1 results about "Nitrosoguanidines" patented technology

This invention discloses a strain of *Bacillus coagulans* that facilitates cell recovery and its application in lactic acid fermentation, belonging to the fields of microbiology and fermentation engineering technology. This invention isolates a batch of thermoresistant bacteria from spoiled milk. After small-scale cell culture, a flocculant is added to observe sedimentation. The strain with the fastest sedimentation rate is selected, and *Bacillus coagulans* is identified among them. Further screening yields a strain, XQ20249, with rapid cell sedimentation and high optical purity of L-lactic acid produced during fermentation. Further mutagenesis using nitrosoguanidine and screening yields mutant strains with even faster cell sedimentation. Among them, mutant strain LL08 shows a significant increase in flocculation and exhibits faster growth and lactic acid fermentation rates than other mutant strains under lactic acid fermentation conditions. When strain LL08 is used for lactic acid fermentation, the cells can be collected by sedimentation after fermentation for secondary fermentation, significantly reducing the amount of organic nitrogen source used.