92 results about "Methanobacter" patented technology

To provide a methanation system which has good efficiency of conversion of carbon dioxide to methane even when gas containing a low concentration of carbon dioxide is used as a carbon dioxide source, and which enables simplification of equipment and reduction of installation area thereof.SOLUTION: Methanation equipment is provided, comprising: a dissolution tank exposing carbon dioxide-containing gas to an aqueous phase, thereby producing carbon dioxide-containing solution; a culture tank for culturing methane in culture solution, thereby producing methane; a carbon dioxide gas supply flow passage for introducing carbon dioxide-containing gas into the dissolution tank; solution transfer means transferring the carbon dioxide-containing solution produced in the dissolution tank from the dissolution tank to the culture tank; a hydrogen supply flow passage for supplying hydrogen to the dissolution tank or the culture tank; and a methane discharge flow passage for discharging the produced methane from the culture tank.SELECTED DRAWING: Figure 1

The invention provides an intelligent optimization method for carbon emission reduction of an ecological pasture in extreme weather, and relates to the technical field of intelligent optimization of carbon emission reduction. Comprising the steps that initial monitoring data are collected and preprocessed, and multi-dimensional feature vector output state features are constructed; an LSTM time sequence prediction model is used for analyzing the state of the dairy cow and the activity of methanogens in the rumen, DHI data of an integrated gradient boosting tree model is used for analyzing the amino acid balance and precise nutrition technology of the lactating dairy cow, a dairy cow manure yield prediction model based on a Bayesian network is constructed, a manure management strategy is dynamically optimized, and manure management effect data is output. And constructing a carbon emission reduction comprehensive evaluation model, and evaluating the pasture carbon emission reduction effect. According to the invention, the rumen flora structure is regulated and controlled, the rumen greenhouse gas emission is controlled, the ruminant feed protein level is reduced, and the methane emission is reduced by researching the dairy cow carbon emission rumen microorganism regulation and control technology.

The invention discloses a preparation method of salt-tolerant methanogen liquid for treating high-salt steroid pharmaceutical wastewater, and belongs to the technical field of anaerobic digestion treatment of high-salt pharmaceutical wastewater. According to the technology, the salt-tolerant methanogenic flora is domesticated and enriched through acetic acid and high salt dual stress, and then the salt-tolerant methanogenic bacterial liquid is prepared through passage enrichment, so that a salt-tolerant methanogenic strain is provided for biologically enhancing the anaerobic treatment performance of the high-salt steroid pharmaceutical wastewater, the COD (Chemical Oxygen Demand) removal efficiency and methanogenic efficiency of the high-salt steroid pharmaceutical wastewater are effectively improved, and the method is suitable for industrial production. The conversion of VFAs is accelerated, so that a rapid, low-cost and high-efficiency treatment method can be provided for efficient anaerobic treatment of high-salt steroid pharmaceutical wastewater.

The utility model belongs to sewage treatment technical field, specifically is a kind of sludge reduction's sewage treatment equipment, including base, the base top is fixed with processing cylinder, the processing cylinder bottom is fixed with bottom plate, by setting movable cylinder, second movable shaft, stirring plate, stirring rod and stirring ring, can make sludge particle in sewage even suspended, let hydrolytic bacteria, methanogen etc. and the organic matter in sludge fully contact, accelerate hydrolysis, acidification and the rate of methanogenesis reaction, reduce substrate residue, and anaerobic sludge is kept for a long time, can form dense precipitate layer in processing cylinder bottom, lead to effective reaction volume shrinkage, stirring can make sludge keep suspended state, maintain the effective volume utilization rate of processing cylinder, while anaerobic treatment needs stable temperature, if not stirring, local temperature difference in processing cylinder is easy to appear, lead to microbial activity imbalance, by stirring can maintain the uniformity of sewage anaerobic environment, stable reaction condition.

