412 results about "Fluorescence sensor" patented technology

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention provides a D-A type organic fluorescent small molecule, a preparation method and application of the D-A type organic fluorescent small molecule in detection of nitrofuran antibiotics, and belongs to the technical field of fluorescent sensing. The D-A type organic fluorescent micromolecule is composed of 2-(2-aminophenyl) benzothiazole and an electron-donating group, the excitation spectrum of the D-A type organic fluorescent micromolecule is obviously overlapped with the absorption spectrum of nitrofuran antibiotics, and fluorescence quenching response can be shown through the inner filter effect; the trace nitrofuran antibiotics can be detected in a liquid phase in an ultrafast, high-sensitivity, excellent-selectivity and strong-anti-interference manner. According to the invention, a quantitative evaluation system can be constructed in combination with a smart phone, the RGB value of the image of the liquid-phase fluorescence sensor is output in real time, and rapid, accurate and visual quantitative detection of nitrofuran antibiotics in a real food sample or a water source is realized. In addition, the filter paper fluorescence sensor has the advantages of being portable and easy to prepare, and nitrofuran antibiotics can be rapidly identified on site in an actual scene.

The invention provides an oil spill monitoring system and method capable of automatically adjusting the angle of a light source. The oil spill monitoring system comprises an MCU controller, an ultraviolet light source arranged on the bottom surface of the oil spill monitoring equipment, a light source adjusting mechanism, two groups of fluorescence sensors, a distance sensor and an illumination intensity sensor, the illumination intensity sensor is located between the two fluorescence sensors, and the distance sensor is arranged between the two fluorescence sensors. The distance sensor is located between the light source and the illumination intensity sensor; the light source adjusting mechanism comprises a motor and a transmission mechanism, and the motor is connected with a lamp holder of the ultraviolet light source through the transmission mechanism and controls the ultraviolet light source to rotate; the MCU controller controls the motor and the light source, and in the monitoring process, the motor controls the emission angle of the ultraviolet light source to reach the deflection angle alpha. According to the invention, the illumination intensity is effectively utilized, so that the photoelectric sensor obtains the maximum illumination intensity; the light-emitting ultraviolet LED can be automatically adjusted according to the distance to the water surface, and the oil spilling condition on the water surface can be monitored more accurately.

The invention provides a flexible fluorescence sensor as well as a preparation method and application thereof. The flexible fluorescence sensor comprises TCTG particles and dual-network hydrogel, the TCTG particles are distributed in network pores of the dual-network hydrogel to form functional gel, the TCTG particles are formed by Ti3C2TxMXene nanosheets and graphene quantum dots, the dual-network hydrogel is formed by acrylamide and sodium carboxymethyl cellulose, and the TCTG particles are distributed in the network pores of the dual-network hydrogel to form functional gel. The functional gel is soaked in a hydrochloric acid glycol solution for treatment. The flexible fluorescence sensor provided by the invention can integrate the structural advantages of all the components, so that a synergistic coupling effect is possibly generated, a fluorescence detection function with excellent mechanical strength, frost resistance and high sensitivity is integrated into a multifunctional system, the food spoilage condition can be reliably monitored in real time under a harsh environmental condition, and the application prospect is wide. And health and economic risks caused by occupational exposure and environmental emission in an aquatic product supply chain are solved.

The invention discloses a shrimp pond water quality prediction method based on a deep echo state network, and relates to the technical field of shrimp pond water quality prediction, and the method comprises the following steps: deploying an underwater fluorescence sensor in a shrimp pond culture water body to detect a fluorescence response signal of algae, and obtaining algae concentration data in the water body in real time based on the fluorescence signal intensity; dynamic changes of algae in the prawn pond water are monitored in real time; and performing time sequence alignment on the collected algae concentration data, and constructing an algae concentration time sequence data map based on the aligned time sequence data. According to the method, fluorescence sensor data and a short-time feature extraction mechanism are fused, a deep echo state network is combined to model an algae burst trend, and early warning can be realized before red tide outbreak; and the abnormal area monitoring precision is improved by enhancing a sampling mechanism, the problem of slow recognition of emergencies by a traditional model is effectively solved, and the aquaculture water quality risk perception and intelligent management capability is remarkably improved.

