732 results about "Spore" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention relates to a coral sand soil multi-dimensional ecological low-carbon reinforcement method which comprises the following steps: S1, a urease solution is sprayed on a coral sand single-particle soil layer, a cementing liquid A is sprayed after standing, the urease solution is extracted from legume plants, and the cementing liquid A is obtained by mixing a urea solution and a calcium chloride solution; s2, on the basis of treatment in the step S1, a sporosarcina pasteurii bacterial solution is firstly sprayed, after standing, a cementing solution B is sprayed, the cementing solution B is alternately and circularly sprayed for multiple times, and the cementing solution B is obtained by mixing a urea solution and a calcium chloride solution; s3, a 3D printing geosynthetic material is laid on the basis treated in the step S2; and S4, laying a coral sand single-particle soil layer on the basis of treatment in the step S3, and repeating the steps S1 to S3. According to the method, a single particle size-foundation unit size-engineering size reinforcing system is constructed, comprehensive reinforcing from a microscopic single particle to a macrostructure can be achieved, and the reinforcing effect and efficiency are effectively improved.

The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a high-brittleness white flammulina velutipes HJ120 strain and application thereof. The technical problem to be solved by the invention is to cultivate a flammulina velutipes variety with better quality. According to the technical scheme of the invention, the high-brittleness white flammulina velutipes HJ120 is obtained, and the preservation number of the flammulina velutipes HJ120 is CCTCC (China Center for Type Culture Collection) NO: M 20242218. The invention also provides a food which comprises the white flammulina velutipes, spores of the white flammulina velutipes or / and an extract of the white flammulina velutipes.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention relates to the technical field of agricultural micro-ecological application, and particularly discloses a composite combined inoculation method using arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, which comprises the following steps: selecting corn plant seeds without plant diseases and insect pests; a corn planting land is randomly divided into land parcels with the area of 6 m < 2 > (2 m * 3 m), a buffer zone of 1.0 m is arranged between every two adjacent land parcels, and the cultivation mode is flat cropping; the method comprises the following steps: planting arbuscular mycorrhizal fungi inoculum comprising mycorrhiza, dry sand containing spores and ectotrophic hyphae and corn seeds in soil by using a seeder during corn sowing, and after the corn is planted for 30 days, inoculating the plant growth-promoting rhizobacteria inoculum into plants and soil by using a flood irrigation root irrigation method, when the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are inoculated together, the corn rhizosphere microbial community can be changed, the richness and diversity of fungi and bacterial communities in soil are increased, and the relative abundance of plant pathogens in the soil is reduced.

The invention discloses a bacillus coagulans feed additive and a preparation method thereof, and relates to the technical field of feed additives, the bacillus coagulans feed additive comprises bacillus coagulans spores, the content of the spores is 1.0 * 10 < 8 >-1.0 * 10 < 11 > CFU / g; the protective agent system comprises the following components: maltodextrin or lactose, vegetable protein, polyhydric alcohol and an antioxidant; the invention discloses a microcapsule coating structure. A microcapsule is a two-layer composite coating of an inner layer alginate matrix and an outer layer polycation or polymer coating. According to the invention, bacillus coagulans spores which can still keep high survival rate under short-time high-temperature and low-pH conditions are obtained through screening and fermentation strategies of strains with high sporulation and heat and acid tolerance, the problem that common strains are easy to inactivate in granulation, extrusion and gastric acid environments is solved, the problem of product failure caused by insufficient tolerance of the strains is reduced, and the product quality is improved. And the original biological activity basis is improved, so that the protection effect of the subsequent formula and the coating can be played at a higher starting point, and the zoology effect of the final preparation is improved.

The invention relates to the technical field of biocontrol strains for crops, in particular to bacillus velezensis JR1A and application thereof to inhibition of garlic pathogenic fungi. The bacillus velezensis JR1A is preserved in the China General Microbiological Culture Collection Center on June 30, 2025, and the preservation number of the bacillus velezensis JR1A is CGMCC No.35059. The bacillus velezensis JR1A is named as bacillus velezensis JR1A. The JR1A inhibits mycelial growth and spore production of pathogenic fungi of common garlic diseases, has an excellent biocontrol effect and a certain growth promoting function, can reduce the relative abundance of pathogenic fungi in garlic crop rotation soil, and reduces the occurrence of related garlic diseases.

