161results about How to "Achieve synthesis" patented technology

The invention provides a method for improving synthesis of nucleic acid under a non-diagnostic constant-temperature condition, a kit and application, and relates to the technical field of bioengineering. Provided are: a nucleic acid having an F2 region capable of annealing with F2c in an F region on the same chain at the 3'end, and an R2c region capable of annealing with R2 in an R region on the same chain at the 5 'end; taking the nucleic acid as a template, taking the 3'end of the F1c region annealed with the F1 region as a synthesis starting point, and synthesizing a complementary nucleic acid chain of the nucleic acid; and performing complementary chain synthesis through a polymerase catalytic chain displacement type synthesis reaction so as to replace the complementary nucleic acid chain synthesized in the previous step. The method has the main advantage that a new combination of short-chain primers can be obtained through simple transformation to trigger rapid amplification of gene fragments by applying a single-enzyme constant-temperature system to short-chain nucleic acid fragments.

The invention relates to an application of oxygenase in catalyzing 4-ethene guaiacol to synthesize vanillin. The amino acid sequence of the oxygenase comprises a sequence as shown in SEQ ID NO.1. The invention also relates to an application of the oxygenase in catalyzing 4-ethene guaiacol to synthesize vanillin. The invention provides an application of oxygenase in catalyzing synthesis of vanillin from 4-ethene guaiacol. The 4-ethene guaiacol oxygenase is in soluble expression in host bacteria. Crude enzyme liquid expressed by the strain stably catalyzes conversion of 4-ethylene guaiacol into vanillin, the conversion rate within 2 hours is 80%, the conversion rate within 24 hours exceeds 98%, and 11.5 g / L of vanillin can be accumulatively produced within 6 hours.

The invention relates to the technical field of energy materials, and discloses a self-assembled low-crystallization nanoflower-shaped polyoxometallate catalyst and a preparation method and application thereof.The preparation method comprises the steps that firstly, cobalt nitrate hexahydrate, iron nitrate nonahydrate, CH4N2O and Finke Co are used as raw materials, Finke Co and Fe < 3 + > are grown on nickel foam in situ, then the Finke Co and Fe < 3 + > react with ZIF-67 (Co), and a Finke Co-FeCoNi precursor with a stable structure is prepared; finally, the precursor is subjected to vulcanization, and the nano flower-shaped polyoxometallate catalyst Finke Co-FeCoNi / S is obtained. According to the preparation method, Finke Co is added, so that the crystallinity of the catalyst material is reduced, the catalyst material is assembled into a nanoflower-shaped structure, the low crystallinity can provide more coordinated unsaturated sites and improve the conductivity, and the nanoflower-shaped form exposes more defect sites, so that the reaction contact area is increased, and the catalytic activity is improved.

The embodiment of the invention discloses a three-dimensional reconstruction method. The method comprises the following steps: acquiring a satellite image containing a target area; obtaining a target area image and a camera model parameter corresponding to the target area image by using the satellite image; constructing a point cloud by using the target area image and camera model parameters corresponding to the target area image; performing three-dimensional Gaussian splashing processing on the point cloud to obtain a three-dimensional real scene model of the target area; wherein the original point of a camera coordinate system used by the three-dimensional Gaussian splash processing is determined according to camera model parameters. The satellite image is used as an image input source for three-dimensional reconstruction, a whole three-dimensional reconstruction process suitable for the satellite image is constructed based on the three-dimensional Gaussian splashing technology, and three-dimensional reconstruction with the satellite image as the input source is achieved.

The invention relates to an RP phase perovskite nanocrystal modified three-dimensional perovskite solar cell. The basic structure of the solar cell device comprises a transparent conductive substrate, an electron transport layer, a three-dimensional perovskite light absorption layer, an RP phase perovskite nanocrystalline layer, a hole transport layer and an electrode. The structural formula of the RP-phase perovskite nanocrystal is Cs2PbX4, wherein X is one or more of halide ions I <->, Br <-> and Cl <->; and the thickness of the film is 0.1-100nm. According to the method, the RP-phase perovskite nanocrystals can be synthesized in the air, the preparation process is simple, the synthesis cost is low, the quality of the RP-phase nanocrystal modified three-dimensional perovskite thin film is improved, the defect state density is reduced, the carrier recombination is reduced, and the photoelectric conversion efficiency of a solar cell device is remarkably improved.

