84 results about "Tyrosol" patented technology

The invention discloses a hydroxytyrosol-ergothioneine eutectic crystal as well as a preparation method and application thereof, and belongs to the technical fields of medicines, foods, daily chemicals and the like. The invention provides a hydroxytyrosol-ergothioneine eutectic crystal. The X-ray powder diffraction pattern of the hydroxytyrosol-ergothioneine eutectic crystal has characteristic diffraction peaks at least corresponding to the positions where the 2 theta value is 4.21 degrees + / -0.2 degrees, 8.36 degrees + / -0.2 degrees, 12.29 degrees + / -0.2 degrees, 15.29 degrees + / -0.2 degrees, 19.40 degrees + / -0.2 degrees, 20.98 degrees + / -0.2 degrees, 23.12 degrees + / -0.2 degrees and 28.73 degrees + / -0.2 degrees. According to the hydroxytyrosol-ergothioneine eutectic, the melting point of hydroxytyrosol is increased, the hygroscopicity of hydroxytyrosol is remarkably reduced, and meanwhile the stability of hydroxytyrosol and ergothioneine is enhanced. In addition, after the ergothioneine hydroxytyrosol forms the eutectic crystal, the ergothioneine hydroxytyrosol can also play better anti-oxidation and anti-aging effects.

The invention relates to an anti-oxidation composition based on a reverse micelle synergistic technology and a preparation method and application thereof, the anti-oxidation composition comprises a reverse micelle structure and an aqueous solution containing an anti-oxidation active matter, and the reverse micelle structure wraps the aqueous solution containing the anti-oxidation active matter. The reverse micelle structure is used for wrapping the water-soluble antioxidant active matter, oxidation or degradation discoloration of the water-soluble antioxidant active matter is prevented, after the antioxidant active matter such as hydroxytyrosol, ergothioneine and dipotassium glycyrrhizinate is wrapped by the reverse micelle, the stability and the effect are remarkably improved, and after the reverse micelle wrapping, the effect of the antioxidant active matter is improved by 17% or above compared with an aqueous solution before wrapping.

The invention provides a grifola frondosa liquid culture medium as well as a culture method and application thereof, and relates to the technical field of fermentation. The invention provides a grifola frondosa liquid culture medium, which contains glucose, peptone, KH2P2O4, MgSO4, vitamin B1, gibberellin and tyrosol, can significantly improve the growth of grifola frondosa thalli and the secretion of exopolysaccharides, and can be used for the preparation of food, drugs and health care products.

The present invention discloses the use of tyrosol as an enhancer of the physiological effects of creatine and its application in continuous high-intensity exercise. The enhancement of physiological effects includes, but is not limited to, improves muscle endurance, reduces decline in muscle strength, enhances utilization of creatine by muscles, stimulation of the endurance-enhancing effects of creatine, reduces the decline of endurance levels, accelerates resynthesis of phosphocreatine, increases phosphocreatine levels, improves resistance performance, and enhances endurance exercise performance. Particularly, muscle endurance after continuous high-intensity exercise does not decrease or does not decrease significantly.

The invention relates to a yarrowia lipolytica strain for producing tyrosol as well as a construction method and application of the yarrowia lipolytica strain, and belongs to the technical field of genetic engineering. According to the present invention, ScADH6, ScARO10F138L and D218G genes are expressed in the yarrowia lipolytica W29 [delta] KU70; the shikimic acid feedback inhibition is relieved by expressing the Y1ARO3K225L gene, the Y1ARO4K2214 gene and the Y1AR07G139S gene; pmLAAD, EcTyrAM53I and A354V genes are introduced, so that the conversion from FAD to FADH2 is promoted, and the supply of NADH (Nicotinamide Adenine Dinucleotide Hormone) in cytoplasm is enhanced; a 4-hydroxyphenylpyruvate dioxygenase (HPD) gene is knocked out; and overexpressing Y1DHS1, Y1DHS2 and Y1DHS3 genes of a shikimic acid pathway to obtain the yarrowia lipolytica strain HY5 for producing tyrosol. The invention lays a foundation for industrial production of tyrosol, and has important social significance and economic value.

