86 results about "UV-Mutagenesis" patented technology

The invention discloses abamectin producing bacterium and a preparation method thereof. The collection name of the bacterium is FT26-9 and the collection number of the bacterium is CGMCC No.4341. The bacterium was collected on November 12th, 2010. The preparation method comprises: performing ultraviolet and lithium chloride combined mutation induction treatment of a starting strain; screening an induced-mutation strain; performing mutation induction by nitrosoguanidine and screening; performing mutation induction by diethyl sulfate and screening; performing mutation induction by hydroxylamine and screening; performing mutation induction by 5-bromouracil and ultraviolet and screening; and finally obtaining the FT26-9 strain which can greatly improve the abamectin B component content in a fermentation product and reduce the abamectin A component content in the fermentation product. When the bacterium is used in industrial production of abamectin, the fermentation unit is improved by about 40 percent, and the product cost is reduced by about 35 percent.

The invention discloses fermentable silk spore yeast strain and a method for preparing microbial oil with lignocellulose hydrolyzate. The method for preparing the yeast strain comprises the following steps of: taking the fermentable silk spore yeast CICC 1368 as the original strains, performing the ultraviolet ray mutagenesis and the composite mutagenesis of the ultraviolet ray and the LiCl, obtaining the high-yield strains by means of fermenting and screening, and naturalizing the obtained strains in the lignocellulose hydrolyzate to finally obtain the oil yeast strain CCTCC M209060 which can effectively accumulate the oil in the lignocellulose hydrolyzate. The strains has high oil yield compared with the original strains, can effectively accumulate the oil in the lignocellulose hydrolyzate which contains the inhibitor with higher concentration, and offers the probability for preparing the microbial oil with low cost, high efficiency and large scale.

The invention discloses a bacterial strain for producing fructosyl transferase and a preparation method thereof. The bacterial strain disclosed herein is prepared by the following steps: firstly screening a bacterial strain by lithium chloride and UV mutagenesis, and then changing the property of fructosyl transferase in the bacterial strain by genome shuffling. The bacterial strain is named Aspergillus oryzae scut209, preserved in China Center for Type Culture Collection (CCTCC) in Wuhan, China, on April 10, 2012 with CCTCC Designation CCTCC No:M 2012106. The bacterial strain has high capability of producing fructosyl transferase and has high industrial application value, wherein the capability of producing fructosyl transferase reaches 34987U/g cell.

The invention discloses a preparing method of bacillus subtilis TQS6 CCTCC M207001 of highly effective expression neutral beta-mannase through adopting UV (ultraviolet mutagenesis ) and NTG (nitrosoguanidine mutagenesis) compositing mutagenesis, which comprises the following steps: bevel-culturing bacterial strain; proceeding ferment culture after one grade and two grade culture; choosing culture medium; adding into not exclusively degraded liquid from centrifuging sugar liquid; venting into oxygen-enriched air during the course of yeast; increasing dissolved oxygen; getting high active neutral beta-konjak mannase. The product possesses high enzyme production, high enzyme activator, short period of production and low cost, which can invert low mannan effectively.

The present invention is production process of UV and NTG mutagenizing Bacillus subtilis TQS6 to culture high efficiency expressing chitosanase strain TQK and fermentation producing high activity chitosanase. The chitosanase strain is first slant cultured and then fermentation cultured while introducing oxygen-rich air to increase dissolved oxygen to 30-80 %; and after culturing for 18-24 hr, average enzyme activity may reach 718 mu/ml. High activity chitosanase may be obtained through fermentation in optimized enzyme producing condition. The process of the present invention can obtain chitosanase with high yield, high stability and high activity, and the produced chitosanase is applied in converting chitosan into chitin oligose effectively and economically.

The present invention relates to Bacillus subtilis Fds-1 with the preservation number of CGMCC No. 0644. The strain of the present invention is obtained through separation from the soil near high sulfur oil well, ultraviolet mutagensis and screening. The growing cell and the rest cell of the strain can make the C-S bond oxidized to break and thus eliminate organic sulfur from organic compound, so that the strain is especially suitable for eliminate organic sulfur from petroleum fractions in high desulfurizing rate.

