Mercury ion detection reagent and detection method

A technology for detecting reagents and mercury ions, applied in Raman scattering, material excitation analysis, etc., can solve problems such as laborious, incapable of direct detection, and difficult to meet environmental monitoring requirements

Active Publication Date: 2011-01-05
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are either relatively laborious and time-consuming, and require relatively complex instruments and skilled technical personnel to obtain reliable test results; or they are trace analysis methods, which are difficult to meet the current growing environmental monitoring requirements.
[0003] There are many kinds of metal ions in aqueous solution and their valence states are changeable. Conventional Raman analysis methods cannot directly detect them. Selective detection of a certain metal ion is even more challenging.
These limitations prevent conventional Raman analysis from becoming a ubiquitous analytical technique for the detection of metal ions

Method used

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  • Mercury ion detection reagent and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] a. Synthesis of silver nano-sol at room temperature (N.Leopold and B.Lendl.J.Phys.Chem.B., 2003,107:5723-5727): first add 5mL hydrochloric acid to a conical flask containing 84mL deionized water Hydroxyl ammonia (0.03mol / L) and 1mL NaOH (0.6mol / L) with vigorous stirring. Then add AgNO at a constant speed of 2-3 drops / second 3 (0.01mol / L) a total of 10mL. After continuing to stir for 1 hour, bright yellow AgNPs with a particle size of 35±8nm were obtained, which were used as the stock solution nanoparticle sol for future use.

[0032] b. Add 1 μL of aptamer solution (100 μmol / L) to 200 μL of fresh AgNPs and mix well. The sequence of the aptamer is: 5'-TAMRA-TTCTT TCTTCCCTTG TTTGTT-3'.

[0033] c. Add 5 μL spermine (100 μmol / L) to the above solution, and mix well (D.Graham, W.E.Smith, A.M.T.Linacre, C.H.Munro, N.D.Watson and P.C.White.Anal.Chem., 1997, 69: 4703- 4707).

[0034] d. Add a certain concentration of Hg to 90 μL sol respectively 2+ Solution 10μL, incubate...

Embodiment 2

[0040] During the implementation process, the silver sol synthesis method in Example 1 was used to obtain fresh silver sol, which was centrifuged, and a small amount of water was added to obtain AgNPs whose particle concentration was 2.5 times the initial concentration. Add 2.5 μL aptamer solution (100 μmol / L) to 200 μL fresh AgNPs and mix well. The sequence of the aptamer is the same as in Example 1. Add 10 μL of spermine (100 μmol / L) to the above solution and mix well. Add high concentration Hg to the above sol respectively 2+ solution, 1:1 (volume ratio) mixed, incubated for 5min. Use a glass capillary to absorb a little sol and collect Raman scattering signals. The experimental conditions are the same as in Example 1. at 1651cm -1 The intensity of the Raman peak is the standard peak for data processing. The example for Hg 2+ The detection limit is lower than 5nmol / L, for Hg 2+ The linear response range of concentration is 5-150nmol / L, and at the same time, it has h...

Embodiment 3

[0042] a. Same as Example 1; b. Add 1 μL of aptamer solution (100 μmol / L) to 200 μL of fresh AgNPs and mix well. The sequence of the aptamer is: 5'-TAMRA-TTTTTTTTTTTTTTTTTTTTTTTT-3'; c-g are the same as in Example 1. The example for Hg 2+ The detection limit is lower than 2nmol / L, for Hg 2+ The linear response range of concentration is 5-80nmol / L, and at the same time, it has high selectivity, and the whole detection process only takes 5-10 minutes.

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Abstract

The invention discloses a mercury ion detection reagent and a detection method. The reagent comprises nano sol, nucleic acid adaptors marked by Raman active molecules and a charge modifier. The detection method comprises the following steps of: firstly, synthesizing the nano sol, then adding a proper quantity of adaptors to a proper quantity of fresh nano sol and uniformly mixing; continuing to add a proper quantity of charge modifier and uniformly mixing; then adding a sample to be tested and incubating for 1-5 minutes; and finally, taking the nano sol to test a Roman scattering spectral signal and carrying out data processing. In the invention, based on the addition of Hg<2+>, the structures of the nano particle surface nucleic acid adaptors are changed, i.e. nano particle aggregation in the silver sol and surface enhanced Roman scattering signals of signal molecules are enhanced. In the invention, the SERS (Surface Enhanced Raman Scattering) technology is adopted, the detection limit to the Hg<2+> is at the nmol/L level, thus the invention has the advantages of high detection speed of the linear response range of Hg<2+> concentration and high selectivity and is suitable for the quick detection of trace Hg<2+> in environmental water.

Description

technical field [0001] The invention relates to a detection reagent and a detection method, in particular to a mercury ion detection reagent and a detection method. Background technique [0002] Common heavy metals are generally highly toxic. Excessive accumulation of heavy metals in the human body can affect the metabolism at the cellular level and the normal operation of organisms, causing irreversible damage to tissues and organs. As one of the most common heavy metal pollutants, mercury is discharged into the environment in large quantities through coal burning, metallurgy, volcanic eruption and waste disposal. Like other heavy metals, mercury cannot be degraded by microorganisms, on the contrary, it generates more toxic metal-organic compounds (such as methylmercury) under the action of aquatic microorganisms, and is enriched by thousands of times under the action of biomagnification in the food chain. collection, thereby causing great harm to ecosystems and human hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 陈令新王国庆李金花张卫卫
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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