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87 results about "Starch hydrolysis" patented technology

Starch Hydrolysis Starch agar is a differential medium that tests the ability of an organism to produce certain exoenzymes, including a-amylase and oligo-1,6-glucosidase, that hydrolyze starch.

Method for improving conversion efficiency of fermentation protein for cyperus esculentus feed and prepared fermentation protein for cyperus esculentus feed

The invention provides a method for improving the conversion efficiency of fermentation protein for a cyperus esculentus feed and the prepared fermentation protein for the cyperus esculentus feed. Comprising the following steps: uniformly mixing cyperus esculentus cake meal, chitosan oligosaccharide and water, inoculating a fermentation strain, and culturing under proper conditions. Wherein the fermentation strain comprises saccharomycetes or a mixed strain of saccharomycetes and aspergillus niger; the saccharomycetes comprise saccharomyces cerevisiae or a mixed strain of the saccharomyces cerevisiae and candida utilis saccharomycetes. Saccharomyces cerevisiae is used as a main fermentation strain, sugar in fermentation raw materials can be fully utilized, mycoprotein is generated, and the protein content is increased; the candida utilis can utilize various carbon sources and nitrogen sources, so that the protein content and the thallus biomass are further improved; aspergillus niger can generate saccharifying enzyme, starch is hydrolyzed into glucose and other simple saccharides, and the saccharifying process is promoted. According to the method provided by the invention, the cyperus esculentus raw material with relatively low protein content can be converted into the cyperus esculentus feed fermentation protein, and the utilization way of the cyperus esculentus is expanded.
Owner:ZHONGGUANCUN RAND INST OF SCI & EDUCATION INNOVATION

Phyllanthus emblica polysaccharide with sugar-controlling and lipid-lowering activity as well as preparation method and application of phyllanthus emblica polysaccharide

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.
Owner:SOUTH CHINA UNIV OF TECH

Raw amylolytic enzyme mutant as well as expression strain and application thereof

The invention discloses a raw amylolytic enzyme mutant as well as an expression strain and application thereof. According to the present invention, alpha-amylase AmyZ1 from Pontibacillus sp.ZY is adopted as a starting enzyme, a substrate binding subsite is determined through molecular docking, and site-directed mutagenesis is performed on residues at a + 1 site and at a 199 site so as to obtain the mutant enzyme L199F with high glucose yield; when the mutant enzyme is used in an industrial sugar manufacturing process, corn raw starch is used as a substrate, and the DE value can reach 35% after liquefaction for 2 h and is 1.44 times that of a starting enzyme; after saccharification for 18 hours, the DE value can reach 99%, which is 5 hours earlier than that of the starting enzyme. Therefore, the mutant enzyme has relatively high potential application value in the field of sugar industry taking corn raw starch as a substrate.
Owner:ANHUI UNIV

Nutrition-balanced, sugar-controllable, fat-reducing and staple food nutrition-improving medicated food and preparation method thereof

The invention discloses nutrition-balanced, sugar-controllable, fat-reducing and staple food nutrition-improving medicated food and a preparation method thereof. The medicated food is prepared from the following raw material components: 5-10% of high-quality protein, 1-10% of high-quality fat-containing food raw materials, 0.8-15% of medicinal and edible traditional Chinese medicines, 5-15% of high-quality carbohydrates, 5-60% of dietary fibers, 2-10% of functional sugar and 1-2% of minerals. The formula food is directly cooked with rice to form rice and porridge or is directly mixed with flour to form cooked wheaten food, so that the contact degree of starch and digestive enzyme and the activity of glycosidase can be reduced, the starch hydrolysis rate can be reduced, and the generation of postprandial blood sugar can be delayed. The formula food is prepared from various functional food raw materials and medicinal and edible food materials, is safe and free of side effects, and can be taken for a long time. All the components supplement each other to achieve a synergistic effect, the glycemic index of a patient can be adjusted, diabetic complications of the patient are improved, and therefore disease rehabilitation of the diabetic patient is promoted.
Owner:张林昌

