58results about How to "High spore rate" patented technology

A method for preparing solid natto bacillus microecological agent by solid fermenting is disclosed, which belongs to the biological engineering technique field. The strain is separated from natto as natto bacillus which is named as Bacillus natto S703 and the preservation number of the strain is CCTCC M207147. The sieving method comprises the following steps: utilizing the character that the natto bacillus produces natto kinase to do dilute flat separation for natto leach, seeding the obtained single colony in the primary sieving culture medium of the flat which contains fiber, selecting single colony to purify and obtain the fitful natto bacillus according to the size of transparent coil diameter and colony diameter. The strain is inoculated in the solid ferment culture medium which comprises bran, soya bean waste, corn starch, dipotassium hydrogen phosphate and magnesium sulfate, which can produce the natto bacillus microecological agent by solid fermenting, drying and grinding. The prepared natto bacillus microecological agent can be chicken feed, which is provided with obvious modification for daily chicken weighing, feed coefficient and survival rate.

The invention discloses a high-density fermenting method of bacillus coagulans for livestock and poultry, as well as a preparation prepared by the method and application thereof. The strain collection number of the bacillus coagulans is CICC (China Center of Industrial Culture Collection) No.20138. The method comprises the following steps: performing liquid fermentation on the bacillus coagulans;performing solid alternating temperature continuous fermentation; ventilating in later phase of solid fermentation; continuing fermenting and drying, and preparing a bacillus coagulans preparation initial product after the drying is completed; further crushing and screening to obtain a high-density bacillus coagulans preparation final product, wherein the number of strains of the final product is115.5*10<8>cfu.g<-1>, and the spore rate is 77.2%. The product has the effects of increasing the weight and preventing diarrhea of piglet, and the application adding concentration is 0.05wt%. The number of strain preparation is far higher than that of similar strain preparations (20*10<8>cfu.g<-1>) in the market, so that the preparation has wide market competitively in the livestock and poultry breeding industry.

The invention discloses a bacillus subtilis fermenting method with a high rate of maturing gemma and belongs to the field of microorganism fermentation engineering. In the method, the target products are produced by activation of bacillus subtilis and two-step fermenting culture. The method comprises the following steps of: inoculating the activated bacillus subtilis first, performing fermentation culture for 8 to 14 hours under the conditions that: the culture temperature is between 35 and 38 DEG C, the cylinder pressure is between 0.02 to 0.06 Mpa, the ventilation rate is 1:0.5-1:1 and the stirring revolution is between 150 and 200 rpm to obtain seed bacterial solution, and inoculating the seed bacterial solution and culturing for 20 to 36 hours to obtain bacillus subtilis liquid products with a high rate of maturing gemma. In the method, the components of the culture medium can be easily obtained and have low price, process parameters are simple, the fermentation period is short, the obtained bacillus subtilis is high in number of living bacteria as well as rate of maturing gemma, the rate of maturing gemma reaches more than 3.0*10<9>CFU / ml, and the number of maturing gemma and the quality of the products are obviously improved.

The invention relates to a fermentation method of bacillus megatherium with the high rate of sporation. The method comprises the following steps that 1, the bacillus megatherium is inoculated into a solid medium after being activated, static culture is conducted, and secondary activated bacillus megatherium is obtained; 2, the secondary activated bacillus megatherium is inoculated into a seed medium, fermentation culture is conducted, and seed bacterial liquid is prepared; 3, the seed bacterial liquid is inoculated into a fermentation medium, fermentation culture is conducted, supplementary food liquid is added, fermentation is conducted continuously, and bacillus megatherium fermentation liquor is prepared. According to the fermentation method of the bacillus megatherium with the high rate of sporation, culture conditions and medium components are selected, and meanwhile sporation accelerants of bacillus megatherium are added in the fermentation process, the viable count and the rate of sporation of the bacillus megatherium are increased, and the viable count in the prepared fermentation liquor can reach above 8.0*109 CFU / ml; by means of oil lens observation after crystal violet staining, the rate of sporation is above 95 percent, the spore count of a product can be significantly increased, and the product quality of the product can be significantly improved.

