74 results about "Amylosucrase activity" patented technology

The invention belongs to the field of food microorganisms, and particularly relates to a saccharomycopsis fibuligera strain, a microbial inoculum, a fermented product and preparation and application thereof. The saccharomycopsis fibuligera strain provided by the invention is preserved in the China Center for Type Culture Collection on September 25, 2023, and the preservation number of the saccharomycopsis fibuligera strain is CCTCC NO: M 20231788. The saccharomycopsis fibuligera strain provided by the invention can produce amylase, protease, cellulase and other enzymes required by fermentation, the amylase has the activity of pullulanase and raw amylase and can well adapt to the special environmental conditions of fermentation, and the saccharomycopsis fibuligera strain has high environmental tolerance and high aroma-producing capacity; therefore, the quality of fermented products is improved, and the fermentation production investment cost is reduced.

Described herein are genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

The invention discloses an application of astaxanthin in preparation of a regulator for improving the gentisic acid level. On the basis of non-targeted metabonomics analysis, it is found for the first time that in the SAP pathogenesis process, the level of a flora metabolite, namely gentisic acid (GA), in intestinal contents is remarkably reduced, and in-vivo gentisic acid level can be remarkably callback through astaxanthin intervention. Further research proves that exogenous supplement of gentisic acid can effectively simulate the protection effect of astaxanthin, including improvement of pancreatic tissue pathological damage, reduction of serum lipase and amylase activity, and inhibition of systemic inflammatory response (down-regulation of IL-1beta, IL-6 and TNF-alpha and up-regulation of IL-10). The invention not only discloses a new mechanism that astaxanthin plays a therapeutic role through an'intestinal flora-metabolite 'axis, but also determines that gentisic acid is used as a novel drug target for treating SAP, and constructs a new method for screening drugs for treating SAP according to the novel drug target.

The present application relates to a kind of metal-organic framework mediated multi-enzyme nanoparticles and its application, especially the method for alpha-amylase detection. By detecting product absorbance, the quantitative detection of alpha-amylase activity is realized. According to the nature of MFM analog enzyme and its ability as natural enzyme carrier, Agl@MFM@Gox@MFM is prepared, compared with natural enzyme, the composite material has strong resistance, can successfully protect the activity of enzyme. Based on the cascade catalytic system of multi-enzyme nanoparticles Agl@MFM@Gox@MFM, a biosensor is first synthesized and established for detecting alpha-amylase, successfully combines chemical catalyst with natural enzyme, has wide detection linear range (5-500 U / L) while has excellent specificity to alpha-amylase. Benefited from the advantages of low cost, easy preparation and excellent stability, the colorimetric sensor has been successfully used to determine the activity of alpha-amylase in koji.

The invention discloses a graded preparation method of rice flour capable of promoting nutrient absorption based on digestive function development of infants. According to the invention, high-liquification and high-saccharification type, medium-liquification and high-saccharification type and low-liquification and medium-saccharification type enzymolysis rice flour is simulated and optimized by adopting models with different gastric acid pH values, digestive enzyme systems and gastrointestinal motility rules for the first time. According to the method, three parameters, namely different rice flour raw materials, gelatinization and composite enzymolysis parameters and different granularities, are combined and regulated, so that a rice flour product which is highly matched with gastric acid environments, amylase activity and intestinal tolerance in different developmental stages of infants is constructed. According to the method disclosed by the invention, the absorption efficiency and the energy utilization effect of carbohydrates in the body of the infant are remarkably improved, the digestive dynamic characteristics of the infant are scientifically adapted, the digestion problem caused by mismatching of starch composition and intestinal functions is effectively avoided, the risks of abdominal distension, diarrhea and constipation are remarkably reduced, and efficient utilization and healthy metabolism of nutrients are guaranteed.

