770 results about "Signalling molecules" patented technology

The present invention relates to a class of molecules specified as novel multipurpose antibody derivatives. This class of molecules is created by heterodimerization of two constituting components. Heterodimerization is obtained by the specific heterotypic interaction of a chosen VH-CH1 combination of immunoglobulin domains, with a chosen VL-CL combination of immunoglobulin domains. The appropriate VH and VL domains in the VHCH1 and VLCL context, a binding specificity can be constitituted by the heterodimerization scaffold itself. One or both of the comprising VHCH1 and VLCL chains can thus be extended at either the N- or the C-terminus or both with other molecules, such as a toxin polypeptide, an enzyme, a hormone, a cytokine, a signaling molecule, or a single chain linked Fv fragment with the same or a different specificity.

This invention is directed to methods and compositions for diagnosis, prognosis and for determining methods of treatment. The physiological status of cells present in a sample (e.g. clinical sample) can be used in diagnosis or prognosis of a condition (e.g. Chronic Lymphocytic Leukemia), in patient selection for therapy, to monitor treatment and to modify or optimize therapeutic regimens. The physiological status of a cell can be determined by comparing the intracellular status of one or more activation elements (e.g. the phosphorylation status of a signaling molecule) in a cell (e.g. a cancer cell) to that of another cell (e.g. a normal cell). The physiological status of a cell can be further classified by adding one or more modulators (e.g. an inhibitor or activator) to the cell in question. In some embodiments, the invention is directed to methods of determining a phenotypic profile of a population of cells.

The invention discloses a SERS biological probe and a preparation method thereof. The SERS biological probe provided by the invention comprises a SiO2 core and a metal nanoparticle layer on the surface of the SiO2 core, wherein a plurality of Raman signal molecules connected with the metal nanoparticle layer; a SiO2 layer is also provided outside the metal nanoparticle layer; the Raman signal molecules are positioned between the metal nanoparticle layer and the SiO2 layer; the SiO2 layer surface is modified with biological probe molecules; and the metal nanoparticle layer is Au nanoparticle layer or Ag nanoparticle layer. The SERS biological probe provided by the invention has wide generality, and has wide application prospect in identification and detection of biological molecules (DNA molecules and protein molecules), rapid disease diagnosis, biomedical imaging technology as well as in the fields of serious disease treatment, food hygiene, environmental monitoring, etc.

A population of labeled probes is provided that utilize an encoding system in which both the intensity and specific characteristics of a signal molecule are utilized to reduce the number of signal molecules necessary to identify each member of the population of probes. In the population of labeled probes, each labeled probe includes a probe associated with a series of detectably distinguishable signal molecules. The number and type of signal molecules identifies the associated probe, and the number of probes in the population exceeds the number of unique signal molecules. The population of probes are used in methods of the invention and reaction mixtures of the invention, for identifying a target molecule and for sequencing a nucleic acid molecule, for example.

This invention relates to specific antibodies against ganglioside GD3 called MB3.6 and against protein prostate specific membrane antigen (PSMA) called 3D8, 4D4 and 3E11 when prepared as chimeric molecules with signaling molecules of T cells and other effector cells, and the use thereof in the treatment of cancers expressing these antigens.

The invention relates to a surface-enhanced Raman scattering probe and a preparation method thereof. The probe is an interlayer structure and consists of a core precious-metal nano-rod, an intermediate sandwich layer and a precious-metal shell layer which grows and is formed on the outer surface, wherein a Raman signal molecule is wrapped inside the intermediate sandwich layer. The preparation method comprises the following steps that: the surface of the precious-metal nano-rod absorbs or is coupled with the Raman signal molecule, then the Raman signal molecule is wrapped to form the intermediate sandwich layer through silicon dioxide or polyelectrolyte, and then the precious-metal shell layer grows and is formed outside the intermediate sandwich layer. Through the surface-enhanced Raman scattering probe with the sandwich structure, strong local electromagnetic density is produced through the interaction of the surface plasma between the gold nano-rod and the outer metal shell layer, so the Raman signal is greatly enhanced, the weaknesses that a two-dimensional underlay is difficult to repeat, is expensive and is complicated can be overcome, and at the same time the size of the surface-enhanced Raman scattering probe is smaller than 200nm, biological detection of living bodies and application of biological imaging can be facilitated when the surface-enhanced Raman scattering probe is used as a biological probe.

