551 results about "Fluorescence sensor" patented technology

The invention relates to the field of physiological status detection, and provides a wearable microfluidic fluorescence sensor based on a Janus membrane for sweat analysis. The sensor comprises a Janus membrane-based micro-fluidic chip and a fluorescent sensing layer which are stacked, the Janus membrane-based micro-fluidic chip comprises a hydrophobic nanofiber membrane, a bottom polydimethylsiloxane layer, a hydrophilic nanofiber membrane and a top polydimethylsiloxane layer which are stacked, and the fluorescent sensing layer comprises three paper chips, the simultaneous fluorescence detection of chloride ions, glucose and pH of sweat is realized. The Janus membrane with the directional water conveying capacity is used as a substrate for preparing a micro-fluidic chip. In one aspect, sweat may be automatically delivered to an analysis zone along a designed path. On the other hand, sweat is transferred from a hydrophobic membrane close to the skin to a hydrophilic membrane, sweat accumulation is effectively avoided, and a comfortable skin microenvironment is promoted. A Janus membrane-based micro-fluidic chip is combined with fluorescence sensing, a micro-fluidic fluorescence sensor based on a Janus membrane is constructed, and simultaneous high-stability and high-accuracy analysis of multiple targets in human sweat is realized.

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention provides a D-A type organic fluorescent small molecule, a preparation method and application of the D-A type organic fluorescent small molecule in detection of nitrofuran antibiotics, and belongs to the technical field of fluorescent sensing. The D-A type organic fluorescent micromolecule is composed of 2-(2-aminophenyl) benzothiazole and an electron-donating group, the excitation spectrum of the D-A type organic fluorescent micromolecule is obviously overlapped with the absorption spectrum of nitrofuran antibiotics, and fluorescence quenching response can be shown through the inner filter effect; the trace nitrofuran antibiotics can be detected in a liquid phase in an ultrafast, high-sensitivity, excellent-selectivity and strong-anti-interference manner. According to the invention, a quantitative evaluation system can be constructed in combination with a smart phone, the RGB value of the image of the liquid-phase fluorescence sensor is output in real time, and rapid, accurate and visual quantitative detection of nitrofuran antibiotics in a real food sample or a water source is realized. In addition, the filter paper fluorescence sensor has the advantages of being portable and easy to prepare, and nitrofuran antibiotics can be rapidly identified on site in an actual scene.

The invention provides an oil spill monitoring system and method capable of automatically adjusting the angle of a light source. The oil spill monitoring system comprises an MCU controller, an ultraviolet light source arranged on the bottom surface of the oil spill monitoring equipment, a light source adjusting mechanism, two groups of fluorescence sensors, a distance sensor and an illumination intensity sensor, the illumination intensity sensor is located between the two fluorescence sensors, and the distance sensor is arranged between the two fluorescence sensors. The distance sensor is located between the light source and the illumination intensity sensor; the light source adjusting mechanism comprises a motor and a transmission mechanism, and the motor is connected with a lamp holder of the ultraviolet light source through the transmission mechanism and controls the ultraviolet light source to rotate; the MCU controller controls the motor and the light source, and in the monitoring process, the motor controls the emission angle of the ultraviolet light source to reach the deflection angle alpha. According to the invention, the illumination intensity is effectively utilized, so that the photoelectric sensor obtains the maximum illumination intensity; the light-emitting ultraviolet LED can be automatically adjusted according to the distance to the water surface, and the oil spilling condition on the water surface can be monitored more accurately.

The invention provides a flexible fluorescence sensor as well as a preparation method and application thereof. The flexible fluorescence sensor comprises TCTG particles and dual-network hydrogel, the TCTG particles are distributed in network pores of the dual-network hydrogel to form functional gel, the TCTG particles are formed by Ti3C2TxMXene nanosheets and graphene quantum dots, the dual-network hydrogel is formed by acrylamide and sodium carboxymethyl cellulose, and the TCTG particles are distributed in the network pores of the dual-network hydrogel to form functional gel. The functional gel is soaked in a hydrochloric acid glycol solution for treatment. The flexible fluorescence sensor provided by the invention can integrate the structural advantages of all the components, so that a synergistic coupling effect is possibly generated, a fluorescence detection function with excellent mechanical strength, frost resistance and high sensitivity is integrated into a multifunctional system, the food spoilage condition can be reliably monitored in real time under a harsh environmental condition, and the application prospect is wide. And health and economic risks caused by occupational exposure and environmental emission in an aquatic product supply chain are solved.