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The invention discloses a method for distinguishing similar antibiotics by using a fluorescent sensing array based on YOLOv5 target recognition. The method comprises the following steps: S1, preparing GMP-Tb solid powder; s2, mixing the GMP-Tb solid powder with a rhodamine B solution in proportion to obtain a multi-proportion fluorescent probe; s3, mixing antibiotics with different concentrations with the multi-proportion fluorescent probe to obtain a fluorescent sensing array; s4, collecting color images in the fluorescent sensing array and dividing the color images into a training set and a test set; s5, constructing an OLOv5 optic nerve model, inputting the acquired color image into a YOLOv5 optic nerve model, and outputting a feature value of the acquired color image; s6, inputting the characteristic value of the color image into a classifier model for prediction classification to obtain a distinguishing result; and S7, inputting a color image formed by mixing the to-be-detected antibiotic and the multi-proportion fluorescent probe into the YOLOv5 optic nerve model to obtain a characteristic value of the color image, and then outputting a distinguishing result through the classifier model.

The present invention combines a fluorescence sensor with an array to provide a fluorescence sensing array detection platform and a detection method for multiple plasticizers. The fluorescence sensing array detection platform includes a B-doped graphene quantum dot solution using N,N-dimethylformamide as a solvent and a B-GQDs solution using cyclohexane as a solvent; the fluorescence intensity of the B-GQDs solution with DMF as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 391 nm, 413 nm, and 434 nm are used as array points 1, 2, and 3; the fluorescence intensity of the B-GQDs solution with CH as the solvent at the fluorescence characteristic peak obtained under ultraviolet light excitation at a wavelength of 350 nm, and the fluorescence data at 386 nm, 408 nm, and 430 nm are used as array points 4, 5, and 6; thus, a 3×2 fluorescence sensor array is constructed. The detection method using the fluorescence sensor array of the present invention has simple steps, and has satisfactory specificity, reproducibility, stability and practicability, and has application potential.

There is provided an optical measurement device including a spectral optical system that disperses fluorescence emitted from a biological specimen, and an image sensor that receives the fluorescence dispersed by the spectral optical system and generates fluorescence information. The image sensor is divided into a plurality of regions that receive different wavelength components of the fluorescence and generate fluorescence information for each of the wavelength components.

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The invention relates to the technical field of carbon-nitrogen ratio detection, in particular to a sweet potato carbon-nitrogen ratio detection method and system based on a multispectrum and fluorescence sensor, and the method specifically comprises the following steps: constructing a weight coefficient based on the correlation between the leaf carbon-nitrogen ratio in each growth stage and the reflectivity of each wavelength, and combining the multispectrum and fluorescence spectrum of each leaf to obtain a weight coefficient; acquiring a coupling characteristic value; correcting the coupling characteristic value according to the soil conductivity; analyzing the multi-spectral characteristics and the fluorescence spectrum characteristics of the sweet potatoes, and constructing a multi-spectral index and a fluorescence spectrum index; predicting the carbon-nitrogen ratio of the to-be-detected sweet potato leaf based on the features of the leaf, and calculating the carbon-nitrogen ratio of the to-be-detected sweet potato leaf by combining the difference between the feature vectors of the to-be-detected sweet potato leaf and the leaves in all growth stages and introducing the carbon-nitrogen ratio of the previous growth stage; certain continuity and inertia of the change of the carbon-nitrogen ratio are considered, so that the method adapts to the change of the sweet potatoes in different growth stages, and the detection precision of the carbon-nitrogen ratio is improved.

The invention discloses a near-infrared fluorescence sensor, a near-infrared fluorescence sensor array and a preparation method and application of the near-infrared fluorescence sensor array, and belongs to the technical field of biological medicine. The composite material comprises a single-walled carbon nanotube, a single-stranded DNA (deoxyribonucleic acid) and hydrogel, the single-stranded DNA is connected and modified on the surface of the single-walled carbon nanotube, and the single-walled carbon nanotube modified with the single-stranded DNA is fixed in the hydrogel. Bacterial metabolites enter the hydrogel and influence the structure of the single-stranded DNA, so that the fluorescence intensity of the single-walled carbon nanotube is influenced. A plurality of near-infrared fluorescence sensors form an array to quickly capture component change caused by growth of bacteria in an object to be detected, and respond to fluorescence signal change, so as to visualize whether the components change or not. Whether bacteria grow or not is judged through response information of the multiple sensors, so that the bacteria are rapidly recognized, and the method has the advantages of being high in sensitivity, short in response time and small in needed sample size.

