1185 results about "Lysis" patented technology

The present disclosure relates to methods, compositions and systems for droplet processing. For example, methods can include (a) providing a first emulsion comprising a first droplet population, wherein droplets of the first droplet population comprise a lysis reagent, and a second emulsion comprising a second droplet population, wherein a droplet of the second droplet population comprises i) a cell or a nucleus, and ii) a plurality of nucleic acid barcode molecules; (b) subjecting the first emulsion and second emulsion to conditions sufficient to transfer the lysis reagent to the second droplet population via micelles comprising the lysis reagent; and (c) lysing the cell or the nucleus within the droplet of the second droplet population with the lysis reagent.

The present disclosure relates to methods, compositions and systems for droplet processing. For example, a method can include (a) partitioning a plurality of cells into a plurality of aqueous droplets, wherein an aqueous droplet of the plurality of aqueous droplets comprises a cell of the plurality of cells; and delivering a micellized lysis agent to the cell in the aqueous droplet.

The invention discloses an integrated fluorescence detection kit and a testing method thereof.The integrated fluorescence detection kit comprises an upper cover assembly, a lysate tube assembly, a main body assembly, a diluent tube assembly and a decorative cover, and the lower end of the upper cover assembly is connected with the lysate tube assembly and the diluent tube assembly; the side wall of the upper cover assembly is connected with the upper part of the main body assembly, and the main body assembly is nested in the decorative cover; a reagent system in the kit comprises an RT-RAA reaction system and a CRISPR reaction system which are stored in the detection kit at normal temperature in the form of freeze-dried microspheres and closed liquid, and a lysis solution and a diluent are released through the lysis solution tube assembly and the diluent tube assembly and react in the main body assembly. According to the invention, the problems that the existing fluorescence detection operation process is complicated, the instrument is not portable and depends on professional analysis and the like are solved, and the rapid, portable and user-friendly detection on the virus is realized based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) detection system.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

The present invention relates to a method for extracting exosome-like nanovesicles from Platycladus orientalis leaves by freeze-drying and enzymatic hydrolysis, and the extracted exosome-like nanovesicles and applications thereof. Specifically, the freeze-drying and enzymatic hydrolysis method for extracting exosome-like nanovesicles from Platycladus orientalis leaves provided by the present invention ensures a stable supply of raw materials, is easy to store for a long time, and significantly improves the extraction efficiency and economy, opening up a new path for the commercial production of exosome-like nanovesicles from plant sources. Through freeze drying and low-temperature enzymatic hydrolysis technology, the active ingredients in Platycladus orientalis leaves are retained to the maximum extent, and the fiber and polysaccharide colloid are effectively removed, thereby improving the purity and biological activity of the exosome-like nanovesicles. The method is simple, easy to operate, low in cost, and suitable for large-scale production. In addition, the present invention applies the extracted Platycladus orientalis leaves exosome-like nanovesicles to hair growth, especially for androgenic alopecia (AGA), providing a safe and efficient new treatment strategy, and is expected to lead a new trend in the preparation and application of plant exosome-like nanovesicles.

The invention relates to the technical field of microorganisms, in particular to nocardia seriolae bacteriophage as well as a composition and application thereof. The name of the nocardia seriolae bacteriophage is RDP-NS002, the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number of the nocardia seriolae bacteriophage is CGMCC No.46674. The nocardia seriolae bacteriophage is named as RDP-NS002, and the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing nocardia seriolae; (2) preparing a product for inhibiting nocardia seriola; and (3) application in preparation of a product for preventing and / or treating fish nocardia disease caused by nocardia seriolae. The phage has the characteristics of high thermal stability, high acid-base stability and high splitting rate, has a long-acting inhibition effect, and is beneficial to prevention and treatment of nocardia seriola diseases.

The invention belongs to the technical field of genetic engineering and biological medicine, and particularly discloses a cascade response type nano-particle with a microenvironment remodeling function and a preparation method and application of the cascade response type nano-particle. The nanoparticle provided by the invention adopts a bionic hybrid membrane engineering strategy: an inner core is formed by loading an exosome inhibitor on a nano material, and the surface is covalently coupled with M2 type macrophage specific targeting peptide; the outer layer coats a macrophage membrane and active oxygen response type liposome composite membrane layer through a co-extrusion technology, and is loaded with a TLR agonist. Animal experiments show that after being injected through caudal vein, the nano-particles are enriched at a tumor site in a targeted manner through homing receptors such as membrane surface integrin alpha4beta1 and the like, and outer membrane cracking is triggered in a high-ROS tumor microenvironment, so that space-time controlled release of a TLR agonist and targeted phagocytosis of an exosome inhibitor by macrophages guided by a targeted peptide are realized. The anti-tumor synergistic effect is achieved through multiple regulation and control mechanisms, and an effective treatment strategy is provided for tumor immunotherapy.