The invention relates to the field of an agricultural microbial ecological agent, and particularly relates to an agricultural microbial ecological agent for soil conditioning and plant calcium supplement and a production method thereof. The agent is prepared according to the following steps: with oyster shell powder as a basic raw material and molasses meal as a nutrient medium, at first, fermenting an oyster shell by using zymophyte, and then adding other useful formula bacteria according to a reasonable proportion to prepare the agricultural microbial ecological agent. The agent not only can recycle waste materials to solve environmental pollution, but also can achieve large-scale high-value development and utilization of oyster shells, greatly improve the yield of plants and provide an economic and efficient agricultural microbial ecological agent for the development of modern organic agriculture.

The invention belongs to the field of aquaculture and discloses a compound bacteria preparation and a preparation method and application thereof. The preparation method includes: (1), preparing seeds;(2), inoculating bacillus coagulans; (3), inoculating clostridium butyricum; (4), inoculating lactobacillus and saccaromycetes mixed seed liquid to obtain the compound bacteria preparation. The compound bacteria preparation can be applied to aquaculture and can effectively degrade nitrite in an aquaculture pond, purify water and promote healthy culture and efficient production of aquaculture industry of China.

The invention relates to a microbial pesticide containing beauveria bassiana and a preparation method of the microbial pesticide. The microbial pesticide containing beauveria bassiana is prepared from graphene, sodium alginate, protein powder, gelatin, water, spores of beauveria bassiana and plant oil; the weight ratio of graphene to sodium alginate to protein powder to gelatin to spores of beauveria bassiana to plant oil is 1:(10-100):(10-20):(10-20):(60-1,000):(50-140); the water content of the microbial pesticide containing beauveria bassiana is 7%-30%. The microbial pesticide prepared with the method can be stored for a long time and has a good effect.

The invention discloses an antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof. The antagonistic bacterium is bacillus amyloliquefaciens with an accession number of CGMCC No. 6491. According to the invention, bacterial strains with strong bacteriostatic activity are obtained by carrying out screening of the antagonistic bacterium on soil samples collected from a farmland cultivated for a plurality of years in a suburban area of Fukang, Xinjiang; then further screening and domestication are carried out, and the taxonomic status of obtained stains are preliminarily determined through determination of morphological characteristics and the 16 S rRNA sequence of the strains and phylogenetic analysis of the strains; moreover, in-depth research is carried out on characteristics like a bacteriostasis rate and stability of the fermentation broth of the bacterium. The antagonistic bacterium offers a new option for selection of antagonistic bacteria capable of controlling soil-borne diseases of vegetable; antagonistic action of the antagonistic bacterium exerts a prominent technical effect on treating tomato wilt, fusarium wilt of cucumber, phytophthora capsici leon and pepper anthracnose; and the antagonistic bacterium has wide application values.

The invention discloses a fermentation culture method for isaria fumosorosea. The method comprises two stages, i.e. liquid fermentation and solid fermentation. Each liter of liquid culture medium contains 10 to 30 grams of a carbon source, 5 to 25 grams of a nitrogen source, 50 to 250 milligrams of a trace element and 0.5 to 1.5 grams of base salt, and the pH value is 4 to 8. A solid culture medium is corn flour, rice, peanut meal, bran and soybean meal. The produced isaria fumosaroseus is very high in spore output, purity and spore viability, the whole fermentation culture period is about 20 days, and the method has the advantages of short production period, low cost, high efficiency and the like, and is applicable to large-scale production of the isaria fumosorosea.

The invention discloses a preparation method of paecilomyces lilacinus spore powder. The method comprises the following steps of: (1) preparing a paecilomyces lilacinus liquid fermentation broth; (2) recovering conidia from the paecilomyces lilacinus liquid fermentation broth; and (3) drying the recovered paecilomyces lilacinus conidia to obtain the paecilomyces lilacinus spore powder. According to the invention, the technological parameters for preparing paecilomyces lilacinus spore powder are screened and optimized to obtain optimal technological parameters for preparation, thus the yield and product quality of paecilomyces lilacinus spores are effectively improved. The preparation method of paecilomyces lilacinus spore powder, disclosed by the invention, has the advantages of high recovery rate, high spore content, high live spore rate, low water content and the like, and conforms to the requirements of large-scale industrial production.