The invention belongs to the technical field of nano material detection, and particularly discloses a method for detecting Cr (VI) by using a double-lanthanide metal organic framework Eu / Tb MOF ratio fluorescence sensor, which comprises the following steps: screening and preparing a lanthanide metal organic framework Eu / Tb MOF using 1, 3, 5-dicarboxyphenylboronic acid as a ligand; and 2, detecting Cr (VI) by using an Eu / Tb MOF ratio fluorescence sensor. The Cr (VI) enables the fluorescence absorption peak of the Eu / Tb MOF to be quenched at 545nm and the fluorescence absorption peak of the Eu / Tb MOF to be almost unchanged at 618nm, so that the Eu / Tb MOF ratio fluorescence sensor is constructed to detect the Cr (VI), and the concentration of the Cr (VI) in a to-be-detected sample is judged through fluorescence intensity, color change and CIE chromaticity diagram degree. The Eu / Tb MOF ratio fluorescence sensor can generate more color intervals, Cr (VI with different concentrations can be detected more accurately, and the sensitivity is improved. The method disclosed by the invention has the characteristics of low cost, high response speed, low detection limit and high sensitivity and selectivity.

A pig for use in a pipeline is provided for determining the material of the pipeline in the context of an inline inspection. The pig includes a position determination unit and at least one braking arrangement for immobilizing the pig at a certain position in the pipeline. The pig also includes an X-ray fluorescence sensor.

The invention relates to a fluorine-containing polymer of a specific mononitroalkylbenzene aromatic hydrocarbon explosive tracer as well as a preparation method and application of the fluorine-containing polymer, and belongs to the technical field of fluorescent probes. The method specifically comprises the step of synthesizing a polymer with fluorine atoms as a solid fluorescence sensor probe. The probe realizes the detection of explosives through the interaction between fluorine atoms and nitryl and by doping CdSe / ZnS quantum dots. Based on the fluorine-containing polymer, aromatic alkyl hydrocarbon explosives (tracers) containing a single nitro group can be specifically recognized. Quantum dots are blended with the fluorine polymer, the quantum dots as a nano material are mixed and dispersed with the polymer to form a nanofiber membrane, and the fluorescence of the fluorine-containing quantum dot polymer is changed by further utilizing the interaction between fluorine and silicon atoms and nitro aromatic hydrocarbon, so that the fluorescence of the fluorine-containing quantum dot polymer is changed. Therefore, the specific detection-fluorescence rise of the mononitroalkane explosive is realized (fluorescence decline caused by interference such as light blocking is avoided).

The invention discloses a fluorescence sensor for detecting thromboregulatory protein as well as a preparation method and a use method of the fluorescence sensor. The preparation method comprises the following steps: S1, adding a capture antibody of the thromboregulatory protein into an elisa plate; s2, adding the prepared bovine serum albumin; s3, adding prepared thrombus regulatory protein solutions with different concentrations; s4, adding a prepared detection antibody modified with alkaline phosphatase and used for detecting thromboregulatory protein; s5, adding a p-nitrophenyl disodium phosphate solution, and fully reacting; s6, a NO2-Eu-MOF nanosheet solution is prepared; s7, preparing a 1, 4-benzene diphosphonic acid solution; s8, adding a 1, 4-benzene diphosphonic acid solution into the NO2-Eu-MOF nanosheet solution, and detecting a fluorescence signal; and S9, adding the solution obtained in the step S8 into the solution obtained in the step S5, detecting the fluorescence intensity, and constructing a fluorescence intensity-concentration working curve. According to the invention, the concentration of thromboregulatory protein in the sample can be quantitatively analyzed.

The invention relates to the technical field of sensor preparation and antibiotic detection, in particular to a preparation method of a dual-emission fluorescence sensor, a product and application of the dual-emission fluorescence sensor. The preparation method of the dual-emission fluorescence sensor comprises the following steps: dissolving 1, 3, 5-benzene tricarboxylic acid and melamine in an organic solvent to prepare a suspension; the suspension liquid is subjected to a first heating reaction and then mixed with zinc salt to be subjected to a second heating reaction, and Zn-MBTC is obtained; the preparation method comprises the following steps: dissolving Zn-MBTC and Ru (bpy) 3 < 2 + > in water, carrying out ultrasonic treatment and oscillation, and then washing, centrifuging and drying to obtain the dual-emission fluorescence sensor. The dual-emission fluorescence sensor prepared by the method has the advantages of high sensitivity and high detection speed when being used for detecting four tetracycline antibiotics, namely doxycycline, tetracycline, oxytetracycline and gold tetracycline, and the detection limit is as low as 0.013 mu mol / L.

