23 results about "Microecosystem" patented technology

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The utility model relates to the technical field of afforestation frame, concretely relates to a municipal afforestation afforestation frame, including base, the top of base is provided with the water receiving groove, the bottom fixedly connected with three groups first water guide pole of water receiving groove, the bottom fixedly connected with drought resistant plant water tank of first water guide pole, the bottom screw thread connection of drought resistant plant water tank has two groups second water guide pole and the outside wall of second water guide pole is equipped with sealing rubber ring, the bottom rotatably connected with nectar plant mounting plate of second water guide pole, the top of second water guide pole is provided with a plurality of groups water leakage hole, drought resistant plant water tank and the inside of nectar plant mounting plate all are installed with square plant flowerpot, the back side of nectar plant mounting plate is provided with a plurality of groups insertion slot and is installed with bamboo cylinder in the inside of insertion slot. The utility model cooperates with the water receiving groove through base, cooperates with the different ecological niche plant of square plant flowerpot planting in, has formed the complete microecosystem.

This invention discloses a method for the synergistic metabolic analysis of multiple components of traditional Chinese medicine (TCM) integrated with the gut microbiota. After initializing the microbiota ecosystem, a solution of multi-component TCM extracts is continuously injected into a microfluidic chip. Stable isotope-labeled precursors are pulsed into a specific simulation region to perform spatial isotope encoding. A standing wave acoustic field is applied at the sampling node of the microfluidic chip, and multiphase fluid spatial splitting is performed using the difference in the primary acoustic radiation force experienced by the particles to derive cell-free TCM metabolites. The cell-free TCM metabolites are separated by chromatography and gas phase drift separation. A four-dimensional metabolic feature array is constructed, and the absolute value of the mass shift is calculated for spatial inverse decoding. Closed-loop feedback is triggered based on the rate of change of target metabolite ion abundance, increasing the amplitude of the standing wave acoustic field pressure to confine intestinal bacteria and performing in-situ enrichment of synergistic metabolic intermediates. This invention realizes the spatial metabolic trajectory tracing and network reconstruction of multiple components of TCM in a complex gut microbiota ecosystem.

The invention relates to a blueberry culture medium and a preparation method of the blueberry culture medium. The blueberry culture medium is prepared from, by volume, 40%-50% of decomposed organic matter, 20%-30% of natural mineral structuring agent, 5%-10% of biochar, 10%-15% of peat soil, 4%-6% of functional additive, 1%-2% of microbial agent and 0%-10% of rural soil. The core raw material decomposed organic matter is derived from agricultural waste, the cost is low, waste can be turned into wealth, and expensive peat soil is saved. The natural mineral structuring agent retains water and fertilizer, has an excellent physical structure, ensures the looseness and air permeability of the blueberry culture medium, and is beneficial to the growth of blueberry root systems. The functional additive enables the blueberry culture medium to maintain stable acidity and can provide mineral nutrition. The microbial agent can activate phosphorus, potassium and other elements in soil, fix nitrogen, generate plant stimulant for promoting growth, inhibit soil-borne diseases and form a healthy rhizosphere microecosystem.

The invention discloses a microbial inoculum ZL6 with effects of promoting growth of haloxylon ammodendron and regulating and controlling rhizosphere microflora and application of the microbial inoculum ZL6, and belongs to the technical field of microorganisms. According to the bacillus cereus ZL6 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microflora structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. The ZL6 is mixed and applied to a haloxylon ammodendron field, so that the content activities of organic carbon and available phosphorus in soil are remarkably improved by 84.78% and 73.27% respectively. The plant height is increased by 68.11%, and the root length is increased by 206.55%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL6 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The invention relates to a compound microbial agent, a preparation method, application and a system. The compound microbial agent is prepared from the following raw materials in parts by weight: 100 parts of a fermented and decomposed compound organic carrier; 0.5-5 parts by weight of a functional microbial flora; 5 to 15 parts by weight of a functional conditioner; the composite organic carrier is composed of cotton stalk decomposed substances, agricultural processing residues and livestock and poultry manure; the functional microbial flora comprises bacillus, actinomycetes and saccharomycetes. According to the invention, the resource utilization of agricultural wastes is realized, the cost is reduced, more importantly, the carriers are rich in organic matters and various nutrients, and an excellent living environment and continuous nutrition supply are provided for functional flora. A micro-ecological system with complementary functions and synergistic interaction is constructed on the level of flora, the microbial agent is fundamentally different from a single-strain microbial agent, and a material basis is provided for solving the complex problems of saline-alkali coexistence and weak microbial activity of saline-alkali soil.