The invention relates to the modulation of gene expression in a cell, also called gene control, in particular in relation to the treatment of anthrax. The invention provides a method for modulating expression of a gene in a cell comprising providing the cell with a signaling molecule comprising a peptide or functional analogue thereof.

The invention relates to the modulation of gene expression in a cell, also called gene control, in particular in relation to the treatment of a variety of diseases. The invention provides a method for modulating expression of a gene in a cell comprising providing the cell with a signaling molecule comprising a peptide or functional analogue thereof. Furthermore, the invention provides a method for identifying or obtaining a signaling molecule comprising a peptide or functional derivative or analogue thereof capable of modulating expression of a gene in a cell comprising providing the cell with a peptide or derivative or analogue thereof and determining the activity and / or nuclear translocation of a gene transcription factor.

The present invention comprises compositions, methods, and devices for delivering angiogenic factors and signaling molecules to a target tissue, and controlling the release of these factors and signaling molecules to spatially and temporally restrict their release and dissemination, for the purposed promoting angiogenesis in target tissues.

A recombinant phage system has been developed for the rapid detection of bacteria, particularly fecal coliform indicator bacteria. The systems of the invention link phage infection events to quorum sensing signal molecule biosynthesis and bioluminescent bioreporter induction, facilitating the detection of pathogens that may be present in low numbers. The phage-based systems of the invention maintain specificity for the pathogen while still producing significant signal amplification for sensitive and quantitative detection. The systems require only the combination of sample with phage and bioreporter organisms; no extraneous addition of any substrates or user intervention of any kind is necessary, making this approach significantly less technical than standard molecular or immunological methods.

The invention discloses a method for preparation of an addressable electrochemical transducer array, and determination multiple tumor markers in a sample by adopting the addressable electrochemical transducer array. The method comprises the following steps: printing a row electrode and a line electrode on a proper substrate material by preparing proper ink; selecting a proper paper material, preparing a reaction hydrophobic region and a hydrophilic region on the paper by utilizing a wax printing technology, and constructing a reference electrode and a counter electrode shared by an array electrode on the other paper-based material through a screen printing technology; functionalizing the prepared hydrophilic region, identifying an antigen, and marking a signal molecule; assembling the prepared working electrode, the reference electrode and the counter electrode with the prepared reaction region, and constructing the addressable electrochemical transducer array; and dripping an enzyme substrate in the reaction region, and adding phosphoric acid buffer liquid on the paper layer printed with the reference electrode and the counter electrode, and detecting the target.

A novel nucleic acid construct for down-regulating angiogenesis in a tissue of a subject is provided. The nucleic acid construct includes: (a) a first polynucleotide region encoding a chimeric polypeptide including a ligand binding domain fused to an effector domain of an apoptosis signaling molecule; and (b) a second polynucleotide region encoding a cis acting regulatory element being for directing expression of the chimeric polypeptide in a specific tissue or cell; wherein the ligand binding domain is selected such that it is capable of binding a ligand present in the specific tissue or cell, whereas binding of the ligand to the ligand binding domain activates the effector domain of the apoptosis signaling molecule. Also provided are methods of utilizing this nucleic acid construct for treating diseases characterized by excessive or aberrant neo-vascularization or cell growth.

The invention discloses a method for preparing an electrochemical luminescence paper chip and a method for measuring content of signal molecule hydrogen sulfide in MCF-7 by using an electrochemical luminescence sensor. The method is characterized by preparing a hydrophobic region, a semi-hydrophobic region, a hydrophilic region and a hollow channel on paper by utilizing a wax printing technology, cutting the hollow channel by virtue of a laser cutting machine, preparing proper printing ink, printing a corresponding reference electrode and a working electrode on the paper, functionalizing the working area, dripping stock solution in a pretreatment region, and fixing cancer cells; and folding the prepared paper chip, forming a three-electrode system, dropwise adding electrolyte solution in a reaction region, connecting an electrochemical workstation, thus realizing high-sensitivity detection of the low-content signal molecules in the cancer cells.