The present invention relates to the field of biomedicine and discloses a platelet-rich plasma for anti-aging and promoting tissue repair and regeneration, a preparation method, and an application thereof, comprising the following steps: obtaining concentrated platelet plasma, and treating the concentrated platelet plasma with low-intensity pulsed ultrasound; the treatment conditions are one of the following treatment methods: 1) the treatment power is 2 to 5 W / cm 2 , the pulse period is 50%, and the ultrasonic treatment time is 2~10 min; 2) First, the first ultrasonic treatment is performed: using 0.2~1.0 W / cm 2 , the pulse period is 50%, ultrasonic treatment is 1~5 min; then the second ultrasonic treatment is carried out: 1.5~5.0 W / cm 2 , the pulse period is 50%, and the ultrasonic treatment is 1 to 5 minutes; the present invention adopts a special ultrasonic treatment system to obtain a PRR product with special activation and lysis characteristics. The product has active substances that are beneficial to tissue repair and regeneration and anti-aging, and shows excellent effects in promoting cell proliferation, cell migration and wound healing.

The invention provides a method for simultaneously detecting various micro-plastics in water, and belongs to the technical field of micro-plastic detection. The method comprises the steps of sample pretreatment, thermal cracking-gas chromatography-mass spectrometer measurement, qualitative analysis and quantitative analysis. The method comprises the following steps: taking a water sample, uniformly shaking, filtering with a filter membrane, taking the filtered filter membrane, adding acetone, carrying out ultrasonic elution, washing the filter membrane, and collecting an eluent; adding acetone into the eluted filter membrane, carrying out ultrasonic elution again, washing the filter membrane, collecting the eluent, combining the two eluents, heating and concentrating in a water bath, adding acetone, ultrasonically mixing, transferring into a cracking cup, heating and volatilizing, and determining by a thermal cracking-gas chromatography-mass spectrometer. Carrying out qualitative analysis through analysis of cracking products with different types of micro-plastic characteristics, and then carrying out quantification through an external standard method; the method provided by the invention can accurately quantify the six microplastics in the effluent, and has the advantages of simple pretreatment, small sampling amount, high accuracy and lowest detection limit of 0.012 [mu] g / L.

The present application relates generally to the field of biochemical detection. The invention provides a method and a kit for detecting an anti-IgE antibody in a biological sample. The method for detecting the anti-IgE antibody in the biological sample comprises the following steps: (a) contacting the biological sample with an acidolysis solution to dissociate the anti-IgE antibody from IgE so as to obtain an acidolysis sample; (b) contacting the acidolysis sample with a neutralization buffer solution to terminate acidolysis so as to obtain a to-be-detected sample; and (c) determining the anti-IgE antibody in the sample to be detected. The kit for detecting the anti-IgE antibody in the biological sample comprises an acidolysis solution and a neutralization buffer solution. The problem of background IgE interference in anti-IgE antibody detection is solved to a certain extent.

The invention discloses a lytic bacteriophage for preventing and controlling vibrio in an aquaculture environment. The bacteriophage vBValMR42H belongs to a muscle tail bacteriophage and is insensitive to chloroform, the capsid of the bacteriophage vBValMR42H is free of lipid substances, and the preservation number is GDMCC No: 67434-B1. The bacteriophage has the characteristics of high adsorption speed, short incubation period and high cracking amount, keeps high activity in a wide range of temperature (4-55 DEG C) and pH (2-11), and has strong environmental adaptability. Genome analysis shows that the bacteriophage does not contain virulence factors, antibiotic resistance genes and lyogen related genes, and is high in biological safety. Physical development analysis shows that the method can be divided into a new genus. The bacteriophage R42H has the advantages of high splitting efficiency, good environmental stability, strong specificity, gene safety and the like, and can be used as an ideal biological prevention and control agent for preventing and controlling vibrio alginolyticus diseases in aquaculture.

The invention provides a method for detecting salmonella enteritidis, which comprises the following steps of: 1, expressing a nano antibody for resisting salmonella enteritidis FliC by using escherichia coli, and verifying the nano antibody; step 2, preparing immunomagnetic beads for resisting FliC protein, and verifying the immunomagnetic beads; step 3, carrying out specific enrichment on the target bacteria; 4, performing rapid splitting decomposition to release nucleic acid; step 5, carrying out isothermal amplification on the target DNA fragment; and step 6, CRISPR / Cas12a mediated signal amplification and detection are carried out. The kit has the effects of ultra-fast detection, high sensitivity, high specificity, portability, easiness in operation, no need of bacterial culture and the like.

