Method for improving saccharopolyspora spinosa spinosad fermentation yield

Abstract

The invention relates to a method for improving a saccharopolyspora spinosa spinosad fermentation yield. The method utilizes mutagenesis treatment to screen a saccharopolyspora spinosa spinosad high-yield mutant strain with a good starch utilization capability. The mutagenesis treatment comprises at least two of UV-streptomycin combined mutagenesis, 5-fluorouracil mutagenesis, microwave-streptomycin combined mutagenesis and nitrosoguanidine-streptomycin combined mutagenesis. Preferably, the mutagenesis treatment comprises the above four mutagenesis methods. Preferably, the UV-streptomycin combined mutagenesis, 5-fluorouracil mutagenesis, microwave-streptomycin combined mutagenesis and nitrosoguanidine-streptomycin combined mutagenesis processes are orderly carried out. The broth of the obtained mutant strain has spinosad titer substantially higher than that of an original strain screened through mutagenesis and thus the method can improve a saccharopolyspora spinosa spinosad fermentation yield.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for increasing the spinosad fermentation yield of Saccharopolyspora spinosa. Background technique [0002] Spinosyn, a secondary metabolite produced by the aerobic fermentation of Saccharopolyspora spinosa, belongs to macrolide compounds, and the effective active ingredient in its commercial products is spinosad A (85%-90%) and spinosyn D (10%-15%). Spinosad has no antibacterial activity, but it has good insecticidal activity. It has a wide insecticidal spectrum against pests of Lepidoptera and Thysanoptera. Lepidopteran pests have a good control effect. Spinosad is one of the most selective compounds among existing insecticides in the control of Lepidoptera pests, and its activity is equivalent to cypermethrin. While spinosyn has high insecticidal activity, it exhibits low toxicity to non-target organisms. It has low toxicity to mammals, birds and beneficial ins...

AI Technical Summary

Problems solved by technology

In the process of using Saccharopolyspora spinosa to ferment spinosad, starch is often added to the medium as a late-acting carbon source because of its low price, wide source, and ability to relieve the glucose effect. In production practice , the existing Saccharopolyspora spinosa used to produce spinosad generally has the defect of low utilization of starch in the fermentation medium, and polysaccharides that are not effectively utilized often remain in the fermentation broth, resulting in the final obtained fermentation broth It is very viscous, which increases the difficulty of extraction of spinosad in the later stage and increases the waste of resources

Importantly, strains with poor starch utilization were also defective in low spinosyn fermentation yields

Images