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51 results about "Methanobacter" patented technology

Methanobacteria can be used in biomass conversion as well as energy production through Anaerobic digestion (AD) process. Microbial community is used in Anaerobic digestion(AD) to convert organic wastes into clean energy by reducing chemical and biological oxygen demand in the wastes.

Salt-tolerant methanogenic bacteria liquid for treating high-salt steroid pharmaceutical wastewater and preparation method of salt-tolerant methanogenic bacteria liquid

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.
Owner:JIANGNAN UNIV

A feces treatment method based on flora analysis

The present application relates to the technical field of excrement treatment, and particularly relates to a feces treatment method based on flora analysis, comprising obtaining reaction temperature and material Ph value and determining gas production relative deviation based on gas production, determining device operation state based on characteristic parameters; in response to the device operation state, determining abnormal characteristic identification to obtain key functional flora parameters, determining flora balance index based on the key functional flora parameters to determine whether acid-producing bacteria and methanogenic bacteria are in flora balance; determining smoothness index according to the change curve of a plurality of actual flora balance indexes obtained in an initial detection period to determine the deviation degree of the flora balance index, and adjusting the correction sensitivity coefficient of the feed adjustment amount based on the smoothness index. The present application performs real-time monitoring and dynamic regulation on the excrement treatment process based on the flora analysis result to maintain optimal flora structure and metabolic activity, and improves treatment efficiency and stability.
Owner:NINGXIA MEDICAL UNIVERSITY GENERAL HOSPITAL

Binary pseudocapacitance composite material for promoting anaerobic digestion of kitchen waste as well as preparation method and application of binary pseudocapacitance composite material

The invention discloses a binary pseudocapacitance composite material for promoting anaerobic digestion of kitchen waste and a preparation method and application thereof, and belongs to the technical field of organic solid waste recycling and renewable energy sources. The wood chip biochar-polydopamine-Fe3O4 composite material with a core-shell structure is constructed by taking wood chips as raw materials, preparing biochar through low-temperature pyrolysis, forming an interface modification layer through polydopamine coating, loading Fe < 3 + > and carrying out secondary pyrolysis to generate Fe3O4 nanoparticles (40-110nm). The material can be recovered through a magnetic field (the recovery rate is greater than or equal to 90%), when the dosage is 0.8-1.2 g / L, the peak value of volatile fatty acid in an anaerobic digestion system is obviously reduced to be less than or equal to 400 mg / L, the methane accumulation yield is improved to be greater than or equal to 45%, the electron transfer efficiency of acid-producing bacteria and methanogens is synchronously optimized, and the system operation is stabilized.
Owner:SHAANXI UNIV OF SCI & TECH

A method for enhancing anaerobic digestion of organic solid waste containing antibiotic new pollutants by circulating oxygen disturbance and application

The application provides a method for enhancing anaerobic digestion of organic solid waste containing new pollutant antibiotics by circulating oxygen disturbance, and belongs to the technical field of solid waste resource treatment. The method introduces circulating and increasing micro-nano bubble oxygen water stress during the stable operation period of anaerobic digestion, uses the dynamic change of physical oxidation pressure to induce the adversity defense mechanism of microorganisms, and promotes the improvement of metabolic activity and optimization of community structure of functional flora in situ. By accurately regulating the key process parameters such as oxygen disturbance cycle, gradient dosing ratio and the like, the application not only avoids the excessive inhibition of oxygen on methanogens, but also significantly enhances the adaptability of the system to the environmental stress of new pollutants. The application has the advantages of no need for external agents, high physical mass transfer efficiency, significant yield increase and harmless effect, and is suitable for in-situ strengthening and deep performance improvement of anaerobic digestion systems of municipal sludge, livestock and poultry manure and kitchen garbage and the like.
Owner:HUNAN UNIV

Aerobic-anaerobic combined pretreatment of coal gasification device and method

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.
Owner:HENAN POLYTECHNIC UNIV

Method for rapidly constructing denitrification type anaerobic methane oxidation through hydrogen type methanogens

