780 results about "Continuous fermentation" patented technology

One embodiment of the present invention is method for making an alcohol concentrate using a nested batch fermentation process, wherein multiple rounds of wort fermentation, distillation and refortification, is described. The rounds are repeated until the desired concentration of fermented wort is obtained. Another embodiment of the present invention is a method for a continuous fermentation process, wherein portions of the fermentation are distilled and the remaining wort is returned to the active fermentation. This process is repeated until the desired concentration of the fermented wort is obtained. Yet another embodiment of the present invention is a method for extraction of hops compounds, wherein the distilled alcohol from the nested or continuous fermentation process is used.

The invention discloses a production method for a biological organic fertilizer which belongs to the field of manufacturing of composite fertilizers. The production method comprises the following steps: with pig manure, cow dung, chicken manure, sheep manure, vinasse, vinegar residue, cassava residue, furfural residue, uncontaminated river mud, corn straw, peanut straw, rape straw, a cottonseed cake, plant ash, a deodorant, a biological nitrogen fixation bacterium, a phosphate solubilizing bacterium, a potassium solubilizing bacterium, a nodule bacterium and a phosphorus activator used as raw materials, carrying out tank continuous fermentation to allow the raw materials become thoroughly decomposed; carrying out deodorizing and removing water; and carrying out granulation to obtain the granular biological organic fertilizer. The fertilizer has the following advantages: a complete range of nutrient elements, capacity of improving physical and chemical properties of soil and enhancing water retention, fertilizer retention and fertility supplying capacity of the soil, no corrosion and damage to roots and seedlings and capacity of substantially improving the quality of an agricultural product.

A method for regulating fermentation of organic material, such as cocoa beans and pulp is disclosed. The method includes adding at least one strain of lactic acid bacteria and/or acetic acid bacteria to the organic material. Optionally, at least one strain of yeast is added. Bacterial strains and compositions, useful in the described method, are also disclosed. The use of the described starter cultures and compositions permits faster fermentation, fermentations with targeted population dynamics and succession of microorganisms, and fermentations with targeted levels of both desirable and undesirable metabolites.

The invention relates to a stationary type organic fertilizer fermentation machine. The stationary type organic fertilizer fermentation machine comprises a substrate, a fermenter, a motor, an electric cabinet, an air purifier and microwave electromagnetic heaters; the two ends of the fermenter are fixedly erected on the substrate; the motor is electrically connected with the electric cabinet; a rotating shaft is arranged on the motor; the rotating shaft is a hollow pipeline and is provided with an air outlet; the rotating shaft is penetrated through the middle part of the fermenter; a plurality of blades which are forwards slantwise are extended on the rotating shaft; the motor drives the rotating shaft to rotate so that the blades fixed on the rotating shaft drive organic materials to synchronously rotate along with the blades and push the organic materials forward from the front end to the back end; the air purifier is positioned at the upper part of the back end of the fermenter; and the two microwave electromagnetic heaters are respectively arranged at the two ends of the bottom of the outer wall of the fermenter. The stationary type organic fertilizer fermentation machine realizes the continuous fermentation of organic fertilizers, is high-efficiency and energy-saving, has strong practical applicability, strong adaptability and more complete functions and can be matched with the traditional bacterial agents. The stationary type organic fertilizer fermentation machine can adapt to multiple raw materials and the mixture of raw materials with different moisture contents as well as multiple climatic environments.

A method for preparing biogas by joint anaerobic fermentation of food waste, urban sludge and urban excrement, comprising the following steps: (1) pretreatment system: food waste, urban sludge and urban excrement undergo a pretreatment process to obtain granules (2) homogeneous system: the materials processed in step (1) enter the homogenizing tank one after another, mix evenly and degrade part of the suspended matter, so that the particle size of the solid material is less than 20-25mm, and obtain Fermentation substrate with a solid content rate of 8 to 12%; (3) anaerobic fermentation system: the fermentation substrate treated in step (2) is transported to an anaerobic fermentation tank by a pump and adopts a continuous fermentation process and a mesophilic anaerobic fermentation The anaerobic fermentation method combined with jet stirring produces biogas, biogas residue and biogas slurry. The combined anaerobic fermentation preparation method of the present invention has the effects of complete material fermentation and high gas production rate.

