37 results about "Tyrosol" patented technology

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

This invention provides the medical use of tyrosol in the preparation of formulations that reduce the toxicity of penicillin-thiazolic acid (PTA). It discloses a novel medical use of tyrosol in preparing formulations that reduce PTA toxicity. By using tyrosol to prevent and treat the residual toxicity of PTA, unnecessary antibiotic replacement regimens can be avoided, significantly reducing the economic burden. A method for preventing and treating PTA residual toxicity is provided, which can significantly reduce the toxicity of PTA and has good preventive and therapeutic effects. The prevention and treatment of PTA residual toxicity has significant socio-economic implications for controlling the hygiene and quality of animal-derived foods, protecting consumer health, and ensuring industrial production.

The application provides a kind of dynamic monitoring method and system of olive leaf polyphenol components based on principal component analysis, it is related to computer technology field, comprising: obtaining original data;According to the original data, the spectrum interval is separated, and the purified polyphenol characteristic spectrum sequence is obtained;According to the purified polyphenol characteristic spectrum sequence, spectral deconvolution processing is carried out, and the polyphenol subcomponent concentration evolution matrix is obtained;According to the polyphenol subcomponent concentration evolution matrix, the principal component is reconstructed, and the reconstruction result is obtained;According to the reconstruction result, track fitting is carried out, by fitting the principal component score track into the kinetic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation step, the fitting result is obtained;According to the fitting result, prediction is carried out, the evolution end point of hydroxytyrosol free state content and its derivative relative proportion is predicted, and the dynamic monitoring result is obtained.The application realizes the accurate monitoring and prediction of the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glycoside and oleuropein in olive leaves.

The invention relates to the technical field of spectral analysis, in particular to a hydroxytyrosol content detection method and system for food quality inspection.The method comprises the steps that spectral data of an olive oil food sample are collected, liquid chromatographic data of the food sample are obtained, a peak point, a chromatographic peak sequence and a chromatographic peak half-and-half sequence are determined, a chromatographic peak shape interference coefficient is obtained, and a hydroxytyrosol content detection result is obtained. Acquiring a peak shape symmetric inclination coefficient based on the chromatographic peak shape interference coefficient and the difference before and after peak points in the chromatographic peak half-and-half sequence, determining a self-adaptive asymmetric penalty coefficient, acquiring chromatographic data of the corrected olive oil food sample, and judging whether the content of hydroxytyrosol in the olive oil food sample reaches the standard or not. The method aims at solving the problems that in a traditional BEADS algorithm, an asymmetric penalty factor cannot be dynamically adjusted according to the liquid chromatogram data of the olive oil food sample, so that the liquid chromatogram data of the olive oil food sample is excessively corrected and is not sufficiently corrected.

The application provides a method for preventing and controlling pear ring rot by using tyrosol, and the pear ring rot is prevented and controlled by spraying tyrosol aqueous solution externally; the concentration of tyrosol in the tyrosol aqueous solution is 1-5 mg / ml. The application takes 'Dangshan' pear as experimental material, and proves that tyrosol has a significant inhibitory effect on pear ring rot fungus through in-vitro antibacterial experiment and ring rot fungus inoculation experiment, can effectively inhibit the development speed of ring rot fungus spots, reduces the occurrence and spread of diseases, and thus reduces the invasion of ring rot fungus on plants. Tyrosol is a natural compound in plants, has low toxicity, and when applied to the prevention and control of pear ring rot, can enhance the defense mechanism of the plants, improve the natural resistance of the plants to ring rot, simultaneously reduces or replaces the use of chemical pesticides, and reduces the potential risks to the environment and human health.

The invention is applicable to the technical field of hydroxytyrosol preparation, and provides a method for preparing hydroxytyrosol by combining compound enzyme with molecular imprinting adsorption, which comprises the following steps: crushing fresh olive fruits, and collecting olive fruit pulp; putting the olive pulp into an enzymolysis tank, adding cellulose-pectin compound enzyme and citric acid, and performing enzymolysis; transferring the olive pulp subjected to enzymolysis into a microwave extraction tank, adding purified water, carrying out intermittent irradiation extraction, centrifuging after the extraction is completed, collecting supernate, and carrying out vacuum concentration; and adsorbing the concentrated supernate by using a chromatographic column filled with a hydroxytyrosol imprinted polymer, eluting with water and an ethanol solution in sequence, collecting ethanol eluent, and concentrating to obtain extract, namely the high-purity hydroxytyrosol. The problems that an existing fermentation method is low in yield and high in cost are solved. Generation of byproducts is reduced, and the separation and purification process is simplified; green and sustainable hydroxytyrosol industrial production is realized; and the purification period is shortened to be within 5 hours.