The invention discloses modified hydroxytyrosol, flavored hydroxytyrosol, a preparation method of the modified hydroxytyrosol, a preparation method of the flavored hydroxytyrosol, functional food and cosmetics. Activated carbon loaded p-toluenesulfonic acid is used as a catalyst to catalyze papain to modify hydroxytyrosol, so that the effect activity of the modified hydroxytyrosol raw material is reserved, and meanwhile, the bad flavor generated by impurities in industrial production of the hydroxytyrosol raw material is remarkably reduced; furthermore, the smell and the flavor of the hydroxytyrosol raw material are remarkably improved, the application effect of the hydroxytyrosol raw material in related fields is improved, and the use sensory experience of the product is improved. The flavor of hydroxytyrosol is improved in an enzyme preparation catalysis mode, and the process method is green and safe.

This invention provides the medical use of tyrosol in the preparation of formulations that reduce the toxicity of penicillin-thiazolic acid (PTA). It discloses a novel medical use of tyrosol in preparing formulations that reduce PTA toxicity. By using tyrosol to prevent and treat the residual toxicity of PTA, unnecessary antibiotic replacement regimens can be avoided, significantly reducing the economic burden. A method for preventing and treating PTA residual toxicity is provided, which can significantly reduce the toxicity of PTA and has good preventive and therapeutic effects. The prevention and treatment of PTA residual toxicity has significant socio-economic implications for controlling the hygiene and quality of animal-derived foods, protecting consumer health, and ensuring industrial production.

The application provides a kind of dynamic monitoring method and system of olive leaf polyphenol components based on principal component analysis, it is related to computer technology field, comprising: obtaining original data;According to the original data, the spectrum interval is separated, and the purified polyphenol characteristic spectrum sequence is obtained;According to the purified polyphenol characteristic spectrum sequence, spectral deconvolution processing is carried out, and the polyphenol subcomponent concentration evolution matrix is obtained;According to the polyphenol subcomponent concentration evolution matrix, the principal component is reconstructed, and the reconstruction result is obtained;According to the reconstruction result, track fitting is carried out, by fitting the principal component score track into the kinetic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation step, the fitting result is obtained;According to the fitting result, prediction is carried out, the evolution end point of hydroxytyrosol free state content and its derivative relative proportion is predicted, and the dynamic monitoring result is obtained.The application realizes the accurate monitoring and prediction of the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glycoside and oleuropein in olive leaves.

The invention relates to the technical field of spectral analysis, in particular to a hydroxytyrosol content detection method and system for food quality inspection.The method comprises the steps that spectral data of an olive oil food sample are collected, liquid chromatographic data of the food sample are obtained, a peak point, a chromatographic peak sequence and a chromatographic peak half-and-half sequence are determined, a chromatographic peak shape interference coefficient is obtained, and a hydroxytyrosol content detection result is obtained. Acquiring a peak shape symmetric inclination coefficient based on the chromatographic peak shape interference coefficient and the difference before and after peak points in the chromatographic peak half-and-half sequence, determining a self-adaptive asymmetric penalty coefficient, acquiring chromatographic data of the corrected olive oil food sample, and judging whether the content of hydroxytyrosol in the olive oil food sample reaches the standard or not. The method aims at solving the problems that in a traditional BEADS algorithm, an asymmetric penalty factor cannot be dynamically adjusted according to the liquid chromatogram data of the olive oil food sample, so that the liquid chromatogram data of the olive oil food sample is excessively corrected and is not sufficiently corrected.

