547 results about "Lysis" patented technology

The invention relates to the technical field of microorganisms, in particular to nocardia seriolae bacteriophage as well as a composition and application thereof. The name of the nocardia seriolae bacteriophage is RDP-NS002, the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number of the nocardia seriolae bacteriophage is CGMCC No.46674. The nocardia seriolae bacteriophage is named as RDP-NS002, and the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing nocardia seriolae; (2) preparing a product for inhibiting nocardia seriola; and (3) application in preparation of a product for preventing and / or treating fish nocardia disease caused by nocardia seriolae. The phage has the characteristics of high thermal stability, high acid-base stability and high splitting rate, has a long-acting inhibition effect, and is beneficial to prevention and treatment of nocardia seriola diseases.

The present application relates generally to the field of biochemical detection. The invention provides a method and a kit for detecting an anti-IgE antibody in a biological sample. The method for detecting the anti-IgE antibody in the biological sample comprises the following steps: (a) contacting the biological sample with an acidolysis solution to dissociate the anti-IgE antibody from IgE so as to obtain an acidolysis sample; (b) contacting the acidolysis sample with a neutralization buffer solution to terminate acidolysis so as to obtain a to-be-detected sample; and (c) determining the anti-IgE antibody in the sample to be detected. The kit for detecting the anti-IgE antibody in the biological sample comprises an acidolysis solution and a neutralization buffer solution. The problem of background IgE interference in anti-IgE antibody detection is solved to a certain extent.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention relates to the technical field of biological detection, and discloses a mycoplasma pneumoniae nucleic acid rapid detection method, which comprises the following steps: mixing a sample to be detected with a multifunctional pretreatment buffer solution containing tris (hydroxymethyl) aminomethane, potassium acetate, polyethylene glycol, saponin, spermine tetrahydrochloride and tris (2-carboxyethyl) phosphine hydrochloride, and carrying out cracking treatment; a cracking mixed solution is obtained; redissolving the enzymatic isothermal amplification basic reagent, the specific primer and the probe to obtain a redissolving amplification system; sucking the supernatant of the cracking mixed solution, adding the supernatant into a redissolving amplification system, and adding a magnesium acetate solution to obtain a reaction mixed solution to be detected; and carrying out isothermal amplification and signal acquisition to obtain a detection conclusion. The multifunctional pretreatment buffer solution is used for cracking the to-be-detected sample, sample liquefaction, cracking and nucleic acid release are completed in a single system, the nucleic acid extraction and purification step of centrifugal column adsorption or magnetic bead elution is avoided, and the operation process is simplified.

The invention discloses a vibrio cantoniensis bacteriophage vBVcaSR45Z and an application of the vibrio cantoniensis bacteriophage The invention relates to Vibrio campbelli phage vBVcaSR45Z, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is Courtyard 100 #, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO. 67441-B1. The invention further discloses a preparation method of the Vibrio campbelli phage vBVcaSR45Z strain. The bacteriophage vBVcaSR45Z has the advantages of rapid splitting capacity, high host inhibition effect, safe genome structure and clear systematic classification status, and provides an important basis for the bacteriophage vBVcaSR45Z as a biological prevention and control factor of aquatic vibrio pathogens.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an integrated micro-fluidic card box. The device comprises a first solution storage part, an adsorption part, a second solution storage part and a reaction part, the bottom surface of the first solution storage part is rotationally connected with the top surface of the adsorption part, the bottom surface of the adsorption part is rotationally connected with the top surface of the second solution storage part, and the bottom surface of the second solution storage part abuts against the top surface of the reaction part; a second channel penetrates through the first solution storage part, a second runner is arranged on the adsorption part, and a second through hole penetrates through one end of the adsorption part; during single-sample nucleic acid detection, lysate feeding, cleaning liquid feeding, eluent feeding and sample loading share an exhaust channel formed by the second flow channel and the second channel, and the flow process of the solution can be controlled only by one driving pump; the driving pump and the external pipeline only discharge gas but not liquid, so that the solution is prevented from being remained when passing through the pump and the pipeline, and pollution is avoided; a plurality of micro-fluidic chips are communicated with the driving pump and the controller, so that a plurality of samples can be detected at the same time, and the detection efficiency is improved.

