Method for producing citric acid fermentation liquor by two-stage fermentation

A citric acid fermentation liquid and stage technology, applied in the field of microbial fermentation, to achieve the effects of shortening the seed culture time, shortening the fermentation cycle, and improving the conversion rate of sugar and acid

Inactive Publication Date: 2018-03-20
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the characterization of the activity of Aspergillus niger. How to improve the activity of Aspergillus niger through nutrient components (C/N) and regulate the two

Method used

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  • Method for producing citric acid fermentation liquor by two-stage fermentation
  • Method for producing citric acid fermentation liquor by two-stage fermentation
  • Method for producing citric acid fermentation liquor by two-stage fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] After crushing the corn, pass it through a 60-mesh sieve; mix the obtained corn flour with tap water at a mass ratio of 1:3, adjust the pH of the slurry to 6.0 with Ca(OH)2, and add 25 U / g of corn flour α—high-temperature amylase; the obtained slurry is first sprayed at 97°C, kept warm for 1.5 hours, then sprayed at 127°C, and the temperature of the feed liquid is reduced to 95°C after flash evaporation, and kept warm for 1 hour. After the iodine test is light brown, qualified corn liquefaction is obtained. liquid. The corn liquefaction solution is filtered through a plate frame to remove the filter residue to obtain the sugar solution.

[0029] Add 200L corn liquefaction solution and 100L tap water into the 500L seed tank as the seed medium. Take Aspergillus niger spores, add sterile water to make a spore suspension, transfer to a seed tank and cultivate for 16 hours to obtain a seed liquid.

[0030] In the first stage of fermentation, 400L of corn liquefaction liqui...

Embodiment 2

[0032] The preparation method of corn liquefaction liquid and sugar liquid is the same as that of Example 1, and the effects of seed cultivation and two-stage fermentation are mainly investigated.

[0033] Add 250L corn liquefaction solution and 150L tap water into the 500L seed tank as the seed medium. Take Aspergillus niger spores, add sterile water to make a spore suspension, transfer to a seed tank and cultivate for 24 hours to obtain a seed liquid.

[0034] In the first stage of fermentation, add 600L of corn liquefaction liquid into a 5000L fermenter, control the C / N at 15, lower the temperature to 39°C, transfer 400L of seed liquid to the fermenter for 2 hours; The liquid is first cooled by a plate heat exchanger outside the tank. After the temperature reaches 39°C, it is added to the fermenter, and the addition is completed within 12 hours. The volume of the sugar solution is 3000L. The C / N is controlled at 55, and the reducing sugar is lower than 5g / L. Stop Ferment t...

Embodiment 3

[0036] The preparation method of corn liquefaction liquid and sugar liquid is the same as that of Example 1, and the effects of seed cultivation and two-stage fermentation are mainly investigated.

[0037] Add 225L corn liquefaction solution and 125L tap water into the 500L seed tank as the seed medium. Take Aspergillus niger bran spores, add sterile water to make a spore suspension, transfer to a seed tank and cultivate for 20 hours to obtain a seed liquid.

[0038] In the first stage of fermentation, 500L of corn liquefaction liquid was added to a 5000L fermenter, and the C / N was controlled at 20. The liquid is first cooled by a plate heat exchanger outside the tank. After the temperature reaches 37°C, it is added to the fermentation tank, and the addition is completed within 6 hours. The volume of the sugar solution is 3100L. The C / N is controlled at 70, and the reducing sugar is lower than 5g / L. Stop Ferment to obtain citric acid fermentation liquid.

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Abstract

The invention discloses a method for producing citric acid fermentation liquor by two-stage fermentation. The method comprises the following steps: (1) Aspergillus niger mouldy bran spores are taken,sterile water is added in order to prepare a spore suspension, the suspension is inoculated to a seed tank and culture is carried out for 16-24 hours, in order to obtain seed liquid; (2) corn liquefaction liquid whose volume is 8-12% of effective volume of a fermentation cylinder is added into the fermentation cylinder, and the temperature is reduced to 35-39 DEG C; (3) seed liquid obtained in thestep (1) is inoculated to the fermentation cylinder, and cultivation is carried out for 2-8 hours; (4) a sugar solution whose volume is 60-64% of the effective volume is added into the fermentation cylinder for cultivation, and when reducing sugar is below 5g/L, fermentation is stopped, and citric acid fermentation liquor is obtained. The two-state cultivation avoids influences of pH reduction inearly stage of fermentation and concentration of nutrients on growth of fungi, improves utilization efficiency and utilization rate of starchiness raw materials, reduces residual sugar of fermentation, and improves conversion rate of saccharic acid; the method can improve acid production rate and shorten fermentation period.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for preparing citric acid fermentation liquid through two-stage fermentation. Background technique [0002] Citric acid is a typical biological fermentation product and is the most productive organic acid. Its main production method is microbial submerged fermentation. The industrial production of citric acid fermentation has been developed for many years and has basically formed a mature production process, but there are still many aspects of the production process that need to be improved. At present, the traditional citric acid fermentation process is: first cultivate Aspergillus niger spores into seed liquid, and then transfer the seed liquid to a fermenter for cultivation to obtain a fermented liquid. The production of citric acid mainly uses corn as a raw material. The corn is crushed and sprayed twice with enzyme to obtain corn liquefaction liquid...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
CPCC12P7/48
Inventor 石贵阳刘龙陈坚胡志杰蒋建伟蒋小东金赛孙福新李由然周东姣
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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