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247 results about "Acyltransferase" patented technology
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Acyltransferase is a type of transferase enzyme that acts upon acyl groups.
High arachidonic acid producing strains of Yarrowia lipolytica
Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.
Owner:DUPONT US HLDG LLC
Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems
InactiveUS20070245431A1Improve the level ofReduce competitionOther foreign material introduction processesOxidoreductasesBiotechnologyHeterologous
Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
Owner:SEMBIOSYS GENETICS INC +1
Docosahexaenoic acid producing strains of Yarrowia lipolytica
Owner:DUPONT US HLDG LLC
Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
ActiveUS20070270494A1Improve the level ofReduce competitionAntibacterial agentsOrganic active ingredientsHeterologousAcyl-CoA synthetase
Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
Owner:DSM IP ASSETS BV
Diacylglycerol o-acyltransferase
InactiveUS6344548B1Sugar derivativesPeptide/protein ingredientsDiglyceride acyltransferase activityTriacylglycerol VLDL
Nucleic acid compositions encoding polypeptide products with diglyceride acyltransferase activity, as well as the polypeptide products encoded thereby and methods for producing the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications, as well as in treatment therapies and in the production of triacylglycerols.
Owner:VETERANS AFFAIRS DEPT OF +3
Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.
Owner:DUPONT US HLDG LLC
Acetyl-CoA acyltransferase gene disrupted bacterium for producing polyhydroxyalkanoate and method for producing polyhydroxyalkanoate using the same
A method for producing a polyhydroxyalkanoate with improved productivity and composition is provided. Polyhydroxyalkanoate is produced by a bacterium for producing polyhydroxyalkanoate in which a gene encoding acetyl-CoA acyltransferase is disrupted.
Owner:CANON KK
Plant diacylglycerol O-acyltransferase and uses thereof
Plant nucleic acid compositions encoding polypeptide products with diacylglyceride acyltransferase (DGAT) activity, as well as the polypeptide products encoded thereby and methods for producing the same, are provided. Methods and compositions for modulating DGAT activity in a plant, particularly DGAT activity in plant seeds, and transgenic plants with altered DGAT activity are provided. Such plants and seeds are useful in the production of human food and animal feedstuff, and have several other industrial applications. Also provided are methods for making triglycerides and triglyceride compositions, as well as the compositions produced by these methods. The subject methods and compositions find use in a variety of different applications, including research, medicine, agriculture and industry.
Owner:RGT UNIV OF CALIFORNIA
Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.
Owner:EI DU PONT DE NEMOURS & CO
Method
InactiveUS20080063783A1Accumulation is reduced and eliminatedIncreased amount of free fatty acidMilk preparationDough treatmentMonoglycerideGlycerol
A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
Owner:DUPONT NUTRITION BIOSCIENCES APS
Method
The present invention relates to a method of reducing and / or removing diglyceride from an edible oil, comprising a) admixing an edible oil with an acyl acceptor substrate and a diglyceride:glycerol acyltransferase, wherein the diglyceride:glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. Preferably, the diglyceride:glycerol acyltransferase comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S. Furthermore the present invention relates to the use of a diglyceride:glycerol acyltransferase characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol, in the manufacture of an edible oil, for reducing and / or removing (preferably selectively reducing and / or removing) diglyceride from said edible oil and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.
Owner:DUPONT NUTRITION BIOSCIENCES APS
Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
Two acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, the genes encoding phophatidylcholine-diacylglycerol acyltransferase (PDAT) and diacylglycerol acyltransferase (DGAT2) have been isolated from Y. lipolytica. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
Owner:EI DU PONT DE NEMOURS & CO
Method
InactiveUS20060078648A1Accumulation is reduced and eliminatedIncreased amount of free fatty acidFatty acid esterificationHydrolasesMonoglycerideGlycerol
A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an addition emulsifier.
Owner:DUPONT NUTRITION BIOSCIENCES APS
Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
InactiveCN101573451AImprove the level ofReduce competitionMicrobiological testing/measurementAcyl Coenzyme A SynthetasesBiotechnology
Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or toincrease the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils o btained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
Owner:DSM IP ASSETS BV
Acyltransferase
InactiveUS7498026B2Fatty acid esters) is increasedEasy to calculateSugar derivativesPeptide/protein ingredientsNucleotideNucleotide sequencing
Owner:GENENCOR INT INC
Recombinant saccharomyces cerevisiae for producing dammarenediol and protopanoxadiol using xylose and construction method
The invention discloses recombinant saccharomyces cerevisiae for producing dammarenediol and protopanoxadiol using xylose and a construction method. The construction method comprises the steps of replacing a promoter of a saccharomyces cerevisiae xylulokinase gene XKS1 with a promoter PFBA1 by virtue of a homologous recombination method, introducing xylose reductase XYL1 and a xylitol dehydrogenase XYL2 expression cassette, increasing the activities of transketolase TKL1 and transaldolase TAL1 so as to obtain recombinant bacteria 1, introducing a farnesyl-diphosphate farnesyltransferase gene ERG9, a squalene monooxygenase gene ERG1 and a dammarenediol synthase gene DS into the recombinant bacteria 1 so as to obtain recombinant bacteria 2, and introducing a nicotinamide adenine dinucleotide-hydroxymethylglutaryl coenzyme A reductase gene NADH-HMGr, farnesyl diphosphatesynthase ERG20 and a protopanoxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 into the recombinant bacteria 2, so as to obtain recombinant bacteria 3. According to the recombinant saccharomyces cerevisiae, dammarenediol and protopanoxadiol can be artificially synthesized by virtue of xylose.