The invention provides a polarization and spectrum combined laser synthesis system and method, and belongs to the field of laser beam synthesis, and the system comprises a laser seed source, an optical fiber beam splitting module, a phase control module, an optical fiber output module, a two-dimensional polarization coherent beam combination module and a dispersion element which are connected in sequence; the two-dimensional polarization coherent beam combination module is of a spatial three-dimensional structure; the number of the laser seed sources is m, the laser seed sources are arranged in parallel, each laser seed source corresponds to one optical fiber beam splitting module, and laser emitted by each laser seed source is divided into 2n sub-beams which have the same wavelength and are parallel to each other through the optical fiber beam splitting module, the 2n sub light beams respectively correspond to 2n phase control modules, 2n optical fiber output modules and the same two-dimensional polarization coherent beam combining module; the 2n sub-light beams are coherently combined into the same laser beam, and the m laser spectrums with different wavelengths are combined to obtain the high-power laser. According to the invention, high-power laser is synthesized by using the integrated light beam synthesis system.

The invention provides a preparation method of jujube juice rich in fructan, the jujube juice rich in fructan and application of the jujube juice rich in fructan, and particularly belongs to the technical field of fruit juice preparation. The preparation method of the fructan-rich jujube juice comprises the following steps: mixing immobilized fructan sucrase with jujube juice, carrying out sugar conversion, centrifuging, and discarding precipitate, so as to obtain the fructan-rich jujube juice, the nucleotide sequence of the gene for coding the fructan sucrase is as shown in SEQ ID NO. 1. According to the preparation method disclosed by the invention, the sucrose in the jujube juice can be efficiently converted into the fructan, and the sucrose is converted into the fructan while the content of the easily utilized sugar (sucrose) is reduced.

The embodiment of the invention provides a video-audio generation method and system based on semantic information guidance. The method comprises the following steps: inputting a video frame and label information of a video, which is used for detecting sound object appearance guidance, into a pre-training model of a contrast learning language-image to obtain a video hidden layer representation of the video frame and a text hidden layer representation of the label information; determining the semantic similarity of the text hidden layer representation and the video hidden layer representation; taking the semantic similarity as guidance to predict a timestamp of a sounding node of the video; and linearly projecting the video hidden layer representation into the audio synthesis model, and controlling the audio synthesis model to generate an audio aligned with the video according to the timestamp by using a spatio-temporal information regulator. According to the embodiment of the invention, whether the audio needs to be generated at each moment of the video is determined according to the video frame, the label information and the similarity between the video information and the data label information, so that the audio with better video-audio granularity alignment is generated by introducing the guidance of the data label information.

The invention discloses an automatic driving scene adaptive single object three-dimensional reconstruction and image point cloud synthesis method, and relates to the technical field of automatic driving scene data production. The method comprises the following steps: respectively projecting each vertex of a box of a target object in a three-dimensional space to each frame, then carrying out image segmentation, setting the opaqueness of a part except the segmented object to be 0, taking the part as training input, and supervising the object by using the opaqueness; rendering is carried out in combination with the parameters of Scaffold-GS and the adaptive parameters of the camera to obtain a rendered image, and training loss is calculated to update the Gaussian point cloud; the camera poses of the first frames of the training scene and the target scene are aligned to determine the placement position of the target object, then the target object is rendered to obtain the multi-view object image, and the multi-view object image can be directly pasted with the target scene image. According to the method, the single object three-dimensional representation without edge blur is generated, and the object is rendered in the target scene.

The invention relates to a preparation method and application of a wall coating type enzyme-loaded microchannel reactor. The method comprises the following steps: mixing tetramethyl orthosilicate, methyltrimethoxysilane and methanol to obtain a solution A; deionized water, polyethylene glycol, a sodium fluoride aqueous solution and a dialyzed lipase solution are mixed to obtain a solution B, the solution B and the solution B are mixed for 3-10 minutes in an ice bath environment to obtain a solution C, the solution C is introduced into the pretreated microchannel through an injection pump, after incubation is conducted for 10-60 minutes, nitrogen is adopted for purging, redundant sol-gel is removed, and then the solution C is put into a drying oven to be dried; the catalyst is used for hydrolysis reaction of grease and various ester exchange and esterification reactions. The method can be carried out in a mild environment, and has higher conversion rate and shorter reaction time.

