147 results about "Methanobacter" patented technology

An in-situ biogas purification method for anaerobic digestion of sludge based on exogenous hydrogen supply relates to the field of solid waste resource utilization and renewable energy technology. The purpose of the present invention is to solve the problems of low hydrogen solubility and insufficient carbon dioxide bioconversion in the biogas purification process, as well as the inhibitory effect of excessive hydrogen dosage on biogas production by anaerobic digestion of sludge. The present invention utilizes the anaerobic digestion process of sludge in an anaerobic environment to produce biogas, and adds hydrogen to the process by direct hydrogen input or zero-valent iron corrosion, so that hydrogen penetrates into the liquid phase. The hydrogenotrophic methanogens in the system use hydrogen as an electron donor and carbon dioxide in the biogas produced by anaerobic digestion of sludge as an electron acceptor to convert hydrogen and carbon dioxide into methane to achieve in-situ biogas purification. The present invention can obtain an in-situ biogas purification method for anaerobic digestion of sludge based on exogenous hydrogen supply.

The invention provides an inoculum domestication method for relieving acid or ammonia nitrogen inhibition of an anaerobic fermentation system, which comprises the following steps of: carrying out constant-temperature anaerobic fermentation culture at medium temperature (30-35 DEG C) or high temperature (50-55 DEG C) until the gas yield is stable by taking livestock manure, activated sludge, industrial anaerobic digestion residues or mineralized refuse biogas fermentation liquor and the like as inoculums; the obtained inoculum is an inoculum which has relatively strong acid tolerance or ammonia nitrogen tolerance after domestication. The domesticated inoculum with good tolerance and the original untreated inoculum are mixed according to a certain proportion and then put into an anaerobic fermentation system, and wet fermentation (TS = 5-10% and total solid content) or dry fermentation (TS = 20-30%) is carried out, so that the pH value of the anaerobic fermentation system is recovered to a state suitable for growth of methanogens.

The invention discloses methanogen lyase homologous with high-dimensional characteristics of PeiR lyase and application of the methanogen lyase and aims to solve the problems that PeiR lyase protein of methanogen cell wall peptide bonds can participate in the biological process of hydrolyzing archaea cell walls, methanogen is effectively killed, methane production is reduced, and protein homologous with the protein has potential methane reduction potential. According to the invention, a series of biological information software is utilized to identify protein sequences coded by methanogen viruses from a large amount of rumen microbiome sequencing data, and high-dimensional homology between the proteins and PeiR proteins based on a deep learning model TM-Vec is further analyzed, so that a series of methanogen lyases are obtained. In order to realize the expression of the lyase, multiple segments of primers are designed to synthesize a target sequence, and the target sequence is successfully expressed in a prokaryotic expression system. Through the detection of crude enzyme liquid, the effectiveness of the lyase in the aspect of reducing the methane yield is verified in an in-vitro gas production test.

To provide a methanation system which has good efficiency of conversion of carbon dioxide to methane even when gas containing a low concentration of carbon dioxide is used as a carbon dioxide source, and which enables simplification of equipment and reduction of installation area thereof.SOLUTION: Methanation equipment is provided, comprising: a dissolution tank exposing carbon dioxide-containing gas to an aqueous phase, thereby producing carbon dioxide-containing solution; a culture tank for culturing methane in culture solution, thereby producing methane; a carbon dioxide gas supply flow passage for introducing carbon dioxide-containing gas into the dissolution tank; solution transfer means transferring the carbon dioxide-containing solution produced in the dissolution tank from the dissolution tank to the culture tank; a hydrogen supply flow passage for supplying hydrogen to the dissolution tank or the culture tank; and a methane discharge flow passage for discharging the produced methane from the culture tank.SELECTED DRAWING: Figure 1