The invention relates to the technical field of biological detection, and discloses a mycoplasma pneumoniae nucleic acid rapid detection method, which comprises the following steps: mixing a sample to be detected with a multifunctional pretreatment buffer solution containing tris (hydroxymethyl) aminomethane, potassium acetate, polyethylene glycol, saponin, spermine tetrahydrochloride and tris (2-carboxyethyl) phosphine hydrochloride, and carrying out cracking treatment; a cracking mixed solution is obtained; redissolving the enzymatic isothermal amplification basic reagent, the specific primer and the probe to obtain a redissolving amplification system; sucking the supernatant of the cracking mixed solution, adding the supernatant into a redissolving amplification system, and adding a magnesium acetate solution to obtain a reaction mixed solution to be detected; and carrying out isothermal amplification and signal acquisition to obtain a detection conclusion. The multifunctional pretreatment buffer solution is used for cracking the to-be-detected sample, sample liquefaction, cracking and nucleic acid release are completed in a single system, the nucleic acid extraction and purification step of centrifugal column adsorption or magnetic bead elution is avoided, and the operation process is simplified.

The invention discloses a method for extracting PN / PDRN from lactobacillus bulgaricus and application of the PN / PDRN in relieving and anti-inflammatory aspects, belongs to the technical field of biochemistry, and solves the problems that in the prior art, the acquisition source of PN / PDRN is unstable, and pollution easily exists. According to the preparation method, cell walls of lactobacillus bulgaricus are preliminarily dissolved through alkaline conditions and lysozyme in the preparation process, cell membranes of bacteria are destroyed through lauryl sodium sulfate to be split, the conditions are mild, meanwhile, PVP is added to protect a PN structure, and the purity of the PN / PDRN is effectively improved through a final product prepared through the preparation method.

The invention discloses a vibrio cantoniensis bacteriophage vBVcaSR45Z and an application of the vibrio cantoniensis bacteriophage The invention relates to Vibrio campbelli phage vBVcaSR45Z, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is Courtyard 100 #, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO. 67441-B1. The invention further discloses a preparation method of the Vibrio campbelli phage vBVcaSR45Z strain. The bacteriophage vBVcaSR45Z has the advantages of rapid splitting capacity, high host inhibition effect, safe genome structure and clear systematic classification status, and provides an important basis for the bacteriophage vBVcaSR45Z as a biological prevention and control factor of aquatic vibrio pathogens.

The disclosure discloses a liquid specimen lysis detection device. The device includes a detection unit, a lysis unit and a detachable limit structure, where the detection unit includes a detection shell, a sample acquisition unit and a detection reagent strip, the lysis unit includes a base, a piercing sleeve, a sealing film and a bottom bottle. The detachable limit structure is installed between the detection unit and the lysis unit to limit the depth of the detection unit inserted into the top opening of the base in the initial state, so as to prevent the piercing sleeve from piercing the sealing film. In the detection state, the detachable limit structure is removed, and the piercing sleeve pierces the sealing film under the push of the plug-in section of the detection shell; the sample acquisition unit extends into the bottom bottle.

The invention belongs to the technical field of waste PET (Polyethylene Terephthalate) recovery, and particularly relates to a continuous production system and process for efficiently recovering and refining PTA (Purified Terephthalic Acid) from waste PET and application. According to the continuous production system and process for efficiently recovering and refining the PTA from the waste PET, through mutual cooperation of the enzymolysis unit, the first solid-liquid separation unit, the filter cake dissolving and decolorizing unit, the electrolysis unit, the second solid-liquid separation unit and the recrystallization unit, continuous production of'obtaining the refined PTA by taking the waste PET as a raw material 'is realized; besides, by adjusting the runner structure in the electrolysis assembly of the electrolysis unit, the situation of electrolysis interruption caused by the fact that TA generated by electrolysis is prone to being deposited on the surface of the anode plate is avoided, guarantee is provided for continuous proceeding of the whole degradation and recovery process, and the system and the process have important significance on efficient degradation and recovery of waste PET.

