20results about How to "Low cytotoxicity" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The application discloses a drainage bag with an antibacterial functional layer and a preparation process thereof, and relates to the technical field of medical materials, and comprises the following steps: adding modified montmorillonite into N, N'-dimethylacetamide, uniformly mixing through ultrasonic, adding a polyurethane film, ultrasonic immersion for 60-120 min, drying, and obtaining a drainage bag substrate; adding polylactic acid into N, N-dimethylformamide, uniformly stirring and mixing, adding modified nano zinc oxide, uniformly mixing through ultrasonic, and then performing electrospinning to obtain an antibacterial coating fiber film; placing the antibacterial coating fiber film on the inner side surface of the drainage bag substrate, high-frequency heat sealing, assembling, and obtaining the drainage bag.

This invention discloses a weak force sensor based on a teardrop-shaped micro / nano optical fiber and its fabrication method. The sensor comprises a biconical micro / nano optical fiber, a single-conical optical fiber, a quartz capillary tube, and a PDMS microsphere. The biconical micro / nano optical fiber is symmetrically bent, with the central waist region fiber forming a teardrop-shaped ring. The two ends of the teardrop-shaped micro / nano optical fiber ring are sequentially connected to portions of the waist region fiber, a transition region fiber, and a standard fiber. This invention utilizes the sensitive self-sensing capability of micro / nano optical fibers to achieve integrated signal input and output, greatly simplifying the measurement feedback mechanism of the sensing system and reducing overall cost. Furthermore, it is simple and intuitive to operate, reducing the technical requirements for operators. In terms of sensor fabrication, the main raw materials are standard optical fiber and quartz capillary tube, both of which are extremely low-cost. Therefore, this micro / nano optical fiber sensor exhibits significant cost-effectiveness.

The application discloses an antibacterial peptide derived from the skin of bullfrog and application thereof, and the amino acid sequence of the antibacterial peptide is GSVGPVGPR (as shown in SEQ ID NO:1), and the molecular weight is 824.4504 g / mol. The antibacterial peptide has obvious inhibiting effect on Staphylococcus aureus, and can be used for preparing a medicine for preventing or inhibiting Staphylococcus aureus infection. Meanwhile, the antibacterial peptide and vitamin A have a significant synergistic bactericidal effect on Staphylococcus aureus. The antibacterial peptide and vitamin A can significantly reduce the use concentration of single component, improve the antibacterial efficiency, overcome the bacterial drug resistance problem, and can be used for preparing antibacterial medicines, skin external preparation, medical dressings, feed additives and food preservatives.

The application discloses an antibacterial peptide designed by a generative artificial intelligence framework and targeting gram-negative bacteria and an application thereof, and belongs to the fields of artificial intelligence and biotechnology. The application designs and successfully obtains a novel antibacterial peptide by a generative artificial intelligence framework, the antibacterial peptide targeting gram-negative bacteria and having good antibacterial activity. It is verified by experiments that the antibacterial peptide MTAMP003 has good bacteriostatic capacity on E. coli, S. typhimurium, P. aeruginosa and A. baumannii, exhibits low cytotoxicity on human gastric mucosa epithelium and low hemolyticity on rabbit red blood cells, and has good application prospect and research value.

This invention discloses a method for preparing a coumarin derivative and its application. The coumarin derivative is obtained by Perkin condensation and cyclization reaction of 2,4-dihydroxybenzaldehyde with ethyl acetoacetate to obtain compound (I). Compound (I) then undergoes a nucleophilic substitution reaction with 2,4-dinitrobenzenesulfonyl chloride to obtain the coumarin derivative, the chemical structure of which is shown in formula (II). The coumarin derivative (II) of this invention exhibits significant fluorescence responsiveness and high sensitivity to GSH in 0.2% DMSO-water solution, with a detection limit of 0.49 μM. Furthermore, this compound has low toxicity to H9c2 cells and can be used for the detection of GSH in live cells. This invention provides a simple and highly sensitive coumarin derivative for the detection of GSH, which has broad application prospects.

The application provides a fluorescent probe compound for simultaneously detecting HClO and SO2 at double sites and synthesis and application thereof. The probe compound takes phenothiazine as a response group of HClO, expands the conjugated structure of phenothiazine, and introduces a response group of SO2 and an organelle positioning group on the near-infrared fluorescent skeleton, thereby constructing an endoplasmic reticulum-targeted fluorescent probe BQTPA for simultaneously detecting HClO and SO2. The probe can realize dual-channel simultaneous detection of HClO and SO2 through two completely separated emission channels (λem=598 nm and 448 nm), and exhibits excellent selectivity, high sensitivity (detection limits are 7.9 nM and 33.8 nM, respectively) and good light stability to the response of HClO and SO2. The probe is successfully used for monitoring the changes of exogenous and endogenous HClO and SO2 levels in cells, further reveals the dynamic changes of the two in the process of drug-induced liver injury and ferroptosis, and clarifies the role of ferroptosis in liver injury.