The invention belongs to the technical field of substance extraction, and relates to a preparation method of PDRN with soothing and anti-wrinkle effects and a product and application of the PDRN with soothing and anti-wrinkle effects, the preparation method comprises the following steps: mixing a strain with a culture medium containing an accelerant, and fermenting to obtain fermentation liquor; centrifuging the fermentation liquor to take bacterial sludge, and mixing and cracking the bacterial sludge with a cracking solution to obtain a cracking product; carrying out mixed enzymolysis on the cracking product and a compound enzyme to obtain an enzymolysis product; centrifuging the enzymolysis product, mixing the obtained supernate with a DNA stabilizer, and performing high-temperature denaturation to obtain a denatured product; and centrifuging the denatured product, taking supernate, and filtering with a filter membrane to obtain the product. According to the preparation method disclosed by the invention, the use of an organic solvent is avoided, the stability of nucleic acid can be improved by adding the DNA stabilizer, and the prepared PDRN has excellent soothing and anti-wrinkle effects.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention discloses a high-flux and rapid chromatin immunoprecipitation sequencing method based on a HiBiT tag and a Tn5 enzyme, and belongs to the technical field of biology. According to the method, the high affinity characteristic of a HiBiT tag system is combined with a Tn5 enzyme in-situ library building technology, firstly, a DNA binding protein recombinant expression strain containing a HiBiT tag is constructed and subjected to induced expression, after formaldehyde crosslinking and cell lysis, an Anti-HiBiT antibody is used for rapid immunoprecipitation, and a Tn5 enzyme library is constructed; tn5 transposase is directly loaded on the magnetic beads combined with the protein and DNA fragment compound to complete fragmentation and linker connection, so that the construction of a next-generation sequencing library is quickly carried out, and the total consumed time from immunoprecipitation to the completion of library construction is less than or equal to 5 hours. Meanwhile, automatic treatment is realized through a micro experiment board (the reaction system is less than or equal to 200 microliters) and a liquid workstation, the experiment cost and the sample dosage are reduced, 48 transcription factors and 48 control samples can be synchronously treated, the efficiency is greatly improved, and an efficient tool is provided for microbial transcription regulation and control research.

A lysis capsule for performing a cell lysis procedure includes a hollow body having an open first end and an open second end, a first porous membrane covering the open first end, and a second porous membrane covering the open second end, and the hollow body defines a lysis chamber between the first and second porous membranes. A plurality of non-magnetic beads and at least one magnetic element are disposed within the lysis chamber, and the pores of the first and second porous membranes are sized to retain the non-magnetic beads and the magnetic element within the lysis chamber. An internal control may be contained within the lysis chamber to validate an assay result and / or to validate the effectiveness of the cell lysis procedure. The lysis capsule may be disposed within a sample chamber of a fluidic cartridge.

The invention discloses a bubble self-stratification digital detection method and system for miRNA quantification of extracellular vesicles, and belongs to the technical field of biomedical engineering and molecular diagnosis. The technical problem to be solved is to provide a simple and sensitive tumor extracellular vesicle miRNA detection method capable of simultaneously detecting various miRNAs. According to the scheme, a to-be-detected sample and immunocapture bubbles coupled with an anti-EpCAM antibody are incubated to enrich tumor extracellular vesicles, a to-be-detected miRNA sample is obtained through in-situ ultrasonic lysis, then the to-be-detected miRNA sample, a fluorescence / DNA double-coding magnetic bead conjugate and the like are used for constructing an enzyme digestion system for enzyme digestion, multifunctional click glass microbubbles are added to capture unreacted magnetic beads, standing is conducted, self-stratification is conducted, bottom magnetic beads are collected, and the EpCAM antibody is obtained. After fluorescence imaging, an image is input into the AI automatic fluorescence counting and decoding module for counting to obtain a result. The method is used for detecting miR-21 and miR-155 in tumor extracellular vesicles, and is suitable for noninvasive early diagnosis of tumors.

The invention relates to the technical field of bioengineering, in particular to a preparation process for extracting antihypertensive peptide from euphausia superba. The process comprises the following steps: adding a ferulic acid / arginine double-grafted pullulan compound in a preparation process, and breaking an emulsification interface by utilizing beta-cyclodextrin adsorption and phospholipase A2 cracking to obtain a soluble pretreatment product; meanwhile, short-time round-trip pH pulse is implemented in the incision enzyme hydrolysis process to activate potential enzyme cutting sites, finally, hydrophobic aggregation peptides are removed through weak acid rapid elution, and excision hydrolysis is performed to obtain a target product with the molecular weight of 0.5-3 kDa, so that dynamic induction and antioxidant steady-state dual control of hydrolysis reaction is realized; the generation proportion of short peptides and the ACE inhibition persistence are improved, and the flavor and the storage stability are remarkably improved. The method is controllable in technological process, high in repeatability and suitable for large-scale preparation of the euphausia superba protein functional peptide and development and application of antihypertensive food.

