6 results about "Amylosucrase activity" patented technology

The present invention relates to isolated polypeptides having alpha-amylase activity, polypeptides comprising an alpha-amylase catalytic domain and a carbohydrate binding module, and polynucleotides encoding the polypeptides, compositions comprising said polypeptides, as well as polynucleotides and nucleic acid constructs or vectors encoding the polypeptides, and host cells comprising the polynucleotides or constructs / vectors, as well as methods of producing and using the polypeptides.

The application discloses application of calyculostero in preparation of blood sugar reducing drugs, and belongs to the technical field of pharmacological action of calyculostero. Experimental results show that the calyculostero can inhibit the activity of pancreatic alpha-amylase (IC50 is 4.34+ / -1.10 ug / mL) in a dose-dependent manner, reduce postprandial blood sugar, and improve sugar tolerance, reduce fasting blood sugar, improve total cholesterol and triglyceride levels, and reduce histopathological changes of liver, pancreas and kidney in long-term intervention, and has no obvious hepatotoxicity. The calyculostero can be used alone or together with a pharmaceutically acceptable excipient to prepare a pharmaceutical composition, and is used for reducing blood sugar or treating diabetes, and the dosage form includes oral preparations, injections and the like. The application provides a new natural medicine selection for drug treatment of diabetes.

The present application relates to a kind of zanthoxylum bungeanum essential oil emulsion starch enzyme activity detection method, which is by using centrifugal demulsification method combined with accurate chemical analysis method, the starch enzyme activity in zanthoxylum bungeanum essential oil emulsion is quantitatively analyzed, specifically including the following steps: S1, instrument and reagent preparation;S2, solution preparation;S3, sample processing;S4, demulsification treatment and DNS method detects absorbance and calculates starch enzyme activity;The present application can accurately quantify the reducing sugar level in essential oil emulsion, the accuracy compared with direct detection method is significantly improved, and the method is suitable for the starch enzyme activity detection in other essential oil emulsion.

This invention belongs to the field of functional composition technology, and discloses a weight-loss and body-shaping composition, its preparation method, and its application. The composition, by mass percentage, comprises the following ingredients: 21-25% green coffee, 17-19% white kidney bean extract, 12-18% collagen peptides, 17-25% inactivated compound probiotic powder, and 23-25% mixed fruit and vegetable powder. Green coffee releases the active ingredient chlorogenic acid; white kidney bean extract inhibits α-amylase activity; collagen peptides are easily absorbed and stimulate satiety-related hormones; and the inactivated compound probiotic powder acts as a post-biotic, regulating the intestinal flora environment. This invention achieves its effects through the combined action of three pathways: enhancing satiety, reducing absorption, and regulating metabolism. It can prevent excessive calorie intake, reduce hunger, and simultaneously regulate metabolism. It also increases energy consumption and fat mobilization, provides defecation motivation, regulates intestinal flora balance, and is natural, gentle, and does not harm the human body.

The present invention provides a detergent composition containing α-amylase that exhibits high specific activity at low temperatures. [Solution] A detergent composition containing one or more proteins selected from (A), (B), (C), and (D) below. (A) A protein having an amino acid sequence that is at least 90% identical to the amino acid sequence shown in Sequence ID No. 2, and which also has α-amylase activity. (B) A protein having an amino acid sequence that is at least 90% identical to the amino acid sequence shown in Sequence ID No. 4, and which also has α-amylase activity. (C) A protein having an amino acid sequence that is at least 90% identical to the amino acid sequence shown in Sequence ID No. 6, and which also has α-amylase activity. (D) A protein having an amino acid sequence that is at least 90% identical to the amino acid sequence shown in Sequence ID No. 8, and which also has α-amylase activity.

The present application belongs to the technical field of extraction of natural bioactive substances, and particularly relates to a method for extracting flavonoids from hawthorn leaves. The method comprises the following steps: mixing hawthorn leaves with a hydrophobic eutectic solvent, performing ultrasonic treatment, and then centrifuging to obtain an extraction solution. The total flavonoid content extracted by the method is 3.69 times that of ethyl acetate extraction solution and 6.11 times that of ethanol extraction solution. The average DPPH free radical scavenging rate of the extracted extract is 74.35% of the same concentration of vitamin C, the alpha-glucosidase activity inhibition rate is 1.24 times the inhibition rate of the same concentration of acarbose, and the alpha-amylase activity inhibition rate is 2.80 times the inhibition rate of the same concentration of acarbose.