41 results about "Yarrowia" patented technology

The invention discloses a Yarrowia lipolytica strain for synthesizing ergothioneine and application of the Yarrowia lipolytica strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a key enzyme TrEGT1 is subjected to semi-rational transformation, so that the ergothioneine yield of a strain expressing a mutant is increased by 240.9%, precursor metabolic network screening is further modularized, precursor flux is enhanced, a competitive approach of a precursor is knocked out and weakened, and strategies such as fermentation process optimization and the like are further optimized; and when the constructed strain is fermented in a 5L bioreactor, the yield of ergothioneine can reach 10.3 g / L. The highest-level ergothioneine synthesis reported at present is realized, the feasibility of synthesizing the ergothioneine by using the Yarrowia lipolytica as the chassis cell is verified, and a reference value is provided for efficiently synthesizing the ergothioneine by using other chassis cells.

The invention provides a genetically engineered bacterium for producing astaxanthin, and the genetically engineered bacterium takes Yarrowia lipolytica Po1f as an original strain and contains a GGS gene, an HMGR gene, a beta-carotene synthesis gene, a beta-carotene ketolase gene and a beta-carotene hydroxylase gene. The invention further provides the Yarrowia lipolytica MC247, and the preservation number of the Yarrowia lipolytica MC247 is CGMCC NO.29699. According to the Yarrowia lipolytica MC247, the high-yield astaxanthin is obtained, a new technical scheme is provided for efficient and green manufacturing of the astaxanthin, and industrial production of the astaxanthin is facilitated.

The invention relates to a hot extraction formula of wild ancient tree black tea and coffee. The hot extraction formula is prepared from the following raw materials by weight: 3-5g of Yunnan wild ancient tree black tea; 300 to 500g of mountain spring water; 8-12 g of coffee beans; 20-35 g of an espresso coffee liquid; 1-3 g of crushed fresh orange peel; 1-2g of green prickleyash; 1-2g of cinnamon sticks; and 50-60g of iced litchi honey water. According to the hot extraction formula of the wild ancient tree black tea and the coffee, the coffee retains jasmine flower fragrance and citrus acid of Yarrowia and pungent fragrance of green prickleyash through hot extraction, the bitter taste is weakened through cold extraction of the black tea, the flower fragrance is prevented from being damaged by high temperature through cold extraction of the black tea, and the sweetness of cinnamon and honey of lychee flowers correspond to the honey note of the ancient tree tea, the active acidity of the hot coffee, the mild and sweet taste of cold tea soup and the slight numb stimulation of the green prickleyash; the wooden sweet fragrance of cinnamon releases honey charm of ancient tree black tea and tartaric acid of coffee through low-temperature slow extraction, the complexity is improved by the aid of oriental spices, the drinking experience of cold and hot overlapping is created, memory points are made through spice intervention and temperature contrast, and the method is suitable for being put out as a season-limited style in a high-quality caffeine.

The invention discloses a method for improving yield of progesterone in yarrowia lipolytica, which comprises the following steps: (1) constructing a biosensor responding to steroid hormone, and the nucleotide sequence of the sensor is as shown in SEQ ID No. 52; (2) a biosensor responding to steroid hormones is connected with a lipid synthesis key gene DGA1 in yarrowia lipolytica and spliced with a linearized pYRSB carrier, a recombinant plasmid pDTY316 is obtained, and the nucleotide sequence of the gene DGA1 is shown as SEQ ID No.57; the nucleotide sequence of the plasmid pYRSB is as shown in SEQ ID No. 54; (3) transforming the recombinant plasmid pDTY316 into a recombinant strain yDTY314 to obtain a recombinant strain yDTY316; and (4) carrying out shake flask fermentation on the recombinant strain yDTY316 to realize high yield of progesterone. Experiments prove that the yield of the progesterone obtained by the method is high.

The invention discloses saccharomycetes G-1 and application thereof in degradation of gibberellin GA3, the Latin name of the saccharomycetes G-1 is Yarrowia lipolytica (Yarrowia lipolytica), the strain of the saccharomycetes G-1 is preserved in the China General Microbiological Culture Collection Center on December 1, 2024, and the preservation number is CGMCC No.32800. The invention further discloses a preparation method of the saccharomycetes G-1. The yeast G-1 is screened from a gibberellic acid fermentation filter residue organic fertilizer sample, the strain can grow in an inorganic salt culture medium with gibberellin GA3 as a unique carbon source, and the degradation efficiency of GA3 reaches 24% after the strain is cultured for 48 h; the microbial agent prepared from the microbial agent is applied to the composting process of fermentation filter residues, and the result shows that degradation of GA3 is obviously accelerated by adding 3% of the microbial agent to a treatment group. The strain has great application potential in the aspect of promoting harmless treatment of fermentation filter residues. The invention provides a new way for removing residual plant hormone gibberellin GA3 in soil and organic fertilizers, enriches a germplasm resource library of plant hormone degrading bacteria, and has a better application prospect.

