1214 results about "Spore" patented technology

Disclosed is a novel Streptomyces sporosporus strain SC263 produced by UV mutagenesis, the produced antibiotics exhibit increased thermal stability, which exhibit antibiotic activity against bacteria and fungi. Antibiotics produced by SC263 and compositions thereof are also provided for the treatment of bacterial and fungal infections. The antibiotics have a very high molecular weight, which is suitable for topical administration.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention relates to a coral sand soil multi-dimensional ecological low-carbon reinforcement method which comprises the following steps: S1, a urease solution is sprayed on a coral sand single-particle soil layer, a cementing liquid A is sprayed after standing, the urease solution is extracted from legume plants, and the cementing liquid A is obtained by mixing a urea solution and a calcium chloride solution; s2, on the basis of treatment in the step S1, a sporosarcina pasteurii bacterial solution is firstly sprayed, after standing, a cementing solution B is sprayed, the cementing solution B is alternately and circularly sprayed for multiple times, and the cementing solution B is obtained by mixing a urea solution and a calcium chloride solution; s3, a 3D printing geosynthetic material is laid on the basis treated in the step S2; and S4, laying a coral sand single-particle soil layer on the basis of treatment in the step S3, and repeating the steps S1 to S3. According to the method, a single particle size-foundation unit size-engineering size reinforcing system is constructed, comprehensive reinforcing from a microscopic single particle to a macrostructure can be achieved, and the reinforcing effect and efficiency are effectively improved.

The invention discloses a quintuple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit for simultaneously detecting five parasites as well as special primers and probes thereof, and belongs to the technical field of biological detection. The kit provided by the invention can be used for simultaneously detecting cryptosporidium parvum, strongyloid stercoides, trypanosoma kirschneri, theileria and echinococcus granulosus in the same system, and has the characteristics of qualitative and quantitative properties, high sensitivity, strong specificity, good repeatability and good stability.

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness white flammulina velutipes HJ120 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme of the invention, the high-brittleness white flammulina velutipes HJ120 is obtained, and the preservation number of the flammulina velutipes HJ120 is CCTCC (China Center for Type Culture Collection) NO: M 20242218. The invention also provides a food which comprises the white flammulina velutipes, spores of the white flammulina velutipes or / and an extract of the white flammulina velutipes.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses a salt-tolerant growth-promoting bacillus cereus strain and an application thereof. In particular discloses bacillus cereus T146, and the preservation number of the bacillus cereus T146 is CGMCC (China General Microbiological Culture Collection Center) No.30429. The bacillus cereus T146 disclosed by the invention has good biofilm production, siderophore secretion and nitrogen fixation capabilities, and can remarkably increase the root length, the overground dry weight, the plant height, the number of lateral roots and the average density of the lateral roots of wheat under the condition of salt stress. According to the invention, a low-cost, efficient and applicable culture medium aiming at the bacillus cereus T146 is determined, a stable and controllable fermentation system is established, the preparation method of the microbial inoculum with high mature spore number is obtained by optimizing a spray drying process, and the active spore number of the microbial inoculum can reach 1.3 * 10 < 11 > CFU / g. The bacillus cereus T146 has good development and application prospects in the fields of microbial agents, microbial fertilizers, soil improvement and the like.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, Ilyonectria fungus hyphae are prepared, Ilyonectria fungi are cultured on a PDA solid culture medium for 14 d, then the Ilyonectria fungi are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture for 4 d and filtered, filtrate is centrifuged for 10 min at the speed of 10000 rpm, and spores are collected; diluting the concentration of spores to 107 pieces / mL, inoculating the spores into a YEPD liquid culture medium, carrying out shake-flask culture for 2 days, and filtering and collecting hyphae; and S12, preparing the Ilyonectria fungus protoplast, namely adding the hypha prepared in the step S11 into the enzymatic hydrolysate, performing enzymolysis in a shaker at the temperature of 30 DEG C at the rotating speed of 150-200rpm for 3-5 hours, filtering, centrifuging filtrate at the temperature of 4 DEG C, discarding supernate, adding 0.7 M NaCl for centrifuging, and discarding the supernate, thereby obtaining the Ilyonectria fungus protoplast. The genetic transformation constructed by the invention can be used for exploring the specific action and mechanism of a specific pathogenic gene in the pathogenic process of Ilyonectria fungi through gene knockout or overexpression experiments.