The invention relates to an endogenous bacillus subtilis positive mutant strain, a preparation method thereof, a prepared biocontrol agent and an application of the biocontrol agent in preventing and controlling pomegranate dry rot, including (1) screening of a Bacillus subtilis wild-type strain NS04 and ultraviolet mutation and breeding of a positive mutant biocontrol strain NS04307; (2) antibacterial stability of the positive mutant strain NS04307; (3) a preparation method of the positive mutant strain NS04307 biocontrol agent; and (4) application of the positive mutant strain NS04307 in preventing and controlling plant diseases. The biocontrol agent disclosed by the invention has the characteristics of wide bactericidal spectrum, good effect, difficulty in disease drug resistance generation, safety on human, livestock and crops, environmental friendliness and the like and has a better effect on the field control of the pomegranate dry rot especially. In addition, the biocontrol agent disclosed by the invention achieves outstanding inhibition activity on the growth of 15 different pathogenic bacteria, such as Sclerotiumrolfsii, Trichotheciumroseum, Exserohilumrostratum, Fusariumoxysporum, Alternariaalternate and the like.

The invention belongs to the technical field of bioengineering, and particularly discloses bacillus circulans and an application of the same in preparation for ferulic acid decarboxylase. The bacillus circulans is characterized in that a bacillus circulans HG12 strain is obtained by separating from a high-temperature yeast for brewing Maotai-flavour liquor in the white wine plants of China, and screening by virtue of ultraviolet mutation, as well as has the characteristics of being fast in growth and propagation speeds, capable of producing the ferulic acid decarboxylase under the induction of ferulic acid, stable in performance of producing the ferulic acid decarboxylase, and high in enzymatic activity. According to the bacillus circulans and the application disclosed by the invention, continuous and large-scale production for the ferulic acid decarboxylase can be realized, and industrialized production for the ferulic acid decarboxylase is facilitated; moreover, the enzyme is simple and convenient in production process, short in production cycle, and low in cost.

The invention discloses Monascus anka Yang YZ 301 and a cultivation method thereof, wherein the microbial preservation number of the Monascus anka Yang YZ 301 is CGMCC9707. The invention further discloses a method for preparing lower citrinin and high color value monascus red pigment through liquid state fermentation of the Monascus anka Yang YZ 301. The method includes the first step of strain breeding, the second step of seed cultivation and enlarged cultivation, and the third step of liquid state fermentation, extraction and purification, spray drying and the like. The strains are the Monascus anka Yang YZ 301 obtained by cultivation, repeated UV mutagenesis and repeated isolation and screening of Monascus anka Nakazawa et Sato YZ201 developed by the Tianyi Biological Fermentation Technology Co., Ltd in Dongguan. The invention further discloses a lower citrinin and high color value monascus red pigment product obtained through the preparing method. The provided strains are excellent, and the technology is reasonable and stable. The obtained product is high in yield and stable in quality. The content of citrinin in the product is not more than 0.2 mg/kg (calculated by unit color value of 500 mu/g), and the color value of monascus red pigment ranges from 10,300 mu/g to 10,800 mu/g.

The present invention provides a high-yielding astaxanthin mutant strain of Haematococcus pluvialis (Haematococcus pluvialis) E1 CCTCC M 207164, which uses the wild strain of Haematococcus pluvialis as material , using ultraviolet chemical compound mutagenesis, mutagenesis selection of carotenoid biosynthesis inhibitor screening. The astaxanthin content of the algal strain is 1.83 times higher than that of the original strain, the growth rate is 14% higher than that of the original strain, the physiological and biochemical characteristics are stable, and it has stable genetic properties. It is an excellent algal strain for producing natural astaxanthin and can be used as a source of industrial production. Industrial production of natural astaxanthin.

The present invention relates to saline algae, provides one kind of low temperature tolerant saline algae and its ultraviolet mutagenesis breeding process and identifying method. The low temperature tolerant saline algae features its light illuminating growth at 3-10deg.c to reach (1.05-8.11)x10<6> cell/ml in the cell density 2.1-21 times higher than the contrast; the genetic similarity coefficient to wild strain of 0.680-0.910; and increased or decreased protein zone(s) compared with wild strain.

