9 results about "UV-Mutagenesis" patented technology

The invention relates to the technical field of environmental microorganisms and resource fermentation, and discloses a Kluyveromyces marxianus BMK7 for protein production and biogas slurry purification and application of the Kluyveromyces marxianus BMK7, the BMK7 is Kluyveromyces marxianus and is obtained through ultraviolet mutagenesis and hygromycin B adaptive evolution, the preservation number is CCTCC NO: M 20251050, and the Kluyveromyces marxianus BMK7 has good growth performance and genetic stability. The strain can be efficiently fermented under the condition that biogas slurry serves as a main substrate, ammonia nitrogen and chemical oxygen demand (COD) in the biogas slurry are remarkably reduced, and meanwhile high-proportion mycoprotein is synthesized. The thalli have strong flocculability and good sedimentation characteristics, and are beneficial to protein recovery. The method can be widely applied to treatment and resource utilization of sewage such as breeding wastewater, kitchen fermentation liquor and anaerobic effluent, and has a good industrialization prospect.

The invention relates to the technical field of microorganisms, in particular to a beta-1, 4-endo-xylanase gene BsxynA and application of the beta-1, 4-endo-xylanase gene BsxynA. According to the application, the beta-1, 4-endo-xylanase gene BsxynA is screened and identified, in-vitro induced expression is performed on the beta-1, 4-endo-xylanase gene BsxynA for the first time, and enzymatic properties of the beta-1, 4-endo-xylanase gene BsxynA are researched The enzyme activity of the bacillus safensis xylanase reaches 83.913 U / mL, the bacillus safensis xylanase is expected to be subjected to gene mutation through mutation breeding such as physical (such as ultraviolet rays and gamma rays) or chemical (such as nitrosoguanidine and diethyl sulfate) mutagenic agent treatment, and bacillus safensis mutants with higher enzyme activity are directionally screened. For example, ultraviolet mutagenesis can cause random mutation of the DNA of the microorganism, change the expression of the gene or the structure of the zymoprotein, and possibly increase the xylanase yield or enhance the xylanase activity. The produced BsXynA mainly promotes the hydrolysis of beech xylan, so that xylobiose (X2) and xylotetraose (X4) are generated, and xylose is not generated. In addition, the BsXynA significantly improves the digestive performance of the animal feed, and can increase the xylose content in the feed, promote the nutrient absorption of animals and reduce the production cost.

The present disclosure provides a mutant strain of Aspergillus niger designated MTCC 25761 on deposit with the Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh, derived from parent strain Aspergillus niger ATCC 9142 through UV mutagenesis at 254 nm wavelength for 19 minutes with constant stirring The mutant strain demonstrates enhanced production capabilities for both citric acid and gluconic acid compared to the parent strain. The mutant strain exhibits enhanced metabolic performance through improved enzyme expression, substrate utilization efficiency, and regulatory control mechanisms. Gluconic acid production is 1.37-fold higher (164.045 g / L) and citric acid production is 1.29-fold higher (0.506 g / L) than the parent strain.

The invention relates to the technical field of microorganisms, and discloses bacillus velezensis inducing bacteria and application thereof in lipopeptide production, the bacillus velezensis is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 6, 2022, and the preservation number is CGMCC No.24640. The invention further discloses a preparation method of the bacillus velezensis inducing bacteria. According to the invention, the bacillus velezensis is taken as an original strain, and a mutant strain with high yield of lipopeptide is successfully screened out through the technical means of ultraviolet mutagenesis, preliminary screening, secondary screening and the like, so that the yield of lipopeptide is remarkably increased. Besides, the chelating capacity of the produced lipopeptide and metal ions is evaluated, the result shows that the binding content of zinc ions in the chelate reaches 478.2012 mg / 100g, and further bacteriostatic experiments show that the chelate can effectively inhibit growth of micrococcus luteus.