This invention discloses an antimicrobial peptide, Cih1, and its applications. The amino acid sequence of antimicrobial peptide Cih1 is shown in SEQ ID NO.1. The antimicrobial peptide Cih1 provided by this invention is a cationic peptide, rich in cysteine ​​(-cys) residues, which can form an amphiphilic β-sheet secondary structure. This structure endows it with excellent antibacterial and antiviral activity, and can effectively inhibit the proliferation and invasion of pathogens in aquaculture water. Based on the broad-spectrum resistance of antimicrobial peptide Cih1 to pathogens, it can help aquatic animal hosts resist infection by various pathogens, and has broad application prospects in the field of anti-infective drug development for aquaculture.

This invention discloses a core-shell microneedle loaded with silver nanoparticles and growth factors, its preparation method, and its application, belonging to the field of biomedical technology. The core-shell microneedle includes a backing layer and an array of needle tips disposed on the backing layer; the needle tips in the needle tip array have a core-shell structure, with the shell layer of the needle tip loaded with protein-Ag2O2 composite nanoparticles and the core layer of the needle tip loaded with growth factors. The preparation method of the protein-Ag2O2 composite nanoparticles is as follows: a protein aqueous solution is mixed with a silver nitrate solution, and under alkaline conditions, it is reacted with a hydrogen peroxide solution to obtain the protein-Ag2O2 composite nanoparticles. The core-shell microneedles prepared by this invention, through hierarchical loading of silver peroxide nanoparticles and growth factors, respond to the microenvironment of infected skin wounds and perform programmed release, effectively preventing bacterial infection, promoting cell proliferation and angiogenesis, and driving tissue repair, effectively solving the problem of stagnant healing of infected wounds.

This invention specifically relates to a composite polymer antibacterial agent, its preparation method, and its application, belonging to the field of antibacterial materials technology. The antibacterial agent, by weight percentage, comprises a core component PQ-1 (0.01%~0.1%), a synergistic component polyhexamethylene biguanide (PHMB) (0.005%~0.05%), a synergistic component polyaminopropyl biguanide (PAPB) (0.005%~0.05%), and the balance being solvent. This invention, through the synergistic combination of PQ-1 and the two synergistic components, can reduce the minimum inhibitory concentration (MIC) against typical microorganisms by more than 75% while reducing the amount of PQ-1 by 50%~80%, achieving optimized protection of the antibacterial efficacy concentration of PQ-1, while also possessing the advantages of broad spectrum, stability, mildness, and low toxicity. Its preparation process is simple and can be widely applied in ophthalmic preparations, cosmetics, and other fields, demonstrating extremely high practical value.

The present invention relates to a novel indanone derivative, pharmaceutically acceptable salts or optical isomers thereof, a preparation method for same, and a pharmaceutical composition containing same as an active ingredient for preventing or treating viral diseases. The indanone derivatives according to the present invention not only have low cyto-toxicity, but can also demonstrate utility as pharmaceutical compositions for preventing or treating viral diseases such as:; polio, viral paralysis, acute hemorrhagic conjunctivitis, viral meningitis, hand-foot-mouth disease, blistering diseases, hepatitis A, myositis, myocarditis, pancreatitis, viral diabetes, epidemic myalgia, encephalitis, flu, herpes angina, foot- and-mouth disease, asthma, chronic obstructive pulmonary disease, pneumonia, sinusitis, otitis media, and the like, since excellent antiviral activity against picornaviruses such as Coxsackie virus, enterovirus, echovirus, polio virus, rhinovirus, and the like, have been demonstrated.

This invention discloses an intracellular imaging system for simultaneously detecting UDG and miR-375 expression levels and its applications. The intracellular imaging system uses a tetrahedral DNA nanostructure as a carrier, loading a HAT-UDG probe with an on / off conformation, and coupling it with a split CRISPR / Cas12a detection component for recognizing miR-375. The split CRISPR / Cas12a detection component includes at least Cas12a protein, scaffold RNA, spacer RNA / equivalent split crRNA components, and a fluorescent reporter substrate. This invention is the first to perform a logical AND operation between two key indicators: UDG enzyme activity and miR-375 expression level. The system can only ultimately trigger a complete fluorescence signal when both are simultaneously highly expressed. This multi-target synergistic activation design constructs a precise molecular recognition logic gate, enabling extremely accurate differentiation between prostate cancer cells (RV-1) and normal cells (293T), as well as other cancer cells (5637) that highly express UDG, fundamentally avoiding misjudgments caused by fluctuations in a single indicator or non-specific expression, resulting in extremely high imaging specificity.

This invention provides a 2,2'-binaphthyl-6,6'-dicarboxylic acid amino acid derivative, the structural formula of which is shown below: wherein the RNH is selected from one of the following. The 2,2'-binaphthyl-6,6'-dicarboxylic acid amino acid derivative exhibits antitumor activity.