The invention relates to the technical field of soil remediation, and provides a soil pollution remediation process which comprises the following steps: distributing points in grids of an area to be remediated, measuring the content of heavy metals and the concentration of organic pollutants, applying multi-frequency microwave radiation, synchronously applying ultrasonic treatment, and injecting a complex microbial inoculant containing sphingomonas, aspergillus niger spores and a nitrogen and phosphorus nutrient solution. A direct-current electric field is applied, pH, oxidation-reduction potential Eh and temperature parameters are monitored and measured in real time through an embedded sensor, a repair by-product recovery system is arranged, the purity of recovered metal is not lower than 98%, and mobile repair equipment is used in a matched mode. According to the method, the pollution degree is accurately quantified by constructing the pollution index PI, and dynamic regulation and repair are realized in combination with real-time monitoring parameters of a sensor. The pollutant removal efficiency is improved through the synergistic effect of multiple technologies, metal resource recovery is achieved through a repair by-product recovery system, traditional problems are solved, and efficient and environment-friendly repair is achieved.

The invention belongs to the field of microalgae-fungus bioengineering and the technical field of lignin wastewater treatment, and discloses a method for synthesizing grease by utilizing lignin wastewater through microalgae-fungus co-culture, which comprises the following steps: (1) inoculating microalgae into a first culture medium to obtain a microalgae seed solution; the microalgae is chlorella; inoculating the fungal spores into a second culture medium, and carrying out shake cultivation to obtain fungal pellets, so as to obtain a fungal seed solution; the fungi are cunningham echinophora; preparing lignin wastewater, adding culture medium inorganic salt into the lignin wastewater, and sterilizing to obtain a lignin wastewater culture medium; and (2) co-inoculating the fungus seed solution and the microalgae seed solution into a lignin wastewater culture medium for co-culture to obtain the microalgae-fungus symbiont synthesized with grease. According to the method, the lignin wastewater is treated through co-culture of microalgae and fungi, removal of organic pollutants in the lignin wastewater and enrichment of lignin can be synchronously and effectively achieved, and the yield of grease is increased.

The invention discloses a yellow flammulina velutipes ZJJZG002 and InDel marker with high resistance and high brittleness and application of the yellow flammulina velutipes ZJJZG002 and InDel marker, and relates to the technical field of edible mushroom cultivation, the preservation number of the yellow flammulina velutipes is CGMCC NO.41174, and the yellow flammulina velutipes is classified and named as flammulina velutipes. The strain ZJJZG002 takes yellow flammulina velutipes F24 and white flammulina velutipes W1 as parents, the parent materials are subjected to ultraviolet mutagenesis treatment and then are subjected to single spore isolation, hybridization (a hybrid combination XB51 * YU19) and fruiting tests, and the strain ZJJZG002 is not prone to umbrella opening, rich in mushroom fragrance, crisp and refreshing in taste, neat in fruiting, suitable for production in various cultivation modes and capable of injecting a new seed source for the domestic flammulina velutipes market.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The invention discloses a pseudo-ginseng bHLH transcription factor gene PnbHLH2, the nucleotide sequence of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is as shown in SEQ ID NO: 1, a bHLH transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnbHLH2 gene has the capability of improving fungal infection resistance of plants, the PnbHLH2 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the pseudo-ginseng bHLH transcription factor gene PnbHLH2 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to fusarium latericum, fusarium moniliforme, humicola fucoatra and cylindrocarpon destructor is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the humicola fucoatra and the cylindrocarpon destructor is obviously enhanced. The transgenic tobacco overexpressed with the PnbHLH2 has the advantages that the resistance of the transgenic tobacco overexpressed with the PnbHLH2 to the fusarium latericum, the fusarium moniliforme, the fusarium moniliforme and the cylindrocarpon destructor is obviously improved;

The invention discloses a biological challenge device with combined adjustable resistance for evaluating the effect of a sterilization process, which comprises a shell, the shell comprises a plastic pipe and an inner pipe cap detachably connected with the inner wall of the plastic pipe, the inner pipe cap is provided with a breathable window and covered by a breathable film, and a bacterial contamination carrier for bearing indicator biological spores is attached to the inner bottom of the plastic pipe. An openable container containing a liquid culture medium is arranged in the plastic pipe cavity; the outer side of the inner pipe cap is sleeved with an outer pipe cap detachably connected with the outer wall of the plastic pipe, and the outer pipe cap is provided with a flow blocking structure used for adjusting the speed of the sterilizing agent entering the area of the inner pipe cap; the indicator biological spores are pretreated by a stable protective agent containing one or more of polyethylene glycol, trehalose, sorbitol, polyvinylpyrrolidone and glyceryl stearate. The speed of a sterilizing agent entering the device can be limited, the spores are dry in a suspension added with a stable protective agent, the capability of resisting general environmental changes is very good, and the performance of the device can be maintained for a long time.