The invention discloses a process for preparing succinic acid through coupling fermentation and membrane separation units, the process for preparation comprises following steps: firstly, inoculating fermentation seeds of bacteria strains which produce the succinic acid into a fermentation tank to culture, then, coupling microfiltration or ultrafiltration membrane separation units with the fermentation tank to realize semi-continuously or continuously preparing the succinic acid with fermentation, separating out part of fermentation liquor, releasing from the control of metabolites, returning somatic cells and components which are cut off by the membrane separation units back into the fermentation tank and continuingly fermenting, continuingly using substrate which is not consumed in the fermentation liquor which is nanofiltrated, reclaimed, and separated to ferment, and simultaneously adding the fermentation culture medium to keep the volume of the fermentation liquor in the fermentation tank constant. The invention not only can release from the control of the metabolites and increase the production strength of the succinic acid, but also uses reducing sugar which is not consumed in the liquor which is nanofiltrated, reclaimed, and separated, simultaneously concentration is available, the technical condition is moderate, environmental pollution is little, which is easy to control automatically and has wide social and economic benefits.

An improved method and device is accomplished for ethanol production using an in-line extraction of ethanol by ultrasonic atomization, thereby removing the effect of the ethanol inhibition factor that adversely affects the rate and yield. The in-line removal of ethanol as it is formed increases the fermentation rate, improves the yield and uses 20-25% of the energy required as compared to thermal distillation processes currently used. The improved method makes sure that ultrasonic vibration does not deactivate enzymes to any significant level. The elimination of the effect of the ethanol inhibition factor allows for a continuous fermentation process as opposed to the costly, time-consuming repeated batch processes.

The invention discloses a fermentation production method of sialic acid and belongs to the technical field of bioengineering. The method comprises the following steps: adding a seed liquid into a fermentation tank containing a sterilizing fermentation culture medium; continuously fermenting to produce fermentation liquid containing polysialic acid in a way of replenishing feeding liquid and a hydrogen dioxide solution; then carrying out centrifugal separation, ethanol precipitation and filtration to obtain refined polysialic acid; then, carrying out acidolysis and crystallization on the polysialic acid; and finally, washing and freeze-drying to obtain sialic acid powder. The output of the polysialic acid produced by the method provided by the invention can reach 12.96g/L, the already reported maximum content of the polysialic acid is improved by 87.28%, the hydrolysis rate of polysialic acid is 95%, the content of purified sialic acid reaches 10.01g/L and the purity reaches 94.80%. The method is suitable for industrial promotion and application.

The invention belongs to the technical field of producing biological butanol by fermentation, which relates to a method and a device for producing the biological butanol by extracting and fermenting starch substances and sugar substances. The method comprises the following steps: discharging fermented liquor from a last fermentation tank; then feeding the fermented liquor into a next fermentation tank for continuous fermentation; adding an extracting agent to be mixed for extracting butanol in the bulking tank and conveying process of feeding the fermented liquor from the last fermentation tank to the next fermentation tank; extracting and separating the butanol generated by the fermented liquor with the extracting agent; and feeding the fermented liquor separated from a mixture of the extracting agent and the butanol into the next fermentation tank for fermentation. The invention realizes the method and the device for producing the biological butanol by the continuous extraction and fermentation. The biological butanol can be produced by a method for continuously adding the extracting agent, continuously separating the biological butanol and continuously supplementing original fermented liquor. The invention can improve the yield of the biological butanol by 3 times to maximum extent, save one second of steam, reduce the discharge of over 60 percent of wastewater and bring considerable economic benefits to enterprises for producing the biological butanol.