The invention provides an extraction method for increasing the oleuropein conversion rate and the hydroxytyrosol content in olive pomace, and belongs to the technical field of natural product extraction. Based on the analysis of the characteristics of the olive pomace material, firstly, the compact structure of the olive pomace is destroyed through steam explosion pretreatment, and pores are loosened; then carrying out compound enzyme enzymolysis, cooperatively hydrolyzing wrapped binding protein by adopting acid protease and hemicellulase in a combined manner, destroying a polysaccharide wrapping barrier in the pomace, and promoting rapid release of oleuropein; finally, high static pressure extraction is adopted, ultrahigh pressure is used for accelerating solvent permeation, the conversion rate of oleuropein is increased, and the content of hydroxytyrosol is comprehensively increased. The method is green and efficient, the oleuropein conversion rate can be increased to the maximum extent, and the content of hydroxytyrosol is increased; the degradation loss of hydroxytyrosol in the extraction process is reduced, and the economic benefits of the olive pomace are comprehensively improved.

Described herein are methods of treating a human child including administering to the human child a compound selected from epigallocatechin gallate (EGCG), or a derivative thereof selected from epicatechin (EC), epigallocatechin (RGC) and epicatechin gallate (ECG), sulforaphane, olive oil, phylloquinone, quercetin, safranal, hydroxtyrosol, menaquinone-4, menaquinone-7, oleocanthal, peptide Ins-1 B9-23 19CAM22R-E, peptide Ins-1 B12-23-19CAM, peptide Ins-1 B12-23 19CAM22R-E, or a combination of any of the foregoing, wherein administration of the treatment is started between birth and six months of age. Also described are methods of treating a pregnant human or a nursing human mother who has recently given birth to an infant. Further described are insulin B chain mimotope peptides.

The invention provides an olive leaf polyphenol component dynamic monitoring method and system based on principal component analysis, and relates to the technical field of computers, and the method comprises the following steps: obtaining original data; performing spectrum interval separation according to the original data to obtain a purified polyphenol characteristic spectrum sequence; performing spectrum deconvolution processing according to the purified polyphenol characteristic spectrum sequence to obtain a polyphenol subcomponent concentration evolution matrix; performing principal component reconstruction according to the polyphenol subcomponent concentration evolution matrix to obtain a reconstruction result; performing trajectory fitting according to a reconstruction result, and fitting a principal component score trajectory into a dynamic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation steps to obtain a fitting result; and performing prediction according to the fitting result, predicting an evolution end point of the free-state content of hydroxytyrosol and the relative proportion of the hydroxytyrosol derivative, and obtaining a dynamic monitoring result. According to the method, the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glucoside and oleuropein in the olive leaves is accurately monitored and predicted.

This invention provides a high-esterified persimmon pectin olive oil-based anti-fatigue beverage and its preparation method. The beverage comprises: extra virgin olive oil, high-esterified persimmon pectin, maltitol, freeze-dried peach powder, L-carnitine, vitamin E, hydroxytyrosol, and distilled water. The preparation process of this invention eliminates the need for processing aids such as emulsifiers and dispersants. High-esterified persimmon pectin is prepared through a pulsed electric field pretreatment-weak acid extraction process to obtain a highly active interfacial structure. This structure allows it to form a dense composite interfacial layer with other formulation components during two-stage shear emulsification, preferentially forming a stable oil-in-water emulsion system. This achieves long-term stable dispersion of the oil and water phases and masks the original spiciness and irritation of olive oil. Simultaneously, L-carnitine is added as a fatty acid metabolism support factor, and vitamin E and hydroxytyrosol are added as synergistic antioxidant components. The anti-fatigue effect is achieved through the synergistic effect of slow-release energy from the oil phase and interfacial structure regulation.