The present application relates to the technical field of excrement treatment, and particularly relates to a feces treatment method based on flora analysis, comprising obtaining reaction temperature and material Ph value and determining gas production relative deviation based on gas production, determining device operation state based on characteristic parameters; in response to the device operation state, determining abnormal characteristic identification to obtain key functional flora parameters, determining flora balance index based on the key functional flora parameters to determine whether acid-producing bacteria and methanogenic bacteria are in flora balance; determining smoothness index according to the change curve of a plurality of actual flora balance indexes obtained in an initial detection period to determine the deviation degree of the flora balance index, and adjusting the correction sensitivity coefficient of the feed adjustment amount based on the smoothness index. The present application performs real-time monitoring and dynamic regulation on the excrement treatment process based on the flora analysis result to maintain optimal flora structure and metabolic activity, and improves treatment efficiency and stability.

The invention provides a material and a method for cleaning biogas residues produced by anaerobic digestion of waste oil reservoirs. The method comprises the following steps: (1) determining required anaerobic digestion methanogens and substrates according to the types of biogas residues produced by anaerobic digestion of the waste oil reservoir; (2) the oil reservoir is screened according to the requirements of the anaerobic digestion methanogens and the substrate for temperature, mineral substances, pH value and permeability, and a standard rock core is obtained; (3) adopting a physical simulation method, injecting anaerobic digestion methanogens and different organic matters into the standard rock core to clean in a manner of producing biogas residues through anaerobic digestion, and performing biogas residue cleaning test on the biogas residues generated by the different organic matters to obtain a cleaning scheme; and (4) cleaning the biogas residues produced by anaerobic digestion of the waste oil reservoir according to the cleaning scheme. A classification cleaning strategy is designed according to the blocking risk of organic matter biogas residues of different sources, the method for preparing the biogas through anaerobic digestion of the waste oil reservoir can be achieved, and the in-situ biogas preparation efficiency is improved.

The invention discloses a binary pseudocapacitance composite material for promoting anaerobic digestion of kitchen waste and a preparation method and application thereof, and belongs to the technical field of organic solid waste recycling and renewable energy sources. The wood chip biochar-polydopamine-Fe3O4 composite material with a core-shell structure is constructed by taking wood chips as raw materials, preparing biochar through low-temperature pyrolysis, forming an interface modification layer through polydopamine coating, loading Fe < 3 + > and carrying out secondary pyrolysis to generate Fe3O4 nanoparticles (40-110nm). The material can be recovered through a magnetic field (the recovery rate is greater than or equal to 90%), when the dosage is 0.8-1.2 g / L, the peak value of volatile fatty acid in an anaerobic digestion system is obviously reduced to be less than or equal to 400 mg / L, the methane accumulation yield is improved to be greater than or equal to 45%, the electron transfer efficiency of acid-producing bacteria and methanogens is synchronously optimized, and the system operation is stabilized.

The application provides a method for enhancing anaerobic digestion of organic solid waste containing new pollutant antibiotics by circulating oxygen disturbance, and belongs to the technical field of solid waste resource treatment. The method introduces circulating and increasing micro-nano bubble oxygen water stress during the stable operation period of anaerobic digestion, uses the dynamic change of physical oxidation pressure to induce the adversity defense mechanism of microorganisms, and promotes the improvement of metabolic activity and optimization of community structure of functional flora in situ. By accurately regulating the key process parameters such as oxygen disturbance cycle, gradient dosing ratio and the like, the application not only avoids the excessive inhibition of oxygen on methanogens, but also significantly enhances the adaptability of the system to the environmental stress of new pollutants. The application has the advantages of no need for external agents, high physical mass transfer efficiency, significant yield increase and harmless effect, and is suitable for in-situ strengthening and deep performance improvement of anaerobic digestion systems of municipal sludge, livestock and poultry manure and kitchen garbage and the like.

The application discloses an aerobic-anaerobic bacteria combined coal pretreatment biological gas production device, which comprises a gas blowing system, a bacterial population pre-culturing system, an aerobic-anaerobic biological combined pretreatment and gas production system and a gas-liquid two-phase collection and monitoring system. The application also discloses a gas production method of the gas production device. The application takes coal as a substrate, and under suitable conditions, carries out culture and optimization of a methanogenic bacterial population, and simulates indoor biological gas production effects under different coal rank, bacterial type, combined pretreatment mode, treatment time and metabolic environment conditions. The application adopts an integrated combined processor to combine aerobic bacteria and anaerobic bacteria for pretreating coal, so that coal-based biological gas production can be safely and efficiently carried out, and the application has important environmental benefits and broad application prospect.

