14 results about "Bioaugmentation" patented technology

The present application relates to the field of wastewater treatment, and particularly relates to a method for advanced denitrification of low carbon-nitrogen ratio wastewater based on short-cut nitrification and denitrification and anaerobic ammonia oxidation, which establishes an activated sludge bioaugmentation system by adding biochar immobilized ZB403 strain into an SBR activated sludge system, and domesticates the activated sludge bioaugmentation system by adjusting the component and content of simulated wastewater and optimizing the operation conditions of the bioaugmentation system, and optimizes the biological community structure in the system. The activated sludge bioaugmentation system is used for treating low carbon-nitrogen ratio wastewater, the inorganic nitrogen removal rate is above 99%, the COD removal rate is above 97%, and the sludge yield coefficient Yobs is 0.27-0.29 kgVSS / kgCOD.

Provided are the screening and identification of a Pseudomonas sp. strain for producing feruloyl esterase at high yield and a use of the Pseudomonas sp. strain as a bioaugmentation agent in fermented foods, relating to the technical field of brewing. By means of screening, a Pseudomonas sp. strain A-4H for producing feruloyl esterase at high yield is obtained. After cultivation for 36 h, the Pseudomonas sp. strain A-4H can produce ferulic acid at a concentration of 153.79±2.36 mg / L, and the activity of feruloyl esterase in a fermentation broth reaches 136.63±10.27 U / L. The addition of the Pseudomonas sp. A-4H during the brewing of Huangjiu can effectively increase the content of ferulic acid, the content of phenolic acid, and the content of organic acid in Huangjiu, providing an effective biological pathway for increasing the content of ferulic acid in fermented foods.

The invention relates to the technical field of aerobic treatment biology, in particular to an aerobic treatment biological synergy method which comprises the following steps: carrying out preliminary sedimentation treatment on sewage or organic waste to be treated to obtain a culture medium raw material, introducing the culture medium raw material into a culture tank, and determining a sludge bearing capacity characteristic value; determining the input amount of compound bacteria based on the characteristic value of the sludge bearing capacity and carrying out aerobic fermentation; in the aerobic fermentation process of the biological bacteria, determining the fermentation rate based on the oxygen consumption in the culture tank to obtain a fermentation rate change curve, and determining that the fermentation process enters an attenuation period based on the fermentation rate change curve; detecting the concentration of the compound bacteria, determining to increase the fermentation temperature and ventilation quantity based on the concentration of the compound bacteria, and continuing fermentation; and carrying out solid-liquid separation on the fermented composite flora mixed solution in the culture tank to obtain a high-concentration bacterial solution, and putting the high-concentration bacterial solution into a target sewage treatment system. According to the invention, the problems of low fermentation efficiency and insufficient activity of the composite flora in the prior art are solved.

The invention discloses an application of a rice OsPHR2 gene in regulation and control of selenium bioaugmentation. The rice OsPHR2 gene is shown as SEQ ID No: 1 in a sequence table, and the amino acid sequence of the protein of the rice OsPHR2 gene is shown as SEQ ID No: 2. According to the invention, the promoter of the rice OsPHR2 is adopted to drive the rice OsPHR2 to increase one to two copy numbers in the rice body, so that the content of phosphorus in the rice body is increased without influencing the growth, development and yield of the rice by means of moderately increasing the transcription expression of the rice OsPHR2, meanwhile, the absorption and accumulation of selenium can be promoted, and the biological enrichment enhancement of rice selenium is realized.

The invention discloses a wastewater perfluorinated compound precursor control method based on anaerobic-aerobic coupling biological enhancement, which comprises the following process steps: S1, monitoring an anaerobic and / or anoxic biological unit in a wastewater treatment system to identify a hotspot unit needing to be regulated; s2, aiming at the identified hotspot unit, implementing one or more of the following coordinated regulation measures for regulation, including regulation of hydraulic retention time, regulation of carbon nitrogen ratio and addition of a specific microbial agent; and S3, introducing the effluent of the hotspot unit regulated and controlled in S2 into a subsequent aerobic activated sludge tank and / or a Fenton advanced oxidation system for treatment. According to the technical scheme, the concentration of the PFCs generated by precursor conversion is reduced by 70% or above compared with that before regulation, and the total load of the PFCs in the effluent of the whole wastewater treatment system is reduced by 65% or above. The whole process depends on optimization of existing process parameters and addition of cheap microbial agents, and the operation cost of the whole process is reduced.