Boiling-water-resistant flame-retardant starch-based wood adhesive as well as preparation method and application thereof

The invention belongs to the technical field of starch-based adhesive materials, and provides a boiling-water-resistant flame-retardant starch-based wood adhesive as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing starch, water and alpha-amylase, and performing hydrolysis reaction to obtain a starch hydrolysate; mixing the starch hydrolysate and the DIHP aqueous solution, and reacting to obtain an S-DIHP material; and mixing the nano hybrid DE (at) TA with an S-DIHP material to obtain the boiling water-resistant flame-retardant starch-based wood adhesive. The adhesive provided by the invention is prepared through an enzyme catalysis hydrolysis strategy and a hyperbranched cross-linked network strategy, and has excellent boiling water resistance; by adding the nano hybrid DE (at) TA taking polysiloxane as a core and tannic acid as a shell, not only is the adhesive property of the adhesive improved, but also the flame retardance of the adhesive is remarkably enhanced, and the adhesive is suitable for the wood processing industry and is used for replacing a traditional petroleum-based adhesive, so that the environmental pollution is reduced.
Owner:SOUTHWEST FORESTRY UNIVERSITY

Low-glycemic-index steamed bun premixed flour and steamed bun product making method

The invention belongs to the field of functional food, and discloses steamed bun premixed flour with a low glycemic index and a steamed bun product making method, and the premixed flour is composed of whole wheat flour, resistant starch (RS2 type), ultramicro highland barley flour (beta-glucan is greater than or equal to 5%), bean flour (chickpeas and Chinese yams), momordica grosvenori concentrated juice, mulberry leaf high-fiber powder, compound lactic acid bacteria powder and the like. The preparation method comprises the processes of dry-mixing premixed flour, staged temperature-controlled fermentation (35-38 DEG C to 28-30 DEG C), gradient cooling steaming (2-3 DEG C / min to 1 DEG C / min) and the like. Through the high-temperature gelatinization characteristic (85-95 DEG C) of the RS2 type resistant starch and the synergistic effect of the highland barley flour and the mulberry leaf fibers, the starch hydrolysis index is remarkably reduced to be smaller than or equal to 40%, and the eGI value is smaller than or equal to 55. The dietary fibers of the product are not less than 7g / 100g, the proteins are not less than 12g / 100g, the texture hardness is not more than 550N, and the elasticity is not less than 2.5 mm. Clinical experiments show that the postprandial blood sugar peak value of diabetic patients is reduced by 28%, and the composition is suitable for diet management of diabetic and obesity patients. The food has low GI, high nutrition and palatability, and solves the technical problems of fast sugar rise and insufficient fibers of traditional staple food.
Owner:SUZHOU YIJIANGNAN FOOD CO LTD

A Stability-Enhanced Starch Hydrolase Mutant and Its Application

This invention discloses a mutant of raw starch hydrolase with improved stability and its applications. Using α-amylase AmyZ2 as the starting enzyme, and through computer-aided design to clarify the mutation site, a mutant was heterologously expressed in Bacillus subtilis, resulting in an α-amylase with improved stability. When using raw corn starch as a substrate, the mutant exhibits 1.57 times the specific enzyme activity and 2 times the stability of the starting enzyme. While maintaining the specific enzyme activity, the mutant enzyme shows a significant improvement in thermostability. This mutant has potential application value in the production of starch sugars using raw corn starch as a substrate.
Owner:ANHUI UNIV

A highland barley sourdough, a low-gi highland barley sourdough bread and a preparation method thereof