The invention provides a method for producing a biocontrol bacillus amyloliquefaciens agent by liquid-solid biphasic fermentation, wherein the method comprises the following steps: bacterial strain activation, shaking flask seed culture, deep liquid fermentation culture with a fermentation tank, solid fermentation culture with a porous shallow dish and fermentation post-treatment. The liquid fermentation culture medium comprises the components: bran, soybean powder, NaCl, MgSO4.7H2O and CaCO3, and the solid fermentation culture medium mainly contains bran, corncob powder, soybean meal, sucroseor molasses, and rice hulls. The number of effective living bacteria in the obtained bacterial agent product is more than or equal to 5.0*10<10> cfu / g, and the spore rate is 95% or more. The method has the advantages of simple process, low production cost, easy control and management, high yield of living bacteria and spores, and simple post-treatment, and is beneficial to application and promotion of the biocontrol microbial agent.

The invention relates to the field of microbial fermentation, and in particular relates to a bacillus coagulans solid-state fermentation production method. The method comprises the following steps: (1) first-stage seed preparation; (2) second-stage solid-state seed inoculation; (3) third-stage solid-state seed inoculation; and (4) solid-state fermentation inoculation, wherein a formula of a solid-state fermentation material comprises the following components in percentage by mass: 70-99% of wheat bran, 1-30% of soybean meal, 0.1-10% of glucose, 0.1-10% of urea, 0.1-10% of calcium carbonate and 0.1-5% of manganese sulfate, and accessory materials are added corresponding to the mass of the wheat bran and the soybean meal. According to the method disclosed by the invention, the solid-state fermentation inoculation quantity can be reduced by 50-95% compared with that of the prior art, and a pH value is kept between 7 and 8 in a fermentation process; moreover, a high bacterium number can be achieved, dominant populations can be formed for fermentation, bacterial contamination cannot be easily caused, relatively high spore numbers and spore rates can be achieved, the spore number is 1*10<9>CFU / g to 1*10<11>CFU / g, and the maximum spore rate can reach 99%; and the method is easy in production and promotion.

The invention provides a preparation method and application of a high activity biological pesticide microcapsule sustained release agent, and belongs to the field of crop disease prevention and control. The high-density culture of bacillus is achieved by the method of low-frequency electromagnetic field radiation treatment of a seed solution and stepwise feeding fermentation, and the viable countand the spore rate of the bacillus is significantly increased, and the microcapsule sustained release agent is prepared through the novel microcapsule technology with the bacillus as the active ingredient. Compared with the traditional biological pesticide formulation, the formulation can maintain the activity of the active ingredient for a long time, increase the storage stability of bacteria, prolong the shelf life of products, significantly improve the stability of the disease prevention, solve the problem of dust pollution and is environmentally friendly. If the biopesticide is used for controlling diseases such as soybean sclerotium diseases, and the prevention effects thereof are not significantly different from conventional chemical agents, and the biopesticide is environmentally friendly and suitable for industrial production promotion.

The invention relates to a bacillus subtilis fermentation medium which is prepared from the following components in percentage by mass: 0.5-2% of corn flour, 1-3% of peptone, 1-5% of biological nitrogen source, 0.5-2% of sucrose, 0.01-0.1% of magnesium sulfate, 0.1-1% of manganese sulfate, 0.1-1% of dipotassium phosphate, 0.01-0.1% of monopotassium phosphate and the balance of water. The medium can promote good growth of bacillus subtilis and increase the spore production rate of bacillus subtilis while the cost is low.

The invention discloses a Bacillus coagulans strain and application thereof. A preservation number of the Bacillus coagulans strain is CGMCC No.17374. The Bacillus coagulans strain is applied to microbial preparation field. The screened strain has the characteristics of highly producing spores and acid; the strain has obvious difference with other congeneric strains, formation rate of the spores is very high, the heat resistance is good, and the fermentation level is high; the strain is a probiotic and has the effects of maintaining balance of intestinal tracts, stimulating the immune and improving the health level of bodies same as normal lactic acid bacteria. A capsule preparation prepared by taking the strain as a core component has a good treatment effect and a function of regulating multiple intestinal discomforts.

