21 results about "Methylmercury" patented technology

The invention belongs to the technical field of new materials, and particularly discloses nitrogen-rich activated carbon for soil mercury passivation and a preparation method and application thereof.The preparation method comprises the steps that dicyandiamide is subjected to a hydrothermal reaction to obtain a porous g-C3N4 precursor, then the porous g-C3N4 precursor and activated carbon are evenly mixed, and finally heat treatment is conducted to obtain the nitrogen-rich activated carbon. The prepared nitrogen-rich activated carbon can effectively remove dissolved mercury in a rice field. The cost is low, the preparation method is simple, and the prepared nitrogen-rich activated carbon is stable in structure, large in specific surface area, multiple in active sites and capable of effectively resisting interference of soluble organic matter and coexisting ions in soil, efficient fixation of biological available mercury in soil pore water is achieved, and generation of methyl mercury is reduced to the maximum extent.

The present disclosure provides a fungus and a microbial agent for treating mercury contamination, use thereof, a mercury removal method, and a method for identifying a fungus capable of treating mercury contamination, and relates to the technical field of biological mercury removal. The present disclosure provides a Metarhizium fungus and 8 species of non-Metarhizium fungi for treating mercury contamination, a method for identifying a fungus capable of treating mercury contamination, and enzymes for removing methylmercury and divalent mercury, which provides a genetic basis for treating mercury contamination with recombinant fungi or bacteria.

The application discloses an improved plant liquid culture medium, which comprises inorganic nutrients required by plant basic growth and does not comprise compounds containing an EDTA ligand skeleton and iodide. The application also discloses application of the improved plant liquid culture medium in mercury compound metabolism research. The application is based on the technical problem of finding that a culture medium substrate mediates abiotic transformation and causes error defects, and by eliminating the compounds containing the EDTA ligand skeleton and the iodide which induce abiotic transformation of mercury compounds, the application is applied to block the abiotic transformation path of methyl mercury in the mercury compound metabolism research, and eliminate the 'false positive' error in the metabolism research.

The present application relates to the technical field of food safety detection, and more particularly to a reagent tube fixing structure for pretreatment of methylmercury residue detection in oysters, comprising a box body and a box cover, a plurality of placing racks are arranged in the box body, the placing racks are rotatably installed in the box body, placing grooves are formed in the placing racks, reagent tubes are placed in the placing grooves, limiting bands are installed in the placing grooves through elastic assemblies, installation grooves are formed in the placing grooves, moving blocks are slidably installed in the installation grooves, first extension springs are symmetrically and fixedly installed on the moving blocks, and pressing plates are installed on the moving blocks through rotating assemblies. The reagent tubes are stably fixed on the placing racks through the cooperation of the elastic assemblies, the limiting bands, the first extension springs, the moving blocks, the rotating assemblies and the pressing plates. When the reagent box is moved, the reagent tubes in the reagent box can be effectively prevented from being bumped, the reagent tubes are better protected, the reagent tubes are prevented from being damaged, and losses are reduced.

The invention relates to the technical field of biological analysis, in particular to a method for analyzing methylmercury-tolerant key metabolites of tetrahymena thermophila. The method comprises the following steps: respectively culturing tetrahymena thermophila in a culture medium containing methyl mercury to respectively obtain experimental groups; culturing tetrahymena thermophila in a culture medium without methyl mercury to obtain a control group; then carrying out pretreatment on tetrahymena thermophila of the experimental group and the control group, then carrying out detection and quality control, respectively collecting data of metabolites of the experimental group and the control group, and then carrying out data processing; and finally, screening the differential metabolites of the experimental group and the control group, and then carrying out pathway enrichment analysis by adopting KEGG to obtain the change of the metabolic pathway related to the response of the tetrahymena thermophila to the methylmercury stress, and further selecting the key metabolite related to the metabolic pathway as the key metabolite related to the methylmercury thermophila to the tetrahymena thermophila.