The invention discloses a preparation method of a beta-aryl beta-pyridyl alkyl ketone compound, which comprises the following steps: in the presence of a photocatalyst and under the condition of blue light irradiation, reacting a compound shown as a formula (1), a compound shown as a formula (2) and a compound shown as a formula (3) to generate the beta-aryl beta-pyridyl alkyl ketone compound shown as a formula (4), wherein Ar is selected from substituted or unsubstituted aryl, naphthyl and heteroaryl; the compound in the formula (2) is 4-cyanopyridine; the compound of formula (3) is aldehyde; and the photocatalyst is selected from tetrabutyl ammonium decatungstate (TBADT), 9-thioxanthone and a benzophenone type photocatalyst. The preparation method provided by the invention has the advantages of cheap and easily available reaction raw materials, simple reaction operation, wide application range of reaction substrates, easy amplification of reaction, mild reaction conditions and the like, and can realize industrial production and application. # imgabs0 #

The present invention relates to a conductive separator plate with a gas-liquid distribution flow field structure and a processing method thereof, wherein functional partitions such as a sealant wire trough, a guide trough, a gas channel, and a liquid channel are integrated into the same component and formed in one piece. Compared with traditional conductive separator plates, the conductive separator plate of the present invention reduces the number of processing times and the difficulty of processing, so that the manufacturing of the conductive separator plate, the assembly quality of the battery stack, and the positioning accuracy are all guaranteed. The conductive separator plate of the present invention does not require separate reinforcement of the sealant wire trough, and can ensure the operating stability of the electrolytic cell under the condition of producing high-pressure hydrogen (>3.5MPa). Compared with the prior art, the conductive separator plate of the present invention has a simple structure, is easy and reliable to install, reduces the processing steps, and improves the structural reliability.

The invention discloses a preparation method of an ultralow-refractive-index and high-transparency cycloolefin copolymer, and belongs to the technical field of polymer preparation, the method comprises the following steps: in the presence of a Grubbs catalyst and an organic solvent, carrying out ring-opening metathesis polymerization reaction on a cycloolefin monomer and a fluorine-containing cycloolefin monomer to obtain the ultralow-refractive-index and high-transparency cycloolefin copolymer. The unsaturated fluorine-containing cycloolefin copolymer is prepared; and carrying out hydrogenation reaction on the unsaturated fluorine-containing cycloolefin copolymer and a hydrogen source to obtain the fluorine-containing cycloolefin copolymer. The fluorine-containing cycloolefin copolymer prepared by the method disclosed by the invention has the excellent properties of ultralow refractive index, high transparency, high thermal stability, excellent mechanical property, amorphous form and the like, and the refractive index is adjustable in a range of 1.37-1.47; the ultralow refractive index is far lower than that of the conventional typical organic low-refractive-index commercial material PMMA (the refractive index is 1.49). Therefore, the fluorine-containing cycloolefin copolymer prepared by the method can be widely applied to the optical field, such as low-refractive-index optical lenses (AR and VR glasses), anti-reflection coatings and the like.

The embodiment of the invention provides a maritime unknown scene image synthesis method and device based on a diffusion model. The method comprises the following steps: adjusting the posture of a foreground target, and generating target images with different postures by inputting a single foreground target image; generating background images under different environment and weather conditions by inputting a single background image and semantic information; and performing image fusion by using a neural network by calculating and adjusting the size and proportion of the foreground image in the background image. By means of the target and image synthesis method, image synthesis of a new scene is achieved, simulation of an unknown scene can be rapidly achieved, the unknown scene serves as a training and verification data set of intelligent algorithms such as detection and recognition of a marine target and environment, the problem that marine task scene data is deficient can be solved with low cost and high efficiency, and the target and image synthesis efficiency is improved. A cultivation environment is provided for intelligent training and ability verification of the offshore unmanned system, and iterative upgrading of the intelligent level of the offshore unmanned system is accelerated.

The invention relates to a preparation and synthesis method of an amino-substituted nitrogen-containing heterocyclic ring, which is carried out by using an ice bath auxiliary device, and is characterized by comprising the following steps: adding p-aminobenzaldehyde into a toluene solution of aminoacetaldehyde dimethyl acetal, heating to reflux to obtain (Z)-N-(4-acetamido-benzylidene)-2, 2-dimethoxyethylamine, and reacting for 2-4 hours to obtain the N-(4-acetamido-benzylidene)-2, 2-dimethoxyethylamine. And carrying out three steps to obtain the 6-aminoisoquinoline. Wherein the reaction liquid is poured into the crushed ice, an ice bath auxiliary device is used for implementing, and the ice bath auxiliary device comprises an ice crusher (1), a carrying table (2), a heating plate array (3) and a tray (4).