The present invention discloses a method for high density production of bacillus amyloliquefaciens by combination of liquid and semisolid fermentation technologies, the method is as follows: liquid fermentation, to be more specific, a bacillus amyloliquefaciens seed liquid is inoculated into a liquid fermentation medium, the inoculation amount is 1-5%, and a liquid fermentation product is obtained by culturing for 20-26h under the conditions of temperature of 35-38 DEG C, the air inlet amount of 25-35L / min and the pressure of 0.02-0.04Mpa; and semi-solid fermentation, to be more specific, the liquid fermentation product is inoculated into a semisolid fermentation medium containing coco coir powder and cassava residue powder for even mixing, and a semi-solid fermentation product is obtained by culturing for 4-6 days under the conditions of temperature of 35-38 DEG C, the humidity of 50-70% and the inoculation amount of 18-22%; and by combination of the liquid and semisolid fermentation technologies and process parameter optimization, the bacteria content of obtained bacillus amyloliquefaciens final product is 3.21*10<12>cfu / g, and the rate of sporation is up to 93%.

The invention discloses a method for cultivating bacillus subtilis with a water-soluble raw material, relating to the technical field of microorganisms. Bacillus subtilis is used as a strain and is activated and then inoculated into a fermentation culture medium for cultivation, wherein the fermentation culture medium is prepared from 3%-4% of dextrin, 0.1%-0.2% of glucose, 3.2%-4% of corn starchpowder, 0.5%-0.8% of fish peptone, 0.9%-1.5% of additive, and 89.5%-92.3% of water. The method adopts a water-soluble, low-cost culture medium formula, so that the fermentation density of the bacillussubtilis is improved, the production cycle is shortened, the spore rate is high, the fermentation solution contains no insoluble impurities so as to be processed conveniently in following steps, andthe industrial production cost is lowered.

The invention discloses a preparation method of a bacillus licheniformis and butyric acid bacterium composite bacterial agent, and belongs to the technical field of biological fermentation. Accordingto the method, bacillus licheniformis secondary seed liquid is inoculated into a fermentation tank; when the viable count during the microscopic examination is 1.0*10<9> to 3.0*10<9>CFU / ml, the butyric acid bacterium secondary seed liquid is inoculated into a fermentation tank according to the proportion of the weight percentage being 2 to 6 percent; when the butyric acid bacterium is fermented to13 to 15h, a glucose solution starts to be added in a flowing manner; when the butyric acid bacterium is fermented to 14 to 16h, a nitrogen source supplementary material culture medium starts to be added in a flowing manner. The method provided by the invention uses a combined culture mode, the mutualism among microbes is utilized; the production cost is reduced; the production benefits are improved; the maintenance of the animal microecosystem is facilitated; the digestive absorption rate of the feed is improved through various kinds of secreted digestive enzymes and organic acids; the method can be applied to feed additives.

The invention discloses a biological pesticide containing soy sauce residue waste oil glyceride and a preparation method thereof. The biological pesticide comprises the following components in parts by weight: 20-30 parts of soy sauce residue waste oil glyceride, 40-50 parts of beauveria bassiana spore powder, 10-20 parts of xanthan gum, 5-10 parts of auxiliary stabilizer, 60-80 parts of diatomite, and appropriate amount of water. The biological pesticide has the advantages of being high in insecticidal activity, fast and stable in insecticidal effect, long in residual life, environment-friendly, efficient, low in toxicity and low in residue; the activity of the beauveria bassiana spore powder can be effectively maintained, the storage time of the spore is prolonged, and the live spore rate is improved, the biological pesticide stability is improved; and recycling of the soy sauce residue waste oil can be realized, the energy-saving and environment-protecting concept can be satisfied,and the more extensive application of the beauveria bassiana can be promoted.

The invention discloses a fermentation method for increasing the spore rate of bacillus licheniformis. The fermentation method comprises the two following stages: inoculating the bacillus licheniformis in a culture medium with the pH of 7.0-7.5, and carrying out fermentation culture for 20-26 hours at the temperature of 36-38 DEG C; then regulating and controlling the pH of fermentation liquid to 8.0-8.5, continuing carrying out fermentation culture at the temperature of 36-38 DEG C, stopping fermentation at the moment of 30-36 hours since inoculation, and harvesting thalli with the spore rate of more than 85%.