This invention relates to the field of cosmetic technology, specifically to a salicylic acid supramolecular complex, its preparation method, and its applications. The components include salicylic acid, panthenol, and cyclodextrin; the weight ratio of salicylic acid to panthenol is (20-79):(40-59). The salicylic acid supramolecular complex prepared by this invention improves upon the inherent defect of salicylic acid's poor water solubility due to its lipid solubility. Furthermore, the prepared salicylic acid supramolecular complex maintains good solubility in various complex formulation systems containing commonly used cosmetic solvents such as ethanol and polyols.

This invention discloses a polyethylene composite fiber, its preparation method, and its applications. The polyethylene composite fiber comprises ultra-high molecular weight polyethylene fiber and bioceramics, and can be used in surgical suture and repair products. This surgical suture and repair product is mainly used for bone suture and repair, promoting bone growth and repair at the wound site, which is beneficial for bone healing. This surgical suture and repair product has high strength, is safe and reliable, has good biocompatibility, promotes bone wound healing, and is not easily adhered to bone tissue, making it easy to remove.

The application belongs to the technical field of biological control of agriculture, and particularly relates to a DNA pesticide as well as a preparation method and application thereof. The application provides a DNA pesticide, which comprises a nano-carrier and a genome of a fungal virus; the fungal virus is a plant pathogenic fungal virus, and can cause pathogenicity decline of a host fungus. The application uses the nano-carrier to load the genomic DNA of the fungal virus, so as to deliver the genomic DNA of the fungal virus to a plant pathogenic fungus, make the genomic DNA of the fungal virus replicate, transcribe and translate in the plant pathogenic fungus, and form a mature virus, thereby causing pathogenicity decline of the plant pathogenic fungus, and achieving the prevention and treatment of fungal diseases.

This invention relates to the field of antimicrobial peptide technology, and particularly to the antimicrobial peptide Ple-AB, its preparation method, and its applications. Using the fungal defensin Plectasin as a template, this invention designs and modifies the antimicrobial peptide Ple-AB. Compared with Plectasin, this antimicrobial peptide exhibits significantly higher antimicrobial activity and better bactericidal effects against Gram-positive bacteria; it also shows higher stability, particularly excellent resistance to trypsin; and its fermentation expression level is significantly improved. Furthermore, this antimicrobial peptide has very low hemolytic activity and cytotoxicity, making it suitable for development in antimicrobial drugs, additives, cosmetics, health products, and other fields, with broad application value and market prospects.

The application discloses an anti-thrombotic drug derivative carbon dot and a preparation method and application thereof. The method comprises the following steps: selecting an anti-thrombotic drug as a precursor, wherein the anti-thrombotic drug comprises at least one of an anti-platelet drug with a drug efficacy group, an anti-coagulation drug with a drug efficacy group and a fibrinolytic drug with a drug efficacy group, the molecular weight of the anti-thrombotic drug is 100-2000 Da, the thermal stability decomposition temperature is greater than or equal to 200 DEG C, and the drug efficacy group comprises at least one of a sulfonic acid group, an acetoxy group, an indole ring and a coumarin structure; performing gradient carbonization treatment on the precursor in multiple temperature zones, wherein the multiple temperature zones comprise a low-temperature zone capable of retaining the drug efficacy group, a medium-temperature zone capable of regulating the proportion of carbon hybridization and a high-temperature zone capable of performing heteroatom doping; and performing purification and drying treatment to obtain the anti-thrombotic drug derivative carbon dot. The anti-thrombotic drug derivative carbon dot obtained by the preparation method has both thrombolytic function and high safety, and can relieve the problem of imbalance between the curative effect and safety of the existing anti-thrombotic drug.

The present application relates to the technical field of medicine, and particularly discloses application of chiral hydroxychloroquine or a pharmaceutically acceptable salt thereof in preparation of a drug for preventing and treating immune diseases. The chiral hydroxychloroquine or the pharmaceutically acceptable salt thereof has a better inhibition rate and a lower cell survival rate on pathological cell proliferation caused by immune diseases, including but not limited to fibroblast-like synoviocytes; the chiral hydroxychloroquine or the salt thereof combined with methotrexate / ilaris (including but not limited to) can reduce the toxic side effects on normal cells and the cell survival rate, and has a better treatment effect on immune diseases.

This invention discloses a method for synthesizing bio-nano selenium using Beauveria bassiana, belonging to the field of nano-selenium synthesis technology. The method specifically includes the following steps: (1) activating and culturing Beauveria bassiana with accession number CGMCC 3.6914 in a liquid culture medium to obtain a spore suspension; (2) inoculating the spore suspension into a solid culture medium, simultaneously adding Na2SeO3 mother liquor, and then performing shaking culture. After the culture is completed, mixture a is obtained; (3) mixture a is separated, purified, and freeze-dried to obtain bio-nano selenium powder. The method for biosynthesizing nano-selenium in this invention utilizes Beauveria bassiana B26 (… Beauveria bassiana The metabolic activity of the substance reduces Na2SeO3 to nano-selenium, which has high synthesis efficiency, low cost and low pollution. The preparation process is simple and has great economic and environmental benefits.