124 results about "Methanobacter" patented technology

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiR lyase and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogen viruses from a large amount of rumen microbiome sequencing data, and high-dimensional homology between the proteins and PeiR proteins based on a deep learning model TM-Vec is further analyzed, so that a series of methanogen lyases are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

To provide a methanation system which has good efficiency of conversion of carbon dioxide to methane even when gas containing a low concentration of carbon dioxide is used as a carbon dioxide source, and which enables simplification of equipment and reduction of installation area thereof.SOLUTION: Methanation equipment is provided, comprising: a dissolution tank exposing carbon dioxide-containing gas to an aqueous phase, thereby producing carbon dioxide-containing solution; a culture tank for culturing methane in culture solution, thereby producing methane; a carbon dioxide gas supply flow passage for introducing carbon dioxide-containing gas into the dissolution tank; solution transfer means transferring the carbon dioxide-containing solution produced in the dissolution tank from the dissolution tank to the culture tank; a hydrogen supply flow passage for supplying hydrogen to the dissolution tank or the culture tank; and a methane discharge flow passage for discharging the produced methane from the culture tank.SELECTED DRAWING: Figure 1

The invention provides an intelligent optimization method for carbon emission reduction of an ecological pasture in extreme weather, and relates to the technical field of intelligent optimization of carbon emission reduction. Comprising the steps that initial monitoring data are collected and preprocessed, and multi-dimensional feature vector output state features are constructed; an LSTM time sequence prediction model is used for analyzing the state of the dairy cow and the activity of methanogens in the rumen, DHI data of an integrated gradient boosting tree model is used for analyzing the amino acid balance and precise nutrition technology of the lactating dairy cow, a dairy cow manure yield prediction model based on a Bayesian network is constructed, a manure management strategy is dynamically optimized, and manure management effect data is output. And constructing a carbon emission reduction comprehensive evaluation model, and evaluating the pasture carbon emission reduction effect. According to the invention, the rumen flora structure is regulated and controlled, the rumen greenhouse gas emission is controlled, the ruminant feed protein level is reduced, and the methane emission is reduced by researching the dairy cow carbon emission rumen microorganism regulation and control technology.

The invention relates to the technical field of wastewater resourceful treatment, in particular to a magnetic field reinforced anaerobic reactor and a wastewater resourceful treatment method. The magnetic field intensified anaerobic reactor comprises an anaerobic reactor body and a plurality of magnetic blocks fixed on the outer surface of the anaerobic reactor body, the plurality of magnetic blocks are divided into a plurality of rows and surround the outer surface of the anaerobic reactor body, and the magnetic blocks in each row are circularly arranged in the sequence of left, upper, right and lower according to the magnetic field direction. The magnetic field enhanced anaerobic reactor can utilize the magnetic field to convert the ascending or descending motion of microorganisms in the reactor depending on water flow, bubbles or self into magnetic induction line cutting motion, convert kinetic energy into electric potential energy and enhance electron transfer between acetogens and methanogens; the utilization degree of organic matters in the wastewater by the acid-producing bacteria and the methane-producing process of reducing carbon dioxide by utilizing electrons by the methane-producing bacteria are improved, and the energy utilization and methane-producing efficiency in the anaerobic reactor are improved.

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.

The utility model belongs to sewage treatment technical field, specifically is a kind of sludge reduction's sewage treatment equipment, including base, the base top is fixed with processing cylinder, the processing cylinder bottom is fixed with bottom plate, by setting movable cylinder, second movable shaft, stirring plate, stirring rod and stirring ring, can make sludge particle in sewage even suspended, let hydrolytic bacteria, methanogen etc. and the organic matter in sludge fully contact, accelerate hydrolysis, acidification and the rate of methanogenesis reaction, reduce substrate residue, and anaerobic sludge is kept for a long time, can form dense precipitate layer in processing cylinder bottom, lead to effective reaction volume shrinkage, stirring can make sludge keep suspended state, maintain the effective volume utilization rate of processing cylinder, while anaerobic treatment needs stable temperature, if not stirring, local temperature difference in processing cylinder is easy to appear, lead to microbial activity imbalance, by stirring can maintain the uniformity of sewage anaerobic environment, stable reaction condition.

