23 results about "Yarrowia" patented technology

The invention provides a genetically engineered bacterium for producing astaxanthin, and the genetically engineered bacterium takes Yarrowia lipolytica Po1f as an original strain and contains a GGS gene, an HMGR gene, a beta-carotene synthesis gene, a beta-carotene ketolase gene and a beta-carotene hydroxylase gene. The invention further provides the Yarrowia lipolytica MC247, and the preservation number of the Yarrowia lipolytica MC247 is CGMCC NO.29699. According to the Yarrowia lipolytica MC247, the high-yield astaxanthin is obtained, a new technical scheme is provided for efficient and green manufacturing of the astaxanthin, and industrial production of the astaxanthin is facilitated.

The present invention relates to mutants of the bumblebee Yarrowia SBLE enzyme having modulated transesterification and / or esterification activity compared to the bumblebee Yarrowia SBLE enzyme, and to homologues of the bumblebee Yarrowia SBLE enzyme, such as the C. bogoriensis LE enzyme, the B. batistae LE enzyme and the R. riograndensis LE enzyme, having transesterification and esterification activity on bolaform glycolipids. The present invention also provides genetically modified yeast strains comprising a mutant LE enzyme or a bumblebee Yarrowia SBLE enzyme homologue of the present invention. The present invention further provides the use and methods of the mutant LE enzymes, the bumblebee Yarrowia SBLE enzyme homologues and the modified yeast strains of the present invention for the production of acidic glycolipids, lactone-form glycolipids and / or glycolipid oligomers / polymers / esters. The present invention also provides the three-dimensional structure of the bumblebee Yarrowia SBLE enzyme and its use for the design of mutants having modulated hydrolysis and / or transesterification activity.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The application discloses a process for producing resveratrol by fermentation of Yarrowia lipolytica, and belongs to the technical field of microorganisms. Through a series of fermentation condition optimization, including adjustment of the basic medium composition, concentration, feeding composition, pH and feeding mode. The fermentation yield after adjustment is increased from 6g / L at the beginning to 22g / L, and the purity of the extracted resveratrol is greater than 80%, which solves the problems of insufficient yield, difficult extraction and low purity compared with resveratrol from plants.

The invention relates to the technical field of genetic engineering and bioengineering, in particular to a Rhodococcus lipolytica engineering bacterium and application thereof in preparation of sclareol, the Rhodococcus lipolytica engineering bacterium comprises SsLPPS, SsSCS, nucleotide for encoding polypeptide interaction tags RIAD and RIDD, and the nucleotide for encoding polypeptide interaction tags RIAD and RIDD is used for encoding polypeptide interaction tags RIAD and RIDD. The Yarrowia lipolytica engineering bacterium overexpresses an MVA pathway key gene and a GGPP synthesis related gene, and the MVA pathway key gene is selected from erg8, erg10 and erg20; the GGPP synthesis related gene is selected from tPaGGPPS and / or SsGGPPS, and the GGPP synthesis related gene is selected from the group consisting of tPaGGPPS and / or The promoter of the erg9 gene of the Yarrowia lipolytica engineering bacterium is a truncated promoter, and the length of the promoter is 40-110 bp. According to the method, the sclareol is efficiently synthesized in the Yarrowia lipolytica, and the final shake flask yield reaches 1.5 g / L.

Yarrowia lipolytica Yl590 and the use thereof. The Yarrowia lipolytica Yl590, taxonomically classified as Yarrowia lipolytica, was deposited with the China General Microbiological Culture Collection Center on 4 June 2024, with a deposit address of Beijing, China and a deposit number of CGMCC No. 30855. The present invention can solve the problem of the slow growth rate of Yarrowia lipolytica in the prior art, and is suitable for the technical field of microorganisms.

The invention relates to a process for preparing alpha-ketoglutaric acid based on citrus fermentation liquor, which comprises the following steps: inoculating AKG-producing arrowia lipolytica seed solution into the prepared citrus fermentation liquor, regulating and controlling the dissolved oxygen concentration and pH value in stages in a fermentation period, and adding a fermentation regulator, thereby obtaining the alpha-ketoglutaric acid. AKG fermentation liquor is sequentially subjected to centrifugation, membrane filtration and continuous chromatographic separation and purification, AKG seed crystals are added for induced crystallization to obtain an AKG finished product, a metabolic network of the arrowia lipolytica is accurately regulated and controlled in a dynamic fermentation mode of dissolved oxygen step control, pH stage control and fermentation regulator supplementing, the concentration of the AKG in the fermentation liquor is increased, and the yield of the AKG is increased. The method provided by the invention has the advantages of simple process and low cost, by-product accumulation is reduced, a multi-step synergistic separation and purification mode of pretreatment, membrane filtration and continuous chromatographic separation in the separation and purification stage is matched, fermentation products can be efficiently recovered and purified, and the separation yield and the purity of an AKG finished product are improved, so that efficient and green preparation of AKG is realized, and the method has extremely high industrial application value.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

