1- / - mice were less prone to acquire an effector like phenotype than wild-type cells when challenged in vivo. Conversely, Foxk1 overexpression in T cells enhanced their effector functions in a TCR-dependent manner. In CD8+ T cells, this effect also results into enhanced cancer cell killing capacity in vitro, and improved tumor rejection in vivo. Altogether, these results indicated that Foxk1 is a major regulator of T cell metabolism and thus, of T cell effector functions. Thus, the present invention relates to host immune cells engineered to overexpress a Foxk1 polypeptide and their use of the treatment of cancer.

The MYC and NMYC transcription factors (TFs) play a key role in cell proliferation and are overexpressed in most cancer cells. However, in normal cells their overexpression triggers safeguard mechanisms promoting cell death and cellular senescence, which are bypassed in cancer cells. Here, the inventors reveal that in normal cells MYC binds to the Inositol 1,4,5-Trisphosphate Receptor type 1 (ITPR1) gene and upregulates its expression, triggering an ER-mitochondria calcium (Ca2+) transfer, which is involved in MYC-induced cell death and senescence. Supporting a tumor suppressive role of MYC / ITPR1 axis, ITPR1 expression is generally decreased in cancer and reactivation of this pathway induces cancer cell death. Nevertheless, some cancer cells, generally expressing high levels of MYCN and / or MYC, also express high level of ITPR1, which correlates with high expression of BCL2, encoding an inhibitor of ITPR1. Strikingly, in high-risk MYCN-amplified neuroblastoma, ITPR1 expression is controlled by NMYC and its level correlates with worse patient survival. In these cells, blocking the interaction between BCL2 and ITPR1, via an BCL2-BH4 domain inhibitor induces mitochondrial Ca2+ accumulation and cell death, and decreases tumor size. Thus, the present invention relates to a method for treating MYChigh cancer, and in particular NMYC--amplified neuroblastoma in a subject by administering an BCL2-BH4 domain inhibitor.

This invention provides a novel screening method for signaling pathway activators and inhibitors based on cell viability / death phenotypes. Specifically, this invention provides genetically engineered cells that enable large-scale screening of signaling pathway activators and inhibitors based on the expression of protective and suicide genes driven by signaling pathway transcription factors. The viability / death phenotype reporter system is effective and convenient for screening drugs affecting signaling pathways and can be applied to high-throughput screening in the early stages of drug development.

The present invention features promoters providing for DNA transcription. The promoters comprise: (1) a promoter core derived from the human peptidyl-prolyl isomerase G (PPIG) promoter, ATP citrate lyase (ACLY) promoter, actin gamma (ACTG1) promoter, actin related protein 2 (ACTR2) promoter, actin related protein 2 / 3 complex subunit 2 (ARPC2) promoter, or the vinculin (VCL) promoter; and / or (2) ocular specific transcription factor binding motifs from CRX, OTX2, and / or NRL.

The invention discloses a soybean GmHRF-1 gene and application of protein coded by the soybean GmHRF-1 gene in improvement of soybean regeneration frequency and genetic transformation efficiency, and relates to the technical field of plant genetic engineering. The invention identifies and verifies that the GmHRF-1 gene in the soybean B3 transcription factor family has a brand new function of remarkably improving the regeneration capacity of somatic cells for the first time. By introducing the gene into soybean recipient cells and overexpressing the GmHRF-1, the regeneration frequency and genetic transformation efficiency of soybeans can be remarkably improved. The invention provides an efficient and universal endogenous gene for breaking through the technical bottleneck of genetic transformation of backbone germplasm which is difficult to regenerate of soybeans. The research result not only provides a new gene target for analyzing a molecular mechanism of soybean stem tip meristem regulation and regeneration, but also provides a technical support for constructing an efficient and stable soybean genetic transformation system.

The application belongs to the technical field of biological medicine, and particularly relates to application of a KAT5 inhibitor in a medicine for enhancing the immunotherapy effect of natural killer cells, and a medicine for enhancing the immunotherapy effect of natural killer cells. The KAT5 inhibitor comprises at least one of Nu9056, TH1834 and MG149. The KAT5 inhibitor can inhibit the recruitment of a transcription factor SP1 to an ADAM10 promoter, reduce the transcriptional activity of ADAM10, reduce the expression of NKG2D ligands on the cell surface, and thus enhance the sensitivity of tumor cells to NK cell killing. The application first discloses a new mechanism in which KAT5 regulates an ADAM10-NKG2D signal axis through an epigenetic mechanism, and proves that the KAT5 inhibitor can significantly enhance the immunotherapy effect of NK cells in vitro and in vivo. The application provides a brand-new target and strategy for developing a new immunosensitizer and a combined cell therapy.