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention discloses new application of Pseudomonas sp. F13, namely application of the Pseudomonas sp. F13 in splitting haematococcus pluvialis to extract astaxanthin, the Pseudomonas sp. F13 and the haematococcus pluvialis are jointly fermented and cultured, the Pseudomonas sp. F13 can split the haematococcus pluvialis, so that the astaxanthin in the haematococcus pluvialis is fully released, and the extraction rate reaches 76%. The method is simple and easy to operate, low in cost and suitable for industrial production. Solvents and wall breaking equipment are not needed in extraction, so that the negative influence on the environment is reduced, and the method is suitable for industrial production.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an integrated micro-fluidic card box. The device comprises a first solution storage part, an adsorption part, a second solution storage part and a reaction part, the bottom surface of the first solution storage part is rotationally connected with the top surface of the adsorption part, the bottom surface of the adsorption part is rotationally connected with the top surface of the second solution storage part, and the bottom surface of the second solution storage part abuts against the top surface of the reaction part; a second channel penetrates through the first solution storage part, a second runner is arranged on the adsorption part, and a second through hole penetrates through one end of the adsorption part; during single-sample nucleic acid detection, lysate feeding, cleaning liquid feeding, eluent feeding and sample loading share an exhaust channel formed by the second flow channel and the second channel, and the flow process of the solution can be controlled only by one driving pump; the driving pump and the external pipeline only discharge gas but not liquid, so that the solution is prevented from being remained when passing through the pump and the pipeline, and pollution is avoided; a plurality of micro-fluidic chips are communicated with the driving pump and the controller, so that a plurality of samples can be detected at the same time, and the detection efficiency is improved.

The utility model discloses a sample treatment and detection integrated device, and belongs to the field of medical examination and inspection instruments and molecular diagnosis and detection instruments. According to the technical key points, the system comprises a system outer cover assembly, a loading round chassis assembly and a rotary liquid transfer device assembly, the object-carrying circular chassis assembly comprises an object-carrying circular chassis; a waste liquid bin, a magnetic bead lysis solution bin, a reagent tube, a PCR tube and a washing liquid bin are uniformly arranged on the loading round chassis; an insertion cylinder is further arranged in the middle of the loading round base plate; the rotary liquid transfer device assembly comprises a rotary liquid transfer device; the offset pipette comprises an upper through pipe interface, a lower pipe interface and an offset pipe; the lower portion of the first vertical through pipe is connected with the upper through pipe connector, and the upper portion of the second vertical pipe is connected with the lower through pipe connector. The first vertical through pipe and the second vertical pipe are located on the same vertical axis, and the first vertical through pipe is communicated with the offset pipe.

The invention discloses a method for cooperatively controlling biological pollution of a reverse osmosis membrane by group quenching bacteria and bacteriophages. According to the invention, a QQ-bacteriophage carrying system composed of quorum quenching (QQ) bacteria and lysing bacteriophages is constructed, and the bacteriophages and the QQ bacteria are utilized to synergistically improve the inhibition effect on biofilm formation and biological pollution. In the QQ-bacteriophage carrying system, the bacteriophage can perform targeted lysis on core contaminating bacteria, and the QQ bacteria can degrade quorum sensing molecules, weaken an anti-bacteriophage defense system of the bacteria and promote movement and infection of the bacteriophage in a biological membrane. Therefore, the QQ-bacteriophage carrying system can remarkably improve the biological membrane removal efficiency, recover the membrane flux and prolong the service life of the reverse osmosis membrane. The invention can provide an efficient, green and sustainable biological pollution control solution for the fields of water treatment, seawater desalination, industrial wastewater reuse and the like.

The utility model relates to the technical field of enzymolysis tanks, in particular to a nitrogen-filled enzymolysis tank for seaweed extracts, comprising a tank body, the top of the tank body being detachably connected with a cover body, the inner bottom and top of the tank body being respectively fixedly connected with a lower air guide ring and an upper air guide ring, the outer side of the tank body being provided with a main manifold, nitrogen being sprayed out through a plurality of air guide holes, so that the nitrogen is respectively introduced from the bottom and the top of the tank body, the nitrogen is fully mixed with an enzymolysis solution through the plurality of air guide holes, the mixing effect is improved, and when a liquid guide pump is started at the same time, the enzymolysis solution at the bottom of the tank body enters the interior of a diverter plate through a liquid guide pipe, and then is sprayed out through a plurality of evenly distributed nozzles, so that the enzymolysis solution and the nitrogen above the tank body are further contacted and mixed, the mixing effect is good, so that the entire enzymolysis system starts temperature-controlled enzymolysis under the condition of being full of nitrogen protection, the unsaturated fatty acids in the enzymolysis solution are fully protected, the oxidation reaction is reduced, and the nutritional value of the active ingredients is retained.