The invention discloses a method for producing butane diacid through continuous fermentation or semi-continuous fermentation, which belongs to the bioengineering technical field. The invention provides application of Actinobacillus succinogenes in the method for continuously or semi-continuously preparing the butane diacid by utilizing carbohydrate raw materials such as cane molasses, corn starch syrup, Jerusalem artichoke hydrolysis syrup, sweet sorghum straw syrup, and lignocellulose hydrolysis syrup and so on. The method utilizes multi-step continuous fermentation or two-step semi-continuous fermentation, which can improve germ concentration and cell activity and can obtain high butane diacid output and high butane diacid production intensity; the method is easy to realize automatic and continuous operation; compared with batch fermentation, the method can save non-fermentation time such as repeated tank cleaning, sterilization and so on, so the production efficiency can be greatly improved; the semi-continuous fermentation for producing the butane diacid is easier to control fermentation parameters than the continuous fermentation, has high sugar utilization rate, target product yield and target product output, has simple and easy equipment and operation, and is suitable for industrialized production.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention discloses a preparation method of kiwi fruit enzyme with high anti-oxidation activity. A formula is characterized in that fresh kiwi fruits are adopted as raw materials, fruit skin is reserved for pulping after washing and deburring the kiwi fruits, and enzymolysis is carried out on kiwi fruit pulp by adding a composite enzyme preparation (which is prepared from pectinase, cellulose, hemicellulase, protease and diastase), so that the juice yield and the production efficiency are improved; then, a multi-strain symbiotic fermentation technology and a grading inoculation process are adopted, and fermentation and ageing are carried out on a product by sequentially adding saccharomycetes, acetic acid bacteria and lactic acid bacteria; finally, the purpose of removing bacteria is achieved through the technologies of kieselguhr filter, membrane filter and the like, so that continuous fermentation of the product is ended, the stability of the shelf life is ensured, and meanwhile, the activity of active ingredients and enzyme is maximum kept. The enzyme prepared through the preparation method has the characteristics of high anti-oxidation activity, richness in enzymes and high enzyme activity; the appearance is clear and transparent, the flavor is sour, sweet and gentle, and the fruity fragrance is elegant.

The invention discloses a method for continuously producing biological butanol by fermentation, separation and coupling of a static bed. The method comprises the following steps of: filling a modified immobilized medium into the static bed, and circularly connecting in series with a bioreactor; incubating a butanol producing strain into the bioreactor, culturing the butanol producing strain in a fermentation medium, circulating fermentation liquor between the static bed and the bioreactor, and gradually absorbing free thallus cells into the static bed; pumping the fermentation liquor into a pervaporation membrane module for separation when the concentration of the butanol in the bioreactor is between 3 and 7g/L, collecting penetrating fluid, and returning liquid intercepted by a membrane to the bioreactor for continuous use; meanwhile, adding the fermentation medium into the midstream of the bioreactor, and continuously fermenting to produce the butanol. The method obviously weakens the pollution of the pervaporation membrane existing in the process of in situ separation and coupling, prolongs the operation time and service life of the membrane, eliminates butanol product inhibition in a fermentation process, and realizes the high-efficient and continuous production of the biological butanol.

The invention relates to a method for preparing flammulina velutipes polysaccharide with simple operation and low equipment investment, which comprises the following process procedures: activating a slant strain, shaking culture of seeds, submerged fermentation in liquid, continuous fermentation, separation by an ultrafiltration membrane, condensation and alcohol precipitation. The method for preparing the flammulina velutipes polysaccharide has the advantages of continuous fermentation in a fermenter, prolonging the fermentation time, simplifying the operation flow and improving the polysaccharide yield.

The invention relates to a process and a system for producing ethanol through solid-state continuous fermentation and distillation of sorgo straws, wherein the process comprises a raw material pulverizing and preprocessing step, a strain adding step, a solid-state fermentation step and a solid-state distillation step; the solid-state fermentation step is continuously finished in a multilayer multisection solid-state continuous fermentation device, and the solid-state distillation step is continuously finished in a multilayer differential pressure type solid-state continuous distillation device, thereby radically overcoming the abuse that the past solid-state fermentation and distillation processes can not continuously produce, realizing continuous, mass and automated production and processing, greatly improving the production efficiency of preparing the fuel ethanol from the sorgo straws, and achieving the purposes for lowering the production cost, improving the yield and improving the economic benefit; an adopted continuous production system device is particularly suitable for industrialized large-scale production, thereby promoting the generalization and the application of a biological energy source technology.