The present disclosure provides an in-vitro method of universal microbial lysis compatible with hybridization-based nucleic acid enrichment and extraction methods, comprising the steps of: (a) subjecting a sample to a combination of lysis steps to obtain a lysed sample, wherein the combination of lysis steps is selected from the group consisting of: (i) an ultrasound sonication, (ii) a combination of multiple sonication steps,- (iii) a combination of enzymatic treatment and one or more sonication steps, and (iv) a combination of enzymatic treatment, one or more sonication steps, and thermal step; and (b) treating the lysed sample of step (a) with one or more enzymes, followed by sonication for microbial lysis.

The invention relates to the field of gene detection, and particularly discloses a gene detection device and method applying Beidou satellite positioning, and the device comprises a sample collection and preprocessing module which is used for obtaining a body fluid or tissue biological sample of a user, and carrying out cell lysis, DNA / RNA extraction and purification operation on the sample; the gene sequencing analysis module is connected with the sample collecting and preprocessing module and is used for performing gene sequencing on the purified nucleic acid sample and identifying gene variation information related to tumors; the high-precision positioning capability of the Beidou system is utilized to obtain the environmental parameters of the geographic position of the user in real time, then the genetic variation data and the regional environmental carcinogenic factors are subjected to weighted fusion analysis through the risk assessment algorithm, and finally a highly personalized detection report is generated. In this way, the detection result can truly reflect the specific influence of the external environment on the individual health, and the accuracy and practicability of the report are remarkably improved.

The application discloses an Aeromonas hydrophila bacteriophage and application thereof, and the taxonomic name of the bacteriophage is Aeromonas hydrophila The application discloses an Aeromonas hydrophila bacteriophage and application thereof, and the taxonomic name of the bacteriophage is PTS0531-2, which is preserved in the Guangdong Microbial Culture Collection Center, and the preservation date is March 18, 2026, the preservation number is GDMCC No: 67971-B1, and the preservation address is No. 59, Block 5, 100, Martyrs' Road, Guangzhou. The Aeromonas hydrophila bacteriophage has a strong lysis effect on Aeromonas hydrophila derived from Chinese soft-shelled turtles, and can be used for preventing and controlling Aeromonas hydrophila in a Chinese soft-shelled turtle breeding environment or in vivo.

The invention relates to the field of gene mutation detection, and particularly discloses a POCT (point-of-care testing) detection method for V617F mutation of a human JAK2 gene. According to the method, an integrated detection tube is divided into a cell lysis-nucleic acid binding magnetic bead area, a nucleic acid cleaning area and a nucleic acid elution-amplification area from top to bottom in sequence through a plurality of hydrophobic layers, the cell lysis-nucleic acid binding magnetic bead area is pre-filled with a lysis solution, the nucleic acid cleaning area is pre-filled with a nucleic acid cleaning solution, and the nucleic acid elution-amplification area is pre-filled with a nucleic acid elution solution. A nucleic acid elution-amplification system is pre-installed in the nucleic acid elution-amplification area, in the detection process, only a sample needs to be added into the cell lysis-nucleic acid binding magnetic bead area, a corresponding instrument is inserted, and operation is conducted, so that nucleic acid extraction, amplification and result reporting can be completed, and additional manual processing steps are not needed. The method has the advantages of simplicity and convenience in operation, short time consumption, high sensitivity, strong specificity, low pollution risk and the like, can be directly used for whole blood sample detection, meets the rapid diagnosis requirements of clinical outpatient service, and is particularly suitable for auxiliary diagnosis of BCR-ABL negative myeloproliferative tumors.

Described herein is a singleplex RT-LAMP-based tests using modified primer sets. Contrived whole blood samples containing HIV-1 or HCV virions were diluted in equal parts water and loaded directly into optimized RT-LAMP master mixes. To mitigate cold-chain storage dependence, RT-LAMP reactions were performed using a lyophilized master mix. The reactions were heated for, for example 30 minutes using a hand-held, battery-powered heating device for simultaneous virion lysis and amplification.

The application belongs to the technical field of molecular biology, and particularly relates to a nucleic acid rapid extraction kit for transgenic detection of high-oil crops and application, and is particularly suitable for fluorescent quantitative PCR detection of transgenic components of high-oil crops. The kit comprises a lysis solution, a precipitation solution, a neutralization solution and an elution solution. The application can be completed within 15 minutes, effectively removes PCR inhibitors such as oil and protein, is specifically used for high-oil crops, and is a low-cost, safe and non-toxic nucleic acid rapid extraction method, and meets the requirement of on-site rapid detection of transgenic crops.

