7 results about "Yarrowia" patented technology

The present invention relates to mutants of the bumblebee Yarrowia SBLE enzyme having modulated transesterification and / or esterification activity compared to the bumblebee Yarrowia SBLE enzyme, and to homologues of the bumblebee Yarrowia SBLE enzyme, such as the C. bogoriensis LE enzyme, the B. batistae LE enzyme and the R. riograndensis LE enzyme, having transesterification and esterification activity on bolaform glycolipids. The present invention also provides genetically modified yeast strains comprising a mutant LE enzyme or a bumblebee Yarrowia SBLE enzyme homologue of the present invention. The present invention further provides the use and methods of the mutant LE enzymes, the bumblebee Yarrowia SBLE enzyme homologues and the modified yeast strains of the present invention for the production of acidic glycolipids, lactone-form glycolipids and / or glycolipid oligomers / polymers / esters. The present invention also provides the three-dimensional structure of the bumblebee Yarrowia SBLE enzyme and its use for the design of mutants having modulated hydrolysis and / or transesterification activity.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

This invention discloses a high-yielding erythritol-producing *Yarrowia lipophila* strain (… Yarrowia lipolytica This invention relates to the field of biotechnology, specifically to *Yarrowia lipophila* CA20 as the starting strain, and its applications. A000039 A gene knockout method was used to construct a high-yield erythritol-producing *Yarrowia lipolytica* strain. The construction method provided by this invention is simple and of significant value in simplifying the construction process of high-yield strains. Furthermore, under the fermentation medium and conditions provided by this invention, the *Yarrowia lipolytica* strain producing high-yield erythritol was fed-batch fermented with glucose as a substrate, achieving a yield of 280.50 g / L and a yield of 0.71 g / g, demonstrating good economic benefits.

This invention belongs to the field of vinegar fermentation and processing technology, specifically relating to a medicinal and edible vinegar for improving postprandial blood sugar and its segmented fermentation method. The medicinal and edible vinegar for improving postprandial blood sugar is made from highland barley and a compound powder of medicinal and edible ingredients. The segmented fermentation method includes: adding brewing yeast to highland barley and fermenting to obtain fermentation mash; adding the compound powder of medicinal and edible ingredients to the fermentation mash and performing coupled fermentation; subsequently inoculating with acetic acid bacteria for acetic acid fermentation; and adding functional strains such as *Yarrowia spp.* for targeted metabolism of residual sugars in the middle stage; finally, after post-treatment, the medicinal and edible vinegar for improving postprandial blood sugar is obtained. The segmented fermentation method of this invention can significantly improve the retention rate of active ingredients and fermentation stability, giving the resulting medicinal and edible vinegar for improving postprandial blood sugar the advantages of natural low sugar, mellow flavor, and high retention rate of active ingredients. It is suitable for the industrial production of low-sugar, highly stable medicinal and edible health vinegar.

The application relates to the fermentation field and discloses a Yarrowia lipolytica and application thereof. The preservation number of the Yarrowia lipolytica is CGMCC No. 23718. The Yarrowia lipolytica has high fermentation stability, high tolerance to high temperature and high sugar concentration, and still has good erythritol production capacity under the conditions of high temperature and high initial sugar concentration; the Yarrowia lipolytica is used for producing erythritol, batch fermentation can be carried out with high initial sugar concentration, high content of erythritol is obtained, by-products in the fermentation are few, and the amount of cooling water or refrigerated water used for temperature control in the fermentation process can be reduced.