Specific proteins catalyze specific reactions, transport specific molecules, and form specific cellular structures and changing their presence and relative abundance can be applied, for example to improve human health, animal health, crop health, carbon capture, agricultural productivity and protection, and biologic activity. However, precisely effectuating phenotypic changes and outcomes is expensive and error prone; and precisely changing protein expression in an organism has been challenging due to limited ability to control experimental factors, limitations of measurement, the exponential number of ways to affect gene expression of a protein, and limited precision and accuracy in predicting changes to expression when modifying transcription factors, promoters, and GC content. This disclosure introduces methods of gene training to help enable more direct, expedient and successful product development pipelines to digitally adjust or aim expression level of one or more proteins, such as for improving crops, carbon capture, biologics, research and development, and human health.

The invention relates to the technical field of plant genetic engineering, in particular to application of a plant HSF family transcription factor HSFA2B in regulation and control of temperature response growth of plant mesocotyl. The invention provides application of a plant HSF family transcription factor HSFA2B in regulating and controlling temperature response growth of mesocotyl in a plant seedling stage or regulating and controlling direct seeding emergence of plants at high environment temperature. The invention finds that HSFA2B has an important function in rice mesocotyl response temperature growth regulation and control. The mesocotyl of the mutant with the HSFA2B knocked out is obviously longer than that of the wild type at the proper environment temperature, but the mesocotyl of the mutant with the HSFA2B knocked out is not obviously different from that of the wild type at the high environment temperature, and the response sensitivity to the high environment temperature is reduced. The discovery of the new function of the HSFA2B is of great significance to the breeding and direct seeding production of rice direct seeding varieties.

The invention relates to a rice transcription factor OsMTA1 and application of the rice transcription factor OsMTA1 in resisting rice viruses, and after an overexpression OsMTA1 vector is transferred into plant rice, a transgenic plant with stable inheritance is obtained. The result shows that compared with the control Nipponbare rice, the transgenic plant shows higher disease resistance.

The application belongs to the field of biological genetic engineering and relates to application of PgMYC3 in regulation of ginsenoside biosynthesis. The sequence of the ginseng transcription factor PgMYC3 is shown as SEQ ID NO. 2. The application clones the PgMYC3 gene, constructs a plant expression vector, and adopts the Agrobacterium-mediated transformation method to transform ginseng callus. Through RT-qPCR technology identification, positive callus overexpressing PgMYC3 is obtained. In the transgenic ginseng callus, the total saponin content is increased by 1.5 times, which indicates that PgMYC3 has a promoting effect on the accumulation of ginsenosides. Meanwhile, through molecular biology technology, it is confirmed that PgMYC3 can directly bind to the G-box element to activate the expression of the saponin synthesis gene PgPPDS.

The invention belongs to the technical field of biological medicine, provides a mustard gas immunotoxicity evaluation method based on Foxp3 promoter methylation, and evaluates the application effect of the Foxp3 promoter methylation level in mustard gas immunotoxicity evaluation in combination with in-vitro cell experiments and in-vivo animal experiments. Results show that Treg cell deficiency is a main reason for tissue damage and inflammation caused by mustard gas poisoning, cell fate and functions of the Treg cell deficiency are mainly regulated and controlled by Foxp3 transcription factors, mustard gas can cause increase of the DNA methylation level of Foxp3 gene loci, DNA hypermethylation of Foxp3 promoters is crucial to Treg cell differentiation inhibition, Th17 / Treg imbalance and systemic inflammation caused by mustard gas exposure, and the Treg cell deficiency is a main reason for tissue damage and inflammation caused by mustard gas poisoning. The methylation level of the Foxp3 promoter can be used as a marker of a mustard gas immunotoxicity evaluation method.