The application belongs to the technical field of food processing, and specifically discloses a highland barley sourdough, low-GI highland barley sourdough bread and a preparation method thereof. The highland barley sourdough is prepared by fermentation of Lactobacillus plantarum JYLP-326, highland barley flour and water. The low-GI highland barley sourdough bread prepared from the highland barley sourdough comprises the following components in parts by mass: 20-22 parts of highland barley flour, 22-28 parts of wheat flour, 36-40 parts of highland barley sourdough, 48-50 parts of water, 4-6 parts of bran, 4-6 parts of oat beta-glucan, 4-6 parts of inulin, 4-8 parts of gluten, 1-2 parts of yeast, 1-2 parts of salt and 4-6 parts of butter. The GI highland barley sourdough bread prepared by using the highland barley sourdough provided by the application has the advantages of low in-vitro glycemic index, low starch hydrolysis rate, high loft and softness, good taste and high appearance quality.
Owner:HEBEI AGRICULTURAL UNIV.

A method for directional regulation of starch-based edible film properties

The invention discloses a method for directional regulation of starch-based edible film performance, and belongs to the technical field of food packaging. The invention prepares edible film by enzymatically modifying starch, and uses starch branching enzyme to hydrolyze starch and transglycosylate, and regulates α, 1-4 and α, 1-6 glycosidic bonds in the starch molecular structure, thereby realizing the preparation of highly branched starch-based film. Through the transformation of enzyme action process conditions, the structural basis and molecular mechanism of highly branched starch film formation are explored, and the performance optimization and dynamic regulation of edible film are realized, which lays a foundation for the application of starch film in different food fields, and provides new ideas and new means for food environmentally friendly and degradable packaging and application.
Owner:JIANGNAN UNIV

A method for improving the secretion capacity of filamentous fungal proteins

ActiveCN116334121BHydrolysisAspergillus niger
The application discloses a method for improving the protein secretion capacity of Aspergillus and a recombinant strain obtained from the method. An16g07795 The method comprises performing genetic operation on Aspergillus to delete or reduce the activity of specific genes An16g07795 , so as to improve the protein secretion capacity of saccharifying enzyme, alpha-amylase, other proteins participating in starch hydrolysis and the like. The application utilizes the CRISPR-Cas9 genome editing technology to knock out the genes of Aspergillus niger, obtains a gene editing deletion mutant strain, and significantly improves the production level of starch hydrolysis enzymes, including the protein secretion level and the activity of saccharifying enzyme, so the application has great application value.
Owner:SHANDONG LONGKETE ENZYME PREPARATION +1

Enzymatic in situ production of modified alpha- 1,4-glucans and products obtained, enzymes and modified alpha- 1,4-glucans

The present disclosure relates, in part, to addition of glucosyl- or glucano-transferase type enzymes for producing and / or modifying alpha-glucan type structures in dough such as bread dough. Such enzymes encompass, for example, (i) dextrin dextranases, (ii) glucosyltransferases, and (iii) glucosyl-, maltosyl-, maltotetrasyl-, or glucano-transferases, where these enzymes can transfer glucose to / from starch and starch hydrolysate substrates (e.g., dextrin, amylodextrin, and / or maltodextrin) for production and / or modification of alpha-glucan type polymers in bread dough. Further disclosed are methods of preparing a baked food product by baking a farinaceous dough, comprising incorporating into the dough one or more of the foregoing enzymes – e.g., a glucosyl- or glucano-transferase type enzyme – wherein the enzyme can transfer a glucose-based moiety from a polysaccharide or oligosaccharide substrate to the non-reducing end of an oligosaccharide acceptor, thereby improving bread volume and / or dough stability. Further disclosed are modified alpha-glucans for use as food ingredients in general.
Owner:INT N&H DENMARK APS +1

Two malt hexosan amylase mutants with enhanced starch hydrolysis ability, and preparation method and application thereof