The present invention relates to mutants of the bumblebee Yarrowia SBLE enzyme having modulated transesterification and / or esterification activity compared to the bumblebee Yarrowia SBLE enzyme, and to homologues of the bumblebee Yarrowia SBLE enzyme, such as the C. bogoriensis LE enzyme, the B. batistae LE enzyme and the R. riograndensis LE enzyme, having transesterification and esterification activity on bolaform glycolipids. The present invention also provides genetically modified yeast strains comprising a mutant LE enzyme or a bumblebee Yarrowia SBLE enzyme homologue of the present invention. The present invention further provides the use and methods of the mutant LE enzymes, the bumblebee Yarrowia SBLE enzyme homologues and the modified yeast strains of the present invention for the production of acidic glycolipids, lactone-form glycolipids and / or glycolipid oligomers / polymers / esters. The present invention also provides the three-dimensional structure of the bumblebee Yarrowia SBLE enzyme and its use for the design of mutants having modulated hydrolysis and / or transesterification activity.

The present application relates to a kind of coal slime collector preparation method using kitchen waste oil, comprising the kitchen waste oil after being pretreated with water, emulsifier is mixed, as carbon source configuration first culture medium, Bacillus and Rhodococcus species are inoculated in it and carry out aerobic fermentation, obtain refined oil, and as carbon source configuration second culture medium, Candida species are inoculated in it and carry out aerobic fermentation, obtain mixed fatty acid, and as carbon source configuration third culture medium, Yarrowia species are inoculated in it and carry out micro-aerobic fermentation, obtain alkane mixture;Emulsified oil and / or refined oil are as carbon source configuration fourth culture medium, Pseudomonas species are inoculated in it and carry out aerobic fermentation, obtain rhamnolipid;Alkane mixture is mixed with rhamnolipid, obtain coal slime collector.The present application develops a new type of green, good coal slime collector by constructing multistage series biological transformation process.

The application discloses a process for producing resveratrol by fermentation of Yarrowia lipolytica, and belongs to the technical field of microorganisms. Through a series of fermentation condition optimization, including adjustment of the basic medium composition, concentration, feeding composition, pH and feeding mode. The fermentation yield after adjustment is increased from 6g / L at the beginning to 22g / L, and the purity of the extracted resveratrol is greater than 80%, which solves the problems of insufficient yield, difficult extraction and low purity compared with resveratrol from plants.

The invention relates to the technical field of genetic engineering and bioengineering, in particular to a Rhodococcus lipolytica engineering bacterium and application thereof in preparation of sclareol, the Rhodococcus lipolytica engineering bacterium comprises SsLPPS, SsSCS, nucleotide for encoding polypeptide interaction tags RIAD and RIDD, and the nucleotide for encoding polypeptide interaction tags RIAD and RIDD is used for encoding polypeptide interaction tags RIAD and RIDD. The Yarrowia lipolytica engineering bacterium overexpresses an MVA pathway key gene and a GGPP synthesis related gene, and the MVA pathway key gene is selected from erg8, erg10 and erg20; the GGPP synthesis related gene is selected from tPaGGPPS and / or SsGGPPS, and the GGPP synthesis related gene is selected from the group consisting of tPaGGPPS and / or The promoter of the erg9 gene of the Yarrowia lipolytica engineering bacterium is a truncated promoter, and the length of the promoter is 40-110 bp. According to the method, the sclareol is efficiently synthesized in the Yarrowia lipolytica, and the final shake flask yield reaches 1.5 g / L.

Yarrowia lipolytica Yl590 and the use thereof. The Yarrowia lipolytica Yl590, taxonomically classified as Yarrowia lipolytica, was deposited with the China General Microbiological Culture Collection Center on 4 June 2024, with a deposit address of Beijing, China and a deposit number of CGMCC No. 30855. The present invention can solve the problem of the slow growth rate of Yarrowia lipolytica in the prior art, and is suitable for the technical field of microorganisms.

