6 results about "Transketolase" patented technology

Disclosed herein are genetically engineered yeast cells capable of producing xylitol and characterized by a deletion or disruption of a native gene encoding a glycerol-3-phosphate dehydrogenase 2a enzyme at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% identical to SEQ ID NO:107. The genetically engineered yeast cell may additionally overexpress a native glycerol kinase enzyme, express a transketolase enzyme, have a deletion or disruption of a native gene encoding an erythrose reductase, be engineered to overexpress a native RPE enzyme, express an exogenous XPDH enzyme, express an exogenous XKS enzyme, express an exogenous XDH enzyme, overexpress a native X5PP enzyme, and / or express an exogenous X5PP enzyme.

The present invention relates to the technical fields of biocatalysis and biopharmaceuticals. Disclosed is an aldehyde / ketone-initiated enzymatic synthesis method for (1R,2R)-p-methylsulfonylphenylserinol. In the method provided in the present invention, using transaminase and transketolase as catalysts, in the presence of an amino donor and an aldehyde compound or a ketone compound, a substrate p-methylsulfonylbenzaldehyde is catalyzed to produce (1R,2R)-p-methylsulfonylphenylserinol. The method provided in the present invention reduces operation steps and can realize the replacement and recycling of expensive non-industrial starting material hydroxypyruvic acid, thereby reducing production costs and thus being suitable for industrial production.

The invention discloses transketolase, transaminase and a method for preparing (1R, 2R)-1, 3-dihydroxy-2-amino-1-p-methylsulfonyl phenylpropane.The amino acid sequence of the transketolase is shown as SEQ ID NO: 6, the amino acid sequence of the transaminase is shown as SEQ ID NO: 10, the conversion rate of the transketolase used for preparing the (1R, 2R)-1, 3-dihydroxy-2-amino-1-p-methylsulfonyl phenylpropane is 99.9%, and the conversion rate of the transaminase used for preparing the (1R, 2R)-1, 3-dihydroxy-2-amino-1-p-methylsulfonyl phenylpropane is 99.9%. The method has the advantages that the de value is 97.0%, the ee value is 99.5%, the reaction cost is low, the product has high optical purity, the mode of sequentially adding enzymes along with the reaction process in a one-pot method system is adopted, the reaction system does not need to be prepared repeatedly, the operation steps are simplified, and therefore the method is beneficial to reduction of by-products and suitable for industrial production.

This invention discloses a self-initiated one-pot enzymatic synthesis method for (1R,2R)-p-methylsulfonylbenzylserine, specifically a D-serine-driven self-initiated one-pot enzymatic synthesis method for (1R,2R)-p-methylsulfonylbenzylserine, belonging to the fields of biocatalysis and biopharmaceutical technology. The method provided by this invention utilizes engineered transaminases and transketolases as catalysts, using D-serine as an amino donor to catalyze the reaction with p-methylsulfonylbenzaldehyde to produce hydroxypyruvate as an initiator, achieving efficient conversion of p-methylsulfonylbenzaldehyde to (1R,2R)-p-methylsulfonylbenzylserine. The method provided by this invention utilizes D-serine to generate hydroxypyruvate in situ and achieves the recycling of hydroxypyruvate, eliminating the need for the additional addition of expensive hydroxypyruvate (HPA) and the exogenous cofactor thiamine pyrophosphate (TPP), saving production costs and making it suitable for industrial production.

The application discloses a Yarrowia lipolytica gene engineering bacterium for producing erythritol by glycerol, and the Yarrowia lipolytica gene engineering bacterium is obtained by random insertion mutation mediated by non-homologous end connection, and strains with high cell growth and high erythritol yield are screened. Further, the gene engineering bacterium overexpresses glycerol dehydrogenase gene GCY3 or ARA1 and dihydroxyacetone kinase gene DAK2 of a glycerol metabolism dihydroxyacetone pathway. Further, the gene engineering bacterium overexpresses transketolase gene TKL1 and transaldolase gene TAL1 of a non-oxidative pentose phosphate pathway. The application further discloses a construction method and application of the gene engineering bacterium. The superior gene engineering bacterium obtained by constructing a mutant library has the characteristics of tolerating high-concentration glycerol, and the substrate conversion rate and glycerol utilization rate are significantly higher than those of a starting strain.

The invention relates to a construction method, a production method and application of a genetically engineered bacterium for synthesizing Gadusol, the genetically engineered bacterium for synthesizing Gadusol is obtained by taking escherichia coli MG1655 as an initial strain, knocking out a ketoaldolase gene on an escherichia coli MG1655 genome and cooperatively expressing an exogenous EEVS gene and an exogenous MT-Ox gene. According to the invention, an EEVS gene and a DrMT-Ox gene are integrated to a specific gene locus of an escherichia coli chromosome in a multi-copy form, and a key enzyme gene of a phosphate pentose pathway (PPP) is integrated and overexpressed on the chromosome, so that efficient biosynthesis of Gadusol is realized in escherichia coli for the first time; the method does not need exogenous plasmids, does not depend on inducers and antibiotics, can efficiently utilize a cheap carbon source, and realizes high-yield, stable and green production of Gadusol.