The invention relates to a production method of mushroom dreg and cow dung composite organic fertilizer, which effectively achieves production of the organic fertilizer with mushroom dreg and cow dung. The method comprises the steps of selecting the mushroom dreg after edible mushroom cultivation in the edible mushroom industry, grinding into mushroom dreg particles after bag removal and airing, mixing the mushroom dreg particles with the cow dung as a fermentation and compost inoculant, adding an organic fertilizer fermentation inoculant, uniformly stirring, adding water to prepare into a fermentation material, establishing the fermentation material into a pile, covering with a plastic film for fermentation for 24-72h, carrying out pile turning for the first time when the temperature rises to 40 DEG C, then spraying water, stirring uniformly, establishing a pile again, covering with the plastic film for continuous fermentation, carrying out fermentation for 24-36h after the temperature of the pile raises to 55-65 DEG C, then carrying out the pile turning, and carrying out the pile turning every other 2-3 days till the temperature of the pile does not rise. The method is simple, facilitates production and preparation, has a good application effect, achieves full use of waste, can effectively obtain the organic fertilizer for crops, improves soil, increases output and does not have environmental pollution, and raw materials are rich.

The invention discloses an active organic bacterial manure and a preparation method thereof. The active organic bacterial manure is prepared by the following steps of: performing one-time continuous fermentation, granulation, drying and screening on plant ash, industrial and agricultural wastes enriched with organic matters, and an inorganic fertilizer which are used as raw materials and inoculating bacteria subjected to breeding from the raw materials above. According to the active organic bacterial manure, the industrial and agricultural wastes enriched with the organic matters can be greatly consumed, and the environmental pollution is reduced; in the process of crop cultivation and urban greening, the problems of soil hardening, gradual fertilizer efficiency reduction and environmental pollution which are caused by that fertilizers are used year by year are solved; and a new fertilizer source is supplied to the agricultural production.

The invention provides a method for production of ethanol by continuous fermentation of immobilized yeast cells, and the method comprises the following steps: (1) a fiber material is attached to a support frame, and is filled into a bioreactor; (2) yeast cells are immobilized in the fiber material in the bioreactor; (3) a fermentation medium is added into the bioreactor to begin ethanol fermentation; (4) when glucose in the fermentation medium is consumed, a fermentation liquid flows out of the bioreactor, the ethanol is collected, the fermentation medium is added, and then the step (4) is repeated. The method uses the fiber material for immobilization of the yeast cells for continuous fermentation to produce the ethanol, the fiber material as an immobilization material is stably in property, non-toxic, and good in adsorption and mass transfer effects, can be repeatedly used, is easy to achieve continuous production of the ethanol, and in a continuous fermentation process can greatly improve the ethanol yield.

The invention discloses a high-yield arginine deiminase bacterial strain and a method for producing L-citrulline by the same. The high-yield arginine deiminase bacterial strain of the invention is streptococcus faecalis BM-2 CGMCC No.4990. After small-scale fermentation of the bacterial strain in a 30-L fermentation tank, the L-citrulline yield is up to 98 g/L, which is increased by 50% when compared with the L-citrulline yield of the original strain; after continuous fermentation for 5 batches, the L-citrulline yield of the bacterial strain is stable, and the L-citrulline yield level is obviously higher than that of reported bacterial strains; and the bacterial strain of the invention has important industrial production application value.