The invention discloses a construction method of an anticoagulant artificial blood vessel scaffold material. The method includes: taking a rotating stainless steel pipe as a receiver, employing an electrospinning technology to prepare an organic macromolecular polymer, a mixture of a Cu2<+> complex catalyst and the organic macromolecular polymer, and the organic macromolecular polymer successively into a three-layer structured superfine fiber porous tubular scaffold material; and regulating the types and proportion of the Cu2<+> complex catalyst and the organic macromolecular polymer, as well as the electrospinning time of a three-layer electrospinning solution so as to control the release rate of NO. With a three-layer structure, the blood vessel scaffold material formed by the invention not only realizes loading of the Cu2<+> organic complex catalyst, but also improves the phenomenon of NO burst release catalyzed by an ordinary hybrid electrospun structure scaffold material, improves the loading stability of the Cu2<+> complex catalyst, and can leave the biological signal molecule NO to play a better role in inhibiting platelet adhesion, resisting platelet activation and inhibiting smooth muscle cell proliferation so as to improve its anticoagulant property.

Compositions and methods are provided for ameliorating various effects of UVA and UVB radiation, especially from the sun. The compositions include an ingredient that prevents photoaging from MED and subMED radiation, namely an MMP (matrix metalloproteinase) inhibitor, especially formulated for oral administration, and more especially formulated for controlled-release so as to provide the MMP inhibitor when MMP induction (including upstream signalling molecules like c-JUN, and / or MMPs like stromelysin) is most prevalent.

An artificial antigen presenting cell includes a liposome having at least one recombinant soluble MHC-peptide complex incorporated therein. The artificial antigen presenting cell may also include at least one additional signal molecule incorporated therein for manipulating the intensity and quality of the immune response. The recombinant soluble MHC molecule is obtained by a method utilizing PCR amplification of gDNA or cDNA, and a tag is attached thereto for anchoring the recombinant soluble MHC molecule to the liposome.

The invention discloses a mercury ion detection reagent and a detection method. The reagent comprises nano sol, nucleic acid adaptors marked by Raman active molecules and a charge modifier. The detection method comprises the following steps of: firstly, synthesizing the nano sol, then adding a proper quantity of adaptors to a proper quantity of fresh nano sol and uniformly mixing; continuing to add a proper quantity of charge modifier and uniformly mixing; then adding a sample to be tested and incubating for 1-5 minutes; and finally, taking the nano sol to test a Roman scattering spectral signal and carrying out data processing. In the invention, based on the addition of Hg<2+>, the structures of the nano particle surface nucleic acid adaptors are changed, i.e. nano particle aggregation in the silver sol and surface enhanced Roman scattering signals of signal molecules are enhanced. In the invention, the SERS (Surface Enhanced Raman Scattering) technology is adopted, the detection limit to the Hg<2+> is at the nmol / L level, thus the invention has the advantages of high detection speed of the linear response range of Hg<2+> concentration and high selectivity and is suitable for the quick detection of trace Hg<2+> in environmental water.

The invention belongs to the technical field of sewage treatment and relates to a method for enhancing aerobiotic ammonia oxidizing bacterium (AOB) gathering through N-acylated homoserine lactone (AHLs) compounds. The method includes the specific steps that AHLs signal molecules with the specific variety and the specific concentration are added to a reactor containing AOB activated sludge, under the condition that a sufficient nitrogen source and oxygen are provided, a quorum-sensing system is enhanced, aerobiotic ammonia oxidizing bacterium gathering growth is promoted, and biological membranes or granular sludge is obtained. According to the method, the AOB physiology is adjusted and controlled on the aspect of the signal molecules, AOB gathering growth is enhanced, rapid and efficient enrichment of AOB is achieved, and the AOB is fixed in a qualified sense, so that losses are reduced, and time needed for AOB enrichment is shortened. A new thought is provided for rapid AOB enrichment through the method, the method is expected to be used for the biological nitrogen removal process, and important significance is provided for efficient and stable operation of the ammonia-nitrogen wastewater biological treatment process.