The invention belongs to the technical field of genetically engineered bacteria, and particularly discloses apramycin genetically engineered bacteria with genetic stability as well as a construction method and application of the apramycin genetically engineered bacteria. The construction method comprises the following steps: by taking a plasmid containing an xylE gene and a tsnR gene as a template, obtaining a linearized vector skeleton without a promoter; the method comprises the following steps: inserting a streptomyces indicus promoter sequence into a linearized vector skeleton to enable the promoter to be located at the upstream of an xylE gene and a tsnR gene, and constructing a double-reporter gene expression vector; transferring the expression vector into streptomyces through conjugation to obtain a conjugate; the genetically stable strain is obtained through ultraviolet mutagenesis in combination with catechol chromogenic screening. The biological value is increased by more than 40% compared with that of an original strain, the value retention rate of 94.5% or above is kept in continuous 10 generations of long-term passage experiments, and a powerful technical support is provided for industrial stable production of anti-mycoplasma active substances.

The application discloses an escherichia coli zjut-mhol and application thereof in production of heme oxygenase-1, and a gene of mouse-derived HO-1 is synthesized and cloned by using a preferred codon optimization method, a gene recombinant escherichia coli capable of producing HO-1 is constructed, and a mutant strain with high enzyme activity and stable passage is obtained through ultraviolet mutagenesis, and the mutant strain can be applied to preparation of HO-1. The strain is cultured for enzyme production, and the bacterial body with intracellular active HO-1 is obtained, and the enzyme production activity is 122 U / g according to the mass of the dry bacterial body. The HO-1 freeze-dried powder is separated from the bacterial body, and the enzyme activity of the freeze-dried powder is 91.8 U / g.

Disclosed herein is a UV mutagenesis produced new Streptomyces spororaveus strain SC263 that exhibits an increased thermostability of the antibiotic produced, which shows antibiotic activity against bacteria and fungi. Also provided are the antibiotic produced by SC263 and a composition thereof for treatment of bacterial and fungal infections. The antibiotic has a very high molecular weight, which is suitable for topical administration.

The invention relates to a Candida ballonica for high yield of sophorolipid through a one-step conversion method, and belongs to the technical field of sophorolipid synthesis, the Candida ballonica is a strain obtained by sequentially carrying out ARTP mutagenesis and UV mutagenesis on Starmeria bombica ATCC 22214, the classification name of the strain is Candida ballonica U1-4, the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the strain is CGMCC NO. The preservation address is No. 299 on eight road, Wuchang District, Wuhan, Hubei, China, the preservation number is CCTCC NO: M 2025692, and the preservation date is April 3, 2025. The mutant strain U1-4 obtained in the invention has excellent tolerance to phenolic aldehyde inhibitors, and inhibitors of specific types and concentrations generated in a straw system pretreated by NaOH are suitable for growth and fermentation of the mutant strain U1-4 in the process of a one-step conversion method, so that inhibition of the inhibitors on growth and fermentation of microorganisms is reduced, and the yield of the mutant strain U1-4 is improved. And a phenolic aldehyde inhibitor can be used as a carbon source for growth and fermentation, so that the yield of sophorolipid is increased and reaches 76.46 g / L.

The invention belongs to the technical field of biological fermentation, and particularly relates to Wickerham Schiff's yeast with high yield of triacetyl phytosphingosine and application of the Wickerham Schiff's yeast. The strain is Wickerhamomyces ciferrii DB38MQ9, is preserved in the China Center for Type Culture Collection, and has the preservation number of CCTCC (China Center for Type Culture Collection) M20251536. The strain is obtained by performing ARTP-UV mutagenesis and screening on an initial strain of Wickerham Schiff's yeast for producing tetra-acetyl phytosphingosine, the fermentation level of the tetra-acetyl phytosphingosine in fermentation liquor can be effectively improved, the content of the tetra-acetyl phytosphingosine in the fermentation liquor of the strain can reach nearly 30g / L in 100L tank fermentation, and the yield of the tetra-acetyl phytosphingosine can be greatly improved. Not only is the subsequent purification process of the triacetyl phytosphingosine simplified, but also the method has certain application value and guiding significance for reducing the production cost of the phytosphingosine and improving the productivity.