This invention belongs to the field of soil microecology technology, specifically relating to a soil-grown mesh bag assembly for isolating roots and mycelia, and its preparation method. The mesh bag assembly includes large-pore, medium-pore, and small-pore mesh bags. The large-pore mesh bags are made of large-pore nylon mesh, the medium-pore mesh bags are made of medium-pore nylon mesh, and the small-pore mesh bags are made of hydrophilic composite filter membrane cloth. The sidewalls of the medium-pore and small-pore mesh bags are equipped with a composite sealing structure. This invention also provides a gradient isolation method for in-situ soil cultivation using this mesh bag assembly, and the corresponding preparation method. This invention, through multi-pore gradient design and a composite sealing structure, achieves selective physical isolation between roots and mycelia within the soil, highly restoring the in-situ microecological environment. It effectively solves technical problems such as root leakage during sewing and adhesive detachment, improving the effectiveness of experimental samples. It is suitable for in-situ cultivation research of various soil microecological systems and has broad scientific research application value.

The invention relates to the field of biomedical materials and animal feed additives, and particularly discloses a preparation method and application of micro-sized calcium alginate gel particles loaded with bidirectional functionalized nano-selenium. The preparation method comprises the following steps: (1) mixing nano-selenium with high molecular weight alginate to obtain alginic acid modified nano-selenium; (2) mixing the alginic acid modified nano-selenium with low-molecular-weight alginate, and performing spray drying to prepare nano-selenium alginic acid microspheres; and (3) mixing the nano-selenium alginic acid microspheres with a calcium ion solution, washing, and drying to form the micro-sized calcium alginate gel particles. The nano-selenium calcium alginate gel particles are micron-sized, can realize positioning slow release of gastrointestinal tracts, have the effects of resisting oxidation, enhancing immunity, regulating inflammation, improving intestinal microecology, resisting pathogenic microbial infection and the like, and can be used for selenium nutrition enrichment and resistance replacement / reduction breeding of animals.

The invention discloses a mixed bacterial agent ZL3 + 6 with effects of promoting growth of haloxylon ammodendron and regulating rhizosphere microflora and application of the mixed bacterial agent ZL3 + 6, and belongs to the technical field of microorganisms. According to the bacillus orientalis ZL3 and the bacillus cereus ZL6 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 and the ZL6 are mixed and applied to a haloxylon ammodendron field, the contents of hydrolytic nitrogen, available phosphorus and rapidly available potassium in soil and the activity of sucrase are remarkably improved and are respectively improved by 49.27%, 80.48%, 12.65% and 64.40%. The plant height is increased by 51.75%, and the root length is increased by 262.18%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, the ZL3 + 6 inoculant is inoculated to promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The invention discloses a micro-ecological system for purifying river water quality. The micro-ecological system comprises timber piles, functional ecological bags, geogrids and submerged plants, the micro-ecological system is round or square, the periphery of the micro-ecological system is fixed by timber piles, and a solar microporous aeration disc is arranged in the center of the micro-ecological system; at least one functional ecological bag is arranged in the micro-ecological system, modified functional filler is filled in the functional ecological bag, a geogrid is arranged above the functional ecological bag, the geogrid is fixedly connected with the timber piles on the periphery, and submerged plants are arranged on the geogrid. The system organically integrates the stability of civil engineering, the activity enhancement of environmental engineering and the natural restoration capability of ecological engineering, and realizes long-term, stable and low-energy-consumption purification of nitrogen, phosphorus, organic matters and other pollutants in river water.