The invention discloses a method for rapidly constructing denitrification type anaerobic methane oxidation through hydrogen type methanogens, and relates to the technical field of sewage treatment. According to the method, anaerobic digestion sludge of a sewage treatment plant is taken for inoculation, and a methanogen-mediated denitrification type anaerobic methane oxidation process is constructed. Hydrogen-type methanogens in the anaerobic digestion system excite a reverse methanogenesis function under the condition that organic matters are deficient, and denitrification anaerobic methane oxidation is realized by coupling sulfate and nitrate with nitrogen and sulfur circulating microorganisms. Sulfate is used as a potential electron acceptor in sewage, and dissoluble methane is removed while sulfate is indirectly utilized through recessive biological sulfur cycle catalytic bridging anaerobic methane oxidation and denitrification. In addition, the anaerobic digestion sludge can be continuously inoculated with hydrogen-type methanogens, and compared with traditional denitrification type anaerobic methane oxidation archaea culture, the method is more convenient. Therefore, the denitrifying anaerobic methane oxidation rapidly constructed by the hydrogen-type methanogens is an effective measure for breaking through the deep denitrification of the sewage treatment plant and synchronously controlling and discharging the greenhouse gas.

The present invention provides a method for producing methane by dry anaerobic fermentation, which relates to the technical field of anaerobic fermentation. In the present invention, a biochar-immobilized complex enzyme is added during the dry anaerobic fermentation process. The biochar-immobilized complex enzyme is achieved by fixing the enzyme to biochar, which can enhance the stability and resistance to protein degradation of the enzyme, extend the service life of the enzyme, and reduce the inactivation rate of the enzyme, thereby facilitating an increase in methane production. In addition, biochar has a high specific surface area and the characteristics of promoting interspecies electron transfer, which is conducive to increasing methane production. Moreover, in the biochar-immobilized complex enzyme, biochar fixes the enzyme on its surface or interior, which can provide more reactive sites, which is conducive to the enrichment of methanogens, thereby shortening the fermentation reaction cycle and facilitating an increase in the reaction rate of anaerobic fermentation. In summary, the reaction rate and methane yield of the method for producing methane by dry anaerobic fermentation provided by the present invention are both high.

The invention provides a method for preparing biogas by anaerobic digestion of a waste oil reservoir, which comprises the following steps of: determining required anaerobic digestion methanogens and substrates according to geological characteristics, temperature, salinity and residual oil components of a target waste oil reservoir; the abandoned oil reservoirs are screened through the geological parameters and the well pattern mode, and target oil reservoirs are obtained; arranging a well pattern form in which vertical wells and horizontal wells are combined on a target oil reservoir; generating an injection scheme by adopting a physical simulation method; wherein the injection scheme comprises the injection amount and the injection concentration of anaerobic digestion methanogens and substrates of a target oil reservoir; according to the injection scheme, the anaerobic digestion methanogens and the substrate are injected into the target oil reservoir in combination with the well pattern, anaerobic digestion is carried out in the oxygen-free environment, and the biogas is obtained. The natural temperature, mineralization degree, anaerobic environment and other environments of the waste oil reservoir are utilized, substrates and florae are fully stirred and injected into the waste oil reservoir, and then anaerobic digestion is carried out underground through bacteria in an anaerobic environment to generate methane gas.

A microbial culture tank (10) comprises: a riser tank (11) having a structure for raising a culture liquid (S) that cultures methane-producing bacteria that generate methane (M); a downcomer tank (12) having a structure for lowering the culture liquid (S) overflowing from the riser tank (11); and a recovery section (13) for recovering methane (M) released from the culture liquid (S) in the riser tank (11) and the downcomer tank (12).

