The invention belongs to the field of supermolecular compound, and discloses a supermolecular compound, a preparation method thereof, and a method used for distinguishing microorganisms. The supermolecular compound possesses an inclusion complex structure composed of a conjugated
polymer with a structure represented by formula (I), and
cucurbituril [7], wherein R1 is used for representing one of structures represented by formula (II), formula (III), or formula (IV), n is used for representing an integer
ranging from 30 to 300, R2, R3, and R4 are independently used for representing C1-C5
alkyl, Y is used for representing an alkylene with 6 to 12 carbon atoms, or one group with a general structure of -R5-O-R6- or -OR7-, R5, R6, and R7 are used for representing alkylenes, the
carbon atom number of R7 ranges from 6 to 12, and X is used for representing a
halogen. The method is high in speed, efficiency, and accuracy, and is convenient in distinguishing microorganisms such as
bacteria,
fungus, and
virus.