The invention discloses a biological enhancement fungicide and application thereof in aquaculture wastewater treatment, and belongs to the technical field of wastewater treatment microorganisms. The biologically-enhanced microbial agent is composed of five microorganisms, namely pseudomonas stutzeri, denitrified tropobelella, lysinibacillus fusiformis, paenibacillus polymyxa and compost bacillus, has the capacity of effectively removing chemical oxygen demand, ammonia nitrogen, total phosphorus, suspended solids, chloride ions and the like of the breeding wastewater, and can improve the treatment efficiency of various pollutants in the wastewater; according to the invention, the use amount of PAC and PAM is reduced, the treated sludge has a good physical structure and good settling performance, the variety and number of species in the system, especially the variety and number of some beneficial microorganisms in the system, can be significantly increased, the composition of microbial communities is greatly enriched, and more stable and diversified microbial communities are formed. In addition, the sewage treatment cost and the like can be remarkably reduced by using the biological enhancement microbial agent.

The present application relates to the technical field of sewage treatment, and particularly discloses a rural black and odorous water point source sewage treatment process based on electrode filler biological reinforcement. The process uses a stainless steel cylindrical reactor with a heat preservation layer, a supporting layer composed of gravel and quartz sand is arranged at the bottom, active carbon fillers are filled above the supporting layer, a composite anode is embedded in the filler layer, active carbon fillers are also filled above the anode, an aeration pipe is arranged at the bottom of the upper filler area, a stainless steel mesh cathode with a surface sprayed polyaniline layer is arranged to float on the water surface and is connected to an external power supply; the composite anode is modified by transition metal and carbon co-doping to strengthen the conductivity and catalytic activity, and the active carbon fillers are modified by iron and sulfur loading, and have the functions of adsorption and autotrophic denitrification. The process couples electrode embedding and filler modification to strengthen the electron transfer efficiency, activate the free radical mediated pollutant conversion path, and simultaneously improve the removal efficiency of carbon, nitrogen, phosphorus and antibiotics, and is suitable for rural domestic sewage treatment.

The invention relates to the technical field of microorganisms, and particularly discloses an acetobacter xylinum HNNB6-Se capable of resisting selenium and producing red selenium-rich nano cellulose, and the preservation number of the acetobacter xylinum HNNB6-Se is GDMCC (China General Microbiological Culture Collection Center) 66552. The strain is obtained by performing sodium selenite gradient stress domestication on an original strain acetobacter xylinum HNNB6, and can grow in a culture medium containing 1-200 [mu] g / mL of sodium selenite and synthesize the red selenium-rich nano bacterial cellulose. The selenium content in the produced nano cellulose (BC) reaches 50-5000 micrograms per gram by dry weight, and the produced nano cellulose (BC) is in stable red (the chromatic value a * is 0.5-25). The red selenium-rich nano bacterial cellulose has a selenium bioaugmentation function, and has outstanding application value in functional food selenium supplements (such as oral tablets and gel foods), medical materials (such as wound dressings) and beauty makeup products.

This invention relates to the field of wastewater treatment technology, specifically to a water purification device based on periclump bio-enhanced removal of antibiotics from water. It includes a swirling channel, a solar-powered PV / T coupled photothermal catalytic module, a periclump bioreactor, and a membrane water separation component. The front end of the swirling channel is connected to an inlet pipe, and the top effluent is connected to the PV / T coupled photothermal catalytic reaction module via a pipe. A set of water passages is located between the solar cell and the flat-plate collector at the bottom of the swirling channel. Wastewater treated by photothermal catalysis enters the periclump bioreactor below through these water passages. The bottom effluent from the periclump bioreactor is discharged after passing through the membrane water separation component. This invention optimizes the photothermal catalytic process and thermal energy conversion efficiency through solar PV / T splitting technology, coupled with micro / nano bubble technology, enhanced periclump biotechnology, and membrane water separation technology, achieving efficient removal of antibiotics and synergistic control of microbial resistance genes, effectively reducing the formation and horizontal migration of resistance genes.

This invention discloses a sludge increment method based on bioaugmentation in wastewater treatment processes, relating to the field of wastewater biological treatment technology, aiming to fill the gap in existing sludge increment methods based on bioaugmentation in wastewater treatment processes. The key technical points of this invention include: collecting wastewater treatment sludge; using a HiCS process to enrich, separate, and purify sludge increment bioaugmentation strains under alternating saturation-starvation environments; verifying and screening the efficacy of the sludge increment bioaugmentation strains based on the tCOD and sCOD concentrations in the sludge increment bioaugmentation strain culture system to obtain candidate strains for sludge increment bioaugmentation microbial communities; identifying the candidate strains and further screening them to construct a binary composite sludge increment bioaugmentation microbial community composed of two candidate strains from different species in an optimal ratio; and incrementing sludge based on the obtained bioaugmentation microbial community.