The invention discloses an MEC reactor for promoting sludge deamination and strengthening anaerobic digestion capability by coupling oxides, and relates to the field of anaerobic digestion treatment of sludge in a sewage treatment plant and resource utilization of biogas, the MEC reactor comprises an MEC reactor, an oxidation-reduction potential detection device, a hydrogen detection device, a PLC control module and an oxide adding device; the oxide adding device takes air as an oxide, the air is converted into micro-nano bubbles through a microporous aerator, and ammonia nitrogen is oxidized into nitrate nitrogen; oRP and hydrogen partial pressure in the MEC reactor are monitored in real time through the oxidation-reduction potential detection device and the hydrogen detection device respectively and transmitted to the PLC control module, and the ORP and the hydrogen partial pressure in the MEC reactor main body are intelligently and accurately controlled according to a negative feedback regulation mechanism. The MEC reactor disclosed by the invention relieves the inhibition effect of partial pressure of ammonia nitrogen and hydrogen on methanogens while deaminizing the sludge, improves the biogas yield and strengthens the anaerobic digestion capability of the MEC reactor.

The application discloses a kind of microbial oil extraction methods using green plants as raw material, comprising the following steps: (1) target reservoir screening;(2) target reservoir endogenous microbial community structure analysis;(3) target reservoir methanogen analysis;(4) green plant injection preparation;(5) green plant injection on-site injection, the application is applicable to the field of microbial oil extraction technology, for the reservoir that complete flora exists in endogenous microorganism, on the one hand, carbon fixation is used to green plants, and the emission of carbon dioxide is reduced;On the other hand, endogenous microorganism in reservoir uses green plant injection as nutrition, and acid and gas are produced after degradation, reduce the cost of microbial oil extraction technology, greatly improve the effect of microbial oil extraction;For the reservoir without complete flora, by supplementing functional bacteria, various complex organic matter can be hydrolyzed into relatively simple organic matter, and anaerobic fermentation is carried out to produce gas, effectively improve the recovery efficiency of crude oil, improve the development effect of middle and late reservoir.

The invention discloses a tooth-benefiting xylitol tablet candy and a preparation method thereof. The tooth-benefiting xylitol tablet candy is prepared from the following raw materials: xylitol, modified sodium alginate, HPMCP-genipin, microcrystalline cellulose, colloidal silicon dioxide and magnesium stearate. After the HPMCP-genipin enters the enteric cavity, the shell is dissolved, the L-glutamine and the anhydrous dextrose maintain and stabilize the osmotic pressure of intestinal cells, the genipin of the inner core and the primary amine group on the molecular chain of the modified sodium alginate are covalently crosslinked to form a gel network to wrap the xylitol, and meanwhile, the gel network absorbs water to form a local isotonic environment. A gel network wrapping xylitol enters a large intestine and is dissolved under the action of coliform bacteria, and galactooligosaccharide selectively stimulates the growth of bacteria methanogens capable of utilizing hydrogen, so that the total volume and partial pressure of gas in the intestinal tract are reduced. The microcrystalline cellulose improves the viscous component of the modified sodium alginate, and the colloidal silicon dioxide reduces the shearing and extrusion on the HPMCP-genipin in the tabletting process.

The invention discloses a bypass type two-phase anaerobic process and system for kitchen waste treatment and a kitchen waste treatment plant comprising the system, and relates to the technical field of kitchen waste treatment.The bypass type two-phase anaerobic process comprises the following steps that organic slurry obtained through kitchen waste pretreatment is fed into an adjusting tank to be stored; the organic slurry is fed into a hydrolytic acidification tank, macromolecular organic matters in the organic slurry are converted into micromolecular matters by hydrolytic bacteria in the hydrolytic acidification tank, and the micromolecular matters are converted into organic acid, hydrogen and carbon dioxide by acidizing bacteria; the organic slurry subjected to hydrolytic acidification is fed into an anaerobic reaction tank, and organic acid in the organic slurry is converted into methane through methanogens in the anaerobic reaction tank; when the pH in the anaerobic reaction tank is 1t; 6.5 or VFAgt, or VFAgt; when the concentration is 3000 mg / L, the organic slurry subjected to hydrolytic acidification flows back to the regulating tank. According to the invention, the amount of slurry entering the anaerobic reaction tank can be flexibly controlled, the feeding load is balanced, and the load impact of the anaerobic reaction tank is effectively buffered, so that the retention time of the anaerobic reactor can be prolonged.

