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193 results about "Starch hydrolysis" patented technology

Starch Hydrolysis Starch agar is a differential medium that tests the ability of an organism to produce certain exoenzymes, including a-amylase and oligo-1,6-glucosidase, that hydrolyze starch.

Preparation method of bacillus subtilis HJDA32 and bacteriocin generated by bacillus subtilis HJDA32

A bacillus subtilis HJDA32 strain is preserved on September 25, 2012 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6624, which is separated from solid fermentative substrate of vinegar in the Shanxi province Qingxu mature vinegar plant. The strains are cultured in a beef extract peptone basic medium and arranged in pole, single, paired or chained shape with the size between 0.6-0.85mu m*1.5-3.5mu m, and have capsules; elliptical spores are cultured on a raw spore culture medium; the strain is identified to be bacillus subtilis through catalase and carbon source utilization test, gelatin hydrolysis, starch hydrolysis, ethanol oxidation, acetic acid oxidation and other physiological and biochemical identifications and 16SrDNA (deoxyribonucleic acid) phylogenetic analysis; and the bacillus subtilis HJDA32 strain is used as a production strain, cultured with fermenting culture fluid, and subjected to centrifuge, ammonium sulfate powder salting out and centrifuge to obtain the bacteriocin. The bacteriocin is wide in antimicrobial spectrum and high in bacteriostatic activity, can inhibit various food-borne pathogenic bacteria, as well as gram positive and gram negative bacteria causing food spoilage, and can be used in food preservatives.
Owner:SHANXI AGRI UNIV

Production process of instant maize germ powder

The invention relates to a production process of instant maize germ powder. The instant maize germ powder is prepared through raw material preprocessing, beating, enzymolysis, enzyme inactivation, colloid milling, sterilization, high-pressure homogenization and spray drying by taking maize germ meal as a raw material; the enzymolysis is carried out by adding medium-temperature alpha-amylase accounting for 0.2%-0.4% of the dry weight of the maize germ meal, and the starch hydrolysis DE value (Dextrose Equivalent value) of maize germ meal slurry which is subjected to enzymolysis is controlled between 18% and 22%; the prepared homogenized maize germ meal slurry achieves the easiness for the spray drying, and a finished product does not absorb moisture, so that the dissolution stability of the maize germ powder is enhanced, the deposition has small possibility of appearing in the dissolving process, and the maize germ powder is not caked and agglomerated when being dissolved in water; and the additional value of maize germ meal is increased. The instant maize germ powder obtained through the method disclosed by the invention achieves the protein content more than 23%, is in a faint yellow color and is fast in dissolution; and the dissolved instant maize germ powder is uniform in state and achieves the product dissolution time less than 27 seconds without any unpleasant odor.
Owner:鲁洲生物科技(辽宁)有限公司 +2

Porous starch and joint preparation method for liquid glucose used for fermentation thereof

The invention relates to porous starch and a joint preparation method for liquid glucose used for fermentation of the porous starch. The joint preparation method comprises the steps of size mixing 1000 parts by dry weight of starch raw materials with water, regulating the concentration of starch milk to be 40%-50% and the potential of hydrogen (pH) of 4.0-6.0, under condition of the temperature of 30 DEG C-55 DEG C, adding 0.5-5.0 parts by weight of amylase, stirring for 5-30 hours for enzymolysis, exhausting the liquid, washing, drying, smashing and obtaining solid powdery porous starch, utilizing enzymolytic liquid glucose and washing liquid in the process of preparation of the porous starch for size mixing of the starch, liquefying, saccharifying, transforming protein, filtering, concentrating and carrying out other procedures. According to the joint preparation method, enzymolysis of glucose amylase or composite saccharifying enzyme is utilized for preparation of the porous starch, the sugar component of starch hydrolysate is mainly glucose, and products with glucose as main composition are generated. If beta-amylase or fungus amylase is utilized, or the beta-amylase or fungus amylase is carried out saccharification with debranching enzyme, products with maltose as main composition are generated.
Owner:SOUTH CHINA UNIV OF TECH +1

Bacillus megaterium strain and application thereof

The invention discloses a bacillus megaterium strain (bacillus megaterium L2) and anapplication thereof. The bacillus megaterium strain has the morphological characteristics that the strains are cultivated on an ordinary agar culture medium for 36h at the temperature of 37 DEG C, a shape of a colony is similar to a circle, the surface of the colony is salient, yellow and not transparent, the colony is 2-4mm, the edge of the colony is tidy, and the surface of the colony is glossy or darker and does not secrete pigments; the strains are gram-positive bacteria, are in a shape of a rod, are (1.2-1.5)*(2.0-4.0) microns, are arranged in a chain shape and have the motility, and a spore is in an ellipse shape, is terminal or subterminal and is not obvious in expansion; and the strains can endure cultivation by 7 percent of NaCl, are positive to calalase, glucose ferment, amylolydrolysis, nitrate reduction, gelatin liquefaction, semisolid puncturing test, milk decomposition, citrate utilizing test and pH 5.7, and are negative to VP (Voges-Proskauer) test, benzpyrole test and mannitol hydrolysis. According to the bacillus megaterium strain disclosed by the invention, a heat-resistant spore can be produced, and the bacillus megaterium strain is easy to produce and process in dosage form and is also beneficial to survival, colonization and propagation.
Owner:BIJIE COMPANY OF GUIZHOU TOBACCO +1

New bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, andacquiring method and application of same

The embodiment in the invention provides a new bacterial strain degrading polycyclic aromatic hydrocarbons with five rings or six rings, and an acquiring method and application of the same, relates to the technical field of biological processing for environment pollutants, and the new bacterial strain is capable of efficiently degrading the polycyclic aromatic hydrocarbons with five rings or six rings. The new bacterial strain has the name of ZH-L8, the morphological characteristics comprise that a circle milk-white bacterium colony is grown on a solid medium and has a smooth edge; the new bacterial strain is in the shape of a rod when being observed under a microscope; the cell shows a Gram-positive rod shape, the later sides are parallel, and two ends are blunt; and the bacterium colony is circle, the edge is smooth, the bacterium colony is tightly adhered to the medium, and the bacterium colony is white and not transparent. The physiological biochemical characteristics comprise that the bacterium well grows in the temperature scope of 20-45 DEG C, grows well when pH is 5.7 and 6.8, has the salt-resistant concentration of 5%, and is methyl-red positive, contact-enzyme positive, v-p negative with the v-p solution final pH less than 6, arginine utilization positive, nitrate utilization positive, positive for D-glucose acid production, lactose acid production and cane sugar acid production, positive for starch hydrolysis, and the like.
Owner:HEBEI AGRICULTURAL UNIV.
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