The application discloses two malt hexaose amylase mutants with enhanced starch hydrolysis ability, and a preparation method and application thereof. The amino acid sequences of the amylase mutants AmyM-A382T and AmyM-TR2 are shown in SEQ ID NO. 3 and SEQ ID NO. 4 respectively. The activity and conversion efficiency of the amylase mutant AmyM-A382T on raw starch and soluble starch are significantly improved. Compared with the amylase AmyM, the activity of AmyM-A382T is increased by 39% when soluble starch is used as a substrate, and the activity of AmyM-A382T is increased by 20-90% when raw starch of different sources is used as a substrate. The activity of the amylase mutant AmyM-TR2 on raw starch is significantly improved. Compared with the amylase AmyM, the activity of AmyM-TR2 is increased by 35-100% when raw starch of different sources is used as a substrate.
Owner:NANJING AGRICULTURAL UNIVERSITY

Raw starch hydrolase mutant AmyZ1: TG / S420W with improved specific enzyme activity and application of raw starch hydrolase mutant AmyZ1: TG / S420W

The invention discloses a raw amylolytic enzyme mutant AmyZ1: [delta] TG / S420W with improved specific enzyme activity and an application of the raw amylolytic enzyme mutant AmyZ1: [delta] TG / S420W. According to the invention, an alpha-amylase AmyZ1 variant AmyZ1: [delta] TG from Pontibacillus sp.ZY is used as a starting enzyme, and on the basis, a substrate binding site on the surface of the AmyZ1: [delta] TG is speculated through sequence alignment and structure alignment; the mutant AmyZ1: deltaTG / S420W for improving the rice raw amylase activity is obtained by subsequently exploring the distribution of surface substrate binding sites and the influence of amino acid types and number on alpha-amylase substrate preference. When the mutant takes raw rice starch as a substrate, the specific enzyme activity of the mutant is 1.33 times that of a starting enzyme, and the stability of the mutant is equivalent to that of the starting enzyme. The mutant has potential application value in the aspect of producing starch sugar by taking raw rice starch as a substrate.
Owner:ANHUI UNIV

Alpha-amylase variant

Provided is an α-amylase which has excellent amylolytic activity at a low temperature and further has excellent stability at a low pH and / or in the presence of a chelating agent. A variant of a parent α-amylase, comprising an amino acid residue substitution at a position corresponding to position H238 or E185 of an amino acid sequence set forth in SEQ ID NO: 2, and an amino acid residue substitution shown in the following (A) or (B), the parent α-amylase or α-amylase variant having at least 80% sequence identity to the amino acid sequence set forth in SEQ ID NO: 2 (excluding α-amylase variants having amino acid residue substitutions at three positions corresponding to positions H238, S239, and G178, three positions corresponding to positions H238, R209, and G178 and three positions corresponding to positions E185, N190, and G178 of the same amino acid sequence): (A) an amino acid residue substitution at one or more positions selected from the group consisting of positions corresponding to positions T116, A181, A199, A275, V277, A286 and L323 of the amino acid sequence set forth in SEQ ID NO: 2; or (B) an amino acid residue substitution at a position corresponding to position G178 of the amino acid sequence set forth in SEQ ID NO: 2 and one or more amino acid residue substitutions at one or more positions selected from the group consisting of positions corresponding to positions N126, T129, N190, R209 and S239 of the same amino acid sequence.
Owner:KAO CORP

Maojian tea cake with low glycemic index and preparation method of Maojian tea cake

The invention provides a tippy tea cake with a low glycemic index and a preparation method of the tippy tea cake, and belongs to the technical field of food processing. According to the invention, the oat, the tartary buckwheat, the highland barley and other coarse cereals, the dietary fiber powder and the corn starch are used for inhibiting the enzyme activity of amylase and glucose hydrolysis enzymes, reducing the hydrolysis rate of starch and delaying the rising rate of postprandial blood sugar; fructo-oligosaccharide and maltitol replace traditional white granulated sugar, the sucrose content of the tea cake is reduced, and the tea cake is healthier; maojian tea powder is used to enrich aroma of the tea cake and improve nutritional value of the tea cake, and the tea cake has corresponding functional health-care effects on human bodies. The low glycemic index tippy tea cake is prepared by controlling the total amount of coarse cereals such as oat, tartary buckwheat, highland barley and the like in a proper range and adopting multiple times of coloring, setting a proper wrapper and stuffing ratio and a baking process, so that the quality and the taste of the tea cake can reach the optimal state while the nutritional value and the hypoglycemic effect are considered, the wrapper is crisp, the stuffing is fragrant, and the tea cake is crisp, sweet and not greasy. And the preparation method is simple, easy to operate and suitable for large-scale production.
Owner:河南省农业科学院农产品加工研究中心 +1