The invention provides an agricultural waste hydrolytic acidification method and a method for synthesizing medium-chain fatty acid, and belongs to the technical field of agricultural waste resource utilization. The invention provides an agricultural waste hydrolytic acidification method which comprises the following steps: after adjusting the solid content of agricultural waste, mixing the agricultural waste with short-chain AHLs and DPD-loaded chitosan microspheres to obtain a hydrolytic acidification system; and carrying out anaerobic fermentation on the hydrolytic acidification system to obtain a hydrolytic acidification product. According to the method, short-chain AHLs are added in the hydrolytic acidification process of the agricultural wastes to activate related genes of hydrolytic acidification bacteria, so that biofilm formation and extracellular enzyme secretion are promoted, the hydrolytic acidification efficiency of a substrate is improved, and substrate decomposition and short-chain fatty acid accumulation are promoted; dPD is released in a delayed manner in the metabolic peak period of acid-producing bacteria, the metabolic division of hydrolytic bacteria and acid-producing bacteria is coordinated, the activity of methanogens is inhibited, substrate competition is reduced, the degradation rate of agricultural waste cellulose is increased, and the yield of medium-chain fatty acid precursor substances such as acetic acid in fermentation substrates is increased.

The present application relates to the technical field of excrement treatment, and particularly relates to a feces treatment method based on flora analysis, comprising obtaining reaction temperature and material Ph value and determining gas production relative deviation based on gas production, determining device operation state based on characteristic parameters; in response to the device operation state, determining abnormal characteristic identification to obtain key functional flora parameters, determining flora balance index based on the key functional flora parameters to determine whether acid-producing bacteria and methanogenic bacteria are in flora balance; determining smoothness index according to the change curve of a plurality of actual flora balance indexes obtained in an initial detection period to determine the deviation degree of the flora balance index, and adjusting the correction sensitivity coefficient of the feed adjustment amount based on the smoothness index. The present application performs real-time monitoring and dynamic regulation on the excrement treatment process based on the flora analysis result to maintain optimal flora structure and metabolic activity, and improves treatment efficiency and stability.

The invention provides a material and a method for cleaning biogas residues produced by anaerobic digestion of waste oil reservoirs. The method comprises the following steps: (1) determining required anaerobic digestion methanogens and substrates according to the types of biogas residues produced by anaerobic digestion of the waste oil reservoir; (2) the oil reservoir is screened according to the requirements of the anaerobic digestion methanogens and the substrate for temperature, mineral substances, pH value and permeability, and a standard rock core is obtained; (3) adopting a physical simulation method, injecting anaerobic digestion methanogens and different organic matters into the standard rock core to clean in a manner of producing biogas residues through anaerobic digestion, and performing biogas residue cleaning test on the biogas residues generated by the different organic matters to obtain a cleaning scheme; and (4) cleaning the biogas residues produced by anaerobic digestion of the waste oil reservoir according to the cleaning scheme. A classification cleaning strategy is designed according to the blocking risk of organic matter biogas residues of different sources, the method for preparing the biogas through anaerobic digestion of the waste oil reservoir can be achieved, and the in-situ biogas preparation efficiency is improved.

The invention discloses a binary pseudocapacitance composite material for promoting anaerobic digestion of kitchen waste and a preparation method and application thereof, and belongs to the technical field of organic solid waste recycling and renewable energy sources. The wood chip biochar-polydopamine-Fe3O4 composite material with a core-shell structure is constructed by taking wood chips as raw materials, preparing biochar through low-temperature pyrolysis, forming an interface modification layer through polydopamine coating, loading Fe < 3 + > and carrying out secondary pyrolysis to generate Fe3O4 nanoparticles (40-110nm). The material can be recovered through a magnetic field (the recovery rate is greater than or equal to 90%), when the dosage is 0.8-1.2 g / L, the peak value of volatile fatty acid in an anaerobic digestion system is obviously reduced to be less than or equal to 400 mg / L, the methane accumulation yield is improved to be greater than or equal to 45%, the electron transfer efficiency of acid-producing bacteria and methanogens is synchronously optimized, and the system operation is stabilized.