The application discloses an anaerobic treatment system and process suitable for wastewater containing high organic suspended matter, which comprises a water inlet assembly, a reactor, an internal circulation assembly, a water collecting and discharging assembly and an exhaust assembly; a group of metal membrane tubes are arranged in the reactor, and hydrolytic acid-producing bacteria are loaded in the metal membrane tubes; methanogenic bacteria are filled in the space outside the metal membrane tubes in the reactor; the reactor is connected with the water inlet assembly through the bottom of the metal membrane tubes; the internal circulation assembly is connected with the upper and lower parts of the reactor respectively; the water collecting and discharging assembly is arranged on the top of the reactor, and the reaction water is discharged from the reactor through a discharging pipeline; and the exhaust assembly is connected with the top of the reactor. The application can realize the effect that the acid-producing phase and the methanogenic phase are separated from each other and cooperate with each other, the effluent water is filtered through a membrane, and stable three-phase separation is realized. The application has the advantages of high anaerobic reaction efficiency, stable effluent water quality and high methane yield, and is especially suitable for anaerobic treatment of wastewater containing high organic suspended matter.

The present invention relates to a high-value CO2 gas conversion device based on micro-high-voltage electrochemical technology. The device includes a heating device, a sealed housing, a working electrode, a counter electrode, a reference electrode, and a stirring device. The heating device is arranged to surround the sealed housing. The sealed housing is provided with a cathode chamber and an anode chamber. The cathode chamber contains homoacetogenic bacteria or methanogenic bacteria. The working electrode and the reference electrode extend from the outside of the top cover of the sealed housing into the cathode chamber. The counter electrode extends from the outside of the top cover of the sealed housing into the anode chamber. The stirring device extends from the outside of the top cover of the sealed housing into the cathode chamber and the anode chamber, respectively. The top cover of the sealed housing is provided with liquid inlets and gas inlets and outlets, which are connected to the cathode chamber and the anode chamber. The device uses water and CO2 / CO as raw materials to produce high-value chemicals through microbial electrosynthesis.

The present invention provides a method for hydrolyzing and acidifying agricultural waste and a method for synthesizing medium-chain fatty acids, belonging to the technical field of resource utilization of agricultural waste. The present invention provides a method for hydrolyzing and acidifying agricultural waste, comprising: adjusting the solid content of the agricultural waste, mixing it with short-chain AHLs and chitosan microspheres loaded with DPD to obtain a hydrolysis and acidification system; and subjecting the hydrolysis and acidification system to anaerobic fermentation to obtain a hydrolysis and acidification product. This method adds short-chain AHLs during the hydrolysis and acidification process of agricultural waste to activate genes related to hydrolysis and acidification bacteria, promote biofilm formation and extracellular enzyme secretion, improve substrate hydrolysis and acidification efficiency, and promote substrate decomposition and short-chain fatty acid accumulation; DPD is delayed in release during the metabolic peak of acid-producing bacteria, coordinating the metabolic division of hydrolytic bacteria and acid-producing bacteria, inhibiting the activity of methanogens, reducing substrate competition, improving the degradation rate of agricultural waste cellulose, and increasing the yield of medium-chain fatty acid precursors such as acetic acid in the fermentation substrate.

The present application relates to wastewater resource treatment technical field, especially to a kind of magnetic field reinforced anaerobic reactor and wastewater resource treatment method.The magnetic field reinforced anaerobic reactor provided by the present application, including anaerobic reactor body and multiple magnetic blocks fixed on the outer surface of the anaerobic reactor body, wherein the multiple magnetic blocks are divided into multiple rows and circled on the outer surface of the anaerobic reactor body, and the magnetic blocks in each row are arranged in the order of left, upper, right and down according to the direction of magnetic field.The magnetic field reinforced anaerobic reactor of the present application can convert the movement of microorganisms in the reactor relying on water flow, bubble or itself rising or falling into cutting magnetic induction line movement by using magnetic field, convert kinetic energy into potential energy, enhance the electron transfer between acetogenic bacteria and methanogens, improve the utilization of organic matter in wastewater by acid-producing bacteria and the process of methanogens utilizing electron to reduce carbon dioxide to produce methane, and realize the improvement of energy utilization and methanogenesis efficiency in anaerobic reactor.