PendingCN121672763AWaste based fuelTreatment with anaerobic digestion processesAnaerobic oxidation of methaneMethanobacter
The invention discloses a method for rapidly constructing denitrification type anaerobic methane oxidation through hydrogen type methanogens, and relates to the technical field of sewage treatment. According to the method, anaerobic digestion sludge of a sewage treatment plant is taken for inoculation, and a methanogen-mediated denitrification type anaerobic methane oxidation process is constructed. Hydrogen-type methanogens in the anaerobic digestion system excite a reverse methanogenesis function under the condition that organic matters are deficient, and denitrification anaerobic methane oxidation is realized by coupling sulfate and nitrate with nitrogen and sulfur circulating microorganisms. Sulfate is used as a potential electron acceptor in sewage, and dissoluble methane is removed while sulfate is indirectly utilized through recessive biological sulfur cycle catalytic bridging anaerobic methane oxidation and denitrification. In addition, the anaerobic digestion sludge can be continuously inoculated with hydrogen-type methanogens, and compared with traditional denitrification type anaerobic methane oxidation archaea culture, the method is more convenient. Therefore, the denitrifying anaerobic methane oxidation rapidly constructed by the hydrogen-type methanogens is an effective measure for breaking through the deep denitrification of the sewage treatment plant and synchronously controlling and discharging the greenhouse gas.
Owner:CHONGQING UNIV

Bypass type two-phase anaerobic process and system for kitchen waste treatment and kitchen waste treatment plant comprising system

The invention discloses a bypass type two-phase anaerobic process and system for kitchen waste treatment and a kitchen waste treatment plant comprising the system, and relates to the technical field of kitchen waste treatment.The bypass type two-phase anaerobic process comprises the following steps that organic slurry obtained through kitchen waste pretreatment is fed into an adjusting tank to be stored; the organic slurry is fed into a hydrolytic acidification tank, macromolecular organic matters in the organic slurry are converted into micromolecular matters by hydrolytic bacteria in the hydrolytic acidification tank, and the micromolecular matters are converted into organic acid, hydrogen and carbon dioxide by acidizing bacteria; the organic slurry subjected to hydrolytic acidification is fed into an anaerobic reaction tank, and organic acid in the organic slurry is converted into methane through methanogens in the anaerobic reaction tank; when the pH in the anaerobic reaction tank is 1t; 6.5 or VFAgt, or VFAgt; when the concentration is 3000 mg / L, the organic slurry subjected to hydrolytic acidification flows back to the regulating tank. According to the invention, the amount of slurry entering the anaerobic reaction tank can be flexibly controlled, the feeding load is balanced, and the load impact of the anaerobic reaction tank is effectively buffered, so that the retention time of the anaerobic reactor can be prolonged.
Owner:CHENGDU XINGRONG RENEWABLE ENERGY CO LTD

Anaerobic treatment system and process suitable for high organic suspended solids containing wastewater

ActiveCN118164612Bavoid competitionAvoid mutualismWater contaminantsWaste based fuelMethanobacterMethane yield
The application discloses an anaerobic treatment system and process suitable for wastewater containing high organic suspended matter, which comprises a water inlet assembly, a reactor, an internal circulation assembly, a water collecting and discharging assembly and an exhaust assembly; a group of metal membrane tubes are arranged in the reactor, and hydrolytic acid-producing bacteria are loaded in the metal membrane tubes; methanogenic bacteria are filled in the space outside the metal membrane tubes in the reactor; the reactor is connected with the water inlet assembly through the bottom of the metal membrane tubes; the internal circulation assembly is connected with the upper and lower parts of the reactor respectively; the water collecting and discharging assembly is arranged on the top of the reactor, and the reaction water is discharged from the reactor through a discharging pipeline; and the exhaust assembly is connected with the top of the reactor. The application can realize the effect that the acid-producing phase and the methanogenic phase are separated from each other and cooperate with each other, the effluent water is filtered through a membrane, and stable three-phase separation is realized. The application has the advantages of high anaerobic reaction efficiency, stable effluent water quality and high methane yield, and is especially suitable for anaerobic treatment of wastewater containing high organic suspended matter.
Owner:NANJING UNIV OF FINANCE & ECONOMICS

Anaerobic reactor with magnetic field reinforcement and wastewater resource treatment method