The invention discloses a screening method of bacillus coagulans capable of easily forming spores, and belongs to the technical field of microorganisms. The screening method comprises the following steps: repeatedly carrying out water bath-plate screening on rejuvenated bacillus coagulans for multiple times until the spore rate of the bacillus coagulans is not lower than 95%. According to the method, the rejuvenated bacillus coagulans are subjected to water bath method screening to produce spore individuals; then, the individuals, which do not form spores, are screened out through solid plate culture; multiple times of water bath-plate screening are repeated to improve the hereditary stability; the bacillus coagulans thalli, which easily form spores, are finally obtained through screening; and the spore rate after fermentation is improved while the yield is ensured. The method is simple, convenient to operate, capable of improving the spore forming capacity of the bacillus coagulans, not prone to strain degradation, capable of greatly improving the survival rate of the bacillus coagulans during high-temperature granulation and long-term storage, suitable for most common fermentation processes and beneficial to promoting production and application of the bacillus coagulans.

The invention discloses an industrial production method of insecticidal beauveria bassiana bacterium powder, which applies a culture medium of the insecticidal beauveria bassiana bacterium powder. Theculture medium comprises a slant culture medium, a second-grade liquid amplification culture medium and a third-grade solid amplification culture medium; the production method comprises four steps ofcarrying out slant strain culture, carrying out second-grade liquid amplification culture, carrying out third-grade solid amplification culture, drying and crushing. A whole protection technologicalprocess is complementary to each other and meets a self-fiber design mechanism (biological fermentation propagation), and the quality of the produced beauveria bassiana bacterium powder is stable; large-scale production is formed and wastes are reduced; the living spore rate of the produced beauveria bassiana bacterium powder is 85 percent or more, the living spore rate of the beauveria bassiana bacterium powder I is improved and the quality of a product is also improved by 20 percent; a production technology of an existing beauveria bassiana preparation is greatly improved; the yield and quality of beauveria bassiana are improved and the industrialization and standardization of the production of bacterium agents are realized.

The invention discloses a method for promoting clostridium butyricum to form spores and a microecological preparation and application thereof, and relates to the technical field of microbial preparations. The method for promoting the clostridium butyricum to form the spores comprises the steps that a spore production inducer is added in the fermentation culture process, the spore production inducer comprises inactivated clostridium butyricum bacterial sludge, and the inactivated clostridium butyricum bacterial sludge is obtained by inoculating clostridium butyricum into an induction culture medium to be cultured and inactivated and then conducting solid-liquid separation. According to the application, the situation that part of thalli which form spores firstly release substances such as spore production delay protein and the like to inhibit other thalli from forming spores due to an overlong spore forming process can be effectively prevented, so that the viable bacterium amount and the spore rate of the clostridium butyricum are effectively improved, the problem that high bacterium amount and high spore rate of the clostridium butyricum are difficult to coexist is solved, and the yield of the clostridium butyricum is improved. The preservation, the transportation and the use of the clostridium butyricum are greatly promoted; in addition, the microecological preparation prepared by the invention has a remarkable effect when being applied to piglet feeding, and can effectively improve the feed intake, promote digestion and reduce the occurrence of diarrhea.

The invention provides a bacillus subtilis preparation and a preparation method thereof. The preparation method comprises the following steps of: inoculating bacillus subtilis liquid into a fermentation medium, and cultivating to obtain fermentation liquid; and adding zeolite powder into the fermentation liquid, mixing uniformly and performing spray drying to obtain the bacillus subtilis preparation. According to a formula, the fermentation medium is prepared from the following materials in percentage by mass / volume: 1.0 to 2.0 percent of corn starch, 1.0 to 2.0 percent of low-temperature bean flour, 0.2 to 0.5 percent of antifoaming agent and the balance of water. The bacillus subtilis preparation has the advantages of high viable bacteria concentration and high sporation rate; and the preparation method for the bacillus subtilis preparation is easy to operate and low in cost.

The invention relates to the technical field of microorganisms, in particular to anti-helicobacter pylori bacillus coagulans BC99 and application thereof. The bacillus coagulans BC99 provided by the invention has relatively strong helicobacter pylori inhibition capability, and when the bacillus coagulans BC99 is co-cultured with helicobacter pylori, the viable count of the helicobacter pylori can be effectively reduced, the growth amount of the helicobacter pylori can be reduced, and the urease activity of the helicobacter pylori can also be remarkably reduced. Meanwhile, the bacillus coagulans BC99 has high self-agglutination capacity and copolymerization capacity with helicobacter pylori, adhesion of pathogenic bacteria in a body is hindered, and it is shown that the bacillus coagulans has high pathogenic bacteria removing capacity. The invention provides application of bacillus coagulans BC99 in preparation of anti-helicobacter pylori products and products for treating gastritis, gastric ulcer or gastric cancer.