The invention relates to the technical field of analysis by determining the property of methyl mercury, in particular to a method for rapidly detecting methyl mercury in sludge based on mass spectrometry. The method comprises the following steps: acquiring a target characteristic time period of a standard mass spectrum signal; obtaining the local form stability of each intrinsic mode component according to the change trend of each intrinsic mode component at different moments in the target characteristic time period and the moment distribution; combining the power spectrum density distribution of each intrinsic mode component at different frequencies to obtain the time-frequency joint saliency of each intrinsic mode component; obtaining the peak time alignment degree of each intrinsic mode component according to the position distribution of peak points of different intrinsic mode components in the target characteristic time period; and in combination with the peak distribution of each intrinsic mode component in the target characteristic time period, obtaining a wavelet correction denoising threshold value, and obtaining an intrinsic mode denoising component to carry out methyl mercury detection. According to the method, the wavelet denoising threshold is adaptively adjusted, the signal denoising effect is optimized, and the accuracy of methyl mercury detection is improved.

One embodiment of the present invention provides a peptide-based real-time proportional fluorescence sensor with excellent selectivity for methylmercury ions and mercury ions, and a method for detecting methylmercury and mercury ions using the same. According to one embodiment of the present invention, the peptide-based real-time proportional fluorescence sensor with excellent selectivity for methylmercury ions and mercury ions dissolves well in a pure aqueous solution (1% DMSO), is excited by visible light, and can recognize Hg(II) and methylmercury with high sensitivity through proportional fluorescence behavior. In particular, it exhibits a fast detection speed for methylmercury and has high water solubility, but this fluorescence sensor penetrates well into cells and can sensitize Hg(II) and methylmercury inside the cell through a change in the ratio of red and green fluorescence.

The disclosure relates to methods and processes for protecting food supply, organisms including fish and aquatic life, and plants from mercury accumulation by reducing the presence of methyl mercury in ecosystems, sediment, and pore water. The disclosure including treating sediment and / or pore water with an amendment that contains a sorbent and a halogen source, or a halogen containing sorbent.

The invention provides a method for enhancing methyl mercury degradation capacity of phytoplankton, and belongs to the field of biotechnology. The method for enhancing the methyl mercury degrading capacity of the phytoplankton comprises the step of enabling the phytoplankton to overexpress a methyl mercury lyase gene merB. The organic mercury lyase coded by the merB gene, namely the MerB protein, can decompose high-toxicity methyl mercury into low-toxicity Hg and methane (CH), so that the tolerance of phytoplankton expressing the merB gene to the methyl mercury can be enhanced. Meanwhile, the phytoplankton can enrich methyl mercury in the water environment, the phytoplankton serves as a primary producer in a food chain and is a source of substances and energy, the content of methyl mercury can be reduced from the source by reducing the methyl mercury in the phytoplankton, and therefore the ecological health risk that the methyl mercury is transmitted along the food chain is reduced. The mercury metabolic pathway of phytoplankton is enhanced through genetic engineering, and an efficient and sustainable method is provided for bioremediation of mercury pollution.

The invention provides a preparation method of a phenylboronic acid functionalized silicon quantum dot fluorescent probe and application of the phenylboronic acid functionalized silicon quantum dot fluorescent probe in detection of mercury ions and methyl mercury ions, and relates to the technical field of detection of heavy metal mercury ions and methyl mercury ions. In the presence of mercury ions (Hg < 2 + >) and methyl mercury ions (CH3Hg < + >), silicon point fluorescence is quenched. By combining ultraviolet portable equipment and combining a silicon puncture-Hg < 2 + > and silicon puncture-CH3Hg < + > system with RGB value acquisition and conversion of a smart phone platform, rapid and high-sensitivity detection of mercury ions and methyl mercury ions in an environmental water body is realized. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, rapid feedback, high sensitivity, good repeatability, high stability, strong accuracy and the like, can intuitively realize rapid determination of mercury ions and methyl mercury ions in a water sample in real time, and widens the application direction of silicon dots in the aspect of environmental detection.

The invention discloses a slow-release oxygen fertilizer as well as a preparation method and application thereof, and belongs to the technical field of slow-release fertilizers. The slow-release oxygen fertilizer comprises a buffer layer, an oxygen source layer and a coating layer from inside to outside, and all the layers are isolated by filling layers. Wherein the buffer layer comprises a compound fertilizer composed of urea phosphate and a potash fertilizer; the oxygen source layer comprises a chemical oxygen producer calcium peroxide; the coating layer comprises polymer resin polyacrylate; the filling layer comprises a filling agent. The slow-release oxygen fertilizer developed by the invention has multiple functions of slow-release oxygen, acid-base buffering, nutrient supply and the like. The fertilizer is also suitable for being applied to acid-base sensitive crops, can promote the growth of various crops, and is not easy to cause soil hardening and salinization after being applied for a long time. In addition, when the slow-release oxygen fertilizer is applied to the mercury-polluted soil, the slow-release oxygen fertilizer has a remarkable inhibition effect on dissolution of mercury and formation of methyl mercury in the soil, and has important significance in promoting safe production of food crops.