A method of production of granular soil conditioner based on a mixture of biochar and organic fertilizer comprising the following steps:Biochar mixing with animal excrements in a ratio of 1 part of biochar to 0.1 to 0.4 parts of the excrement dry matter, adding to the base of a mixture of soil bacteria belonging to at least two genera of the following: bacteria of the Rhizobia genus, nitrifying bacteria of the Azospirillum or Azotobacter genus, bacteria of the Pseudomonas genus, and bacteria of the Bacillus genus,topping the bacteria-endowed base with water in the weight not more than double the weight of the bacteria-endowed base and letting the mixture ferment for at least 5 days,draining off the excess water and drying the bacterial base so that the water content does not exceed 20% by weight,adding a mixture of spores of fungi containing at least two of the group including Trichoderma, Arbuscular mycorrhizal fungi, Ectomycorrhizal fungi to the base-bacteria compound in a total quantity corresponding to 0.2 to 1% of the dry matter weight of the base-bacteria compound.

The invention relates to bacillus velezensis and application thereof in prevention and treatment of wheat leaf blight and inhibition of plant pathogenic fungi, and belongs to the field of biological prevention and treatment. The bacillus velezensis HXYT7 is separated and screened from tobacco leaves growing in a tobacco plantation (the longitude is 113.73 and the latitude is 33.91) in Xiangchang County in elm city, Henan province, has a remarkable inhibition effect on mophila, fusarium moniliforme, fusarium graminearum, rhizoctonia cerealis, aspergillus flavus and aspergillus niger, can damage the hypha structure of the mophila, and can be used for preparing the bacillus velezensis HXYT7. The bacillus subtilis can inhibit germination of spores of the helminthosporium, has an obvious prevention and treatment effect on the wheat leaf blight caused by the helminthosporium, and provides a new microbial resource for prevention and treatment of the wheat leaf blight.

The invention relates to the technical field of agricultural micro-ecological application, and particularly discloses a composite combined inoculation method using arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, which comprises the following steps: selecting corn plant seeds without plant diseases and insect pests; a corn planting land is randomly divided into land parcels with the area of 6 m < 2 > (2 m * 3 m), a buffer zone of 1.0 m is arranged between every two adjacent land parcels, and the cultivation mode is flat cropping; the method comprises the following steps: planting arbuscular mycorrhizal fungi inoculum comprising mycorrhiza, dry sand containing spores and ectotrophic hyphae and corn seeds in soil by using a seeder during corn sowing, and after the corn is planted for 30 days, inoculating the plant growth-promoting rhizobacteria inoculum into plants and soil by using a flood irrigation root irrigation method, when the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are inoculated together, the corn rhizosphere microbial community can be changed, the richness and diversity of fungi and bacterial communities in soil are increased, and the relative abundance of plant pathogens in the soil is reduced.

The present invention belongs to the technical field of microbial agents, and specifically relates to a strain of Emericellopsispallida F27, a microbial agent and its application in the field of agriculture. The present invention provides a strain of Emericellopsispallida F27, with a deposit number of CGMCC No. 40123, and a microbial agent is prepared using Emericellopsispallida F27. The application results show that the strain of Emericellopsispallida F27 can increase the degradation rate of corn stalks by 30.55%, and the reducing sugar concentration in the culture solution reaches 25.3 mg / L. Emericellopsispallida F27 can significantly increase the proportion of large aggregates in the soil and significantly inhibit the growth of various sickle fungi. The application of the Emericellopsispallida F27 microbial agent can promote the growth of corn and wheat, improve the stress resistance of corn, and increase soil microbial biomass carbon, microbial nitrogen, soil soluble nitrogen, soil oxygen content and straw degradation rate.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention relates to a spaceflight-carried schizophragma integrifolium strain Kungsu No.3 and application thereof, and belongs to the technical field of strain breeding. The spaceflight-carried schizophyllum commune strain No.3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 9, 2024, the preservation number is CGMCC No.41428, the classification name of the spaceflight-carried schizophyllum commune strain is schizophyllum commune, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with cultivation of strains which are not subjected to spaceflight carrying, the Kunzhou No.3 strain is fast in hypha germination, fast in growth, crisp in sporocarp, free of or few in spores, light pink to pink, high in conversion rate, short in period and suitable for industrial cultivation.