The present application relates to wastewater resource treatment technical field, especially to a kind of magnetic field reinforced anaerobic reactor and wastewater resource treatment method.The magnetic field reinforced anaerobic reactor provided by the present application, including anaerobic reactor body and multiple magnetic blocks fixed on the outer surface of the anaerobic reactor body, wherein the multiple magnetic blocks are divided into multiple rows and circled on the outer surface of the anaerobic reactor body, and the magnetic blocks in each row are arranged in the order of left, upper, right and down according to the direction of magnetic field.The magnetic field reinforced anaerobic reactor of the present application can convert the movement of microorganisms in the reactor relying on water flow, bubble or itself rising or falling into cutting magnetic induction line movement by using magnetic field, convert kinetic energy into potential energy, enhance the electron transfer between acetogenic bacteria and methanogens, improve the utilization of organic matter in wastewater by acid-producing bacteria and the process of methanogens utilizing electron to reduce carbon dioxide to produce methane, and realize the improvement of energy utilization and methanogenesis efficiency in anaerobic reactor.
Owner:CHINA AGRI UNIV

Device and method for regulating biological membrane niche to realize simultaneous methanogenesis and denitrification

The application provides a device and method for regulating biological membrane niche to realize simultaneous methanogenesis and denitrification, and belongs to the technical field of water treatment, comprising a biological membrane reactor, a water distribution tank, a peristaltic pump, an aeration system and an electric stirrer, the biological membrane reactor is filled with biological membrane carriers, and an anoxic-anaerobic biological membrane layered structure can be formed inside the carriers. The application inoculates functional sludge, domesticates the biological membrane carriers, regulates aeration intensity to build a dissolved oxygen gradient, combines with microelectrode real-time monitoring of the dissolved oxygen and oxidation-reduction potential profile inside the carriers, realizes efficient cooperation of denitrifying bacteria and methanogenic bacteria in the same biological membrane reactor, integrates anaerobic digestion and denitrification process, has the advantages of low energy consumption, small land occupation and strong impact resistance, can simultaneously realize organic matter degradation and denitrification of high-concentration organic wastewater, optimizes carbon source utilization efficiency, reduces treatment cost, is suitable for treatment of various high-concentration organic wastewater, and has important theoretical innovation value and engineering application prospect.
Owner:TIANJIN CHENGJIAN UNIV

Method for reducing soil planting obstacles and methane emission in rice-shrimp field

The present application relates to the field of ecological planting and breeding technology in rice field, in particular to a method for reducing soil planting obstacles and methane (CH4) emission in rice-shrimp field. The method provided by the present application separates and reduces the winter ponding area and time through the breeding of crayfish, increases the ventilation and oxygen content of the rice field, prolongs the decomposition time of the good nutrients of straw, and reduces the degree of gleying and improves the soil quality of the rice field; the next year, the rice is directly sowed to delay the rice flooding time, reduce the total ponding time during the rice growth period, increase the decomposition of the organic materials such as straw feed which is not used by crayfish, reduce the anaerobic decomposition of methanogens, effectively reduce the CH4 emission while ensuring the income of farmers.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

Piezoelectric biological hybrid, and preparation method and application thereof

This invention discloses a piezoelectric biohybrid, belonging to the field of wastewater treatment and resource utilization technology. The piezoelectric biohybrid is composed of barium titanate particles and anaerobic sludge, with the barium titanate particles exhibiting piezoelectricity. This invention innovatively utilizes the abundant mechanical stress generated by fluid flow, mechanical stirring, and microbial gas production in the anaerobic fermentation system. The piezoelectric biohybrid provided by this invention converts the mechanical energy of this stress into a built-in electric field within the anaerobic fermentation system, providing a new method for driving interspecies electron transfer. This effectively enhances interspecies electron transfer between acid-producing bacteria and methanogenic bacteria, increasing methane yield under anaerobic fermentation. Furthermore, the piezoelectric biohybrid provided by this invention has the advantages of simple structure, reasonable design, and ease of manufacture.
Owner:NANJING UNIV

Microbial primary battery coupling system based on anaerobic digestion and treatment method