The present invention belongs to the field of detection and analysis technology, and discloses a mercury fluorescent probe based on carbazole boronic acid and a preparation method thereof. 9H-carbazole-3-boronic acid pinacol ester and compound Y (any one of KOH, K2CO3, Cs2CO3, NaH, triethylamine, DBU, DMAP, and DABCO) are dissolved in a polar aprotic solvent, and then alkylene oxide is added. After heating and stirring for a period of time, material A is obtained; the organic phase in material A is extracted with EA, and then compound B is obtained by column chromatography purification; compound B is dissolved with a solution of acetone / water or THF / water, and then a mixture of NaIO4 and CH3COONH4 or a mixture of NaIO4 and hydrochloric acid is added. After reacting for a period of time, the organic phase is extracted with EA, and then the fluorescent probe is obtained by column chromatography purification. The fluorescent probe provided by the present invention can be used for quantitative detection of mercury ions and methylmercury in water samples, and has good sensitivity and selectivity. The present invention further provides the application of two other compounds as fluorescent probes in mercury detection.

The application provides a biochar modifier for reducing plant mercury and soil methylmercury, and a preparation method and application thereof. The biochar modifier provided by the application comprises biochar loaded with selenium and phosphorus. The preparation method of the biochar modifier comprises the following steps: dipping biochar into a solvent containing a selenium modifier and a phosphorus modifier and reacting, and then performing solid-liquid separation on the reaction solution to obtain a solid phase; and the solid phase is dried to obtain the biochar modifier. The biochar modifier provided by the application has a remarkable effect of reducing plant mercury and soil methylmercury, has a simple preparation process, does not cause secondary selenium pollution, and is easy to popularize.

The invention relates to the technical field of biological analysis, in particular to a method for analyzing a methyl mercury tolerance key gene of tetrahymena thermophila. The method comprises the following steps: respectively culturing tetrahymena thermophila in a culture medium containing methyl mercury to respectively obtain experimental groups; culturing tetrahymena thermophila in a culture medium without methyl mercury to obtain a control group; carrying out pretreatment on the tetrahymena thermophila of the experimental group and the control group, then carrying out detection and quality control, respectively collecting RNA data of the experimental group and the control group, and then carrying out data processing; finally, screening differential genes of the experimental group and the control group, and then carrying out pathway enrichment analysis by adopting KEGG to obtain changes of related pathways of the tetrahymena thermophila in response to methylmercury stress, and further selecting pathway-related key genes as key genes of the tetrahymena thermophila in tolerance to methylmercury.

The application relates to the field of agricultural heavy metal microbial remediation, and discloses a new sulfate-reducing strain, a new sulfate-reducing strain Desulfovibrio sp.GLDM01 which has been preserved in the China Center for Type Culture Collection on March 9, 2026, and the preservation number is CCTCC NO:M 2026395. The new sulfate-reducing strain has the functions of adsorbing cadmium elements in soil and improving the ability of rice to enrich methylmercury, thereby providing new strain resources for farmland heavy metal remediation.

The application discloses a method for preparing high-purity vanadium pentoxide in a short process, and belongs to the technical field of non-ferrous hydrometallurgy. The method comprises the following steps: firstly, after pretreatment of vanadium-containing leaching solution, sodium sulfide and na-methylmercury sulfonate are added, aging is carried out, impurities are removed, hydrogen peroxide is added and heated, pH is adjusted, attapulgite is added, aluminum sulfate is added, aging and impurity removal are carried out; secondly, vanadium precipitation treatment is carried out, ammonium polyvanadate is washed, dried and calcined, and high-purity vanadium pentoxide with a grade of greater than or equal to 99.5% is obtained. In the application, the vanadium-containing leaching solution is used to produce high-purity vanadium pentoxide, the impurity removal process is advanced, high-purity vanadium pentoxide with a grade of greater than or equal to 99.5% can be prepared, and the vanadium recovery rate can reach 98%. The preparation process is greatly simplified, the production efficiency is high, the cost is reduced, the treatment time is short, energy saving and emission reduction and green production are beneficial, the production cost is reduced by 3000-5000 yuan / ton, the vanadium recovery rate can be increased by about 3%, the process is more environmentally friendly, the vanadium recovery rate is higher, and the production efficiency is also higher. The application is suitable for preparing high-purity vanadium pentoxide in a short process.