The invention discloses application of a ratiometric fluorescence sensor based on a fluorescein isothiocyanate labeled metal organic framework in escherichia coli detection. The method comprises the following steps: synthesizing ZTMs by taking TPPS as a ligand, and then fully incubating with FITC to prepare ZTMs (at) FITC; according to the present invention, Escherichia coli is used to reduce Cu < 2 + > to Cu < + >, such that the fluorescence intensity of ZTMs (at) FITC is changed, the fluorescence intensity of the emission peak at the 515 nm position is reduced, the fluorescence intensity of the emission peak at the 683 nm position is increased, and the color of the solution is observed to gradually change from yellow to orange and finally to red under the 365 nm ultraviolet light; and rapid and sensitive detection of the escherichia coli is realized through the ratio of the fluorescence intensities of the two emission peaks. According to the detection method, the accuracy of a detection result is improved, errors caused by environment, instruments and manual operation can be avoided, professional operators are not needed, the method has the advantages of being rapid, simple and independent of expensive instruments and equipment, and the detection cost is reduced.

The present invention discloses a fluorescent probe and a fluorescent label for real-time non-destructive visualization monitoring of the freshness of meat food, and their preparation methods and applications. The structural formula of the fluorescent probe HBT-Bz is simple and easy to synthesize. Using the HBT fluorophore with ESIPT properties and AIE effect as the parent body and benzoxazole ester group as the recognition group for amine compounds, a fluorescence turn-on probe is designed and synthesized. The fluorescent probe HBT-Bz responds quickly and sensitively to amine compounds, with a detection limit as low as 5.1 ppm, and can be used to fabricate a solid fluorescence sensor for detecting volatile amine compounds. The synthetic route of the fluorescent probe involved in the present invention is simple, can recognize a variety of volatile amines, has a low detection limit, and the preparation process of the fluorescent label loaded with the fluorescent probe is simple. When in use, no pretreatment of the food is required. Placing the fluorescent label in the meat food packaging can realize real-time non-destructive visualization monitoring of the food freshness, and the results are accurate and reliable.

The invention discloses a fluorescence sensor protein design method, a product thereof and a detection method, and belongs to the technical field of biology. The design method of the fluorescent sensor protein is characterized in that the distance between amino acids at two ends of an allosteric domain of the protein is less than 10, and the distance change is more than 2. Based on the design method, the invention also provides a fluorescent sensor protein, a gene thereof, a recombinant expression vector, a transformant, a fluorescent sensor and a method for high-throughput real-time detection of intracellular metabolites, and the fluorescent sensor protein comprises allosteric sterol O-acyltransferase and circulating fluorescent protein cpEGFP; the allosteric sterol O-acyltransferase has an amino acid sequence as shown in SEQ ID NO. 1; the circulating fluorescent protein cpEGFP has an amino acid sequence as shown in SEQ ID NO. 2. The fluorescence sensor protein is applied to concentration detection of intracellular sterol substances, detection is more real-time, and a more accurate tool is provided for analysis of related metabolic flow.

The invention designs a preparation method of a magnetic molecular imprinting-aptamer sandwich fluorescence sensor for detecting kanamycin. And the magnetic molecular imprinting composite material Fe3O4 (at) UiO-66 (at) MIP with a specific adsorption target object KANA is prepared. When a detection target object KANA exists, the Fe3O4-coated UiO-66-coated MIP can rapidly and specifically capture the KANA in a short time. Then, a DNA single-stranded aptamer marked with FAM is added, after the aptamer and KANA captured on the surface of the Fe3O4 at-UiO-66 at-MIP are subjected to specific recognition and magnetic separation again, an aptamer probe can enter sediment along with the magnetic composite material Fe3O4 at-UiO-66 at-MIP, the fluorescence intensity of supernate is reduced, and quantitative analysis is conducted according to the fluorescence difference value delta F. When the KANA does not exist, the aptamer cannot enter the precipitate, so that the fluorescence signal intensity is not changed. Double recognition is formed through specific binding of an MIP imprinting cavity and the aptamer, matrix interference is reduced by means of fluorescence characteristics and magnetic separation capacity, the method has the advantages of being high in adsorption capacity, specific in recognition, high in sensitivity, wide in detection range, good in selectivity and the like, and rapid and accurate detection of KANA in a complex sample can be achieved.