The invention discloses a shrimp pond water quality prediction method based on a deep echo state network, and relates to the technical field of shrimp pond water quality prediction, and the method comprises the following steps: deploying an underwater fluorescence sensor in a shrimp pond culture water body to detect a fluorescence response signal of algae, and obtaining algae concentration data in the water body in real time based on the fluorescence signal intensity; dynamic changes of algae in the prawn pond water are monitored in real time; and performing time sequence alignment on the collected algae concentration data, and constructing an algae concentration time sequence data map based on the aligned time sequence data. According to the method, fluorescence sensor data and a short-time feature extraction mechanism are fused, a deep echo state network is combined to model an algae burst trend, and early warning can be realized before red tide outbreak; and the abnormal area monitoring precision is improved by enhancing a sampling mechanism, the problem of slow recognition of emergencies by a traditional model is effectively solved, and the aquaculture water quality risk perception and intelligent management capability is remarkably improved.

The invention belongs to the technical field of fluorescence detection, and provides a dual-emission carbon dot, a visual hydrogel sensing platform prepared from the dual-emission carbon dot and application of the dual-emission carbon dot in order to solve the problem that field real-time detection cannot be realized in current tetracycline detection. The dual-emission fluorescent carbon dots DE-CDs are synthesized by dissolving toluene diisocynate, fennel and m-phenylenediamine (m-PD) in ultrapure water to serve as precursors, and reacting for 6 hours by controlling the reaction temperature to be 220 DEG C through a one-pot hydrothermal method. Two emission wavelengths (432 nm and 515 nm) are shown under different excitation wavelengths. A fluorescence sensor with double response signals is constructed and used for detecting tetracycline. The sensor shows a very strong index relationship in the range of tetracycline of 0-118 [mu] M, and the detection limit is 53.4 nM. A fluorescent hydrogel detection platform integrated with a smart phone is established, and rapid, portable and on-site detection of tetracycline in a water sample is successfully realized. And an effective strategy is provided for combination of laboratory precision testing and on-site rapid testing.

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The present invention provides a method for preparing a fluorescent sensor array for detecting heavy metal ions, the method comprising: mixing a cetylpyridinium chloride solution with a sodium hydroxide solution, then performing water bath ultrasound, purifying with dichloromethane, and then dialyzing with a dialysis bag to obtain a CPC-CDs solution; adding heavy metal ion solutions of different concentrations to CPC-CDs / XO sensing solutions under two groups of pH environments to obtain a fluorescent sensor array. Also provided is an application for detecting heavy metal ions Cr 6+ , Fe 2+ , Fe 3+ , and Hg 2+ The present invention simplifies the array construction steps by adjusting the pH environment of the system, and effectively improves the lack of affinity between carbon dots and heavy metal ions by adding xylenol, and can simultaneously detect multiple metal ions (Cr 6+ , Fe 2+ , Fe 3+ , and Hg 2+ ), with high sensitivity and good selectivity, is used for the detection of heavy metals in the environment.

The invention discloses a method for distinguishing similar antibiotics by using a fluorescent sensing array based on YOLOv5 target recognition. The method comprises the following steps: S1, preparing GMP-Tb solid powder; s2, mixing the GMP-Tb solid powder with a rhodamine B solution in proportion to obtain a multi-proportion fluorescent probe; s3, mixing antibiotics with different concentrations with the multi-proportion fluorescent probe to obtain a fluorescent sensing array; s4, collecting color images in the fluorescent sensing array and dividing the color images into a training set and a test set; s5, constructing an OLOv5 optic nerve model, inputting the acquired color image into a YOLOv5 optic nerve model, and outputting a feature value of the acquired color image; s6, inputting the characteristic value of the color image into a classifier model for prediction classification to obtain a distinguishing result; and S7, inputting a color image formed by mixing the to-be-detected antibiotic and the multi-proportion fluorescent probe into the YOLOv5 optic nerve model to obtain a characteristic value of the color image, and then outputting a distinguishing result through the classifier model.

The present invention combines a fluorescence sensor with an array to provide a fluorescence sensing array detection platform and a detection method for multiple plasticizers. The fluorescence sensing array detection platform includes a B-doped graphene quantum dot solution using N,N-dimethylformamide as a solvent and a B-GQDs solution using cyclohexane as a solvent; the fluorescence intensity of the B-GQDs solution with DMF as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 391 nm, 413 nm, and 434 nm are used as array points 1, 2, and 3; the fluorescence intensity of the B-GQDs solution with CH as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 386 nm, 408 nm, and 430 nm are used as array points 4, 5, and 6; thus, a 3×2 fluorescence sensor array is constructed. The detection method using the fluorescence sensor array of the present invention has simple steps, and has satisfactory specificity, reproducibility, stability and practicability, and has application potential.