The invention discloses a recombinant saccharomyces cerevisiae engineering bacterium for producing 7-dehydrocholesterol and application, and belongs to the technical field of genetic engineering and biological engineering. On the basis of an original strain, an exogenous gene DHCR24 and key endogenous genes ERG2 and ERG3 are integrated, and a non-path gene ROX1 is knocked out, so that the accumulation of 7-dehydrocholesterol in a saccharomyces cerevisiae body is further improved; the accumulation amount of the 7-dehydrocholesterol is further increased by adding integration of a speed limiting step gene and cell growth promoting genes SET2 and HAP4 and knockout of an RPH1 gene, and the ability of synthesizing the 7-dehydrocholesterol from the beginning in a saccharomyces cerevisiae body is further improved by completing single-copy integration of TPS1, ERG25, ERG26 and ERG27 genes at a saccharomyces cerevisiae GAL80 site. And the yield of 7-dehydrocholesterol reaches 896.2 mg / L after the constructed strain is subjected to shake flask fermentation for 100 hours.

The invention discloses a preparation method of a 4-(2, 2-dimethyl-2-cyanoethyl)-beta-lactam derivative, which comprises the following steps: by taking a substituted N-(5-iodoquinoline-8-yl)-3-butene amide derivative as a substrate and azodiisobutyronitrile as a free radical source, reacting in an organic solvent at 80-110 DEG C under the action of a copper salt catalyst to obtain the 4-(2, 2-dimethyl-2-cyanoethyl)-beta-lactam derivative. The invention relates to a 2, 2-dimethyl-2-cyanoethyl)-beta-lactam derivative. The preparation method provided by the invention has the advantages of easily available raw materials, mild reaction conditions, simple reaction operation and post-treatment process, high yield, good universality, greenness and environmental protection, and is suitable for large-scale production.

The invention discloses a method for biosynthesizing dicarbon organic acid by using CO2, and belongs to the technical field of bioengineering. A path for synthesizing glyoxylic acid and glycollic acid by taking CO2 as a substrate is designed, and a first reaction module for converting CO2 and acetyl coenzyme A into pyruvic acid and a second reaction module for converting pyruvic acid into malic acid are obtained through thermodynamic evaluation and screening of pathase. The third reaction module is used for converting malic acid into acetyl coenzyme A and glyoxylic acid, the fourth reaction module is used for converting glyoxylic acid into glycollic acid, and PSP circulation is carried out on the four reaction modules. Through an experimental method of a one-pot two-step method, 2.78 g / L of glyoxylic acid can be realized after 11 hours of catalytic reaction in total; and 1.78 g / L of glycollic acid. The invention successfully realizes a cell-free biosynthesis method for synthesizing glyoxylic acid and glycollic acid by taking CO2 as a substrate.

The invention relates to a preparation method of TiO2 and TiO2 / ZnO heterojunction based on a hollow structure and application of the TiO2 and TiO2 / ZnO heterojunction in an ultraviolet detector, the hollow structure TiO2 is a TiO2 hollow sphere, a TiO2 hollow cube and a TiO2 hollow dodecahedron, the hollow heterostructure is composed of the hollow structure TiO2 and a ZnO nanorod, the ZnO nanorod grows on the surface of the TiO2 hollow structure, and the whole ZnO nanorod is hedgehog-shaped; the prepared hollow-structure TiO2 and TiO2 / ZnO heterojunction is coated on a conductive surface of FTO conductive glass to prepare a working electrode, the working electrode is connected with a counter electrode through a heat sealing film, electrolyte is injected into an internal cavity to obtain the ultraviolet detector with high detection performance, and the ultraviolet detector is suitable for the field of ultraviolet detection. The method adopted by the invention has the advantages of simplicity in operation, low cost and high universality, different hollow-structure TiO2 and hedgehog-shaped TiO2 / ZnO heterojunctions such as spherical, cubic and dodecahedron heterojunctions are synthesized, an ultraviolet detector with a self-energy-supply characteristic is further prepared, and the application of a TiO2 nano material in the field of photoelectric detection is expanded.