Novel alpha-amylase for specifically hydrolyzing gelatinized starch and application

The invention provides a novel alpha-amylase for specifically hydrolyzing gelatinized starch. The amino acid sequence of the novel alpha-amylase is shown as SEQ ID NO.2 or SEQ ID NO.3. The invention also provides a preparation method of the novel alpha-amylase. The invention also provides a coding gene of the alpha-amylase as well as a recombinant expression vector and recombinant engineering bacteria containing the gene. Meanwhile, the invention also provides application of the novel alpha-amylase in preparation of dextrin with a low DE value. According to the invention, RoseTTAFold software is used for modeling wild type alpha-amylase, the structure of the wild type alpha-amylase is analyzed, and finally mutants S263A and I236R / S263A with better starch hydrolysis and gelatinization effects are screened by combining a mutation technology; the two optimized alpha-amylase mutants are used as novel alpha-amylase, not only can specifically hydrolyze gelatinized starch, but also have a better hydrolysis effect compared with a wild type.
Owner:ANHUI UNIV

Raw starch hydrolase mutant with improved stability and application thereof

ActiveCN119242618AIncreased specific enzyme activityBacteriaMicroorganism based processesHeterologousComputer Aided Design
The invention discloses a raw starch hydrolase mutant with improved stability and application thereof. According to the invention, alpha-amylase AmyZ2 is taken as a starting enzyme, and on the basis, computer-aided design is carried out, mutation sites are clear, heterologous expression is carried out in bacillus subtilis, and alpha-amylase with improved stability is obtained. When raw corn starch is used as a substrate, the specific enzyme activity of the mutant is 1.57 times that of a starting enzyme, and the stability of the mutant is 2 times that of the starting enzyme. Compared with the starting enzyme, the thermal stability of the mutant enzyme is greatly improved while the specific enzyme activity is maintained. The mutant has potential application value in starch sugar production with corn raw starch as a substrate.
Owner:ANHUI UNIV

Low glycemic index elaeagnus angustifolia naan and preparation method thereof

The invention discloses a low-glycemic-index elaeagnus angustifolia naan and a preparation method thereof, and aims to solve the problems that a traditional elaeagnus angustifolia naan is high in glycemic index and is not suitable for people who control sugar and lose weight to eat. The method comprises the following steps: 1) preparing elaeagnus angustifolia dietary fiber powder, performing enzymolysis on elaeagnus angustifolia powder step by step through alpha-amylase, alkaline protease and saccharifying enzyme, and combining ultrasonic assistance and centrifugal separation to obtain high-purity dietary fibers; and (2) mixing the wheat flour, the elaeagnus angustifolia dietary fiber powder and the elaeagnus angustifolia fruit powder according to a mass part ratio of (85-90): (5-10): 5, performing dough kneading, performing fermentation twice, making crusty pancake blanks, and performing segmented temperature control baking. Experiments show that the starch hydrolysis rate of the prepared elaeagnus angustifolia naan is remarkably reduced, the glycemic index can be effectively reduced, the elaeagnus angustifolia naan is suitable for people who need to control blood sugar and lose weight, and meanwhile the flavor and nutrition of elaeagnus angustifolia are reserved.
Owner:TARIM UNIV