There is provided an optical measurement device including a spectral optical system that disperses fluorescence emitted from a biological specimen, and an image sensor that receives the fluorescence dispersed by the spectral optical system and generates fluorescence information. The image sensor is divided into a plurality of regions that receive different wavelength components of the fluorescence and generate fluorescence information for each of the wavelength components.

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The invention relates to the technical field of carbon-nitrogen ratio detection, in particular to a sweet potato carbon-nitrogen ratio detection method and system based on a multispectrum and fluorescence sensor, and the method specifically comprises the following steps: constructing a weight coefficient based on the correlation between the leaf carbon-nitrogen ratio in each growth stage and the reflectivity of each wavelength, and combining the multispectrum and fluorescence spectrum of each leaf to obtain a weight coefficient; acquiring a coupling characteristic value; correcting the coupling characteristic value according to the soil conductivity; analyzing the multi-spectral characteristics and the fluorescence spectrum characteristics of the sweet potatoes, and constructing a multi-spectral index and a fluorescence spectrum index; predicting the carbon-nitrogen ratio of the to-be-detected sweet potato leaf based on the features of the leaf, and calculating the carbon-nitrogen ratio of the to-be-detected sweet potato leaf by combining the difference between the feature vectors of the to-be-detected sweet potato leaf and the leaves in all growth stages and introducing the carbon-nitrogen ratio of the previous growth stage; certain continuity and inertia of the change of the carbon-nitrogen ratio are considered, so that the method adapts to the change of the sweet potatoes in different growth stages, and the detection precision of the carbon-nitrogen ratio is improved.

The invention discloses a near-infrared fluorescence sensor, a near-infrared fluorescence sensor array and a preparation method and application of the near-infrared fluorescence sensor array, and belongs to the technical field of biological medicine. The composite material comprises a single-walled carbon nanotube, a single-stranded DNA (deoxyribonucleic acid) and hydrogel, the single-stranded DNA is connected and modified on the surface of the single-walled carbon nanotube, and the single-walled carbon nanotube modified with the single-stranded DNA is fixed in the hydrogel. Bacterial metabolites enter the hydrogel and influence the structure of the single-stranded DNA, so that the fluorescence intensity of the single-walled carbon nanotube is influenced. A plurality of near-infrared fluorescence sensors form an array to quickly capture component change caused by growth of bacteria in an object to be detected, and respond to fluorescence signal change, so as to visualize whether the components change or not. Whether bacteria grow or not is judged through response information of the multiple sensors, so that the bacteria are rapidly recognized, and the method has the advantages of being high in sensitivity, short in response time and small in needed sample size.

The invention belongs to the technical field of material chemistry and sewage detection, and particularly relates to a nanofiber for detecting Cu < 2 + > as well as a preparation method and application thereof. The invention provides a preparation method of a 1, 4-DHAQ (at) GEL-PCL nanofiber membrane, which comprises the following steps: dissolving gelatin and polycaprolactone in an organic solvent to obtain a substrate solution, and putting the substrate solution into 1, 4-dihydroxy anthraquinone for electrostatic spinning to obtain the nanofiber membrane. The nanofiber membrane can be used for preparing a solid fluorescence sensor for detecting Cu < 2 + > in a water body. The nanofiber membrane is prepared by only one step, the raw materials are green, environment-friendly, economical and easy to obtain, and the treatment process is simple; the original performance can be maintained and stored for six months under the normal-temperature dark condition; the prepared fluorescence sensor is short in incubation time, suitable for rapid detection of large-batch samples, high in sensitivity and good in stability; the prepared fluorescence sensor is a solid sensor and is convenient to carry. The sensor is resistant to interference of various ions, good in specificity and suitable for Cu < 2 + > detection in various wastewater treatment fields.