The application discloses a kind of flame-retardant bio-based copolymer resin based on PT-fa, to obtain mixed resin with bio-based benzoxazine monomer PT-fa and bisphenol A-aniline type benzoxazine BA-a as raw material, then, mixed resin is heat cured to obtain flame-retardant bio-based copolymer resin Poly (PT-fa / BA-a) based on PT-fa, the obtained copolymer resin has high thermal stability and high flame retardant performance;The PT-fa is prepared by solvent-free synthesis method with hydroxyl tyrosol, furfurylamine and polyformaldehyde as raw material, the obtained monomer has high purity.The glass transition temperature T g of the Poly (PT-fa / BA-a) is 180-300 DEG C;The temperature T di of 5% thermal weight loss under nitrogen atmosphere is 328-340 DEG C, the carbon residue rate Y c at 800 DEG C is 38-60%;Heat release capacity HRC is 80-175 J / g·K, and the total heat release THR is 10-21 kJ / g.The preparation method comprises the following steps: 1, the preparation of bio-based benzoxazine monomer PT-fa;2, the preparation of flame-retardant bio-based copolymer resin based on PT-fa.

The present invention relates to the field of biogenic nitrogen fertilizer synergists, and in particular to an application of an olive pomace polyphenol extract-hydroxytyrosol as a biogenic nitrogen fertilizer synergist. The biogenic nitrogen fertilizer synergist component prepared by the present invention is an olive pomace polyphenol extract-hydroxytyrosol. After the olive pomace is deoiled, it is extracted under acid hydrolysis conditions, and an alkaline solution of sodium hydroxide is used to adjust the pH value to neutral to obtain the corresponding hydroxytyrosol. The abundance of soil nitrifying microorganisms (ammonia oxidizing bacteria and ammonia oxidizing archaea) and the conversion of mineral nitrogen in the soil can be effectively suppressed, the effectiveness of nitrogen in the soil can be improved, nitrogen can be more effectively absorbed and utilized by crops, the olive pomace is effectively recycled, and the problems of huge environmental pollution and waste of resources caused by the olive pomace can be effectively avoided.

The invention belongs to the technical field of medicines, and particularly relates to application of tyrosol in preparation of a medicine for preventing and treating radioactive intestinal injury. The CAS number of the tyrosol provided by the invention is 501-94-0, and SMILES is C1 = CC (= CC = C1CCO) O; the radioactive intestinal injury is intestinal injury caused by ionizing radiation and comprises acute radioactive intestinal injury and chronic radioactive intestinal injury. An animal model is utilized, it is proved from the cellular level that the tyrosol can be used for preventing and treating the radioactive intestinal injury, safety is high, the tyrosol is suitable for acute radiation disease patients, nuclear employees, astronauts, radiotherapy patients and the like, the tyrosol can be used before treatment or during task execution, and a new medicine choice is provided for preventing and treating the radioactive intestinal injury.

The invention discloses temperature-induced Escherichia coli for high yield of tyrosol and hydroxytyrosol as well as a construction method and application of the temperature-induced Escherichia coli, and belongs to the technical field of microbial synthesis. On the basis of escherichia coli with high tyrosine yield, a temperature induction system is utilized for the first time, AroGfbr, ARO10, ADHs and TyrAfbr genes are over-expressed, and de novo synthesis of tyrosol is achieved; and screening ARO10 and ADHs genes to obtain the strain with high yield of tyrosol. On the basis, an HpaB / C gene is over-expressed, meanwhile, pykA, tyrB and mhpB genes are knocked out on a genome, glf and glk genes are knocked into a ptsG site, the yield of hydroxytyrosol is remarkably increased, the shake flask yield reaches 1.28 g / L, and the yield of a 5L reactor reaches 4.97 g / L. The method lays a foundation for low-cost and high-efficiency industrial production of tyrosol and hydroxytyrosol, and has a wide application prospect.

The present application relates to a kind of selectively improved glycosyltransferase UGTBL1 mutant and its catalytic synthesis of application of salidroside, belong to genetic engineering and enzyme engineering field.The mutant obtained by single point mutation technology includes the amino acid sequence of 132th amino mutation, the amino acid sequence of the glycosyltransferase is as shown in SEQ ID NO:1.The research results show that the glycosyltransferase mutant S132V, S132G described in the application has good selectivity, can efficiently catalyze tyrosol and uridine diphosphate glucose to synthesize salidroside, and the selectivity is 89%, suitable for the enzyme preparation of salidroside, with wide application prospect.