The application provides a method for enhancing anaerobic digestion of organic solid waste containing new pollutant antibiotics by circulating oxygen disturbance, and belongs to the technical field of solid waste resource treatment. The method introduces circulating and increasing micro-nano bubble oxygen water stress during the stable operation period of anaerobic digestion, uses the dynamic change of physical oxidation pressure to induce the adversity defense mechanism of microorganisms, and promotes the improvement of metabolic activity and optimization of community structure of functional flora in situ. By accurately regulating the key process parameters such as oxygen disturbance cycle, gradient dosing ratio and the like, the application not only avoids the excessive inhibition of oxygen on methanogens, but also significantly enhances the adaptability of the system to the environmental stress of new pollutants. The application has the advantages of no need for external agents, high physical mass transfer efficiency, significant yield increase and harmless effect, and is suitable for in-situ strengthening and deep performance improvement of anaerobic digestion systems of municipal sludge, livestock and poultry manure and kitchen garbage and the like.

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.

The invention discloses a method for rapidly constructing denitrification type anaerobic methane oxidation through hydrogen type methanogens, and relates to the technical field of sewage treatment. According to the method, anaerobic digestion sludge of a sewage treatment plant is taken for inoculation, and a methanogen-mediated denitrification type anaerobic methane oxidation process is constructed. Hydrogen-type methanogens in the anaerobic digestion system excite a reverse methanogenesis function under the condition that organic matters are deficient, and denitrification anaerobic methane oxidation is realized by coupling sulfate and nitrate with nitrogen and sulfur circulating microorganisms. Sulfate is used as a potential electron acceptor in sewage, and dissoluble methane is removed while sulfate is indirectly utilized through recessive biological sulfur cycle catalytic bridging anaerobic methane oxidation and denitrification. In addition, the anaerobic digestion sludge can be continuously inoculated with hydrogen-type methanogens, and compared with traditional denitrification type anaerobic methane oxidation archaea culture, the method is more convenient. Therefore, the denitrifying anaerobic methane oxidation rapidly constructed by the hydrogen-type methanogens is an effective measure for breaking through the deep denitrification of the sewage treatment plant and synchronously controlling and discharging the greenhouse gas.

The present invention discloses a method for enhancing methane production and carbon sequestration in anaerobic digestion by zero-valent iron in cooperation with exogenous CO2. The method includes: inoculating sludge in a reactor, adding zero-valent iron thereto, and then adding wastewater. The reaction temperature is maintained at 35 ± 1 °C, and CO2 is injected into the liquid surface at a flow rate of 1.5 L / min for 5 - 50 min / L under stirring. CO2 and zero-valent iron jointly regulate the pH of the system, providing a suitable anaerobic digestion environment for microorganisms. Through the corrosion reaction of zero-valent iron under anaerobic conditions, electrons are released and H2 is generated, stimulating the proliferation of hydrogen-consuming microorganisms, while improving the activity of microorganisms and enzymes, increasing the methane production and rate. In addition, some methanogens in the system utilize H2 and CO2 to produce amorphous carbon, and zero-valent iron and exogenous CO2 are used to regulate the growth of amorphous carbon, strengthening the fixation of CO2 in the system.

The present invention provides a method for producing methane by dry anaerobic fermentation, which relates to the technical field of anaerobic fermentation. In the present invention, a biochar-immobilized complex enzyme is added during the dry anaerobic fermentation process. The biochar-immobilized complex enzyme is achieved by fixing the enzyme to biochar, which can enhance the stability and resistance to protein degradation of the enzyme, extend the service life of the enzyme, and reduce the inactivation rate of the enzyme, thereby facilitating an increase in methane production. In addition, biochar has a high specific surface area and the characteristics of promoting interspecies electron transfer, which is conducive to increasing methane production. Moreover, in the biochar-immobilized complex enzyme, biochar fixes the enzyme on its surface or interior, which can provide more reactive sites, which is conducive to the enrichment of methanogens, thereby shortening the fermentation reaction cycle and facilitating an increase in the reaction rate of anaerobic fermentation. In summary, the reaction rate and methane yield of the method for producing methane by dry anaerobic fermentation provided by the present invention are both high.

The invention provides a method for preparing biogas by anaerobic digestion of a waste oil reservoir, which comprises the following steps of: determining required anaerobic digestion methanogens and substrates according to geological characteristics, temperature, salinity and residual oil components of a target waste oil reservoir; the abandoned oil reservoirs are screened through the geological parameters and the well pattern mode, and target oil reservoirs are obtained; arranging a well pattern form in which vertical wells and horizontal wells are combined on a target oil reservoir; generating an injection scheme by adopting a physical simulation method; wherein the injection scheme comprises the injection amount and the injection concentration of anaerobic digestion methanogens and substrates of a target oil reservoir; according to the injection scheme, the anaerobic digestion methanogens and the substrate are injected into the target oil reservoir in combination with the well pattern, anaerobic digestion is carried out in the oxygen-free environment, and the biogas is obtained. The natural temperature, mineralization degree, anaerobic environment and other environments of the waste oil reservoir are utilized, substrates and florae are fully stirred and injected into the waste oil reservoir, and then anaerobic digestion is carried out underground through bacteria in an anaerobic environment to generate methane gas.