The application provides a device and method for regulating biological membrane niche to realize simultaneous methanogenesis and denitrification, and belongs to the technical field of water treatment, comprising a biological membrane reactor, a water distribution tank, a peristaltic pump, an aeration system and an electric stirrer, the biological membrane reactor is filled with biological membrane carriers, and an anoxic-anaerobic biological membrane layered structure can be formed inside the carriers. The application inoculates functional sludge, domesticates the biological membrane carriers, regulates aeration intensity to build a dissolved oxygen gradient, combines with microelectrode real-time monitoring of the dissolved oxygen and oxidation-reduction potential profile inside the carriers, realizes efficient cooperation of denitrifying bacteria and methanogenic bacteria in the same biological membrane reactor, integrates anaerobic digestion and denitrification process, has the advantages of low energy consumption, small land occupation and strong impact resistance, can simultaneously realize organic matter degradation and denitrification of high-concentration organic wastewater, optimizes carbon source utilization efficiency, reduces treatment cost, is suitable for treatment of various high-concentration organic wastewater, and has important theoretical innovation value and engineering application prospect.

The invention belongs to the technical field of biogas preparation, and particularly relates to a method for preparing biogas through intermittent aeration coupling ethanol type fermentation and electric field polarization intensification. According to the method, a non-biological methanogenesis composite reflux way is adopted, an alcohol fermentation base material is refluxed to a hydrolysis area, alcohol reacts with free radicals generated by intermittent aeration to generate a methane precursor, and methane generation is promoted; meanwhile, alcohol fermentation is an effective means for constructing novel direct interspecific electron transfer, the thermodynamic bottleneck of traditional acetic acid production and hydrogen nutrition type methane production can be avoided, the mass transfer efficiency and the system stability are improved, and the anaerobic reactor can effectively produce gas at the pH value of 3.5-8; free radicals generated by intermittent aeration and oxidation-reduction oscillation can promote decomposition of a substrate in a hydrolysis area through free radical generation and facultative bacterium growth; the activity of respiratory enzyme is enhanced through the coupling electric field and electric field polarization, the yield of free radicals and alcohol and the metabolism of methanogens are improved, and the yield of biogas is systematically improved.

The application discloses a system and method for improving acid production efficiency of organic wastewater hydrolysis acidification, comprising a methanogen inhibition tank, a seeding sludge feeding pump, a hydrolysis acidification tank, an alkali adding device, an organic wastewater inlet pipeline and a temperature controller; an inhibition tank outlet on the bottom side of the methanogen inhibition tank is connected with a seeding sludge inlet on the top side of the hydrolysis acidification tank through the seeding sludge feeding pump; a dosing opening and an exhaust opening are arranged on the top of the hydrolysis acidification tank; the alkali adding device is connected with the dosing opening; the organic wastewater inlet pipeline is connected with a water inlet on the bottom of the hydrolysis acidification tank through the temperature controller; the system and method have higher acid production efficiency, and are not prone to produce poor carbon sources.

The invention discloses a cyanobacteria bottom mud fermentation methanogenic bacterial agent, which comprises clostridium butyricum, bacillus amyloliquefaciens and methanosarcina martensii, and is characterized in that the clostridium butyricum accounts for 30-60% of the total number of bacterial cells, the bacillus amyloliquefaciens accounts for 25-50% of the total number of the bacterial cells, and the methanosarcina martensii accounts for 30-60% of the total number of the bacterial cells. The methanosarcina marzeberi accounts for 10%-25% of the total number of bacterial cells. The advanced technology of adding microbial compound bacteria and combining high-temperature and high-pressure hydrolysis and anaerobic digestion technologies is utilized, the biodegradability is improved by destroying the cell structure of organic matter, the efficiency and stability of traditional anaerobic fermentation are remarkably improved, the VS removal rate after pyrohydrolysis fermentation reaches 67.5%-70.4%, the gas production rate in the anaerobic fermentation process after pyrohydrolysis is effectively increased, and the production cost is reduced. The yield of methane can be increased, the water body environment can be improved, recycling of bottom mud resources can be promoted, and green and sustainable ecological restoration is achieved.