The invention belongs to the technical field of material science and electro-catalysis, and discloses an amine-modified copper gas diffusion electrode and pulse electro-catalysis C-N coupling application thereof.The amine-modified copper gas diffusion electrode is obtained by spraying and loading copper nanoparticles on the surface of carbon paper to obtain the copper gas diffusion electrode, and then continuously spraying and loading amine on the surface of the copper gas diffusion electrode to obtain the amine-modified copper gas diffusion electrode. The method is easy to operate, the obtained gas diffusion electrode is stable in structure and uniform and effective in amine modification, when the gas diffusion electrode is used for electro-catalysis C-N coupling, the microenvironment on the surface of a catalyst can be regulated and controlled through pulse potential, C-N coupling reaction is promoted, and high urea yield and Faraday efficiency are achieved.

The invention discloses a composition with an anti-aging effect and a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition is prepared by compounding a traditional Chinese medicine active component, a moisturizing and permeation promoting component and a repairing and anti-aging auxiliary component, and the traditional Chinese medicine active component is composed of three zymolytes and three fermentation extracts which are respectively prepared from six medicinal and edible traditional Chinese medicine raw materials through differentiated composite enzymolysis and probiotic anaerobic fermentation processes. The problems that traditional Chinese medicine extracts are low in transdermal absorption rate, poor in bioavailability and insufficient in cosmetic system stability are solved, active ingredients are small-molecularized through process optimization, a multi-target synergistic anti-aging system is formed by matching with functional components, the anti-aging and repairing composition has excellent anti-aging and repairing effects, physical and chemical stability and skin safety, and the anti-aging and repairing composition has a good application prospect. The composition is suitable for various skin types and can be widely applied to preparation of anti-aging repair cosmetics.

The invention belongs to the field of fluorine chemical industry, and particularly relates to a microchannel reaction tube bundle and a device and method for continuously preparing hexafluoropropylene oxide. The micro-channel reaction tube bundle comprises one or more parallel micro-channel reactors; each micro-channel reactor comprises a plurality of continuous reaction units; and each reaction unit comprises an S-shaped ascending section and a vertical descending section. When the microchannel reaction device provided by the invention is used for preparing hexafluoropropylene oxide, continuous synthesis can be realized under the conditions of normal temperature and lower pressure. The reaction is carried out in the micro-channel reactor, an amplification experiment is carried out, and the productivity reaches a pilot scale.

The present invention provides a method for preparing an isothiazole-5(2H)-imine compound and its application. The method uses a thioacrylamide compound shown in formula I as a raw material, and a polysubstituted isothiazole-5(2H)-imine compound shown in formula II undergoes a ring-forming reaction under the action of an organic high-valent iodine compound. Compared with the preparation method of polysubstituted isothiazole-5(2H)-imine compounds in the prior art, the present invention utilizes an organic high-valent iodine compound to participate in the ring-forming reaction of the thioacrylamide compound, thereby realizing the preparation of polysubstituted isothiazole-5(2H)-imine compounds. The synthesis method has the advantages of easy availability of raw materials, mild reaction conditions, no safety risks, and no need to use a metal catalyst.

The invention relates to a gas hydrate stable preservation device, system and method, and the device comprises an upper housing which is provided with a first cylinder and a top cover, and the bottom of the first cylinder is open and is provided with a switch valve; the piston reciprocates in the first barrel, a first pressure cavity is defined between one side of the piston and the first barrel, a second pressure cavity is defined between the other side of the piston and the first barrel, the second pressure cavity contains pressurized liquid, a first channel and a second channel are formed in the piston, and a spraying head is arranged at the bottom of the second channel; the lower shell is provided with a second barrel and a bottom plate located on the second barrel, the top of the second barrel is open and detachably connected with the switch valve, pressurized liquid is injected into the second pressure cavity to drive the piston to move towards the extending position, and the gas hydrate in the first pressure cavity is compressed and formed under pressurization of the piston to obtain the solid gas hydrate. And then the switching valve is opened, and the solid gas hydrate is transferred to the lower shell for low-pressure storage.

The invention relates to the field of synthetic rubber, and discloses an ethylene-butadiene polymer, a preparation method thereof and vulcanized rubber. The ethylene-butadiene polymer comprises a polyethylene chain segment and a butadiene rubber chain segment; based on the total weight of the ethylene-butadiene polymer, the content of the polyethylene chain segment is 0.1-40 wt%, and the content of the butadiene rubber chain segment is 60-99.9 wt%; in the butadiene rubber chain segment, based on the total amount of the butadiene rubber chain segment, the content of a cis-1, 4-structure is 90-98.5 mol%; based on the total weight of the ethylene-butadiene polymer, the content of toluene insoluble substances is 0.3-9 wt%. According to the ethylene-butadiene polymer, an ethylene structure chain segment is introduced into a butadiene structure chain segment of butadiene rubber, so that the butadiene rubber is modified, and the ethylene-butadiene polymer has improved tensile strength and tear resistance.