The invention discloses torulaspora delbrueckii and an application thereof. A preservation number of torulaspora delbrueckii is CGMCC No.8740 and CGMCC No.8742. An ultraviolet mutation method is adopted to mutate torulaspora delbrueckii; then mutated strains having high active phytase under an acid condition are screened out, so that mutated phytase produced by the mutated strains can hydrolyze phytic acid under pH conditions of a stomach environment relatively well. The mutated strain can be used for producing feed or food phytase or producing microbial phosphate fertilizer or bioorganic fertilizer. Besides, the mutated phytase gene sequence can be cloned to prokaryotic or eukaryotic gene expression vectors to construct gene engineering strains or transgenic plants by gene engineering technology, so as to efficiently express the mutated phytase genes, thereby producing the mutated phytase.

The invention relates to an inhibitor-resistant saccharomyces cerevisiae and a selective culture method and application thereof. The inhibitor-resistant saccharomyces cerevisiae is named as ZR/SC-UV-2, classified and named as saccharomyces cerevisiae, and deposited in the China general microbiological culture collection center, the preservation number is CGMCCNo.17924, and the preservation addressis No.3, yard 1, Beichen west road, Chaoyang district in Beijing. The selective culture method includes the following steps that the saccharomyces cerevisiae of xylose can be used as a starting strain, and the inhibitor-resistant saccharomyces cerevisiae is obtained after UV-induced screening. The UV mutagenesis condition includes the following steps that under a light-shading condition, a UV lamp of 15-20 W is used for irradiating for 30-600 seconds at a distance of 15-20 cm for UV mutagenesis. Compared with the prior art, the saccharomyces cerevisiae has strong resistance to an inhibitor incellulose hydrolysate, the degradation rate of the xylose is high, the fermentation time is short, and the system is suitable for the industrialized production of ethanol prepared from agricultural straw.

The invention discloses a method for screening a methionine resistant deinsectization streptomyces avermitilis strain, which belongs to the field of the screening of microbial strains. The method comprises the following steps of: performing ultraviolet mutagenesis on deinsectization streptomyces avermitilis spore suspension; performing enrichment on tolerant strains by using a methionine-containing liquid culture medium; screening methionine solid-plate tolerant strains; and screening strains of which the shake flask capacity is improved compared with that of parent strains through preliminary screening, secondary screening and genetic stability research so as to provide a feasible method for screening the deinsectization streptomyces avermitilis strain. The shake flask production capacity of the deinsectization streptomyces avermitilis strain screened by the method is greatly improved, and the genetic stability is high.

The invention discloses an androstenedione substrate-tolerant mutant strain and a mutation breeding method thereof. A mutant strain of mycobacterium neoaurum WS3 is preserved in China center for type culture collection with an accession number of CCTCC NO: M 2014322, and the preservation date is July 4, 2014. According to the invention, mycobacterium neoaurumJXNU02 is used as a starting strain; compound mutation is carried out by diethyl sulfate and an ultraviolet mutation method; primary screening is performed by a gradient plate method; secondary screening and verification are carried out by a shake flask fermentation method; and thus the mutant strain of mycobacterium neoaurum WS3 with high substrate tolerance and genetic stability is bred. The method of the invention effectively improves the capability of mycobacteria for degrading phytosterol to generate androstenedione, provides an excellent strain for producing androstenedione by a microbiological method, and has important significance on the pharmaceutical industry of our country.

The invention discloses a method for increasing the antibiotic yield by virtue of mutagenesis of an erythromycin producing strain. The method comprises the following steps: (1) pre-culturing the erythromycin producing strain used as a starting strain on a slant culture medium for 6-7 days; (2) carrying out ultraviolet mutagenesis on the pre-cultured erythromycin producing strain, namely carrying out mutagenesis through irradiation for two minutes with a 15W ultraviolet tube under the condition that the fixed distance is 20cm and coating a culture medium plate immediately after mutagenesis; (3) carrying out ion beam irradiation mutagenesis on a viable bacterial colony after mutagenesis, namely carrying out preliminary screening with the culture medium plate after mutagenesis and carrying out secondary screening by a shake flask fermentation method, wherein the mutagenesis temperature is 25 DEG C and the mutagenesis dose is 50Gy; the obtained viable bacterial colony is the high-yield mutant erythromycin producing strain. The high-yield mutant erythromycin producing strain is obtained by adopting the method. Experimental results prove that the antibiotic yield of the mutant erythromycin producing strain after mutagenesis treatment is increased; the tank release titer of a 100L fermentation tank is 15600u/ml; the content of a component A in the fermentation liquor is increased from original 72% to 85% and the content of a component B is reduced from original 2.7% to 1.1%, thus bringing great convenience for subsequent treatment of the fermentation liquor; the product quality and purity are improved; the production cost is reduced.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