The invention relates to the field of molecular biology and clinical medicine, and relates to a double-split aptamer probe and application thereof. The double-split aptamer probe comprises two split aptamers, namely a split aptamer 1 and a split aptamer 2, the split aptamer 1 comprises an aptamer fragment 1 and a split initiator 1, the split aptamer 2 comprises an aptamer fragment 2 and a split initiator 2, and the two aptamer fragments are from a complete aptamer sequence A. The invention further discloses a preparation method of the double-split aptamer probe. The invention proves that DNA (deoxyribonucleic acid) can be successfully self-assembled in a solution to form a fluorescence tree with larger molecular weight under the triggering of a target, so that signal amplification is realized. The constructed sandwich-type fluorescence sensor can be successfully applied to Ade detection, in the concentration range of 0.01-100 ng / mL, the detected fluorescence intensity is enhanced along with increase of the concentration of Ade, LOD is not higher than 5.23 pg / mL, the detection time is not longer than 30 min, and the sandwich-type fluorescence sensor has the advantages of being high in sensitivity and short in detection time.

The invention belongs to the technical field of nano material detection, and particularly discloses a method for detecting Cr (VI) by using a double-lanthanide metal organic framework Eu / Tb MOF ratio fluorescence sensor, which comprises the following steps: screening and preparing a lanthanide metal organic framework Eu / Tb MOF using 1, 3, 5-dicarboxyphenylboronic acid as a ligand; and 2, detecting Cr (VI) by using an Eu / Tb MOF ratio fluorescence sensor. The Cr (VI) enables the fluorescence absorption peak of the Eu / Tb MOF to be quenched at 545nm and the fluorescence absorption peak of the Eu / Tb MOF to be almost unchanged at 618nm, so that the Eu / Tb MOF ratio fluorescence sensor is constructed to detect the Cr (VI), and the concentration of the Cr (VI) in a to-be-detected sample is judged through fluorescence intensity, color change and CIE chromaticity diagram degree. The Eu / Tb MOF ratio fluorescence sensor can generate more color intervals, Cr (VI with different concentrations can be detected more accurately, and the sensitivity is improved. The method disclosed by the invention has the characteristics of low cost, high response speed, low detection limit and high sensitivity and selectivity.

The invention discloses a miRNA-SNPs fluorescent sensor based on PfAgo protein programmable nuclease activity and a sensing method and application of the miRNA-SNPs fluorescent sensor. The fluorescent sensor comprises a chain A, a chain B, SplintR ligase, FQ and PfAgo nuclease. The chain A and the chain B generate a chain AB complementary to the miRNA to be detected through a base complementary pairing principle and the action of SplintR ligase; the AB chain excites PfAgo nuclease to perform targeted cleavage on an FQ single chain, so that a fluorophore FAM on the FQ single chain is separated from a quenching group BHQ1. According to the sensor and the sensing method thereof disclosed by the invention, zero background and accurate quantification of miRNA with mutated single basic group can be realized. The invention provides a simple, rapid and ultra-sensitive miRNA-SNPs accurate quantification strategy, and provides a powerful and universal detection platform for prognosis of human cancers related to miRNA related single nucleotide polymorphism.

The invention relates to the technical field of dispensing machines, in particular to an online dispensing visual recognition device. According to the technical scheme, a conveyor belt is installed on a machine body, and a machine head is installed on one side of the machine body through a mechanical arm; the machine head is provided with a base plate, a fluorescent sensor is installed on the surface of one side of the base plate, an industrial camera is installed on the surface of the side, away from the fluorescent sensor, of the base plate, a protective cover is installed on the portion, located on the outer side of the industrial camera, of the base plate, and a laser distance measuring sensor is installed on the end face of one side of the base plate. A dispensing head is installed on the end face of the side, away from the laser distance measuring sensor, of the base plate and provided with two feeding connectors, and the two feeding connectors communicate with glue and fluorescent agents through guide pipes correspondingly. According to the invention, visual detection of the glue coating position is realized, and the position and the coverage range of the glue are further confirmed through fluorescence detection. And the identification precision and reliability of the coating quality of the transparent glue are remarkably improved by a dual detection mechanism.

A pig for use in a pipeline is provided for determining the material of the pipeline in the context of an inline inspection. The pig includes a position determination unit and at least one braking arrangement for immobilizing the pig at a certain position in the pipeline. The pig also includes an X-ray fluorescence sensor.