Compositions are described including a synergistic combination of hydroxytyrosol and 3-0-acetyl-11-keto-B-boswellic acid. The hydroxytyrosol may be sourced from an olive extract and the 3-0-acetyl-11-keto-B-boswellic acid may be sourced from a Boswellia serrata extract. The compositions may be formulated for oral administration to a mammalian or an avian subject. Methods for treating, repairing, or reducing damage to connective tissue caused by one or more inflammatory mediators and for reducing levels of one or more inflammatory mediators in connective tissue are provided, the methods comprising orally administering the compositions to a subject in need thereof. 20 25 20 28 75 23 A pr 2 02 5 2 4 4 2 0 6 7 4 . 1 : D C C 3 1 / 0 5 / 2 0 2 3 C O M P O S I T I O N S C O M P R I S I N G H Y D R O X Y T Y R O S O L A N D B O S W E L L I C A C I D 2 0 2 5 2 0 2 8 7 5 2 3 2 0 2 5 A p r A B S T R A C T

Stabilized oils including an edible oil and an antioxidant composition comprising α-lipoic acid and least one of ascorbic acid, ascorbyl palmitate, green tea extract, lecithin, and rosemary extract or at least one of 1,2,4-benzenetriol, carnosic acid, dihydromyricetin, dihydrorobinetin, epigallocatechin, gallic acid, 3-hydroxytyrosol, myricetin, and nepodin, and methods of preparing such stabilized edible oils. The stabilized oils may have an Oxidative Stability Index (“OSI”) at 110° C. of at least 30 hours.

The present invention discloses a composition containing tyrosol and amino acids, and its application in enhancing athletic performance and anti-fatigue. The amino acids are citrulline and / or arginine. The supplementation of tyrosol in combination with citrulline and / or arginine can increase the rate of nitric oxide production and extend the duration of nitric oxide activity, promoting vasodilation and blood flow. This, in turn, enhances athletic performance, improves endurance, and combats fatigue.

A composition comprising natural pyrethrin, one or more phenolic antioxidants selected from Group A, and a solid carrier;　wherein Group A is a group consisting of 31 specific compounds having a phenolic structure, such as 2,2'-methylenebis(6-tert-butyl-4-methylphenol), 3-hydroxytyrosol, or carnosic acid.

This invention discloses a diglyceride-based edible oil for assisting in lowering uric acid and its preparation method, relating to the field of edible oil technology. This diglyceride-based edible oil for assisting in lowering uric acid comprises the following ingredients in parts by weight: 42-50 parts modified high-oleic sunflower seed oil, 8-12 parts food-grade glycerol, 1.5-2.5 parts immobilized Rhizopus oryzae lipase, 0.3-0.8 parts modified apigenin, 0.2-0.4 parts vitamin E, 0.3-0.5 parts monoglyceride citrate, and 1.0-1.5 parts hydroxytyrosol. This composite edible oil combines the health benefits of diglycerides with the function of assisting in lowering uric acid. It exhibits good compatibility and stability of active ingredients, high bioavailability, excellent physicochemical properties, suitability for various cooking methods, and easy storage.

The application relates to a compound for improving vascular endothelial cell function, a preparation method and application thereof. The compound is prepared by taking tyrosol, dopamine, substituted cinnamic acid or 5-carboxyl pentyl-triphenyl phosphonium bromide as a starting material, and then performing amidation or esterification reaction with adamantylamine and adamantane acid. The compound has a significant inhibitory effect on the inflammation reaction of endothelial cells induced by palmitate (PA) as an anti-inflammatory molecule, can be used for preparing drugs for hyperlipidemia and related cardiovascular diseases, treating vascular endothelial injury diseases, treating hyperlipidemia, atherosclerosis and related cardiovascular diseases, and has the potential of being developed into innovative drugs for treating hyperlipidemia, atherosclerosis and related cardiovascular diseases with high efficiency and low toxicity.

The invention relates to the technical field of biology, and particularly discloses a strain of genetic engineering recombinant escherichia coli (Escherichia coli) and an application of the Escherichia coli in production of salidroside and tyrosol. The recombinant escherichia coli is obtained by overexpressing exogenous ketonic acid decarboxylase, glycosyltransferase and endogenous UDP-glucose synthesis route key enzyme in cells of an L-tyrosine production strain. The strain can be used for efficiently synthesizing salidroside from the beginning through aerobic fermentation by taking glucose as a substrate, and antibiotics and inducers do not need to be added in the fermentation process. The invention further provides a production method of the tyrosol, high-concentration salidroside is efficiently hydrolyzed to generate the tyrosol by using a provided acid hydrolysis process, and the conversion rate reaches 98%. The genetic engineering recombinant escherichia coli provided by the invention can be used for flexibly and selectively deciding to produce salidroside or tyrosol, and the production process is simple and efficient, and can be better applied to industrial production.

The present invention relates to compositions containing a mixture of three active substances, which are derived from dive extract and are hydroxytyrosol, oleuropein, and oleocanthal, said compositions being suitable for cardioprotection.