A method for mutagenizing and selectively culturing a refractory single-cell green alga includes such steps as sterilizing and cooling culture medium, inoculating alga liquid, culturing, mixing with iodine solution or bromophenol blue, deactivating, calculating alga density in alga liquid, ultraviolet mutagenizing, dark culturing, mixing the cultured alga liquid with fresh culture medium, culturing, high-temp screening, amplifying culture of living alga cells, culturing under light radiation, inoculating yellow alga, culturing, testing its mutagenized effect, measuring the long and short diameters of cell, extracting DNA, random amplifying of polymorphic DNA, calculating genetic similarity coefficient, extracting H-42 and general protein, electrophoresis, dyeing with Coomassic brilliant blue, recording result and observing the electropherogram to obtain result.

The invention discloses a method for improving cadmium tolerance of bacillus through mutation, which comprises the following steps of: (1) taking bacillus subtilis as a starting bacterium, and pre-culturing the bacillus on a beef extract peptone culture medium for 10 hours; and (2) performing ultraviolet mutation on pre-cultured active colony: irradiating by using 15W ultraviolet lamp under the condition of a fixed distance of 30cm for 5 minutes, performing mutation, screening by fermenting on a concentration gradient culture medium plate and in a shake flask, wherein the obtained active colony is the mutated bacillus with high tolerance to cadmium. Through the method, the mutated bacillus B38 fully tolerating high concentrate cadmium is obtained, and the cadmium tolerant concentration is 3mmol/L.

The invention relates to a succinic acid-rich strain for brewing soy sauce as well as a mutation breeding method and application of the succinic acid-rich strain. The strain is preserved in China General Microbiological Culture Collection Center on June, 2016, with a preservation number of CGMCC No.12629. The mutation breeding method comprise the steps of firstly preparing Aspergillus oryzae Huniang 3.042 spore suspension, preparing a spore protoplast, carrying out ultraviolet mutation, coating culturing, preliminary screening, secondary screening and genetic stability research, and applying the obtained product to the preparation of soy sauce finished koji in a soy sauce brewing process. Compared with the prior art, the strain for brewing the soy sauce has the advantages that the genetic property is stable, and a large number of succinic acid can be produced during the fermentation of the soy sauce, so that the concentration of succinic acid in the finished soy sauce is substantially increased, the delicate flavor of the finished soy sauce is obviously improved, and the quality of the finished soy sauce is improved; the mutation breeding method is simple, the operation is simple, the input cost is low, the soy sauce finished koji prepared from the strain has high succinic acid content and good enzymatic activity, and the sense and the quality of the finished soy sauce prepared from the strain can be obviously improved.

The invention discloses a method for improving the tolerance of erythromycin-producing strain against n-propanol through mutagenesis. The method comprises the steps that: (1) an erythromycin-producing strain is adopted as an initial strain, and is pre-cultured for 7 days on a slant culture medium; (2) the pre-cultured erythromycin-producing strain is subjected to ultraviolet mutagenesis, wherein the strain is irradiated for 3min under conditions of an ultraviolet lamp with a power of 15W and a fixed distance of 20cm; and the obtained product is instantly smeared on a culture medium plate; (3) live colonies after mutagenesis are subjected to ion beam irradiation mutagenesis, wherein a mutagenesis temperature is 25 DEG C and mutagenesis dose is 55Gy; after mutagenesis, culture medium plate pre-screening and shaking flask fermentation secondary-screening are carried out, and the obtained live colonies are the mutant erythromycin-producing strain with tolerance to n-propanol. With the method, the mutant erythromycin-producing strain with tolerance to high-concentration n-propanol is obtained. The n-propanol tolerance concentration is higher than 2%. Therefore, erythromycin-producing strain tolerance against n-propanol is improved, such that n-propanol concentration in a culture medium can be increased. Under a condition that producing strain conversion rate is unchanged, with the increasing of n-propanol concentration, erythromycin yield is increased. A draw-off titer of a 100L fermentation tank can be higher than 13000u/ml.