Preparation method and application of microbial inoculant for remediating poly-halohydrocarbon-polluted soil

A technology of polyhalogenated hydrocarbons and polluted soil, applied in the field of soil remediation in environmental engineering, can solve the problems of limiting the large-scale application of bioaugmentation technology and high cost, and achieve the effect of low cost, low content of heavy metals, and wide sources

Inactive Publication Date: 2013-10-02
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the current bioaugmented remediation process, the main problems are: the cost of the co-metabolism matrix, nutrients, biosurfactants and biological agents used in the implementation of the p...

Method used

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  • Preparation method and application of microbial inoculant for remediating poly-halohydrocarbon-polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] [1] Preparation of residual sludge lysate

[0028] Take the remaining sludge from the urban sewage treatment plant, adjust its concentration to 30g / L, control the initial pH at 11, control the pyrolysis temperature at 120°C, and control the pyrolysis time at 0.5 hours. Centrifuge the sludge lysate obtained above at 4000r / min After 30 minutes, collect the sludge lysate supernatant, and recover the precipitate.

[0029] [2] Culture substrate optimization

[0030] Divide the sludge cracking supernatant obtained in step [1] into two parts of equal volume, adjust the pH of one part to 2.0, let it stand for 18 hours, recover the sediment, and combine the remaining supernatant with another part of sludge cracking After the serum was mixed, an optimized culture substrate was obtained for the cultivation of degrading bacteria.

[0031] [3] Bacteria preparation

[0032] First, the quinone-reducing facultative anaerobic bacteria Shewanella oneidensis MR-1 (ATCC 700550) was inoc...

Embodiment 2

[0036] [1] Preparation of residual sludge lysate

[0037] Take the remaining sludge from the urban sewage treatment plant, adjust its concentration to 30g / L, control the initial pH at 11, control the pyrolysis temperature at 90°C, and control the pyrolysis time at 2 hours. Centrifuge the sludge lysate obtained above at 6000r / min After 20 minutes, the sludge lysed supernatant was collected, and the precipitate was recovered.

[0038] [2] Culture substrate optimization

[0039] Divide the sludge cracking supernatant obtained in step [1] into two parts of equal volume, adjust the pH of one part to 2.0, let it stand for 24 hours, recover the sediment, and combine the remaining supernatant with another part of sludge cracking After the serum was mixed, an optimized culture substrate was obtained for the cultivation of degrading bacteria.

[0040] [3] Bacteria preparation

[0041] First, the quinone-reducing facultative anaerobic bacteria Shewanella putrefaciens (1A02627) was ino...

Embodiment 3

[0045] [1] Preparation of residual sludge lysate

[0046] Take the remaining sludge from the urban sewage treatment plant, adjust its concentration to 30g / L, control the initial pH at 12, control the pyrolysis temperature at 115°C, and control the pyrolysis time at 1 hour, and centrifuge the sludge lysate obtained above at 4000r / min After 30 minutes, collect the sludge lysate supernatant, and recover the precipitate.

[0047] [2] Culture substrate optimization

[0048] Divide the sludge cracking supernatant obtained in step [1] into two parts of equal volume, adjust the pH of one part to 2.0, let it stand for 18 hours, recover the sediment, and combine the remaining supernatant with another part of sludge cracking After the serum was mixed, an optimized culture substrate was obtained for the cultivation of degrading bacteria.

[0049] [3] Bacteria preparation

[0050] First, the quinone-reducing facultative anaerobic bacteria Shewanella oneidensis MR-1 (ATCC 700550) was inocu...

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Abstract

The invention relates to a preparation method and application of a microbial inoculant for remediating poly-halohydrocarbon-polluted soil, belonging to the technical field of soil remediation in environmental engineering. The invention is characterized in that the microbial inoculant containing the quinone-reducing facultative anaerobe Shewanella sp. and poly-halohydrocarbon dehalogenated product facultative aerobic degrading bacterium is prepared by surplus sludge lysis solution preparation and culture medium optimization, and is used for remediating poly-halohydrocarbon-polluted soil. The microbial inoculant has the advantages of wide raw material sources, simple preparation technique and no secondary pollution during soil remediation, can effectively overcome the defect that the high operating cost of the biologically reinforced remediation of poly-halohydrocarbon-polluted soil limits the large-scale application, and has wide application prospects in remediation of soil polluted by poly-chlorine organic substances, poly-bromine aromatic hydrocarbons and poly-fluorine organic substances.

Description

technical field [0001] The invention belongs to the technical field of soil remediation in environmental engineering, and relates to a preparation method and application of a bacterial agent for remediating polyhalogenated hydrocarbon-contaminated soil. Background technique [0002] Most polyhalogenated hydrocarbons, such as polychlorinated organic compounds, brominated flame retardants, perfluorinated organic compounds, etc., as a typical class of persistent organic pollutants, mostly have "three-effect" effects, can interfere with the endocrine system of the human body, and in Widely present in various environmental media and living organisms worldwide, it has attracted widespread attention. They tend to accumulate in organisms and organic matter in soil / sediments, are not easy to degrade naturally, and cause persistent pollution to the environment. Therefore, the remediation of polyhalogenated hydrocarbon polluted environment has important theoretical and practical value...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 王竞路凯文马岩吕红柳广飞金若菲周集体
Owner DALIAN UNIV OF TECH
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