The invention relates to the technical field of environmental remediation, and particularly discloses a soil remediation agent for heavy metal pollution and a preparation method of the soil remediation agent. The soil remediation agent is prepared from 30 to 40 parts of modified biomass charcoal, 15 to 20 parts of chitosan-humic acid composite gel, 5 to 8 parts of arbuscular mycorrhizal fungal spore suspension, 3 to 5 parts of heavy metal-resistant microbial strains, 2 to 3 parts of plant-based nano zero-valent iron, 10 to 15 parts of sodium alginate solution, 1 to 2 parts of compound enzyme preparation and 8 to 12 parts of bio-based calcium carbonate. According to the invention, the adsorption material is prepared from the waste biomass, and a dynamic response remediation system is constructed by combining microbial microencapsulation and multi-component synergistic proportioning, so that efficient fixation of heavy metals, soil ecological protection and remediation cost optimization are realized, and the problems of high reversibility risk, large ecological disturbance, insufficient soil adaptability and the like of a traditional remediation agent are solved.

The invention discloses a cement-based permeable nanocrystal self-repairing waterproof admixture and a preparation method thereof, and belongs to the technical field of building materials. The admixture is composed of quick-hardening sulphoaluminate cement, nano silicon dioxide, nano anhydrous calcium carbonate, bacillus pasteurii and the like, nanoparticles are subjected to surface modification through a silane coupling agent, microorganisms are embedded through sodium alginate to form microcapsules, the mineral activity is improved in combination with ball milling treatment, and a chemical expansion and microorganism mineralization double-repair system is constructed. After the nano-powder is modified, the dispersity is improved by 60%, pores of 10-50 nm can be filled, the survival rate of spores can reach 85% or above through microbial embedding, activation is conducted within 48 hours when the nano-powder meets water, the self-repairing efficiency of a double-repairing system on cracks of 0.1-0.3 mm is larger than or equal to 90%, and the anti-permeability pressure recovery rate after repairing is larger than or equal to 80%. The 7d anti-permeability pressure of the product is greater than or equal to 1.2 MPa, and the 28d anti-permeability pressure of the product is greater than or equal to 1.6 MPa, and the product has early-stage rapid repairing and long-term waterproof performance, solves the problems of insufficient durability and low self-repairing efficiency of a traditional material, and is suitable for underground engineering, bridges, tunnels and other scenes.

The invention relates to the technical field of biology, in particular to pure bacillus simplex ZF535 capable of producing exopolysaccharides and application of the pure bacillus simplex ZF535. The preservation registration number of the simple pure bacillus ZF535 disclosed by the invention in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No: 34733. Pot experiment results show that leaf spraying or root irrigation treatment of exopolysaccharides of the strain ZF535 has a remarkable induced disease-resistant effect on cucumber angular spots, and the prevention effects are 56.59% and 60.43% respectively; the strain ZF535 exopolysaccharide has a remarkable induced disease-resistant effect on the cucumber soft rot disease through leaf spraying or root irrigation treatment, and the prevention effects are 54.35% and 65.11% respectively. In conclusion, the strain ZF535 is a simple pure bacillus with potential biocontrol application potential for inducing plant disease resistance, and has a better biocontrol effect.

The invention discloses a genetic transformation method of PEG (Polyethylene Glycol) mediated penicillium sclerotiorum strain protoplast and application of the genetic transformation method. The method comprises the following steps: (1) culturing penicillium sclerotiorum to produce spores, germinating hyphae, and collecting and cleaning fresh penicillium sclerotiorum hyphae; (2) compounding Lysing Enzymes, Yatase, cellulase and pectinase to prepare a protoplast enzymatic hydrolysate, and then adding the protoplast enzymatic hydrolysate into the penicillium sclerotiorum hypha for enzymolysis to obtain a penicillium sclerotiorum protoplast; and (3) adding plasmids to be transformed into the penicillium sclerotiorum protoplast, and carrying out PEG (Polyethylene Glycol) mediated transformation and resistance screening to obtain a penicillium sclerotiorum protoplast transformant. According to the method, the number and regeneration rate of the protoplast can be remarkably increased, and the enzymolysis time can be effectively shortened.