The application provides a method for preventing and controlling pear ring rot by using tyrosol, and the pear ring rot is prevented and controlled by spraying tyrosol aqueous solution externally; the concentration of tyrosol in the tyrosol aqueous solution is 1-5 mg / ml. The application takes 'Dangshan' pear as experimental material, and proves that tyrosol has a significant inhibitory effect on pear ring rot fungus through in-vitro antibacterial experiment and ring rot fungus inoculation experiment, can effectively inhibit the development speed of ring rot fungus spots, reduces the occurrence and spread of diseases, and thus reduces the invasion of ring rot fungus on plants. Tyrosol is a natural compound in plants, has low toxicity, and when applied to the prevention and control of pear ring rot, can enhance the defense mechanism of the plants, improve the natural resistance of the plants to ring rot, simultaneously reduces or replaces the use of chemical pesticides, and reduces the potential risks to the environment and human health.

The invention is applicable to the technical field of hydroxytyrosol preparation, and provides a method for preparing hydroxytyrosol by combining compound enzyme with molecular imprinting adsorption, which comprises the following steps: crushing fresh olive fruits, and collecting olive fruit pulp; putting the olive pulp into an enzymolysis tank, adding cellulose-pectin compound enzyme and citric acid, and performing enzymolysis; transferring the olive pulp subjected to enzymolysis into a microwave extraction tank, adding purified water, carrying out intermittent irradiation extraction, centrifuging after the extraction is completed, collecting supernate, and carrying out vacuum concentration; and adsorbing the concentrated supernate by using a chromatographic column filled with a hydroxytyrosol imprinted polymer, eluting with water and an ethanol solution in sequence, collecting ethanol eluent, and concentrating to obtain extract, namely the high-purity hydroxytyrosol. The problems that an existing fermentation method is low in yield and high in cost are solved. Generation of byproducts is reduced, and the separation and purification process is simplified; green and sustainable hydroxytyrosol industrial production is realized; and the purification period is shortened to be within 5 hours.

The invention provides an extraction method for increasing the oleuropein conversion rate and the hydroxytyrosol content in olive pomace, and belongs to the technical field of natural product extraction. Based on the analysis of the characteristics of the olive pomace material, firstly, the compact structure of the olive pomace is destroyed through steam explosion pretreatment, and pores are loosened; then carrying out compound enzyme enzymolysis, cooperatively hydrolyzing wrapped binding protein by adopting acid protease and hemicellulase in a combined manner, destroying a polysaccharide wrapping barrier in the pomace, and promoting rapid release of oleuropein; finally, high static pressure extraction is adopted, ultrahigh pressure is used for accelerating solvent permeation, the conversion rate of oleuropein is increased, and the content of hydroxytyrosol is comprehensively increased. The method is green and efficient, the oleuropein conversion rate can be increased to the maximum extent, and the content of hydroxytyrosol is increased; the degradation loss of hydroxytyrosol in the extraction process is reduced, and the economic benefits of the olive pomace are comprehensively improved.

Described herein are methods of treating a human child including administering to the human child a compound selected from epigallocatechin gallate (EGCG), or a derivative thereof selected from epicatechin (EC), epigallocatechin (RGC) and epicatechin gallate (ECG), sulforaphane, olive oil, phylloquinone, quercetin, safranal, hydroxtyrosol, menaquinone-4, menaquinone-7, oleocanthal, peptide Ins-1 B9-23 19CAM22R-E, peptide Ins-1 B12-23-19CAM, peptide Ins-1 B12-23 19CAM22R-E, or a combination of any of the foregoing, wherein administration of the treatment is started between birth and six months of age. Also described are methods of treating a pregnant human or a nursing human mother who has recently given birth to an infant. Further described are insulin B chain mimotope peptides.