The invention discloses a micro-ecological flora in-situ screening and anaerobic acid production enrichment method and device based on selective metal ion removal, belongs to the field of anaerobic treatment of high-concentration organic wastes, and solves the problems of coordinating the flora relationship of the organic wastes and improving the enrichment efficiency of anaerobic produced short-chain fatty acids. The method comprises the following steps: gradually removing soluble Fe, Co, Ni, Mg and Ca in an exchangeable state and a carbonate binding state from organic wastes by using a filler loaded with chelating groups, creating a multivalent metal ion deficiency environment, and stresses the evolution of a micro-ecosystem by using the selectivity dependence difference of acid-producing fermentation flora and methanogenic flora on metal ions, so as to achieve the purpose of improving the yield of the organic wastes. The interaction relationship between the multivalent metal and the acidogenic fermentation flora and methanogenic flora is regulated and controlled, the flora screening effect is exerted, the acidogenic fermentation flora is enriched, the methanogenic flora is inhibited, and the acidogenic fermentation flora is promoted to become dominant bacteria for mass propagation and proliferation, so that the acidogenic reaction is improved and the methanogenic reaction is inhibited; the efficiency improvement and the lossless enrichment of anaerobic acid production of the high-concentration organic waste are realized.

The invention discloses a method for improving biogas biological purification efficiency based on a nanometer zero-valent iron composite material, which comprises the following steps: adding the nanometer zero-valent iron composite material into kitchen wastewater, adding anaerobic granular sludge into a mixed system, keeping the pH value of the whole reaction system at 7.2 + / -0.2, blowing off for 5 minutes by using nitrogen to keep an anaerobic environment, and carrying out solid-liquid separation to obtain the biogas biological purification efficiency. The generated biogas is collected by a gas collecting bag; wherein the nano zero-valent iron composite material comprises nano zero-valent iron coated with an extracellular polymer and / or nano zero-valent iron coated with lauryl sodium sulfate. Methane is produced through anaerobic fermentation of kitchen wastewater, the nano zero-valent iron composite material is added into an anaerobic system, H2 is provided for hydrogen-philic methanogens through corrosion and hydrogen evolution of the nano zero-valent iron composite material, and the heat value of biogas is further increased.

Compositions, such as animal feed compositions, for reducing methane emissions and / or for inhibiting one or more methanogens (i.e. Methanogens) are described. The use of the compositions for reducing methane emissions from an animal, such as a ruminant, for inhibiting methane production by one or more methanogens in the animal, and / or for increasing milk yield and / or weight gain in the animal, and related methods are described.

The invention discloses a humic acid capacitor for stimulating microbial electrical metabolism and application of the humic acid capacitor in anaerobic digestion, and belongs to the field of biological metabolism and transformation application. The electron storage capacity of humic acid is improved through micro-current catalysis, so that the humic acid becomes a biological capacitor capable of promoting microbial electrical metabolism. The biological capacitor can be used for adjusting unbalanced metabolism between acid-producing bacteria and methanogens in the sludge anaerobic digestion process, the electron utilization rate in a biological metabolism and conversion system is improved, and the methane production performance of sludge anaerobic digestion is greatly improved; the humic acid capacitor can increase the daily methane yield in the sludge anaerobic digestion process by 80-100%. According to the method, the biological capacitor with excellent performance can be obtained only by applying the micro current, and the method is green, environment-friendly, low in cost and high in efficiency, is a new method and idea for improving the anaerobic digestion performance of the sludge, can be popularized to the fields of development and utilization of new energy such as other biological metabolism and conversion and solar energy, and has huge application value.

The invention provides an intelligent optimization method for carbon emission reduction of an ecological pasture in extreme weather, and relates to the technical field of intelligent optimization of carbon emission reduction. Comprising the steps that initial monitoring data are collected and preprocessed, and multi-dimensional feature vector output state features are constructed; an LSTM time sequence prediction model is used for analyzing the state of the dairy cow and the activity of methanogens in the rumen, DHI data of an integrated gradient boosting tree model is used for analyzing the amino acid balance and precise nutrition technology of the lactating dairy cow, a dairy cow manure yield prediction model based on a Bayesian network is constructed, a manure management strategy is dynamically optimized, and manure management effect data is output. And constructing a carbon emission reduction comprehensive evaluation model, and evaluating the pasture carbon emission reduction effect. According to the invention, the rumen flora structure is regulated and controlled, the rumen greenhouse gas emission is controlled, the ruminant feed protein level is reduced, and the methane emission is reduced by researching the dairy cow carbon emission rumen microorganism regulation and control technology.