Powdered composition for cosmetic cleansing of skin or hair

Powder compositions, which contain, in addition to at least one surfactant, a specific mixture of thickeners of starches or starch fractions possibly modified in a hydrophobic manner, of starch hydrolysis products and at least one natural polymer other than i) and ii), suitable for the rapid and simple provision of a ready-to-use, cosmetic, fluid cleaning agent with good cleaning and care potential.
Owner:HENKEL KGAA

Aspergillus chitin hydrolase, coding gene and application thereof

The invention discloses aspergillus chitin hydrolase, a coding gene and application thereof. The amino acid sequence of the gene is shown as SEQ ID NO. 1 or SEQ ID NO. 2. The aspergillus is subjected to genetic manipulation to increase or enhance the activity of a specific gene, so that the secretion ability of glucoamylase and other proteins participating in starch hydrolysis can be improved. In a specific experiment, recombinant strains with high-level expression of genes are respectively obtained by overexpressing aspergillus niger genes AnNagA and AnCfcA, the recombinant strains can obviously improve the production level of amylolytic enzyme, the protein secretion level is respectively improved by about 22% and 18% compared with that of an original strain, the activity of saccharifying enzyme is improved by about 27% and 23% compared with that of the original strain, and the yield of the amylolytic enzyme is improved by about 30% and 25% compared with that of the original strain. And the method has a relatively high application value.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Quick-frozen sharpleaf galangal fruit braised noodles with low glycemic index and preparation method thereof

The invention provides quick-frozen sharpleaf galangal fruit braised noodles with low glycemic index and a preparation method thereof, and belongs to the technical field of food processing. The braised noodles comprise the following raw materials in parts by weight: 30-60 parts of whole wheat flour, 5-20 parts of resistant starch, 5-20 parts of oat flour, 5-20 parts of highland barley flour, 5-10 parts of tartary buckwheat flour, 10-20 parts of wheat dietary fiber powder and 1-3 parts of a fructus alpiniae oxyphyllae extract. By using the self-made coarse cereal powder and the fructus alpiniae oxyphyllae extract, the enzyme activity of amylase and glucose hydrolysis enzymes can be inhibited, the starch hydrolysis rate is reduced, the postprandial blood sugar rising rate is delayed by using the resistant starch, and the dosage of the coarse cereals such as the oat, the tartary buckwheat and the highland barley in the total proportion is controlled within a proper range; and the noodles can be ensured to have a relatively good taste while the nutritional value and the blood sugar reducing effect are considered. The taste and flavor of the braised noodles after quick-freezing and reheating are improved by optimizing the formula and process of the braised noodles, and finally the braised noodle product with convenience and health is prepared.
Owner:河南省农业科学院农产品加工研究中心 +1

Preparation method of purslane extracting solution with soothing effect

The invention relates to a preparation method of a purslane extracting solution with a soothing effect, and belongs to the field of cosmetics and biological extraction. The preparation method comprises the following steps: crushing fresh wild purslane, adding a butanediol-water mixed solvent, carrying out ultrasonic wall breaking treatment at 55-58 DEG C, and adding plant fiber hydrolase, vegetable gum hydrolase, plant starch hydrolase and plant protein hydrolase to carry out synergistic enzymolysis extraction; heating, inactivating and filtering the extracting solution, sequentially performing multi-stage purification by adopting chitosan flocculation, activated carbon decoloration, ceramic membrane filtration, ultrafiltration and nanofiltration, and collecting trapped fluid with the molecular weight of more than 150-300 Da through nanofiltration; and adding the humectant and the preservative. Through combination of ultrasonic-enzymolysis synergistic extraction and multi-stage membrane separation and purification, active ingredients such as polysaccharide and flavone in purslane are efficiently reserved under mild conditions, the inhibition rate of the obtained extracting solution to hyaluronidase activity reaches 20% or above, and the extracting solution has a soothing effect and good product stability and can be applied to soothing cosmetics or skin care products.
Owner:FOSHAN JIEGUAN BIOTECHNOLOGY CO LTD