The invention discloses a functional solid beverage capable of reducing fat, losing weight and conditioning five highs. According to the functional solid beverage, dietary fibers such as fructo-oligosaccharide, inulin and the like are used for regulating intestinal flora, a white kidney bean extract is used for inhibiting the activity of alpha-amylase, L-carnitine is used for promoting fat transport, and medicinal and edible components such as ginseng, lotus leaves and the like are matched to realize multi-way metabolism regulation. The weight percentage of each component can be adjusted within a specified range so as to adapt to different physique requirements. Animal experiments prove that the total cholesterol (TC) of serum can be obviously reduced by 21.3-25.8% and the triglyceride (TG) can be obviously reduced by 28.9-34.2% after the traditional Chinese medicine is continuously taken for 4 weeks, and meanwhile, the insulin resistance index (HOMA-IR) can be improved to 16.5-20.3%.

The invention discloses a real-time detection method for bulbus fritillariae propagating activity, and relates to the field of seedling detection, and the method comprises the following steps: step 1, seed stratification pretreatment: mixing bulbus fritillariae seeds and fine sand according to a ratio of 1: 3 for stratification, and maintaining the humidity of a substrate to be 50-60%; 2, dynamically monitoring enzyme activity, namely grinding the sample prepared in the step 1, sampling and detecting the seeds every 10 days, extracting enzyme liquid, and respectively measuring the activity of catalase, peroxidase and amylase in real time by adopting an ultraviolet spectrophotometric method; a full-cycle real-time data flow is constructed, a continuous dynamic process of activity change is captured, then a machine learning model is used for fusion analysis and prediction of seed propagation potential, activity evaluation is converted into a quantifiable propagation activity index, a clear judgment threshold value is set, and the machine learning model is trained on the basis of a large amount of data, so that the propagation activity of seeds is improved. And the accuracy and efficiency of high-quality seed source screening are improved.

The invention relates to the field of protein, in particular to a milk-derived peptide APPSPIGVF with antihypertensive activity as well as a preparation method and application of the milk-derived peptide APPSPIGVF, and the amino acid sequence of the milk-derived peptide APPSPIGVF is Ala-Pro-Pro-Ser-Pro-Ile-Gly-Val-Phe. An angiotensin converting enzyme activity inhibition experiment, an alpha-amylase activity inhibition experiment and a dipeptidyl peptidase-4 activity inhibition experiment prove that the milk-derived peptide APPSPIGVF has very good functions of resisting hypertension and reducing blood sugar. The milk-derived peptide APPSPIGVF disclosed by the invention has an anti-hypertension capability, and can be used for remarkably inhibiting the activity of alpha-amylase and dipeptidyl peptidase-4, improving the capability of resisting chronic diseases such as hypertension and hyperglycemia of an organism, reducing the morbidity of the organism and improving the quality of life; the method has very important significance for developing food, health care products and medicines with the functions of resisting hypertension and reducing blood sugar.

The invention discloses a Chinese yam cake with a compound sour system for synergistically activating salivary amylase and a preparation method thereof. The Chinese yam cake comprises the following raw materials: Chinese yam, konjaku flour or oligosaccharide, a sweetening agent, a gelling agent, malic acid, citric acid, hawthorn, dark plum and water. The main structure matrix is jointly constructed by Chinese yam powder, konjaku flour / oligosaccharide and gels to form a cake body base; a composite sour system is uniformly distributed in a gel network, a complementary system is formed through superposition of various organic acids, and the composite sour system has good buffer capacity, can maintain weak acidic pH in the oral cavity and activate the activity of alpha-amylase without inhibition, and is different from the problem that a single acid system is prone to'excessive acid 'enzyme inhibition; and an optimal reaction microenvironment is provided for salivary enzyme activity, and multi-level taste experience is also provided. The gel characteristics of Chinese yam mucoprotein and oligosaccharide are fused to form a sour slow-release matrix, so that sour release presents a dynamic curve of front fast-middle stable-rear slow, the flavor release time is prolonged, and the continuous perception of the oral cavity is enhanced. The enzymatic reaction in the oral cavity is prolonged.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The invention relates to a bacillus licheniformis host cell for increasing production of secretory protein. In particular, the present invention relates to a Bacillus licheniformis host having a genetic modification that results in an increased production of heterologous, non-natural, secretory enzymes. Specifically, the present invention relates to a Bacillus licheniformis host cell which expresses a) a first polypeptide having peptidyl-prolyl cis-trans isomerase (EC 5.2. 1.8) activity comprising the amino acid sequence as shown in SEQ ID NO: 1, or at least 81% identical to SEQ ID NO: 1, and b) a second polypeptide having amylase activity comprising the amino acid sequence as shown in SEQ ID NO: 1, or at least 81% identical to SEQ ID NO: 2. Wherein the second polypeptide is heterologous to the Bacillus licheniformis host cell.