The invention discloses a hydrolytic acidification-AO combined treatment device and method for printing and dyeing wastewater, the device comprises a hydrolytic acidification tank, an AO tank and a sedimentation tank, the hydrolytic acidification tank is internally provided with an iron-carbon micro-electrolysis filler used for enhancing pollutant degradation and inhibiting methanogens growth, the AO tank comprises an anoxic tank and an aerobic tank, and the anoxic tank is provided with an aerobic tank. The sedimentation tank is internally provided with a conveying device for respectively refluxing mud-water mixed liquid and nitrification liquid to the hydrolysis acidification tank and the anoxic tank; the preparation method of the iron-carbon micro-electrolysis filler comprises the following steps: S1, uniformly mixing zero-valent iron powder, powdered activated carbon and kaolin; s2, sodium bicarbonate is added into the mixture, and then water is added for stirring; s3, preparing granular filler balls from the mixture, drying the filler balls, and performing anaerobic calcination; s4, manganese sulfate monohydrate is added firstly, then water is added, a mixed solution is obtained, a potassium permanganate solution is added, stirring is performed, and standing and aging are performed; and S5, drying the filler balls. The device can enhance the pollutant degradation effect and reduce carbon emission at the same time.

The present invention belongs to the technical field of agricultural waste resource utilization, and specifically relates to a method for strengthening high-solid content anaerobic fermentation by micro-aerobic aeration. The present invention provides a method for strengthening high-solid content anaerobic fermentation. The technical scheme of the present invention improves the utilization efficiency of all components of agricultural waste and improves the gas production rate by performing micro-aerobic aeration on the 1st to 3rd day and / or the 12th to 14th day of anaerobic fermentation. Magnetic porous microspheres (MPMs) are added to enrich efficient methanogens by utilizing the porous characteristics of MPMs, and the electron transfer capacity of the solid-liquid interface is improved by layered inoculation of inoculum and external application of weak magnetic field and weak electric field, thereby improving the degradation of lignocellulose and the efficiency of methanogenesis. Adding biological lipopeptide surfactants in the leachate reflux process can promote the generation of organic acids, increase the activity of coenzyme F420, a key enzyme for methanogenesis, promote the conversion of intermediate products, and improve the biodegradability and gas production rate of agricultural waste.

The invention belongs to the technical field of sewage treatment, and provides a method for relieving inhibition of polyvinyl chloride micro-plastics on an anaerobic digestion process by using sludge and coal gangue based biochar, which comprises the following steps: adding pretreated sludge and simulated wastewater into an SBR (Sequencing Batch Reactor); soaking PVC-MPs in alcohol for 48 hours, washing the PVC-MPs with distilled water for 3-5 times to remove impurities in the PVC-MPs, and drying the PVC-MPs at 105 DEG C for 24 hours for later use; pVC-MPs is added into a reaction device according to the proportion of 0.1 g. (g. VS)-1 and placed in a constant-temperature shaking table at the temperature of 35.0 + / -0.1 DEG C in a dark place; sCBC is added into the reaction device according to the adding amount of 1.0 g.L <-1 >, other conditions are kept the same, and sampling and analysis are conducted at 0, 1, 2, 3, 5, 7, 9 and 12 h respectively. The invention proves that the SCBC can effectively relieve the inhibition of PVC-MPs on anaerobic digestion, promote the enrichment of dominant methanogens, and provide a feasible technical path for the resource utilization of coal gangue and sludge at the same time.