This invention discloses a method for preparing and applying a compound fertilizer of filamentous microalgae and biochar for enhancing phosphorus utilization in saline-alkali land, belonging to the field of agricultural resource utilization. This invention achieves stable colonization and functional enhancement of microalgae in the porous structure of biochar through a three-step synergistic process of acid modification, ultrasonic assistance, and co-cultivation. Organic acids are creatively used to modify biochar, optimizing its pore structure and surface functional groups to improve microalgae adhesion. Then, ultrasonic assistance is used to clean the pores of the biochar, promoting the entry of microalgae cells into the pores. Finally, the modified biochar and filamentous microalgae are co-cultivated under light conditions, allowing the microalgae to actively grow and colonize the porous structure of the biochar, forming an integrated active microalgae-biochar compound fertilizer. It has advantages such as increasing the content of available nitrogen and potassium in the soil and significantly improving the bioavailability of existing phosphorus in the soil. Moreover, it can regulate the soil to a neutral range, constructing a healthy and long-lasting soil micro-ecosystem.

The present application belongs to the technical field of animal model construction, relates to the technical field of feed additive, and particularly relates to a prebiotic for constructing a human microbiota-associated (HMA) animal model and a preparation method thereof. The present application discloses a prebiotic for improving the colonization effect of human microbiota in the intestines of animals, which is composed of the following components in parts by weight: xylo-oligosaccharides 15-30 parts, 2'-fucosyllactose 5-15 parts, resistant starch 20-35 parts, inulin 10-20 parts, and carrier 8-15 parts. Through the synergistic effect of the specific prebiotic combination, the colonization fidelity and persistence of human microbiota in the animal model are significantly improved. And through multi-dimensional functional evidence (the similarity of the structure and metabolic spectrum of the microbiota), the successful reconstruction of the intestinal microecosystem is proved.

The present application belongs to the technical field of sewage treatment, and particularly relates to a preparation process of an iron salt-chitosan composite modified microbial high-adaptation type biochar. The present application combines an iron salt-chitosan composite modified biochar carrier with arsenic and antimony resistance functional bacteria, and the composite modified biochar not only retains excellent pore structure and specific surface area, but also forms a micro-ecosystem of nutrient release and micro-environment buffering through the synergistic effect between materials. The immobilized functional bacteria further degrade or transform As and Sb forms through biological transformation, realize the synergistic effect of multiple mechanisms, and greatly improve the removal efficiency of arsenic and antimony composite pollution. Moreover, the raw material biochar of the present application is widely available and low in cost, chitosan and iron salt are both environmentally compatible materials, the overall preparation process is green and environmentally friendly, and the prepared biochar can be applied to the treatment of high-concentration and complex-component arsenic and antimony contaminated wastewater, and has good application value.

The invention discloses a composite control method for micro-ecological intervention of stone mountain soil by coupling an electric field and a magnetic field, and relates to the technical field of ecological restoration. Aiming at structural heterogeneous characteristics such as interlayer fracture, low porosity and large specific resistance of the stone mountain soil, the method combines micro-area structure identification and conductive characteristic self-adaptive detection, and the micro-ecological intervention of the stone mountain soil is realized. An electromagnetic intervention adaptability factor model is constructed, and accurate recognition and classified layout of intervention blind areas are achieved. Through blanket-type, net-type and hybrid electromagnetic device layout strategies, in cooperation with dynamic adjustment of excitation frequency, power and layout spacing, enhanced intervention of a high-resistance risk area is realized. Micro-ecological activation indexes including microbial activity, rhizosphere growth rate and available nutrient release rate are collected, intervention response rate is calculated, intervention effect is evaluated in real time, and a multi-stage adjustment strategy is triggered. According to the method, the spatial uniformity and accuracy of intervention are promoted and improved, the defects of intervention blind areas and single excitation parameters are overcome, and the activity and recovery capability of the micro-ecosystem are enhanced.

The invention relates to the technical field of environmental treatment, in particular to a composite microbial preparation for water pollution treatment and a preparation method thereof. The composite microbial preparation for water pollution control is prepared from the following components in percentage by mass: 22 to 35 percent of magnetic targeting bacteria-algae symbiont, 5 to 6 percent of sodium carboxymethyl cellulose, 3 to 4 percent of charcoal nano powder, 35 to 40 percent of sodium alginate, 10 to 12 percent of polyvinyl alcohol, 2 to 3 percent of chitosan, 5 to 6 percent of trehalose, 3 to 4 percent of glycerol and 2 to 3 percent of calcium carbonate. The algal-bacterial symbiotic system disclosed by the invention has relatively high bacterial colony density, can efficiently degrade various pollutants in a polluted water body, can realize accurate positioning and enrichment treatment, realizes long-acting self-maintenance purification of water pollution treatment by combining with an algal-bacterial mutual benefit micro-ecological system, and is environment-friendly in all components, low in cost and easy to popularize. The risk of secondary pollution is avoided, and the unification of efficient treatment and environmental safety is realized.