The invention belongs to the technical field of sludge anaerobic digestion, and particularly relates to a microbial primary battery coupling system based on anaerobic digestion and a treatment method. On the basis of an anaerobic digestion and microbial primary battery coupling system, the negative influence of volatile fatty acid accumulation in an anaerobic digestion system is relieved by utilizing the catalytic action of microorganisms, the extracellular electron transfer capacity of methanogens is improved, the system stability is improved, and methane production is promoted. According to the method, the anaerobic digestion performance of the system is improved, biological electric energy can be recycled, sludge generation is reduced, and the method has the advantages of being convenient to operate and maintain, low in cost and the like and has great advantages compared with a traditional anaerobic digestion system.
Owner:SHANDONG POLYTECHNIC

Methane inhibitor based on plant functional components and use thereof

PendingCN122351272ABiotechnologyBenzoic acid
This invention discloses a methane inhibitor based on plant functional components and its application. The inhibitor, by mass fraction (100%), comprises the following raw materials: 1.5-6% p-hydroxybenzoic acid, 0.4-2.4% astragaloside, 5-40% polyethylene glycol 4000, and 81.6-99.999% bentonite. The methane inhibitor provided by this invention uses p-hydroxybenzoic acid and astragaloside as active ingredients. Through their synergistic effect, it effectively inhibits the activity of methanogenic bacteria and the methane production pathway in the rumen of ruminants, while simultaneously improving the rumen fermentation pattern. The above-mentioned methane inhibitor uses natural plant-derived active ingredients, is safe, non-toxic, environmentally friendly, and has a simple preparation process suitable for large-scale production.
Owner:INSTITUTE OF SUBTROPICAL AGRICULTURE CHINESE ACADEMY OF SCIENCES +1

Urban sewage treatment method based on EGSB (Expanded Granular Sludge Bed) and anaerobic ammonia oxidation enhanced AOOA-

The invention relates to the field of town sewage treatment, in particular to a town sewage treatment method based on EGSB (Expanded Granular Sludge Bed) and anaerobic ammonia oxidation enhanced AOOA-FBBR (Aerobic Oxidation Aerobic Comprising the following steps: S1, filling an EGSB (Expanded Granular Sludge Bed) with a polyurethane sponge filler, and respectively filling a first anoxic tank, a first aerobic tank, a second aerobic tank and a second anoxic tank of the AOOA-FBBR with quartz sand filter materials; step S2, starting stage: inoculating hydrolytic acidification bacteria, hydrogen-producing acetogenic bacteria and methanogenic bacteria in the EGSB, inoculating hydrolytic bacteria, AOB, NOB and denitrifying bacteria in the first anoxic tank, the first aerobic tank, the second aerobic tank and the second anoxic tank, and respectively inoculating anaerobic ammonium oxidation bacteria in the first anoxic tank, the first aerobic tank and the second anoxic tank; when the total nitrogen of the effluent of the second aerobic tank is reduced to 4 mg / L or below, manganese oxidizing bacteria are inoculated in the second anoxic tank, and Mn < 2 + > is added; and S3, after effluent of the second anoxic tank is stable, entering a stable stage. According to the invention, high-efficiency and low-cost removal of carbon, nitrogen and phosphorus in town sewage and carbon and phosphorus resource recovery are realized.
Owner:CHANGZHOU UNIV

A method for evaluating the influence of microplastics on methane emission in wetland soil based on soil carbon components

PendingCN122361761AMineral bindingEcological environment
This invention relates to the field of wetland ecological environment monitoring and greenhouse gas emission reduction technology, specifically to a method for assessing the impact of microplastics on methane emissions from wetland soils based on soil carbon composition. This invention assesses the degree of change in methane emissions from wetland soils after the addition of microplastics by measuring changes in the content of organic carbon components in the soil. Results show that, without relying on the detection of functional genes such as methanogens and methanogens, changes in the content of only two key carbon components—dissolved organic carbon and mineral-bound organic carbon—can accurately and quickly assess the direction and intensity of the impact of microplastics on methane emissions from wetland soils. This demonstrates the feasibility, scientific validity, and practicality of the method, providing a simple, efficient, and easily scalable new approach for assessing methane emissions from greenhouse gases under microplastic pollution stress in ecosystems.
Owner:GUANGDONG UNIV OF TECH