The invention relates to the technical field of wastewater resourceful treatment, in particular to a magnetic field reinforced anaerobic reactor and a wastewater resourceful treatment method. The magnetic field intensified anaerobic reactor comprises an anaerobic reactor body and a plurality of magnetic blocks fixed on the outer surface of the anaerobic reactor body, the plurality of magnetic blocks are divided into a plurality of rows and surround the outer surface of the anaerobic reactor body, and the magnetic blocks in each row are circularly arranged in the sequence of left, upper, right and lower according to the magnetic field direction. The magnetic field enhanced anaerobic reactor can utilize the magnetic field to convert the ascending or descending motion of microorganisms in the reactor depending on water flow, bubbles or self into magnetic induction line cutting motion, convert kinetic energy into electric potential energy and enhance electron transfer between acetogens and methanogens; the utilization degree of organic matters in the wastewater by the acid-producing bacteria and the methane-producing process of reducing carbon dioxide by utilizing electrons by the methane-producing bacteria are improved, and the energy utilization and methane-producing efficiency in the anaerobic reactor are improved.

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.

The utility model belongs to sewage treatment technical field, specifically is a kind of sludge reduction's sewage treatment equipment, including base, the base top is fixed with processing cylinder, the processing cylinder bottom is fixed with bottom plate, by setting movable cylinder, second movable shaft, stirring plate, stirring rod and stirring ring, can make sludge particle in sewage even suspended, let hydrolytic bacteria, methanogen etc. and the organic matter in sludge fully contact, accelerate hydrolysis, acidification and the rate of methanogenesis reaction, reduce substrate residue, and anaerobic sludge is kept for a long time, can form dense precipitate layer in processing cylinder bottom, lead to effective reaction volume shrinkage, stirring can make sludge keep suspended state, maintain the effective volume utilization rate of processing cylinder, while anaerobic treatment needs stable temperature, if not stirring, local temperature difference in processing cylinder is easy to appear, lead to microbial activity imbalance, by stirring can maintain the uniformity of sewage anaerobic environment, stable reaction condition.

The invention provides an agricultural waste hydrolytic acidification method and a method for synthesizing medium-chain fatty acid, and belongs to the technical field of agricultural waste resource utilization. The invention provides an agricultural waste hydrolytic acidification method which comprises the following steps: after adjusting the solid content of agricultural waste, mixing the agricultural waste with short-chain AHLs and DPD-loaded chitosan microspheres to obtain a hydrolytic acidification system; and carrying out anaerobic fermentation on the hydrolytic acidification system to obtain a hydrolytic acidification product. According to the method, short-chain AHLs are added in the hydrolytic acidification process of the agricultural wastes to activate related genes of hydrolytic acidification bacteria, so that biofilm formation and extracellular enzyme secretion are promoted, the hydrolytic acidification efficiency of a substrate is improved, and substrate decomposition and short-chain fatty acid accumulation are promoted; dPD is released in a delayed manner in the metabolic peak period of acid-producing bacteria, the metabolic division of hydrolytic bacteria and acid-producing bacteria is coordinated, the activity of methanogens is inhibited, substrate competition is reduced, the degradation rate of agricultural waste cellulose is increased, and the yield of medium-chain fatty acid precursor substances such as acetic acid in fermentation substrates is increased.

The present application relates to the technical field of excrement treatment, and particularly relates to a feces treatment method based on flora analysis, comprising obtaining reaction temperature and material Ph value and determining gas production relative deviation based on gas production, determining device operation state based on characteristic parameters; in response to the device operation state, determining abnormal characteristic identification to obtain key functional flora parameters, determining flora balance index based on the key functional flora parameters to determine whether acid-producing bacteria and methanogenic bacteria are in flora balance; determining smoothness index according to the change curve of a plurality of actual flora balance indexes obtained in an initial detection period to determine the deviation degree of the flora balance index, and adjusting the correction sensitivity coefficient of the feed adjustment amount based on the smoothness index. The present application performs real-time monitoring and dynamic regulation on the excrement treatment process based on the flora analysis result to maintain optimal flora structure and metabolic activity, and improves treatment efficiency and stability.

