64results about How to "Low cytotoxicity" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The present application provides a kind of polypeptide, wherein the polypeptide has the amino acid sequence as shown in SEQ ID NO.1 or the amino acid sequence as shown in SEQ ID NO.2;The inventors of the present application prepare a kind of high activity peptide mixture by the process of pearl powder enzymolysis, ultrafiltration, freeze-drying etc., the high activity peptide mixture has the efficacy of whitening, anti-aging etc.On this basis, the present application obtains two polypeptides SEQ ID NO.1, SEQ ID NO.2, the amino acid sequence of the SEQ ID NO.1 is TKLDHAVT, the amino acid sequence of the SEQ ID NO.2 is VPGF;In another embodiment of the present application, the inventors of the present application obtain polypeptide SEQ ID NO.1, SEQ ID NO.2 by self-synthesis method.In some specific embodiments of the present application, the inventors of the present application find that the aforementioned two polypeptides SEQ ID NO.1, SEQ ID NO.2 have significant melanin inhibitory activity.

The present invention provides a charge-reversible phospholipid that is not positively charged at a pH of the body fluid (typically in the neutral range) and has low cytotoxicity. The present invention provides a phospholipid represented by formula (1): wherein m represents a natural number of 9 to 25, n represents a natural number of 10 to 15, X1, X2, and X3 are the same or different and each represent H or OH, and R1 represents the following formula (i) or (ii): wherein p represents 1 or 2, q represents 1 or 2, and r represents an integer of 1 to 4; or wherein s represents an integer of 1 to 3, and R2 represents a hydrogen atom or a hydrocarbon group.

The invention relates to the technical field of polymer materials, and particularly provides a biocompatible polymer material as well as a preparation method and application thereof. The molecular structure of the biocompatible polymer material contains at least one first block and at least one second block, the first blocks and the second blocks are arranged alternately, and the adjacent first blocks and second blocks are connected through carbamate bonds; the first block is formed by aliphatic polyester diol and / or alicyclic polyester diol, and the number-average molecular weight of the first block is 1000-10000; the second block is formed by aliphatic polyether glycol, and the number-average molecular weight of the second block is 200-2000. The polyurethane material disclosed by the invention has the characteristic of relatively high mechanical strength, and the cytotoxicity of the polyurethane material is relatively low after the low-molecular-weight monohydric alcohol is further adopted for blocking. The polyurethane material can be used for biological tissue engineering or wound dressing.

The application discloses a drainage bag with an antibacterial functional layer and a preparation process thereof, and relates to the technical field of medical materials, and comprises the following steps: adding modified montmorillonite into N, N'-dimethylacetamide, uniformly mixing through ultrasonic, adding a polyurethane film, ultrasonic immersion for 60-120 min, drying, and obtaining a drainage bag substrate; adding polylactic acid into N, N-dimethylformamide, uniformly stirring and mixing, adding modified nano zinc oxide, uniformly mixing through ultrasonic, and then performing electrospinning to obtain an antibacterial coating fiber film; placing the antibacterial coating fiber film on the inner side surface of the drainage bag substrate, high-frequency heat sealing, assembling, and obtaining the drainage bag.

The invention belongs to the technical field of food fresh-keeping materials, and particularly relates to an aerogel type composite fresh-keeping pad as well as a preparation method and application thereof. The preparation method of the aerogel type composite fresh-keeping pad comprises the following steps: S1, mixing polyethylene glycol, oxalic acid and polyacrylic acid, and stirring at 70-80 DEG C to obtain a eutectic solvent; the method comprises the following steps: mixing a biomass raw material with a deep eutectic solvent, extracting, and filtering to obtain solid residues; s2, mixing the obtained solid residues with water, performing high-pressure homogenization treatment, mixing the obtained cellulose-containing suspension with a gelatin solution and a cross-linking agent, pouring the obtained mixed solution into a forming mold, performing freeze-drying treatment, and taking out to obtain the aerogel type composite fresh-keeping pad. The aerogel type composite fresh-keeping pad prepared by the method disclosed by the invention has good mechanical property, water absorption and antibacterial activity, and shows an excellent fresh-keeping effect in fresh-keeping of fish slices.