The application relates to the technical field of biology, and particularly relates to a giant panda coronavirus particle detection device and method. The device comprises a bottom plate, a driving element is fixedly connected to the top of the bottom plate, a main gear is coaxially fixedly connected to the output end of the driving element, an extension rod is coaxially fixedly connected to the top of the main gear, a centrifugal assembly for centrifuging a to-be-detected sample is arranged at the other end of the extension rod, a first spring is coaxially fixedly connected to the top of the main gear, the other end of the first spring is fixedly connected to the bottom of the centrifugal assembly, a first rotating shaft is fixedly connected to the top of the centrifugal assembly, and a liquid supplementing assembly for adding a lysis solution to the centrifugal assembly is arranged at the top of the first rotating shaft. The centrifugal assembly, the liquid supplementing assembly and the piston assembly are synchronously driven by the main gear and the driven gear, integrated operation of sample centrifuging and automatic addition of the lysis solution is realized, manual intervention is reduced to avoid sample pollution, the centrifuging process is not interrupted, and the detection pretreatment period is shortened.

The application discloses a device for extracting plasmid DNA in bacteria and relates to the technical field of biological medicine. The device comprises a first mixing assembly and a second mixing assembly; the first mixing assembly is connected with the second mixing assembly through a lysis spiral pipe; at least one liquid inlet is arranged on a connecting pipeline of the lysis spiral pipe and the second mixing assembly; the device has the advantages that the mixing assembly is connected in series, the lysis and neutralization processes are carried out in a closed environment, the probability of polluting the environment is reduced, the device is convenient to clean after use, continuous processing is realized, and the production efficiency is improved; and the device is simple, and the design and production cost are small.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention discloses a blocking agent for cell immunofluorescence detection by a slide method and a use method of the blocking agent. The blocking agent is a homologous no-load cell lysis solution without transfection plasmids, and the protein concentration is greater than or equal to 30mg / mL. The blocking agent is completely the same as cell cytoplasm and cell membrane components of cells to be detected except for plasmids-expressed antigens, when in use, the blocking agent is pre-mixed with a blood cleaning sample in advance, interfering antibodies except target antibodies in serum can be purified, and meanwhile, due to the fact that no antigen protein expressed after plasmid transfection exists, the blocking agent can be used for detecting the cells to be detected. And the protein concentration is equivalent to the globulin concentration in serum, so that the target antibody cannot be lost in the serum, and the slide method CBA detection sensitivity can be greatly improved.

The embodiment of the invention discloses a heavy chain variable region and a light chain variable region of an anti-GPC3 monoclonal antibody and application of the heavy chain variable region and the light chain variable region. The high-affinity anti-GPC3 sequence is obtained through screening, the binding specificity of the high-affinity anti-GPC3 sequence and the GPC3 antigen is high, and dissociation is slow. The sequence is used as an extracellular targeting domain to construct GPC3-CAR, after macrophages are transduced through lentivirus, CAR-M can specifically recognize GPC3 positive target cells, activate intracellular signal channels and remarkably improve in-vitro phagocytosis and killing activity, the hepatoma cell lysis effect is better, and technical support is provided for anti-hepatoma application.

A fluid sample is dispensed into a sample chamber of a fluidic cartridge, the sample chamber containing non-magnetic beads, a magnetic element, and an internal control reagent contained within an internal control pellet including an internal control for validating an assay result and / or to validate the effectiveness of a cell lysis procedure. The magnetic element is exposed to a magnetic field, thereby causing movement of the magnetic element, which causes movement of the non-magnetic beads. Movement of the non-magnetic beads causes cells contained within the fluid sample to lyse and release nucleic acids. The internal control reagent dissolves in the presence of the fluid sample or movement of the non-magnetic beads disintegrates the internal control pellet, thereby releasing the internal control into the fluid sample. Movement of the magnetic element and the non-magnetic beads causes the internal control to be distributed within the fluid sample.