Method for promoting methane production by using magnetic charcoal-based microbial inoculum

The invention discloses a method for promoting methane production by using a magnetic charcoal-based microbial inoculum. The method comprises the following steps: performing magnetic modification on biochar to obtain magnetic biochar, then co-culturing the magnetic biochar and electroactive bacteria to obtain a magnetic biochar-based microbial inoculum, and finally adding the magnetic biochar-based microbial inoculum into an anaerobic digestion system containing methanogens to promote methanogenesis. The method for promoting methanogenesis by using the magnetic charcoal-based microbial inoculum has the characteristics of increasing the methanogenesis rate and increasing the abundance of methanogens, and can provide important reference for developing the technology of low-cost and efficient resource utilization of organic solid wastes.
Owner:GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY

Pasture treatments for enhanced carbon sequestration and reduction in livestock-produced greenhouse gas emissions

The subject invention provides compositions and methods for reducing deleterious atmospheric gases and / or precursors thereof using livestock feed additives and / or supplements. In preferred embodiments, a multi-purpose composition comprising one or more beneficial microorganisms and / or one or more microbial growth by-products is applied to livestock animals' feed and / or to a field or pasture where livestock animals graze. In some embodiments, the composition controls methanogenic bacteria. In some embodiments, the composition enhances carbon sequestration in the field or pasture by promoting plant health and / or growth.
Owner:LOCUS SOLUTIONS IPCO LLC

A system and method for treating organic wastewater containing high concentrations of sulfate salts

The application provides a system and method for treating high-concentration organic wastewater containing sulfate, belonging to the technical field of industrial wastewater treatment, and is used for solving the problems that in the related art, sulfate-reducing bacteria and methanogens compete for substrates, resulting in low methanogenesis efficiency, and sulfide accumulation inhibits microbial activity. The system separates and couples a methanogenesis electrochemical oxidation unit and a sulfate bioreduction and sulfur fixation unit through an ion exchange membrane and an external circuit, constructs a cross-serial multi-stage treatment structure, integrates intelligent collaborative control and sludge resourceization units, realizes the simultaneous performance of efficient methanogenesis of organic matter, deep sulfate reduction and in-situ sulfur fixation, has high removal rates of COD and sulfate in the treated effluent, has low H2S content in the produced biogas, and can recover sulfur and iron resources.
Owner:BEIJING ZHONGKE GUOYI ENVIRONMENTAL PROTECTION ENG CO LTD

System for alleviating ammonia inhibition of anaerobic digestion of medium-high temperature food waste by negative pressure coupling biogas backflow

The utility model relates to a system for relieving ammonia inhibition in anaerobic digestion of medium-high temperature kitchen waste, and relates to a system for relieving ammonia inhibition in anaerobic digestion of medium-high temperature kitchen waste. The utility model is to solve the technical problems of low methane yield, high cost and ammonia nitrogen accumulation in the anaerobic digestion process of kitchen waste. The utility model draws negative pressure at the top of the anaerobic digestion tank, has low energy consumption, and can effectively reduce the ammonia nitrogen concentration of the liquid phase part and relieve the inhibition of methanogens. In addition, a small amount of H2 exists in biogas, which can generate methane under the action of hydrogen-utilizing methanogens after being recycled back to the anaerobic digestion tank, thereby increasing the proportion of methane in biogas. The utility model reduces the ammonia nitrogen partial pressure of the gas part in the anaerobic digestion tank, relieves ammonia inhibition in the system, and simultaneously realizes resource recycling, biogas purification and efficiency improvement of biogas production, thereby providing new technical support for improving the medium-high temperature anaerobic digestion of high-protein materials represented by kitchen waste.
Owner:JIANGXI HONGCHENG ENVIRONMENT CO LTD +1