The application discloses an intrauterine postoperative anti-adhesion membrane, a preparation method thereof and a delivery device. The anti-adhesion membrane is a two-phase system composed of a natural polymer hydrogel outer layer and an inner layer of a specific block copolymer physically blended, forming a two-phase structure with gradient hydration function. The inner layer polymer is the core, covering ABA, AB or BAB type block structure, wherein the hydrophilic segment B can be selected from PEG, PEO, PNVP or PAA, and the hydrophobic segment A can be selected from PLA, PDLA, PGA, PCL, PTMC or a copolymer thereof. The total thickness of the material is 100-1000 microns, which can swell in body fluid and completely degrade within 7-30 days. The morphology is specially designed to accurately match the anatomical section of the target tissue. The application effectively isolates the wound surface and is mainly suitable for postoperative tissue adhesion prevention, uterine cavity repair, hemostasis and local drug release.

This invention discloses a weak force sensor based on a teardrop-shaped micro / nano optical fiber and its fabrication method. The sensor comprises a biconical micro / nano optical fiber, a single-conical optical fiber, a quartz capillary tube, and a PDMS microsphere. The biconical micro / nano optical fiber is symmetrically bent, with the central waist region fiber forming a teardrop-shaped ring. The two ends of the teardrop-shaped micro / nano optical fiber ring are sequentially connected to portions of the waist region fiber, a transition region fiber, and a standard fiber. This invention utilizes the sensitive self-sensing capability of micro / nano optical fibers to achieve integrated signal input and output, greatly simplifying the measurement feedback mechanism of the sensing system and reducing overall cost. Furthermore, it is simple and intuitive to operate, reducing the technical requirements for operators. In terms of sensor fabrication, the main raw materials are standard optical fiber and quartz capillary tube, both of which are extremely low-cost. Therefore, this micro / nano optical fiber sensor exhibits significant cost-effectiveness.

The invention belongs to the technical field of medicines, and particularly relates to a dihydropteridine-6 (5H)-ketone skeleton compound as well as a preparation method and application thereof. The dihydropteridine-6 (5H)-ketone skeleton compound disclosed by the invention is an HIV-1 (Human Immunodeficiency Virus-1) non-nucleoside reverse transcriptase inhibitor; the invention also comprises pharmaceutical salts, hydrates, solvates, polycrystals or co-crystals of the compounds, precursors and derivatives with the same biological functions of the compounds, a preparation method of the compounds and application of a composition containing one or more of the compounds in preparation of related drugs for treating AIDS and the like. The result of an in-vitro cellular level anti-HIV-1 activity experiment shows that the micromolecule has relatively strong anti-HIV-1 biological activity, can remarkably inhibit virus replication in MT-4 cells infected by HIV-1 viruses, and has relatively low cytotoxicity.

The invention relates to the technical field of biomedical materials, in particular to a nano assembly for releasing NO through red light triggering and a preparation method and application of the nano assembly. The nano assembly is prepared from an amphiphilic block polymer with a polyethylene glycol hydrophilic end and a hydrophobic segment containing NO donor molecules and photocatalyst molecules through a hydrophilic and hydrophobic self-assembly forming method. Photocatalyst molecules in the amphiphilic block polymer are excited under irradiation of red light, decomposition of NO donor molecules can be triggered through an electron transfer process, and space-time controllable adjustment of NO release is achieved. The nano assembly has the red light response characteristic, excellent biological safety and antibacterial property, and has a wide application prospect in the aspects of antibiosis, anti-inflammation and the like. In addition, the red light has the characteristics of large tissue penetration depth and low phototoxicity, so that the problem that the penetration depth of the traditional ultraviolet light or blue light response NO donor molecular monomer is limited is effectively solved.

This invention relates to the field of in vitro stem cell culture technology, specifically to a culture medium for the proliferation of muscle satellite cells, its culture method, and its application. The culture medium comprises a basal medium, fetal bovine serum, and plant-derived active substances; the plant-derived active substances include at least one selected from apigenin, resveratrol, and quercetin; the volume percentage of fetal bovine serum in the culture medium is 2%-5%. This invention, by adding plant-derived active substances, effectively maintains cell stemness and significantly promotes cell proliferation at low serum concentrations.

The application discloses an antibacterial peptide derived from the skin of bullfrog and application thereof, and the amino acid sequence of the antibacterial peptide is GSVGPVGPR (as shown in SEQ ID NO:1), and the molecular weight is 824.4504 g / mol. The antibacterial peptide has obvious inhibiting effect on Staphylococcus aureus, and can be used for preparing a medicine for preventing or inhibiting Staphylococcus aureus infection. Meanwhile, the antibacterial peptide and vitamin A have a significant synergistic bactericidal effect on Staphylococcus aureus. The antibacterial peptide and vitamin A can significantly reduce the use concentration of single component, improve the antibacterial efficiency, overcome the bacterial drug resistance problem, and can be used for preparing antibacterial medicines, skin external preparation, medical dressings, feed additives and food preservatives.