The invention provides a material and a method for cleaning biogas residues produced by anaerobic digestion of waste oil reservoirs. The method comprises the following steps: (1) determining required anaerobic digestion methanogens and substrates according to the types of biogas residues produced by anaerobic digestion of the waste oil reservoir; (2) the oil reservoir is screened according to the requirements of the anaerobic digestion methanogens and the substrate for temperature, mineral substances, pH value and permeability, and a standard rock core is obtained; (3) adopting a physical simulation method, injecting anaerobic digestion methanogens and different organic matters into the standard rock core to clean in a manner of producing biogas residues through anaerobic digestion, and performing biogas residue cleaning test on the biogas residues generated by the different organic matters to obtain a cleaning scheme; and (4) cleaning the biogas residues produced by anaerobic digestion of the waste oil reservoir according to the cleaning scheme. A classification cleaning strategy is designed according to the blocking risk of organic matter biogas residues of different sources, the method for preparing the biogas through anaerobic digestion of the waste oil reservoir can be achieved, and the in-situ biogas preparation efficiency is improved.

The invention discloses a binary pseudocapacitance composite material for promoting anaerobic digestion of kitchen waste and a preparation method and application thereof, and belongs to the technical field of organic solid waste recycling and renewable energy sources. The wood chip biochar-polydopamine-Fe3O4 composite material with a core-shell structure is constructed by taking wood chips as raw materials, preparing biochar through low-temperature pyrolysis, forming an interface modification layer through polydopamine coating, loading Fe < 3 + > and carrying out secondary pyrolysis to generate Fe3O4 nanoparticles (40-110nm). The material can be recovered through a magnetic field (the recovery rate is greater than or equal to 90%), when the dosage is 0.8-1.2 g / L, the peak value of volatile fatty acid in an anaerobic digestion system is obviously reduced to be less than or equal to 400 mg / L, the methane accumulation yield is improved to be greater than or equal to 45%, the electron transfer efficiency of acid-producing bacteria and methanogens is synchronously optimized, and the system operation is stabilized.

The application provides a method for enhancing anaerobic digestion of organic solid waste containing new pollutant antibiotics by circulating oxygen disturbance, and belongs to the technical field of solid waste resource treatment. The method introduces circulating and increasing micro-nano bubble oxygen water stress during the stable operation period of anaerobic digestion, uses the dynamic change of physical oxidation pressure to induce the adversity defense mechanism of microorganisms, and promotes the improvement of metabolic activity and optimization of community structure of functional flora in situ. By accurately regulating the key process parameters such as oxygen disturbance cycle, gradient dosing ratio and the like, the application not only avoids the excessive inhibition of oxygen on methanogens, but also significantly enhances the adaptability of the system to the environmental stress of new pollutants. The application has the advantages of no need for external agents, high physical mass transfer efficiency, significant yield increase and harmless effect, and is suitable for in-situ strengthening and deep performance improvement of anaerobic digestion systems of municipal sludge, livestock and poultry manure and kitchen garbage and the like.

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.

The invention discloses a method for rapidly constructing denitrification type anaerobic methane oxidation through hydrogen type methanogens, and relates to the technical field of sewage treatment. According to the method, anaerobic digestion sludge of a sewage treatment plant is taken for inoculation, and a methanogen-mediated denitrification type anaerobic methane oxidation process is constructed. Hydrogen-type methanogens in the anaerobic digestion system excite a reverse methanogenesis function under the condition that organic matters are deficient, and denitrification anaerobic methane oxidation is realized by coupling sulfate and nitrate with nitrogen and sulfur circulating microorganisms. Sulfate is used as a potential electron acceptor in sewage, and dissoluble methane is removed while sulfate is indirectly utilized through recessive biological sulfur cycle catalytic bridging anaerobic methane oxidation and denitrification. In addition, the anaerobic digestion sludge can be continuously inoculated with hydrogen-type methanogens, and compared with traditional denitrification type anaerobic methane oxidation archaea culture, the method is more convenient. Therefore, the denitrifying anaerobic methane oxidation rapidly constructed by the hydrogen-type methanogens is an effective measure for breaking through the deep denitrification of the sewage treatment plant and synchronously controlling and discharging the greenhouse gas.

