Domestication method of ammonia nitrogen and propionic acid resisting methane production strain

A technology that is resistant to ammonia nitrogen and methanogenic bacteria, applied in the field of microorganisms, can solve the problems of harsh microbial culture conditions, difficulty in resisting indigenous microorganisms, high ammonia nitrogen concentration, etc., to achieve easy transportation and long-term storage, improve biogas fermentation performance, and shock resistance strong effect

Active Publication Date: 2017-05-31
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Application Information

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Problems solved by technology

There are following problems in the preparation technology of these bacterial agents: the first, the anaerobic microbial culture conditions are harsh, need special anaerobic equipment and microbiological professional technical personnel to cultivate; It is difficult for strains added from external sources to adapt to the complex environmental conditions in the anaerobic fermentation system, such as low pH, high ammonia nitrogen conc

Method used

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  • Domestication method of ammonia nitrogen and propionic acid resisting methane production strain

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Embodiment 1

[0021] The biogas fermentation liquid of the inoculum in this example is a kitchen waste fermentation liquid, the organic load is 1.0 g / L d, and the daily volumetric gas production rate is 0.3 L / L d. (also can choose the fermented liquid of other arbitrarily stable operation fermenter).

[0022] The culture device is a 1L reagent bottle, which is equipped with a rubber stopper with two holes, one of which is inserted with a rubber tube to the middle and lower part of the reagent bottle as a passage for feeding and discharging materials, and the other hole is connected to an air bag for collecting gas .

[0023] The acclimatization method of the ammonia-zirpropionic acid-resistant methanogenic strain of the present embodiment comprises the following steps: in a mixed atmosphere with a volume ratio of nitrogen and carbon dioxide of 4:1, to ensure an anaerobic environment, inoculate 950 ml of the inoculum until the concentration of propionic acid is 0.5g / L ammonia nitrogen conce...

Embodiment 2

[0028] Embodiment 2: the anaerobic fermentation bioaugmentation effect of the ammonia-zirpropion-resistant methanogenic bacteria agent obtained by the present invention on kitchen waste

[0029] Fermentation raw materials: artificially prepared food waste, which is composed of rice flour, milk powder and whole egg powder. The ratio of the three ingredients is rice flour: protein powder: milk powder is 1:1:1, and the C / N is 20.

[0030] Inoculum: anaerobic fermentation broth of food waste.

[0031] Reaction device: 2L continuous CSTR online automatic gas recovery device.

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Abstract

The invention discloses a domestication method of an ammonia nitrogen and propionic acid resisting methane production strain. The ammonia nitrogen and propionic acid resisting methane production strain comprises a propionic acid oxidization flora, a syntrophaceae propionic acid oxidization flora, an acetic acid cracking methane production flora, a hydrogen nutrition type methane production flora and a denitrification flora. The method takes marsh gas fermentation liquid as an inoculum, takes propionic acid as a carbon source and takes urea or NH4Cl as an ammonia nitrogen source; the marsh gas fermentation liquid is domesticated through a method for alternately improving the concentration of the propionic acid and the concentration of ammonia nitrogen, so as to obtain the ammonia nitrogen and propionic acid resisting methane production strain; the strain can stably produce gas for a long period under the conditions that the concentration of the ammonia nitrogen is more than 3.0g/L and the concentration of the propionic acid is more than 2.0g/L; the gas yield is 60 percent more than a theoretical yield; the utilization rate of the propionic acid and acetic acid is more than 90 percent; the ammonia nitrogen and propionic acid resisting methane production strain has the advantages of high impact resistance, high stability of biological enhancing effect and the like, and can be used for removing comprehensive inhibition of the ammonia nitrogen and the propionic acid in a marsh gas fermentation process and improving the fermentation performance of marsh gas.

Description

[0001] Technical field: [0002] The invention relates to the technical field of microbes, in particular to a domestication method of an ammonia-ziridine-resistant methanogenic strain. [0003] Background technique: [0004] Under the dual pressure of energy shortage and environmental pollution, the biogas industry, which can not only process waste but also generate renewable energy, has developed rapidly, from small-scale household biogas to large-scale production. According to statistics, there are about 43 million biogas users in the country, and there are about 100,000 large-scale biogas projects nationwide. However, most biogas digesters are idle because they cannot operate stably for a long time, and most biogas projects are still unprofitable. The acid inhibition problem caused by the accumulation of volatile organic acids is one of the factors restricting the stability and economy of biogas fermentation. [0005] Acid inhibition or rancidity often occurs in the anaerob...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12N1/02C12P5/02
CPCC12N1/02C12N1/20C12N1/36C12P5/023Y02E50/30
Inventor 李颖袁振宏孙永明孔晓英李连华王忠铭
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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