Methane production equipment

Nutrients are supplied to methanogenic bacteria supported on a carrier using a simple configuration. [Solution] The methane production apparatus 10 comprises a methane fermentation tank 12 having a methane fermentation liquid containing organic matter and methane-producing bacteria; a gas holder 14 provided above the methane fermentation tank 12, which stores the biogas produced in the methane fermentation tank 12 and has a top plate 14B and an arm 40 that moves up and down according to the amount of biogas stored; a methanation unit 20 attached to the arm 40, which moves between above and below the liquid surface S of the methane fermentation liquid in conjunction with the up and down movement of the top plate 14B and the arm 40 and has a carrier that supports methane-producing bacteria; and a hydrogen supply unit 16 that supplies hydrogen to the methane fermentation tank 12.
Owner:TOKYO GAS CO LTD +2

Anaerobic biogas preparation device based on kitchen garbage

The invention discloses an anaerobic biogas preparation device based on kitchen garbage, and relates to the technical field of kitchen garbage treatment.The anaerobic biogas preparation device comprises a squeezing box, the upper end of the squeezing box is connected with a feeding mechanism capable of conducting crushing treatment on the kitchen garbage, and a separation mechanism used for conducting solid-liquid separation on the kitchen garbage is installed in the squeezing box; a liquid discharging mechanism capable of discharging grease liquid and non-grease liquid of the kitchen garbage filtrate in a split-flow manner is arranged below the separating mechanism; a conveying auger; the discharging end of the conveying auger is communicated with the interior of the pyrolyzing furnace through a connecting pipe; through cooperation of the squeezing box, the conveying auger and the pyrolyzing furnace, the high-grease liquid is separately shunted by utilizing the liquid discharging mechanism, and only the non-grease liquid is fed into the anaerobic reactor, so that inhibition of grease on methanogens is eliminated from the source, the pyrolysis condensate and the non-grease liquid are cooperatively fermented, the utilization rate of a reaction substrate is improved, and the biogas yield is further improved.
Owner:北京朝阳环境集团有限公司

PH-responsive magnetic biochar sustained-release microspheres as well as preparation method and application thereof

The invention belongs to the technical field of biomass resource utilization and anaerobic fermentation, and relates to a pH response type magnetic biochar sustained-release microsphere as well as a preparation method and application thereof. The sustained-release microsphere has a core-shell structure and comprises a functional core and a pH response shell wrapping the functional core, the functional core comprises magnetic charcoal, an alkaline buffer agent and a methanogen accelerant; the pH response shell is polyelectrolyte composite hydrogel formed by ionic crosslinking of sodium alginate and chitosan. Through the design of the core-shell structure, active sensing and intelligent regulation and control of pH abnormity of the anaerobic fermentation system are realized, and rancidity of the system is effectively prevented; and meanwhile, by utilizing multiple synergistic effects of the functional kernel, the operation stability and the methane conversion efficiency of the system are remarkably improved.
Owner:NORTHWEST ENGINEERING CORPORATION LIMITED

A process for stabilizing an anaerobic fermentation system

The present application relates to the environmental protection treatment of poultry manure, in particular to a process for stabilizing an anaerobic fermentation system, the present application collects the manure with a total concentration of 6% concentration, carries out anaerobic fermentation, improves the types of antibiotics, promotes the activity of hydrogenophilic methanogen flora, and improves the electrode material of microbial electrolysis cell, so that the obtained anaerobic fermentation system is not only high in stability, but also rich in methane production.
Owner:JIANGXI ZHENGHE ECOLOGICAL AGRI CO LTD

Preparation method of efficient biological fertilizer based on quantum tunneling effect regulation and control

The invention discloses an efficient biological fertilizer preparation method based on quantum tunneling effect regulation and control, relates to the technical field of agricultural biology, in particular to an efficient biological fertilizer preparation method based on quantum tunneling effect regulation and control, and the method comprises the following steps: screening activated syntrophic bacteria and methanogens, and constructing a fermentation system in a customized anaerobic reactor. A nanometer microenvironment monitoring probe, a pressure regulation and control module and a temperature control device which are arranged in the reactor are used for monitoring and regulating and controlling the fermentation environment in real time, and the quantum tunneling efficiency of microbial electron transfer is accurately maintained in the optimal interval of 0.7-0.9. Feeding and output of materials are completed through a strain inoculation opening, a substrate feeding opening and a product discharging opening. The bio-fertilizer prepared by the method can significantly increase the crop yield, effectively reduce the bio-availability of soil heavy metals and degrade pesticide residues, and has the functions of efficient nutrient supply and soil environment remediation.
Owner:伍修建