The invention relates to the technical field of biocontrol strains for crops, in particular to bacillus velezensis JR1A and application thereof to inhibition of garlic pathogenic fungi. The bacillus velezensis JR1A is preserved in the China General Microbiological Culture Collection Center on June 30, 2025, and the preservation number of the bacillus velezensis JR1A is CGMCC No.35059. The bacillus velezensis JR1A is named as bacillus velezensis JR1A. The JR1A inhibits mycelial growth and spore production of pathogenic fungi of common garlic diseases, has an excellent biocontrol effect and a certain growth promoting function, can reduce the relative abundance of pathogenic fungi in garlic crop rotation soil, and reduces the occurrence of related garlic diseases.

The invention relates to the technical field of soil remediation, and provides a soil pollution remediation process which comprises the following steps: distributing points in grids of an area to be remediated, measuring the content of heavy metals and the concentration of organic pollutants, applying multi-frequency microwave radiation, synchronously applying ultrasonic treatment, and injecting a complex microbial inoculant containing sphingomonas, aspergillus niger spores and a nitrogen and phosphorus nutrient solution. A direct-current electric field is applied, pH, oxidation-reduction potential Eh and temperature parameters are monitored and measured in real time through an embedded sensor, a repair by-product recovery system is arranged, the purity of recovered metal is not lower than 98%, and mobile repair equipment is used in a matched mode. According to the method, the pollution degree is accurately quantified by constructing the pollution index PI, and dynamic regulation and repair are realized in combination with real-time monitoring parameters of a sensor. The pollutant removal efficiency is improved through the synergistic effect of multiple technologies, metal resource recovery is achieved through a repair by-product recovery system, traditional problems are solved, and efficient and environment-friendly repair is achieved.

The invention relates to a ganoderma lucidum strain L4905 and a cultivation method and application thereof, and belongs to the technical field of biology. The ganoderma lucidum strain L4905 is preserved in Guangdong Microbial Culture Collection Center on October 9, 2023, and the preservation number is GDMCC No: 63866. The ganoderma lucidum strain L4905 is short in growth cycle and stable in character. The strain has the characteristics of high gloss of sporocarp paint sample, kidney-shaped pileus, thick mushroom pulp, high yield of sporocarp and spore powder, stable cultivation character, high polysaccharide content and the like, and is suitable for substitute cultivation and popularization in most areas of Yunnan.

Detergent composition comprising bacterial spores, polyamine and enzyme.

The invention discloses a quintuple real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection kit and a detection method for simultaneously detecting five parasites, and belongs to the technical field of biological detection. The method and the kit provided by the invention can be used for simultaneously detecting toxoplasma gondii, neosporidium, Eimeria stigmas, Giardia blue and trypanosoma evanescens in the same system, and have the characteristics of qualitative and quantitative properties, high sensitivity, strong specificity, good repeatability and good stability, and can be used for simultaneously detecting the toxoplasma gondii, the neosporidium, the Eimeria stigmas, the Giardia blue and the trypanosoma evanescens.

The invention belongs to the field of microalgae-fungus bioengineering and the technical field of lignin wastewater treatment, and discloses a method for synthesizing grease by utilizing lignin wastewater through microalgae-fungus co-culture, which comprises the following steps: (1) inoculating microalgae into a first culture medium to obtain a microalgae seed solution; the microalgae is chlorella; inoculating the fungal spores into a second culture medium, and carrying out shake cultivation to obtain fungal pellets, so as to obtain a fungal seed solution; the fungi are cunningham echinophora; preparing lignin wastewater, adding culture medium inorganic salt into the lignin wastewater, and sterilizing to obtain a lignin wastewater culture medium; and (2) co-inoculating the fungus seed solution and the microalgae seed solution into a lignin wastewater culture medium for co-culture to obtain the microalgae-fungus symbiont synthesized with grease. According to the method, the lignin wastewater is treated through co-culture of microalgae and fungi, removal of organic pollutants in the lignin wastewater and enrichment of lignin can be synchronously and effectively achieved, and the yield of grease is increased.

A laundry liquid composition comprising a surfactant, a polyamine and bacterial spores and a method for treating a textile.