The present invention discloses a method for enhancing methane production and carbon sequestration in anaerobic digestion by zero-valent iron in cooperation with exogenous CO2. The method includes: inoculating sludge in a reactor, adding zero-valent iron thereto, and then adding wastewater. The reaction temperature is maintained at 35 ± 1 °C, and CO2 is injected into the liquid surface at a flow rate of 1.5 L / min for 5 - 50 min / L under stirring. CO2 and zero-valent iron jointly regulate the pH of the system, providing a suitable anaerobic digestion environment for microorganisms. Through the corrosion reaction of zero-valent iron under anaerobic conditions, electrons are released and H2 is generated, stimulating the proliferation of hydrogen-consuming microorganisms, while improving the activity of microorganisms and enzymes, increasing the methane production and rate. In addition, some methanogens in the system utilize H2 and CO2 to produce amorphous carbon, and zero-valent iron and exogenous CO2 are used to regulate the growth of amorphous carbon, strengthening the fixation of CO2 in the system.

The present invention provides a method for producing methane by dry anaerobic fermentation, which relates to the technical field of anaerobic fermentation. In the present invention, a biochar-immobilized complex enzyme is added during the dry anaerobic fermentation process. The biochar-immobilized complex enzyme is achieved by fixing the enzyme to biochar, which can enhance the stability and resistance to protein degradation of the enzyme, extend the service life of the enzyme, and reduce the inactivation rate of the enzyme, thereby facilitating an increase in methane production. In addition, biochar has a high specific surface area and the characteristics of promoting interspecies electron transfer, which is conducive to increasing methane production. Moreover, in the biochar-immobilized complex enzyme, biochar fixes the enzyme on its surface or interior, which can provide more reactive sites, which is conducive to the enrichment of methanogens, thereby shortening the fermentation reaction cycle and facilitating an increase in the reaction rate of anaerobic fermentation. In summary, the reaction rate and methane yield of the method for producing methane by dry anaerobic fermentation provided by the present invention are both high.

The invention discloses an emergency-adjustable anti-ammonia inhibition anaerobic system and an emergency adjustment method, comprising: the inlet end of the hydrolysis acidification reactor is connected to the organic slurry conveying pipeline, and the outlet end thereof is connected to the anaerobic digestion reactor; the anaerobic digestion reactor is connected to the agent dosing device through the agent conveying pipeline with an electromagnetic opening and closing valve, and an online ammonia concentration detector and an online pressure detector are installed on the anaerobic digestion reactor; the outlet end of the flushing water collection tank is connected to the flushing water pipeline, the flushing water pipeline is connected to the organic slurry conveying pipeline, and the data output ends of the online ammonia concentration detector and the online pressure detector are connected to the controller; the controller controls the opening and closing of the agent conveying pipeline according to the data processing result. The invention not only adopts multiple anti-ammonia inhibition measures, thereby preventing the inhibition of methanogens by free ammonia in the anaerobic digestion reactor, but also avoids the influence of abnormal conditions on the operation of the system through an emergency adjustment mechanism, thereby significantly improving the economic benefits of the food waste treatment plant.