Disclosed herein are therapeutic compositions containing non-pathogenic, germination-competent bacterial spores, for the prevention, control, and treatment of gastrointestinal diseases, disorders and conditions and for general nutritional health.

The invention discloses a nucleic acid composition for detecting fungi as well as a kit and a detection method thereof, and relates to the technical field of molecular diagnosis. The embodiment of the invention provides a group of nucleic acid compositions, the compositions comprise primer pairs 1-5, the compositions can realize synchronous detection of aspergillus, cryptococcus, pneumocystis yarrowii and mucor on the basis of a real-time fluorescent quantitative PCR technology, and the nucleic acid compositions have the remarkable advantages of short detection time, simplicity and convenience in operation, high sensitivity, strong specificity and the like.

The application discloses a kind of broken wall ganoderma spore powder uncoating equipment and uncoating method, including shell, the shell is transverse barrel shape, the lower side of two end surfaces of the shell is fixedly installed with fixed plate, the bottom of two ends of two described fixed plate is fixedly installed with inclined support leg, the top of one of described fixed plate is fixedly installed with control module, the middle part of one end surface of the shell is installed with dry feeding mechanism that is connected with shell interior, breakage mechanism is installed in the shell, the broken wall ganoderma spore powder uncoating equipment and uncoating method can make ganoderma spore powder can quickly complete breakage, improve the efficiency of breakage, breakage rate can reach 98% or more, improve the edible taste of ganoderma spore powder, reduce difficult to digest and absorb component, nutritional value is higher, and the inner wall of first grinding barrel and second grinding barrel can be cleaned, so that ganoderma spore powder after breakage can be discharged to outside in whole, avoid the waste of ganoderma spore powder.

The invention relates to the technical field of microbial technology and rare earth element recovery. The invention provides bacillus tequilensis Re2, and the preservation number of the bacillus tequilensis Re2 is CGMCC (China General Microbiological Culture Collection Center) No. 36764. The strain Re2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 25, 2025, the center is called CGMCC for short, the address of the center is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number of the strain Re2 is CGMCC No.36764, and the strain Re2 is classified and named as Bacillus tequilensis. The bacillus tequilensis provided by the invention can efficiently adsorb various rare earth elements, and the rare earth elements in spores can be eluted after 2, 6-dipicolinic acid (DPA) is added; therefore, the repeated utilization of the strain is realized.

The invention discloses a primer probe group, a kit and a detection method for simultaneously detecting five fungi. Specifically, in the primer and probe group, the nucleotide sequences of the primers comprise SEQ ID NO.7 to 8, SEQ ID NO.16 to 17, SEQ ID NO.25 to 26, SEQ ID NO.34 to 35 and SEQ ID NO.43 to 44, and the nucleotide sequences of the probes comprise SEQ ID NO.9, SEQ ID NO.18, SEQ ID NO.27, SEQ ID NO.36 and SEQ ID NO.45. The invention also discloses a kit for detecting the detection of the tumor cells. According to the invention, multiple detection in one tube is realized, and five invasive fungi, namely mucor, aspergillus, cryptococcus neoformans, pneumocystis yarrowii and talaromyces marneffei, are simultaneously detected in one tube of reagent by utilizing a PCR method.

The invention belongs to the technical field of agricultural microorganisms, and relates to a bacillus velezensis strain S-34 and application thereof in prevention and treatment of mango leaf anthracnose, the bacillus velezensis strain S-34 is separated from rhizosphere soil of a mango variety 'Jinhuang' with high anthracnose resistance, is preserved in China Center for Type Culture Collection on May 29, 2025, and has a preservation number of CCTCC No. M 20251225. The bacillus velezensis S-34 provided by the invention not only can be used for preventing and treating mango leaf anthracnose, but also has a good antagonistic effect on various mango leaf pathogenic fungi. In addition, the bacillus velezensis S-34 as well as sterile fermentation liquor and volatile gas of the bacillus velezensis S-34 can inhibit spore germination and epidermis penetration processes of the colletotrichum siamensis, so that the infection process of the colletotrichum siamensis on plants is inhibited. The invention provides a novel antibiological inoculant and a preparation method for efficient prevention and treatment of mango leaf anthracnose and other leaf diseases in agricultural production, and is beneficial to green and healthy development of the mango industry.

