58results about How to "High spore rate" patented technology

A method for preparing solid natto bacillus microecological agent by solid fermenting is disclosed, which belongs to the biological engineering technique field. The strain is separated from natto as natto bacillus which is named as Bacillus natto S703 and the preservation number of the strain is CCTCC M207147. The sieving method comprises the following steps: utilizing the character that the natto bacillus produces natto kinase to do dilute flat separation for natto leach, seeding the obtained single colony in the primary sieving culture medium of the flat which contains fiber, selecting single colony to purify and obtain the fitful natto bacillus according to the size of transparent coil diameter and colony diameter. The strain is inoculated in the solid ferment culture medium which comprises bran, soya bean waste, corn starch, dipotassium hydrogen phosphate and magnesium sulfate, which can produce the natto bacillus microecological agent by solid fermenting, drying and grinding. The prepared natto bacillus microecological agent can be chicken feed, which is provided with obvious modification for daily chicken weighing, feed coefficient and survival rate.

The invention discloses a bacillus subtilis fermenting method with a high rate of maturing gemma and belongs to the field of microorganism fermentation engineering. In the method, the target products are produced by activation of bacillus subtilis and two-step fermenting culture. The method comprises the following steps of: inoculating the activated bacillus subtilis first, performing fermentation culture for 8 to 14 hours under the conditions that: the culture temperature is between 35 and 38 DEG C, the cylinder pressure is between 0.02 to 0.06 Mpa, the ventilation rate is 1:0.5-1:1 and the stirring revolution is between 150 and 200 rpm to obtain seed bacterial solution, and inoculating the seed bacterial solution and culturing for 20 to 36 hours to obtain bacillus subtilis liquid products with a high rate of maturing gemma. In the method, the components of the culture medium can be easily obtained and have low price, process parameters are simple, the fermentation period is short, the obtained bacillus subtilis is high in number of living bacteria as well as rate of maturing gemma, the rate of maturing gemma reaches more than 3.0*10<9>CFU/ml, and the number of maturing gemma and the quality of the products are obviously improved.

The invention relates to the field of an agricultural microbial ecological agent, and particularly relates to an agricultural microbial ecological agent for soil conditioning and plant calcium supplement and a production method thereof. The agent is prepared according to the following steps: with oyster shell powder as a basic raw material and molasses meal as a nutrient medium, at first, fermenting an oyster shell by using zymophyte, and then adding other useful formula bacteria according to a reasonable proportion to prepare the agricultural microbial ecological agent. The agent not only can recycle waste materials to solve environmental pollution, but also can achieve large-scale high-value development and utilization of oyster shells, greatly improve the yield of plants and provide an economic and efficient agricultural microbial ecological agent for the development of modern organic agriculture.

The invention discloses an antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof. The antagonistic bacterium is bacillus amyloliquefaciens with an accession number of CGMCC No. 6491. According to the invention, bacterial strains with strong bacteriostatic activity are obtained by carrying out screening of the antagonistic bacterium on soil samples collected from a farmland cultivated for a plurality of years in a suburban area of Fukang, Xinjiang; then further screening and domestication are carried out, and the taxonomic status of obtained stains are preliminarily determined through determination of morphological characteristics and the 16 S rRNA sequence of the strains and phylogenetic analysis of the strains; moreover, in-depth research is carried out on characteristics like a bacteriostasis rate and stability of the fermentation broth of the bacterium. The antagonistic bacterium offers a new option for selection of antagonistic bacteria capable of controlling soil-borne diseases of vegetable; antagonistic action of the antagonistic bacterium exerts a prominent technical effect on treating tomato wilt, fusarium wilt of cucumber, phytophthora capsici leon and pepper anthracnose; and the antagonistic bacterium has wide application values.

The invention discloses a fermentation culture method for isaria fumosorosea. The method comprises two stages, i.e. liquid fermentation and solid fermentation. Each liter of liquid culture medium contains 10 to 30 grams of a carbon source, 5 to 25 grams of a nitrogen source, 50 to 250 milligrams of a trace element and 0.5 to 1.5 grams of base salt, and the pH value is 4 to 8. A solid culture medium is corn flour, rice, peanut meal, bran and soybean meal. The produced isaria fumosaroseus is very high in spore output, purity and spore viability, the whole fermentation culture period is about 20 days, and the method has the advantages of short production period, low cost, high efficiency and the like, and is applicable to large-scale production of the isaria fumosorosea.