Provided is a methanation system that has good efficiency of conversion of carbon dioxide to methane even when a gas containing a low concentration of carbon dioxide is used as a carbon dioxide source, and enables simplification and footprint reduction of an apparatus. This methane production apparatus comprises: a dissolution tank for exposing carbon dioxide gas to an aqueous phase to produce a carbon dioxide solution; a culture tank for culturing methane-producing bacteria to produce methane; a carbon dioxide gas supply flow passage for feeding carbon dioxide gas into the dissolution tank; a solution transfer means for transferring the carbon dioxide solution from the dissolution tank to the culture tank; a hydrogen supply flow passage for supplying hydrogen; and a methane discharge flow passage for discharging the produced methane from the culture tank.

The present application relates to the field of ecological planting and breeding technology in rice field, in particular to a method for reducing soil planting obstacles and methane (CH4) emission in rice-shrimp field. The method provided by the present application separates and reduces the winter ponding area and time through the breeding of crayfish, increases the ventilation and oxygen content of the rice field, prolongs the decomposition time of the good nutrients of straw, and reduces the degree of gleying and improves the soil quality of the rice field; the next year, the rice is directly sowed to delay the rice flooding time, reduce the total ponding time during the rice growth period, increase the decomposition of the organic materials such as straw feed which is not used by crayfish, reduce the anaerobic decomposition of methanogens, effectively reduce the CH4 emission while ensuring the income of farmers.

This invention discloses a piezoelectric biohybrid, belonging to the field of wastewater treatment and resource utilization technology. The piezoelectric biohybrid is composed of barium titanate particles and anaerobic sludge, with the barium titanate particles exhibiting piezoelectricity. This invention innovatively utilizes the abundant mechanical stress generated by fluid flow, mechanical stirring, and microbial gas production in the anaerobic fermentation system. The piezoelectric biohybrid provided by this invention converts the mechanical energy of this stress into a built-in electric field within the anaerobic fermentation system, providing a new method for driving interspecies electron transfer. This effectively enhances interspecies electron transfer between acid-producing bacteria and methanogenic bacteria, increasing methane yield under anaerobic fermentation. Furthermore, the piezoelectric biohybrid provided by this invention has the advantages of simple structure, reasonable design, and ease of manufacture.

The invention belongs to the technical field of sludge anaerobic digestion, and particularly relates to a microbial primary battery coupling system based on anaerobic digestion and a treatment method. On the basis of an anaerobic digestion and microbial primary battery coupling system, the negative influence of volatile fatty acid accumulation in an anaerobic digestion system is relieved by utilizing the catalytic action of microorganisms, the extracellular electron transfer capacity of methanogens is improved, the system stability is improved, and methane production is promoted. According to the method, the anaerobic digestion performance of the system is improved, biological electric energy can be recycled, sludge generation is reduced, and the method has the advantages of being convenient to operate and maintain, low in cost and the like and has great advantages compared with a traditional anaerobic digestion system.

This invention discloses a methane inhibitor based on plant functional components and its application. The inhibitor, by mass fraction (100%), comprises the following raw materials: 1.5-6% p-hydroxybenzoic acid, 0.4-2.4% astragaloside, 5-40% polyethylene glycol 4000, and 81.6-99.999% bentonite. The methane inhibitor provided by this invention uses p-hydroxybenzoic acid and astragaloside as active ingredients. Through their synergistic effect, it effectively inhibits the activity of methanogenic bacteria and the methane production pathway in the rumen of ruminants, while simultaneously improving the rumen fermentation pattern. The above-mentioned methane inhibitor uses natural plant-derived active ingredients, is safe, non-toxic, environmentally friendly, and has a simple preparation process suitable for large-scale production.