A method for simultaneously improving starch hydrolysis rate and protein quality in hot-pressed peanut meal

ActiveCN116784423BIncrease the degree of hydrolysisIncreased reducing sugar contentFood processingAnimal feeding stuffBiotechnologyEnzymatic hydrolysis
The present invention belongs to the technical field of deep processing of peanuts, and specifically relates to a method and application thereof for simultaneously improving the starch hydrolysis rate and protein quality in hot-pressed peanut meal. The specific method is that the peanut meal is pretreated first: water is added after ultrafine grinding, the pH is adjusted to 2, and constant temperature extraction is performed; after extraction, the pH is adjusted to 6, high temperature α-amylase is added, constant temperature oscillation is performed for enzymolysis, the pH is adjusted to 4.2-4.7, the enzyme is inactivated, and centrifugation is performed; protein extraction: water is added to the precipitate after centrifugation, the pH is adjusted to 8-10, and the precipitate is obtained by centrifugation; the precipitate is repeatedly extracted once, the supernatant is combined and the pH value is adjusted, and the precipitate is collected by centrifugation again; it is washed with deionized water, the pH is adjusted to 7.0-7.2, and the peanut meal protein is dried to obtain. The method of the present invention combines pretreatment with enzyme treatment technology; on the one hand, it can improve the degree of starch hydrolysis and increase the content of reducing sugars; on the other hand, the protein purity, emulsification, emulsification stability and the content of amino acids of peanut meal protein are improved; and the added value of the product is greatly improved.
Owner:SHANDONG ACADEMY OF AGRICULTURAL SCIENCES

Composite bio-enzyme preparation for treating polluted water and preparation method of composite bio-enzyme preparation

The invention relates to the technical field of pollution treatment, and particularly discloses a composite bio-enzyme preparation for polluted water treatment and a preparation method of the composite bio-enzyme preparation. The invention relates to a composite biological enzyme preparation for polluted water treatment. The composite biological enzyme preparation comprises the following raw materials in parts by weight: 25-30 parts of an amylolytic enzyme compound, 20-25 parts of a proteolytic enzyme compound, 15-20 parts of lipase, 10-15 parts of cellulose hydrolase, 15-20 parts of flora freeze-dried powder, 10-15 parts of porous calcium alginate microspheres, 5-8 parts of chitosan modified silicon dioxide and 3-5 parts of an enzyme stabilizer. According to the application, the content of total microcystic toxins and the content of refractory organic matters in the polluted water can be obviously reduced, the sewage treatment effect is facilitated, and secondary pollution in the sewage treatment process is reduced.
Owner:JIANGSU JIXU TECHNOLOGY IND GROUP CO LTD

Multi-stage temperature control lactic acid biological fermentation process method for corn and soybean compound substrate

The invention discloses a multi-stage temperature control lactic acid biological fermentation process method for a corn and soybean compound substrate. A high-temperature gelatinized corn and soybean composite substrate is used as a raw material, starch hydrolysis fermentation is performed in stages under the action of high-temperature amylase, glucoamylase, pullulanase and bacillus coagulans, and the three enzymes are fixed on a special magnetic nano-carrier through covalent bonds. The method disclosed by the invention has the beneficial effects that 1, a composite hydrolysis system is formed by adopting low-dose high-temperature amylase, glucamylase and pullulanase, and more than 80% of starch can be converted into glucose by virtue of an enzyme activity synergistic effect through stepped temperature regulation and control; 2, by generating a high-concentration glucose substrate, metabolic inhibition of a low-sugar environment on bacillus coagulans in a traditional step-by-step process is avoided, and the conversion rate of converting glucose into lactic acid exceeds 50%; 3, the enzymolysis-fermentation process is seamlessly connected, and the middle links of inactivation, centrifugation and the like in the traditional process are avoided; and 4, the enzyme can be repeatedly used, and the cost is reduced.
Owner:DALIAN UNIV