Owner:TIANJIN UNIV
Diacylglycerol acyl transferase 2 inhibitors
ActiveUS20180051012A1Lower capability requirementsOrganic active ingredientsSenses disorderDiseaseAcyl group
Compounds of Formula I that inhibit the activity of the diacylglycerol acyltransferase 2 (DGAT2) and their uses in the treatment of diseases linked thereto in animals are described herein.
Owner:PFIZER INC
Acyltransferases and uses thereof in fatty acid production
ActiveUS9212371B2Cosmetic preparationsOrganic active ingredientsFatty acids.polyunsaturatedTransgenesis
The present invention pertains to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.
Owner:BASF PLANT SCI GMBH
Diacylglycerol acyltransferase genes, proteins, and uses thereof
The present invention relates to diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes and proteins that exhibit both long-chain acyltransferase and acetyltransferase activity. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type transferase. The present invention also relates to methods of using diacylglycerol acyltransferase genes and proteins, including in their expression in transgenic organisms and in the production of acetyl-glycerides in plant oils, and in particular seed oils.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
Construction of isovalerylspiramycin I gene engineering strain
InactiveCN101054553AThe fermentation process is simpleSimplified regulation of the fermentation processMicroorganismsRecombinant DNA-technologyBiotechnologyAdditive ingredient
The present invention relates to use of gene engineering in modifying antibiotic ingredient, more specificly it refer to blocking and destroying 3-O-acyltransferase which take part in the production of component II and III of bitespiramycin-producing bacteria. The engineered bacteria is obtained by homologous gene double exchange and employs 4'' -isovalerylspiramycin I as main ingredient. The engineered bacteria can simplify the production process, lower the production cost and control the quality standard.
Owner:MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
Sustained release of apo a-i mimetic peptides and methods of treatment
InactiveUS20090081299A1Improve concentrationBiocidePowder deliveryReverse cholesterol transportCholesterol
A method including advancing a delivery device through a lumen of a blood vessel to a particular region in the blood vessel; and introducing a composition including a sustained-release carrier and an apolipoprotein A-I (apo A-I) synthetic mimetic peptide into a wall of the blood vessel at the particular region or a perivascular site, wherein the peptide has a property that renders the peptide effective in reverse cholesterol transport. A composition including an apolipoprotein A-I (apo A-I) synthetic peptide, or combination of an apo A-I synthetic mimetic peptide and an Acyl CoA cholesterol: acyltransferase (ACAT) inhibitor in a form suitable for delivery into a blood vessel, the peptide including an amino acid sequence in an order reverse to an order of various apo A-I mimetic peptides, or endogenous apo A-I analogs, or a chimera of helix 1 and helix 9 of endogenous apo A-I.
Owner:ABBOTT CARDIOVASCULAR
Diacylglycerol O-acyltransferase
InactiveUS20030167483A1Peptide/protein ingredientsTransferasesAcyl groupDiglyceride acyltransferase activity
Nucleic acid compositions encoding polypeptide products with diglyceride acyltransferase activity, as well as the polypeptide products encoded thereby and methods for producing the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications, as well as in treatment therapies and in the production of triacylglycerols.
Owner:THE J DAVID GLADSTONE INST A TESTAMENTARY TRUST ESTABLISHED UNDER THE WILL OF J DAVID GLADS +1
Modified photosynthetic microorganisms with reduced glycogen and their use in producing carbon-based products
This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen as compared to a wild type Cyanobacterium, and which are capable of producing an increased amount of lipids and / or fatty acids. In certain embodiments, the genetically modified photosynthetic microorganisms also contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and / or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of lipids or fatty acids and / or synthesizing triglycerides.