The present invention relates to variants of an alpha-amylase which have an increased exoamylase activity compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol or syrups and as viscosity breaker in oilfield and mining industries.

A Saccharomycopsis fibuligera strain, a microbial agent, a fermentation product, preparation and a use. The Saccharomycopsis fibuligera strain is preserved in the China Center for Type Culture Collection on 25 September 2023, with the preservation number of CCTCC NO: M20231788. The Saccharomycopsis fibuligera strain can be used to produce multiple enzymes required by fermentation, such as amylase, protease, and cellulase, wherein the amylase has the activities of pullulanase and raw starch amylase, can well adapt to special environmental conditions for fermentation, and has high environmental tolerance and high aroma production capability, thereby improving the quality of fermentation products, and reducing investment costs in fermentation production.

The application provides a composition for improving intestinal digestive enzyme activity and application thereof, and belongs to the technical field of animal medicine. The composition is composed of double-kidney polysaccharide, ginsenoside Rb1 and ginsenoside Rg1. In the composition, the mass ratio of the double-kidney polysaccharide, the ginsenoside Rb1 and the ginsenoside Rg1 is 90:5:5 or 95:2.5:2.5, preferably 90:5:5, and the intestinal digestive enzyme is specifically duodenal trypsin and amylase. Experimental results show that the composition can significantly synergistically improve the activities of the duodenal trypsin and amylase of laying hens, improve the feed utilization rate, thereby improve the egg production rate, improve the egg weight and the eggshell strength, and effectively improve the breeding benefit of the laying hens.

The chemical formula of a metal organic framework 2D-NCM (Fe) in the magnetic metal-organic framework mediated multi-enzyme nano material GOx-GAA at 2D-NCM (Fe) is Fe3O6Co2Ni2C3N0.4, Gox is glucose oxidase, and GAA is glucosidase. The invention also provides a method for efficiently and quickly measuring the activity of alpha-amylase, which comprises the following steps: acquiring a to-be-measured sample solution containing alpha-amylase, and carrying out enzymolysis on the to-be-measured sample solution and a starch solution at a first preset temperature for a first preset time to obtain a first reaction solution, after the enzymolysis reaction is finished, adding a first solution containing a magnetic metal-organic framework mediated multienzyme nanomaterial GOx-GAA at 2D-NCM (Fe) and a 3, 3 ', 5, 5'-tetramethyl benzidine solution into the first reaction solution, and reacting for a second preset time to obtain a second reaction solution; measuring the absorbance of the second reaction liquid at 652 nm; and obtaining the enzymatic activity of the alpha-amylase in the to-be-detected sample solution containing the alpha-amylase according to a standard curve of association between the absorbance at 652nm and the enzymatic activity of the alpha-amylase.

This solution discloses a high-temperature fermentation strain for Maotai-flavor baijiu brewing and its application in the field of functional microbial technology. The strain was isolated from mature Maotai-flavor baijiu mother koji and identified as *Bacillus cereus* XJ143 by 16S rRNA sequencing, possessing high cellulase activity. This strain is heat-resistant and suitable for the Maotai-flavor step-by-step koji-making process, solving industry problems such as insufficient decomposition of raw materials in traditional koji, insufficient pyrazine roasting aroma, and large batch-to-batch quality fluctuations in naturally produced koji. It can directly prepare liquid seed culture or solid inoculum for mother koji expansion, high-temperature koji, and mash fermentation, exhibiting strong adaptability for industrial production. *Cropensis ivory* XJ112 possesses high protease and amylase activity. The combination of *Bacillus cereus* XJ143 and *Cropensis ivory* XJ112 combines the secretion abilities of protease, amylase, and cellulose, efficiently breaking down wheat cell walls and simultaneously decomposing raw starch and protein.