The utility model relates to the technical field of cooling, in particular to a circulating cooling device for polypeptide cracking. According to the technical scheme, the heat exchange mechanism comprises a fixed frame, heat dissipation fins are fixedly installed in the center of the fixed frame, a fan is rotationally connected to the back of the fixed frame, a bimetallic coil spring piece is fixedly installed on the side of the fixed frame, the end of the bimetallic coil spring piece is bent, and a gap exists between the end of the bimetallic coil spring piece and the fixed frame; and the top of the fixed frame is rotationally connected with a knob electrically connected with the fan. When the environment temperature is high, the cooling effect of the cooling fins on the cooling medium is reduced, the high temperature of the cooling medium can cause that the internal temperature of the reaction kettle cannot be controlled, and at the moment, the cold-heat exchange efficiency of air and the cooling medium is accelerated by increasing the rotating speed of the fan and increasing the air circulation speed at the cooling fins; the environment temperature is prevented from affecting the heat dissipation effect of the cooling device.

The invention discloses a vibrio canetinii bacteriophage capable of being split across species and application, the bacteriophage is a vibrio canetinii bacteriophage CP2 which is preserved in the China Center for Type Culture Collection with the preservation number of CCTCCM20251108, and the vibrio canetinii bacteriophage CP2 is preserved in the China Center for Type Culture Collection. The vibrio cantoniensis bacteriophage CP2 is a virulent bacteriophage separated from the nature, is free of genetic modification, has high safety and strong application potential, has an optimal infection complex number of 1: 10000, has a fermentation titer of 3.5 * 10 < pfu > / mL after 12 h, can rapidly split vibrio cantoniensis, has a recognition rate of 96.7% to 180 vibrio cantoniensis strains and a splitting rate of over 96%, and can be used for preparing the vibrio cantoniensis bacteriophage CP2. The genome does not contain toxicity and bad genes, the biological safety is high, the bacteriophage is excellent in environmental adaptability, stable in activity when the pH value is 6-8, high in storage stability at 4-25 DEG C, capable of being stored for 12 months at 4 DEG C, capable of stably surviving at room temperature, tolerant to a common chemical agent povidone-iodine for aquatic products, and suitable for various breeding scenes. Meanwhile, the cracking spectrum is wide, and the cross-species capability is realized.

This utility model relates to the field of life science technology, and in particular to a nucleic acid extraction device. The device includes a frame, a sample loading and waste liquid treatment mechanism, a oscillating magnetic heating mechanism, a control device, and a screen interaction system. The sample loading and waste liquid treatment mechanism achieves precise three-dimensional positioning through horizontal and vertical motion guides and vertical moving components. It includes parallel large and small pistons, allowing for flexible switching between different volume pipetting and sharing a common connector. The oscillating magnetic heating mechanism integrates oscillation, heating, and magnetic attraction functions, and works with various sizes of pipette tips and reagent kits to complete sample processing. The extraction process is automated through the control device, linking all mechanisms to automatically complete the entire process, including sample lysis, solid-liquid separation, magnetic bead purification, and elution. This application automates nucleic acid extraction, improving operational efficiency and extraction results, and is suitable for nucleic acid extraction from plant tissues, animal tissues, blood, plasmids, and other samples.

Chimeric antigen receptors (CARs) with binding domains derived from a novel suite of human CD33-binding antibodies are described. The CARs include optimized short and intermediate spacer regions. The current disclosure also provides methods of cell expansion / activation processes utilizing IL-2, IL-7, IL-15, and / or IL-21 that improve cellular proliferation and cell lysis of the CARs as described.

The present disclosure relates generally to compositions, systems, and methods for lysing blood samples comprising an infectious agent, for example a bacteria, or a fungus, while maintaining viability of the infectious agent for subsequent characterization, for example phenotyping or genotyping. A lysing composition may include one or more surfactants, such as one or more detergents. A lysing composition may include lysing agent including one or more salts. A lysing composition may include one or more enzymes to break down one or more lyses byproducts, such as nucleic acids and / or protein aggregates.

This invention relates to DNA extraction methods from soil and sediments with high humic substances content. The method focuses on the soil preparation phase; removing salts and cations with two different Tris-EDTA (TE) buffers, followed by precipitating humic substances with aluminum hydroxide solution and washing again with TE and cell lysis phase; urea is used as denaturing agents for the cell lysis phase and protein degradation phase while preventing the rediffusion of humic substances with CTAB. The purification can be performed following both the classic phenol chloroform method and using commercial DNA purification columns. This method is very accessible with operation time varying from 1h40 min to 2h30 min. The invention involves the use of TE and aluminum hydroxide solutions for soil preparation, and urea and CTAB as denaturing agents during cell lysis, in the specified concentrations, order, and incubation time, to prevent contamination of final DNA with humic substances