The invention provides an olive leaf polyphenol component dynamic monitoring method and system based on principal component analysis, and relates to the technical field of computers, and the method comprises the following steps: obtaining original data; performing spectrum interval separation according to the original data to obtain a purified polyphenol characteristic spectrum sequence; performing spectrum deconvolution processing according to the purified polyphenol characteristic spectrum sequence to obtain a polyphenol subcomponent concentration evolution matrix; performing principal component reconstruction according to the polyphenol subcomponent concentration evolution matrix to obtain a reconstruction result; performing trajectory fitting according to a reconstruction result, and fitting a principal component score trajectory into a dynamic network model containing hydroxytyrosol glycoside hydrolysis and oxidative condensation steps to obtain a fitting result; and performing prediction according to the fitting result, predicting an evolution end point of the free-state content of hydroxytyrosol and the relative proportion of the hydroxytyrosol derivative, and obtaining a dynamic monitoring result. According to the method, the dynamic conversion process of specific polyphenol components such as hydroxytyrosol glucoside and oleuropein in the olive leaves is accurately monitored and predicted.

The application discloses a preparation method of deodorizing anion dopamine modified graphene quantum dot cloud wool, and relates to the technical field of cloud wool.The hydroxyl tyrosol 3-sodium sulfate salt and sebacic acid are used in polyester polymerization reaction to prepare elastic polyester particles, so that the deformation resistance of the cloud wool is improved; meanwhile, the sulfate radical has the effects of antistatic and antibacterial; finally, the polyester chip is used as a core layer, the elastic polyester particles, dopamine modified graphene quantum dots and modified antibacterial agent are used as a skin layer, and melt spinning is carried out to prepare the deodorizing anion dopamine modified graphene quantum dot cloud wool, so that the effects of antibacterial, deodorization and antistatic are realized.The modified antibacterial agent uses 1,6-dibromoisoquinoline-3-amine and N,N,N',N'-tetramethyl-2-butene-1,4-diamine to form a double quaternary ammonium salt structure, so that the antibacterial effect of the cloud wool is improved, the elastic polyester particles are assisted, and the deodorization effect is improved; finally, tea polyphenol is added in the melt spinning process, a three-dimensional structure is formed, the skin layer protection capability is improved, and the deformation resistance is improved.

This invention provides a high-esterified persimmon pectin olive oil-based anti-fatigue beverage and its preparation method. The beverage comprises: extra virgin olive oil, high-esterified persimmon pectin, maltitol, freeze-dried peach powder, L-carnitine, vitamin E, hydroxytyrosol, and distilled water. The preparation process of this invention eliminates the need for processing aids such as emulsifiers and dispersants. High-esterified persimmon pectin is prepared through a pulsed electric field pretreatment-weak acid extraction process to obtain a highly active interfacial structure. This structure allows it to form a dense composite interfacial layer with other formulation components during two-stage shear emulsification, preferentially forming a stable oil-in-water emulsion system. This achieves long-term stable dispersion of the oil and water phases and masks the original spiciness and irritation of olive oil. Simultaneously, L-carnitine is added as a fatty acid metabolism support factor, and vitamin E and hydroxytyrosol are added as synergistic antioxidant components. The anti-fatigue effect is achieved through the synergistic effect of slow-release energy from the oil phase and interfacial structure regulation.

The present invention discloses a glycosyltransferase derived from Camelina sativa and its application. The amino acid sequence of the UDP-glycosyltransferase disclosed in the present invention is shown in SEQ ID NO:1, and the amino acid sequence of its mutant is shown in SEQ ID NO:3. The UDP-glycosyltransferase and its mutant provided by the present invention can efficiently catalyze the conversion of tyrosol to salidroside, and its mutant has good substrate tolerance, which is of great significance for the production and application of salidroside.

The present invention discloses a composition containing tyrosol and amino acids, and its application in enhancing athletic performance and anti-fatigue. The amino acids are citrulline and / or arginine. The supplementation of tyrosol in combination with citrulline and / or arginine can increase the rate of nitric oxide production and extend the duration of nitric oxide activity, promoting vasodilation and blood flow. This, in turn, enhances athletic performance, improves endurance, and combats fatigue.