Described are immunogenic compositions comprising a non-naturally occurring pan-fungal Kex peptide, and methods of using such compositions for the treatment or prevention of infection and / or diseases associated with fungal pathogens (e.g., Pneumocystis, Aspergillus, Candida, or Cryptococcus) in the subject. Also provided are compositions and kits for detecting or quantifying the presence of antibodies directed against a non-naturally occurring pan-fungal Kex peptide in a subject.

The invention discloses a cibotium barometz sporophyte transformation method and system based on multi-factor coupling regulation, and relates to the technical field of biology. The method comprises the following steps: synchronously acquiring environment data and sporophyte image data; preprocessing the environment data, performing feature extraction on the image data, and splicing the processed data into a comprehensive feature vector; quantifying the weight, correlation degree and interaction level among the environmental factors through an entropy method, grey correlation analysis and a coupling coordination degree model; constructing an improved CNN-LSTM neural network, extracting features through a double-branch structure, fusing a coupling analysis result to carry out cross-modal fusion, and training to obtain a prediction model; when the predicted conversion rate is lower than a threshold value, environment parameters are automatically adjusted until conversion is completed; according to the method, the problems of environmental factor data isolation and regulation lag in the prior art are solved, accurate prediction and intelligent regulation of the sporophyte transformation process are realized, and the transformation success rate and the production efficiency are remarkably improved.

The invention relates to recombinant bacillus subtilis for displaying PRRSV ER protein on spore surfaces, a construction method and application. The recombinant bacillus subtilis is obtained by constructing a recombinant vector which takes spore capsid protein CotB as anchoring protein and displays PRRSV ER protein on the surface by using a genetic engineering method on the basis of an integrated plasmid pDG364, and transforming the recombinant vector into wild bacillus subtilis 168 by using a chemical transformation method, and has genetic stability; and loss in the passage process is avoided. According to the strain, antigen protein is directly displayed on the surfaces of spores, crushing is not needed, animals can be immunized through direct material mixing or water drinking, and the tedious process of large-scale injection is avoided. And due to the good stress resistance of the spores, the strain is easy to store and transport, and the cost is remarkably reduced. The strain can induce specific immune response, provides a new way for prevention and treatment of PRRSV, and is suitable for development of commercial vaccines.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention provides a microbial agent for preventing and treating nematode diseases as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the microbial agent is mainly prepared from the following raw materials in percentage by mass: 60-75% of microbial thalli; 25%-40% of an additive; wherein the microbial thalli are prepared from purpureocillium lilacinum, bacillus subtilis, brevibacillus laterosporus, bacillus mucilaginosus and lactobacillus sporogenes. Through the collaborative design of a specific strain combination and a functional additive, the stress resistance and colonization ability of the microbial inoculum in a complex soil environment can be effectively improved, meanwhile, an efficient synergistic mechanism of fungi and bacteria is established, the microbial inoculum can adapt to complex soil types, and the control effect on nematodes can be effectively improved. The invention fundamentally solves the industrial obstacles of poor environmental adaptability, short lasting period, single function and the like of biological agents, and provides a solution for large-scale application for chemical pesticide substitution.

The invention discloses a method for improving the spore concentration of paenibacillus polymyxa, which comprises the following steps: culturing paenibacillus polymyxa, and adding a carbon source and calcium carbonate into a culture solution at one time or multiple times in the logarithmic phase of the culture. The invention provides a method for improving the spore concentration of paenibacillus polymyxa. Compared with an original culture technology, a glucose and calcium carbonate combined method is adopted in the logarithmic phase of the paenibacillus polymyxa, glucose is added to obviously improve the thallus concentration, calcium carbonate is added to obviously promote spore formation, and the spore concentration of the paenibacillus polymyxa is obviously increased; the spore concentration in the production process of the paenibacillus polymyxa as an industrial biological control strain is remarkably improved.