A microbial culture tank (10) comprises: a riser tank (11) having a structure for raising a culture liquid (S) that cultures methane-producing bacteria that generate methane (M); a downcomer tank (12) having a structure for lowering the culture liquid (S) overflowing from the riser tank (11); and a recovery section (13) for recovering methane (M) released from the culture liquid (S) in the riser tank (11) and the downcomer tank (12).

There is a need to develop alternative and / or improved compositions for reducing methane emissions from animals such as ruminants and / or for increasing, for example, milk production and / or weight gain of such animals by increasing the availability of nutrients to the animals. 【Solution means】Compositions such as animal feed compositions are described for reducing methane emissions and / or for inhibiting one or more methanogenic organisms (i.e., methanogens). The use of such compositions for reducing methane emissions from animals such as ruminants, for inhibiting one or more methanogeneses in said animals, and / or for increasing milk production and / or weight gain of said animals, as well as related methods, are described.

Provided are: a method for producing methane using methanogens, said method having continuously excellent methane production ability even in oxygen (O2) mixing conditions; and a control device and a methane production system that can be used in the production method. Comprising a step of co-culturing methanogens and facultative anaerobic bacteria to produce methane, and a control device and a methane production system that can be used in the production method.

The invention discloses an MEC reactor for promoting sludge deamination and strengthening anaerobic digestion capability by coupling oxides, and relates to the field of anaerobic digestion treatment of sludge in a sewage treatment plant and resource utilization of biogas, the MEC reactor comprises an MEC reactor, an oxidation-reduction potential detection device, a hydrogen detection device, a PLC control module and an oxide adding device; the oxide adding device takes air as an oxide, the air is converted into micro-nano bubbles through a microporous aerator, and ammonia nitrogen is oxidized into nitrate nitrogen; oRP and hydrogen partial pressure in the MEC reactor are monitored in real time through the oxidation-reduction potential detection device and the hydrogen detection device respectively and transmitted to the PLC control module, and the ORP and the hydrogen partial pressure in the MEC reactor main body are intelligently and accurately controlled according to a negative feedback regulation mechanism. The MEC reactor disclosed by the invention relieves the inhibition effect of partial pressure of ammonia nitrogen and hydrogen on methanogens while deaminizing the sludge, improves the biogas yield and strengthens the anaerobic digestion capability of the MEC reactor.

The application discloses a kind of microbial oil extraction methods using green plants as raw material, comprising the following steps: (1) target reservoir screening;(2) target reservoir endogenous microbial community structure analysis;(3) target reservoir methanogen analysis;(4) green plant injection preparation;(5) green plant injection on-site injection, the application is applicable to the field of microbial oil extraction technology, for the reservoir that complete flora exists in endogenous microorganism, on the one hand, carbon fixation is used to green plants, and the emission of carbon dioxide is reduced;On the other hand, endogenous microorganism in reservoir uses green plant injection as nutrition, and acid and gas are produced after degradation, reduce the cost of microbial oil extraction technology, greatly improve the effect of microbial oil extraction;For the reservoir without complete flora, by supplementing functional bacteria, various complex organic matter can be hydrolyzed into relatively simple organic matter, and anaerobic fermentation is carried out to produce gas, effectively improve the recovery efficiency of crude oil, improve the development effect of middle and late reservoir.

The invention discloses a tooth-benefiting xylitol tablet candy and a preparation method thereof. The tooth-benefiting xylitol tablet candy is prepared from the following raw materials: xylitol, modified sodium alginate, HPMCP-genipin, microcrystalline cellulose, colloidal silicon dioxide and magnesium stearate. After the HPMCP-genipin enters the enteric cavity, the shell is dissolved, the L-glutamine and the anhydrous dextrose maintain and stabilize the osmotic pressure of intestinal cells, the genipin of the inner core and the primary amine group on the molecular chain of the modified sodium alginate are covalently crosslinked to form a gel network to wrap the xylitol, and meanwhile, the gel network absorbs water to form a local isotonic environment. A gel network wrapping xylitol enters a large intestine and is dissolved under the action of coliform bacteria, and galactooligosaccharide selectively stimulates the growth of bacteria methanogens capable of utilizing hydrogen, so that the total volume and partial pressure of gas in the intestinal tract are reduced. The microcrystalline cellulose improves the viscous component of the modified sodium alginate, and the colloidal silicon dioxide reduces the shearing and extrusion on the HPMCP-genipin in the tabletting process.

