87 results about "UV-Mutagenesis" patented technology

The invention discloses a method for increasing the antibiotic yield by virtue of mutagenesis of an erythromycin producing strain. The method comprises the following steps: (1) pre-culturing the erythromycin producing strain used as a starting strain on a slant culture medium for 6-7 days; (2) carrying out ultraviolet mutagenesis on the pre-cultured erythromycin producing strain, namely carrying out mutagenesis through irradiation for two minutes with a 15W ultraviolet tube under the condition that the fixed distance is 20cm and coating a culture medium plate immediately after mutagenesis; (3) carrying out ion beam irradiation mutagenesis on a viable bacterial colony after mutagenesis, namely carrying out preliminary screening with the culture medium plate after mutagenesis and carrying out secondary screening by a shake flask fermentation method, wherein the mutagenesis temperature is 25 DEG C and the mutagenesis dose is 50Gy; the obtained viable bacterial colony is the high-yield mutant erythromycin producing strain. The high-yield mutant erythromycin producing strain is obtained by adopting the method. Experimental results prove that the antibiotic yield of the mutant erythromycin producing strain after mutagenesis treatment is increased; the tank release titer of a 100L fermentation tank is 15600u/ml; the content of a component A in the fermentation liquor is increased from original 72% to 85% and the content of a component B is reduced from original 2.7% to 1.1%, thus bringing great convenience for subsequent treatment of the fermentation liquor; the product quality and purity are improved; the production cost is reduced.