The invention relates to a process for preparing alpha-ketoglutaric acid based on citrus fermentation liquor, which comprises the following steps: inoculating AKG-producing arrowia lipolytica seed solution into the prepared citrus fermentation liquor, regulating and controlling the dissolved oxygen concentration and pH value in stages in a fermentation period, and adding a fermentation regulator, thereby obtaining the alpha-ketoglutaric acid. AKG fermentation liquor is sequentially subjected to centrifugation, membrane filtration and continuous chromatographic separation and purification, AKG seed crystals are added for induced crystallization to obtain an AKG finished product, a metabolic network of the arrowia lipolytica is accurately regulated and controlled in a dynamic fermentation mode of dissolved oxygen step control, pH stage control and fermentation regulator supplementing, the concentration of the AKG in the fermentation liquor is increased, and the yield of the AKG is increased. The method provided by the invention has the advantages of simple process and low cost, by-product accumulation is reduced, a multi-step synergistic separation and purification mode of pretreatment, membrane filtration and continuous chromatographic separation in the separation and purification stage is matched, fermentation products can be efficiently recovered and purified, and the separation yield and the purity of an AKG finished product are improved, so that efficient and green preparation of AKG is realized, and the method has extremely high industrial application value.

The present application relates to a method for producing retinol comprising a step of culturing a Yarrowia microorganism in a medium containing an antioxidant, a method for increasing retinol production, a method for producing a retinoid, a medium composition for a Yarrowia microorganism for producing retinol containing an antioxidant, and a composition for producing retinol comprising the microorganism or a culture thereof and an antioxidant.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing nervonic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and biological engineering. Beta-keto acyl-CoA synthase AtKCS, CraKCS and LaKCS are expressed in the Yarrowia lipolytica, recombinant Yarrowia lipolytica capable of synthesizing nervonic acid is obtained, supply of C18: 1 delta9-CoA is further enhanced, accumulation of C24: 0-CoA is reduced, endoplasmic reticulum is expanded, key genes are driven by a strong promoter, supply of malonyl CoA and NADPH is increased, and the yield of nervonic acid is increased, the nervonic acid yield of the engineering strain NA10 reaches 451.80 mg / L, and a foundation is laid for subsequent biosynthesis of lipid derivatives such as C24: 1-ceramide and the like.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

The invention relates to the technical field of bioengineering, in particular to a method for synthesizing long-chain fatty acid and ultra-long-chain fatty acid through biotransformation of carbon dioxide. According to the present invention, the long-chain fatty acid and the ultra-long-chain fatty acid are produced by using the clostridium endophyllum and the Yarrowia lipolytica through the two-step bioconversion cascade carbon sequestration method and using the carbon dioxide as the carbon source through the fermentation, and the long-chain fatty acid and the ultra-long-chain fatty acid are produced, wherein the long-chain fatty acid and the ultra-long-chain fatty acid are used as the carbon source, such that the long-chain fatty acid and the ultra-long-chain fatty acid are produced, according to the method disclosed by the invention, the dependence of the traditional microorganism synthesis of long-chain fatty acid and ultra-long-chain fatty acid on a carbohydrate carbon source is eliminated, the bioconversion from carbon dioxide to the long-chain fatty acid and the ultra-long-chain fatty acid is realized, and a new carbon source selection and method are provided for the synthesis of the long-chain fatty acid and the ultra-long-chain fatty acid.

The present application relates to a Yarrowia microorganism that expresses Dunaliella salina-derived geranylgeranyl pyrophosphate synthase and has the ability to produce carotenoids or substances whose precursors are carotenoids, a method for producing carotenoids or substances whose precursors are carotenoids using the same, a composition for producing carotenoids or substances whose precursors are carotenoids, and use of the Yarrowia microorganism or a culture thereof for producing carotenoids or substances whose precursors are carotenoids.

This invention discloses a high-yielding erythritol-producing *Yarrowia lipophila* strain (… Yarrowia lipolytica This invention relates to the field of biotechnology, specifically to *Yarrowia lipophila* CA20 as the starting strain, and its applications. A000039 A gene knockout method was used to construct a high-yield erythritol-producing *Yarrowia lipolytica* strain. The construction method provided by this invention is simple and of significant value in simplifying the construction process of high-yield strains. Furthermore, under the fermentation medium and conditions provided by this invention, the *Yarrowia lipolytica* strain producing high-yield erythritol was fed-batch fermented with glucose as a substrate, achieving a yield of 280.50 g / L and a yield of 0.71 g / g, demonstrating good economic benefits.