The invention discloses a preparation method of paecilomyces lilacinus spore powder. The method comprises the following steps of: (1) preparing a paecilomyces lilacinus liquid fermentation broth; (2) recovering conidia from the paecilomyces lilacinus liquid fermentation broth; and (3) drying the recovered paecilomyces lilacinus conidia to obtain the paecilomyces lilacinus spore powder. According to the invention, the technological parameters for preparing paecilomyces lilacinus spore powder are screened and optimized to obtain optimal technological parameters for preparation, thus the yield and product quality of paecilomyces lilacinus spores are effectively improved. The preparation method of paecilomyces lilacinus spore powder, disclosed by the invention, has the advantages of high recovery rate, high spore content, high live spore rate, low water content and the like, and conforms to the requirements of large-scale industrial production.

The invention provides a preparation method and application of a high activity biological pesticide microcapsule sustained release agent, and belongs to the field of crop disease prevention and control. The high-density culture of bacillus is achieved by the method of low-frequency electromagnetic field radiation treatment of a seed solution and stepwise feeding fermentation, and the viable countand the spore rate of the bacillus is significantly increased, and the microcapsule sustained release agent is prepared through the novel microcapsule technology with the bacillus as the active ingredient. Compared with the traditional biological pesticide formulation, the formulation can maintain the activity of the active ingredient for a long time, increase the storage stability of bacteria, prolong the shelf life of products, significantly improve the stability of the disease prevention, solve the problem of dust pollution and is environmentally friendly. If the biopesticide is used for controlling diseases such as soybean sclerotium diseases, and the prevention effects thereof are not significantly different from conventional chemical agents, and the biopesticide is environmentally friendly and suitable for industrial production promotion.

The invention discloses a Bacillus coagulans strain and application thereof. A preservation number of the Bacillus coagulans strain is CGMCC No.17374. The Bacillus coagulans strain is applied to microbial preparation field. The screened strain has the characteristics of highly producing spores and acid; the strain has obvious difference with other congeneric strains, formation rate of the spores is very high, the heat resistance is good, and the fermentation level is high; the strain is a probiotic and has the effects of maintaining balance of intestinal tracts, stimulating the immune and improving the health level of bodies same as normal lactic acid bacteria. A capsule preparation prepared by taking the strain as a core component has a good treatment effect and a function of regulating multiple intestinal discomforts.

The invention discloses a preparation method of a bacillus licheniformis and butyric acid bacterium composite bacterial agent, and belongs to the technical field of biological fermentation. Accordingto the method, bacillus licheniformis secondary seed liquid is inoculated into a fermentation tank; when the viable count during the microscopic examination is 1.0*10<9> to 3.0*10<9>CFU/ml, the butyric acid bacterium secondary seed liquid is inoculated into a fermentation tank according to the proportion of the weight percentage being 2 to 6 percent; when the butyric acid bacterium is fermented to13 to 15h, a glucose solution starts to be added in a flowing manner; when the butyric acid bacterium is fermented to 14 to 16h, a nitrogen source supplementary material culture medium starts to be added in a flowing manner. The method provided by the invention uses a combined culture mode, the mutualism among microbes is utilized; the production cost is reduced; the production benefits are improved; the maintenance of the animal microecosystem is facilitated; the digestive absorption rate of the feed is improved through various kinds of secreted digestive enzymes and organic acids; the method can be applied to feed additives.

The invention discloses a biological pesticide containing soy sauce residue waste oil glyceride and a preparation method thereof. The biological pesticide comprises the following components in parts by weight: 20-30 parts of soy sauce residue waste oil glyceride, 40-50 parts of beauveria bassiana spore powder, 10-20 parts of xanthan gum, 5-10 parts of auxiliary stabilizer, 60-80 parts of diatomite, and appropriate amount of water. The biological pesticide has the advantages of being high in insecticidal activity, fast and stable in insecticidal effect, long in residual life, environment-friendly, efficient, low in toxicity and low in residue; the activity of the beauveria bassiana spore powder can be effectively maintained, the storage time of the spore is prolonged, and the live spore rate is improved, the biological pesticide stability is improved; and recycling of the soy sauce residue waste oil can be realized, the energy-saving and environment-protecting concept can be satisfied,and the more extensive application of the beauveria bassiana can be promoted.

The invention discloses a screening method of bacillus coagulans capable of easily forming spores, and belongs to the technical field of microorganisms. The screening method comprises the following steps: repeatedly carrying out water bath-plate screening on rejuvenated bacillus coagulans for multiple times until the spore rate of the bacillus coagulans is not lower than 95%. According to the method, the rejuvenated bacillus coagulans are subjected to water bath method screening to produce spore individuals; then, the individuals, which do not form spores, are screened out through solid plate culture; multiple times of water bath-plate screening are repeated to improve the hereditary stability; the bacillus coagulans thalli, which easily form spores, are finally obtained through screening; and the spore rate after fermentation is improved while the yield is ensured. The method is simple, convenient to operate, capable of improving the spore forming capacity of the bacillus coagulans, not prone to strain degradation, capable of greatly improving the survival rate of the bacillus coagulans during high-temperature granulation and long-term storage, suitable for most common fermentation processes and beneficial to promoting production and application of the bacillus coagulans.