The invention relates to the field of veterinary drug residue detection, in particular to a fluorescence sensor based on DNA molecular machine mediated split Cas12a and application of the fluorescence sensor to ofloxacin detection, the fluorescence sensor comprises a system A, a system B and a system C. According to the final concentration of each system, the system A comprises an AP / CP double-stranded compound and a 20 mM Tris-HCl buffer solution 1, the system B comprises a double-stranded compound, an sRNA probe and a 10 mM Tris-HCl buffer solution 2, and the system C comprises a DNA molecular machine mediated split Cas12a. The system C comprises a Cas12a / hRNA compound, a pDNA / psG43 signal probe, ThT and a 20 mM Tris-HCl buffer solution; aP in the AP / CP double-chain compound is an aptamer of ofloxacin, and AP and CP in the AP / CP double-chain compound are paired and connected through complementary base pairing; the sequences of AP, CP, PS, SS, sRNA, hRNA, pDNA and psG43 are respectively as shown in SEQ ID NO.1-SEQ ID NO.8. The invention also discloses a kit for detecting the content of the protein. According to the application, the nucleic acid aptamer of ofloxacin is utilized, based on a DNA molecular machine and SCas12a as a signal amplification technology and sulfo-modified G43 as a fluorescent signal probe, even if the content of ofloxacin in a sample is extremely low, detection can be accurately realized.

The invention discloses nitrogen-doped carbon quantum dots derived from American cockroach dregs as well as a preparation method and application of the nitrogen-doped carbon quantum dots, and belongs to the technical field of nitrogen-doped carbon quantum dots. The nitrogen-doped carbon quantum dots are prepared from the American cockroach dregs by a one-step hydrothermal method, optimal synthesis parameters are obtained through experimental optimization of synthesis conditions, and the prepared nitrogen-doped carbon quantum dots have excellent optical characteristics and stability; the invention also provides an application of the nitrogen-doped carbon quantum dot in Ag < + > detection, the nitrogen-doped carbon quantum dot has good selectivity, sensitivity and anti-interference performance on Ag < + >, a detection mechanism is an internal filtering effect and light-induced electron transfer, and the nitrogen-doped carbon quantum dot can be used as a preferred fluorescent material of an Ag < + > fluorescent sensor. The nitrogen-doped carbon quantum dots synthesized by the method not only have an Ag < + > sensing function, but also realize high-valued utilization of medicine residues, and also provide a new thought for self-doping of heteroatoms.

The invention provides a fluorescence sensor 4-MU (at) ZIF-8 (at) PLA-PEG as well as a preparation method and application thereof, and belongs to the technical field of visual food safety monitoring materials. The fluorescence sensor comprises a zeolite imidazate framework ZIF-8, an organic dye molecule 4-MU and a polymeric macromolecule PLA-PEG (Polylactic Acid-Polyethylene Glycol), the organic dye molecule 4-MU is coated with the zeolite imidazate framework ZIF-8 to form 4-MU (ZIF-8 nano particles with a core-shell structure, and the 4-MU (ZIF-8 nano particles with the core-shell structure are doped into a polymeric macromolecule PLA-PEG (Polylactic Acid-Polyethylene Glycol). The ZIF-8, 4-MU and PLA-PEG in the fluorescence sensor provided by the invention can cooperate with each other, can visually detect TCs with high response speed and low detection limit, and has certain mechanical properties and antibacterial properties, thereby providing a new thought for the field of intelligent packaging for detecting TCs in food at present.

The invention relates to the technical field of biomedical detection, and discloses a portable urine component detection system and method based on a fluorescence sensor array. The system comprises a fluorescence sensor array, different fluorescence probes are used for detecting different trace components in urine, and fluorescence color gradient changes are generated through a probe-target object reaction, so that bilirubin, glucose and albumin in the urine are detected. The detection system comprises a micro-fluidic chip which integrates a capillary driving channel and a colorimetric reaction area and is used for sample transmission and reaction area positioning; an ultraviolet excitation light source and a multi-angle optical sensor are arranged in the camera obscura type color acquisition module, and the camera obscura type color acquisition module eliminates ambient light interference and captures fluorescence chromaticity signals; and the intelligent data processing system is integrated with an intelligent analysis end of the smart phone, establishes a quantitative relationship between chromaticity parameters (RGB and hue saturation) and the concentration of a target object through a machine learning model, and outputs a detection result in combination with an ambient light correction algorithm.