The present application relates to a Yarrowia microorganism that expresses geranylgeranyl pyrophosphate synthase derived from Haematococcus pluvialis and has the ability to produce a carotenoid or a substance whose precursor is a carotenoid, a method for producing a carotenoid or a substance whose precursor is a carotenoid using the same, a composition for producing a carotenoid or a substance whose precursor is a carotenoid, and use of the Yarrowia microorganism or a culture thereof for producing a carotenoid or a substance whose precursor is a carotenoid.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing abscisic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, heterologous synthesis of ABA is realized in the Yarrowia lipolytica, precursor supply is optimized by enhancing carbon flow in a metabolic pathway, the activity of key enzymes BcABA2 and BcABA3 is improved by adopting a semi-rational strategy, NADPH regeneration engineering is implemented to enhance the oxygen uptake ability of a strain, a cytoplasm-peroxisome engineering system is constructed, and the oxygen uptake ability of the strain is enhanced. The ABA synthesis is further enhanced by utilizing the characteristic of high-yield acetyl coenzyme A of peroxisome. According to the invention, fermentation conditions are further optimized, so that the ABA accumulation concentration of the constructed engineering strain in a 5 L bioreactor reaches 2554.36 mg / L, and the highest titer of ABA biosynthesis reported so far is created. The metabolic modification strategy provides important reference for biosynthesis of other terpenoids.

The present invention discloses flavonoid 4'-O-methyltransferase and its application in the production of hesperetin, belonging to the field of genetic engineering and bioengineering technology. The present invention screened and identified new flavonoid 4'-O-methyltransferases derived from tea-branch orange and Huazhou pomelo; the flavonoid 4'-O-methyltransferases CrcOMT-2 and CgtOMT-3 obtained by screening were integrated and expressed in the multi-copy site rDNA and ZETA site on the genome of Yarrowia lipolytica YE26, respectively, to achieve the de novo synthesis of hesperetin. The Yarrowia lipolytica Z02 constructed by the present invention achieved the de novo synthesis of 130.2 mg / L hesperetin in a 5L fermentor, which is also the highest yield of hesperetin de novo synthesis in microorganisms reported, laying the foundation for the subsequent biosynthesis of flavonoids.

The present application relates to the preparation process of the lysate product of bifidobacterium fermentation honey and extract of yarrow, comprising the preparation of bifidobacterium activation medium; strain liquid propagation; composition preparation; anaerobic fermentation culture; finally, the fermentation broth of the above composition is inactivated by low-temperature micro-jet, centrifuged, and the supernatant is obtained, and the lysate product of bifidobacterium is obtained. Compared with the blank culture medium, the total number of viable bacteria and OD value can be obviously improved by adding glucose or honey as carbon source, and when the carbon source is further selected as honey, the total number of viable bacteria after fermentation culture by the culture medium is more, and the OD value is higher.

The invention belongs to the technical field of natural product extraction, and particularly relates to a method for extracting and purifying astaxanthin from yarrowia lipolytica fermentation liquor. The method comprises the following steps: firstly, carrying out engineering transformation on yarrowia lipolytica to obtain yarrowia lipolytica engineering bacteria capable of producing astaxanthin, culturing and fermenting the engineering bacteria to obtain astaxanthin, and then carrying out wall breaking on yeast fermentation liquor containing astaxanthin by utilizing a high-pressure homogenization method, so that astaxanthin components in the yeast fermentation liquor are fully dissolved out, and the yield of the astaxanthin is improved. And extracting and separating by adopting an organic solvent, and purifying the obtained crude astaxanthin extracting solution by using macroporous adsorption resin to finally obtain the astaxanthin with high yield and high purity. By adopting the method disclosed by the invention, not only can the astaxanthin with higher content be obtained, but also the purity of the astaxanthin is 99.2% or above, and the method is very suitable for large-scale production of the astaxanthin and has very good economic benefits.