The invention provides a method for preparing surface enhanced Raman substrate, and relates to Raman spectrums. The method comprises the steps that a seed crystal growing method is used for synthetizing a gold nanorod solution, wherein gold nano seeds are synthetized first, then a growing solution is added, silver nitrate is used for adjusting the slenderness ratio of gold nanorods until reaction is completed, the needed gold nanorod solution is obtained, then centrifuging is conducted to remove supernatant liquor, signal molecules are added to be connected to a gold surface, then a polyethylene glycol dressing agent is added to induce the gold nanorods to conduct self-assembling, then a processed nano particle solution is stirred, a TEOS methanol solution is added for reacting, silicon dioxide layers are wrapped on the surfaces of the self-assembled gold nanorods, and the surface enhanced Raman substrate is obtained. Detecting can be directly conducted in the solution, due to self-assembling and the coupling effect of plasma on the surfaces of adjacent nano particles, the electromagnetic field between the nano particles is obviously enhanced, the Raman signals at the position are amplified in a quantity level mode, accordingly, the detecting sensitivity of surface enhanced Raman is greatly improved, and the stability is high.

The invention provides a DNA tetrahedral nano-structure signal probe. The signal probe is a DNA signal probe having a tetrahedral base, wherein the signal probe is formed by self-assembling single-stranded DNA of a DNA recognition sequence extending from 5' end and single-stranded DNA of three chains with 5' ends modified with labeled molecule; the DNA recognition sequence extends from a vertex of the tetrahedral structure, the DNA recognition sequence is complementarily combined with to-be-detected target DNA or miRNA, and other three vertexes, due to the labeled molecules, are specifically combined with modified signaling modules, so as to achieve multi-signal amplification. The signal probe disclosed by the invention greatly improves the sensitivity of the target DNA or miRNA subjected to electrochemical detection, and a limit of detection can be as low as 1fM.

The invention relates to methods of expanding, stimulating or activating T cells by modulating the spatial organization of signal molecules presented to the T cells.

A method and kit developed to identify substances that act as ligands, corepressors, coactivators, agonist and antagonists for cloned nuclear hormone receptors, as well as a test kit for use in the methods is provided herein. More specifically, the method involves expressing a nuclear hormone receptor, receptor heterodimer, and / or receptor homodimer, DNA encoding one or more signaling molecules and DNA encoding a marker, incubating the cells with a test substance, and identifying whether the test substance interacts with the receptor quantitatively or qualitatively by identifying the amount of marker and / or the proliferation of the cells.

The invention discloses a method for rapidly forming an anammox granule sludge, and belongs to the field of wastewater treatment. Proper amount of an inoculated sludge is added to an SBR (Sequencing Batch reactor), a nutrient solution containing ammonia nitrogen and nitrite is added, the concentrations of both ammonia nitrogen and nitrite in the nutrient solution are both 50 mg / L, an AHLs quorum sensing signal molecule at certain concentration is added into the nutrient solution in the SBR each day, the environment temperature is 32-33 DEG C, the pH value is 7.8-8.3, the DO concentration is 0.15 mg / L or lower, and batch cultivation is conducted. The time for forming the anammox granule sludge is short, and the effect of the granule sludge is good.

The invention discloses a chimeric antigen receptor containing a CD27 intracellular domain, a lentiviral vector and application thereof. The immune receptor tyrosine activation sequence motif of the chimeric antigen receptor containing CD27 intracellular domain contains CD27molecule intracellular signal domain and T cell costimulatory signal molecules. The CD27molecule intracellular signal domain is connected with costimulatory signals related to T cell activation, so that the in-vitro proliferation and apoptosis effect of T cells can be obviously enhanced, and the ratio of young sample Tscm cells (CD45RA+CD62L+) with in vivo therapeutic action and central memory cells (CD45RO+CD62L+) in CAR-T cells is increased; expression of IL-10 factors capable of restraining immunization is reduced, an obvious effect of improving the CAR-T (T cell chimeric antigen receptor) cellular therapy effect is achieved, and a new idea and a new selection are provided for the field of CAR-T cellular therapy.