The invention relates to the technical field of sewage treatment, and particularly discloses a domestic sewage advanced treatment process which comprises the following steps: mechanical grating impurity removal, balancing, integrated air floatation, anaerobic treatment, aerobic treatment, precipitation and biological adsorption. Wherein in the anaerobic treatment step, the hydraulic retention of an anaerobic tank is 8-10 hours; the microorganism concentration of anaerobic compound bacteria in the anaerobic tank is 3-6g / L; the anaerobic compound bacteria are a mixture of clostridium butyricum, bifidobacterium longum and methanogens; furthermore, the weight ratio of the clostridium butyricum to the bifidobacterium longum to the methanogens is 1: (0.4-0.6): (1.8-2.3). According to the domestic sewage advanced treatment process provided by the invention, refractory organic matters, suspended solids and ammonia nitrogen in domestic sewage can be effectively removed, so that the effluent quality is stable, and higher discharge and reuse standards can be reached.

The invention discloses modified biochar as well as a preparation method and application thereof, and belongs to the technical field of biochar materials. The preparation method of the modified biochar comprises the following steps: performing ultrasonic treatment on a mixed solution of biochar and a ferric iron solution, then adjusting the pH value of the mixed solution to 10.5-11, and enabling iron adsorbed by the biochar to form ferric hydroxide; washing the biochar adsorbed with the ferric hydroxide until the pH value of a washing solution is 6.5-7.5, and then drying the biochar adsorbed with the ferric hydroxide. The modified biochar prepared by the method can macroscopically improve the hydrolytic acidification rate and methane production efficiency, microscopically enrich hydrolytic flora and methane production flora, participate in electron transfer in biochemical reaction and accelerate material mass transfer, and can be popularized and applied as a kitchen waste anaerobic digestion technology for efficiently producing methane.

The invention discloses a bypass type two-phase anaerobic process and system for kitchen waste treatment and a kitchen waste treatment plant comprising the system, and relates to the technical field of kitchen waste treatment.The bypass type two-phase anaerobic process comprises the following steps that organic slurry obtained through kitchen waste pretreatment is fed into an adjusting tank to be stored; the organic slurry is fed into a hydrolytic acidification tank, macromolecular organic matters in the organic slurry are converted into micromolecular matters by hydrolytic bacteria in the hydrolytic acidification tank, and the micromolecular matters are converted into organic acid, hydrogen and carbon dioxide by acidizing bacteria; the organic slurry subjected to hydrolytic acidification is fed into an anaerobic reaction tank, and organic acid in the organic slurry is converted into methane through methanogens in the anaerobic reaction tank; when the pH in the anaerobic reaction tank is 1t; 6.5 or VFAgt, or VFAgt; when the concentration is 3000 mg / L, the organic slurry subjected to hydrolytic acidification flows back to the regulating tank. According to the invention, the amount of slurry entering the anaerobic reaction tank can be flexibly controlled, the feeding load is balanced, and the load impact of the anaerobic reaction tank is effectively buffered, so that the retention time of the anaerobic reactor can be prolonged.

The application discloses an anaerobic treatment system and process suitable for wastewater containing high organic suspended matter, which comprises a water inlet assembly, a reactor, an internal circulation assembly, a water collecting and discharging assembly and an exhaust assembly; a group of metal membrane tubes are arranged in the reactor, and hydrolytic acid-producing bacteria are loaded in the metal membrane tubes; methanogenic bacteria are filled in the space outside the metal membrane tubes in the reactor; the reactor is connected with the water inlet assembly through the bottom of the metal membrane tubes; the internal circulation assembly is connected with the upper and lower parts of the reactor respectively; the water collecting and discharging assembly is arranged on the top of the reactor, and the reaction water is discharged from the reactor through a discharging pipeline; and the exhaust assembly is connected with the top of the reactor. The application can realize the effect that the acid-producing phase and the methanogenic phase are separated from each other and cooperate with each other, the effluent water is filtered through a membrane, and stable three-phase separation is realized. The application has the advantages of high anaerobic reaction efficiency, stable effluent water quality and high methane yield, and is especially suitable for anaerobic treatment of wastewater containing high organic suspended matter.