The invention discloses a bioactive peptide with antioxidant and hypoglycemic effects, a preparation and application thereof, and belongs to the technical field of bioactive peptides. The bioactive peptide provided by the invention is as shown in at least one of SEQ ID NO: 1 to SEQ ID NO: 4. Degreased shiny-leaved yellowhorn meal is used as a raw material, after high-temperature heat treatment and ultrahigh-pressure pretreatment are performed, the bioactive peptide with antioxidant and hypoglycemic effects is separated through enzymolysis, ultrafiltration and a liquid chromatography separation technology in combination with a bioinformatics technology, and components and sequences of the bioactive peptide are preliminarily identified through a liquid chromatography-mass spectrometry technology. And carrying out chemical synthesis according to the identified peptide fragment sequence to obtain four bioactive peptides with antioxidant and hypoglycemic effects. Through detection, the peptide fragments and the compound peptide fragments thereof have good alpha-glucosidase and alpha-amylase inhibitory activity, and have good application prospects in preparation of products with antioxidant and hypoglycemic effects.

The invention belongs to the technical field of substance extraction, and relates to a preparation method of PDRN with soothing and anti-wrinkle effects and a product and application of the PDRN with soothing and anti-wrinkle effects, the preparation method comprises the following steps: mixing a strain with a culture medium containing an accelerant, and fermenting to obtain fermentation liquor; centrifuging the fermentation liquor to take bacterial sludge, and mixing and cracking the bacterial sludge with a cracking solution to obtain a cracking product; carrying out mixed enzymolysis on the cracking product and a compound enzyme to obtain an enzymolysis product; centrifuging the enzymolysis product, mixing the obtained supernate with a DNA stabilizer, and performing high-temperature denaturation to obtain a denatured product; and centrifuging the denatured product, taking supernate, and filtering with a filter membrane to obtain the product. According to the preparation method disclosed by the invention, the use of an organic solvent is avoided, the stability of nucleic acid can be improved by adding the DNA stabilizer, and the prepared PDRN has excellent soothing and anti-wrinkle effects.

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The invention relates to the technical field of medical health information, in particular to a medical infection control intelligent monitoring system based on the Internet of Things. The system comprises a rapid microorganism monitoring module, a multi-dimensional gating behavior analysis module, a space-time causal reasoning module and an intelligent intervention control module, and can be used for sampling and flushing air in an operating room to obtain enriched microorganism suspension; introducing the enriched microorganism suspension into a lysis chamber for cell lysis release to obtain an ATP release liquid; performing viable bacterium density quantification on the ATP release liquid to obtain a real-time bacterial colony density index; collecting a gating original data stream; identifying gating behavior characteristics of the gating original data stream; and performing passage scene semantization on the gating behavior characteristics to obtain a scene semantic tag. According to the invention, the normal form transformation of medical environment infection control from passive monitoring to active prevention is realized through medical informatics and data processing and computing technologies.

The invention relates to the technical field of life science, in particular to a full-automatic nucleic acid extraction system and process, and the system comprises a complete machine frame, a sample adding and waste liquid treatment mechanism, an oscillation magnetic suction heating mechanism, a control system and a screen interaction system. The sample adding and waste liquid treatment mechanism realizes three-dimensional accurate positioning through transverse and longitudinal movement guide rails and a vertical movement assembly, comprises a large piston device and a small piston device which are connected in parallel, and can flexibly switch different volumes of pipettes and share a connector; the oscillation magnetic suction heating mechanism integrates oscillation, heating and magnetic suction functions and is matched with multi-specification suction heads and kits to complete sample treatment. According to the extraction process, all the mechanisms are linked through a control system, and full-flow operation such as sample cracking, solid-liquid separation, magnetic bead purification and elution is automatically completed. The nucleic acid extraction device realizes automation and precision of nucleic acid extraction, improves the operation efficiency and the extraction effect, and is suitable for nucleic acid extraction of samples such as plant tissues, animal tissues, blood and plasmids.

The invention belongs to the technical field of biological membrane removal, and particularly relates to a method for removing a biological membrane on the surface of micro-plastic. Comprising the following steps that micro-plastic is added into an ethanol solution with the volume concentration being 30%-90% for cracking, and the adding amount of the micro-plastic is 20 g / L; carrying out ultrasonic treatment for 10-40 minutes after cracking, cleaning to remove a biological membrane which falls off after ultrasonic treatment, and drying after cleaning to obtain the micro-plastic without the biological membrane. The biological membrane on the surface of the micro-plastic is removed to the greatest extent under the condition that the physicochemical properties of the micro-plastic are not changed.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.