This invention belongs to the field of plant molecular biology, specifically relating to a small RNA molecule that alleviates lignification of loquat fruit and its application. The small RNA molecule is from loquat. Eja-MIR164 Its nucleotide sequence is shown in SEQ ID NO. 6. After processing within the plant, it forms a functional mature form. This invention experimentally confirms that loquat fruit contains… Eja-MIR164 The expression level of Eja-miR164 was negatively correlated with lignin content; transient interference or stable transformation using STTM technology reduced Eja-miR164 content, targeting its genes NAC transcription factor and lignin polymerase gene. EjLAC7 Increased expression levels in all samples led to a significant increase in lignin content. This invention utilizes small RNA molecules. Eja- MIR164 It can be used to breed new loquat varieties with reduced lignification, improve fruit texture and quality, and enhance post-harvest storage performance, and has potential application value in loquat genetic improvement and molecular breeding.

A population of universal natural killer (NK) cells derived from human pluripotent stem cells (hPSCs) and engineered to overexpress the transcription factor ID2, NFIL3, and / or SPI1 and, optionally, an anti-programmed death ligand 1 (PD-L1) chimeric antigen receptor (CAR) and an anti-fluorescein isothiocyanate CAR are provided. Methods of treating cancer in a subject using the population of universal NK cells are also provided.

The application discloses a rice transcription factor OsRAV1 application in regulating seed storage tolerance and salt tolerance in germination stage, belongs to the field of plant molecular biology and plant breeding. OsRAV1 The application discloses that the gene regulates seed vigor, and knockout OsRAV1 will significantly reduce seed vigor, and the seed is not storage tolerant. OsRAV1 significantly improves seed vigor after storage and live seedling rate under salt stress. The application can be used to solve the problems of rice seed not storage tolerant, low seedling rate, uneven seedling and poor abiotic stress tolerance in the live process, and has wide application prospect.

The invention discloses a biomarker combination based on pollutant exposure detection and application thereof, and relates to the technical field of biomarkers, the biomarker combination comprises an integrin subunit itg-alpha11, a nuclear transcription factor nf-kb, and an antioxidant stress factor nrf2; according to the biomarker combination disclosed by the invention, a complete association network from molecular response to phenotypic risk is constructed by accurately capturing an action mechanism of BDE-47 at molecular and cellular levels; the rapid diagnosis of pollutant exposure can be realized in the early stage of embryonic development, and the risk of development malformation and the like can be effectively early warned; and meanwhile, a quantitative basis is provided for evaluating the pollution remediation effect, and accurate formulation of an environment quality standard and optimization of an aquaculture management strategy are supported. The biomarker combination not only deepens the theoretical cognition of a pollutant toxicity mechanism, but also provides an innovative tool for environmental health risk assessment and ecological protection practice, and has important theoretical significance and wide application prospects.

The application discloses a breeding method for synthesizing resveratrol and pterostilbene in rice seed endosperm, and relates to the technical field of biology.The transgenic breeding method comprises the steps of transforming a recombinant carrier into rice callus and then cultivating the rice callus into a transgenic rice plant; the recombinant carrier comprises a gene expression cassette for expressing VvSTS protein, a gene expression cassette for expressing VvROMT protein, a gene expression cassette for expressing At4CL protein and a gene expression cassette for expressing transcription factor OsMYBP1 protein.The application realizes the synthesis of resveratrol and pterostilbene in rice endosperm, and the product can be used as raw material for producing resveratrol and pterostilbene, and has important guiding significance for new functional breeding.

This invention discloses the application of MITF inhibitors in the preparation of drugs for the prevention and / or treatment of sorafenib cardiotoxicity, belonging to the field of biomedical technology. This invention is the first to discover that the transcription factor MITF is a core regulatory factor of sorafenib cardiotoxicity. Sorafenib can specifically upregulate the expression and transcriptional activity of MITF in cardiomyocytes, and its expression level is positively correlated with the degree of cardiomyocyte damage. By inhibiting the expression or activity of MITF, sorafenib-induced cardiomyocyte hypertrophy and damage can be significantly reversed. This invention provides a novel specific intervention target for the prevention and treatment of sorafenib cardiotoxicity, and has significant scientific and clinical translational value.

The invention discloses an application of an OsmiR159-GAMYBL2-RGA1 signal channel in regulation and control of drought tolerance of rice, and particularly relates to an application of OsmiR159 in directly targeting and inhibiting expression of an OsGAMYBL2 transcription factor, and an OsGAMYBL2 protein is combined with a promoter of a G protein alpha subunit coding gene RGA1 and inhibits transcription of the promoter, so that a complete OsmiR159-OsGAMYBL2-RGA1 signal transduction chain is formed. Based on the application, an efficient molecular target and an enforceable technical scheme are provided for drought-resistant breeding of rice.