The present invention provides new classes of phenol compounds, including those derived from tyrosol and analogues, useful as monomers for preparation of biocompatible polymers, and biocompatible polymers prepared from these monomeric phenol compounds, including novel biodegradable and / or bioresorbable polymers. These biocompatible polymers or polymer compositions with enhanced bioresorbabilty and processibility are useful in a variety of medical applications, such as in medical devices and controlled-release therapeutic formulations. The invention also provides methods for preparing these monomeric phenol compounds and biocompatible polymers.

This application discloses a modified hydroxytyrosol, cosmetics, a preparation method, and applications. The provided method for preparing the modified hydroxytyrosol includes the following steps: mixing hydroxytyrosol, lipase, oleic acid, and water; adjusting the pH to acidic; reacting the mixture; centrifuging the reaction product; allowing it to separate into layers; collecting the middle layer; and obtaining the modified hydroxytyrosol. In this method, lipase is used to catalyze the modification of hydroxytyrosol with oleic acid, preparing an ester-grafted hydroxytyrosol derivative. Compared to unmodified hydroxytyrosol, the prepared modified hydroxytyrosol exhibits significantly enhanced antibacterial effects. The prepared modified hydroxytyrosol can be used as a preservative in cosmetics or food.

The application describes a large scale process for the in vitro production of an olive cell culture. The application further describes a composition in a form of a powder comprising olive fruit / leaf cells grown in vitro. The cell culture of olive cells manufactured in vitro in a large scale according to the process of the invention comprises high level of verbascoside with undetectable levels of tyrosol, hydroxytyrosol, and oleuropein.

The present invention relates to compositions containing a mixture of three active substances, which are derived from dive extract and are hydroxytyrosol, oleuropein, and oleocanthal, said compositions being suitable for cardioprotection.

The present application belongs to the field of bioengineering technology, and particularly relates to an alcohol dehydrogenase YugJ mutant and its application in fermentative production of tyrosol or hydroxytyrosol. The present application adopts a method of site-directed mutation to mutate the serine at the 41st position, the threonine at the 138th position and the valine at the 183rd position of alcohol dehydrogenase YugJ from Bacillus licheniformis into proline, serine and leucine respectively to obtain a mutant PSI (S41P / T138S / V183I). The mutant PSI improves the enzyme activity of alcohol dehydrogenase, effectively solves the problem of low alcohol performance of alcohol dehydrogenase at present, and is successfully applied to the fermentative production of tyrosol and / or hydroxytyrosol, thereby providing a new idea for improving the production level of alcohol compounds.

The invention relates to the technical field of agricultural cultivation, in particular to a method for improving low-temperature stress resistance of cherry tomato seedlings through exogenous tyrosol. The method comprises the following steps: selecting cherry tomato seedlings in a four-leaf and one-core stage, spraying the cherry tomato seedlings in an experimental group with a tyrosol aqueous solution with the concentration of 1-2.5 mg / L at 5 p.m. every day, continuously spraying for 3 days according to the standard that the leaves are covered with vaporific liquid drops without dropping, setting a normal-temperature control group and a low-temperature control group, spraying distilled water on the leaf surfaces of the cherry tomato seedlings in the control group, and observing the influence data of the tyrosol on the cherry tomatoes under the low-temperature stress. The test result proves that the exogenous tyrosol obviously relieves the growth of the cherry tomatoes under the low-temperature stress, reduces the MDA and H2O2 contents of the cherry tomato leaves, improves the contents of proline and soluble sugar, and enhances the activity of antioxidant enzyme, so that the damage of the low-temperature stress to the cherry tomato plants is effectively relieved.

This invention discloses the application of tyrosol in enhancing oxygen supply and thus improving energy supply, such as combating fatigue and improving athletic and / or cognitive performance. Enhancing oxygen supply includes increasing arterial blood oxygen saturation and / or muscle tissue oxygen saturation. Combating fatigue includes enhancing physical endurance and metabolism, reducing physical and mental fatigue, prolonging muscle and nerve working time, and / or maintaining muscle and nerve efficiency. Improving athletic performance includes enhancing the body's aerobic metabolic capacity, reducing lactic acid buildup, reducing post-exercise muscle soreness, and improving recovery speed. Improving cognitive performance includes reducing depression and anxiety; enhancing learning ability, memory, concentration, focus, reaction time, and alertness, especially in hypoxic environments and / or during exercise. Tyrosol enhances oxygen supply, thereby combating fatigue and improving athletic and cognitive performance.