The invention discloses a two-stage EBPR / PN-EPDA synchronous nitrogen and phosphorus removal process based on biological enhancement. In the first stage, an enhanced biological phosphorus removal process is adopted, phosphorus accumulating bacteria are enriched through anaerobic / aerobic alternate operation, polyhydroxyalkanoate is synthesized by the phosphorus accumulating bacteria to serve as an internal carbon source, and efficient phosphorus removal and organic matter removal are achieved. In the second section, a short-cut nitrification-endogenous short-cut denitrification coupled anaerobic ammonia oxidation reactor is constructed, and deep denitrification is realized through anaerobic / aerobic / anoxic operation. The process is characterized in that sludge in the EBPR reactor is introduced into the PN-EPDA reactor for biological enhancement; on one hand, PHAs in the EBPR sludge is used for driving endogenous short-cut denitrification to generate nitrite; on the other hand, the ammonia oxidizing bacteria in the sludge are used for remolding microbial communities, inhibiting nitrite oxidizing bacteria and stabilizing short-cut nitrification. The process effectively solves the problem of insufficient carbon source in low-carbon sewage, can realize deep nitrogen and phosphorus removal without an additional carbon source, and reduces the cost.

The present application relates to wastewater treatment technical field, disclose a kind of based on biological reinforcement carrier fluidized bed biofilm's refining wastewater treatment device, including processing box, water inlet pipe is provided at the top of processing box, fluidized bed reactor is provided at the bottom of processing box, feeding pipe is provided at the top of fluidized bed reactor, water outlet pipe is provided at the bottom of fluidized bed reactor, communication pipe is provided at the bottom of processing box, and communication pipe is communicated with processing box and fluidized bed reactor.The based on biological reinforcement carrier fluidized bed biofilm's refining wastewater treatment device, by the aperture through-hole matching structure, can be targeted to generate the differentiation bubble of different sizes, adapts the complex oil stain component of refining wastewater.Small aperture second through-hole can output fine micro-bubble, can accurately adsorb emulsified oil, trace dissolved oil difficult to separate in water body, improve the light oil stain floating efficiency;Large aperture first through-hole can generate large volume bubble, efficiently wrap and carry large particle floating oil and massive oil stain in water body.

The present application relates to sewage treatment technical field, especially to a kind of coupling low temperature concentration and biological strengthening's denitrification sewage treatment system, including low temperature concentration section and biological strengthening section, biological strengthening section is composed of aerobic tank and anoxic tank, three fixed plates are fixedly connected in the inner wall of aerobic tank one side, three sliding plates are arranged in the inner wall of aerobic tank other side;Also involved a kind of coupling low temperature concentration and biological strengthening's denitrification sewage treatment system's use method, by the setting of adjusting plate, straight plate, F-shaped frame and adjusting mechanism and other structures, when needing to adjust the residence time of sewage in aerobic tank, the vertical adjusting plate is quickly turned into horizontal state, and then the sewage passing between adjusting plate and fixed plate and sliding plate, will be changed from original straight flow into S-shaped flow path, to extend the treatment reaction time of sewage in aerobic tank, while increasing the side aeration of partition frame, and the original bottom aeration assembly structure in aerobic tank, more dead angle-free, improve the treatment effect of device.

The invention relates to the technical field of aquaculture tail water treatment, in particular to a selective biological enhancement SBTS treatment system and process based on two-water synergy, the system comprises an in-situ remediation module, a terminal treatment module and a two-water synergy module, and the in-situ remediation module comprises an in-pond enhancement treatment module and an out-pond biological enhancement unit; the traditional three-pond and two-dam transformation adopts functional biological ropes with different functions to construct a biological array, microbial communities with different functions are respectively and directionally enriched, and aquatic water purification and water quality regulation and control are cooperatively realized; through the differentiated design of daily treatment operation and centralized drainage treatment, the process drainage is recycled after being treated, and the recession water is deeply purified by the tail end micro-flocculation contact filter and then is discharged after reaching the standard. The system is efficient and comprehensive in removal, high in impact load resistance, high in water resource utilization rate, low in operation cost and capable of stably meeting the breeding tail water discharge requirement, and a practical and effective technical path is provided for green and healthy breeding.