172 results about "Amylosucrase activity" patented technology

The present invention relates to a process for making a bread product. The process includes the addition of a non-maltogenic exoamylase that hydrolyses starch to a starch medium, and the application of heat to the starch medium. The non-maltogenic exomylase cleaves one or more linear malto-oligosaccharides, predominantly consisting of from four to eight D-glucopyranosyl units, from non-reducing ends of amylopectin side chains. The non-maltogenic exoamylase has an endoamylase activity of less than 0.5 endoamylase units (EAU) per unit of exoamylase activity.

Disclosed are fermented soybean protein products with enhanced enzyme productivity and methods for producing them by solid fermentation using Bacillus subtilis GR-101 and additionally Aspergillus oryzae GB-107 (KCTC 10258BP). A novel Bacillus subtilis GR-101 strain (KCCM 10673P), which has excellent protease productivity in soybean meal, is also disclosed. Fermentation using Bacillus subtilis GR-101 strain (KCCM 10673P) producing protease combined with Aspergillus oryzae GB-107 (KCTC 10258BP) having amylase activity can remove trypsin inhibitors known as anti-nutritional factors and thus can provide soybean peptides with high digestion ratio and excellent enzyme activities. Feed comprising the soybean peptides as active component and capable of improving productivity of livestock and food for human comprising the soybean peptides supply is further disclosed.

The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

The invention discloses a dried chemical indicator paper to test human alpha-diastatic activity in the external clinical diagnostic agent technical domain, which is composed of long-bar shaped upper and lower support layers, each middle testing layers, wherein the indicator paper is divided into hand-held area and testing area, wherein the testing area is dispersing layer, filter layer and agent layer from upwards to downwards; the dispersing layer is selected from kinds of grid material of synthetic fiber material; the filter layer can be kinds of filter material to filter blood; the agent layer is asymmetrical synthetic film; one sampling hole is opened on the upper support layer for sample to enter the dispersing layer to permeate the filter layer and agent layer and change color at agent layer due to chemical reaction; the testing hole of lower support layer can test the change of optical density in the reacting course. The inveniton can test the human alpha-diastatic activity quantitatively, which provides evidence for acute pancreatitis.

The invention belongs to the technical field of recombinant fusion protein medicaments. In the method, capsid protein genes cotC of spores of bacillus subtilis are used as molecular vectors and are recombined with human serum albumin hsa genes, so that integrated recombinant plasmid for fusion expression is constructed; after the bacillus subtilis is transformed through the recombinant plasmid for fusion expression of the human serum albumin HAS, exogenous genes are integrated in amylase genes amyE at specific sites on chromosomes through homologous recombination, and the amylase genes are subjected to insertional inactivation, so that a recombinant strain has no amylase activity, the recombinant bacillus subtilis strain comprising fusion expression CotC-HAS is successfully screened, and the human serum albumin is displayed on the surface of the spore produced by inducing the recombinant strain. The recombinant spore can be directly and orally taken by animals; and the recombinant spore has a part of medicinal effects and nutrition functions of the human serum albumin on the basis of keeping physiological property of the spore.

The invention belongs to the biotechnical field, especially relates to a pentapeptide with an auxiliary hyperglycemic function, and concretely relates to a pentapeptide KLPGF capable of inhibiting the activity of alpha-glucosidase and inhibiting the activity of alpha-amylase. The amino acid sequence of the pentapeptide KLPGF is Lys-Leu-Pro-Gly-Phe. The pentapeptide can be used to develop auxiliary hpyerglycemic drugs and functional foods.

The invention provides Aspergillus oryzae ZA156 suitable for producing soy sauce, and relates to an application of Aspergillus oryzae for increasing original glutamic acid of the soy sauce. The Aspergillus oryzae ZA156 neutral protease activity and amylase activity are high, glutaminase vitality is high, the Aspergillus oryzae ZA156 is suitable for producing original high-freshness soy sauce, theglutamic acid in raw oil is increased by about 10%, the amino nitrogen yield is increased by 8%, the deposition amount is reduced by 3%, and the appearance and delicious taste of the soy sauce throughbrewing and fermentation are obviously increased.

The invention provides a new use of a macrocyclic polyphenol compound separated from a penthorum chinense pursh extract in pharmacy. The macrocyclic polyphenol compound comprises a compound 1, a compound 2, a compound 3 and a compound 4, wherein the structural formulas of the compounds are as shown in the specification; the compounds have the following pharmacological activities that (1) the alpha-amylase activity is significantly inhibited, and the compounds have certain hypoglycemic effects, and (2) TGF (transforming growth factor)-beta1-induced hepatic stellate cell proliferation is significantly inhibited, and the compounds have anti-liver-fibrosis effects by pharmacology and efficacy experiments. The invention provides a novel medicament for preventing and treating diabetes mellitus, and an anti-liver fibrosis medicament. The medicament is abundant in raw material source, small in side effect, and beneficial to environmental protection, and has a relatively great clinical application value.

The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with corn based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for at least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feedstuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.

A high temperature alpha-amylase and a high efficiency preparation method of the mutant thereof belongs to the fermentation engineering field. The invention provides a method for high efficiently secreting expressing a high temperature alpha-amylase and mutant thereof with alpha-amylase activity through a bacillus, especially a bacillus licheniformis mutant strain as a host cell. The synthesis and secretion level of the high temperature alpha-amylase or mutant thereof of the recombinant strain of the invention reaches 18-25mg/mL during fed-batch fermentation and is 3-4.5 times of the original strain high temperature alpha-amylase synthesis level. The high temperature alpha-amylase of the invention is useful for reducing the fermentation manufacture cost of the industrial enzyme, simplifying the fermentation manufacture process and reducing fermentation industrial environment pressure.

The invention relates to a disease-resistant microbial preparation which is special for broiler chickens, wherein, the strain is bacillus subtilis, a liquid preparation is obtained by means of submerged fermentation, a solid preparation is obtained by means of carrier adsorption or solid fermentation. The microorganism have significant inhibitory effect on escherichia coli in vitro, and have strong therapeutic effect on respiratory diseases, white diarrhea, yellow diarrhea and other diseases of the broiler chickens, simultaneously, the microorganism have high activity of protease, lipase and amylase, and have strong degradation ability for botanical carbohydrate. The disease-resistant microbial preparation has the advantages of improving feed conversion rate, increasing the appetite, promoting growth and enhancing disease resistance, and can be used in stages for the broilers growing poorly and sick broiler chickens.

The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature.

The present invention relates to an α-amylase activity inhibitor, and to food and beverages comprising the α-amylase activity inhibitor. The α-amylase activity inhibitor of the present invention exhibits remarkably excellent α-amylase inhibitory activity as compared with guava extract. Accordingly, food and beverages containing the α-amylase activity inhibitor are diet food and beverages suitable for people of high blood-sugar level or hyderlipidemia.

The invention discloses a preparing method of thermophilic alpha-amylase (PFA), which comprises the following steps: heating and dissolving recombinant protein inclusion body under the condition of alkalinity; diluting; compositing; separating and purifying through column layer. This method possesses high reclaiming ratio and high alpha-amylase activity.

The present invention relates to polypeptides having alpha-amylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.