The invention discloses a radio frequency thermal therapy equipment testing system, relates to the technical field of equipment testing, and is used for solving the problems that the uncertainty in the energy transmission process is increased, and the system output stability and response precision are reduced. The output power is calculated in combination with an analog multiplier, and difference analysis is carried out in cooperation with ideal power; meanwhile, performing fast Fourier transform on the original signal to extract a frequency concentration ratio, and calculating a heat concentration coefficient; the tissue temperature at a needle is monitored based on an optical fiber fluorescence sensor, fluorescence detection temperature and observation temperature values are calculated in combination with fluorescence lifetime, difference analysis is performed to obtain a thermal response deviation factor, and the two values are fused to obtain a feedback calibration result for equipment performance optimization, and real-time monitoring of an energy state and abnormal heat accumulation identification are realized.

The invention discloses a hemicyanine fluorescent dye-based compound as well as a preparation method and application thereof. The invention provides a compound MCYA based on hemicyanine fluorescent dye targeting AChE, the compound has high sensitivity to AChE, the detection limit can be as low as 0.0225 U / mL, and high-sensitivity detection on AChE can be realized; and the MCYA has good selectivity and strong anti-interference capability on common ions and amino acids in a biological system. A fluorescence sensor platform built by combining MCYA and AChE can realize qualitative and quantitative analysis of organophosphorus and carbamate pesticide residues, and has the advantages of high sensitivity, simplicity in operation, low cost, short detection time, suitability for rapid detection in the market and the like.

The application provides a fluorescent sensor based on a tripod [5] arene, which is obtained by self-assembly complexation of a host of a tripod [5] arene TP5 and a guest of a quaternary ammonium salt in an H2O-DMSO system. A supramolecular system is constructed by host-guest interaction between the tripod [5] arene TP5 and the amphiphilic guest TPN, and then PbS fluorescent nanoparticles are generated in situ under ion induction. Fluorescence enhancement and high-sensitivity detection (LOD=7.00*10 2+ -6 M) of Pb ‑8 are realized. In addition, the fluorescent supramolecular polymer network based on the PbS fluorescent nanoparticles can be successfully applied to in-situ detection of Pb 2+ in cells.

The invention discloses a preparation method of a portable paper-based ratiometric fluorescence sensor APBA-CsPbBr3 at-UiO-66 / Ru, which comprises the following steps: 1, adding lead bromide and cesium bromide into N, N-dimethylformamide, then adding 5-bromovaleric acid and an oleylamine ligand, and stirring; 2, perfluorooctyltriethoxysilane is added, and a precursor solution is obtained; 3, adding zirconium chloride and terephthalic acid into N, N-dimethylformamide to obtain a homogeneous solution; 4, after heating, UiO-66 is obtained; 5, stirring the precursor solution, APBA, UiO-66 and tris (2, 2-dipyridyl) ruthenium chloride hexahydrate to obtain a composite material; and 6, dropwise adding the composite material solution into the filter paper. The APBA modified material is adopted as a recognition unit, the APBA modified material and the paper base construct a fluorescence sensing platform, visual quantitative analysis of oxytetracycline in water is achieved, and the device is convenient to carry, low in detection limit, high in anti-interference performance, easy and convenient to operate and suitable for on-site rapid detection of oxytetracycline.

The invention belongs to the field of fluorescent sensors, and relates to an Ag-CP fluorescent probe material as well as a preparation method and application thereof. The structural formula of the fluorescent probe material is [Ag (HL) (L)] n, and an HL ligand is 4-(3, 5-dimethyl-1H-pyrazole-1-yl) benzoic acid. Ag-CP is prepared by the following method: dissolving HL and AgNO3 in H2O to obtain light brown turbid liquid, carrying out high-temperature reaction on the light brown turbid liquid, and cooling to obtain the Ag-CP. The Ag-CP material can identify tryptophan (Trp) molecules in an aqueous solution with high selectivity and high sensitivity, has good stability and anti-interference ability in fluorescence identification ability, and can be used as an excellent Trp molecule identification fluorescence sensor.