The invention discloses a method for producing pyrroloquinoline quinine through microbial fermentation and a fermentation medium used in the method for producing the pyrroloquinoline quinine through the microbial fermentation. An oxygen control fermentation culture method is used for culturing methylotrophic bacteria, a carbon source contained in the fermentation medium is methyl alcohol, and a nitrogen source contained in the fermentation medium is ammonium sulfate, a dissolved oxygen level is controlled by stages to be 30%-50% of saturated dissolved oxygen in the fermentation process, pH is controlled to be 5.5-6.0 through fed-batch, the fed-batch is achieved through a pH-nitrogen source joint control technology, the methylotrophic bacteria are cultured for six days in a 7.5L fermentation tank, and eventually the cell density (OD 600) exceeds 10, yield of PQQ reaches 2g/L, and production intensity of the PQQ is 333.33mg/L/day. The method for producing the pyrroloquinoline quinine through the microbial fermentation is simple in operation, automatic control and continuous fermentation can be achieved easily, and a solid foundation for industrially producing the pyrroloquinoline quinine through the methylotrophic bacteria is laid.

The invention discloses a production method for a biological organic fertilizer containing medium trace element chelates of humic acid. The biological organic fertilizer belongs to the field of manufacturing of composite fertilizers. The production method comprises the following steps: with pig manure, human feces, cow dung, rabbit manure, vinasse, cassava residue, leftovers in production of monosodium glutamate, trench mud, corn straw, soybean straw, vetch straw, a rapeseed cake, a tea seed cake, a deodorant, a biological nitrogen fixation bacterium, a phosphate solubilizing bacterium, a potassium solubilizing bacterium, a nodule bacterium, actinomyces, a silicate bacterium, trichoderma sp., microzyme, a photosynthetic bacterium, calcium humate, zinc humate, magnesium humate, iron humate, copper humate, and manganese humate used as raw materials, carrying out tank continuous fermentation to allow the raw materials become thoroughly decomposed; carrying out deodorizing and removing water; and carrying out granulation to obtain the granular biological organic fertilizer containing medium trace element chelates of humic acid. The fertilizer has a complete range of nutrient elements and can fully satisfy needs of a crop for a fertilizer during growth.

The invention discloses an organic fertilizer fermentation method which adopts pool type aerobic continuous fermentation. The method specifically comprises the following steps: A, uniformly mixing solid organic wastes, a microbial fermentation bacterial agent and auxiliary materials in proportion to obtain a fermentation raw material, wherein the solid organic wastes, the microbial fermentation bacterial agent and the auxiliary materials are in a ratio of (70-75) to (29.99-24.99) to 0.01, so that the carbon nitrogen ratio in the fermentation raw material is (25-30) to 1; B, carrying out primary aerobic fermentation not less than 7 days on the fermentation raw material at the temperature not lower than 55 DEG C to obtain a primary fermentation product; C, carrying out secondary fermentation on the primary fermentation product at normal temperature to obtain a secondary fermentation product within 10-15 days; and D, drying the secondary fermentation product to obtain an organic fertilizer. By using fresh chicken manure as a main fermentation raw material, the organic fertilizer fermentation method disclosed by the invention achieves the purposes of harmless treatment, recycling treatment and reducing treatment.

The invention discloses a stair-stepping tea continuous-fermentation device. The stair-stepping tea continuous-fermentation device at least comprises a fermentation group. The fermentation group comprises a frame and at least two fermentation units. The at least two fermentation units are stepwise arranged from top to bottom. Each one of the at least two fermentation units comprises a fermentation tank, a discharge device, a scattering device and a conveyer device. The fermentation tank is provided with an air chamber communicated with an inner cavity of the fermentation tank. A bottom cover is arranged at the bottom of the fermentation tank. The air chamber is annular. The top and the bottom of the air chamber are sealed. A side of the air chamber is provided with a ventilation pipe communicated with an external ventilation device. An atomizing nozzle is arranged in the ventilation pipe. A temperature sensor and an oxygen concentration sensor are arranged in the inner cavity of the fermentation tank. Through the fermentation tank having two or more layers and through a cycle flow of primary fermentation, scattering and secondary fermentation, fermentation is more uniform and thorough. Through the air chamber having an annular structure, air convection ventilation is formed in the air chamber so that uniform fermentation is realized. Uniformity and consistency of quality of black tea fermented by the stair-stepping tea continuous-fermentation device are guaranteed.