The application discloses an antibacterial peptide designed by a generative artificial intelligence framework and targeting gram-negative bacteria and an application thereof, and belongs to the fields of artificial intelligence and biotechnology. The application designs and successfully obtains a novel antibacterial peptide by a generative artificial intelligence framework, the antibacterial peptide targeting gram-negative bacteria and having good antibacterial activity. It is verified by experiments that the antibacterial peptide MTAMP003 has good bacteriostatic capacity on E. coli, S. typhimurium, P. aeruginosa and A. baumannii, exhibits low cytotoxicity on human gastric mucosa epithelium and low hemolyticity on rabbit red blood cells, and has good application prospect and research value.

Disclosed is an antigen targeting, anti-CD16A, and immune effector cell activating trifunctional fusion protein, comprising: a CD16A binding region that specifically binds to CD16A, a tumor-associated antigen (TAA) binding region that specifically binds to a TAA, an IL-15 / IL-15Rα complex formed by combining IL-15 and IL-15Ra, and an Fc domain. A designed tumor-targeting innate immune cell activation molecule, i.e., an anti-TAA, anti-CD 16A, and cytokine IL 15 trifunctional fusion protein targets NK cells to a tumor by simultaneously binding to a TAA on tumor cells and CD16A on NK cells, so that the NK cells release perforin and granzymes to induce apoptosis and cause tumor cell death. The cytokine IL15 expands and maintains the function of the NK cells, stimulates immune cell proliferation, and changes the immune microenviroment of the tumor.

This invention discloses a method for preparing a coumarin derivative and its application. The coumarin derivative is obtained by Perkin condensation and cyclization reaction of 2,4-dihydroxybenzaldehyde with ethyl acetoacetate to obtain compound (I). Compound (I) then undergoes a nucleophilic substitution reaction with 2,4-dinitrobenzenesulfonyl chloride to obtain the coumarin derivative, the chemical structure of which is shown in formula (II). The coumarin derivative (II) of this invention exhibits significant fluorescence responsiveness and high sensitivity to GSH in 0.2% DMSO-water solution, with a detection limit of 0.49 μM. Furthermore, this compound has low toxicity to H9c2 cells and can be used for the detection of GSH in live cells. This invention provides a simple and highly sensitive coumarin derivative for the detection of GSH, which has broad application prospects.

The application provides a fluorescent probe compound for simultaneously detecting HClO and SO2 at double sites and synthesis and application thereof. The probe compound takes phenothiazine as a response group of HClO, expands the conjugated structure of phenothiazine, and introduces a response group of SO2 and an organelle positioning group on the near-infrared fluorescent skeleton, thereby constructing an endoplasmic reticulum-targeted fluorescent probe BQTPA for simultaneously detecting HClO and SO2. The probe can realize dual-channel simultaneous detection of HClO and SO2 through two completely separated emission channels (λem=598 nm and 448 nm), and exhibits excellent selectivity, high sensitivity (detection limits are 7.9 nM and 33.8 nM, respectively) and good light stability to the response of HClO and SO2. The probe is successfully used for monitoring the changes of exogenous and endogenous HClO and SO2 levels in cells, further reveals the dynamic changes of the two in the process of drug-induced liver injury and ferroptosis, and clarifies the role of ferroptosis in liver injury.

The invention provides a BRCA specific siRNA which is used for enhancing the sensitivity of cancer cells to a PARP inhibitor. According to the present invention, the siRNA can be combined with the PARP inhibitor to induce cancer cell death, and is suitable for patients having the wild-type BRCA gene and having the low treatment effect on the PARP inhibitor. According to the present invention, the fusion protein-siRNA complex for delivering the siRNA can be absorbed by cells through CD47 mediation, such that the fusion protein-siRNA complex can be specifically delivered to cancer cells, and can be efficiently absorbed by the cells so as to maximize the desired cell death effect.

The invention discloses a near-infrared double-state light-emitting platinum complex fluorescent probe and application thereof in hypochlorite detection. The structural formula of the near-infrared double-state light-emitting platinum complex fluorescent probe is shown in the specification. R1 and R2 are one of H, CH3, C2H5 and C (CH3) 3; the four technical elements of near-infrared emission, double-state luminescence, a platinum complex phosphorescent probe and hypochlorite specific recognition are integrated into the same molecular system for the first time, and a novel platinum complex fluorescent probe which keeps efficient near-infrared luminescence in a solution state and an aggregation state and realizes high-sensitivity and high-selectivity detection on hypochlorite is provided. The double dilemma of solid state quenching of a traditional ACQ material and weak luminescence of a solution of a classical AIE material are broken through, and the blank in the technical field is filled. The novel platinum complex fluorescent probe shows high selectivity, high stability, wide pH application range, low cytotoxicity and good biocompatibility on hypochlorite.