The invention relates to a fluorine-containing polymer of a specific mononitroalkylbenzene aromatic hydrocarbon explosive tracer as well as a preparation method and application of the fluorine-containing polymer, and belongs to the technical field of fluorescent probes. The method specifically comprises the step of synthesizing a polymer with fluorine atoms as a solid fluorescence sensor probe. The probe realizes the detection of explosives through the interaction between fluorine atoms and nitryl and by doping CdSe / ZnS quantum dots. Based on the fluorine-containing polymer, aromatic alkyl hydrocarbon explosives (tracers) containing a single nitro group can be specifically recognized. Quantum dots are blended with the fluorine polymer, the quantum dots as a nano material are mixed and dispersed with the polymer to form a nanofiber membrane, and the fluorescence of the fluorine-containing quantum dot polymer is changed by further utilizing the interaction between fluorine and silicon atoms and nitro aromatic hydrocarbon, so that the fluorescence of the fluorine-containing quantum dot polymer is changed. Therefore, the specific detection-fluorescence rise of the mononitroalkane explosive is realized (fluorescence decline caused by interference such as light blocking is avoided).

The invention relates to an Eu-NH2BDC / OX MOF material as well as a preparation method and application thereof, and belongs to the technical field of fluorescent materials. According to the invention, 2-fluorobenzoic acid is used as a core cluster structure guiding agent, oxalic acid and 2-aminoterephthalic acid are used as organic ligands, and the Eu-NH2BDC / OX MOF material is synthesized based on a double-ligand strategy. The invention relates to application as a sulfur dioxide ratio fluorescence sensor, application in detection of sulfur dioxide and derivatives thereof in food, and development of fluorescence detection test paper for sulfur dioxide and derivatives thereof. The material has the dual-wavelength emission characteristic, the light emitting color changes from red to blue along with the SO3 < 2-> concentration change, visual inspection is facilitated, and the material has the advantages of quick response, high stability and high sensitivity; as a sulfur dioxide ratiometric fluorescence sensor, high-sensitivity and anti-interference detection on SO3 < 2-> can be realized, and the precision requirement of food safety detection can be met; the fluorescence detection test paper prepared from the Eu-NH2BDC / OX MOF material is simple, convenient and rapid to apply. The method realizes visual rapid detection of sulfur dioxide and derivatives thereof, and has important application value and market potential.

The invention relates to the field of sewage treatment, in particular to a reverse concurrent flow air floatation process system which comprises a dosing area, a reverse contact area, a concurrent contact area, a separation area and a clear liquid area which are arranged in an air floatation device box body, a sewage inlet and a dosing port are formed in the bottom of the dosing area, and an electric valve is arranged on the dosing port; a stirrer and a fluorescence sensor are arranged in the dosing area; a reverse flow dissolved air releaser is arranged at the bottom of the reverse contact area, a parallel flow dissolved air releaser is arranged at the bottom of the parallel contact area, and high-pressure nozzles are correspondingly arranged above the reverse flow dissolved air releaser and the parallel flow dissolved air releaser; a reciprocating device is arranged above the top of the separation area; and an oil skimming plate device capable of moving up and down is arranged on the reciprocating device. The medicine adding amount can be automatically controlled, and medicine liquid is evenly mixed and stirred; bubble flocs can be filtered when the moving trolley moves left and right; meanwhile, high-pressure water is sprayed to the surface of the dissolved air releaser from the high-pressure spray head, so that the dissolved air releaser is prevented from being blocked.

The invention discloses a fluorescence sensor for detecting thromboregulatory protein as well as a preparation method and a use method of the fluorescence sensor. The preparation method comprises the following steps: S1, adding a capture antibody of the thromboregulatory protein into an elisa plate; s2, adding the prepared bovine serum albumin; s3, adding prepared thrombus regulatory protein solutions with different concentrations; s4, adding a prepared detection antibody modified with alkaline phosphatase and used for detecting thromboregulatory protein; s5, adding a p-nitrophenyl disodium phosphate solution, and fully reacting; s6, a NO2-Eu-MOF nanosheet solution is prepared; s7, preparing a 1, 4-benzene diphosphonic acid solution; s8, adding a 1, 4-benzene diphosphonic acid solution into the NO2-Eu-MOF nanosheet solution, and detecting a fluorescence signal; and S9, adding the solution obtained in the step S8 into the solution obtained in the step S5, detecting the fluorescence intensity, and constructing a fluorescence intensity-concentration working curve. According to the invention, the concentration of thromboregulatory protein in the sample can be quantitatively analyzed.

