The invention discloses a
Chlorella zofingiensis heterotrophic high-density culture method which is characterized in that
Chlorella zofingiensis is inoculated into a
fermentation tank and cultured, aused basal culture medium and a used fed-batch culture medium take
ammonium salt as a
nitrogen source, the
carbon nitrogen ratio of the basal culture medium is 5:(1-80):1, the
carbon nitrogen ratio ofthe fed-batch culture medium is 5-20 times that of the basal culture medium, the pH (potential of
hydrogen) value of a culture
system is monitored in real time during culture, adjusted by adding
ammonia water according to monitoring results and kept 6.5+ / -0.2 to supplement the
nitrogen source, the glucose concentration of the culture
system is monitored in real time during culture, feeding of thefed-batch culture medium is started when the glucose concentration of the basal culture medium is reduced to 3-4 g / L, and the variation amplitude of the glucose concentration of the culture
system islower than 20% within any two hours by the aid of feeding speed. According to the characteristic of
pH reduction in the
ammonium salt using process of cells, the pH is regulated by the aid of the
ammonia water to supplement the
nitrogen source. The culture method can effectively avoid the
inhibitory effect of
nitrogen source excess or deficiency in an existing
fixed carbon nitrogen ratio feedingculture mode on
cell growth.