This invention discloses an antimicrobial peptide, Cih1, and its applications. The amino acid sequence of antimicrobial peptide Cih1 is shown in SEQ ID NO.1. The antimicrobial peptide Cih1 provided by this invention is a cationic peptide, rich in cysteine ​​(-cys) residues, which can form an amphiphilic β-sheet secondary structure. This structure endows it with excellent antibacterial and antiviral activity, and can effectively inhibit the proliferation and invasion of pathogens in aquaculture water. Based on the broad-spectrum resistance of antimicrobial peptide Cih1 to pathogens, it can help aquatic animal hosts resist infection by various pathogens, and has broad application prospects in the field of anti-infective drug development for aquaculture.

The present application relates to the technical fields of genetic engineering and biological agents, and particularly relates to a hybrid peptide with immunoregulation and antioxidant functions, and a preparation method and application thereof.The polypeptide provided by the present application has both immunoregulation and antioxidant functions, and can significantly improve the immune function of the body and reduce the damage caused by immunosuppression to the body in normal or immunosuppressed states; and can enhance the antioxidant capacity of cells and the body; meanwhile, the polypeptide has the advantages of low cytotoxicity, high safety, simple preparation, low cost, and can be used as an ideal immunoregulator and antioxidant, and can be widely used in the fields of medicine, food, health care, feed, nutrition and the like of humans and animals, and has good application potential and value.

This application belongs to the field of biomedical materials and tissue engineering technology, and discloses an antibacterial coating based on MDI-cured resin osteogenic scaffold and its preparation method. It innovatively uses diphenylmethane diisocyanate (MDI) as a curing crosslinking agent. With the help of its excellent reactivity and chemical properties, a variety of antibacterial agents (including tannic acid, ornidazole and minocycline hydrochloride) are firmly bound to the surface of a 3D printed blank resin scaffold, and a coating with hydrophilicity, high efficiency antibacterial properties and low cytotoxicity is successfully constructed, which has good antibacterial effect against both Gram-positive and Gram-negative bacteria.

The present application relates to the technical field of plant active ingredient extraction, and particularly relates to a water-soluble rose extract and a preparation method and application thereof. Fresh rose flowers are frozen and crushed to obtain rose fragments; the rose fragments are subjected to supercritical carbon dioxide extraction to obtain a water-soluble rose extract; the supercritical carbon dioxide extraction conditions include an extraction temperature of 70-80 DEG C, an extraction time of 1-5 h, an extraction pressure of 10-15 MPa, and a separation pressure of 6-10 Mpa. In the present application, a rose extract is obtained through freeze-drying and optimization of supercritical carbon dioxide extraction conditions; the extract is a water-soluble transparent liquid with a fragrance, and has good compatibility. Meanwhile, the total flavonoid content of the rose extract is 80-110 mg RT / g DE, and the total polyphenol content is 140-210 mg GAE / g DE; subsequent experiments prove that the rose extract has low cytotoxicity, high stability, and good anti-inflammatory and antioxidant effects.

The application discloses a kind of compounds or its pharmaceutically acceptable salt, crystal form, solvate shown in formula I;Wherein, R1, R2, R3, R4 It is independently selected from halogen, C1~C4 Alkyl or halogen-substituted C1~C4 Alkyl.This kind of compound, it is connected by amido group with para-substituted phenyl and 3,5 position substituted benzyl to thiophene, novel and unique structure, to EV71, EVD68 Such as multiple enterovirus has good inhibitory activity and smaller cytotoxicity, provides a new potential choice for enterovirus infection with pharmacy drug.In addition, the application also discloses the preparation method and application of the compound shown in formula I, and its pharmaceutical composition.

The present application relates to a nucleic acid molecule for inducing asymmetric RNAi for inhibiting ROR-β (RAR related orphan receptor B) expression and use thereof, in particular, to a nucleic acid molecule for inducing asymmetric RNAi comprising an antisense strand comprising a sequence complementary to an mRNA encoding ROR-β and a sense strand forming a complementary bond with the antisense strand, and a pharmaceutical composition for improving or treating a retinal disease, the composition comprising the nucleic acid molecule for inducing asymmetric RNAi.