The invention relates to the technical field of sensor preparation and antibiotic detection, in particular to a preparation method of a dual-emission fluorescence sensor, a product and application of the dual-emission fluorescence sensor. The preparation method of the dual-emission fluorescence sensor comprises the following steps: dissolving 1, 3, 5-benzene tricarboxylic acid and melamine in an organic solvent to prepare a suspension; the suspension liquid is subjected to a first heating reaction and then mixed with zinc salt to be subjected to a second heating reaction, and Zn-MBTC is obtained; the preparation method comprises the following steps: dissolving Zn-MBTC and Ru (bpy) 3 < 2 + > in water, carrying out ultrasonic treatment and oscillation, and then washing, centrifuging and drying to obtain the dual-emission fluorescence sensor. The dual-emission fluorescence sensor prepared by the method has the advantages of high sensitivity and high detection speed when being used for detecting four tetracycline antibiotics, namely doxycycline, tetracycline, oxytetracycline and gold tetracycline, and the detection limit is as low as 0.013 mu mol / L.

The invention relates to the technical field of fluorescence detection, in particular to a cadmium-metal-based fluorescent metal organic framework material, a preparation method of the cadmium-metal-based fluorescent metal organic framework material, a fluorescent sensor array and application of the cadmium-metal-based fluorescent metal organic framework material. Fluorescent probe molecules with different proportions can be obtained by changing the doping concentration of rhodamine B, the RhB (at) MOF has an emission peak of Cd-MOF at 430nm and an emission peak of rhodamine B at 570nm, and when the material is in contact with different benzene series solvents, the relative emission intensity of host-guest emission peaks is almost constant, and the material is unique for each RhB (at) MOF, so that the fluorescent probe can be used for detecting the fluorescence of the RhB (at) MOF. Benzene, toluene, xylene and ethylbenzene are specifically distinguished by using different relative emission ratios of host and guest emission peaks, a fluorescent sensing array is constructed to identify benzene series, and fluorescent probe molecules can be prepared into a fluorescent film to realize portable detection.

The invention relates to a nano-enzyme-based ratiometric fluorescence sensing platform for determining total antioxidant capacity and application thereof, and belongs to the technical field of preparation of fluorescence sensors. The ratio fluorescence sensing platform provided by the invention comprises MnSiO3 with oxidase-like activity, MnSiO3 with oxidase-like activity and MnSiO3 with oxidase-like activity, the invention relates to two fluorescent substrates with opposite responses. According to the ratio fluorescence sensing platform disclosed by the invention, the ratio fluorescence sensor is constructed by utilizing two fluorescent substrates, namely nano-enzyme MnSiO3 with oxidase-like activity and SC / OPD with opposite response, and is used for detecting TAC, so that the detection of three different antioxidants is realized. The ratio fluorescence sensing platform provided by the invention has the advantages of strong anti-interference capability and high sensitivity. The ratio fluorescence sensing platform has oxidase-like properties, does not introduce hydrogen peroxide, and has small interference on experiments.

The utility model provides an image acquisition device, an endoscope and a medical instrument, and the image acquisition device comprises a first image sensor; a second image sensor; the beam splitter prism is located on the light propagation path and is provided with a first incident plane and a first plane, and the first plane is opposite to the first incident plane; the first plane is provided with a semi-reflecting and semi-transmitting film, and the semi-reflecting and semi-transmitting film is configured to selectively divide the composite light incident to the beam splitter prism into reflected light and transmitted light according to the wavelength; the beam splitter prism is configured to guide the reflected light to the first image sensor; wherein the reflected light is visible light, and the first image sensor is a white light sensor; the transmission light is near-infrared light, and the second image sensor is a fluorescence sensor; and a reflecting prism configured to receive the transmitted light and guide the transmitted light to the second image sensor. The composite light can be split according to the wavelengths of different light beams in the composite light, the structure of the image acquisition device is simplified, and the production cost is reduced.

The invention discloses application of a ratiometric fluorescence sensor based on a fluorescein isothiocyanate labeled metal organic framework in escherichia coli detection. The method comprises the following steps: synthesizing ZTMs by taking TPPS as a ligand, and then fully incubating with FITC to prepare ZTMs (at) FITC; according to the present invention, Escherichia coli is used to reduce Cu < 2 + > to Cu < + >, such that the fluorescence intensity of ZTMs (at) FITC is changed, the fluorescence intensity of the emission peak at the 515 nm position is reduced, the fluorescence intensity of the emission peak at the 683 nm position is increased, and the color of the solution is observed to gradually change from yellow to orange and finally to red under the 365 nm ultraviolet light; and rapid and sensitive detection of the escherichia coli is realized through the ratio of the fluorescence intensities of the two emission peaks. According to the detection method, the accuracy of a detection result is improved, errors caused by environment, instruments and manual operation can be avoided, professional operators are not needed, the method has the advantages of being rapid, simple and independent of expensive instruments and equipment, and the detection cost is reduced.