The present invention relates to the field of microorganisms, and discloses a Lactobacillus acidophilus and its application in the preparation of drugs for preventing and / or treating hyperlipidemia and hyperglycemia. The preservation number of the Lactobacillus acidophilus is CGMCC No. 33155. This strain can produce lactic acid, be acid-tolerant and bile-salt tolerant, and has strong auto-aggregation ability and hydrophobic ability. The strain provided by the present invention can promote the decomposition of fat and sugar by degrading cholesterol and inhibiting the activities of α-glucosidase and α-amylase, and can reduce the blood glucose, blood lipid and inflammatory factor expression levels of hyperlipidemic and hyperglycemic mice, providing a new idea for the preparation of drugs for preventing and / or treating hyperglycemia and hyperlipidemia.

The invention belongs to the technical field of biology, and particularly relates to a bifidobacterium longum subsp. Longum FMBL B241768LS, a microbial inoculum and application of the microbial inoculum in hypoglycemic products, the bifidobacterium longum subsp. Longum FMBL B241768LS is preserved in China Center for Type Culture Collection on October 14, 2024, and the preservation number is CCTCC NO: M 20242173; the inhibitory activity of the compound on dipeptidyl peptidase IV is as high as 79.17%; the compound has a relatively good inhibition effect on the activity of alpha-glucosidase and alpha-amylase; the compound shows drug resistance to ciprofloxacin, ampicillin, penicillin G, clindamycin, kanamycin and polymyxin B; a good inhibition effect is achieved on pathogenic bacteria; the strain can be used for preparing medicines, fermented foods, health-care products and food additives for reducing blood sugar and inhibiting pathogenic bacteria, and has a wide application prospect.

Lateral flow devices (LFDs) and insoluble fluorescence (FAM)-labeled starch substrates, compositions comprising the same, and methods for use thereof in the measurement of alpha amylase activity in a sample.

The present invention relates to a Bacillus licheniformis host cell for increased production of secreted proteins. Specifically, the invention relates to a Bacillus licheniformis host with a genetic modification that leads to increased production of a heterologous, non-native, secreted enzyme. Specifically, the present invention relates to a Bacillus licheniformis host cell expressing a) a first polypeptide having peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) activity, said first polypeptide comprising an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence being a least 81% identical to SEQ ID NO: 1, and b) a second polypeptide having amylase activity, wherein said second polypeptide is heterologous to said Bacillus licheniformis host cell.

The present invention relates to isolated polypeptides having alpha-amylase activity, polypeptides comprising an alpha-amylase catalytic domain and a carbohydrate binding module, and polynucleotides encoding the polypeptides, compositions comprising said polypeptides, as well as polynucleotides and nucleic acid constructs or vectors encoding the polypeptides, and host cells comprising the polynucleotides or constructs / vectors, as well as methods of producing and using the polypeptides.

The invention discloses application of a medicago sativa MsBAM41 gene (SEQ.ID.NO.1) in improvement of saline-alkali resistance and low-temperature composite stress resistance of plants, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene is shown as SEQ. ID. NO.1 (the full length is 1722 bp). The amino acid sequence of the protein coded by the medicago sativa MsBAM41 gene is as shown in SEQ.ID.NO. 2. According to the invention, MsBAM41-RNAi alfalfa is created, and the MsBAM41 expression of the MsBAM41-RNAi alfalfa is obviously reduced. Under composite stress, compared with a wild type plant and a transgenic plant, the wilting damage is heavier, the beta-amylase activity and the soluble sugar content are remarkably reduced, the electrolyte permeability and the malondialdehyde content are remarkably improved, and the membrane damage is aggravated. It is proved that MsBAM41 is crucial to resistance to composite stress, a key gene is provided for creating stress-tolerant medicago sativa by means of a genetic engineering technology, and the application prospect is wide.