The present invention relates to a high-value CO2 gas conversion device based on micro-high-voltage electrochemical technology. The device includes a heating device, a sealed housing, a working electrode, a counter electrode, a reference electrode, and a stirring device. The heating device is arranged to surround the sealed housing. The sealed housing is provided with a cathode chamber and an anode chamber. The cathode chamber contains homoacetogenic bacteria or methanogenic bacteria. The working electrode and the reference electrode extend from the outside of the top cover of the sealed housing into the cathode chamber. The counter electrode extends from the outside of the top cover of the sealed housing into the anode chamber. The stirring device extends from the outside of the top cover of the sealed housing into the cathode chamber and the anode chamber, respectively. The top cover of the sealed housing is provided with liquid inlets and gas inlets and outlets, which are connected to the cathode chamber and the anode chamber. The device uses water and CO2 / CO as raw materials to produce high-value chemicals through microbial electrosynthesis.

The invention relates to the technical field of biomass energy clean conversion, in particular to a power generation system and method based on biomass enzyme catalytic gasification coupling waste recycling, and the system comprises a low-temperature catalytic gasification module, an efficient power generation module and a full-amount recycling module. The method comprises the following steps: catalyzing cellulose and hemicellulose in straws to be decomposed into glucose and xylose in a low-temperature environment and under a micro-positive pressure condition by utilizing a compound preparation consisting of cellulase, ligninase and methanogens, and carrying out anaerobic fermentation to generate methane-containing biogas; biogas is subjected to desulfurization and dehydration pretreatment and then directly generates power through a proton exchange membrane fuel cell, gasification residues are synchronously and completely converted into compound fertilizer through the processes of neutralization, filter pressing, granulation and the like, the comprehensive utilization rate of energy is increased by 50% compared with that of traditional incineration power generation, and a single electric energy output mode is changed.

The present invention provides a method for hydrolyzing and acidifying agricultural waste and a method for synthesizing medium-chain fatty acids, belonging to the technical field of resource utilization of agricultural waste. The present invention provides a method for hydrolyzing and acidifying agricultural waste, comprising: adjusting the solid content of the agricultural waste, mixing it with short-chain AHLs and chitosan microspheres loaded with DPD to obtain a hydrolysis and acidification system; and subjecting the hydrolysis and acidification system to anaerobic fermentation to obtain a hydrolysis and acidification product. This method adds short-chain AHLs during the hydrolysis and acidification process of agricultural waste to activate genes related to hydrolysis and acidification bacteria, promote biofilm formation and extracellular enzyme secretion, improve substrate hydrolysis and acidification efficiency, and promote substrate decomposition and short-chain fatty acid accumulation; DPD is delayed in release during the metabolic peak of acid-producing bacteria, coordinating the metabolic division of hydrolytic bacteria and acid-producing bacteria, inhibiting the activity of methanogens, reducing substrate competition, improving the degradation rate of agricultural waste cellulose, and increasing the yield of medium-chain fatty acid precursors such as acetic acid in the fermentation substrate.

The present application relates to wastewater resource treatment technical field, especially to a kind of magnetic field reinforced anaerobic reactor and wastewater resource treatment method.The magnetic field reinforced anaerobic reactor provided by the present application, including anaerobic reactor body and multiple magnetic blocks fixed on the outer surface of the anaerobic reactor body, wherein the multiple magnetic blocks are divided into multiple rows and circled on the outer surface of the anaerobic reactor body, and the magnetic blocks in each row are arranged in the order of left, upper, right and down according to the direction of magnetic field.The magnetic field reinforced anaerobic reactor of the present application can convert the movement of microorganisms in the reactor relying on water flow, bubble or itself rising or falling into cutting magnetic induction line movement by using magnetic field, convert kinetic energy into potential energy, enhance the electron transfer between acetogenic bacteria and methanogens, improve the utilization of organic matter in wastewater by acid-producing bacteria and the process of methanogens utilizing electron to reduce carbon dioxide to produce methane, and realize the improvement of energy utilization and methanogenesis efficiency in anaerobic reactor.