A raw starch hydrolyzing enzyme mutant, expression strain and application thereof

The application discloses a raw starch hydrolysis enzyme mutant, an expression strain thereof and application. Pontibacillus The alpha-amylase AmyZ1 of sp.ZY is used as a starting enzyme, a substrate binding sub-site is determined through molecular docking, a site-directed mutation is performed on a residue at a +1 site 199, and a mutant enzyme L199F with higher glucose yield is obtained. When the mutant enzyme is used in an industrial sugar production process and corn raw starch is used as a substrate, the DE value of the corn raw starch after liquefaction for 2 hours can reach 35%, which is 1.44 times that of the starting enzyme; the DE value can reach 99% after saccharification for 18 hours, which is 5 hours earlier than that of the starting enzyme. Therefore, the mutant enzyme has high potential application value in the field of corn raw starch-based sugar production industry.
Owner:ANHUI UNIV

Black truffle heptapeptide for inhibiting starch digestion and application of black truffle heptapeptide

PendingCN121494926APeptidesFood ingredient functionsBiotechnologyDigestible starch
The invention relates to the technical field of development of active peptides, in particular to a novel small-molecule heptapeptide with a starch digestion inhibiting effect and application thereof. According to the method, perigord truffle is taken as a raw material, and the perigord truffle polypeptide is obtained through protein extraction, protein enzymolysis, dialysis and freeze-drying. Furthermore, a bioinformatics tool is used for predicting the activity, potential toxicity, solubility and physicochemical properties of the peptide fragment, wheat starch is selected as a test material, the digestion characteristic of the polypeptide-starch compound is detected, and the peptide fragment is determined according to the starch hydrolysis rate and the ratio of rapidly digested starch to slowly digested starch to resistant starch. The heptapeptide AADELFR with the effect of inhibiting or delaying starch digestion is obtained and is applied to preparation of food or health food beneficial to maintaining the healthy level of blood sugar.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Production of clean starch hydrolysates

Described is a process for producing high quality starch hydrolysates using course-ground grains and seeds in a high temperature steeping process. The process is ideal for producing high quality starch hydrolysates in an energy-efficient manner, as well as for producing high quality animal feed and oil co-products.
Owner:DANISCO US INC

Processing technology of maltodextrin with low DE value

The invention discloses a processing technology of low-DE-value maltodextrin, belongs to the technical field of maltodextrin, and aims to solve the problems of insufficient hydrolysis end point control precision and poor product batch stability when the low-DE-value maltodextrin is prepared by the existing technology. The process comprises the following steps: humidifying starch until the water content is 18-22%, carrying out instantaneous treatment for 15-30 seconds by using saturated steam at 105-110 DEG C to form a surface gelatinization layer, dispersing into 20-30% suspension, carrying out pulsed electric field treatment under the conditions of 25-35 kV / cm electric field intensity and 80-150 pulse counts, carrying out enzymolysis by using medium-temperature alpha-amylase, inactivating enzyme, filtering, concentrating and drying to obtain the product. According to the technology, starch is pretreated through cooperation of saturated steam instantaneous surface gelatinization and pulsed electric field treatment, then enzymolysis is conducted through medium-temperature alpha-amylase, the starch surface and interior hydrolysis rates are effectively regulated and controlled to tend to be synchronous, and therefore the batch stability of low-DE-value products is remarkably improved.
Owner:ZHAOQING HUANFA BIOTECHNOLOGY CO LTD

Methods for sugar regulation

The present invention relates to a method for preparing a food product comprising hydrolyzed starch, and to products obtainable by the method. Compared to conventional methods of starch hydrolysis, the method has the advantage of reducing the amount of sugars (i.e. maltose) produced by the hydrolysis, and presents the additional advantage of providing good processability for the food product.
Owner:SOCIETE DES PRODUITS NESTLE SA