The present application relates to the technical field of porphyrin-based covalent organic framework material preparation, in particular to a mild preparation method of acetaldehyde-based porphyrin covalent organic framework and application thereof, which comprises the following steps: step one: dispersing ligand 2', 5'-bis((ethyl sulfide) methyl)-[1,1':4',1''-triphenyl]-4,4''-diamine in acetonitrile solution to obtain solution A, and dissolving acetaldehyde-based porphyrin in dichloromethane to obtain solution B; step two: mixing solution A and solution B; step three: adding ferric chloride and acetic acid as catalysts to react under mild conditions to obtain acetaldehyde-based porphyrin covalent organic framework material, which has excellent gaseous iodine adsorption performance. The synthesis method provided by the present application has mild conditions, simplifies the high-temperature time-consuming shortcomings of traditional porphyrin-based covalent organic framework material synthesis, and realizes the synthesis of special ligand covalent organic framework.

The invention relates to the technical field of image processing, and discloses a railway container corner fitting abnormal state detection method and related equipment. The method comprises the steps of obtaining a railway freight no-load vehicle passing image set, and extracting an F-TR lock image in each no-load vehicle passing image based on an image segmentation algorithm; obtaining a railway freight loading box vehicle passing image set, and superposing each F-TR lock image to a preset corner fitting position of each loading box vehicle passing image in the loading box vehicle passing image set to obtain a training data set; performing model training based on the training data set to obtain a target detection model taking an F-TR lock as a detection target; and performing F-TR lock detection on the to-be-identified loading box vehicle passing image based on the target detection model, and determining an abnormal state of the boxing corner fitting according to a detection result. According to the method, the data set can be constructed under the condition of sparse samples, and the prediction quality of a machine learning model is improved, so that the abnormal state of the container corner fitting is effectively identified and alarmed.

The invention discloses a synthesis method of an N-trifluoromethylthio isochromene-1-imine compound, and belongs to the field of organic chemistry. According to the method disclosed by the invention, a 2-alkynyl aromatic amide compound and N-trifluoromethylthio saccharin are taken as raw materials, and the polysubstituted N-trifluoromethylthio isochromene-1-imine compound can be synthesized in one step under the condition of not adding any metal catalyst and organic ligand. The method has the advantages of wide substrate applicability, simple and easily available raw materials and low economic cost; besides, the synthesis of the target product is realized by the method, the target product can be obtained at a relatively high yield only by reacting for 12-14 hours at room temperature, and the target compound has wide application in the fields of medicines, pesticides, functional materials and the like.

The invention relates to the technical field of coupled antennas, and discloses a GNSS coupled antenna comprising an antenna plate which is detachably connected with a reflecting surface. A radio frequency connector is arranged on the reflecting surface; a shielding cover is arranged at the bottom of the reflecting surface; the radio frequency connector and the shielding cover are positioned right above the reflecting surface; radiating units are arranged on the front, back and side surfaces of the antenna plate. According to the GNSS coupled antenna, under the condition that stable performance is ensured, probe excitation is used, signals are connected with the radiation unit in a coupling mode, multi-frequency output is achieved, the requirements for multi-system compatibility and high-precision measurement of existing measurement equipment are met, and meanwhile wireless communication data transmission is met.

This invention discloses a chiral monophosphine ligand, its preparation method, and its application in asymmetric synthesis. The ligand preparation method involves mixing an N,N',1,2-tetrasubstituted ethane-1,2-diamine compound, phosphorus trichloride, triethylamine, and solvent under a nitrogen or argon atmosphere at -80 to -70°C, and reacting at 60 to 80°C for 6 to 8 hours. The resulting reaction mixture is then cooled to room temperature, and an alcohol, phenol, or Grignard reagent is added dropwise, reacting at 60 to 80°C for 8 to 12 hours. The resulting mixture is then post-treated to obtain the chiral monophosphine ligand. The application involves stirring a nickel catalyst, a chiral monophosphine ligand, a solvent, a diene, and an aldehyde under an argon or nitrogen atmosphere at a specified temperature for 12 to 15 hours. The mixture is then post-treated to obtain the target product. This method utilizes widely available and inexpensive raw materials, and the operation is simple, achieving high stereoselectivity and enantioselectivity in the synthesis of alcohol compounds containing chiral conjugated dienes through a one-step reaction.