The invention discloses an industrial production method of insecticidal beauveria bassiana bacterium powder, which applies a culture medium of the insecticidal beauveria bassiana bacterium powder. Theculture medium comprises a slant culture medium, a second-grade liquid amplification culture medium and a third-grade solid amplification culture medium; the production method comprises four steps ofcarrying out slant strain culture, carrying out second-grade liquid amplification culture, carrying out third-grade solid amplification culture, drying and crushing. A whole protection technologicalprocess is complementary to each other and meets a self-fiber design mechanism (biological fermentation propagation), and the quality of the produced beauveria bassiana bacterium powder is stable; large-scale production is formed and wastes are reduced; the living spore rate of the produced beauveria bassiana bacterium powder is 85 percent or more, the living spore rate of the beauveria bassiana bacterium powder I is improved and the quality of a product is also improved by 20 percent; a production technology of an existing beauveria bassiana preparation is greatly improved; the yield and quality of beauveria bassiana are improved and the industrialization and standardization of the production of bacterium agents are realized.

The invention provides a bacillus subtilis preparation and a preparation method thereof. The preparation method comprises the following steps of: inoculating bacillus subtilis liquid into a fermentation medium, and cultivating to obtain fermentation liquid; and adding zeolite powder into the fermentation liquid, mixing uniformly and performing spray drying to obtain the bacillus subtilis preparation. According to a formula, the fermentation medium is prepared from the following materials in percentage by mass/volume: 1.0 to 2.0 percent of corn starch, 1.0 to 2.0 percent of low-temperature bean flour, 0.2 to 0.5 percent of antifoaming agent and the balance of water. The bacillus subtilis preparation has the advantages of high viable bacteria concentration and high sporation rate; and the preparation method for the bacillus subtilis preparation is easy to operate and low in cost.

The invention relates to the technical field of microorganisms, in particular to anti-helicobacter pylori bacillus coagulans BC99 and application thereof. The bacillus coagulans BC99 provided by the invention has relatively strong helicobacter pylori inhibition capability, and when the bacillus coagulans BC99 is co-cultured with helicobacter pylori, the viable count of the helicobacter pylori can be effectively reduced, the growth amount of the helicobacter pylori can be reduced, and the urease activity of the helicobacter pylori can also be remarkably reduced. Meanwhile, the bacillus coagulans BC99 has high self-agglutination capacity and copolymerization capacity with helicobacter pylori, adhesion of pathogenic bacteria in a body is hindered, and it is shown that the bacillus coagulans has high pathogenic bacteria removing capacity. The invention provides application of bacillus coagulans BC99 in preparation of anti-helicobacter pylori products and products for treating gastritis, gastric ulcer or gastric cancer.

The invention provides a Bacillus megaterium strain LXB4070 and application thereof. The preservation number of the Bacillus megaterium strain LXB4070 is CGMCC NO. 21867. The Bacillus megaterium strain LXB4070 has remarkable growth-promoting characteristics of phosphate solubilizing, potassium promoting, silicon solubilizing and the like, and basically does not have a biocontrol characteristic. A biocontrol growth-promoting microbial agent comprises the Bacillus megaterium strain LXB4070 and/or a fermentation product thereof, not only retains the original remarkable growth-promoting characteristics of phosphate solubilizing, potassium promoting, silicon solubilizing and the like, but also has efficient biocontrol characteristic, has efficient inhibiting ability on fusarium oxysporum, ginger stem basal rot pathogenic bacteria, botrytis cinerea, apple fusarium pathogenic bacteria and potato black scurf pathogenic bacteria, and has obvious growth-promoting effect on green Chinese onion, apples, grapes, strawberries, potatoes and the like and obvious gain effect on fruits.

The invention provides a fermentation method of a bacillus velezensis strain SUNO-18S-36, and relates to the field of microorganisms. According to the fermentation method, a culture medium is adopted and comprises 4-10 g/L of glucose, 5-20 g/L of corn steep liquor, 5-25 g/L of yeast powder, 5-10 g/L of silkworm chrysalis meal, 0-10 g/L of calcium carbonate, 0-30 g/L of calcium gluconate, 0.1-1 g/L of magnesium sulfate heptahydrate, 0-0.1 g/L of manganese sulfate and 0-10 g/L of calcium phosphate, and the pH is 7.0-7.5. The microorganisms involved in the fermentation method have the functions of dissolving phosphorus and degrading pesticide residues and have an antagonistic effect on pathogenic bacteria, and by adopting the fermentation method, fermentation liquor with high viable bacterium content, high spore rate and high bacteriostatic active substances can be obtained within a relatively short time.