The invention belongs to the field of microbes, and particularly relates to a method for continuously culturing bacillus subtilis and a special fermenting system. The method provided by the invention comprises the following steps: (1) transferring stored bacillus subtilis strain to a test tube agarslantculture-medium, performing strain activation, and then, transferring the strain into a triangular flask and a seeding tank for culturing in sequence to prepare a bacillus subtilis seed; (2) taking culture materials including starch, corn powder, soya bean meal, peptone, NaCl, CaCl2, MgSO4.7H2O, KH2PO4, K2HPO4, MnSO4, a defoaming agent and water; (3) after the culture materials in the step (2) are subjected to sterilizing treatment, inoculating the activated and cultured bacillus subtilis seed, and performing three-stage continuous culturing to obtain a fermenting liquid. The bacillus subtilis cultured with the method provided by the invention is stable and uniform in quality; neither mixed fungi pollution nor strain mutation is easily caused; the service life of various detectors is prolonged; the time is reduced; the yield and the production efficiency are improved; the concentration and the spore rate of thalli of the bacillus subtilis are improved.

The invention provides a Bacillus megaterium strain LXB4070 and application thereof. The preservation number of the Bacillus megaterium strain LXB4070 is CGMCC NO. 21867. The Bacillus megaterium strain LXB4070 has remarkable growth-promoting characteristics of phosphate solubilizing, potassium promoting, silicon solubilizing and the like, and basically does not have a biocontrol characteristic. A biocontrol growth-promoting microbial agent comprises the Bacillus megaterium strain LXB4070 and / or a fermentation product thereof, not only retains the original remarkable growth-promoting characteristics of phosphate solubilizing, potassium promoting, silicon solubilizing and the like, but also has efficient biocontrol characteristic, has efficient inhibiting ability on fusarium oxysporum, ginger stem basal rot pathogenic bacteria, botrytis cinerea, apple fusarium pathogenic bacteria and potato black scurf pathogenic bacteria, and has obvious growth-promoting effect on green Chinese onion, apples, grapes, strawberries, potatoes and the like and obvious gain effect on fruits.

The invention provides a preparation method of a spore preparation of bacillus coagulans. The preparation method of the spore preparation of bacillus coagulans comprises the following steps of: (a) slope thallus activation; (b) seeding tank cultivation of the bacillus coagulans; and (c) solid fermentation of the bacillus coagulans: placing the culture medium in the step (b) in a solid fermentation medium based on the weight ratio of 1%-10%, and fermenting for 48-72hours in a semi-closed manner. By using a semi-closed solid fermentation method, materials are in the semi-closed state in the process of fermentation and contact with a small amount of air, the bacillus coagulans can be propagated greatly by fully utilizing residual oxygen in the growing period, oxygen is greatly reduced in the later growing period, spore can grow rapidly, and more L-lactic acid can be metabolized, so that the problem that produced acid is insufficient in the fermentation process is solved, the growth of infectious microbe is inhibited through oxygen consumption, and the phenomenon of solid fermentation pollution is avoided.

The invention provides a fermentation method of a bacillus velezensis strain SUNO-18S-36, and relates to the field of microorganisms. According to the fermentation method, a culture medium is adopted and comprises 4-10 g / L of glucose, 5-20 g / L of corn steep liquor, 5-25 g / L of yeast powder, 5-10 g / L of silkworm chrysalis meal, 0-10 g / L of calcium carbonate, 0-30 g / L of calcium gluconate, 0.1-1 g / L of magnesium sulfate heptahydrate, 0-0.1 g / L of manganese sulfate and 0-10 g / L of calcium phosphate, and the pH is 7.0-7.5. The microorganisms involved in the fermentation method have the functions of dissolving phosphorus and degrading pesticide residues and have an antagonistic effect on pathogenic bacteria, and by adopting the fermentation method, fermentation liquor with high viable bacterium content, high spore rate and high bacteriostatic active substances can be obtained within a relatively short time.