Device for producing methane from high-concentration manure and straw under partitioned temperature control

The utility model discloses a device for producing methane from high-concentration manure straws under zoned temperature control. Comprising a fermentation tank body, a first temperature maintaining unit and a second temperature maintaining unit which are respectively coated on the outer wall of the fermentation tank body, and a temperature buffering unit which is coated on the outer wall of the fermentation tank body and is positioned between the first temperature maintaining unit and the second temperature maintaining unit, the second temperature maintaining unit corresponds to a methane reaction area at the lower part of the fermentation tank body; and the temperature buffering unit is positioned at the junction of the acidification reaction area and the methane reaction area. The device realizes zoned temperature control of the fermentation tank body, is beneficial to providing respective appropriate temperatures for different strains in each reaction stage, improves the hydrolytic acidification efficiency of fermentation materials, improves the efficiency of methanogens and the yield of methane, can avoid excessive acidification of a fermentation system, and reduces the production cost. The feeding and discharging balance of the anaerobic fermentation system is maintained, and the efficiency of the whole material fermentation process is improved.
Owner:LESHAN NORMAL UNIV

A method for producing medium chain fatty acids from straw based on des additive

ActiveCN115029393BFermentationBiological synthesisMethanobacterElectron donor
This invention provides a method for producing medium-chain fatty acids (MCFAs) from straw based on DES additives, belonging to the field of MFA extraction technology. This method uses DES with choline chloride as the hydrogen bond acceptor to treat agricultural waste straw, improving the utilization rate of agricultural waste and promoting the anaerobic fermentation process for MFA production. Pretreatment before anaerobic fermentation and the thermal shock effect during anaerobic fermentation improve the depolymerization of straw, inhibit methanogenic bacteria in mesophilic anaerobic fermentation, suppress methane production during continuous anaerobic fermentation, and ensure maximum utilization of straw for MFA production. This method solves the problems of methanogenesis tendency, incomplete substrate utilization, insufficient electron donors during acid production, and low fermentation efficiency in the anaerobic fermentation of straw for MFA production.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

Straw fermentation methanogenic saccharomyces cerevisiae engineering bacteria and preparation method and application thereof

PendingCN122326418ABiotechnologyMethanobacter
The application provides a kind of straw fermentation methane Saccharomyces cerevisiae engineering bacteria and its preparation method and application, belong to microbial engineering technical field.The Saccharomyces cerevisiae engineering bacteria is constructed by knocking out one or more of ALD6 gene, ADH1 gene and ADH4 gene of Saccharomyces cerevisiae BY4741.The Saccharomyces cerevisiae engineering bacteria can effectively regulate the dynamic balance of acid production and consumption in fermentation system, alleviate the inhibition of acidification in the early stage of fermentation, improve the stability of microenvironment system, provide suitable conditions for the growth and metabolism of methanogens, optimize the coupling matching degree of substrate hydrolysis efficiency and methanogenesis efficiency, and significantly improve the cumulative methanogenesis and gas production stability of straw anaerobic fermentation.The preparation method is simple and easy to scale up.The method provided by the application is convenient for straw fermentation methane, does not need to adjust the pH of fermentation system, adapts to industrial straw anaerobic fermentation process, and can realize efficient resource utilization of agricultural wastes such as wheat straw.
Owner:SHENZHEN RESEARCH INSTITUTE OF NORTHWEST A & F UNIVERSITY +1

A co-processing device for food waste and sludge

This utility model discloses a co-treatment device for kitchen waste and sludge, including a raw water tank for mixing the kitchen waste and sludge to form a mixed liquid, an anaerobic tank, a UASB tank, a multi-functional tank, and an internal circulation pipeline. The device independently controls carbon production and methanogenesis, with corresponding anaerobic tanks and UASB tanks. Through this modular and partitioned design, technicians can adjust the hydraulic retention time and pH according to the target to achieve dynamic metabolic balance, avoiding the limitations of a single-target process, and realizing the autonomous adjustment of the metabolic balance between acid-producing bacteria and methanogenic bacteria.
Owner:KUNSHAN SEWAGE TREATMENT CO