This invention belongs to the field of vinegar fermentation and processing technology, specifically relating to a medicinal and edible vinegar for improving postprandial blood sugar and its segmented fermentation method. The medicinal and edible vinegar for improving postprandial blood sugar is made from highland barley and a compound powder of medicinal and edible ingredients. The segmented fermentation method includes: adding brewing yeast to highland barley and fermenting to obtain fermentation mash; adding the compound powder of medicinal and edible ingredients to the fermentation mash and performing coupled fermentation; subsequently inoculating with acetic acid bacteria for acetic acid fermentation; and adding functional strains such as *Yarrowia spp.* for targeted metabolism of residual sugars in the middle stage; finally, after post-treatment, the medicinal and edible vinegar for improving postprandial blood sugar is obtained. The segmented fermentation method of this invention can significantly improve the retention rate of active ingredients and fermentation stability, giving the resulting medicinal and edible vinegar for improving postprandial blood sugar the advantages of natural low sugar, mellow flavor, and high retention rate of active ingredients. It is suitable for the industrial production of low-sugar, highly stable medicinal and edible health vinegar.

The present application relates to: a Yarrowia genus microorganism having a retinol-producing ability, in which the activity of an ABC3 protein is reduced as compared to the endogenous activity thereof; a retinol production method using the same; a method for producing a microorganism of the genus Yarrowia having a retinol-producing ability; the invention discloses a method for enhancing retinol release of microorganisms. And a composition for producing retinol.

The invention relates to the technical field of plant disease prevention and control, in particular to a composition containing mortierella, oleaginous yeast and streptomyces as well as a preparation and application of the composition. The composition comprises strains of Mortierella alpina (Mortierella alpina) B28-1, strains of oil-producing yeast (Saitozymapoda) 85151 and strains of streptomyces aureomycin (Streptomyces aureomycin) 90372, conidia and / or volatile matters of bacterial cultures, and the volume ratio of the Mortierella alpina B28-1 to the oil-producing yeast 85151 to the streptomyces aureomycin 90372 is 1: 1: 1. The composition and the preparation thereof are used for biological prevention and treatment of pseudo-ginseng diseases, relieving the soil continuous cropping effect in the pseudo-ginseng growth process and promoting pseudo-ginseng growth. The method has a wide application prospect.

The application discloses a Yarrowia lipolytica, a preparation, application and a preparation method of erythritol, and belongs to the technical field of microorganisms. Yarrowia lipolytica The Yarrowia lipolytica is preserved in the China General Microbiological Culture Collection Center on December 26, 2022, and the preservation number is CGMCC No. 26283. The Yarrowia lipolytica Yarrowia lipolytica The Yarrowia lipolytica has simple culture conditions, high temperature resistance (35 DEG C), a glucose-to-erythritol conversion rate of 71%, high erythritol yield, few by-products, high yield, easy separation and purification of products, and can effectively reduce the production cost of erythritol in industrial production.

The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to a method for biologically synthesizing SNAC, which comprises the following steps: inoculating yeast to a fermentation medium, adding 2-hydroxy-N-octyl benzamide and a salt-forming agent, controlling the pH value of the fermentation medium to be alkaline, and carrying out biological fermentation to synthesize the SNAC, wherein the yeast is selected from one of candida tropicalis, candida maltose and yarrowia lipolytica. According to the method, a biological method and a chemical method are organically fused, respective advantages of the biological method and the chemical method are fully played, a brand new SNAC synthesis route mainly depending on microbial fermentation is innovatively developed, green synthesis of the SNAC is realized by utilizing a microbial cell factory, the synthesis route of the SNAC is greatly shortened, and the method has the advantages of cheap and easily available raw materials, lower cost, mild reaction conditions, high yield and the like. And the method has the advantages of low environmental pollution, high atom economy, green production and the like.

The present invention relates to a process for fermenting Yarrowia in a bioreactor, comprising cultivating Yarrowia in a medium, and adding a feed comprising formic acid and a sugar to the medium in a molar ratio of formic acid to sugar of 1 to 13 mol formic acid / mol monosaccharide.

The invention discloses Yarrowia lipolytica, a preparation, application and a preparation method of erythritol, and belongs to the technical field of microorganisms. The invention provides Yarrowia lipolytica which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on December 26, 2022, and the preservation number of the Yarrowia lipolytica is CGMCC (China General Microbiological Culture Collection Center) No.26283. The Yarrowia lipolytica is simple in culture condition and resistant to high temperature (35 DEG C), the conversion rate of erythritol synthesized from glucose reaches 71%, the yield of erythritol is high, byproducts are few, the yield is high, the product is easy to separate and purify, and the production cost of erythritol can be effectively reduced when the Yarrowia lipolytica is applied to industrial production.