The present disclosure belongs to the field of medicinal chemistry, and relates to a novel pyridone derivative represented by Formula (I) or a stereoisomer, a pharmaceutically acceptable salt, a solvate or a crystal thereof, and use thereof in the preparation of a drug for preventing or treating diseases such as influenza type A viral infection and / or influenza type B viral infection, particularly use thereof as a PA subunit cap-dependent endonuclease inhibitor for preventing or treating diseases such as influenza type A viral infection and / or influenza type B viral infection. The compounds of the present disclosure have significant activity in inhibiting influenza endonuclease and influenza DNA, can be used either alone or in combination with a neuraminidase inhibitor, a nucleoside drug, a PB2 inhibitor, a PB1 inhibitor, an M2 inhibitor or other anti-influenza drugs, significantly shorten the time of influenza infection and reduces mortality, and have excellent clinical application prospects.

This invention discloses a core-shell microneedle loaded with silver nanoparticles and growth factors, its preparation method, and its application, belonging to the field of biomedical technology. The core-shell microneedle includes a backing layer and an array of needle tips disposed on the backing layer; the needle tips in the needle tip array have a core-shell structure, with the shell layer of the needle tip loaded with protein-Ag2O2 composite nanoparticles and the core layer of the needle tip loaded with growth factors. The preparation method of the protein-Ag2O2 composite nanoparticles is as follows: a protein aqueous solution is mixed with a silver nitrate solution, and under alkaline conditions, it is reacted with a hydrogen peroxide solution to obtain the protein-Ag2O2 composite nanoparticles. The core-shell microneedles prepared by this invention, through hierarchical loading of silver peroxide nanoparticles and growth factors, respond to the microenvironment of infected skin wounds and perform programmed release, effectively preventing bacterial infection, promoting cell proliferation and angiogenesis, and driving tissue repair, effectively solving the problem of stagnant healing of infected wounds.

This invention specifically relates to a composite polymer antibacterial agent, its preparation method, and its application, belonging to the field of antibacterial materials technology. The antibacterial agent, by weight percentage, comprises a core component PQ-1 (0.01%~0.1%), a synergistic component polyhexamethylene biguanide (PHMB) (0.005%~0.05%), a synergistic component polyaminopropyl biguanide (PAPB) (0.005%~0.05%), and the balance being solvent. This invention, through the synergistic combination of PQ-1 and the two synergistic components, can reduce the minimum inhibitory concentration (MIC) against typical microorganisms by more than 75% while reducing the amount of PQ-1 by 50%~80%, achieving optimized protection of the antibacterial efficacy concentration of PQ-1, while also possessing the advantages of broad spectrum, stability, mildness, and low toxicity. Its preparation process is simple and can be widely applied in ophthalmic preparations, cosmetics, and other fields, demonstrating extremely high practical value.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a DFO@GMs-pDA / PN composite scaffold for promoting vascularization and osteogenesis, and a preparation method and application thereof. The DFO@GMs-pDA / PN composite scaffold for promoting vascularization and osteogenesis is constructed by using electrospinning, pDA modification and emulsification-extraction technology, and by wrapping DFO into GMs and loading the DFO into a pDA surface modified PN electrospinning film. The most superficial DFO@GMs begin to disintegrate and release DFO after implantation, and the maximum drug release rate is reached after 48 hours, so as to promote the ingrowth of new blood vessels, the recruitment of stem cells and the secretion and presentation of related factors in the early stage of bone regeneration, and to provide an important blood supply basis for bone regeneration.

The present invention relates to a novel indanone derivative, pharmaceutically acceptable salts or optical isomers thereof, a preparation method for same, and a pharmaceutical composition containing same as an active ingredient for preventing or treating viral diseases. The indanone derivatives according to the present invention not only have low cyto-toxicity, but can also demonstrate utility as pharmaceutical compositions for preventing or treating viral diseases such as:; polio, viral paralysis, acute hemorrhagic conjunctivitis, viral meningitis, hand-foot-mouth disease, blistering diseases, hepatitis A, myositis, myocarditis, pancreatitis, viral diabetes, epidemic myalgia, encephalitis, flu, herpes angina, foot- and-mouth disease, asthma, chronic obstructive pulmonary disease, pneumonia, sinusitis, otitis media, and the like, since excellent antiviral activity against picornaviruses such as Coxsackie virus, enterovirus, echovirus, polio virus, rhinovirus, and the like, have been demonstrated.