The invention relates to the technical field of biological fermentation engineering, and discloses a method for preparing clean biological methane gas from duckweed residues, which comprises the following steps: fermenting anaerobic granular sludge as an inoculant and duckweed residues as a fermentation substrate in an anaerobic fermentation tank to prepare methane; the anaerobic fermentation tank comprises a tank body, a cover body and a regulation and control unit, wherein the regulation and control unit comprises a controller, a display screen, a barrel, a telescopic device for driving the barrel to lift, and a pH sensor, a conductivity sensor and an acid and alkali liquor adding pipe which are fixed in the barrel; the liquid inlet end of the acid and alkali liquid adding pipe is communicated with an acid liquid pipe, an alkali liquid pipe and a flushing liquid pipe through a four-way valve; the telescopic device, the pH sensor, the conductivity sensor, the four-way valve and the display screen are all electrically connected with the controller. According to the scheme, the regulation and control unit is additionally arranged, so that the pH of the fermentation liquor is effectively and accurately regulated and controlled in real time, the pH of the fermentation liquor is ensured to be within the optimal active pH range of methanogens, the activity of the methanogens and the methane yield are further improved, and the production benefits are improved.

The invention provides a method for preparing biogas by anaerobic digestion of a waste oil reservoir, which comprises the following steps of: determining required anaerobic digestion methanogens and substrates according to geological characteristics, temperature, salinity and residual oil components of a target waste oil reservoir; the abandoned oil reservoirs are screened through the geological parameters and the well pattern mode, and target oil reservoirs are obtained; arranging a well pattern form in which vertical wells and horizontal wells are combined on a target oil reservoir; generating an injection scheme by adopting a physical simulation method; wherein the injection scheme comprises the injection amount and the injection concentration of anaerobic digestion methanogens and substrates of a target oil reservoir; according to the injection scheme, the anaerobic digestion methanogens and the substrate are injected into the target oil reservoir in combination with the well pattern, anaerobic digestion is carried out in the oxygen-free environment, and the biogas is obtained. The natural temperature, mineralization degree, anaerobic environment and other environments of the waste oil reservoir are utilized, substrates and florae are fully stirred and injected into the waste oil reservoir, and then anaerobic digestion is carried out underground through bacteria in an anaerobic environment to generate methane gas.

A microbial culture tank (10) comprises: a riser tank (11) having a structure for raising a culture liquid (S) that cultures methane-producing bacteria that generate methane (M); a downcomer tank (12) having a structure for lowering the culture liquid (S) overflowing from the riser tank (11); and a recovery section (13) for recovering methane (M) released from the culture liquid (S) in the riser tank (11) and the downcomer tank (12).

The invention relates to the field of feed technology, and specifically discloses a Schizochytrium powder for reducing methane emission of dairy cows and a preparation method thereof, as well as a double-layer microalgae whole-cell Schizochytrium powder or a microalgae whole-cell Schizochytrium powder with an inner core of Schizochytrium and an outer double-layer coating structure obtained by the method. Methanogens in the rumen can reduce carbon dioxide to CH4 using hydrogen. The feed formula of the invention adopts a multi-layer core-coating structure, adds lysozyme in the outermost coating of Schizochytrium particles, adds 4-hydroxy-3-methoxybenzoic acid, D-alloisoleucine and D-2-aminobutyric acid in the middle coating to inhibit the growth of Methanobacterium brevis and Methanobacterium, adjusts the flora structure, promotes the metabolism of the flora in the rumen to move to the propionic acid path, reduces the amount of acetic acid and the ratio of acetic acid to propionic acid, promotes digestion, increases the VFA fermentation yield and the amount of propionic acid, thereby reducing methane emission and promoting the absorption of various nutrients of Schizochytrium without affecting the fermentation effect of the flora. Long-term use can significantly increase the content of nutrients such as DHA in milk.

There is a need to develop alternative and / or improved compositions for reducing methane emissions from animals such as ruminants and / or for increasing, for example, milk production and / or weight gain of such animals by increasing the availability of nutrients to the animals. 【Solution means】Compositions such as animal feed compositions are described for reducing methane emissions and / or for inhibiting one or more methanogenic organisms (i.e., methanogens). The use of such compositions for reducing methane emissions from animals such as ruminants, for inhibiting one or more methanogeneses in said animals, and / or for increasing milk production and / or weight gain of said animals, as well as related methods, are described.