The invention relates to the technical field of plant growth regulation, in particular to a cultivation method for promoting germination of wild sea-buckthorn seeds, and the main invention concept is that amino-oligosaccharin (AS) and sodium nitroprusside (SNP) are synergistically applied to promoting germination of the wild sea-buckthorn seeds and extension of hypocotyl. According to the present invention, the germination rate (GP) and the germination index (GI) of the sea-buckthorn seeds can be significantly improved by separately using AS (the optimal concentration is 0.12 mmol / L) or SNP (the optimal concentration is 0.09 mmol / L) while the elongation of hypocotyl is promoted, and the AS (0.12 mmol / L) and the SNP (0.08 mmol / L) are mixed according to the ratio of 1: 1 and then are subjected to combined treatment so as to produce the synergistic effect, promote the germination of the wild sea-buckthorn seeds, improve the alpha-amylase activity of the seeds and promote the elongation of hypocotyl; the invention provides a low-cost and high-benefit technical scheme for efficient seedling raising of sea-buckthorn, and is suitable for ecological restoration and economic forest cultivation.

This invention relates to a Bacillus velezensis strain and mutants thereof with surprisingly advantageous properties for a beneficial cleaning effect. These Bacillus strains are particularly good enzyme producers with high protease activity, in particular protease activity and cellulase activity, preferably protease, cellulase, and amylase activity. The protease activity is in particular secreted protease activity. The invention further relates to preparations of these Bacillus strains, a cleaning formulation (F) comprising the Bacillus strains according to the invention, a method for cleaning an object (O) or the human or animal body (B) as well as the use of the cleaning formulation (F) in cleaning an object (O) or the human or animal body (B). It further relates to a feed or food (E) comprising the Bacillus strains according to the invention and a method of feeding an animal.

The application discloses application of calyculostero in preparation of blood sugar reducing drugs, and belongs to the technical field of pharmacological action of calyculostero. Experimental results show that the calyculostero can inhibit the activity of pancreatic alpha-amylase (IC50 is 4.34+ / -1.10 ug / mL) in a dose-dependent manner, reduce postprandial blood sugar, and improve sugar tolerance, reduce fasting blood sugar, improve total cholesterol and triglyceride levels, and reduce histopathological changes of liver, pancreas and kidney in long-term intervention, and has no obvious hepatotoxicity. The calyculostero can be used alone or together with a pharmaceutically acceptable excipient to prepare a pharmaceutical composition, and is used for reducing blood sugar or treating diabetes, and the dosage form includes oral preparations, injections and the like. The application provides a new natural medicine selection for drug treatment of diabetes.

The invention provides a method for improving disease-resistant activity through gradient enzymolysis coupling fermentation, and belongs to the technical field of biological fermentation. The method comprises the steps of raw material pretreatment, multi-stage gradient enzymolysis, enzymolysis-fermentation coupling and product post-treatment. The core innovation is that the activity of cellulase, protease and amylase is sequentially activated by precisely regulating and controlling the temperature to be 45-60 DEG C and the gradient change of pH to be 5.5-7.0, and oriented full degradation of raw material macromolecules is achieved; after enzymolysis, directly inoculating lactic acid bacteria-saccharomycetes composite flora for coupled fermentation, and supplementing a nitrogen source in batches. According to the method, the problems of insufficient enzymolysis, low active substance yield and the like of a traditional segmented process are solved, the active substance yield is increased to 2.1-2.4 times of that of the traditional process, the diameter of a pathogenic bacterium inhibition zone of the product reaches 24.8-26.3 mm, and the process is green and efficient, is suitable for industrial production and has a wide application prospect.