Owner:LUMEN BIOSCI INC
Compositions and methods for using acyltransferases for altering lipid production on the surface of plants
InactiveUS20090163729A1Increase volumeEnhancing environmental stress toleranceBryophytesOrganic chemistryLipid formationFatty acid
The present invention relates to compositions comprising acyltransferase nucleic acid molecules for altering lipids on the surface of plants, and related methods. In particular, the present invention provides compositions and methods for increasing the amount of free fatty acids, acylglycerols, and other lipids on the surface of a plant. In a preferred embodiment, the present invention relates to increasing activity of a GPAT acyltransferase for altering lipid on the plant surface, for increasing surface lipids, for enhancing environmental stress tolerance, increasing resistance to biotic stress, and providing novel plant lipids for commercial products. In further embodiments, the present invention relates to using an Arabidopsis thaliana GPAT acyltransferase for altering lipid compounds on the surface of a plant.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
N-Acetyl-(R,S)-beta-Amino Acid Acylase Gene
The present invention provides genes that encode the N-acetyl-(R,S)-β-amino acid acylases. The N-acetyl-(R,S)-β-amino acid acylases were isolated and purified from bacterial cells and the nucleotide sequences were determined. A host, such as Escherichia coli, was used to construct a high-expression system for these genes. The N-acetyl-(R)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 8. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence as shown in SEQ. ID. NO. 7. The N-acetyl-(S)-β-amino acid acylase produced by Burkholderia sp. AJ110349 (FERM BP-10366) includes, for example, the protein having the amino acid sequence shown in SEQ. ID. NO. 10. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 9. The N-acetyl-(R)-β-amino acid acylase produced by Variovorax sp. AJ110348 (FERM BP-10367) includes, for example, the protein comprised of the amino acid sequence shown in SEQ. ID. NO. 12. The gene encoding this enzyme includes, for example, the DNA having the nucleotide sequence shown inshown in SEQ. ID. NO. 11.
Owner:AJINOMOTO CO INC
Construction method of engineering strain for producing tetrahydropyrimidine by biological method
ActiveCN112961875ALow equipment requirementsAct as low temperature protectionMicroorganism based processesAcyltransferasesHeterologousReaction temperature
The invention discloses a construction method of an engineering strain for producing tetrahydropyrimidine by a biological method and application of the engineering strain in tetrahydropyrimidine production. According to the engineering strain provided by the invention, heterologous expression of an tetrahydropyrimidine synthetic gene cluster of a marine microorganism salinicola salinia is utilized, so that aminobutyric acid acetyltransferase, diaminobutyric acid aminotransferase and tetrahydropyrimidine synthetase are highly expressed in series in escherichia coli, and then sodium aspartate is catalyzed through an enzymatic reaction to generate tetrahydropyrimidine. The bioconversion method of the tetrahydropyrimidine has the characteristics of mild reaction temperature, simple process and high conversion rate.
Owner:ANHUI NORMAL UNIV
Biosynthetic gene cluster of FR901464
ActiveCN101818158AUnderstanding Biosynthetic MechanismsDepsipeptidesFermentationBiosynthetic genesGenetic engineering
The invention relates to cloning, sequencing, analysis and functional study of a biosynthetic gene cluster of a natural product FR901464 which has anti-tumor activity and is generated from pseudomonas as well as the application thereof. The whole gene cluster contains 20 genes: five polyketide synthase genes, one hybrid polyketide / non-ribosomal polypeptide synthase gene, one non-ribosomal polypeptide synthase gene, three independent acyltransferase genes, four genes related to polyketide backbone alkylation, four post-modification genes and two control-related genes. The genetic operation of the biosynthetic gene can block the biosynthesis of FR901464. The provided gene and protein thereof can be used for genetic engineering, protein expression, enzyme catalysis reaction, and the like of the compound and can also be used for searching and discovering compounds that can be used in medicines, industry or agriculture or genes and proteins thereof.
Owner:SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
Sustained release of apo a-i mimetic peptides and methods of treatment
InactiveUS20090081298A1Improve concentrationPowder deliveryPeptide/protein ingredientsSterol transportReverse cholesterol transport
A method including advancing a delivery device through a lumen of a blood vessel to a particular region in the blood vessel; and introducing a composition including a sustained-release carrier and an apolipoprotein A-I (apo A-I) synthetic mimetic peptide into a wall of the blood vessel at the particular region or a perivascular site, wherein the peptide has a property that renders the peptide effective in reverse cholesterol transport. A composition including an apolipoprotein A-I (apo A-I) synthetic peptide, or combination of an apo A-I synthetic mimetic peptide and an Acyl CoA cholesterol: acyltransferase (ACAT) inhibitor in a form suitable for delivery into a blood vessel, the peptide including an amino acid sequence in an order reverse to an order of various apo A-I mimetic peptides, or endogenous apo A-I analogs, or a chimera of helix 1 and helix 9 of endogenous apo A-I.
Owner:ABBOTT CARDIOVASCULAR
Multipath composite neuraminic acid producing bacillus subtilis and application thereof
ActiveCN111394292AHigh catalytic activityReduce synthetic pressureBacteriaTransferasesO-Phosphoric AcidGlucosaminephosphate isomerase
Owner:JIANGNAN UNIV
Plant sterol acyltransferases
The present invention is directed to lecithin:cholesterol acyltransferase-like polypeptides (LCAT) and acyl CoA:cholesterol acyltransferases-like polypeptides (ACAT). The invention provides polynucleotides encoding such cholesterol:acyltransferases-like polypeptides, polypeptides encoded by such polynucleotides, and the use of such polynucleotides to alter sterol composition and oil production in plants and host cells. Also provided are oils produced by the plants and host cells containing the polynucleotides and food products, nutritional supplements, and pharmaceutical composition containing plants or oils of the present invention. The polynucleotides of the present invention include those derived from plant sources
Owner:MONSANTO TECH LLC
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