This invention discloses a high-yielding erythritol-producing *Yarrowia lipophila* strain (… Yarrowia lipolytica This invention relates to the field of biotechnology, specifically to *Yarrowia lipophila* CA20 as the starting strain, and its applications. A000039 A gene knockout method was used to construct a high-yield erythritol-producing *Yarrowia lipolytica* strain. The construction method provided by this invention is simple and of significant value in simplifying the construction process of high-yield strains. Furthermore, under the fermentation medium and conditions provided by this invention, the *Yarrowia lipolytica* strain producing high-yield erythritol was fed-batch fermented with glucose as a substrate, achieving a yield of 280.50 g / L and a yield of 0.71 g / g, demonstrating good economic benefits.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing abscisic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, heterologous synthesis of ABA is realized in the Yarrowia lipolytica, precursor supply is optimized by enhancing carbon flow in a metabolic pathway, the activity of key enzymes BcABA2 and BcABA3 is improved by adopting a semi-rational strategy, NADPH regeneration engineering is implemented to enhance the oxygen uptake ability of a strain, a cytoplasm-peroxisome engineering system is constructed, and the oxygen uptake ability of the strain is enhanced. The ABA synthesis is further enhanced by utilizing the characteristic of high-yield acetyl coenzyme A of peroxisome. According to the invention, fermentation conditions are further optimized, so that the ABA accumulation concentration of the constructed engineering strain in a 5 L bioreactor reaches 2554.36 mg / L, and the highest titer of ABA biosynthesis reported so far is created. The metabolic modification strategy provides important reference for biosynthesis of other terpenoids.

The present invention discloses flavonoid 4'-O-methyltransferase and its application in the production of hesperetin, belonging to the field of genetic engineering and bioengineering technology. The present invention screened and identified new flavonoid 4'-O-methyltransferases derived from tea-branch orange and Huazhou pomelo; the flavonoid 4'-O-methyltransferases CrcOMT-2 and CgtOMT-3 obtained by screening were integrated and expressed in the multi-copy site rDNA and ZETA site on the genome of Yarrowia lipolytica YE26, respectively, to achieve the de novo synthesis of hesperetin. The Yarrowia lipolytica Z02 constructed by the present invention achieved the de novo synthesis of 130.2 mg / L hesperetin in a 5L fermentor, which is also the highest yield of hesperetin de novo synthesis in microorganisms reported, laying the foundation for the subsequent biosynthesis of flavonoids.

The present application relates to the preparation process of the lysate product of bifidobacterium fermentation honey and extract of yarrow, comprising the preparation of bifidobacterium activation medium; strain liquid propagation; composition preparation; anaerobic fermentation culture; finally, the fermentation broth of the above composition is inactivated by low-temperature micro-jet, centrifuged, and the supernatant is obtained, and the lysate product of bifidobacterium is obtained. Compared with the blank culture medium, the total number of viable bacteria and OD value can be obviously improved by adding glucose or honey as carbon source, and when the carbon source is further selected as honey, the total number of viable bacteria after fermentation culture by the culture medium is more, and the OD value is higher.

This invention belongs to the field of vinegar fermentation and processing technology, specifically relating to a medicinal and edible vinegar for improving postprandial blood sugar and its segmented fermentation method. The medicinal and edible vinegar for improving postprandial blood sugar is made from highland barley and a compound powder of medicinal and edible ingredients. The segmented fermentation method includes: adding brewing yeast to highland barley and fermenting to obtain fermentation mash; adding the compound powder of medicinal and edible ingredients to the fermentation mash and performing coupled fermentation; subsequently inoculating with acetic acid bacteria for acetic acid fermentation; and adding functional strains such as *Yarrowia spp.* for targeted metabolism of residual sugars in the middle stage; finally, after post-treatment, the medicinal and edible vinegar for improving postprandial blood sugar is obtained. The segmented fermentation method of this invention can significantly improve the retention rate of active ingredients and fermentation stability, giving the resulting medicinal and edible vinegar for improving postprandial blood sugar the advantages of natural low sugar, mellow flavor, and high retention rate of active ingredients. It is suitable for the industrial production of low-sugar, highly stable medicinal and edible health vinegar.

The present application relates to: a Yarrowia genus microorganism having a retinol-producing ability, in which the activity of an ABC3 protein is reduced as compared to the endogenous activity thereof; a retinol production method using the same; a method for producing a microorganism of the genus Yarrowia having a retinol-producing ability; the invention discloses a method for enhancing retinol release of microorganisms. And a composition for producing retinol.