The invention discloses a remediation agent for heavy metal contaminated soil and a preparation method of the remediation agent. The remediation agent is prepared from the following raw materials: modified magnetic biochar, modified carboxymethyl cellulose, rhamnolipid and metarhizium anisopliae spore powder. The modified magnetic biochar is grafted with sulfydryl to rapidly capture Hg < 2 + >, the signal response layer expands in the flooding period to form a porous structure, and S < 2-> enters and is combined with Hg < 2 + > to form Hg < S > precipitate. In the draining period, oxygen enters, the signal response layer is densified, a physical barrier is formed, and the oxygen is prevented from entering to oxidize HgS. Modified carboxymethyl cellulose grafted gallic acid inhibits microorganisms from forming a biological membrane on the surface of the modified magnetic biochar, and polyethylene glycol diacrylate resists non-specific adsorption of hydrophobic organic macromolecules in the environment, so that pore channels of the modified magnetic biochar are kept unobstructed. Mercury and methyl mercury in the soil are converted into free Hg < 2 + > through rhamnolipid and metarhizium robertianum, and the capture rate of the modified magnetic biochar on the Hg < 2 + > is increased.

The invention relates to a preparation method of mesoporous silica pore channel confinement ferrous sulfide and application of the mesoporous silica pore channel confinement ferrous sulfide in mercury pollution remediation, ferrous sulfide is precipitated among pore channels of mesoporous silica nanoparticles by using a double-solvent method, and nano confinement ferrous sulfide is prepared. The mesoporous silica pore channel confinement ferrous sulfide can be used for removing mercury in environmental pollution, and has higher mercury saturation adsorption capacity and stronger mercury methylation inhibition effect. The confined ferrous sulfide can maintain good dispersibility due to the support of the mesoporous silicon pore skeleton, so that efficient fixation of mercury ions and effective control of methyl mercury pollution are maintained.

The invention discloses a methyl mercury in-situ sample injection device utilizing a gas chromatography gas circuit, the device utilizes the gas chromatography gas circuit, and a gas phase detection device comprises a purging module (a sample injection needle, a gas flowmeter, a sample injection bottle with a polytetrafluoroethylene inner pad, a pressure stabilizing valve and a chromatography air circuit); the trapping module comprises a trapping trap, a quartz conduit, a heating coil with the resistance of about 11 ohm, a temperature control system and a heat dissipation system; the chromatography and sample injection module is based on a column oven, a chromatographic column and a carrier gas flow path flow control system of an old gas chromatograph. According to the device, accurate detection of methyl mercury is realized through an idle steady flow and steady pressure system and a gas path system of old gas chromatography. A water sample standard addition recovery experiment and a biological sample standard substance both show that the result meets the American EPA1630 standard.

The invention relates to a bioremediation method for targeted lysis of mercury methylated bacteria by using a specific bacteriophage, which comprises the following steps: separating target bacteria from lake sediments polluted by mercury, screening bacteriophages capable of lysing the bacteria, culturing the bacteriophages and obtaining a high-concentration bacteriophage preparation, and applying the bacteriophage preparation to the sediments. Methylation of mercury is mainly mediated by specific bacteria (such as sulfate reducing bacteria and methanogens), and the bacteria exist in the sediment in a large number. By screening and culturing bacteriophages capable of effectively splitting the bacteria, the number of the bacteriophages can be remarkably reduced, and the generation of methyl mercury is further reduced. Experimental results show that after the bacteriophage is applied, the concentration of methyl mercury in sediment is remarkably reduced and reaches 60% or above, and the method has application potential in lake ecological restoration. The method has the advantages of simple operation, low cost, environmental protection and the like, and is suitable for wide water pollution treatment.