The invention relates to the technical field of agricultural microorganisms, in particular to bacillus rugosii and application thereof. The bacillus rugosus disclosed by the invention is preserved in the China Center for Type Culture Collection on October 25, 2023, and the preservation number of the bacillus rugosus is CCTCC (China Center for Type Culture Collection) NO: M20232022. The bacillus rugosii and the microbial agent provided by the invention have a very strong antagonistic effect on phytophthora capsici, and have a very good prevention and treatment effect on phytophthora blight of capsici; the ecological adaptability is high; the organic phosphorus decomposing effect is good, and the amylase decomposing activity and the cellulase decomposing activity are remarkable; the fertilizer has strong drought-resistant and saline-alkali-resistant capabilities, and can promote the growth of peppers under saline-alkali conditions, improve the agronomic traits of the peppers and promote the yield increase of the peppers.

The invention belongs to the technical field of biology, and particularly relates to a bifidobacterium longum subsp. Longum FMBL B241770LS, a microbial inoculum and application of the microbial inoculum in hypoglycemic products, the bifidobacterium longum subsp. Longum FMBL B241770LS is preserved in China Center for Type Culture Collection on September 14, 2024, and the preservation number is CCTCC NO: M 20241993; the inhibitory activity of the compound on dipeptidyl peptidase IV is up to 84.99%; the compound has a relatively good inhibition effect on the activity of alpha-glucosidase and alpha-amylase; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; the lactobacillus plantarum has good carbohydrate utilization capacity, can be used for preparing antioxidant products, hypoglycemic drugs, fermented food, health care products, food additives and feeds, and has wide application prospects.

This invention relates to the field of plant growth regulation technology, specifically disclosing a cultivation method for promoting the germination of wild sea buckthorn seeds. The main inventive concept lies in the synergistic application of amino oligosaccharides (AS) and sodium nitroprusside (SNP) in promoting the germination and hypocotyl elongation of wild sea buckthorn seeds. Using AS alone (optimal concentration 0.12 mmol / L) or SNP alone (optimal concentration 0.09 mmol / L) can significantly improve the germination rate (GP) and germination index (GI) of sea buckthorn seeds, while simultaneously promoting hypocotyl elongation. Combining AS (0.12 mmol / L) and SNP (0.08 mmol / L) in a 1:1 ratio produces a synergistic effect, promoting the germination of wild sea buckthorn seeds, increasing seed α-amylase activity, and promoting hypocotyl elongation. This invention provides a low-cost, high-efficiency technical solution for efficient sea buckthorn seedling cultivation, applicable to ecological restoration and economic forest cultivation.

The invention provides a preparation method and application of smallanthus sonchifolius polysaccharide YAPa-1, and belongs to the field of food biotechnology and natural polysaccharide development. A main chain in the molecular structural formula of the yacon polysaccharide YAPa-1 is formed by alternating galactose and mannose, and side chains are arabinose, glucose and rhamnose; the molar ratio of the galactose to the mannose to the glucose to the arabinose to the rhamnose is 1.00 to 0.82 to 0.25 to 0.11 to 0.25. The molecular weight of the smallanthus sonchifolius polysaccharide YAPa-1 is 10873 Da, the smallanthus sonchifolius polysaccharide YAPa-1 is of a triple helix structure, the microstructure of the smallanthus sonchifolius polysaccharide YAPa-1 is of a spongy porous network structure, and an alpha-glucosidase / alpha-amylase inhibition mechanism of the smallanthus sonchifolius polysaccharide YAPa-1 can According to the invention, the high-activity single yacon polysaccharide YAPa-1 is directionally obtained through cellulase-assisted hydrothermal extraction and a two-step column chromatography purification process. The smallanthus sonchifolius polysaccharide YAPa-1 can effectively inhibit the activity of alpha-glucosidase and alpha-amylase, the inhibition effect is close to that of a common hypoglycemic drug acarbose, and the smallanthus sonchifolius polysaccharide YAPa-1 is free of common side effects of synthetic drugs and can be applied to development of hypoglycemic products.