The invention belongs to the technical field of biological nano-materials, and concretely relates to a near-infrared second window emission down-conversion nanometer fluorescence probe and a synthesismethod thereof. The fluorescence probe comprises an inert core layer, a down-conversion luminescence center shell layer, an energy transfer shell layer and an energy absorbing shell layer; the inertcore layer is used for adjusting the size of whole nano-crystals; the above down-conversion luminescence center is activated ion-doped rare earth nano-crystals; the energy transfer layer is used for transferring absorbed energy to the luminescence center, and the relaxation time of luminescence ions is extended or shortened by changing the thickness of the energy transfer layer in order to changethe fluorescence lifetime of the nano-crystal fluorescence probe, so the controllability and the adjustability of the fluorescence lifetime are achieved; and the energy absorbing layer is used for absorbing external excitation lights. The probe can be used for simultaneous in-situ detection of multiple signal molecules in deep tissues, further improves the detection sensitivity and the detection accuracy, and has broad application prospects in the fields of protein expression, high-throughput screening of biological samples and multi-channel biological detection.

A method of repairing diseased or dysfunctional organs or of establishing a model system of a disease state is provided. For repairing diseased organs, the method involves engraftment of cells from healthy tissue of the diseased or dysfunctional organ admixed with gel-forming biomaterials and nutrient medium, signaling molecules and extracellular matrix components that can be made insoluble rapidly upon transplantation to form a graft. In this way, the graft mimics the complexity of the native microenvironment with a minimum number of components that allow transplantation of cells to successfully engraft, expand and then rebuild part or the entirety of the diseased or dysfunctional organ. In the case of using grafting methods for establishing a disease model, diseased cells may be transplanted in the biomaterials and into experimental hosts.

The invention belongs to the technical field of photoelectrochemical sensors, and particularly relates to a carbon quantum dot-nanowire array-based cardiomyocyte signal molecule sensor based on sunlight drive and a preparation method thereof. The preparation method comprises the following steps: under a hydrothermal system, growing a titanium dioxide nanowire array on a conductive glass substrate; obtaining silane-functionalized nitrogen-doped carbon dots (N-CDots) at one step in situ through using a microwave method; then placing titanium dioxide nanowires processed by concentrated sulfuric acid into an N-CDot ethanol solution, adding stronger ammonia water, standing and reacting, and defining as N-CDot-TiO2. The photoelectric current of the N-CDot-TiO2 is improved by almost two times compared with that of pure TiO2, and the prepared photoelectric current on-off sensor can be used for dynamically detecting the content of H2S star molecules of cardiomyocytes in real time in situ. The carbon quantum dot-nanowire array-based cardiomyocyte signal molecule sensor is smart in design and wide in the source of raw materials, the preparation method is simple, environment-friendly, low in price, fast to response, wide in linear range, high in selectivity, and beneficial to popularization and application.

The invention relates to a difunctional tumor diagnosis reagent and a method thereof, and belongs to the nano-technological field, the biological bionic field, and the immunology and biomedicine crossing field. The invention especially provides the difunctional tumor diagnosis reagent which is composed of a protein shell for specifically identifying cancer tissues and / or cancer cells and an inorganic nanometer core having a peroxidase catalytic activity. The invention also provides a kit comprising the reagent, and a method for detecting tumors in an individual through using the reagent. The difunctional tumor diagnosis reagent and the method can be used for cancer screening, early-stage diagnosis, treatment monitoring, cancer cell metastasis analysis, postoperative recurrence evaluation or basic cancer researches. The difunctional tumor diagnosis reagent integrates two functions comprising tumor specific identification and coloration into one, and enables the tumor specific identification and the coloration to be completely in a one step manner without the marking of a secondary antibody, a tertiary antibody or HRP or a signal molecule, and the operation is simple and convenient.

The invention relates to bacillus pumillus with a quorum sensing system inhibiting effect. The preservation number of the bacillus pumillus is CCTCC M2013240. The invention also provides an application of the bacillus pumillus and a microbial preparation which takes the bacillus pumillus or bacillus pumillus fermentation liquid as an active component. The invention has the advantages that chromobacterium violaceum is used as a screening model, a T-shaped streaking method and a filter paper method are used for screening out a bacterial strain F3-1 which can suppress the generation of the purple pigment of the chromobacterium violaceum, test results show that the bacterial strain F3-1 can suppress the QS signal molecule of the chromobacterium violaceum, has the effect of suppressing bacterial colony sensing and can be used for preparing the microbial preparation capable of preventing and treating aquatic bacterial diseases, and the foundation is laid for further researching a new aquatic bacterial disease preventing and treating method from the point of an interference bacterial colony sensing system.