This invention discloses a method for preparing and applying a compound fertilizer of filamentous microalgae and biochar for enhancing phosphorus utilization in saline-alkali land, belonging to the field of agricultural resource utilization. This invention achieves stable colonization and functional enhancement of microalgae in the porous structure of biochar through a three-step synergistic process of acid modification, ultrasonic assistance, and co-cultivation. Organic acids are creatively used to modify biochar, optimizing its pore structure and surface functional groups to improve microalgae adhesion. Then, ultrasonic assistance is used to clean the pores of the biochar, promoting the entry of microalgae cells into the pores. Finally, the modified biochar and filamentous microalgae are co-cultivated under light conditions, allowing the microalgae to actively grow and colonize the porous structure of the biochar, forming an integrated active microalgae-biochar compound fertilizer. It has advantages such as increasing the content of available nitrogen and potassium in the soil and significantly improving the bioavailability of existing phosphorus in the soil. Moreover, it can regulate the soil to a neutral range, constructing a healthy and long-lasting soil micro-ecosystem.

The invention relates to the technical field of deep processing of natural products, in particular to a fermentation preparation method of an active dandelion extract with functions of removing stasis and resisting inflammation, which comprises the following steps: crushing dried whole dandelion, and carrying out synergistic enzymolysis by using a compound enzyme consisting of cellulase, pectinase and beta-glucanase; then carrying out primary anaerobic fermentation of lactobacillus plantarum and secondary aerobic fermentation of issatchenkia orientalis in sequence; and finally, centrifuging, filtering, concentrating and spray-drying to obtain the high-activity dandelion fermentation extract. Through a process chain of ternary composite enzymolysis and step-by-step fermentation, an anaerobic-aerobic alternating micro-ecological system is constructed, and synergistic enrichment and functional quantification of double active components are realized. Specifically, in the final fermentation extract, the content of chicoric acid is greater than or equal to 8.5%, and the content of small molecular glycopeptide is greater than or equal to 25%, which are far higher than those of the traditional aqueous extract.

The invention provides a controllable specific-pathogen-free lateolabrax japonicus seedling raising method, and belongs to the technical field of aquaculture. The method comprises the following steps: performing gradient salinization domestication on lateolabrax japonicus parents with negative viruses in a specific pathogen management and control list, performing intensified ripening cultivation, performing virus negative screening to obtain sexually mature lateolabrax japonicus parents, performing induced spawning under sterile conditions, and collecting mature ova and semen for insemination; the obtained fertilized eggs are disinfected and subjected to virus negative screening, and then are hatched in a water body containing bacillus subtilis and nitrifying bacteria; and culturing the obtained larva fish in a micro-ecological system containing chlorella, bacillus subtilis and nitrifying bacteria, carrying out virus negative screening, then carrying out gradient bait transfer culture and continuous virus negative screening, and then, carrying out seedling emergence. According to the method, the comprehensive survival rate in the whole process reaches 28.6%, more than 12100,000 seedlings emerge in a single batch, specific pathogens in the whole process are zero-positive, the SPF seedling standard is met, and the breeding survival rate after deep sea net cage putting is increased by 32% compared with common seedlings.

The present application relates to the field of environmental water treatment technology, and discloses a water source denitrification ecological reaction cabin double-membrane micro-ecosystem, a construction method and application thereof. The method comprises the following steps: adding inorganic electron donor filler into a reactor; achieving an aerobic / anaerobic environment alternation through intermittent aeration; using intermittent water feeding to pass the reservoir source water into the reactor filled with inorganic electron donor filler; repeating the intermittent water feeding step for 3-5 times until an aerobic denitrification enhanced denitrification part is formed on the surface of the inorganic electron donor. The denitrification ecological reaction cabin double-membrane micro-ecosystem constructed by the present application effectively reduces the concentration of nitrogen pollutants and COD pollutants in the micro-polluted water body, solves the problem of efficiency reduction caused by the lack of electron donor in the water body, and meets the practical requirements of micro-polluted water body treatment.