The invention discloses a phenylpyruvate decarboxylase mutant and application of the phenylpyruvate decarboxylase mutant in production of tyrosol, and belongs to the technical field of bioengineering. The purpose of the present invention is to increase the yield of tyrosol. The phenylpyruvate decarboxylase mutant provided by the invention is obtained by mutating any one of the 56th site, the 92th site, the 93th site, the 106th site, the 115th site, the 153th site, the 178th site, the 188th site, the 214th site, the 220th site, the 234th site, the 242th site, the 273th site, the 285th site, the 371th site, the 416th site, the 581th site and the 621th site by taking amino acid as shown in SEQ ID NO.2 as a starting sequence. The phenylpyruvate decarboxylase is mainly used for high-efficiency phenylpyruvate decarboxylase and application thereof in de novo synthesis of tyrosol.

The invention discloses tyrosol-tolerant escherichia coli and application thereof in salidroside biosynthesis, and belongs to the technical field of genetic engineering. The 5A02 strain obtained by the invention can tolerate 40 mM tyrosol concentration, and has high tyrosol tolerance compared with an original strain 5A. The 5A02 / pkk223-3-ugt85a1 strain obtained by the invention can be used for efficiently converting tyrosol to produce the salidroside. When the strain is subjected to shake flask fermentation, the salidroside yield reaches 5.93 g / L, the large-scale fermentation yield of a 50 L fermentation tank is further increased to 24.40 g / L, and complete conversion of tyrosol is realized. The salidroside synthesis system of'tolerant strain construction + exogenous supplement of the precursor ', established by the invention, gives consideration to the synergistic advantages of de novo synthesis and precursor supply, provides a stable technical scheme for large-scale industrial production of salidroside, and has a wide industrialization prospect.

The application discloses a hydroxytyrosol and a separation and purification method thereof. The method comprises the following steps: concentrating a catalytic liquid containing hydroxytyrosol to obtain a first concentrated liquid; adding a drying aid to the first concentrated liquid, uniformly mixing to obtain a mixed liquid, and performing spray drying to obtain a powder; performing leaching on the powder, collecting a solvent phase as a second concentrated liquid, and sequentially performing thin film evaporation concentration, molecular distillation on the second concentrated liquid to obtain finished hydroxytyrosol. The method not only avoids oxidation and decomposition of the hydroxytyrosol, but also avoids water removal operation of repeatedly using a solvent, and greatly reduces the extraction cost.

The present application relates to the field of agricultural cultivation technology, and more particularly to a method for improving the low-temperature stress tolerance of cherry tomato seedlings by exogenous tyramine. The method selects cherry tomato seedlings at the 4-leaf 1-heart stage, and sprays the cherry tomato seedlings in the experimental group with a tyramine water solution at a concentration of 1-2.5 mg / L at 5 pm every day. The spraying is performed according to the standard of covering the leaves with mist droplets and no dripping. The cherry tomato seedlings in the normal temperature and low temperature control groups are sprayed with distilled water. The influence of tyramine on cherry tomatoes under low-temperature stress is observed. The test results prove that exogenous tyramine significantly alleviates the growth of cherry tomatoes under low-temperature stress, reduces the MDA and H2O2 contents in the leaves of cherry tomatoes, increases the proline and soluble sugar contents, and enhances the activity of antioxidant enzymes, thereby effectively reducing the damage of low-temperature stress to cherry tomato plants.

The invention belongs to the technical field of fibers, and discloses a wear-resistant antibacterial superfine fiber for a mop and a preparation method thereof.The fiber is prepared by taking polyvinyl alcohol as a base material and a functional additive as an additive through an electrostatic spinning technology, the functional additive is prepared by modifying the surface of nano tungsten disulfide with macromolecular nano tungsten disulfide, and the functional additive is prepared by using a high-speed electrostatic spinning technology. A large number of ether bonds contained in a polyethylene glycol structure can generate hydrogen-bond interaction with polyvinyl alcohol and then form a hydrogen-bond association network with polyvinyl alcohol, so that the compatibility of nano tungsten disulfide and polyvinyl alcohol can be effectively improved, the wear resistance of polyvinyl alcohol fibers is enhanced, and the ether bonds have good hydrophilicity and can be cooperated with polyvinyl alcohol, so that the wear resistance of the polyvinyl alcohol fibers is improved. In addition, phenolic hydroxyl groups in the hydroxytyrosol structure can be combined with protein to inhibit various enzymes necessary for growth of bacteria, so that the fiber is endowed with a good antibacterial effect.