The application discloses a Yarrowia lipolytica, a preparation, application and a preparation method of erythritol, and belongs to the technical field of microorganisms. Yarrowia lipolytica The Yarrowia lipolytica is preserved in the China General Microbiological Culture Collection Center on December 26, 2022, and the preservation number is CGMCC No. 26283. The Yarrowia lipolytica Yarrowia lipolytica The Yarrowia lipolytica has simple culture conditions, high temperature resistance (35 DEG C), a glucose-to-erythritol conversion rate of 71%, high erythritol yield, few by-products, high yield, easy separation and purification of products, and can effectively reduce the production cost of erythritol in industrial production.

The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to a method for biologically synthesizing SNAC, which comprises the following steps: inoculating yeast to a fermentation medium, adding 2-hydroxy-N-octyl benzamide and a salt-forming agent, controlling the pH value of the fermentation medium to be alkaline, and carrying out biological fermentation to synthesize the SNAC, wherein the yeast is selected from one of candida tropicalis, candida maltose and yarrowia lipolytica. According to the method, a biological method and a chemical method are organically fused, respective advantages of the biological method and the chemical method are fully played, a brand new SNAC synthesis route mainly depending on microbial fermentation is innovatively developed, green synthesis of the SNAC is realized by utilizing a microbial cell factory, the synthesis route of the SNAC is greatly shortened, and the method has the advantages of cheap and easily available raw materials, lower cost, mild reaction conditions, high yield and the like. And the method has the advantages of low environmental pollution, high atom economy, green production and the like.

The application relates to the fermentation field and discloses a Yarrowia lipolytica and application thereof. The preservation number of the Yarrowia lipolytica is CGMCC No. 23718. The Yarrowia lipolytica has high fermentation stability, high tolerance to high temperature and high sugar concentration, and still has good erythritol production capacity under the conditions of high temperature and high initial sugar concentration; the Yarrowia lipolytica is used for producing erythritol, batch fermentation can be carried out with high initial sugar concentration, high content of erythritol is obtained, by-products in the fermentation are few, and the amount of cooling water or refrigerated water used for temperature control in the fermentation process can be reduced.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing ergosterol and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the method, sterol synthesis route genes are screened, the speed limiting step is determined, high-yield ergosterol is preliminarily achieved through combined expression, the flow direction of carbon metabolic flow is further pulled through lipid droplet engineering and cell area chamber engineering, and the yield of ergosterol is increased. On the basis of enzyme modification engineering, the catalytic efficiency of the ERG11 on the lanosterol is improved through substrate channel engineering and proton-dependent catalytic path construction. Through multi-copy integration of the genes in the speed limiting step, the yield of ergosterol in a shake flask of the constructed engineering strain reaches 433.1 mg / L, and the yield of ergosterol in a 5L fermentation tank system reaches 4.58 g / L.

The application discloses a Yarrowia lipolytica strain for synthesizing delta-tocotrienol and an application thereof, and belongs to the technical field of genetic engineering and bioengineering. A delta-tocotrienol metabolic pathway is constructed in Yarrowia lipolytica, a mutant of a key enzyme, benzoate phytyltransferase, is screened, and the production of delta-tocotrienol is further improved by strengthening the shikimic acid pathway. The production of delta-tocotrienol of the constructed engineering strain can reach 466.8 mg / L, which lays a foundation for subsequent biosynthesis of vitamin E compounds, and has potential value and significance for the development of synthetic biology.

The present application relates to: a microorganism of the genus Yarrowia having a retinoid-producing ability, in which the activity of a PHO84 protein is increased compared to the endogenous activity thereof; a retinoid production method using the same; a method for producing a microorganism of the genus Yarrowia having a retinoid-producing ability; and a composition for producing a retinoid.

The invention belongs to the technical field of bioengineering, and particularly relates to a lipid-decomposing yeast engineering bacterium for producing DHA (docosahexaenoic acid) as well as a construction method and application thereof. The lipolytic yeast engineering bacterium is obtained by taking lipolytic yeast as a chassis strain, optimizing T-pfaA, T-pfaB, T-pfaC and T-pfaE genes through codons and then performing heterologous expression in the lipolytic yeast. The method comprises the following steps: transferring a transformed strain seed solution into an SD-Leu C / N 80 nitrogen-limited fermentation culture medium, collecting thalli after fermentation, carrying out freeze-drying, carrying out fatty acid detection, and carrying out quantitative analysis on DHA by adopting GC-MS (Gas Chromatography-Mass Spectrometer) to obtain a DHA standard curve: y = 1.39 * 10 <-8 > x <-0.0007 >, R2 = 0.9991, x is the peak area, y is the DHA yield, the unit is g / L, the DHA yield of the transformed strain is 0.19 mg / g, and a basis is provided for industrial directional synthesis of DHA.