The invention discloses a corrugated paper-based prawn circulating water treatment system and a use method, and relates to the technical field of prawn culture, the corrugated paper-based prawn circulating water treatment system comprises a culture pond, a physical filtering unit, a nitrification biological filter, a solid-phase denitrification reactor, a sterilization and temperature regulation unit and an oxygenation and pH regulation unit which are communicated in sequence. The core lies in that waste corrugated paper subjected to alkali-enzyme composite pretreatment is adopted as a solid carbon source to be filled in a denitrification reactor, and multiple optimization means such as radial flow gradient distribution design, temperature partition regulation and control, fiber gradation and progressive microbial acclimation are further adopted; and a micro-ecological system of which the carbon source release rate and the microbial denitrification demand are highly synergetic in time and space is constructed. According to the method, waste is recycled, efficient, stable and low-cost denitrification nitrogen removal is achieved, the risks of nitrate accumulation and harmful by-product generation are remarkably reduced, the effluent quality is excellent, operation and maintenance are easy and convenient, and the method is suitable for long-term stable operation of a prawn recirculating aquaculture system.

The invention discloses a stress-resistant growth-promoting carbon-based bio-organic fertilizer as well as a preparation method and application thereof, and belongs to the technical field of bio-organic fertilizers. The bio-organic fertilizer comprises the following raw materials in parts by weight: 40-60 parts of decomposed sheep manure, 20-30 parts of bean cakes, 15-25 parts of peanut shell charcoal, 3-5 parts of a functional microbial agent, 2-4 parts of a poplar bark extract, 5-10 parts of a mineral synergist and 2-5 parts of a binder. By compounding microorganisms and innovatively adding the poplar bark extract, all the components have a synergistic effect, a healthy rhizosphere microecosystem is provided for plants, growth is promoted by activating nutrients and inhibiting diseases, and the yield of the plants is increased. And the resistance of plants to abiotic stresses such as drought can be obviously enhanced by improving the rhizosphere soil environment and activating the intrinsic resistance of the plants. For the rare medicinal material ginseng with long growth cycle and high requirements on soil environment, the method is a very promising green and efficient agricultural technical measure.

The invention relates to the technical field of traditional Chinese medicinal material cultivation, and discloses a planting method of traditional Chinese medicinal materials. The method comprises the following steps: a land preparation stage: performing three-dimensional treatment on a selected land, including surface biomass cleaning, subsurface soil replacement and deep soil gas explosion; in the seed energizing stage, multiple rounds of sequential physical and biological treatment are carried out on the seeds; in the field planting and micro-domain construction stage, a special field planting device is adopted to complete seedling transplanting, and a root zone micro-ecological system is constructed synchronously; in a dynamic intervention stage in the growth cycle, according to a preset plant physiological signal model, asymmetrically regulating and controlling a canopy light environment and rhizosphere water, fertilizer, gas and heat composite conditions; and a postpartum system resetting stage, wherein systematic recovery operation including soil pulse disinfection and ecological niche resetting is carried out after harvesting. A whole-course closed-loop management system from land to products is formed, the growth environment of traditional Chinese medicinal materials can be systematically improved, plant health and quality formation are promoted, and ecological sustainability of a planting system is achieved.

The invention discloses a green-foot partridge chicken feeding method which is characterized in that a compound microecological preparation is added into daily ration of green-foot partridge chickens, and the compound microecological preparation is composed of bacillus subtilis, saccharomycetes and enterococcus faecalis. The feeding method disclosed by the invention has the effects of regulating the balance of the micro-ecosystem of animals, directly supplementing exogenous beneficial bacteria, inhibiting or killing harmful bacteria, enhancing the organism immunity of the animals, protecting the ecological environment and the like; the microecological preparation improves the feed utilization rate, improves the production performance, improves the disease resistance and generates beneficial metabolites and antibacterial substances through a dominant population action mechanism, a biological barrier action mechanism and a biological oxygen abstracting action mechanism; the quality of chicken products can be improved, the protein content is increased, the fat and cholesterol content is reduced, the fiber tissue of meat is changed, and the meat is fresh, tender and tasty without fishy smell; the feed additive has the effects of promoting growth and resisting diseases of antibiotics, and can overcome the defects of drug residues and drug resistance of the antibiotics.