The invention is applicable to the technical field of silkworm cocoon quality detection, and provides a silkworm cocoon surface pollution detection method, which is used for performing non-destructive detection on silkworm cocoon surface pollution by combining a high-sensitivity confocal Raman spectrum analysis system and a self-adaptive spectrum analysis technology based on a Raman spectrum analysis technology. According to the self-adaptive spectrum analysis technology, firstly, a probe on a scanning platform is used for curved surface reconstruction based on pre-scanning point cloud data based on an irradiation sampling surface of a sample; screening out pre-scanning points which do not contain microbial spores, and generating a three-dimensional data model which is highly matched with the distribution of microorganisms on the silkworm cocoons; corresponding to the reconstructed three-dimensional coordinates of the remaining multiple points, analyzing the corresponding Raman spectrograms one by one by utilizing Raman detection to obtain an identification result. Therefore, the method can significantly reduce the operation complexity and accelerate the overall detection rate.

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The invention provides bacillus velezensis as well as a preparation method and application thereof. The bacillus velezensis and the microbial inoculum or composition containing the bacillus velezensis can effectively inhibit pathogenic microorganisms of plant root rot. The culture medium provided by the invention has the advantages of simple and easily available components, low price, simple and feasible fermentation conditions and short fermentation period. Moreover, the prepared fermentation liquor has higher viable count, spore count and spore conversion rate and lower effective viscosity, almost has no influence on subsequent treatment, and can be simply filtered, concentrated and then spray-dried. The prepared powder product is stable in character, and industrial production and popularization and application of bacillus related products are facilitated.

Agrochemical compositions including an agriculturally suitable carrier; one or more encysted or sporulated protozoa (protist); and an agricultural payload including an agrochemical with or without a food source for protozoa such as a agriculturally nonbeneficial bacteria are provided. Also provided is a modified plant seed, and methods for improving plant growth, reducing plant loss to pests, and controlling or eliminating pest infestation of a crop plant, each including use of the described agrochemical compositions.

The present invention provides a 5-hydroxytryptophan production strain and a construction method and application thereof. The strain has a clear genetic background and is effective in producing 5-hydroxytryptophan. E.coli HTP11 Subtilis subtilis spores pureF bsu K316Q Mutated gene, upregulated strain E.coli HTP11 of guaB , guaA , gmk , ndk The transcription level of the gene was increased by heterologous introduction of the gapX Gene, missing aromatic amino acid transporter serB , tnaB , aroP Gene, up-regulated inner membrane efflux transporter yhjV The transcription level of the gene was increased and electroporated into the pTm-serB plasmid, which could stably provide 5-hydroxytryptophan, the direct precursor of 5-hydroxytryptophan, and had a high efficiency of 5-hydroxytryptophan output.

The invention discloses a pseudo-ginseng bHLH transcription factor gene PnbHLH2, the nucleotide sequence of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is as shown in SEQ ID NO: 1, a bHLH transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnbHLH2 gene has the capability of improving fungal infection resistance of plants, the PnbHLH2 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to fusarium latericum, fusarium moniliforme, humicola fucoatra and cylindrocarpon destructor is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the humicola fucoatra and the cylindrocarpon destructor is obviously enhanced. The transgenic tobacco overexpressed with the PnbHLH2 has the advantages that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the fusarium moniliforme and the cylindrocarpon destructor is obviously improved;

The invention relates to the technical field of solenopsis invicta prevention and control, in particular to metarhizium palustris for preventing and controlling solenopsis invicta as well as engineering bacteria and application of the metarhizium palustris. The Metarhizium anisopliae provided by the invention is a strain which is separated from the field and has high toxicity to the solenopsis invicta, and can be used for preventing and treating the solenopsis invicta or constructing engineering bacteria for preventing and treating the solenopsis invicta. The engineering bacterium constructed by the invention has high toxicity to solenopsis invicta, and can enable the bombyx batryticatus infected with the solenopsis invicta to volatilize a large amount of a solenopsis invicta attractant longifolene, so that the bombyx batryticatus has a trapping and killing effect on the worker solenopsis invicta, and the sanitary behavior of the worker is obviously slowed down. When the engineering bacteria constructed by the invention are combined with a small amount of chemical insecticide bait, the control effect of wettable powder taking spores of the engineering bacteria as an active component on a multi-ant nest (a single-ant nest) of solenopsis invicta is 79% (58%) within 3 days, 93% (90%) within 7 days and 98% (98%) within 28 days, and the method has the advantages of safety, high efficiency and the like.

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.