Compositions comprising hydroxytyrosol and masticinic acid are provided. Compositions comprising a synergistic combination of hydroxytyrosol and 3-O-acetyl-11-one-beta-masticinic acid are described. The hydroxytyrosol can be derived from an olive extract, and the 3-O-acetyl-11-keto-beta-boswellic acid can be derived from a Boswellia serrata extract, and the 3-O-acetyl-11-keto-beta-boswellic acid can be derived from a Boswellia serrata extract. The composition may be formulated for oral administration to a mammalian or avian subject. Methods are provided for treating, repairing, or reducing connective tissue damage caused by one or more inflammatory mediators, and reducing the level of one or more inflammatory mediators in connective tissue, comprising orally administering the composition to a subject in need thereof.

The present invention relates to the field of pharmaceutical technology, specifically to hydroxytyrosol nicotinamide cocrystal, its preparation method and composition. The molar ratio of hydroxytyrosol and nicotinamide in the hydroxytyrosol nicotinamide cocrystal of the present invention is 1:1, and the cell parameters are a=9.4999, b=11.8285, c=11.4439, α=90°, β=96.628°, δ=90°. The hydroxytyrosol nicotinamide cocrystal of the present invention has a high melting point, is not hygroscopic, has good stability, and improves the convenience of use of hydroxytyrosol. The composition directly prepared by grinding method has a high yield, low cost, and is suitable for large-scale wide application.

Compositions are described including a synergistic combination of hydroxytyrosol and 3-0-acetyl-11-keto-B-boswellic acid. The hydroxytyrosol may be sourced from an olive extract and the 3-0-acetyl-11-keto-B-boswellic acid may be sourced from a Boswellia serrata extract. The compositions may be formulated for oral administration to a mammalian or an avian subject. Methods for treating, repairing, or reducing damage to connective tissue caused by one or more inflammatory mediators and for reducing levels of one or more inflammatory mediators in connective tissue are provided, the methods comprising orally administering the compositions to a subject in need thereof. 20 25 20 28 75 23 A pr 2 02 5 2 4 4 2 0 6 7 4 . 1 : D C C 3 1 / 0 5 / 2 0 2 3 C O M P O S I T I O N S C O M P R I S I N G H Y D R O X Y T Y R O S O L A N D B O S W E L L I C A C I D 2 0 2 5 2 0 2 8 7 5 2 3 2 0 2 5 A p r A B S T R A C T

Stabilized oils including an edible oil and an antioxidant composition comprising α-lipoic acid and least one of ascorbic acid, ascorbyl palmitate, green tea extract, lecithin, and rosemary extract or at least one of 1,2,4-benzenetriol, carnosic acid, dihydromyricetin, dihydrorobinetin, epigallocatechin, gallic acid, 3-hydroxytyrosol, myricetin, and nepodin, and methods of preparing such stabilized edible oils. The stabilized oils may have an Oxidative Stability Index (“OSI”) at 110° C. of at least 30 hours.

The present invention discloses a composition containing tyrosol and amino acids, and its application in enhancing athletic performance and anti-fatigue. The amino acids are citrulline and / or arginine. The supplementation of tyrosol in combination with citrulline and / or arginine can increase the rate of nitric oxide production and extend the duration of nitric oxide activity, promoting vasodilation and blood flow. This, in turn, enhances athletic performance, improves endurance, and combats fatigue.

The present application provides a grifola frondosa liquid culture medium and its culture method and application, relates to the fermentation technical field.The present application provides a grifola frondosa liquid culture medium, including glucose, proteose peptone, KH2PO4, MgSO4, vitamin B1, gibberellin and tyrosol, the culture medium can significantly improve grifola frondosa growth and the secretion of extracellular polysaccharide, and can be used for the preparation of food, medicine and health care product.

A composition comprising natural pyrethrin, one or more phenolic antioxidants selected from Group A, and a solid carrier;　wherein Group A is a group consisting of 31 specific compounds having a phenolic structure, such as 2,2'-methylenebis(6-tert-butyl-4-methylphenol), 3-hydroxytyrosol, or carnosic acid.